Compound preparation for preventing and treating bacterial diseases of livestock and poultry and preparation method thereof

Abstract

The invention discloses a compound preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method of the compound preparation. The compound preparation is prepared from the following components in parts by weight: 3.2 to 5.8 parts of astragalus polysaccharide powder, 2.9 to 5.3 parts of xylooligosaccharide powder, 2.5 to 4.8 parts of compound enzyme agent, 2 to 4.4 parts of compound bacterium agent, 1.5 to 3.6 parts of selenium yeast powder, 1.3 to 3.2 parts of egg yolk antibody, 1 to 2.1 parts of hydroxypropyl methyl cellulose, 0.35 to 0.8 part of chitosan and 0.3 to 0.65 part of montmorillonite. The compound preparation disclosed by the invention can effectively inhibit growth and reproduction of viruses such as riemerella anatipestifer, escherichia coli and salmonella, and is beneficial to regulation of immune functions of animals and improvement of body resistance of the animals.

Description

Technical Field

[0001] The present invention relates to a composite preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method thereof, belonging to the technical field of video playback. Background Art

[0002] In the practice of large-scale livestock and poultry breeding, the breeding mode is continuously optimized, such as the standardized three-dimensional breeding mode, the fermentation bed breeding mode, etc. Although the breeding efficiency is improved to a certain extent and the occurrence of diseases is reduced, there are still problems such as large livestock and poultry stocking density, wet indoor ground, poor ventilation, and poor atmospheric environment, leading to the frequent occurrence of bacterial diseases in livestock and poultry, such as Riemerella anatipestifer disease, Escherichia coli disease, Salmonella disease, etc.

[0003] In the traditional breeding process, antibiotics are often added to the feed to prevent and treat diseases and promote growth. However, long-term use of antibiotics will cause the imbalance of the intestinal flora of livestock and poultry, enhance the drug resistance of pathogenic bacteria, and the use of antibiotics will cause environmental pollution and pose a threat to the health of animals and even humans. Therefore, it is of great practical significance to explore a biological composite preparation that can effectively inhibit harmful bacteria to replace antibiotics. Summary of the Invention

[0004] At least aiming at one of the above problems existing in the prior art, the present invention provides a composite preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method thereof, which can effectively inhibit the growth and reproduction of viruses such as Riemerella anatipestifer, Escherichia coli, and Salmonella, help regulate the immune function of animals themselves, and improve the body resistance of animals.

[0005] In order to achieve the above object, the present invention adopts the following technical scheme: A composite preparation for preventing and treating bacterial diseases of livestock and poultry, comprising the following components and their parts by weight: 3.2 - 5.8 parts of astragalus polysaccharide powder, 2.9 - 5.3 parts of xylo-oligosaccharide powder, 2.5 - 4.8 parts of composite enzyme agent, 2 - 4.4 parts of composite bacteria agent, 1.5 - 3.6 parts of yeast selenium powder, 1.3 - 3.2 parts of egg yolk antibody, 1 - 2.1 parts of hydroxypropyl methylcellulose, 0.35 - 0.8 part of chitosan, and 0.3 - 0.65 part of montmorillonite.

[0006] Preferably, the composite enzyme agent is composed of plant enzyme, mannanase, and cellulase.

[0007] Preferably, the composite enzyme agent is composed of plant enzyme, mannanase, and cellulase in a mass ratio of 1:0.8 - 1:0.3 - 0.6.

[0008] Preferably, the composite enzyme agent is composed of plant enzyme, mannanase, and cellulase in a mass ratio of 1:0.85:0.55.

[0009] Preferably, the enzyme activity units of the plant enzyme, mannanase and cellulase are all ≥50,000 IU / g.

[0010] Preferably, the compound bacterium agent is composed of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus brittanicus.

[0011] Preferably, the compound bacterium agent is composed of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus brittanicus in a mass ratio of 1.3 - 2.5:1.2 - 2:1.

[0012] Preferably, the compound bacterium agent is composed of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus brittanicus in a mass ratio of 1.85:1.5:1.

[0013] Preferably, the viable count of Bacillus subtilis is ≥2.5×10 11 CFU / g.

[0014] Preferably, the viable count of Lactobacillus plantarum is ≥2×10 11 CFU / g.

