Detection equipment of nucleic acid extraction and amplification all-in-one machine

Abstract

The invention discloses detection equipment of a nucleic acid extraction and amplification all-in-one machine. The detection equipment comprises a bracket, an amplification device, an extraction device, a fluorescence detection device and a driving device, the amplification device is arranged on the bracket and is used for adjusting the temperature; the extraction device is mounted on the amplification device and comprises a test tube mounting assembly, a first driving part and a magnetic attraction part, the test tube mounting assembly is provided with a first mounting hole, the magnetic attraction part is arranged on the hole wall of the first mounting hole, and the first driving part is used for driving the test tube mounting assembly to rotate; the magnetic attraction piece is driven to rotate around the peripheral side of a test tube in the test tube mounting assembly and is used for extracting nucleic acid; the fluorescence detection device is mounted on the bracket and is used for performing fluorescence detection on the test tube; the output end of the driving device is connected with the amplification device and is used for driving the amplification device and the extraction device to rotate relative to the fluorescence detection device. According to the invention, the extraction, amplification and fluorescence detection functions of nucleic acid are integrated, so that the efficiency is improved, and the detection time is shortened.

Description

Technical Field

[0001] The present invention relates to the field of nucleic acid technology, and particularly to a nucleic acid extraction, amplification and detection integrated device. Background Art

[0002] At present, the processes of nucleic acid extraction, amplification and detection are all completed manually in the laboratory. To perform a nucleic acid detection, multiple steps such as extraction, amplification, and PCR fluorescence detection are required. Moreover, to prevent aerosol contamination and ensure biosafety, the existing nucleic acid extraction process and the operation of amplification PCR detection are completed in laboratories in different partitions. Multiple devices such as a nucleic acid extractor and a PCR amplifier are needed. Each device is a single product. Manual operations are carried out in multiple independent partitions and different devices, increasing the complexity and tediousness of the nucleic acid extraction and detection process. This makes it inevitable for manual operations to have operational errors. At the same time, the number of nucleic acid extractions and detections that can be performed manually at one time is small and the required time is long, further reducing the efficiency of manual operations. Completing different steps in laboratories in different partitions also cannot well avoid aerosol contamination and has low safety. Summary of the Invention

[0003] The technical problem to be solved by the present invention is that the extraction, amplification and detection of nucleic acids are completed in laboratories in different partitions, the process is cumbersome, the operation efficiency is low, and aerosol contamination cannot be well avoided.

[0004] To solve the above technical problem, the present invention provides a nucleic acid extraction, amplification and detection integrated device, including:

[0005] A bracket;

[0006] An amplification device, arranged on the bracket and used for temperature regulation;

[0007] An extraction device, installed on the amplification device. The extraction device includes a test tube installation component, a first driving member and a magnetic attraction member. The test tube installation component has a first installation hole, the magnetic attraction member is arranged on the hole wall of the first installation hole, and the first driving member is installed on the bracket and used for driving the test tube installation component to rotate, so as to drive the magnetic attraction member to rotate around the outer periphery of the test tube located in the test tube installation component and perform nucleic acid extraction;

[0008] A fluorescence detection device, installed on the bracket and used for fluorescence detection of the test tube; and

[0009] A driving device, installed on the bracket, and the output end of the driving device is connected to the amplification device and used for driving the amplification device and the extraction device to rotate relative to the fluorescence detection device.

[0010] In some embodiments, the extraction device further includes a gear disk, the test tube mounting assembly includes a gear member, and an output shaft of the first driving member passes through the amplification device and is connected to the gear disk;

[0011] The gear member has the first mounting hole, the gear disk meshes with the gear member, and the magnetic attracting member is arranged on the gear member.

[0012] In some embodiments, the first mounting hole penetrates through two ends of the gear member, a second mounting hole is formed in a hole wall of the first mounting hole, and the magnetic attracting member is arranged in the second mounting hole.

[0013] In some embodiments, the extraction device further includes a chassis and a top plate, a receiving cavity is formed between the chassis and the top plate, the gear disk is arranged in the receiving cavity, the chassis is arranged on the amplification device, and a third mounting hole for the bottom of the test tube to pass through is formed in the chassis.

[0014] In some embodiments, fourth mounting holes are formed in a side surface of the gear member facing the chassis and a side surface of the gear member facing the top plate, and balls are arranged in the fourth mounting holes;

[0015] The test tube mounting assembly further includes a fitting, the fitting is arranged on the chassis, a first sliding groove is formed in a side surface of the fitting facing the top plate, and the third mounting hole penetrates through the first sliding groove; a second sliding groove corresponding to the gear member is formed in a side surface of the top plate facing the chassis;

[0016] The gear member is arranged between the top plate and the fitting, the gear member and the fitting are correspondingly arranged, and the gear member can rotate on the first sliding groove and the second sliding groove through the balls.

[0017] In some embodiments, the test tube mounting assembly further includes a first heating member, the first heating member is arranged on the top plate and corresponds to the gear member.

[0018] In some embodiments, the test tube mounting assembly further includes a heat insulation member, and the heat insulation member is sleeved on an outer peripheral side of the first heating member.

[0019] In some embodiments, the number of the test tube mounting assemblies is multiple, and the multiple test tube mounting assemblies are arranged around a circumferential side of the gear disk to form a test tube mounting group;

[0020] Wherein, the number of the test tube mounting groups is n, and n groups of the test tube mounting groups are arranged in sequence along a radial direction of the gear disk, where n≥1.

[0021] In some embodiments, the amplification device includes a heating plate, a second heating element and a heat conductive element, the second heating element is disposed on the heating plate and located below the extraction device, and the heat conductive element and the first driving element are disposed on a side of the heating plate away from the second heating element.

[0022] In some embodiments, there are multiple second heating elements, and the multiple second heating elements are spliced around the axis of the heating plate to form an annular structure.

[0023] In some embodiments, a cooling fan is further included, wherein the cooling fan is installed on the bracket, and an air intake port of the cooling fan faces the amplification device.

