Qiqing toxin-vanquishing granular preparation for poultry and preparation method thereof

Abstract

The invention relates to the technical field of pharmaceutical preparations, in particular to a Qiqing toxin-vanquishing granular preparation for poultry and a preparation method of the Qiqing toxin-vanquishing granular preparation. The Qiqing toxin-vanquishing granular preparation for poultry is prepared from the following raw materials in parts by mass: 15 to 30 parts of radix scutellariae, 15 to 30 parts of rhizoma polygoni cuspidati, 10 to 20 parts of radix pulsatillae, 10 to 20 parts of radix sophorae flavescentis, 15 to 30 parts of radix isatidis, 10 to 20 parts of rhizoma dryopteris crassirhizomae, 5 to 15 parts of folium isatidis, 10 to 15 parts of hydroxypropyl-beta-cyclodextrin, 0.1 to 1 part of dispersing agent, 1 to 3 parts of compound enzyme preparation, 1 to 3 parts of astragalus polysaccharide and 70 to 100 parts of saccharose. The invention has no toxic or side effect, successfully solves the industrial problems of slow effect and large dosage of the traditional Chinese veterinary medicine, and provides reliable technical support for antibiotic-free breeding.

Description

Technical Field

[0001] The invention relates to the technical field of pharmaceutical preparations, and in particular to a Qiqing Baidu granule preparation for poultry and a preparation method thereof. Background Art

[0002] The poultry industry has developed from scattered farming in the past to intensive large-scale farming. Currently, it is affected by many factors such as management level, feeding conditions, epidemic prevention level, climate, etc., which provide conditions for the spread of certain diseases. Sick chicks are weak, have no appetite, and are prone to sudden death, causing serious economic losses.

[0003] Due to the widespread use of chemical synthetic veterinary drugs in poultry farming, the problem of veterinary drug residues in animal products has become increasingly prominent. Domestic and foreign research results show that long-term or excessive abuse of veterinary drugs will not only reduce the quality of livestock products and cause significant economic losses, but also endanger human health and seriously endanger life, which is more harmful to humans than animal diseases.

[0004] At present, most Chinese veterinary drugs have become an important type of therapeutic drugs in veterinary clinics due to their low toxicity and side effects and the difficulty of long-term use to produce residues. In particular, Chinese veterinary drug preparations are becoming more and more popular in the production of pollution-free livestock products. However, Chinese medicine preparations generally have low bioavailability and slow drug release. They require large amounts of feeding to achieve the expected effect, resulting in serious drug waste, which needs to be solved urgently. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a Qiqing Baidu granule preparation for poultry and a preparation method thereof.

[0006] A Qiqing Baidu granule preparation for poultry, the raw materials of which include, by weight: 15-30 parts of scutellaria baicalensis, 15-30 parts of polygonum cuspidatum, 10-20 parts of pulsatilla, 10-20 parts of sophora flavescens, 15-30 parts of isatis root, 10-20 parts of cyrtomium mongolicum, 5-15 parts of isatis indigotica, 10-15 parts of hydroxypropyl-β-cyclodextrin, 0.1-1 parts of dispersant, 1-3 parts of complex enzyme preparation, 1-3 parts of astragalus polysaccharide and 70-100 parts of sucrose.

[0007] Scutellaria baicalensis, bitter in taste, cold in nature. Enters the lung, gallbladder, spleen, large intestine, and small intestine meridians. Clears away heat and dampness, purges fire and detoxifies, stops bleeding, and stabilizes the fetus. It is used for damp-heat, chest tightness and nausea due to summer heat, damp-heat fullness, diarrhea, jaundice, lung heat cough, high fever and thirst, blood heat vomiting and bleeding, carbuncle, sore, and fetal movement disorder.

[0008] Polygonum cuspidatum, slightly bitter in taste, slightly cold in nature. Enters the liver, gallbladder, and lung meridians. Clears away heat and detoxifies, promotes bile secretion and relieves jaundice, dispels wind and dampness, disperses blood stasis and relieves pain, relieves cough and resolves phlegm. Used for joint pain, damp-heat jaundice, amenorrhea, dysentery, cough with sputum, burns caused by water or fire, injuries from falls, carbuncle, swelling, and sores.

[0009] Pulsatilla chinensis (Bunge) Regel is bitter in taste and cold in nature. It acts on the stomach meridian and large intestine meridian. It clears heat and cools blood, and detoxifies. It is used for dysentery with heat-toxin in the blood, alternating chills and fever of warm malaria, epistaxis, and hemorrhoids.

