Traditional Chinese medicine composition with digestion promoting function and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine composition with a digestion promoting function and a preparation method thereof, and the composition comprises the following components in parts by weight: 4-6 parts of pueraria flower, 4-6 parts of cistanche, 8-10 parts of sophora flower, 4-6 parts of wild buckwheat rhizome and 8-10 parts of fructus chebulae immaturus. The traditional Chinese medicine composition has the characteristics of simple process, convenience in operation, strong digestion promoting effect and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition with a digestive function promoting effect and a preparation method thereof. Background Art

[0002] Promoting digestive function refers to the process of improving the digestive efficiency of the gastrointestinal tract through drugs, foods, or specific therapies, and relieving symptoms such as indigestion, flatulence, and loss of appetite. Its core mechanisms include stimulating the secretion of digestive enzymes, enhancing gastrointestinal motility, and regulating the intestinal flora. In traditional Chinese medicine theory, digestive function is closely related to the "spleen and stomach", and it is believed that weakness of the spleen and stomach is the main cause of indigestion, and it is necessary to regulate with a combination of traditional Chinese medicines for strengthening the spleen and stomach and promoting digestion and resolving stagnation.

[0003] The Chinese digestive drug market has been growing continuously in recent years, and the market scale has reached a significant level. With the improvement of consumers' health awareness and the change of lifestyle, the demand for digestive drugs continues to grow. The Chinese digestive health product industry market also shows a rapid development trend, with a market scale exceeding 140 billion yuan and a year-on-year growth of 4.5%. Internationally, many important achievements have been made in the research of digestive drugs, including the research and development of new digestive drugs and the in-depth study of the drug action mechanism. The research of digestive enzymes is also one of the international hotspots. For example, the role of digestive enzymes in promoting digestion and improving nutrient absorption has been widely studied.

[0004] Flos Puerariae, also known as Gehua, is the dried flower of Pueraria lobata (Willd.) Ohwi and Pueraria thomsonii Benth. of the Leguminosae family. Pueraria lobata is distributed throughout the country (except Xinjiang and Tibet), and Pueraria thomsonii is distributed in Guangdong, Guangxi, Sichuan, Yunnan and other places1. Flos Puerariae is generally harvested when the flowers are not fully open after the Beginning of Autumn, the stalks, leaves and soil are removed, and then dried for later use. It is cool in nature, sweet (or slightly bitter) in taste, and belongs to the liver, lung, spleen and stomach meridians. It has the effects of relieving hangover and invigorating the spleen, clearing heat and protecting the liver, antioxidant, and promoting digestion.

[0005] Cistanche deserticola, also known as Roucongrong and Desert Ginseng, is a perennial parasitic herb of the genus Cistanche in the Orobanchaceae family, mainly distributed in desert areas such as Alashan in Inner Mongolia, Gansu, and Xinjiang. It parasitizes on the roots of Haloxylon ammodendron or Tamarix ramosissima, grows in a harsh environment, needs to tolerate extreme drought and large temperature differences between day and night, and can survive underground for decades, and is known as the "desert treasure". As a traditional precious Chinese medicinal material, Cistanche deserticola has a history of nearly 2,000 years of application. "Shennong Ben Cao Jing" and "Compendium of Materia Medica" both record its effects of "tonifying the kidney yang, benefiting essence and blood, and moistening the intestines for defecation", especially known for its characteristic of "tonifying without being drastic".

[0006] Sophora japonica Linn, also known as Robinia pseudoacacia, is a deciduous tree of the genus Sophora in the Leguminosae family. It is native to northern China, mainly distributed in the Loess Plateau and the North China Plain, and is now widely cultivated in various parts of the north and south. It is slightly cold in nature, bitter in taste, and enters the liver and large intestine meridians. It has the effects of cooling blood and stopping bleeding, clearing the liver and purging fire, anti-oxidation, and promoting digestion.

[0007] Fagopyrum dibotrys, also known as bitter buckwheat, wild bridge buckwheat, and sky buckwheat, is a perennial herbaceous plant of the Polygonaceae family. Its rhizome is lignified, with a brown surface and radial texture on the cross section. Its dried rhizome can be used as medicine, which is slightly spicy, astringent, and cool, and belongs to the lung meridian. It has the effects of clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, and discharging pus and removing blood stasis. It is mainly used to treat lung abscess, lung heat cough, scrofula, furuncle, sore throat and other symptoms. Fagopyrum dibotrys is rich in amylase activating substances, which can stimulate gastric acid secretion and accelerate food decomposition. Clinical studies have shown that its stomachic effect can improve symptoms such as loss of appetite and abdominal distension caused by spleen deficiency, and is especially suitable for people with indigestion to eat before meals.