[0024] In some embodiments, a light guide device is arranged between the extraction device and the amplification device, and the light guide device has a fifth mounting hole and a first light guide hole. The fifth mounting hole is arranged corresponding to the third mounting hole. The bottom of the test tube extends into the light guide device through the fifth mounting hole, and the light emitted by the fluorescence detection device is irradiated onto the bottom of the test tube through the first light guide hole.

[0025] In some embodiments, the light guide device includes a light guide disc and a light guide column. The side of the light guide disc facing the fluorescence detection device is provided with the first light guide hole for the light emitted by the fluorescence detection device to pass through. The side of the light guide disc facing the extraction device is provided with the fifth mounting hole. The light guide column is arranged in the light guide disc and is located between the first light guide hole and the fifth mounting hole, and is used to irradiate the light emitted by the fluorescence detection device to the bottom of the test tube through the first light guide hole.

[0026] In some embodiments, a center line of the first light guide hole is arranged perpendicular to a center line of the fifth mounting hole.

[0027] In some embodiments, the first light guide holes have a plurality of holes, and the plurality of first light guide holes are arranged at intervals around the axis of the optical disk;

[0028] There are a plurality of the fifth mounting holes, the plurality of the fifth mounting holes are arranged at intervals around the axis of the optical guide plate, and the first light guide holes are arranged in one-to-one correspondence with the fifth mounting holes;

[0029] A light guide column is disposed between each of the first light guide holes and the corresponding fifth mounting hole.

[0030] In some embodiments, the optical disc further includes a light guide tube, one end of the light guide tube is connected to the first light guide hole, the other end of the light guide tube is connected to the fifth mounting hole, and the light guide column is disposed in the light guide tube.

[0031] In some embodiments, the fluorescence detection device includes a transmitting end;

[0032] The diameter of the light guide disc is smaller than the diameter of the extraction device, and the diameter of the light guide disc is smaller than the diameter of the amplification device. A groove structure is defined between the extraction device, the light guide disc and the amplification device, and the transmitting end is located in the groove structure.

[0033] In some embodiments, the fluorescence detection device includes a light emitting assembly, an optical fiber and an optical fiber transmitter. The light emitting assembly and the optical fiber transmitter are mounted on the bracket. The light emitting assembly and the optical fiber transmitter are connected by the optical fiber. The optical fiber transmitter has the transmitting end, and the light emitted from the transmitting end irradiates the bottom of the test tube through the first light guide hole and the light guide column.

[0034] In some embodiments, there are multiple light emitting assemblies, and the multiple light emitting assemblies are arranged at intervals around the output shaft of the driving device;

[0035] There are multiple optical fiber transmitters, and the multiple optical fiber transmitters are arranged at intervals around the axis of the bracket.

[0036] In some embodiments, the fluorescence detection device further includes a driving component. The light emitting assembly includes multiple light sources, multiple kinds of filter plates and a first transmission member. The first transmission member has a transmission end connected to the driving component and an installation end located on the outgoing light path. The multiple light sources are installed at intervals around the axis of the installation end, and a filter plate is correspondingly arranged at the front end of the emission head of each light source;

[0037] Whenever the driving component drives the first transmission member to rotate once, the first transmission member drives each light source to rotate to the outgoing light path to emit light with a corresponding wavelength.

[0038] In some embodiments, the driving component includes a second driving member and a conveyor belt, and the first transmission member and the second driving member are synchronously driven by the conveyor belt.

[0039] In some embodiments, the driving device includes a third driving member, a synchronous belt and a second transmission member. The third driving member is mounted on the bracket, and the second transmission member is mounted at the bottom of the amplification device. The third driving member and the second transmission member are synchronously driven by the synchronous belt to drive the amplification device to rotate.

[0040] Compared with the prior art, the nucleic acid extraction and amplification integrated detection device according to the embodiments of the present invention has the following beneficial effects:

[0041] The extraction device in the embodiment of the present invention is used for nucleic acid extraction of a test tube placed in a placement hole. Among them, a first driving member is used to drive a magnetic attraction member to rotate around the outer periphery of the test tube placed in a first installation hole, and provide a magnetic attraction force through the magnetic attraction member arranged outside the first installation hole to adsorb magnetic beads in the test tube, and drive the magnetic beads to rotate to complete nucleic acid extraction; an amplification device is used to adjust the temperature at the bottom of the test tube to achieve nucleic acid amplification; the driving device drives the test tube located on the extraction device to rotate relative to the fluorescence detection device, so that the fluorescence detection device can perform fluorescence detection on the test tube.

[0042] By integrating the functions of nucleic acid extraction, amplification, and fluorescence detection in one body, the present invention eliminates the need for nucleic acid extraction, amplification, and fluorescence detection operations in laboratories with different partitions, improves efficiency, shortens the detection time, and avoids the problem of aerosol contamination caused by the need to move to different partitions for work. Description of the Drawings

[0043] Figure 1 It is a schematic structural diagram of a nucleic acid extraction and amplification integrated detection device provided by an embodiment of the present invention;

[0044] Figure 2 It is a partial exploded view of the nucleic acid extraction and amplification integrated detection device provided by an embodiment of the present invention from a first angle;

[0045] Figure 3 It is an exploded view of the magnetic attraction member and the gear member provided by an embodiment of the present invention;

[0046] Figure 4 It is an exploded view of the nucleic acid extraction and amplification integrated detection device provided by an embodiment of the present invention;

[0047] Figure 5 It is an exploded view of the amplification device, the extraction device, and the light guide device from a first angle provided by an embodiment of the present invention;

[0048] Figure 6 It is an exploded view of the amplification device, the extraction device, and the light guide detection device from a second angle provided by an embodiment of the present invention;

[0049] Figure 7 It is an exploded view of the extraction device provided by an embodiment of the present invention;

[0050] Figure 8 It is an exploded view of the top plate, the gear plate, and the gear member provided by an embodiment of the present invention;

[0051] Figure 9 It is an exploded view of the top plate, the first heating member, and the heat insulation member provided by an embodiment of the present invention;