[0010] Sophora flavescens Aiton is bitter in taste and cold in nature. It acts on the small intestine meridian, liver meridian, kidney meridian, and large intestine meridian. It clears heat and dries dampness, dispels wind and kills parasites. It is used for damp-heat diarrhea, bloody stool due to intestinal wind, jaundice, difficult urination, edema, leukorrhea, itching of the vulva, scabies, leprosy, skin itching, and sores and ulcers with damp-toxin.

[0011] Isatis indigotica Fortune is bitter in taste and cold in nature. It acts on the stomach meridian and liver meridian. It clears heat and detoxifies, cools blood and relieves sore throat. It is used for epidemic febrile disease with maculae, purple and dark tongue with crimson spots, mumps, sore throat, scarlet fever with rotten throat, epidemic encephalitis B, erysipelas, and carbuncles.

[0012] Dryopteris crassirhizoma Nakai is bitter in taste and slightly cold in nature. It acts on the liver and stomach meridians. It clears heat and detoxifies, expels parasites. It is used for abdominal pain due to worm accumulation and sores and ulcers. Dryopteris crassirhizoma Nakai carbonized is used to stop bleeding and is used for metrorrhagia and metrostaxis.

[0013] Isatis tinctoria L. is bitter in taste and cold in nature. It acts on the stomach meridian and heart meridian. It clears heat and detoxifies, cools blood and eliminates maculae. It is used for pathogenic warm factors entering the nutrient system, high fever with coma, maculae and eruptions, jaundice, dysentery with heat, mumps, sore throat, erysipelas, and carbuncles.

[0014] Preferably, the dispersant is polyvinylpyrrolidone.

[0015] Preferably, the complex enzyme preparation includes: cellulase, pectinase.

[0016] More preferably, the specific activity of cellulase is 3000 - 6000 U / g, and the specific activity of pectinase is 2000 - 4000 U / g.

[0017] The preparation method of the above-mentioned Qiqing Baidu Keli preparation for poultry includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to the mixture of chlorosulfonic acid and dichloroethane, stir in an ice-salt bath for 6 - 8 h, neutralize to neutral, dialyze, remove the solvent, and freeze-dry; then add the dispersant and water and mix, and perform cyclic homogenization treatment 2 - 4 times to obtain pretreated cyclodextrin; S2. Mix Scutellaria baicalensis Georgi, Polygonum cuspidatum Sieb. et Zucc., Pulsatilla chinensis (Bunge) Regel, Sophora flavescens Aiton, Isatis indigotica Fortune, Dryopteris crassirhizoma Nakai, and Isatis tinctoria L. evenly, freeze and crush to a particle size ≤50 μm, add the complex enzyme preparation, astragalus polysaccharide, and water and mix, adjust the pH value of the system to 4.5 - 5, enzymatically hydrolyze at 45 - 50 °C for 1 - 2 h, soak in water for 1 - 2 h, extract at high temperature for 1 - 3 h, and filter; concentrate the filtrate under reduced pressure to obtain a clear extract; S3. Mix the clear extract and the pretreated cyclodextrin, stir at 50 - 60 °C for 1 - 2 h, cool to 33 - 37 °C and keep warm for 20 - 40 min, stir with sucrose for 20 - 30 min, granulate, and dry.

[0018] Preferably, in S1, the homogenization pressure is 30 - 40 MPa.

[0019] Preferably, in S2, the filtrate is concentrated under reduced pressure to a relative density of 1.20 - 1.25.

[0020] Preferably, in S2, the temperature for concentration under reduced pressure is 50 - 60 °C, and the vacuum degree is -0.06 to -0.09 MPa.

[0021] Preferably, in S3, after granulation, it is sieved through a 24 - mesh sieve.

[0022] Preferably, in S3, it is dried until the moisture content ≤ 6%, and the drying temperature is 60 - 65 °C.

[0023] Beneficial effects: The Qiging Baidu Granule preparation obtained by the present invention realizes the modernization upgrade of traditional Chinese veterinary medicine through innovation, and has significant advantages in preventing and treating common poultry diseases such as pullorum disease and damp - heat diarrhea in chicks.