[0008] Xiqingguo (Tibetan blueberry) is the dried young fruit of Terminalia chebula Retz., a plant of the Combretaceae family. Xiqingguo tastes bitter, sour and astringent, is neutral in nature, and enters the lung and large intestine meridians. It has the effects of clearing away heat, promoting body fluid, and detoxifying. It contains a large amount of dietary fiber, vitamin C and a variety of organic acids, which can promote intestinal peristalsis, improve digestive function, and help digestion and absorption of food, thereby relieving problems such as indigestion and abdominal distension. Summary of the invention

[0009] The purpose of the present invention is to provide a Chinese medicine composition with the function of promoting digestion and a preparation method thereof. The Chinese medicine composition has the characteristics of simple process, convenient operation, small side effects, strong digestion promoting effect, etc.

[0010] A Chinese medicinal composition with a digestion-promoting function comprises the following components by weight: 4-6 parts of kudzu flower, 4-6 parts of cistanche deserticola, 8-10 parts of sophora japonica flower, 4-6 parts of golden buckwheat and 8-10 parts of quinquefolia.

[0011] Preferably, the raw materials consist of the following components: 4-6 parts of kudzu flower, 4-6 parts of Cistanche deserticola, 8-10 parts of Sophora japonica flower, 4-6 parts of Fagopyrum scabra and 8-10 parts of Ophiopogon japonicus.

[0012] Preferably, the traditional Chinese medicine composition is prepared by extracting, concentrating, purifying, concentrating and drying raw materials.

[0013] The present invention also provides a method for preparing the above-mentioned Chinese medicine composition having the function of promoting digestion, comprising the following steps:

[0014] (1) Extraction: The raw materials are crushed and extracted with ethanol;

[0015] (2) Concentration and drying: Concentrate and dry the above-mentioned filtered extract under reduced pressure and vacuum.

[0016] (3) Preparation and purification: Take the extract obtained in step (2), dissolve it in ethanol, adsorb it on an adsorption column, elute it with 10 times of ultrapure water for 1 hour, elute it with 10 times of 35% ethanol for 1 hour, elute it with 10 times of 75% ethanol for 1 hour, and elute it with 95% ethanol for 1 hour, and collect the eluate.

[0017] (4) Concentration and drying: Concentrate and dry the above-mentioned collected 75% ethanol and ultrapure water eluate under reduced pressure and vacuum to obtain the product.

[0018] Preferably, in step (1), add 10 times of 75% ethanol solution, perform ultrasonic extraction 2 - 3 times at 450 - 500W for 80 - 90 minutes each time. After the extraction is completed, cool it to room temperature, filter, and combine the filtrates for standby.

[0019] Preferably, in step (2), the vacuum degree of reduced pressure and vacuum concentration is 150 mbar, and the temperature is 45 - 50 °C.

[0020] Preferably, in step (3), add the extract to 10 times of 75% ethanol for dissolution, and adsorb it on an AB - 8 resin column for 2 hours.

[0021] Preferably, in step (4), the vacuum degree of reduced pressure and vacuum concentration is 150 mbar, and the temperature is 45 - 50 °C to obtain a solid powder.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. Simple process and small side effects: Compared with other digestion - promoting products, the combinations used in the present invention are all combinations of natural products, without the need for synthesis and structural modification, reducing the generation of side effects on the human body. At the same time, there is no need to add additional auxiliary drugs, maintaining the pure natural source of the ingredients.

[0024] 2. High promotion rate: Compared with other digestion - promoting products, through high - throughput screening experiments on the digestion - promoting effect of pepsin by the traditional Chinese medicine composition of the present invention, it is found that it has a good pepsin activation effect and a significant digestion - promoting effect. Specific embodiments

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which the embodiments of the present invention belong. If the definitions stated in this section are contrary to or otherwise inconsistent with the definitions stated in the patents, patent applications, published patent applications, and other publications incorporated herein by reference, the definitions listed in this section shall prevail over the definitions incorporated herein by reference.

[0027] Unless otherwise specified, the methods used in the following examples are all conventional methods. The materials, reagents, and instruments used, unless otherwise specified, are all conventional materials, reagents, and instruments in this field, and those skilled in the art can obtain them through commercial channels. When an equivalent, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper limit preferred values and lower limit preferred values, it should be understood that all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value are specifically disclosed, regardless of whether the ranges are separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values and all integers and fractions within the range. In the specification and claims of this application, range limitations can be combined and / or interchanged, and these ranges include all sub-ranges contained therein unless otherwise stated.

[0028] The traditional Chinese medicine composition prepared in the following examples was subjected to high-throughput screening based on pepsin-promoted digestion. The high-throughput model based on pepsin-promoted digestion is as follows: Casein is decomposed by pepsin to generate tyrosine. When the Folin reagent undergoes an acid-base reaction with the benzene ring structure in the tyrosine molecule, purple or blue pigments will be generated, thus resulting in a color reaction. The depth of its color (absorbance) is proportional to the tyrosine content. Therefore, the degree of the effect of the sample on pepsin can be judged according to the tyrosine content.

