Preparation method and application of mirabilis jalapa extract

Abstract

The invention provides a preparation method and application of a mirabilis jalapa extract, and belongs to the technical field of skin care products. The method comprises the following steps: (1) crushing mirabilis jalapa until the particle size is 100-300 microns, and then carrying out steam explosion treatment to obtain steam-exploded mirabilis jalapa; (2) mixing the steam-exploded mirabilis jalapa with water, adding a compound enzyme preparation, and performing enzymolysis treatment to obtain enzymatic hydrolysate; and (3) carrying out concentration and freeze-drying treatment on the enzymatic hydrolysate to obtain the mirabilis jalapa extract. According to the method, the mirabilis jalapa is subjected to steam explosion treatment and then subjected to enzymolysis treatment through mannase, cellulase and xylanase, so that effective components in the mirabilis jalapa can be fully extracted into enzymatic hydrolysate, and the extraction efficiency is guaranteed.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and in particular relates to a preparation method of a Mirabilis jalapa extract and application thereof. Background Art

[0002] Mirabilis jalapa L. is a common plant widely distributed around the world. It not only has a certain ornamental value, but is also rich in a variety of bioactive ingredients, and has attracted attention in both traditional medicine and modern research. However, the current extraction methods for Mirabilis jalapa extracts may have low extraction rates or lead to the loss of active ingredients in the obtained extracts. For example, traditional water extraction methods and organic solvent extraction methods have problems such as low yields and organic solvent residues. Ultrasonic-assisted extraction methods and microwave-assisted extraction methods may lead to the inactivation of temperature-sensitive components. Therefore, there is an urgent need for a method for preparing a Mirabilis jalapa extract with high extraction rates and high active ingredients. Summary of the invention

[0003] In view of this, the object of the present invention is to provide a preparation method of Mirabilis jalapa extract and its application. The present invention steam-explodes Mirabilis jalapa and then enzymolyzes it with mannanase, cellulase and xylanase, so that the effective ingredients in Mirabilis jalapa can be fully extracted into the enzymolysis solution, thereby ensuring the extraction efficiency.

[0004] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0005] The present invention provides a method for preparing a Mirabilis jalapa extract, comprising the following steps:

[0006] (1) crushing Mirabilis jalapa to a particle size of 100 to 300 μm and then performing a steam explosion treatment to obtain steam-exploded Mirabilis jalapa;

[0007] (2) mixing the steam-exploded Mirabilis jalapa with water, adding a composite enzyme preparation, performing enzymolysis treatment, and obtaining an enzymolysis solution;

[0008] (3) The enzymatic hydrolysate is concentrated and freeze-dried to obtain the Mirabilis jalapa extract.

[0009] Preferably, the time of the steam explosion treatment in step (1) is 15 to 60 seconds, and the pressure of the steam explosion treatment is 0.5 to 3 MPa.

[0010] Preferably, the mass volume ratio of the steam-exploded Mirabilis jalapa to water in step (2) is 1 g: 1.5-5 mL.

[0011] Preferably, the composite enzyme preparation in step (2) is composed of xylanase, mannanase and cellulase in a mass ratio of 1:0.1 to 1:1 to 3.

[0012] Preferably, the added amount of the complex enzyme preparation is 0.05% to 0.2% of the mass of the steam-exploded Mirabilis jalapa.

[0013] Preferably, the enzymatic hydrolysis temperature is 35-45° C., and the enzymatic hydrolysis time is 0.5-1.5 h.

[0014] Preferably, the concentration temperature is 40-60° C., and the volume after concentration is 40%-60% of the original volume.

[0015] The present invention also provides the use of the Mirabilis jalapa extract obtained by the preparation method in the preparation of skin care products.

[0016] Preferably, the skin care products include facial masks, lotions, toners, facial creams and essences.

[0017] Preferably, the concentration of the Mirabilis jalapa extract in the skin care product is 1 to 10 mg / mL.