[0052] Figure 10is a local enlarged view of part A circled in the embodiment of the present invention; Figure 7

[0053] Figure 11 is a local enlarged view of part B circled in the embodiment of the present invention; Figure 7

[0054] Figure 12 is a local enlarged view of part C circled in the embodiment of the present invention; Figure 9

[0055] Figure 13 is an exploded view of the driving device and the amplification device provided by the embodiment of the present invention;

[0056] Figure 14 is a schematic structural diagram of the amplification device, extraction device and light guide device provided by the embodiment of the present invention;

[0057] Figure 15 is a partial exploded view of the bracket, fluorescence detection device and heat dissipation fan provided by the embodiment of the present invention;

[0058] Figure 16 is a partial exploded view of the nucleic acid extraction and amplification integrated detection device from a second angle provided by the embodiment of the present invention;

[0059] Figure 17 is an exploded schematic diagram of the light guide plate provided by the embodiment of the present invention;

[0060] Figure 18 is a local enlarged view of part D circled in the embodiment of the present invention; Figure 2

[0061] In the figure, 1 is the bracket; 11 is the upper bracket; 12 is the lower bracket;

[0062] 2 is the amplification device; 21 is the heating plate; 2101 is the third through hole; 22 is the second heating element; 23 is the heat conducting member;

[0063] 3 is the extraction device; 31 is the test tube installation assembly; 3101 is the first installation hole; 3102 is the gear member; 31021 is the second installation hole; 31022 is the fourth installation hole; 31023 is the main body; 31024 is the ratchet tooth; 3103 is the mating member; 31031 is the first sliding groove; 3104 is the first heating element; 3105 is the heat insulation member; 31051 is the first base; 31052 is the first installation groove; 31053 is the first connecting member; 310531 is the second base; 310532 is the second installation groove; 32 is the first driving member; 33 is the magnetic attracting member; 34 is the gear disk; 3401 is the first through hole; 35 is the chassis; 3501 is the third installation hole; 3502 is the second through hole; 36 is the top plate; 3601 is the second sliding groove; 37 is the second connecting member;​​​​

[0064] 4. Fluorescence detection device; 41. Light emission component; 4101. First transmission member; 42. Optical fiber; 43. Optical fiber transmitter; 44. Driving component; 4401. Second driving member; 4402. Conveyor belt;

[0065] 5. Driving device; 51. Third driving member; 52. Timing belt; 53. Second transmission member;

[0066] 6. Cooling fan;

[0067] 7. Light guiding device; 71. Fifth mounting hole; 72. First light guiding hole; 73. Light guiding disc; 7301. Light guiding tube; 73011. Light guiding part; 7302. First disc; 7303. Second disc; 74. Light guiding column. Detailed implementation manners

[0068] The following combines the accompanying drawings and embodiments to further describe in detail the specific implementation manners of the present invention. The following embodiments are used to illustrate the present invention, but are not used to limit the scope of the present invention.

[0069] As Figure 1 shown, the embodiment of the present invention provides a nucleic acid extraction, amplification and integrated detection device for nucleic acid extraction, amplification and fluorescence detection. In the actual application process, a test tube is placed on the nucleic acid extraction, amplification and integrated detection device, and the nucleic acid extraction, amplification and integrated detection device performs nucleic acid extraction, amplification and fluorescence detection on the nucleic acid solution in the test tube.

[0070] Please refer to Figures 1 to 3 、 Figure 5 , the nucleic acid extraction, amplification and integrated detection device includes a bracket 1, an amplification device 2, an extraction device 3, a fluorescence detection device 4 and a driving device 5. The bracket 1 in this embodiment serves as an installation structure and plays a role in carrying the amplification device 2, the extraction device 3, the fluorescence detection device 4 and the driving device 5; the extraction device 3 is used for extracting nucleic acid from the nucleic acid solution in the test tube. In this embodiment, magnetic beads are placed in the test tube, and the magnetic beads are used to fully bind to the lysed nucleic acid. The extraction device 3 includes a test tube installation component 31, a first driving member 32 and a magnetic attracting member 33. The test tube installation component 31 has a first mounting hole 3101 for placing the test tube (as Figure 3As shown in the figure, the magnetic attraction member 33 is disposed on the hole wall of the first mounting hole 3101. The magnetic attraction member 33 provides a magnetic attraction force to adsorb the magnetic beads in the test tube. The first driving member 32 is mounted on the bracket 1 and is used to drive the test tube mounting assembly 31 to rotate, so as to drive the magnetic attraction member 33 to rotate around the outer peripheral side of the test tube located in the test tube mounting assembly 31. The magnetic beads rotate accordingly, driving the nucleic acid to complete washing and purification in the test tube, so as to complete the extraction of the nucleic acid. The extraction device 3 is mounted on the amplification device 2, and the amplification device 2 is used to adjust the temperature of the bottom of the test tube to realize the amplification of the nucleic acid in the test tube. In this embodiment, the fluorescence detection device 4 and the driving device 5 are mounted on the bracket 1, and the output end of the driving device 5 is connected to the amplification device 2. The amplification device 2 is disposed on the bracket 1 through the driving device 5. The driving device 5 is used to drive the amplification device 2 and the extraction device 3 to rotate relative to the fluorescence detection device 4, and the fluorescence detection device 4 emits light to the test tube to perform fluorescence detection on the nucleic acid in the test tube.

[0071] In this embodiment, by integrating the functions of nucleic acid extraction, amplification, and fluorescence detection into one, there is no need to perform nucleic acid extraction, amplification, and fluorescence detection operations in laboratories in different partitions, which improves the efficiency, shortens the detection time, and avoids the problem of aerosol pollution caused by the need to carry to different partitions for work.