[0015] Preferably, the viable count of Lactobacillus brittanicus is ≥2.5×10 11 CFU / g.

[0016] The present invention also provides a preparation method of a compound preparation for preventing and treating bacterial diseases of livestock and poultry, comprising the following steps:

[0017] (1) Dissolve hydroxypropyl methylcellulose in a glycerol aqueous solution to form a hydroxypropyl methylcellulose solution, and then add montmorillonite, and perform high-pressure homogenization to obtain a uniformly dispersed mixed suspension;

[0018] (2) Dissolve chitosan in a glycerol aqueous solution to form a chitosan solution, and then add astragalus polysaccharide powder and xylooligosaccharide powder, and stir evenly to obtain a uniform mixed solution;

[0019] (3) Add a compound enzyme agent, a compound bacterium agent, yolk antibody and yeast selenium powder to the above mixed solution, and stir and disperse evenly to obtain a uniformly mixed emulsion;

[0020] (4) Slowly add the mixed suspension while stirring the mixed emulsion, and then keep stirring at a temperature of 25 - 35°C for 1 - 2 h, and then perform freeze-drying to obtain the compound preparation.

[0021] Preferably, the concentration of hydroxypropyl methylcellulose in the hydroxypropyl methylcellulose solution is 3.8 - 4.3 wt%.

[0022] Preferably, the concentration of chitosan in the chitosan solution is 1.4 - 1.7 wt%.

[0023] Preferably, the concentration of glycerol in the glycerol aqueous solution is 25-35 wt%.

[0024] Preferably, the lyophilization conditions are as follows: pre-cooling treatment at -5 to 0 °C for 1 to 1.5 h, then cooling down to -50 to -42 °C and treating for 8 to 10 h, then heating up to -25 to -18 °C and treating for 2 to 3 h, and finally heating up to room temperature.

[0025] The composite preparation of the present invention can be used alone or as a feed additive for animal feed or drinking water, and has the effects of improving the immunity of animals and preventing bacterial diseases. The animals include but are not limited to ducks, chickens, geese, pigs, etc.

[0026] The beneficial effects of the present invention are as follows: 1. The composite preparation of the present invention is formulated with astragalus polysaccharide powder, xylo-oligosaccharide powder, compound enzyme preparation, compound bacterial preparation, yeast selenium powder, and egg yolk antibody, which can effectively inhibit the growth and reproduction of viruses such as Riemerella anatipestifer, Escherichia coli, and Salmonella, and help regulate the immune function of animals themselves and improve the body resistance of animals; 2. The composite preparation for preventing and treating bacterial diseases of livestock and poultry in the present invention can inhibit the reproduction of harmful bacteria in its overall formulation, revive and proliferate in the animal intestine, and exert probiotic properties, which is beneficial to improving the animal intestinal flora, maintaining intestinal health, and also playing an immune regulation role to enhance the body immunity, thereby promoting the growth and development of the body; 3. The preparation method of the composite preparation for preventing and treating bacterial diseases of livestock and poultry in the present invention is a biological drug manufacturing method, which not only ensures that the active ingredients are not damaged during the processing of the product, but also improves the stability of the product during the processing. Moreover, the composite preparation can be used alone or as a feed additive. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is the 24-hour growth effect diagram of harmful bacteria of the control group of the present invention;

[0028] Figure 2 It is the 24-hour growth effect comparison diagram of harmful bacteria of Example 1 of the present invention;

[0029] Figure 3 It is the 24-hour growth effect comparison diagram of harmful bacteria of Example 2 of the present invention;

[0030] Figure 4 It is the 24-hour growth effect comparison diagram of harmful bacteria of Example 3 of the present invention;

[0031] Figure 5 It is the 24-hour growth effect comparison diagram of harmful bacteria of Example 4 of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. The described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention. For those conditions not specified in the embodiments, they shall be carried out according to the conventional conditions or the conditions recommended by the manufacturer.

[0033] The Bacillus subtilis (strain number: CICC 21279), Lactobacillus plantarum (strain number: CICC 20871), and Lactobacillus delbrueckii (strain number: CICC 6080) of the present invention were all purchased from the China Center for Industrial Culture Collection.