[0018] Compared with the prior art, the present invention has the following beneficial effects: the present invention performs steam explosion treatment on Mirabilis jalapa to change the structure of Mirabilis jalapa, so that the effective ingredients in Mirabilis jalapa are easier to extract while maintaining activity, and then performs enzymolysis treatment on Mirabilis jalapa by mannanase, cellulase and xylanase, so that the effective ingredients are fully extracted into the enzymolysis solution, thereby ensuring the extraction efficiency, and obtains Mirabilis jalapa extract by separating and freeze-drying the enzymolysis solution. The obtained Mirabilis jalapa extract is dried by freeze-drying technology, which can effectively maintain the activity of the effective ingredients in the extract. Whether the Mirabilis jalapa extract in liquid form or the freeze-dried Mirabilis jalapa extract is used, the effects of moisturizing, soothing, repairing, whitening and removing spots can be achieved. DETAILED DESCRIPTION

[0019] The present invention provides a method for preparing a Mirabilis jalapa extract, comprising the following steps:

[0020] (1) crushing Mirabilis jalapa to a particle size of 100 to 300 μm and then performing a steam explosion treatment to obtain steam-exploded Mirabilis jalapa;

[0021] (2) mixing the steam-exploded Mirabilis jalapa with water, adding a composite enzyme preparation, performing enzymolysis treatment, and obtaining an enzymolysis solution;

[0022] (3) The enzymatic hydrolysate is concentrated and freeze-dried to obtain the Mirabilis jalapa extract.

[0023] In the present invention, Mirabilis jalapa is crushed to a particle size of 100 - 300 μm and then subjected to steam explosion treatment to obtain steam-exploded Mirabilis jalapa. There are no special limitations on the crushing method in the present invention, and a conventional crushing method can be used. The particle size of the crushed Mirabilis jalapa is preferably 150 - 250 μm, more preferably 200 μm. The steam explosion treatment is carried out using a steam exploder. The time of steam explosion is 15 - 60 s, preferably 30 - 50 s, more preferably 40 s. The pressure of the steam explosion treatment is 0.5 - 3 MPa, preferably 1 - 2.5 MPa, more preferably 2 MPa.

[0024] In the present invention, the steam-exploded Mirabilis jalapa is mixed with water, a composite enzyme preparation is added, and enzymatic hydrolysis treatment is carried out to obtain an enzymatic hydrolysate. The mass-to-volume ratio of the steam-exploded Mirabilis jalapa to water is 1 g:1.5 - 5 mL, preferably 1 g:2.5 - 3.5 mL, more preferably 3 mL. The composite enzyme preparation is composed of xylanase, mannanase and cellulase in a mass ratio of 1:0.1 - 1:1 - 3, preferably 1:0.3 - 0.8:1.5 - 2.5, more preferably 1:0.5:2. The addition amount of the composite enzyme preparation is 0.05% - 0.2% of the mass of the steam-exploded Mirabilis jalapa, preferably 0.08% - 0.15%, more preferably 0.1%. The temperature of the enzymatic hydrolysis is 35 - 45 °C, preferably 38 - 42 °C, more preferably 40 °C. The time of the enzymatic hydrolysis is 0.5 - 1.5 h, preferably 0.75 h - 1.25 h, more preferably 1 h.

[0025] In the present invention, the enzymatic hydrolysate is concentrated and freeze-dried to obtain a Mirabilis jalapa extract. The temperature of the concentration is 40 - 60 °C, preferably 45 - 55 °C, more preferably 50 °C. The volume after concentration is 40% - 60% of the original volume, preferably 45% - 55%, more preferably 50%. For the freeze-drying treatment: the enzymatic hydrolysate is pre-frozen at -30 °C to -40 °C for 2 - 4 h to obtain the frozen enzymatic hydrolysate, and the frozen enzymatic hydrolysate is placed in a drying chamber, and the vacuum degree in the drying chamber is controlled to be 10 - 30 Pa, and vacuum drying is carried out for 20 - 28 h. The temperature of the pre-freezing is preferably -32 °C to -38 °C, more preferably -35 °C, the time of the pre-freezing is preferably 2.5 - 3.5 h, preferably 3 h, the vacuum degree is preferably 15 - 25 Pa, more preferably 30 Pa, and the time of the vacuum drying is preferably 22 - 26 h, more preferably 24 h.

[0026] The present invention also provides the application of the Mirabilis jalapa extract obtained by the above preparation method in the preparation of skin care products.

[0027] In the present invention, the skin care products include facial masks, lotions, toners, creams, and essences; the concentration of Mirabilis jalapa extract in the skin care products is 1 to 10 mg / mL. Adding the Mirabilis jalapa extract prepared by the method of the present invention to the skin care products can make the obtained products have the effects of moisturizing, soothing and repairing, and whitening and freckle removing.