[0072] Please refer to Figure 4 As shown in the figure, the extraction device 3 further includes a gear disk 34. A first through hole 3401 is formed in the gear disk 34, and the output shaft of the first driving member 32 passes through the amplification device 2 and is fitted and installed with the first through hole 3401 of the gear disk 34 to realize the connection between the output shaft of the first driving member 32 and the gear disk 34. The test tube mounting assembly 31 includes a gear member 3102. The gear disk 34 meshes with the gear member 3102. The gear member 3102 has a first mounting hole 3101, and the magnetic attraction member 33 is disposed on the hole wall of the first mounting hole 3101 of the gear member 3102. When the test tube is placed on the first mounting hole 3101, when the first driving member 32 drives the gear disk 34 to rotate to drive the gear member 3102 to rotate, the magnetic beads in the test tube rotate accordingly.

[0073] Such as Figure 3As shown, the gear member 3102 includes a columnar main body 31023. The main body 31023 has a first mounting hole 3101 penetrating through both ends thereof. At one end of the main body 31023, a ratchet 31024 is provided and meshes with the gear disk 34. The ratchet 31024 is arranged on the circumferential side of the main body 31023. A second mounting hole 31021 is formed in the hole wall of the first mounting hole 3101. A magnetic attracting member 33 is arranged in the second mounting hole 31021. The magnetic beads in the test tube are adsorbed by the magnetic adsorption force of the magnetic attracting member 33. When the gear disk 34 drives the main body 31023 to rotate, the position of the magnetic attracting member 33 also moves with the rotation of the main body 31023, thereby driving the magnetic beads in the test tube to rotate accordingly, and thus completing the nucleic acid extraction process. Preferably, the magnetic attracting member 33 is a magnet.

[0074] Please refer to Figures 4 to 8 , the extraction device 3 further includes a chassis 35 and a top plate 36. A receiving cavity is formed between the chassis 35 and the top plate 36. The gear disk 34 is arranged in the receiving cavity. In this embodiment, a second through hole 3502 is provided on the chassis 35 for the output shaft of the first driving member 32 to pass through and be connected to the gear disk 34. The bottom of the gear member 3102 is cooperatively installed with the chassis 35, and the top of the gear member 3102 is cooperatively installed with the top plate 36.

[0075] In this embodiment, a third mounting hole 3501 for the bottom of the test tube to pass through is provided on the chassis 35 (as Figure 10 shown). At this time, the part of the bottom of the test tube passing through the third mounting hole 3501 is exposed outside the extraction device 3, and this part is located above the amplification device 2. The amplification device 2 performs temperature regulation such as heating and cooling on this part to realize nucleic acid amplification.

[0076] It can be understood that bolt holes are formed at corresponding positions on the top plate 36 and the chassis 35, and the two are connected by bolts; in addition, hollow mounting columns can also be provided on the chassis 35, and the bolts pass through the bolt holes of the top plate 36 and are connected to the mounting columns. The specific connection method is not limited herein.

[0077] Please refer to Figure 7 , Figure 8 and Figure 10, the test tube mounting assembly 31 further includes a fitting 3103. The fitting 3103 is disposed on the chassis 35. The gear member 3102 is disposed between the top plate 36 and the fitting 3103, and the gear member 3102 is correspondingly disposed with the fitting 3103. In addition, a fourth mounting hole 31022 is provided on one side of the gear member 3102 facing the chassis 35, and a ball is disposed in the fourth mounting hole 31022. A first chute 31031 is formed on one side of the fitting 3103 facing the top plate 36, and the gear member 3102 can rotate on the first chute 31031 through the ball. When the gear member 3102 rotates, the ball rotates along the shape of the first chute 31031. In this embodiment, by providing the fitting 3103, not only the support function for the installation of the gear member 3102 is provided, but also the smooth movement of the gear member 3102 during rotation can be prevented. In this embodiment, the third mounting hole 3501 penetrates through the first chute 31031 for the bottom of the test tube to pass through.

[0078] It can be understood that a fourth mounting hole 31022 is provided on one side of the gear member 3102 facing the top plate 36 in this embodiment. A second chute 3601 corresponding to the gear member 3102 is formed on one side of the top plate 36 facing the chassis 35. The gear member 3102 can rotate on the second chute 3601 through the ball. When the gear member 3102 rotates, the ball rotates along the shape of the second chute 3601.

[0079] It should be noted that a plurality of fourth mounting holes 31022 are provided at the bottom of the gear member 3102 in this embodiment to balance the installation height between various parts of the gear member 3102 and the fitting 3103; in addition, a plurality of fourth mounting holes 31022 are provided at the top of the gear member 3102 to balance the installation height between various parts of the gear member 3102 and the second chute 3601.

[0080] Please refer to Figure 9 and Figure 11 , the test tube mounting assembly 31 further includes a first heating member 3104. The first heating member 3104 is disposed on the top plate 36 and is correspondingly disposed with the gear member 3102. When the test tube is mounted on the test tube mounting assembly 31, the test tube sequentially passes through the first heating member 3104, the first mounting hole 3101 and the third mounting hole 3501, and the bottom of the test tube is exposed below the extraction device 3 so that the light emitted by the fluorescence detection device 4 can directly hit the bottom of the test tube. The first heating member 3104 is used to heat the top of the test tube to improve the sensitivity of nucleic acid extraction. It can be understood that a through hole is provided on the top plate 36, and the through hole is correspondingly disposed with the second chute 3601 and the first heating member 3104 for the test tube to pass through.

[0081] Please refer to Figure 9 and Figure 11, the test tube mounting assembly 31 further includes a heat insulation member 3105 sleeved on the outer peripheral side of the first heating member 3104, which plays a heat insulation role to prevent heat from spreading towards the middle and bottom of the test tube.

[0082] As Figure 5 shown, in some embodiments, there are multiple test tube mounting assemblies 31, and the multiple test tube mounting assemblies 31 are arranged around the circumferential side of the gear disk 34 to form a test tube mounting group. This embodiment is described by taking the gear member 3102 as an example:

[0083] In this embodiment, the multiple gear members 3102 are installed on the circumferential side of the gear disk 34 in an annular arrangement, and the gear members 3102 are respectively meshed with the gear disk 34. This method can save space during installation. At the same time, this method can drive multiple gear members 3102 to rotate synchronously by using only one gear disk 34, with fewer transmission times and high efficiency. In addition, the situation where the operation of the entire nucleic acid extraction and amplification integrated detection device is affected by the damage of one gear member 3102 will not occur, enhancing the stability of the nucleic acid extraction and amplification integrated detection device. On the other hand, the annular arrangement structure is simpler and more compact, making the heating of the amplification device 2 more concentrated, enabling more uniform heating, with a smaller temperature difference between each test tube, and improving the uniformity. Among them, the number of test tube mounting groups is n, and the n groups of test tube mounting groups are arranged in sequence along the radial direction of the gear disk 34, where n≥1.