[0029] Solution preparation:

[0030] (1) Trichloroacetic acid solution (0.4 mol / L): Accurately weigh 65.4 g of trichloroacetic acid, and make up to 1000 mL with deionized water.

[0031] (2) Sodium carbonate solution (0.4 mol / L): Accurately weigh 42.4 g of anhydrous sodium carbonate, dissolve it in deionized water, and make up to 1000 mL.

[0032] (3) Sodium hydroxide solution (0.1 mol / L): Accurately weigh 0.4 g of sodium hydroxide, and make up to 100 mL with deionized water.

[0033] (4) Hydrochloric acid solution (0.1 mol / L): Take 8.33 mL of concentrated hydrochloric acid (12 mol / L) in a volumetric flask, and add deionized water to make up the volume to 100 mL.

[0034] (5) Pepsin solution (stored at 4 °C): Accurately weigh 100 mg of pepsin, add 0.1 mol / L HCl solution to make up the volume to 10 mL.

[0035] (6) 2% Casein substrate buffer solution (stored at 4 °C): Accurately weigh 2 g of casein, add 40 mL of 0.1 mol / L sodium hydroxide, heat and dissolve it in a water bath (40 °C), and then make up the volume to 100 mL with deionized water.

[0036] (7) Tyrosine standard solution (stored at 4 °C): Accurately weigh 100 mg of tyrosine, add 0.1 M HCl solution until completely dissolved, and then add deionized water to make up the volume to 100 mL to obtain a 1 mg / mL tyrosine stock solution. When in use, dilute it with deionized water to 6 gradients of 10, 20, 40, 80, 160, 200 μg / mL for use.

[0037] (8) Folin-Ciocalteu reagent is purchased from Macklin P824172, and the concentration is 1 N.

[0038] Experimental operation and calculation:

[0039] Set up a blank group BLK, a sample group SPL, a sample control group SPLC, and a positive control group STD.

[0040] BLK / SPL: Take 80 μL of blank (water) and sample respectively in a 1.5 mL centrifuge tube, add 80 μL of casein solution and 80 μL of pepsin solution, mix well, incubate at 40 °C for 20 min, then add 160 μL of trichloroacetic acid solution, mix well and centrifuge for 5 min. Take 80 μL of the supernatant and add it to a 96-well plate, then add 20 μL of Folin-Ciocalteu reagent and 100 μL of sodium carbonate solution. After incubating at 40 °C for 20 min, read the absorbance value A at 680 nm BLK / A SPL . Set 3 parallel samples for each group.

[0041] SPLC: Take 80 μL of the centrifuged sample in a 96-well plate, then add 20 μL of ultrapure water and 100 μL of sodium carbonate solution. After incubating at 40 °C for 20 min, read the absorbance value A at 680 nm SPLC . Set 3 parallel samples for each group.

[0042] STD: Take 80 μL of tyrosine standard solutions at 10, 20, 40, 80, 160, and 200 μM each into a 96-well plate, then add 20 μL of Folin-Ciocalteu reagent solution and 100 μL of sodium carbonate solution. After incubating at 40 °C for 20 min, read the absorbance value A at 680 nm. STD . Set 3 parallel samples for each group.

[0043] Place the 96-well plate in a microplate reader for detection, and the procedure is as follows:

[0044]

[0045] Pepsin activity (u) = A SPL *K*N*0.2 / 20

[0046] A SPL A is the absorbance of the sample; K is the absorbance constant (calculate the amount of tyrosine (μg) when the absorbance is 1 according to the standard curve, which is the value of the absorbance constant K); N is the dilution factor (10 in this experiment); 0.2 is the reaction volume of 0.2 mL; 20 is the reaction time of 20 min.

[0047] Example 1

[0048] This example provides a preparation method of a traditional Chinese medicine composition for promoting digestive function, including the following steps:

[0049] (1) Extraction: The following components in parts by weight are used: 4 parts of Puerariae Flos, 4 parts of Cistanches Herba, 8 parts of Sophorae Flos, 4 parts of Fagopyri Dibotryis Rhizoma, and 8 parts of Terminaliae Myriophyllae Fructus are pulverized, added with 10 times of 75% ethanol solution, and ultrasonically extracted 2 times at 450 W for 80 min each time. After extraction, cool to room temperature, filter, and combine the filtrates. Concentration and drying: Concentrate and dry the above-collected fractions under reduced pressure in a vacuum at 45 °C;

[0050] (2) Preparation and purification: Take the above extract and dissolve it in 10 times of 75% ethanol, adsorb it on an AB-8 resin column for 2 hours, elute it with 10 times of ultrapure water for 1 hour, elute it with 10 times of 35% ethanol for 1 hour, elute it with 10 times of 75% ethanol for 1 hour, and elute it with 95% ethanol for 2 hours, and collect the eluate.