[0028] The technical solutions provided by the present invention will be described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0029] Example 1

[0030] (1) Put the dried Mirabilis jalapa into a pulverizer and pulverize it to a particle size of 200 μm. Put the pulverized Mirabilis jalapa into the material bin of the steam explosion machine, tighten the bin cover, set the explosion pressure to 2 MPa, wait until the internal pressure reaches the set pressure, maintain for 40 s, then open the pneumatic valve to complete the steam explosion, and collect the product after steam explosion in the material bin, which is the steam-exploded Mirabilis jalapa;

[0031] (2) After drying the steam-exploded Mirabilis jalapa in an oven at 45 °C, weigh 100 g of Mirabilis jalapa and mix it with 300 mL of water, add 0.1 g of a composite enzyme preparation (xylanase: mannanase: cellulase = 1:0.5:2, all purchased from Guangzhou Youke Biotechnology Co., Ltd.), and enzymolyze at 40 °C for 1 h to obtain an enzymolyzed solution;

[0032] (3) Concentrate the enzymolyzed solution under reduced pressure at 50 °C. After concentrating to 50% of the original volume of the enzymolyzed solution, pre-freeze the concentrated enzymolyzed solution at -35 °C for 3 h, and then place it in a drying chamber with a vacuum degree of 30 Pa and dry for 24 h to obtain Mirabilis jalapa extract.

[0033] Example 2

[0034] (1) Put the dried Mirabilis jalapa into a pulverizer and pulverize it to a particle size of 100 μm. Put the pulverized Mirabilis jalapa into the material bin of the steam explosion machine, tighten the bin cover, set the explosion pressure to 3 MPa, wait until the internal pressure reaches the set pressure, maintain for 15 s, then open the pneumatic valve to complete the steam explosion, and collect the product after steam explosion in the material bin, which is the steam-exploded Mirabilis jalapa;

[0035] (2) After drying the steam-exploded Mirabilis jalapa in an oven at 45 °C, weigh 100 g of Mirabilis jalapa and mix it with 150 mL of water, add 0.05 g of a composite enzyme preparation (xylanase: mannanase: cellulase = 1:0.5:2, all purchased from Guangzhou Youke Biotechnology Co., Ltd.), and enzymolyze at 35 °C for 1.5 h to obtain an enzymolyzed solution;

[0036] (3) The enzymolysis solution was concentrated under reduced pressure at 50 °C. After being concentrated to 60% of the original volume of the enzymolysis solution, the concentrated enzymolysis solution was pre-frozen at -35 °C for 3 h and then placed in a drying chamber with a vacuum degree of 30 Pa for 24 h to obtain Mirabilis jalapa extract.

[0037] Example 3

[0038] (1) The dried Mirabilis jalapa was put into a pulverizer and pulverized to a particle size of 300 μm. The pulverized Mirabilis jalapa was put into the material bin of the steam explosion machine, the bin cover was tightened, the explosion pressure was set to 0.5 MPa, and after the internal pressure reached the set pressure, it was maintained for 60 s and then the pneumatic valve was opened to complete the steam explosion, and the product after steam explosion in the material bin was collected, which was steam-exploded Mirabilis jalapa;

[0039] (2) After the steam-exploded Mirabilis jalapa was dried in an oven at 45 °C, 100 g of Mirabilis jalapa was weighed and added to 500 mL of water and mixed. 0.2 g of a composite enzyme preparation (xylanase: mannanase: cellulase = 1:0.5:2, all purchased from Guangzhou Youke Biotechnology Co., Ltd.) was added and enzymolyzed at 45 °C for 0.5 h to obtain an enzymolysis solution;

[0040] (3) The enzymolysis solution was concentrated under reduced pressure at 50 °C. After being concentrated to 40% of the original volume of the enzymolysis solution, the concentrated enzymolysis solution was pre-frozen at -35 °C for 3 h and then placed in a drying chamber with a vacuum degree of 30 Pa for 24 h to obtain Mirabilis jalapa extract.

[0041] Comparative Example 1

[0042] Mirabilis jalapa extract was obtained in the same manner as in Example 1, except that the pulverized particle size of Mirabilis jalapa was 50 μm.

[0043] Comparative Example 2

[0044] Mirabilis jalapa extract was obtained in the same manner as in Example 1, except that the pulverized particle size of Mirabilis jalapa was 400 μm.

[0045] Comparative Example 3

[0046] Mirabilis jalapa extract was obtained in the same manner as in Example 1, except that the explosion pressure was set to 0.25 MPa.

[0047] Comparative Example 4

[0048] Mirabilis jalapa extract was obtained in the same manner as in Example 1, except that the explosion pressure was set to 4 MPa.