[0024] The present invention adopts liquid nitrogen cryogenic grinding combined with complex enzymatic hydrolysis technology to generate micro - cracks in the cell walls of medicinal materials, effectively promoting the dissolution of fat - soluble components such as polydatin and matrine; hydroxypropyl - β - cyclodextrin not only greatly improves water - solubility, but also has a good inclusion effect on fat - soluble drugs in its internal cavity. At the same time, after sulfonation treatment, by introducing sulfonic acid group negative charges, the binding force with basic drugs is enhanced through electrostatic interaction, which not only shortens the time to reach the peak blood drug concentration, but also can effectively extend the effective concentration, effectively controlling the release and reducing drug waste.

[0025] The present invention is only made of Chinese medicinal materials without antibiotics, which can promote the chicks after cure to eat and their development to return to normal, ensure their subsequent growth and development, and avoid problems such as anorexia, slow growth rate and weight loss of chicks after treatment, contributing to the promotion of green and antibiotic - free breeding, and having a significant effect compared with existing drugs.

[0026] The present invention has no any toxic and side effects, and successfully solves the industry problems of slow onset and large dosage of traditional Chinese veterinary medicine, providing reliable technical support for antibiotic - free breeding. Description of the drawings

[0027] Figure 1 It is a comparison chart of the antibacterial diameters of the Flos Chrysanthemi preparations obtained in Example 5 and Comparative Examples 1 - 2 against test bacteria (Staphylococcus aureus, Bacillus dysenteriae, Pseudomonas aeruginosa, Escherichia coli, Proteus).

[0028] Figure 2 It is a comparison chart of the body weights, duodenal bacterial loads and cecal bacterial loads of chickens in the blank group, model group, Example 5 group, Comparative Example 1 group, Comparative Example 2 group and amoxicillin group. Detailed implementation manners

[0029] The present invention will be further explained below in combination with specific embodiments.

[0030] Example 1 ‌A Qiging Baidu Granule preparation for poultry, the raw materials of which include: 150 g of Scutellaria baicalensis, 150 g of Polygonum cuspidatum, 100 g of Pulsatilla chinensis, 100 g of Sophora flavescens, 150 g of Isatis indigotica, 100 g of Dryopteris crassirhizoma, 50 g of Folium Isatidis, 100 g of hydroxypropyl-β-cyclodextrin, 1 g of polyvinylpyrrolidone, 10 g of complex enzyme preparation, 10 g of astragalus polysaccharide, and 700 g of sucrose.

[0031] The complex enzyme preparation is composed of cellulase and pectinase; among them, the specific activity of cellulase is 3000 U / g, and the specific activity of pectinase is 2000 U / g.

[0032] The preparation method of the above Qiging Baidu Granule preparation for poultry includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to the mixture of chlorosulfonic acid and dichloroethane. The weight ratio of hydroxypropyl-β-cyclodextrin to chlorosulfonic acid and dichloroethane is 100:25:250. Stir in an ice-salt bath for 7 h, slowly add ice water to quench, neutralize to neutral with sodium bicarbonate, use a dialysis bag to dialyze to remove small molecule impurities, remove the solvent, and freeze-dry; then add polyvinylpyrrolidone and 100 g of water and mix, send it to a high-pressure homogenizer for 2 cycles, and the homogenization pressure is 30 MPa to obtain pretreated cyclodextrin; S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Folium Isatidis evenly, and use liquid nitrogen to freeze and crush to a particle size of ≤50 μm. Add the complex enzyme preparation, astragalus polysaccharide, and 2000 g of water and mix. Adjust the pH value of the system to 4.5 - 5, enzymatically hydrolyze at 45 °C for 1 h, add it to an extraction tank, add water to cover the traditional Chinese medicine, soak for 1 h, maintain at 80 °C for 1 h, and filter the decocted medicinal liquid through a 200-mesh filter; concentrate the filtrate under reduced pressure to a relative density of 1.20, control the reduced pressure concentration temperature at 50 °C, and the vacuum degree at -0.06 MPa to obtain clear paste; S3. Mix the clear paste and pretreated cyclodextrin, stir at 50 °C for 1 h, the stirring speed is 100 r / min, cool to 33 °C and keep warm for 20 min, and stir with sucrose in a trough mixer for 20 min to make soft material. Add it to a swing granulator, pass through a 24-mesh sieve to make wet granules, put the wet granules into a baking tray, and dry at 60 °C until the moisture content is ≤6%.