[0051] (3) Sample preparation: After concentrating and drying the 75% ethanol and ultrapure water eluates collected above under reduced pressure in a vacuum, dissolve them in 10 times of 75% ethanol.

[0052] (4) Detection of promoting digestive function: See the above for specific operations and calculations. The pepsin activity of this combination is 27.71 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 137.44%.

[0053] Example 2

[0054] This embodiment provides a preparation method of a traditional Chinese medicine composition for promoting digestive function, comprising the following steps:

[0055] (1) Extraction: The following components in parts by weight are used: 6 parts of Pueraria flower, 6 parts of Cistanche deserticola, 8 parts of Sophora japonica flower, 4 parts of Fagopyrum dibotrys, and 8 parts of Terminalia myriocarpa are pulverized, 10 times the amount of 75% ethanol solution is added, ultrasonic extraction is carried out 2 times at 475 W for 90 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum at a temperature of 45°C;

[0056] (2) Preparation and purification: The above extract is taken and dissolved in 10 times the amount of 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times the amount of ultrapure water for 1 hour, eluted with 10 times the amount of 35% ethanol for 1 hour, eluted with 10 times the amount of 75% ethanol for 1 hour, and eluted with 95% ethanol for 2 hours, and the eluate is collected.

[0057] (3) Sample preparation: The collected 75% ethanol and ultrapure water eluates are concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times the amount of 75% ethanol.

[0058] (4) Detection of promoting digestive function: For the specific operation and calculation, see above. The pepsin activity of this combination is 28.56 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 144.73%.

[0059] Example 3

[0060] This embodiment provides a preparation method of a traditional Chinese medicine composition for promoting digestive function, comprising the following steps:

[0061] (1) Extraction: The following components in parts by weight are used: 4 parts of Pueraria flower, 4 parts of Cistanche deserticola, 10 parts of Sophora japonica flower, 6 parts of Fagopyrum dibotrys, and 10 parts of Terminalia myriocarpa are pulverized, 10 times the amount of 75% ethanol solution is added, ultrasonic extraction is carried out 2 times at 500 W for 80 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum at a temperature of 45°C;

[0062] (2) Preparation and purification: The above extract is taken and dissolved in 10 times the amount of 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times the amount of ultrapure water for 1 hour, eluted with 10 times the amount of 35% ethanol for 1 hour, eluted with 10 times the amount of 75% ethanol for 1 hour, and eluted with 95% ethanol for 2 hours, and the eluate is collected.

[0063] (3) Sample preparation: The collected 75% ethanol and ultrapure water eluates are concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times the amount of 75% ethanol.

[0064] (4) Detection of digestive function promotion: See the above for specific operations and calculations. The pepsin activity of this combination is 28.59 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 144.98%.

[0065] Example 4

[0066] This example provides a preparation method of a traditional Chinese medicine composition for promoting digestive function, including the following steps:

[0067] (1) Extraction: The following components in parts by weight are used: 6 parts of Puerariae Flos, 6 parts of Cistanches Herba, 10 parts of Sophorae Flos, 6 parts of Fagopyri Dibotryis Herba, and 10 parts of Terminaliae Chebulae Fructus. They are pulverized, and 10 times the amount of 75% ethanol solution is added. Ultrasonic extraction is carried out 3 times at 475W for 85 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum at a temperature of 45°C;

[0068] (2) Preparation and purification: The above extract is taken and dissolved in 10 times the amount of 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times the amount of ultrapure water for 1 hour, eluted with 10 times the amount of 35% ethanol for 1 hour, eluted with 10 times the amount of 75% ethanol for 1 hour, and eluted with 95% ethanol for 2 hours. The eluate is collected.

[0069] (3) Sample preparation: The 75% ethanol and ultrapure water eluates collected above are concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times the amount of 75% ethanol.

[0070] (4) Detection of digestive function promotion: See the above for specific operations and calculations. The pepsin activity of this combination is 27.58 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 136.33%.

[0071] Example 5

[0072] This example provides a preparation method of a traditional Chinese medicine composition for promoting digestive function, including the following steps:

[0073] (1) Extraction: The following components in parts by weight are used: 5 parts of Puerariae Flos, 5 parts of Cistanches Herba, 9 parts of Sophorae Flos, 5 parts of Fagopyri Dibotryis Herba, and 9 parts of Terminaliae Chebulae Fructus. They are pulverized, and 10 times the amount of 75% ethanol solution is added. Ultrasonic extraction is carried out 3 times at 500W for 90 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum at a temperature of 45°C;

[0074] (2) Preparation and purification: The extract was dissolved in 10 times 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times ultrapure water for 1 hour, eluted with 10 times 35% ethanol for 1 hour, eluted with 10 times 75% ethanol for 1 hour, eluted with 95% ethanol for 2 hours, and the eluate was collected.