[0049] Comparative Example 5

[0050] Mirabilis jalapa extract was obtained in the same manner as in Example 1, except that the maintenance time was 5 s.

[0051] Comparative Example 6

[0052] The Mirabilis jalapa extract was obtained in the same manner as in Example 1, except that the retention time was 90 s.

[0053] Comparative Example 7

[0054] The Mirabilis jalapa extract was obtained in the same manner as in Example 1, except that the compound enzyme preparation used was composed of pectinase, mannanase and cellulase with a mass ratio of 1:0.5:2 (all purchased from Guangzhou Youke Biotechnology Co., Ltd.).

[0055] Comparative Example 8

[0056] (1) The dried Mirabilis jalapa was put into a pulverizer and pulverized to a particle size of 200 μm. 100 g of Mirabilis jalapa was weighed, added to 300 mL of water and mixed, and 0.1 g of a compound enzyme preparation (xylanase: mannanase: cellulase = 1:0.5:2, all purchased from Guangzhou Youke Biotechnology Co., Ltd.) was added. Under ultrasonic conditions, enzymatic hydrolysis was carried out at 40 °C for 1 h to obtain an enzymatic hydrolysate;

[0057] (2) The enzymatic hydrolysate was concentrated under reduced pressure at 50 °C. After being concentrated to 50% of the original volume of the enzymatic hydrolysate, the concentrated enzymatic hydrolysate was pre-frozen at -35 °C for 3 h and then placed in a drying chamber with a vacuum degree of 30 Pa and dried for 24 h to obtain the Mirabilis jalapa extract.

[0058] Comparative Example 9

[0059] (1) The dried Mirabilis jalapa was put into a pulverizer and pulverized to a particle size of 200 μm. The pulverized Mirabilis jalapa was put into the material bin of the steam explosion machine, the bin cover was tightened, the explosion pressure was set to 2 MPa, and after the internal pressure reached the set pressure, it was maintained for 40 s and then the pneumatic valve was opened to complete the steam explosion. The product after steam explosion in the material bin was collected, which was the steam-exploded Mirabilis jalapa;

[0060] (2) The steam-exploded Mirabilis jalapa was dried in an oven at 45 °C, then 100 g of Mirabilis jalapa was weighed, added to 300 mL of water and mixed, and ultrasonic treatment was carried out at 40 °C for 1 h to obtain an extract;

[0061] (3) The extract was concentrated under reduced pressure at 50 °C. After being concentrated to 50% of the original volume of the extract, the concentrated extract was pre-frozen at -35 °C for 3 h and then placed in a drying chamber with a vacuum degree of 30 Pa and dried for 24 h to obtain the Mirabilis jalapa extract.

[0062] Experimental Example 1

[0063] The yields of the Mirabilis jalapa extracts obtained in Examples 1-3 and Comparative Examples 1-9 of the present invention were calculated, and the results are shown in Table 1.

[0064] Yield (%) = Mirabilis jalapa extract / Weight of Mirabilis jalapa taken × 100%.

[0065] Table 1 Effects of different treatment groups on the yield of Mirabilis jalapa extract

[0066] Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Yield / % 19.17 18.84 19.06 14.52 15.11 13.67 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Yield / % 14.03 12.97 14.68 13.39 11.16 12.50

[0067] As can be seen from Table 1, the yields of Examples 1-3 are higher than those of Comparative Examples 1-9, indicating that the method of the present invention can effectively extract the active ingredients from Mirabilis jalapa, and changes in the particle size of the sample, blasting pressure, time, composition of the complex enzyme preparation, and extraction method also have an impact on the extraction effect.

[0068] Experimental Example 2

[0069] The maximum safe concentration of the Mirabilis jalapa extract prepared in Example 1 was determined by the MTT method.

[0070] Cell seeding: Adjust the density of mouse RAW264.7 macrophages (purchased from Wuhan Punosai Life Science Co., Ltd.) to 1×10 5 cell / mL, and inoculate 200 μL (1×10 4 cell / well) into a 96-well plate per well. Add basal medium around the 96-well plate to prevent edge effects. Another zero control group (200 μL of basal medium without cells) is set up and cultured in an incubator (37 °C, 5% CO2).