[0033] Example 2 ‌A Qiging Baidu Granule preparation for poultry, the raw materials of which include: 300 g of Scutellaria baicalensis, 300 g of Polygonum cuspidatum, 200 g of Pulsatilla chinensis, 200 g of Sophora flavescens, 300 g of Isatis indigotica, 200 g of Dryopteris crassirhizoma, 150 g of Folium Isatidis, 150 g of hydroxypropyl-β-cyclodextrin, 10 g of polyvinylpyrrolidone, 30 g of complex enzyme preparation, 30 g of astragalus polysaccharide, and 1000 g of sucrose.

[0034] The complex enzyme preparation consists of cellulase and pectinase; among them, the specific activity of cellulase is 6000 U / g, and the specific activity of pectinase is 4000 U / g.

[0035] The preparation method of the above-mentioned Septem-Qingbai-Du Granule preparation for poultry includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to the mixture of chlorosulfonic acid and dichloroethane. The weight ratio of hydroxypropyl-β-cyclodextrin to chlorosulfonic acid and dichloroethane is 150:20:200. Stir in an ice-salt bath for 8 h, slowly add ice water to quench, neutralize to neutral with sodium bicarbonate, dialyze with a dialysis bag to remove small molecule impurities, remove the solvent, and freeze-dry; then add polyvinylpyrrolidone and 300 g of water and mix, and send it to a high-pressure homogenizer for 4 cycles. The homogenization pressure is 40 MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis tinctoria, Dryopteris crassirhizoma, and Folium Isatidis evenly, freeze and crush them with liquid nitrogen to a particle size ≤ 50 μm, add the complex enzyme preparation, astragalus polysaccharide, and 4000 g of water and mix. Adjust the pH value of the system to 4.5 - 5, enzymatically hydrolyze at 50 °C for 2 h, add it to an extraction tank, add water to cover the traditional Chinese medicine, soak for 2 h, maintain at 90 °C for 3 h, and filter the decocted medicinal liquid through a 200-mesh filter screen; concentrate the filtrate under reduced pressure to a relative density of 1.25. The reduced pressure concentration temperature is controlled at 60 °C, and the vacuum degree is -0.07 MPa to obtain clear paste. S3. Mix the clear paste and the pretreated cyclodextrin, stir at 60 °C for 2 h, the stirring speed is 300 r / min, cool to 37 °C and keep warm for 40 min, and stir with sucrose in a trough mixer for 30 min to make soft materials. Add them to a swing granulator, pass through a 24-mesh sieve to make wet granules, put the wet granules into a baking tray, and dry at 65 °C until the moisture content ≤ 6%.

[0036] Example 3 ‌A Septem-Qingbai-Du Granule preparation for poultry, whose raw materials include: 200 g of Scutellaria baicalensis, 240 g of Polygonum cuspidatum, 120 g of Pulsatilla chinensis, 160 g of Sophora flavescens, 200 g of Isatis tinctoria, 180 g of Dryopteris crassirhizoma, 80 g of Folium Isatidis, 130 g of hydroxypropyl-β-cyclodextrin, 7 g of polyvinylpyrrolidone, 15 g of complex enzyme preparation, 25 g of astragalus polysaccharide, and 800 g of sucrose.

[0037] The complex enzyme preparation consists of cellulase and pectinase; among them, the specific activity of cellulase is 5000 U / g, and the specific activity of pectinase is 2500 U / g.

[0038] The preparation method of the above-mentioned Septem-Qingbai-Du Granule preparation for poultry includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to the mixture of chlorosulfonic acid and dichloroethane. The weight ratio of hydroxypropyl-β-cyclodextrin to chlorosulfonic acid and dichloroethane is 130:20:200. Stir in an ice-salt bath for 6 h, slowly add ice water dropwise to quench, neutralize to neutral with sodium bicarbonate, use a dialysis bag to dialyze to remove small molecule impurities, remove the solvent, and freeze-dry; then add polyvinylpyrrolidone and 250 g of water and mix, send it to a high-pressure homogenizer for 3 cycles of treatment, and the homogenization pressure is 33 MPa to obtain pretreated cyclodextrin; S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis tinctoria, Dryopteris crassirhizoma, and Isatis indigotica Fort. evenly, use liquid nitrogen to freeze and crush to a particle size ≤ 50 μm, add a compound enzyme preparation, astragalus polysaccharide, and 3400 g of water and mix, adjust the pH value of the system to 4.5 - 5, enzymatically hydrolyze at 46 °C for 100 min, add it to an extraction tank, add water to cover the traditional Chinese medicine, soak for 80 min, maintain at 85 °C for 2.5 h, and filter the decocted medicinal liquid through a 200-mesh filter; concentrate the filtrate under reduced pressure to a relative density of 1.21, control the temperature of reduced pressure concentration at 58 °C, and the vacuum degree at -0.08 MPa to obtain clear paste; S3. Mix the clear paste and pretreated cyclodextrin, stir at 58 °C for 80 min, the stirring speed is 250 r / min, cool to 33 °C and keep warm for 35 min, add sucrose to a trough mixer and stir for 22 min to make soft material, add it to a rocking granulator, pass through a 24-mesh sieve to make wet granules, put the wet granules into a baking tray, and dry at 63 °C until the moisture content ≤ 6%.