[0075] (3) Sample preparation: The collected 75% ethanol and ultrapure water eluents were concentrated and dried under reduced pressure in a vacuum, and then 10 times 75% ethanol was added to dissolve.

[0076] (4) Digestive function test: The specific operation and calculation are as above. The pepsin activity of the combination is 28.1 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 140.78%.

[0077] Comparative Example 1

[0078] This comparative example provides a method for preparing a Chinese medicine composition for promoting digestive function, comprising the following steps:

[0079] (1) Extraction: The following components in parts by weight: 4 parts of Pueraria lobata flower, 4 parts of Cistanche deserticola, 8 parts of Sophora japonica flower, and 4 parts of Fagopyrum truncatum were crushed, 10 times of 75% ethanol solution was added, and ultrasonic extraction was performed twice at 450W, each time for 80 minutes. After the extraction, the extraction was cooled to room temperature, filtered, and the filtrate was combined. Concentration and drying: The above collected fractions were concentrated and dried under reduced pressure and vacuum at a temperature of 45°C;

[0080] (2) Preparation and purification: The extract was dissolved in 10 times 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times ultrapure water for 1 hour, eluted with 10 times 35% ethanol for 1 hour, eluted with 10 times 75% ethanol for 1 hour, eluted with 95% ethanol for 2 hours, and the eluate was collected.

[0081] (3) Sample preparation: The collected 75% ethanol and ultrapure water eluents were concentrated and dried under reduced pressure in a vacuum, and then 10 times 75% ethanol was added to dissolve.

[0082] (4) Digestive function test: The specific operation and calculation are as above. The pepsin activity of the combination is 23.7 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 103.08%.

[0083] Compared with Example 1, the pepsin activity promotion rate in Comparative Example 1 decreased from 137.44% to 103.08% due to the lack of Xiqingguo medicinal materials.

[0084] Comparative Example 2

[0085] This comparative example provides a method for preparing a Chinese medicine composition for promoting digestive function, comprising the following steps:

[0086] (1) Extraction: The following components in parts by weight are used: 4 parts of Pueraria flower, 4 parts of Cistanche deserticola, 8 parts of Sophora japonica flower, and 8 parts of Terminalia chebula Retz. var. tomentella Kurt. are pulverized, 10 times the amount of 75% ethanol solution is added, ultrasonic extraction is carried out at 450 W for 2 times, 80 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum, at a temperature of 45 °C;

[0087] (2) Preparation and purification: The above extract is taken and dissolved in 10 times the amount of 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times the amount of ultrapure water for 1 hour, eluted with 10 times the amount of 35% ethanol for 1 hour, eluted with 10 times the amount of 75% ethanol for 1 hour, eluted with 95% ethanol for 2 hours, and the eluate is collected.

[0088] (3) Sample preparation: The collected 75% ethanol and ultrapure water eluates are concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times the amount of 75% ethanol.

[0089] (4) Detection of digestion-promoting function: The specific operation and calculation are as above. The pepsin activity of this combination is 24.98 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 114.05%.

[0090] Compared with Example 1, in Comparative Example 2, after lacking the Fagopyrum dibotrys (D. Don) Hara medicinal material, the pepsin activity promotion rate decreased from 137.44% to 114.05%.

[0091] Comparative Example 3

[0092] This comparative example provides a preparation method of a traditional Chinese medicine composition for promoting digestion function, including the following steps:

[0093] (1) Extraction: The following components in parts by weight are used: 4 parts of Pueraria flower, 4 parts of Cistanche deserticola, 4 parts of Fagopyrum dibotrys (D. Don) Hara, and 8 parts of Terminalia chebula Retz. var. tomentella Kurt. are pulverized, 10 times the amount of 75% ethanol solution is added, ultrasonic extraction is carried out at 450 W for 2 times, 80 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum, at a temperature of 45 °C;

[0094] (2) Preparation and purification: The above extract is taken and dissolved in 10 times the amount of 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times the amount of ultrapure water for 1 hour, eluted with 10 times the amount of 35% ethanol for 1 hour, eluted with 10 times the amount of 75% ethanol for 1 hour, eluted with 95% ethanol for 2 hours, and the eluate is collected.

[0095] (3) Sample preparation: The collected 75% ethanol and ultrapure water eluates are concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times the amount of 75% ethanol.

[0096] (4) Detection of digestion-promoting function: The specific operation and calculation are as described above. The pepsin activity of this combination is 24.81 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 112.59%.

[0097] Compared with Example 1, after lacking Sophora japonica in Comparative Example 3, the pepsin activity promotion rate decreased from 137.44% to 112.59%.