[0034] Example 1

[0035] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, the specific steps of the preparation method are as follows:

[0036] Step 1: Mix phytase with an activity unit number of ≥50,000 IU / g, mannanase with an activity unit number of ≥50,000 IU / g, and cellulase with an activity unit number of ≥50,000 IU / g according to a mass ratio of 1:0.85:0.55 to obtain a compound enzyme agent for standby; mix Bacillus subtilis with a viable count of ≥2.5×10 11 CFU / g, Lactobacillus plantarum with a viable count of ≥2×10 11 CFU / g, and Lactobacillus delbrueckii with a viable count of ≥2.5×10 11 CFU / g according to a mass ratio of 1.85:1.5:1 to obtain a compound bacterial agent for standby;

[0037] Step 2: Dissolve 1 part of hydroxypropyl methylcellulose in a 28wt% glycerol aqueous solution to form a 4wt% hydroxypropyl methylcellulose solution, and then add 0.3 part of montmorillonite, and after high-pressure homogenization, obtain a uniformly dispersed mixed suspension;

[0038] Step 3: Dissolve 0.35 part of chitosan in a 28wt% glycerol aqueous solution to form a 1.55wt% chitosan solution, and then add 3.2 parts of astragalus polysaccharide powder and 2.9 parts of xylooligosaccharide powder, and stir evenly to obtain a uniform mixed solution;

[0039] Step 4: Add 2.5 parts of the above compound enzyme agent, 2 parts of the above compound bacterial agent, 1.3 parts of egg yolk antibody, and 1.5 parts of yeast selenium powder to the above mixed solution, and stir and disperse evenly to obtain a uniformly mixed emulsion;

[0040] Step 5: Then, while stirring and mixing the emulsion, slowly add the mixed suspension, and then stir for 1.5 h while maintaining the temperature at 30°C, followed by freeze-drying: first pre-cool at -4°C for 75 min, then cool down to -45°C and treat for 9 h, then warm up to -22°C and treat for 2.5 h, and finally warm up to room temperature to obtain the composite preparation.

[0041] Example 2

[0042] A composite preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: 1.6 parts of hydroxypropyl methylcellulose, 0.45 parts of montmorillonite, 0.52 parts of chitosan, 4.5 parts of astragalus polysaccharide powder, 4 parts of xylooligosaccharide powder, 3.5 parts of compound enzyme agent, 3.2 parts of compound bacterium agent, 2.3 parts of egg yolk antibody, 2.5 parts of yeast selenium powder; the rest are exactly the same.

[0043] Example 3

[0044] A composite preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: 2.1 parts of hydroxypropyl methylcellulose, 0.65 parts of montmorillonite, 0.8 parts of chitosan, 5.8 parts of astragalus polysaccharide powder, 5.3 parts of xylooligosaccharide powder, 4.8 parts of compound enzyme agent, 4.4 parts of compound bacterium agent, 3.2 parts of egg yolk antibody, 3.6 parts of yeast selenium powder; the rest are exactly the same.

[0045] Example 4

[0046] A composite preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: 1 part of hydroxypropyl methylcellulose, 0.6 parts of montmorillonite, 0.8 parts of chitosan, 5.6 parts of astragalus polysaccharide powder, 3 parts of xylooligosaccharide powder, 2.8 parts of compound enzyme agent, 2.3 parts of compound bacterium agent, 1.5 parts of egg yolk antibody, 1.8 parts of yeast selenium powder; the rest are exactly the same.

[0047] Example 5

[0048] A composite preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: 2 parts of hydroxypropyl methylcellulose, 0.32 parts of montmorillonite, 0.35 parts of chitosan, 3.2 parts of astragalus polysaccharide powder, 5.2 parts of xylooligosaccharide powder, 2.8 parts of compound enzyme agent, 2.3 parts of compound bacterium agent, 1.5 parts of egg yolk antibody, 1.8 parts of yeast selenium powder; the rest are exactly the same.

[0049] Example 6

[0050] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method thereof, which is different from Example 1 in that: 1 part of hydroxypropyl methylcellulose, 0.65 part of montmorillonite, 0.6 part of chitosan, 3.2 parts of astragalus polysaccharide powder, 3 parts of xylooligosaccharide powder, 4.7 parts of compound enzyme agent, 4.2 parts of compound bacterium agent, 3.2 parts of egg yolk antibody, 3.5 parts of yeast selenium powder; the rest are exactly the same.