[0039] Example 4 ‌A seven-clearing and detoxifying granule preparation for poultry, the raw materials of which include: 240 g of Scutellaria baicalensis, 200 g of Polygonum cuspidatum, 180 g of Pulsatilla chinensis, 120 g of Sophora flavescens, 280 g of Isatis tinctoria, 140 g of Dryopteris crassirhizoma, 120 g of Isatis indigotica Fort., 110 g of hydroxypropyl-β-cyclodextrin, 3 g of polyvinylpyrrolidone, 25 g of compound enzyme preparation, 15 g of astragalus polysaccharide, and 900 g of sucrose.

[0040] The compound enzyme preparation is composed of cellulase and pectinase; among them, the specific activity of cellulase is 4000 U / g, and the specific activity of pectinase is 3500 U / g.

[0041] The preparation method of the above-mentioned seven-clearing and detoxifying granule preparation for poultry includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to the mixture of chlorosulfonic acid and dichloroethane. The weight ratio of hydroxypropyl-β-cyclodextrin to chlorosulfonic acid and dichloroethane is 110:20:200. Stir in an ice-salt bath for 6 h, slowly add ice water dropwise to quench, neutralize to neutral with sodium bicarbonate, use a dialysis bag to dialyze to remove small molecule impurities, remove the solvent, and freeze-dry; then add polyvinylpyrrolidone and 150 g of water and mix, send it to a high-pressure homogenizer for 3 cycles of treatment, and the homogenization pressure is 37 MPa to obtain pretreated cyclodextrin; S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria evenly, and freeze-grind them to a particle size of ≤50 μm using liquid nitrogen. Add a composite enzyme preparation, astragalus polysaccharide, and 2600 g of water, mix well, adjust the pH value of the system to 4.5 - 5, enzymatically hydrolyze at 48°C for 80 min, add the mixture to an extraction tank, add water to submerge the traditional Chinese medicine, soak for 100 min, maintain at 85°C for 1.5 h, and filter the decocted liquid through a 200-mesh filter screen; concentrate the filtrate under reduced pressure to a relative density of 1.23, control the reduced pressure concentration temperature at 55°C, and the vacuum degree at -0.085 MPa to obtain a clear extract. S3. Mix the clear extract and pretreated cyclodextrin, stir at 52°C for 100 min at a stirring speed of 150 r / min, cool to 37°C and keep warm for 25 min, add sucrose and stir in a trough mixer for 28 min to make a soft material. Add the soft material to a swing granulator, pass through a 24-mesh sieve to make wet granules, place the wet granules in a baking tray, and dry at 61°C until the moisture content is ≤6%.

[0042] Example 5 A seven-clearing and detoxifying granule preparation for poultry, the raw materials of which include: 220 g of Scutellaria baicalensis, 220 g of Polygonum cuspidatum, 150 g of Pulsatilla chinensis, 150 g of Sophora flavescens, 240 g of Isatis indigotica, 150 g of Dryopteris crassirhizoma, 100 g of Isatis tinctoria, 120 g of hydroxypropyl-β-cyclodextrin, 5 g of polyvinylpyrrolidone, 20 g of a composite enzyme preparation, 20 g of astragalus polysaccharide, and 850 g of sucrose.

[0043] The composite enzyme preparation is composed of cellulase and pectinase; among them, the specific activity of cellulase is 4500 U / g, and the specific activity of pectinase is 3000 U / g.