[0084] When n is 1, the gear member 3102 is wound around the circumferential side of the gear disk 34 to form a circular ring structure; when n is 2, the gear member 3102 is wound around the circumferential side of the gear disk 34 to form a circular ring structure, which is the inner gear ring; multiple gear members 3102 are wound around the outside of the inner gear ring to form another circular ring structure, which is the outer gear ring. At this time, the outer gear ring is meshed with the inner gear ring, and the rotation of the gear disk 34 can drive the rotation of the inner gear ring, and the outer gear ring rotates accordingly, realizing the synchronous rotation of the inner gear ring and the outer gear ring.

[0085] It can be understood that the gear member 3102 located on the outer gear ring is meshed with two adjacent gear members 3102 located on the inner gear ring, that is, the gear member 3102 located on the outer gear ring is staggered with the gear member 3102 located on the inner gear ring to achieve synchronous transmission. n can also be 3, 4 or more, and the setting method of the gear member 3102 can be inferred by analogy. Preferably, n in this embodiment is 2, and each gear ring has 16 gear members 3102, which can simultaneously realize the extraction and amplification of nucleic acids from 32 samples, improving the efficiency of nucleic acid extraction and amplification, and realizing the setting of single-module multi-flux in terms of structure.

[0086] Similarly, multiple mating parts 3103 are arranged at intervals around the axis of the chassis 35, and the mating parts 3103 are arranged in one-to-one correspondence with the gear parts 3102; multiple second sliding grooves 3601 are arranged at intervals around the axis of the top plate 36, and the second sliding grooves 3601 are arranged in one-to-one correspondence with the gear parts 3102; multiple first heating parts 3104 are arranged at intervals around the axis of the top plate 36, and the first heating parts 3104 are arranged in one-to-one correspondence with the gear parts 3102. Among them, the heat insulation parts 3105 are arranged corresponding to the first heating parts 3104, that is, multiple heat insulation parts 3105 are arranged at intervals around the axis of the top plate 36, and the heat insulation parts 3105 are sleeved on the outer peripheral side of the corresponding first heating parts 3104 to play a heat insulation role and prevent the heat of the first heating parts 3104 from diffusing to the middle and bottom of the test tube.

[0087] As Figure 12 shown, the heat insulation part 3105 in this embodiment includes a first base 31051 connected to the top plate 36 and a first installation groove 31052 located on the first base 31051. The first base 31051 is a hollow structure, and the aperture of the first installation groove 31052 is larger than the aperture of the hollow structure, so that when the first heating part 3104 is installed in the heat insulation part 3105, it will not directly contact the top plate 36, preventing heat from diffusing towards the middle and bottom of the test tube; two adjacent heat insulation parts 3105 are connected by a first connecting part 31053. The first connecting part 31053 includes a second base 310531 and a second installation groove 310532. The second base 310531 is connected to the first base 31051, the second installation groove 310532 is communicated with the first installation groove 31052, and the second installation groove 310532 is strip-shaped. It can be understood that the heat insulation part 3105 and the first connecting part 31053 in this embodiment can be integrally formed or can be formed by an assembled structure; similarly, two adjacent first heating parts 3104 are connected by a second connecting part 37 (as Figure 11 shown), during installation, the first heating part 3104 is installed in the first installation groove 31052, and the second connecting part 37 is installed in the second installation groove 310532.

[0088] It should be noted that the top plate 36 and the chassis 35 in this embodiment are preferably in a disc-shaped structure to be adapted to the disc-shaped structure formed by the test tube installation assembly 31 and the gear disc 34.

[0089] Please refer to Figure 4 、 Figure 13 and Figure 14The amplification device 2 includes a heating disk 21, a second heating element 22 and a heat-conducting element 23. A third through hole 2101 is provided on the heating disk 21, so that the output shaft of the first driving element 32 passes through the third through hole 2101 of the heating disk 21 and is connected to the extraction device 3. The second heating element 22 is installed on the heating disk 21 and is located below the extraction device 3 to heat the bottom of the test tube; the heat-conducting element 23 and the first driving element 32 are arranged on the side of the heating disk 21 away from the second heating element 22, and the heat-conducting element 23 is used for heat conduction and cooling to achieve PCR (Polymerase Chain Reaction) amplification.

[0090] In some embodiments, there are multiple second heating elements 22 , and the multiple second heating elements 22 are spliced around the axis of the heating plate 21 to form an annular structure.

[0091] It can be understood that the heating plate 21 is a disc-shaped structure, which is compatible with the structure of the bottom plate 35; the second heating element 22 in this embodiment is an equal-sized structure, and the annular structure formed by splicing multiple second heating elements 22 is compatible with the annular arrangement structure of the test tube installation assembly 31, so as to heat the bottom of the test tube more concentratedly. The heating plate 21 in this embodiment extends in a direction toward one side of the bottom plate 35 to form a side wall, which can prevent heat from dissipating to a certain extent.

[0092] See also Figure 15 and Figure 16 , and further includes a cooling fan 6, which is mounted on the bracket 1, and the air intake of the cooling fan 6 faces the amplification device 2, and is used to absorb heat and blow it out of the device to improve the cooling capacity of the amplification device 2. The cooling fan 6 cooperates with the second heating element 22 and the heat-conducting element 23 to achieve PCR amplification. It can be understood that a heat dissipation port is provided on the bracket 1, and the heat dissipation port is located below the amplification device 2, and is used for the cooling fan 6 to absorb the heat derived from the heat-conducting element 23 through the heat dissipation port for heat dissipation.