[0098] Comparative Example 4

[0099] This comparative example provides a preparation method of a traditional Chinese medicine composition for promoting digestion function, including the following steps:

[0100] (1) Extraction: It is composed of the following components in parts by weight: 4 parts of Pueraria flos, 8 parts of Sophora japonica, 4 parts of Fagopyrum dibotrys, and 8 parts of Terminalia chebula Retz. var. khasiana (Hook. f.) C. E. C. Fisch. are pulverized, 10 times of 75% ethanol solution is added, ultrasonic extraction is carried out 2 times at 450W for 80 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The above-collected fractions are concentrated and dried under reduced pressure in a vacuum at a temperature of 45 °C;

[0101] (2) Preparation and purification: Take the above extract, dissolve it in 10 times of 75% ethanol, adsorb it on an AB-8 resin column for 2 hours, elute it with 10 times of ultrapure water for 1 hour, elute it with 10 times of 35% ethanol for 1 hour, elute it with 10 times of 75% ethanol for 1 hour, and elute it with 95% ethanol for 2 hours, and collect the eluate.

[0102] (3) Sample preparation: After the 75% ethanol and ultrapure water eluates collected above are concentrated and dried under reduced pressure in a vacuum, they are dissolved in 10 times of 75% ethanol.

[0103] (4) Detection of digestion-promoting function: The specific operation and calculation are as described above. The pepsin activity of this combination is 24.45 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 109.51%.

[0104] Compared with Example 1, after lacking Cistanche deserticola in Comparative Example 4, the pepsin activity promotion rate decreased from 137.44% to 109.51%.

[0105] Comparative Example 5

[0106] This comparative example provides a preparation method of a traditional Chinese medicine composition for promoting digestion function, including the following steps:

[0107] (1) Extraction: The following components by weight: 4 parts of Cistanche deserticola, 8 parts of Sophora japonica, 4 parts of Fagopyrum truncatum, and 8 parts of Ophiopogon japonicus were crushed, 10 times of 75% ethanol solution was added, and ultrasonic extraction was performed twice at 450W, each time for 80 minutes. After the extraction, the extraction was cooled to room temperature, filtered, and the filtrate was combined. Concentration and drying: The collected fractions were concentrated and dried under reduced pressure and vacuum at a temperature of 45°C;

[0108] (2) Preparation and purification: The extract was dissolved in 10 times 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times ultrapure water for 1 hour, eluted with 10 times 35% ethanol for 1 hour, eluted with 10 times 75% ethanol for 1 hour, eluted with 95% ethanol for 2 hours, and the eluate was collected.

[0109] (3) Sample preparation: The collected 75% ethanol and ultrapure water eluents were concentrated and dried under reduced pressure in a vacuum, and then 10 times 75% ethanol was added to dissolve.

[0110] (4) Digestive function test: The specific operation and calculation are as above. The pepsin activity of the combination is 24.98 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 114.05%.

[0111] Compared with Example 1, the pepsin activity promotion rate in Comparative Example 5 decreased from 137.44% to 114.05% due to the lack of Pueraria lobata flower medicinal material.

[0112] Comparative Example 6

[0113] This comparative example provides a method for preparing a Chinese medicine composition for promoting digestive function, comprising the following steps:

[0114] (1) Extraction: Weigh 10 portions of each of the five medicinal materials and grind them, add 10 times 75% ethanol solution, and extract them twice at 450W ultrasonic, each time for 80 minutes. After the extraction, cool to room temperature, filter, and combine the filtrate. Concentration and drying: Concentrate and dry the above collected fractions under reduced pressure and vacuum at 45°C;

[0115] (2) Preparation and purification: The extract was dissolved in 10 times 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times ultrapure water for 1 hour, eluted with 10 times 35% ethanol for 1 hour, eluted with 10 times 75% ethanol for 1 hour, eluted with 95% ethanol for 2 hours, and the eluate was collected.

[0116] (3) Sample preparation: After the collected 75% ethanol and ultrapure water eluents are concentrated and dried under reduced pressure, 10 times 75% ethanol is added to dissolve. (Ensure that the concentration of each sample is 0.1 g / mL)

[0117] (4) Digestive function test:

[0118] Pueraria flower: 23.23 (u), promotion rate: 99.05%

[0119] Cistanche deserticola: 24.83 (u), promotion rate: 112.76%

[0120] Sophora japonica flower: 24.33 (u), promotion rate: 108.48%

[0121] Fagopyrum dibotrys: 23.21 (u), promotion rate: 98.88%

[0122] Terminalia chebula Retz. var. tomentella Kurt.: 24.87 (u), promotion rate: 113.11%

[0123] Comparative Example 7

[0124] This comparative example provides a preparation method of a traditional Chinese medicine composition for promoting digestive function, including the following steps:

[0125] (1) Extraction: The following components in parts by weight are used: 4 parts of Pueraria flower, 4 parts of Cistanche deserticola, 8 parts of Sophora japonica flower, 4 parts of Fagopyrum dibotrys, and 8 parts of Terminalia chebula Retz. var. tomentella Kurt. are pulverized, 10 times the 75% ethanol solution is added, ultrasonic extraction is carried out at 450W for 2 times, 80 minutes each time, after extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum, at a temperature of 45°C;

[0126] (2) Preparation and purification: Take the above extract and dissolve it in 10 times the 75% ethanol, adsorb it on an AB-8 resin column for 2 hours, elute it with 10 times ultrapure water for 1 hour, elute it with 10 times 35% ethanol for 1 hour, elute it with 10 times 75% ethanol for 1 hour, elute it with 95% ethanol for 2 hours, and collect the eluate.