[0044] The preparation method of the above seven-clearing and detoxifying granule preparation for poultry includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to a mixture of chlorosulfonic acid and dichloroethane. The weight ratio of hydroxypropyl-β-cyclodextrin to chlorosulfonic acid and dichloroethane is 120:20:200. Stir in an ice-salt bath for 7 h, slowly add ice water to quench, neutralize to neutral with sodium bicarbonate, dialyze using a dialysis bag to remove small molecule impurities, remove the solvent, and freeze-dry; then add polyvinylpyrrolidone and 200 g of water, mix well, and send it to a high-pressure homogenizer for 3 cycles of treatment. The homogenization pressure is 35 MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria evenly, and freeze and pulverize them with liquid nitrogen to a particle size of ≤50 μm. Add a compound enzyme preparation, Astragalus polysaccharide, and 3000 g of water, mix well, adjust the pH value of the system to 4.5 - 5, enzymatically hydrolyze at 47°C for 90 min, add it to an extraction tank, add water to cover the Chinese medicine, soak for 90 min, maintain at 90°C for 2 h, and filter the decocted liquid through a 200-mesh filter screen; Concentrate the filtrate under reduced pressure to a relative density of 1.22, control the temperature of reduced pressure concentration at 55°C, and the vacuum degree at -0.085 MPa to obtain a clear extract; S3. Mix the clear extract and pretreated cyclodextrin, stir at 55°C for 90 min, with a stirring speed of 200 r / min, cool to 35°C and keep warm for 30 min, add sucrose and stir in a trough mixer for 25 min to make a soft material, add it to a shaking granulator, pass through a 24-mesh sieve to make wet granules, put the wet granules into a baking tray, and dry at 62°C until the moisture content is ≤6%.

[0045] Comparative Example 1 A preparation of Qiqing Baidu granules for poultry, the raw materials of which include: 220 g of Scutellaria baicalensis, 220 g of Polygonum cuspidatum, 150 g of Pulsatilla chinensis, 150 g of Sophora flavescens, 240 g of Isatis indigotica, 150 g of Dryopteris crassirhizoma, 100 g of Isatis tinctoria, 120 g of hydroxypropyl-β-cyclodextrin, 5 g of polyvinylpyrrolidone, 20 g of a compound enzyme preparation, 20 g of Astragalus polysaccharide, and 850 g of sucrose.

[0046] The compound enzyme preparation is composed of cellulase and pectinase; among them, the specific activity of cellulase is 4500 U / g, and the specific activity of pectinase is 3000 U / g.

[0047] The preparation method of the above-mentioned Qiqing Baidu granules preparation for poultry includes the following steps: S1. Mix hydroxypropyl-β-cyclodextrin, polyvinylpyrrolidone, and 200 g of water, send it into a high-pressure homogenizer for 3 cycles of treatment, with a homogenization pressure of 35 MPa, to obtain pretreated cyclodextrin; S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria evenly, and freeze and pulverize them with liquid nitrogen to a particle size of ≤50 μm. Add a compound enzyme preparation, Astragalus polysaccharide, and 3000 g of water, mix well, adjust the pH value of the system to 4.5 - 5, enzymatically hydrolyze at 47°C for 90 min, add it to an extraction tank, add water to cover the Chinese medicine, soak for 90 min, maintain at 90°C for 2 h, and filter the decocted liquid through a 200-mesh filter screen; Concentrate the filtrate under reduced pressure to a relative density of 1.22, control the temperature of reduced pressure concentration at 55°C, and the vacuum degree at -0.085 MPa to obtain a clear extract; S3. Mix the clear extract and the pretreated cyclodextrin, stir at 55°C for 90 min at a stirring speed of 200 r / min, cool to 35°C and keep warm for 30 min, add sucrose and stir in a trough mixer for 25 min to make soft materials, add them to a rocking granulator, pass through a 24-mesh sieve to make wet granules, put the wet granules into a baking tray, and dry at 62°C until the moisture content is ≤6%.

[0048] Comparative Example 2 A seven-herb detoxifying granule preparation for poultry, the raw materials of which include: 220 g of Scutellaria baicalensis, 220 g of Lonicera japonica, 150 g of Pulsatilla chinensis, 150 g of Sophora flavescens, 240 g of Isatis indigotica, 150 g of Dryopteris crassirhizoma, 100 g of Folium Isatidis, 120 g of hydroxypropyl-β-cyclodextrin, 5 g of polyvinylpyrrolidone, 20 g of complex enzyme preparation, 20 g of astragalus polysaccharide, and 850 g of sucrose.