[0093] In practical applications, one, two, three or more cooling fans 6 may be provided according to actual needs.

[0094] It should be noted that the amplification process in this embodiment is a process of continuous heating and cooling cycles, about 30-50 heating and cooling cycles, and fluorescence collection is required for each cycle, that is, there are 30-50 amplification cycles and fluorescence collection is also 30-50 times.

[0095] See also Figure 2 and Figure 5, a light guiding device 7 is arranged between the extraction device 3 and the amplification device 2. The light guiding device 7 has a fifth mounting hole 71 and a first light guiding hole 72. The fifth mounting hole 71 is correspondingly arranged with the third mounting hole 3501, and the bottom of the test tube extends into the light guiding device 7 through the fifth mounting hole 71. The light emitted by the fluorescence detection device 4 irradiates the bottom of the test tube through the light guiding device 7 and the first light guiding hole 72, realizing the fluorescence detection and collection of nucleic acids. It can be understood that the light guiding device 7 in this embodiment can not only play a role in guiding light, but also, when the bottom of the test tube extends into the light guiding device 7, it can play a certain role in shielding light to prevent the influence of external light.

[0096] Please refer to Figure 6 and Figure 17 , the light guiding device 7 includes a light guiding disc 73 and a light guiding column 74. The side surface of the light guiding disc 73 facing the fluorescence detection device 4 is provided with a first light guiding hole 72 for the light emitted by the fluorescence detection device 4 to pass through. The side of the light guiding disc 73 facing the extraction device 3 is provided with a fifth mounting hole 71. The light guiding column 74 is arranged in the light guiding disc 73 and is located between the first light guiding hole 72 and the fifth mounting hole 71, and is used to irradiate the bottom of the test tube with the light emitted by the fluorescence detection device 4 through the first light guiding hole 72.

[0097] Refer to together Figure 6 , in this embodiment, the light guiding disc 73 includes a first disc 7302 and a second disc 7303. The first disc 7302 and the second disc 7303 are connected to form an installation cavity. The first disc 7302 extends along the direction towards the second disc 7303 to form a support wall, and the first light guiding hole 72 is opened on this support wall. The light guiding column 74 is installed on the second disc 7303. When the fluorescence detection device 4 starts to work, the light it emits enters from the first light guiding hole 72 and is directly irradiated on the bottom of the test tube through the transmission of the light guiding column 74, realizing fluorescence collection and feeding back the collected information to the fluorescence detection device 4 to complete the fluorescence detection of nucleic acids.

[0098] In some embodiments, the first light guiding hole 72 and the light guiding column 74 form an optical path for the light emitted by the fluorescence detection device 4 to pass through. The center line of the first light guiding hole 72 and the center line of the fifth mounting hole 71 are arranged at a certain angle so that the light incident from the side can irradiate the bottom of the test tube; preferably, the center line of the first light guiding hole 72 is perpendicular to the center line of the fifth mounting hole 71.

[0099] As Figure 4 and Figure 5 shown, the light guiding disc 73 further includes a light guiding tube 7301. The light guiding tube 7301 is arranged on the second disc 7303, and one end of the light guiding tube 7301 close to the first light guiding hole 72 has a second light guiding hole, which is communicated with the first light guiding hole 72. One end of the light guiding tube 7301 close to the fifth mounting hole 71 has a light guiding part 73011 (as Figure 17As shown in the figure, the light guide portion 73011 extends into the fifth mounting hole 71, and the light guide portion 73011 has a through hole to achieve communication with the fifth mounting hole 71. The light guide column 74 is arranged in the light guide tube 7301 to converge the light emitted by the fluorescence detection device 4 in the light guide tube 7301 and directly irradiate the bottom of the test tube through the light guide column 74, improving the detection accuracy.

[0100] As Figure 17 shown, in some embodiments, there are multiple first light guide holes 72, and the multiple first light guide holes 72 are arranged at intervals around the axis of the light guide disc 73 on the support wall; there are multiple fifth mounting holes 71, and the multiple fifth mounting holes 71 are arranged at intervals around the axis of the light guide disc 73. It can be understood that the multiple fifth mounting holes 71 in this embodiment are arranged in an annular layout on the first disc 7302, and are arranged according to the position of the fitting 3103 to achieve one fifth mounting hole 71 corresponding to one third mounting hole 3501 to realize the fluorescence detection of nucleic acid in the test tube. At this time, the distances between the fifth mounting holes 71 and the center of the first disc 7302 are different. The fifth mounting hole 71 corresponding to the third mounting hole 3501 located in the inner circle is defined as R1, and its distance from the center of the first disc 7302 is shorter; the fifth mounting hole 71 corresponding to the third mounting hole 3501 located in the outer circle is positioned as R2, and its distance from the center of the first disc 7302 is longer; R1 and R2 are arranged in a cross pattern, that is, there is an R2 between every two adjacent R1s, and they are arranged in one-to-one correspondence with the positions of the third mounting holes 3501. Of course, in some embodiments, if there is only one circle of the fitting 3103, the distances between the fifth mounting holes 71 and the center of the first disc 7302 are equal at this time.

[0101] It should be noted that the first light guide holes 72 and the fifth mounting holes 71 are arranged in one-to-one correspondence, and a light guide column 74 is arranged between each first light guide hole 72 and the corresponding fifth mounting hole 71.

[0102] As Figure 18 shown, in some embodiments, the fluorescence detection device 4 includes a transmitting end; the diameter of the light guide disc 73 is smaller than the diameter of the extraction device 3, and the diameter of the light guide disc 73 is smaller than the diameter of the amplification device 2. A groove structure is defined between the extraction device 3, the light guide disc 73 and the amplification device 2, and the transmitting end is located in the groove structure. During the process of emitting light from the transmitting end, the groove structure can achieve a light shielding effect, so that the light path will not be interfered by external light, preventing the external light source from having a greater impact on the light emission and reception of the fluorescence detection device 4, avoiding affecting the detection result, and making the detection structure more accurate.