[0051] Example 7

[0052] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method thereof, which is different from Example 2 in that: the concentration of glycerol in the glycerol aqueous solution is 25 wt%; the concentration of hydroxypropyl methylcellulose in the hydroxypropyl methylcellulose solution is 3.8 wt%; the concentration of chitosan in the chitosan solution is 1.7 wt%; in the compound enzyme agent, the mass ratio of phytase with an activity unit number ≥ 50,000 IU / g, mannanase with an activity unit number ≥ 50,000 IU / g, and cellulase with an activity unit number ≥ 50,000 IU / g is 1:0.8:0.3; in the compound bacterium agent, the viable bacteria count of Bacillus subtilis ≥ 2.5×10 11 CFU / g, the viable bacteria count of Lactobacillus plantarum ≥ 2×10 11 CFU / g, and the ratio of Lactobacillus brueckii ≥ 2.5×10 11 CFU / g is 1.3:1.2:1; in step 5, keep the temperature at 25°C and stir for 2 h, and the freeze-drying conditions: first pre-cool at 0°C for 1.5 h, then cool down to -42°C and treat for 10 h, then warm up to -18°C and treat for 3 h, and then warm up to room temperature; the rest are exactly the same.

[0053] Example 8

[0054] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method thereof, which is different from Example 2 in that: the concentration of glycerol in the glycerol aqueous solution is 35 wt%; the concentration of hydroxypropyl methylcellulose in the hydroxypropyl methylcellulose solution is 4.3 wt%; the concentration of chitosan in the chitosan solution is 1.4 wt%; in the compound enzyme agent, the mass ratio of phytase with an activity unit number ≥ 50,000 IU / g, mannanase with an activity unit number ≥ 50,000 IU / g, and cellulase with an activity unit number ≥ 50,000 IU / g is 1:1:0.6; in the compound bacterium agent, the viable bacteria count of Bacillus subtilis ≥ 2.5×10 11 CFU / g, the viable bacteria count of Lactobacillus plantarum ≥ 2×10 11 CFU / g, and the ratio of Lactobacillus brueckii ≥ 2.5×10 11 CFU / g is 2.5:2:1; in step 5, keep the temperature at 35°C and stir for 1 h, and the freeze-drying conditions: first pre-cool at -5°C for 1 h, then cool down to -50°C and treat for 8 h, then warm up to -25°C and treat for 2 h, and then warm up to room temperature; the rest are exactly the same.

[0055] Comparative Example 1

[0056] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: the amount of astragalus polysaccharide powder increases to 6.3 parts; the rest are exactly the same.

[0057] Comparative Example 2

[0058] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: the amount of xylooligosaccharide powder increases to 6 parts; the rest are exactly the same.

[0059] Comparative Example 3

[0060] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: the amount of compound enzyme preparation increases to 5.3 parts; the rest are exactly the same.

[0061] Comparative Example 4

[0062] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: the amount of compound bacterium preparation increases to 5 parts; the rest are exactly the same.

[0063] Comparative Example 5

[0064] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 3 in that: the amount of yeast selenium powder increases to 4.2 parts; the rest are exactly the same.

[0065] Comparative Example 6

[0066] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: the amount of egg yolk antibody increases to 3.8 parts; the rest are exactly the same.

[0067] Comparative Example 7

[0068] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 1 in that: the amount of montmorillonite increases to 0.85 parts; the rest are exactly the same.

[0069] Comparative Example 8

[0070] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 3 in that: the amount of astragalus polysaccharide powder decreases to 2.8 parts; the rest are exactly the same.

[0071] Comparative Example 9

[0072] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 3 in that: the amount of xylooligosaccharide powder decreases to 2.3 parts; the rest are exactly the same.

[0073] Comparative Example 10

[0074] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, different from Example 3 in that: the compound enzyme agent is reduced to 2 parts; the rest are exactly the same.

[0075] Comparative Example 11

[0076] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, different from Example 3 in that: the compound bacterium agent is reduced to 1.5 parts; the rest are exactly the same.

[0077] Comparative Example 12

[0078] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, different from Example 3 in that: the yeast selenium powder is reduced to 1 part; the rest are exactly the same.

[0079] Comparative Example 13

[0080] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, different from Example 3 in that: the egg yolk antibody is reduced to 0.8 part; the rest are exactly the same.