[0049] The complex enzyme preparation is composed of cellulase and pectinase; among them, the specific activity of cellulase is 4500 U / g, and the specific activity of pectinase is 3000 U / g.

[0050] The preparation method of the above-mentioned seven-herb detoxifying granule preparation for poultry includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to the mixture of chlorosulfonic acid and dichloroethane, the weight ratio of hydroxypropyl-β-cyclodextrin to chlorosulfonic acid and dichloroethane is 120:20:200, stir in an ice-salt bath for 7 h, slowly add ice water to quench, neutralize to neutral with sodium bicarbonate, use a dialysis bag to dialyze to remove small molecule impurities, remove the solvent, and freeze-dry; then add polyvinylpyrrolidone and 200 g of water and mix, send to a high-pressure homogenizer for 3 cycles, and the homogenization pressure is 35 MPa to obtain pretreated cyclodextrin; S2. Mix Scutellaria baicalensis, Lonicera japonica, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Folium Isatidis evenly, freeze and crush them with liquid nitrogen to a particle size of ≤50 μm, add the complex enzyme preparation, astragalus polysaccharide, and 3000 g of water and mix, adjust the pH value of the system to 4.5 - 5, enzymatically hydrolyze at 47°C for 90 min, add to an extraction tank, add water to cover the traditional Chinese medicine, soak for 90 min, maintain at 90°C for 2 h, and filter the decocted medicinal liquid through a 200-mesh filter screen; concentrate the filtrate under reduced pressure to a relative density of 1.22, control the temperature of reduced pressure concentration at 55°C, and the vacuum degree at -0.085 MPa to obtain a clear extract; S3. Mix the clear extract and the pretreated cyclodextrin, stir at 55°C for 90 min at a stirring speed of 200 r / min, cool to 35°C and keep warm for 30 min, add sucrose and stir in a trough mixer for 25 min to make soft materials, add them to a rocking granulator, pass through a 24-mesh sieve to make wet granules, put the wet granules into a baking tray, and dry at 62°C until the moisture content is ≤6%.

[0051] The in vitro antibacterial tests were carried out on the particle preparations obtained in Example 5 and Comparative Examples 1-2 as follows: After inoculating Staphylococcus aureus, Shigella dysenteriae, Pseudomonas aeruginosa, Escherichia coli, and Proteus vulgaris respectively, holes with a diameter of 5 mm were punched on each agar plate. The seven-clear and toxin-detoxifying particle preparations obtained in Example 5 and Comparative Examples 1-2 were diluted 100 times respectively and then added into the holes of each agar plate. They were cultured in an incubator at 37 °C for 24 h, and the antibacterial diameters of the drugs on each agar plate in each group were counted.

[0052] As Figure 1 shown, the seven-clear and toxin-detoxifying particle preparations obtained in Example 5 and Comparative Examples 1-2 had antibacterial effects on Staphylococcus aureus, Shigella dysenteriae, Pseudomonas aeruginosa, Escherichia coli, and Proteus vulgaris to varying degrees; and the antibacterial effect of the seven-clear and toxin-detoxifying particle preparation obtained in Example 5 on each strain was the strongest, superior to that of Comparative Examples 1-2 (P < 0.05).

[0053] Animal tests were carried out on the yellow flower preparations obtained in Example 5 and Comparative Examples 1-2. Hy-Line Brown laying hens were used as the experimental objects. 180 one-day-old Hy-Line Brown laying hens were taken and raised until 7 days old with good growth conditions. They were randomly divided into 6 groups, namely the blank group, the model group, the Example 5 group, the Comparative Example 1 group, the Comparative Example 2 group, and the amoxicillin group. Each hen in the blank group was intragastrically administered 0.2 mL of normal saline, and each hen in the remaining groups was intragastrically administered 0.2 mL of bacterial solution (concentration: 2.5×10 8 CFU / 0.2 mL) for modeling. Subsequently, the Example 5 group, the Comparative Example 1 group, and the Comparative Example 2 group were intragastrically administered drugs (5 g of the product was added to every 1 L of water), the amoxicillin group was intragastrically administered amoxicillin soluble powder (the administration dose was 0.4 g / kg), and the model group was intragastrically administered an equal amount of distilled water. Each group was administered once a day for a total of 5 days; during the test period, each group had free access to food and water. After stopping the drug, they were observed for another 5 days. The chickens in each group were weighed, and then sacrificed and dissected. The duodenal contents and cecal contents were taken to detect their bacterial loads (assuming that the bacterial loads of the blank group were all 0).