[0071] Sample preparation: Use DMEM basal medium to prepare a solution of Mirabilis jalapa extract with a concentration of 30 mg / mL, filter and sterilize it with a 0.22 μm filter membrane. Take it out before use and dilute it with DMEM medium into test sample solutions with concentrations of 0.5 mg / mL, 1 mg / mL, 5 mg / mL, 10 mg / mL, and 15 mg / mL for standby.

[0072] MTT: Weigh an appropriate amount of MTT powder precisely, dissolve it in PBS and prepare a concentration of 5 mg / mL, filter it with a 0.22 μm filter membrane and store it in the dark at 4 °C for standby. The validity period is 15 days.

[0073] Drug administration: Take out the 96-well plate after culturing for 24 h, discard the old medium, and plate it according to 3 replicates per concentration per group; add 200 μL of DMEM basal medium to each well of the zero control group and the blank control group, and add 200 μL of test sample solutions with different concentrations to each well of the experimental group, then put it back into the incubator for culture (37 °C, 5% CO2).

[0074] MTT assay for cell viability: 24 h after drug treatment, take out the 96-well plate, add 20 μL of MTT (5 mg / mL) to each well, put it back into the incubator and continue to culture for 4 h. Then discard the liquid in the wells, add 150 μL of DMSO to each well again, shake for 10 min, and measure the absorbance (OD value) at a wavelength of 560 nm.

[0075] Treatment of experimental results: Cell viability % = (OD value of experimental group - OD value of zero-adjustment well) / (OD value of blank control group - OD value of zero-adjustment group) × 100%. When the cell viability ≥ 90%, the corresponding sample concentration is the maximum safe concentration. The test results are shown in Table 2.

[0076] Table 2 Experimental results of the effect of Mirabilis jalapa extract on macrophage viability

[0077]

[0078]

[0079] As can be seen from Table 2, when the concentration of Mirabilis jalapa extract is 10 mg / mL, the cell viability is 90.34%, indicating that the maximum safe concentration of the cells is 10 mg / mL.

[0080] Experimental Example 3 Test for soothing and repairing efficacy

[0081] Test subjects: Female volunteers with sensitive facial skin, specifically manifested as one or more skin problems such as flushing, burning, dry peeling, itching and stinging on the facial skin. A total of 190 qualified volunteers (randomly divided into 19 groups, 10 people in each group), aged 18 - 45 years old. The test design is a randomized double-blind, historical control.

[0082] Product usage requirements: Volunteers use an aqueous solution with a concentration of 10 mg / mL prepared from the samples obtained in Examples 1 - 3 and Comparative Examples 1 - 9, or placebo control, on the whole face after cleansing in the morning and evening every day for 4 consecutive weeks. Questionnaire surveys on the usage effects and skin moisture content tests are conducted at specific time points.

[0083] (1) Consumer usage test

[0084] Test method: Volunteers conduct a questionnaire survey 4 weeks after using the product.

[0085] Effect judgment criteria:

[0086] Cured: The product is significantly effective and the skin allergy state is completely improved

[0087] Effective: The product is effective and the skin allergy state is somewhat alleviated

[0088] Invalid: The product is invalid. The skin allergy condition has not improved and has even deteriorated.

[0089] Product usage method: Evenly coat on the face. The results are shown in Table 3.

[0090] Table 3 Anti-allergy and repair effects of Mirabilis jalapa extracts obtained by different methods

[0091]

[0092]

[0093] As can be seen from Table 3, the Mirabilis jalapa extract obtained by the method of this application has good soothing and repair effects. And when the Mirabilis jalapa extracts obtained by different processes are at the same concentration, their effects are also different.

[0094] (2) Skin moisture content test

[0095] Use the skin moisture content test probe Corneometer CM 825 of CK Company in Germany to test 13 subjects aged 18 - 45 years old. The inner sides of the forearms of the subjects should not use any cosmetics 2 - 3 days before using the test sample. The test area is 3 cm × 3 cm. The test sample is applied once at a dosage of 1 mg, and the sample is evenly coated in the test area. The subjects use the test sample on the test area every morning and evening during the test. Before each instrument test, the subjects need to clean the inner sides of their forearms uniformly. The cleaning method is to wipe them clean with a dry paper towel, then sit quietly in a standard room for 20 min, not drink water or beverages, expose the forearms, place them in a test state, and keep relaxed. The subjects continuously use the sample for 4 weeks, and return visits are made respectively 8 h, two weeks (W2), and four weeks (W4) after use (no application is made before the return visits on the days of W2 and W4. After the W2 return visit test, it is normally applied until the day before the W4 return visit), image shooting and probe testing are carried out, and comparisons are made with before use. The test results are shown in Table 4.