[0081] Comparative Example 14

[0082] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, different from Example 3 in that: the montmorillonite is reduced to 0.15 part; the rest are exactly the same.

[0083] Comparative Example 15

[0084] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, different from Example 2 in that: the compound enzyme agent is composed of mannanase with an activity unit number of ≥50,000 IU / g and cellulase with an activity unit number of ≥50,000 IU / g in a mass ratio of 0.85:0.55; the rest are exactly the same.

[0085] Comparative Example 16

[0086] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, different from Example 2 in that: the compound enzyme agent is composed of plant enzyme with an activity unit number of ≥50,000 IU / g and cellulase with an activity unit number of ≥50,000 IU / g in a mass ratio of 1:0.55; the rest are exactly the same.

[0087] Comparative Example 17

[0088] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, different from Example 2 in that: the compound bacterium agent is composed of Lactobacillus plantarum with a viable bacteria count of ≥2×10 11 CFU / g and Lactobacillus plantarum with a viable bacteria count of ≥2.5×10 11Lactobacillus delbrueckii subsp. lactis at CFU / g is composed in a mass ratio of 1.5:1; the rest is exactly the same.

[0089] Comparative Example 18

[0090] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 2 in that: the compound bacterium agent is composed of Bacillus subtilis with viable count ≥ 2.5×10 11 CFU / g and Lactobacillus plantarum with viable count ≥ 2×10 11 CFU / g in a mass ratio of 1.85:1; the rest is exactly the same.

[0091] Comparative Example 19

[0092] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 2 in that: the compound bacterium agent is composed of Bacillus subtilis with viable count ≥ 2.5×10 11 CFU / g and Lactobacillus plantarum with viable count ≥ 2×10 11 CFU / g in a mass ratio of 1.8:1.5; the rest is exactly the same.

[0093] Comparative Example 20

[0094] A compound preparation for preventing and treating bacterial diseases of livestock and poultry and its preparation method, which is different from Example 2 in that:

[0095] Step 2: Dissolve 1 part of hydroxypropyl methylcellulose in 28wt% glycerol aqueous solution to form a 4wt% hydroxypropyl methylcellulose solution, and dissolve 0.35 part of chitosan in 28wt% glycerol aqueous solution to form a 1.55wt% chitosan solution;

[0096] Step 3: Add 3.2 parts of astragalus polysaccharide powder, 2.9 parts of xylooligosaccharide powder, 2.5 parts of compound enzyme agent, 2 parts of the above compound bacterium agent, 1.3 parts of egg yolk antibody, 1.5 parts of yeast selenium powder and 0.3 part of montmorillonite, and stir evenly to obtain a uniform mixed solid material;

[0097] Step 4: After mixing with the chitosan solution, add the mixed solid material, stir and mix, and then perform high-pressure homogenization to form a uniformly dispersed mixed suspension;

[0098] Step 5: While stirring the above hydroxypropyl methylcellulose solution, add the mixed suspension and mix. The material liquid is stirred at 30°C for 1.5 h, and then freeze-dried: first pre-cool at -4°C for 75 min, then cool down to -45°C for 9 h, then warm up to -22°C for 2.5 h, and then warm up to room temperature to obtain the compound preparation; the rest is exactly the same.

[0099] Effect test: In vitro test for inhibitory effect on harmful bacteria

[0100] Control group: Four common harmful bacteria (Riemerella anatipestifer, Escherichia coli, Salmonella, Staphylococcus aureus) were selected. Riemerella anatipestifer, Escherichia coli, Salmonella, and Staphylococcus aureus were respectively activated and cultured for 24 h. After the bacterial solution was eluted, it was added to the blank medium, and the OD600nm value was adjusted to about 0.3. Without adding any preparations, then the growth effect (OD600nm value) of the harmful bacteria at 24 hours was measured using an enzyme-labeled instrument, as Figure 1 shown.

[0101] Effect Example 1: Riemerella anatipestifer, Escherichia coli, Salmonella, and Staphylococcus aureus were respectively activated and cultured for 24 h. After the bacterial solution was eluted, it was added to the blank medium, and the OD600nm value was adjusted to about 0.3. Then the compound preparations prepared in Examples 1 to 8 were respectively added, and the addition concentration of the compound preparation was 0.3 mg / mL. The growth effect (OD600nm value) of the harmful bacteria at 24 hours was measured using an enzyme-labeled instrument as Figure 2 shown.