[0127] (3) Sample preparation: The 35% ethanol and 75% ethanol eluates collected above are concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times the 75% ethanol.

[0128] (4) Detection of promoting digestive function: The specific operation and calculation are as above. The pepsin activity of this combination is 26.9 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 130.5%.

[0129] Compared with Example 1, in Comparative Example 7, the sample preparation step is changed to combine the 35% and 75% components, and the pepsin activity promotion rate drops from 137.44% to 130.5%.

[0130] Comparative Example 8

[0131] This comparative example provides a preparation method of a traditional Chinese medicine composition for promoting digestive function, including the following steps:

[0132] (1) Extraction: It is composed of the following components in parts by weight: 4 parts of Puerariae Flos, 4 parts of Cistanches Herba, 8 parts of Sophorae Flos, 4 parts of Fagopyri Dibotryis Rhizoma, and 8 parts of Terminaliae Chebulae Fructus. They are pulverized, 10 times the 75% ethanol solution is added, ultrasonic extraction is carried out twice at 450W for 80 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum at a temperature of 45°C;

[0133] (2) Preparation and purification: Take the above extract, dissolve it in 10 times the 75% ethanol, adsorb it on an AB-8 resin column for 2 hours, elute it with 10 times ultrapure water for 1 hour, elute it with 10 times 35% ethanol for 1 hour, elute it with 10 times 75% ethanol for 1 hour, elute it with 95% ethanol for 2 hours, and collect the eluate.

[0134] (3) Sample preparation: The collected 35% ethanol and ultrapure water eluates are concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times the 75% ethanol.

[0135] (4) Detection of digestive function promotion: For the specific operation and calculation, see above. The pepsin activity of this combination is 25.51 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 118.59%.

[0136] Compared with Example 1, in Comparative Example 8, the sample preparation step was changed to combine the 35% and ultrapure water components, and the pepsin activity promotion rate decreased from 137.44% to 118.59%.

[0137] Comparative Example 9

[0138] This comparative example provides a preparation method of a traditional Chinese medicine composition for promoting digestive function, including the following steps:

[0139] (1) Extraction: It is composed of the following components in parts by weight: 4 parts of Puerariae Flos, 4 parts of Cistanches Herba, 8 parts of Sophorae Flos, 4 parts of Fagopyri Dibotryis Rhizoma, and 8 parts of Terminaliae Chebulae Fructus. They are pulverized, 10 times the 75% ethanol solution is added, ultrasonic extraction is carried out twice at 450W for 80 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum at a temperature of 45°C;

[0140] (2) Preparation and purification: Take the above extract, dissolve it in 10 times the 75% ethanol, adsorb it on an AB-8 resin column for 2 hours, elute it with 10 times ultrapure water for 1 hour, elute it with 10 times 35% ethanol for 1 hour, elute it with 10 times 75% ethanol for 1 hour, elute it with 95% ethanol for 2 hours, and collect the eluate.

[0141] (3) Sample preparation: The collected ultrapure water eluate is concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times the 75% ethanol.

[0142] (4) Detection of digestion-promoting function: For the specific operation and calculation, refer to the above. The pepsin activity of this combination is 21.13 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 81.06%.

[0143] Compared with Example 1, after the sample preparation step of Comparative Example 9 was changed to a single water component, the pepsin activity promotion rate decreased from 137.44% to 81.06%.

[0144] Comparative Example 10

[0145] This comparative example provides a preparation method of a traditional Chinese medicine composition for promoting digestion function, including the following steps:

[0146] (1) Extraction: It is composed of the following components in parts by weight: 4 parts of Puerariae Flos, 4 parts of Cistanches Herba, 8 parts of Sophorae Flos, 4 parts of Fagopyri Dibotryis Rhizoma, and 8 parts of Terminaliae Chebulae Fructus. They are crushed, 10 times of 75% ethanol solution is added, and ultrasonic extraction is carried out 2 times at 450W for 80 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum at a temperature of 45°C;

[0147] (2) Preparation and purification: Take the above extract and dissolve it in 10 times of 75% ethanol, adsorb it on an AB-8 resin column for 2 hours, elute it with 10 times of ultrapure water for 1 hour, elute it with 10 times of 35% ethanol for 1 hour, elute it with 10 times of 75% ethanol for 1 hour, and elute it with 95% ethanol for 2 hours, and collect the eluate.