[0096] Table 4 Skin moisture content test results

[0097]

[0098]

[0099] From the results in Table 4, it can be seen that the moisturizing rates and continuous moisturizing effects of Examples 1 - 3 are better. There is no report of adverse events in this test. Among them, compared with before use, the data after 4 weeks of using the product obtained by the present invention show that the skin moisture content has been significantly improved, indicating that the product of the present invention has good moisturizing effects.

[0100] Experimental Example 4

[0101] Accurately weigh 0.00362 g of tyrosinase powder (Sigma), and make up to 50 mL with PBS phosphate buffer solution at pH 6.8 to prepare sample test solutions with concentrations of 10, 100, 200, 500, 1000, and 1500 μg / mL obtained in Example 1. Add the sample test solutions according to Table 5, and then place them in a water bath at 37 °C for 10 min for constant temperature; then add tyrosinase to tubes A and C in sequence, with an interval of 30 s between each enzyme addition. After adding the enzyme, immediately vortex for 15 s to mix evenly, and react for 15 min. After the reaction tubes return to room temperature, measure the absorbance at 475 nm with an ultraviolet spectrophotometer to detect the inhibition rate of the sample on tyrosinase. The results are shown in Table 6, and the calculation formula for the inhibition rate of the sample on tyrosinase is as follows.

[0102] Inhibition rate (%) = [(A - B) - (C - D)] / (A - B) × 100%,

[0103] where A is the absorbance of the blank sample with the enzyme system; B is the absorbance of the blank sample without the enzyme system; C is the absorbance of the sample group with the enzyme system; D is the absorbance of the sample group without the enzyme system.

[0104] Table 5 Reagent addition table for tyrosine detection

[0105] Unit (μL) A B C D L - Tyrosine 800 800 800 800 Sample 0 0 800 800 PBS 1500 2000 700 1200 Tyrosinase 500 0 500 0 Total volume 2800 2800 2800 2800

[0106] Table 6 Inhibition rates of different concentrations on tyrosinase

[0107] Concentration / (μg / mL) 10 100 200 500 1000 1500 Inhibition rate / % 5.32 26.89 52.13 84.63 100 100

[0108] As can be seen from Table 6, the Mirabilis jalapa extract obtained by the present invention has the effect of inhibiting tyrosinase, indicating that the Mirabilis jalapa extract has the efficacy of whitening, and with the increase in the concentration of the Mirabilis jalapa extract, the whitening effect is more significant.

[0109] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A method for preparing Mirabilis jalapa extract, characterized in that, The following steps are involved: (1) crushing Mirabilis jalapa to a particle size of 100 to 300 μm and then performing a steam explosion treatment to obtain steam-exploded Mirabilis jalapa; (2) mixing the steam-exploded Mirabilis jalapa with water, adding a composite enzyme preparation, performing enzymolysis treatment, and obtaining an enzymolysis solution; (3) The enzymatic hydrolysate is concentrated and freeze-dried to obtain the Mirabilis jalapa extract.

2. The preparation method according to claim 1, wherein, The time of the steam explosion treatment in step (1) is 15 to 60 seconds, and the pressure of the steam explosion treatment is 0.5 to 3 MPa.

3. The preparation method according to claim 1, wherein The mass volume ratio of the steam-exploded Mirabilis jalapa to water in step (2) is 1 g: 1.5-5 mL.

4. The preparation method according to claim 1, characterized in that, The composite enzyme preparation in step (2) is composed of xylanase, mannanase and cellulase in a mass ratio of 1:0.1 to 1:1 to 3.

5. The preparation method according to claim 4, characterized in that, The added amount of the composite enzyme preparation is 0.05% to 0.2% of the mass of the steam-exploded Mirabilis jalapa.

6. The preparation method according to claim 1, wherein The temperature of the enzymolysis is 35-45° C., and the time of the enzymolysis is 0.5-1.5 h.

7. The preparation method according to claim 1, characterized in that, The concentration temperature is 40-60° C., and the volume after concentration is 40%-60% of the original volume.

8. Use of the Mirabilis jalapa extract obtained by the preparation method according to any one of claims 1 to 7 in the preparation of skin care products.

9. The application according to claim 8, wherein The skin care products include facial masks, emulsions, lotions, facial creams and essences.

10. The application according to claim 9, wherein, The concentration of the Mirabilis jalapa extract in the skin care product is 1-10 mg / mL.

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