[0102] It was found from Figure 2 and Figure 1 that the compound preparations for preventing and treating bacterial diseases of livestock and poultry prepared in Examples 1 to 8 of the present invention are synergistically compounded with astragalus polysaccharide powder, xylooligosaccharide powder, compound enzyme preparation, compound bacterial preparation, yeast selenium powder, and egg yolk antibody, and can effectively inhibit the growth and reproduction of viruses such as Riemerella, Escherichia coli, and Salmonella, which is beneficial to promoting and regulating the immune function of the animal itself and improving the body resistance of the animal.

[0103] Effect Example 2: Riemerella anatipestifer, Escherichia coli, Salmonella, and Staphylococcus aureus were respectively activated and cultured for 24 h. After the bacterial solution was eluted, it was added to the blank medium, and the OD600nm value was adjusted to about 0.3. Then the compound preparations prepared in Example 1 and Comparative Examples 1 to 7 were respectively added, and the addition concentration of the compound preparation was 0.3 mg / mL. The growth effect (OD600nm value) of the harmful bacteria at 24 hours was measured using an enzyme-labeled instrument as Figure 3 shown.

[0104] It was found from Figure 3It was found that, compared with Example 1, in Comparative Example 1, too much Astragalus polysaccharide powder was used, resulting in a reduced inhibitory effect on harmful bacteria. This may be because too much Astragalus polysaccharide powder affects its synergistic effect with xylo-oligosaccharide, compound bacterial agent, etc., thus allowing harmful bacteria to grow. In Comparative Example 2, too much xylo-oligosaccharide powder was used, which would promote the over-proliferation of harmful bacteria. This may be because too much xylo-oligosaccharide powder reduces the probiotic effects of Astragalus polysaccharide powder, compound bacterial agent, egg yolk antibody, etc., thus allowing harmful bacteria to grow. In Comparative Example 3, too much compound enzyme preparation was used, which also reduced the inhibitory effect on harmful bacteria. This may be because too much compound enzyme preparation destroys the active ingredients of Astragalus polysaccharide powder, egg yolk antibody, xylo-oligosaccharide powder, etc., affecting the probiotic effect of the compound bacterial agent, thus allowing harmful bacteria to grow. In Comparative Example 4, too much compound bacterial agent was used, which also reduced the inhibitory effect on harmful bacteria. This may be because the synergistic effect between too much compound bacterial agent and Astragalus polysaccharide is too strong, affecting the synergistic effect between the compound bacterial agent and the compound bacterial agent, egg yolk antibody, etc., thus allowing harmful bacteria to grow. In Comparative Example 5, too much yeast selenium powder was used, which also led to a reduced inhibitory effect on harmful bacteria. This may be because too much yeast selenium powder reduces the utilization rate of Astragalus polysaccharide powder, xylo-oligosaccharide powder, compound bacterial agent, compound enzyme preparation, etc., interfering with the regulatory effect of egg yolk antibody, thus allowing harmful bacteria to grow. In Comparative Example 6, too much egg yolk antibody was used, which also led to a reduced inhibitory effect on harmful bacteria. This may be because too much egg yolk antibody affects the immune effects of Astragalus polysaccharide powder, egg yolk antibody, egg yolk antibody, etc., reducing the protective effect on beneficial bacteria, thus allowing harmful bacteria to grow. In Comparative Example 7, too much montmorillonite was used, resulting in a reduced inhibitory effect on harmful bacteria. This may be because too much montmorillonite adsorbs active ingredients such as egg yolk antibody and enzyme preparation, reducing the effect of the compound preparation, thus allowing harmful bacteria to grow.

[0105] Effect Example 3: Riemerella anatipestifer, Escherichia coli, Salmonella, and Staphylococcus aureus were respectively taken and activated and cultured for 24 h. After the bacterial liquid was eluted, it was added to a blank culture medium, and the OD600nm value was adjusted to about 0.3. Then, the compound preparations of Example 3 and Comparative Examples 8 to 14 were respectively added. The addition concentration of the compound preparation was 0.3 mg / mL, and a microplate reader was used to measure the 24-hour growth effect (OD600nm value) of harmful bacteria as Figure 4 shown.