[0054] As Figure 2 shown, the blank group had the highest body weight, with no significant difference from the Example 5 group, and the body weights of the blank group and the Example 5 group were superior to those of the remaining groups (P < 0.05); the amoxicillin group had the lowest duodenal bacterial load, with no significant difference from the Example 5 group, and the duodenal bacterial loads of the amoxicillin group and the Example 5 group were superior to those of the remaining groups (P < 0.05); the Example 5 group had the lowest cecal bacterial load, superior to the remaining groups (P < 0.05).

[0055] As mentioned above, the above are only the preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, makes equivalent substitutions or changes, and all should be covered by the protection scope of the present invention.

Claims

1. A Septem-Clearing and Toxin-Removing Granule Preparation for Poultry, characterized in that, Its raw materials by mass parts include: 15 - 30 parts of Scutellaria baicalensis, 15 - 30 parts of Polygonum cuspidatum, 10 - 20 parts of Pulsatilla chinensis, 10 - 20 parts of Sophora flavescens, 15 - 30 parts of Isatis indigotica, 10 - 20 parts of Dryopteris crassirhizoma, 5 - 15 parts of Folium Isatidis, 10 - 15 parts of hydroxypropyl-β-cyclodextrin, 0.1 - 1 part of dispersant, 1 - 3 parts of compound enzyme preparation, 1 - 3 parts of astragalus polysaccharide, and 70 - 100 parts of sucrose.

2. The seven-clearing and toxin-detoxifying granule preparation for poultry according to claim 1, wherein The dispersant is polyvinylpyrrolidone.

3. The seven-clearing and toxin-detoxifying granule preparation for poultry according to claim 1, wherein The compound enzyme preparation includes: cellulase, pectinase.

4. The seven-clearing and toxin-eliminating granule preparation for poultry according to claim 3, wherein, The specific activity of cellulase is 3000 - 6000 U / g, and the specific activity of pectinase is 2000 - 4000 U / g.

5. A preparation method of the seven-herb anti-toxic granule preparation for poultry according to any one of claims 1-4, characterized in that, It includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin into the mixture of chlorosulfonic acid and dichloroethane, stir in an ice-salt bath for 6 - 8 h, neutralize to neutral, dialyze, remove the solvent, and freeze-dry; then add the dispersant and water and mix, and perform cyclic homogenization treatment 2 - 4 times to obtain pretreated cyclodextrin; S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Folium Isatidis evenly, freeze and crush to a particle size ≤ 50 μm, add the compound enzyme preparation, astragalus polysaccharide, and water and mix, adjust the pH value of the system to 4.5 - 5, enzymolyze at 45 - 50 °C for 1 - 2 h, soak in water for 1 - 2 h, extract for 1 - 3 h, and filter; concentrate the filtrate under reduced pressure to obtain a clear paste; S3. Mix the clear paste and the pretreated cyclodextrin, stir at 50 - 60 °C for 1 - 2 h, cool to 33 - 37 °C and keep warm for 20 - 40 min, stir with sucrose for 20 - 30 min, granulate, and dry.

6. The preparation method of the seven-clearing and toxin-removing granule preparation for poultry according to claim 5, wherein, In S1, the homogenization pressure is 30 - 40 MPa.

7. The preparation method of the seven-clearing and toxin-removing granule preparation for poultry according to claim 5, characterized in that, In S2, the filtrate is concentrated under reduced pressure to a relative density of 1.20 - 1.

25.

8. The preparation method of the seven-clearing and toxicity-detoxifying granule preparation for poultry according to claim 5, wherein In S2, the temperature for concentration under reduced pressure is 50 - 60 °C, and the vacuum degree is -0.06 to -0.09 MPa.

9. The preparation method of the seven-clearing and toxicity-detoxifying granule preparation for poultry according to claim 5, characterized in that, In S3, after granulation, pass through a 24-mesh sieve.

10. The preparation method of the seven-clearing and toxin-detoxifying granule preparation for poultry according to claim 5, characterized in that, In S3, dry until the water content ≤ 6%, and the drying temperature is 60 - 65 °C.

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