[0103] Refer to together Figure 2, the fluorescence detection device 4 includes a light emission component 41, an optical fiber 42, and an optical fiber transmitter 43. The light emission component 41 is installed on the bracket 1, and the optical fiber transmitter 43 is installed on the bracket 1 through a mounting base. The optical fiber transmitter 43 has a transmitting end, which can also be used as a receiving end for receiving the detection results fed back from the test tube. The light emission component 41 and the optical fiber transmitter 43 are connected by two optical fibers 42. One optical fiber 42 is used to connect the transmitting end of the light emission component 41 and the transmitting end of the optical fiber transmitter 43, and the other optical fiber 42 is used to connect the receiving end of the light emission component 41 and the receiving end of the optical fiber transmitter 43. The light emitted from the transmitting end of the optical fiber transmitter 43 irradiates the bottom of the test tube through the first light guide hole 72 and the light guide column 74.

[0104] As Figure 2 shown, in some embodiments, there are multiple light emission components 41, and the multiple light emission components 41 are arranged at intervals around the output shaft of the driving device 5; there are multiple optical fiber transmitters 43, and the multiple optical fiber transmitters 43 are arranged at intervals around the axis of the bracket 1.

[0105] In this embodiment, there are 4 light emission components 41 located at the four corners of the bracket 1. Similarly, there are 4 optical fiber transmitters 43, and the optical fiber transmitters 43 and the light emission components 41 are arranged in an interleaved manner, that is, one optical fiber transmitter 43 is arranged between two adjacent light emission components 41. By arranging the optical fiber transmitters 43 at intervals between the light emission components 41, the structure can be made more compact without occupying more space.

[0106] As Figure 2 shown, the fluorescence detection device 4 further includes a driving component 44. The light emission component 41 includes multiple light sources, multiple kinds of optical filters, and a first transmission member 4101. The first transmission member 4101 has a transmission end connected to the driving component 44 and a mounting end located on the outgoing light path. The multiple light sources are installed at intervals around the axis of the mounting end, and an optical filter is correspondingly arranged at the front end of the emission head of each light source.

[0107] Understandably, the optical emission component 41 in this embodiment has different optical filters. Each light source and the corresponding optical filter cooperate in pairs to achieve the effect of outputting and receiving light in a preset wavelength band. Preferably, there are 5 optical filters, and the light source can emit light in five wavelength bands through the optical filters. The installation end of the first transmission member 4101 in this embodiment is a fan-shaped structure, and 5 light sources are arranged in sequence along the structural shape of the installation end. The driving component 44 drives the first transmission member 4101 to rotate. Whenever the driving component 44 drives the first transmission member 4101 to rotate once, the first transmission member 4101 drives a light source to rotate to the light output path to emit light of a corresponding wavelength. The light source emits monochromatic light through the optical filter, and the monochromatic light is transmitted to the optical fiber transmitter 43 through the optical fiber 42, and is irradiated on the bottom of the test tube through the first light guide hole 72 and the light guide column 74 for fluorescence collection. The driving component 44 drives the first transmission member 4101 to switch different light sources and optical filters, and sequentially emits light sources of different colors until the fluorescence of the test tube is collected five times.

[0108] Understandably, the light source in this embodiment can be a laser or other types of light sources, which are not particularly limited herein as long as they can cooperate with the corresponding optical filters in pairs to achieve the effect of outputting and receiving light in a preset wavelength band.

[0109] As Figure 2 shown, in some embodiments, the driving component 44 includes a second driving member 4401 and a conveyor belt 4402. The first transmission member 4101 and the second driving member 4401 are synchronously driven through the conveyor belt 4402. In this embodiment, the conveyor belt 4402 is sequentially connected to the transmission end of the first transmission member 4101, and the second driving member 4401 drives a plurality of first transmission members 4101 to simultaneously switch different monochromatic lights, improving efficiency.

[0110] As Figure 2 shown, the driving device 5 includes a third driving member 51, a timing belt 52, and a second transmission member 53. The third driving member 51 is installed on the bracket 1, and the second transmission member 53 is installed at the bottom of the amplification device 2. The third driving member 51 and the second transmission member 53 are synchronously driven through the timing belt 52 to drive the amplification device 2 to rotate. Understandably, the second transmission member 53 is sleeved on the output shaft of the first driving member 32, and the two do not contact.

[0111] After the fluorescence of the test tube is collected five times, the third driving member 51 drives the timing belt 52 to drive the second transmission member 53 to rotate, thereby driving the amplification device 2 and the light guide device 7 located on the amplification device 2 to rotate, so that the next first light guide hole 72 is aligned with the emission end of the optical fiber transmitter 43, and the above-mentioned five-color fluorescence collection is performed again. Rotate 8 times in sequence, and rotate about 90° in total to complete the fluorescence collection of the bottoms of all test tubes on the extraction device 3.

[0112] The device provided in this embodiment can simultaneously place 32 samples into the device and finally output results together. Without manual detection, it improves the detection efficiency, shortens the detection time, and realizes single-module multi-flux in terms of structure. Standard control and blank control under the same condition can be added to the 32 samples, which can meet the usage requirements, further improve the efficiency and accuracy of this device, and also improve the detection throughput as a screening reagent.

[0113] Refer to together Figure 15 , the bracket 1 includes an upper bracket 11 and a lower bracket 12. The upper bracket 11 and the lower bracket 12 are connected by fasteners. The upper bracket 11 and the lower bracket 12 serve as a bearing platform, providing an installation space for the installation of the amplification device 2, the extraction device 3, the fluorescence detection device 4, the driving device 5, and the cooling fan 6. A space is formed between the upper bracket 11 and the lower bracket 12. The first driving member 32 and the third driving member 51 are located in this space. The first driving member 32, the third driving member 51, the amplification device 2, and the fluorescence detection device 4 are installed on the upper bracket 11, and the cooling fan 6 is installed on the lower bracket 12.

[0114] It can be understood that bolt holes are opened at corresponding positions on the upper bracket 11 and the lower bracket 12, and the two are connected by bolts. In addition, hollow mounting posts can also be provided on the lower bracket 12, and the bolts pass through the bolt holes on the upper bracket 11 and are connected to the mounting posts. Here, the specific connection method is not limited.