[0106] From Figure 4It was found that, compared with Example 3, too little astragalus polysaccharide powder was used in Comparative Example 8, which affected the inhibitory effect on harmful bacteria. This may be because the weak synergistic effect of too little astragalus polysaccharide powder with the compound bacteria agent and egg yolk antibody led to insufficient antibacterial effect, thus weakening the inhibition of harmful bacteria; too little xylo-oligosaccharide powder was used in Comparative Example 2, which also affected the inhibitory effect on harmful bacteria. This may be because the insufficient synergistic probiotic effect of too little xylo-oligosaccharide powder with astragalus polysaccharide powder, compound bacteria agent, etc. led to weakening of the inhibition of harmful bacteria; too little compound enzyme agent was used in Comparative Example 3, and it had little effect on the inhibitory effect on harmful bacteria. Enzymes can effectively decompose anti-nutritional factors, and reducing feed utilization is beneficial to the absorption of other components. Therefore, an appropriate amount of compound enzyme agent is beneficial to improving the utilization rate of the compound preparation in livestock and poultry feeding; too little compound bacteria agent was used in Comparative Example 4 and too little yeast selenium powder was used in Comparative Example 5, which also affected the inhibitory effect on harmful bacteria. This may be because too little compound bacteria agent and yeast selenium powder reduced the synergistic antibacterial effect of components such as xylo-oligosaccharide and astragalus polysaccharide, thus weakening the inhibition of harmful bacteria; too little egg yolk antibody was used in Comparative Example 6, which also affected the inhibitory effect on harmful bacteria. This may be because too little egg yolk antibody could not promote the probiotic effects of astragalus polysaccharide powder, egg yolk antibody, etc., thus weakening the inhibition of harmful bacteria; too little montmorillonite was used in Comparative Example 7, which also affected the inhibitory effect on harmful bacteria. This may be because too little montmorillonite reduced the adsorption of harmful bacteria and reduced the protection of beneficial active ingredients, thus weakening the inhibition of harmful bacteria.

[0107] Effect Example 4: Riemerella anatipestifer, Escherichia coli, Salmonella, and Staphylococcus aureus were separately taken and activated and cultured for 24 h. After the bacterial solution was eluted, it was added to a blank culture medium, and the OD600nm value was adjusted to about 0.3. Then, the compound preparations of Example 2 and Comparative Examples 15 to 20 were added respectively, and the addition concentration of the compound preparation was 0.3 mg / mL. The growth effect (OD600nm value) of harmful bacteria after 24 hours was measured using a microplate reader as Figure 5 shown.

[0108] It was found from Figure 5 that, compared with Example 2, the lack of phytase or mannanase in the compound enzyme agent of the compound preparations in Comparative Examples 15 to 16 would reduce the synergistic effect among the components of the compound preparation and reduce the inhibitory effect on harmful bacteria; the lack of Bacillus subtilis, Lactobacillus plantarum, or Lactobacillus brueckii in the compound bacteria agent of the compound preparations in Comparative Examples 17 to 19 would affect the synergistic effect among the components of the compound preparation and also reduce the inhibitory effect on harmful bacteria; during the preparation of Comparative Example 20, the active ingredients such as the compound enzyme agent, egg yolk antibody, and compound enzyme agent were treated by high-pressure homogenization, and the active ingredients might be damaged, weakening the active effect of the compound preparation, thus reducing the inhibitory effect on harmful bacteria.

[0109] In summary, the composite preparation for preventing and treating bacterial diseases of livestock and poultry according to the present invention is a composite preparation synergistically compounded with astragalus polysaccharide powder, xylooligosaccharide powder, composite enzyme preparation, composite bacterial agent, yeast selenium powder, egg yolk antibody and montmorillonite, which can effectively inhibit the growth and reproduction of viruses such as Riemerella, Escherichia coli, Salmonella, etc., is beneficial to promoting and regulating the immune function of the animal itself, improving the body resistance of the animal, and the composite preparation for preventing and treating bacterial diseases of livestock and poultry will revive and proliferate in the animal intestine, exert probiotic characteristics, which is more beneficial to improving the animal intestinal flora, maintaining intestinal health, also exerting immune regulation, enhancing the body immunity, and then promoting the body growth and development, and will improve the growth and development, feed conversion rate and meat quality of livestock and poultry; in addition, in the manufacture of such biological drugs as the composite preparation for preventing and treating bacterial diseases of livestock and poultry, it is not damaged by strong shear force, ensuring the active ingredients of the product and improving the stability of the product during the processing process.