[0148] (3) Sample preparation: The 75% ethanol eluate collected above is concentrated and dried under reduced pressure in a vacuum and then dissolved in 10 times of 75% ethanol.

[0149] (4) Detection of digestion-promoting function: For the specific operation and calculation, refer to the above. The pepsin activity of this combination is 26.8 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 129.64%.

[0150] Compared with Example 1, after the sample preparation step of Comparative Example 10 was changed to a single 75% component, the pepsin activity promotion rate decreased from 137.44% to 129.64%.

[0151] Comparative Example 11

[0152] This comparative example provides a preparation method of a traditional Chinese medicine composition for promoting digestion function, including the following steps:

[0153] (1) Extraction: It is composed of the following components in parts by weight: 5 parts of Puerariae Flos, 6 parts of Cistanches Herba, 2 parts of Sophorae Flos, 5 parts of Fagopyri Dibotryis Rhizoma, and 2 parts of Phyllanthi Emblicae Fructus are pulverized, 10 times of 75% ethanol solution is added, ultrasonic extraction is carried out at 450W for 2 times, 80 minutes each time. After extraction, it is cooled to room temperature, filtered, and the filtrates are combined. Concentration and drying: The collected fractions are concentrated and dried under reduced pressure in a vacuum, at a temperature of 45 °C;

[0154] (2) Preparation and purification: Take the above extract, dissolve it in 10 times of 75% ethanol, adsorb it on an AB-8 resin column for 2 hours, elute it with 10 times of ultrapure water for 1 hour, elute it with 10 times of 35% ethanol for 1 hour, elute it with 10 times of 75% ethanol for 1 hour, and elute it with 95% ethanol for 2 hours, and collect the eluate.

[0155] (3) Sample preparation: The 35% ethanol eluate collected above is concentrated and dried under reduced pressure in a vacuum, and then dissolved in 10 times of 75% ethanol.

[0156] (4) Detection of digestion-promoting function: The specific operation and calculation are as above. The pepsin activity of this combination is 24.83 (u), the pepsin activity of the blank group is 11.67 (u), and the promotion rate is 112.76%.

[0157] Compared with Example 1, the sample of Comparative Example 11 is not within the combination range, and the pepsin activity promotion rate drops from 137.44% to 112.76%.

[0158] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition with a function of promoting digestion, characterized in that, By weight parts, it comprises the following components: 4-6 parts of Pueraria flower, 4-6 parts of Cistanche deserticola, 8-10 parts of Sophora japonica flower, 4-6 parts of Fagopyrum dibotrys, and 8-10 parts of Terminalia myriocarpa Hance var. glabra.

2. The traditional Chinese medicine composition according to claim 1, wherein The raw materials consist of the following components: 4-6 parts of Pueraria flower, 4-6 parts of Cistanche deserticola, 8-10 parts of Sophora japonica flower, 4-6 parts of Fagopyrum dibotrys, and 8-10 parts of Terminalia myriocarpa Hance var. glabra.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition is prepared by the steps of extraction, concentration, purification, and concentration and drying of the raw materials.

4. A method for preparing a traditional Chinese medicine composition with a digestive function promoting effect according to any one of claims 1-3, characterized in that, It includes the following steps: (1) Extraction: The raw materials are crushed and extracted with ethanol. (2) Concentration and drying: The filtered extract is concentrated and dried under reduced pressure in a vacuum. (3) Preparation and purification: The extract obtained in step (2) is dissolved in ethanol, adsorbed on an adsorption column, eluted with 10 times of ultrapure water for 1 hour, eluted with 10 times of 35% ethanol for 1 hour, eluted with 10 times of 75% ethanol for 1 hour, eluted with 95% ethanol for 1 hour, and the eluate is collected. (4) Concentration and drying: The collected 75% ethanol and ultrapure water eluate are concentrated and dried under reduced pressure in a vacuum to obtain the product.

5. The method according to claim 4, wherein In step (1), 10 times of 75% ethanol solution is added, and ultrasonic extraction is carried out 2-3 times at 450-500W for 80-90 minutes each time. After the extraction is completed, it is cooled to room temperature, filtered, and the filtrates are combined for standby.

6. The method according to claim 4, wherein In step (2), the vacuum degree of the reduced pressure vacuum concentration is 150 mbar, and the temperature is 45-50 °C to obtain a solid powder.

7. The method according to claim 4, characterized in that, In step (3), the extract is dissolved in 10 times of 75% ethanol and adsorbed on an AB-8 resin column for 2 hours.

8. The method according to claim 4, characterized in that, In step (4), the vacuum degree of the reduced pressure vacuum concentration is 150 mbar, and the temperature is 45-50 °C to obtain a solid powder.

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