[0110] The composite preparation for preventing and treating bacterial diseases of livestock and poultry according to the present invention can be used alone or as a feed additive for animal feed or drinking water, and has the effects of improving the immunity of animals and preventing bacterial diseases, wherein the animals include but are not limited to ducks, chickens, geese, pigs, etc.

[0111] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit and basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, it is intended to embrace all changes falling within the meaning and scope of the equivalent elements of the claims in the present invention.

[0112] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A compound preparation for preventing and treating bacterial diseases of livestock and poultry, characterized in that, It comprises the following components and their parts by weight: 3.2 - 5.8 parts of astragalus polysaccharide powder, 2.9 - 5.3 parts of xylooligosaccharide powder, 2.5 - 4.8 parts of compound enzyme preparation, 2 - 4.4 parts of compound bacterium preparation, 1.5 - 3.6 parts of yeast selenium powder, 1.3 - 3.2 parts of egg yolk antibody, 1 - 2.1 parts of hydroxypropyl methylcellulose, 0.35 - 0.8 part of chitosan and 0.3 - 0.65 part of montmorillonite.

2. The compound preparation for preventing and treating bacterial diseases of livestock and poultry according to claim 1, characterized in that, The compound enzyme preparation is composed of plant enzyme, mannanase and cellulase.

3. A compound preparation for preventing and treating bacterial diseases of livestock and poultry according to claim 1 or 2, characterized in that, The compound enzyme preparation is composed of plant enzyme, mannanase and cellulase according to the mass ratio of 1:0.8 - 1:0.3 - 0.

6.

4. A compound preparation for preventing and treating bacterial diseases of livestock and poultry, characterized in that, The compound bacterium preparation is composed of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus brittanicus.

5. The composite preparation for preventing and treating bacterial diseases of livestock and poultry according to claim 1 or 4, characterized in that, The compound bacterium preparation is composed of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus brittanicus according to the mass ratio of 1.3 - 2.5:1.2 - 2:

1.

6. A preparation method of the composite preparation for preventing and treating bacterial diseases of livestock and poultry according to claim 1, characterized in that, (1) Dissolve hydroxypropyl methylcellulose in glycerol aqueous solution to form a hydroxypropyl methylcellulose solution, then add montmorillonite and perform high-pressure homogenization to obtain a uniformly dispersed mixed suspension; (2) Dissolve chitosan in glycerol aqueous solution to form a chitosan solution, then add astragalus polysaccharide powder and xylooligosaccharide powder and stir evenly to obtain a uniform mixed solution; (3) Add the compound enzyme preparation, compound bacterium preparation, egg yolk antibody and yeast selenium powder to the above mixed solution, stir and disperse evenly to obtain a uniformly mixed emulsion; (4) Add the mixed suspension while stirring the mixed emulsion, then keep stirring at 25 - 35 °C for 1 - 2 h, and then perform freeze-drying to obtain the compound preparation.

7. The preparation method of a composite preparation for preventing and treating bacterial diseases of livestock and poultry according to claim 6, characterized in that, The concentration of hydroxypropyl methylcellulose in the hydroxypropyl methylcellulose solution is 3.8 - 4.3 wt%.

8. The preparation method of a compound preparation for preventing and treating bacterial diseases of livestock and poultry according to claim 6, characterized in that, The concentration of chitosan in the chitosan solution is 1.4 - 1.7 wt%.

9. The preparation method of a compound preparation for preventing and treating bacterial diseases of livestock and poultry according to claim 6, characterized in that, The concentration of glycerol in the glycerol aqueous solution is 25 - 35 wt%.

10. The preparation method of a compound preparation for preventing and treating bacterial diseases of livestock and poultry according to claim 6, characterized in that, Freeze-drying conditions: First, pre-cool at -5 - 0 °C for 1 - 1.5 h, then cool down to -50 - -42 °C and process for 8 - 10 h, then heat up to -25 - -18 °C and process for 2 - 3 h, and then heat up to room temperature.

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