[0115] In summary, the embodiment of the present invention provides a nucleic acid extraction and amplification integrated detection device, which is small in volume and convenient to carry. It can not only reduce the difficulty of laboratory construction and operation, but also solve the problem of aerosol pollution easily generated in the laboratory, improve safety, and at the same time can complete steps such as nucleic acid extraction, amplification, and detection with a single device product, integrating multiple functions.

[0116] It should be understood that in the present invention, terms such as "first" and "second" are used to describe various information, but these information should not be limited to these terms. These terms are only used to distinguish the same type of information from each other. For example, without departing from the scope of the present invention, "first" information can also be called "second" information, and similarly, "second" information can also be called "first" information. In addition, the orientation or positional relationship indicated by terms such as "upper", "lower", "left", and "right" is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.

[0117] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the technical principle of the present invention, several improvements and substitutions can be made, and these improvements and substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A nucleic acid extraction and amplification integrated detection device, characterized in that, Comprising: A bracket (1); An amplification device (2), arranged on the bracket (1) for temperature regulation; An extraction device (3), mounted on the amplification device (2), the extraction device (3) includes a test tube mounting assembly (31), a first driving member (32) and a magnetic attracting member (33), the test tube mounting assembly (31) has a first mounting hole (3101), the magnetic attracting member (33) is arranged on the inner wall of the first mounting hole (3101), the first driving member (32) is mounted on the bracket (1) for driving the test tube mounting assembly (31) to rotate, so as to drive the magnetic attracting member (33) to rotate around the outer peripheral side of the test tube located in the test tube mounting assembly (31) and perform nucleic acid extraction; A fluorescence detection device (4), mounted on the bracket (1) for performing fluorescence detection on the test tube; And A driving device (5), mounted on the bracket (1), and the output end of the driving device (5) is connected to the amplification device (2) for driving the amplification device (2) and the extraction device (3) to rotate relative to the fluorescence detection device (4).

2. The nucleic acid extraction and amplification integrated detection device according to claim 1, characterized in that, The extraction device (3) further includes a gear disk (34), the test tube mounting assembly (31) includes a gear member (3102), and the output shaft of the first driving member (32) passes through the amplification device (2) and is connected to the gear disk (34); The gear member (3102) has the first mounting hole (3101), the gear disk (34) meshes with the gear member (3102), and the magnetic attracting member (33) is arranged on the gear member (3102).

3. The nucleic acid extraction and amplification integrated detection device according to claim 2, wherein The first mounting hole (3101) penetrates through both ends of the gear member (3102), and a second mounting hole (31021) is formed on the inner wall of the first mounting hole (3101), and the magnetic attracting member (33) is arranged in the second mounting hole (31021).

4. The nucleic acid extraction and amplification integrated detection device according to claim 2, wherein The extraction device (3) further includes a chassis (35) and a top plate (36), a receiving cavity is formed between the chassis (35) and the top plate (36), the gear disk (34) is arranged in the receiving cavity, the chassis (35) is arranged on the amplification device (2), and a third mounting hole (3501) for the bottom of the test tube to pass through is arranged on the chassis (35).

5. The nucleic acid extraction and amplification integrated detection device according to claim 4, wherein On one side surface of the gear member (3102) facing the chassis (35) and on one side surface facing the top plate (36), fourth mounting holes (31022) are provided, and balls are arranged in the fourth mounting holes (31022); The test tube mounting assembly (31) further includes a fitting (3103), the fitting (3103) is arranged on the chassis (35), a first sliding groove (31031) is formed on one side surface of the fitting (3103) facing the top plate (36), and the third mounting hole (3501) penetrates through the first sliding groove (31031); a second sliding groove (3601) corresponding to the gear member (3102) is formed on one side surface of the top plate (36) facing the chassis (35); The gear component (3102) is arranged between the top plate (36) and the matching component (3103), the gear component (3102) and the matching component (3103) are arranged correspondingly, and the gear component (3102) can rotate on the first slide groove (31031) and the second slide groove (3601) through the ball bearing.

6. The nucleic acid extraction and amplification integrated detection device according to claim 4, wherein The test tube mounting assembly (31) further comprises a first heating element (3104), wherein the first heating element (3104) is disposed on the top plate (36) and is arranged corresponding to the gear element (3102).

7. The nucleic acid extraction and amplification integrated detection device according to claim 6, wherein, The test tube installation assembly (31) further comprises a heat insulating member (3105), wherein the heat insulating member (3105) is sleeved on the outer peripheral side of the first heating member (3104).

8. The nucleic acid extraction and amplification integrated detection device according to any one of claims 2-7, characterized in that, There are multiple test tube installation components (31), and the multiple test tube installation components (31) are arranged around the circumference of the gear plate (34) to form a test tube installation group; The number of the test tube installation groups is n, and the n test tube installation groups are arranged in sequence along the radial direction of the gear plate (34), wherein n≥1.

9. The nucleic acid extraction and amplification integrated detection device according to claim 1, wherein, The amplification device (2) comprises a heating plate (21), a second heating element (22) and a heat-conducting element (23); the second heating element (22) is arranged on the heating plate (21) and is located below the extraction device (3); the heat-conducting element (23) and the first driving element (32) are arranged on a side of the heating plate (21) away from the second heating element (22).

10. The nucleic acid extraction and amplification integrated detection device according to claim 9, characterized in that, The number of the second heating elements (22) is plural, and the plurality of second heating elements (22) are spliced around the axis of the heating plate (21) to form an annular structure.

11. The nucleic acid extraction and amplification integrated detection device according to claim 1, wherein, It also includes a cooling fan (6), which is installed on the bracket (1), and the air intake of the cooling fan (6) faces the amplification device (2).

12. The nucleic acid extraction and amplification integrated detection device according to claim 4, wherein A light guide device (7) is arranged between the extraction device (3) and the amplification device (2), and the light guide device (7) has a fifth mounting hole (71) and a first light guide hole (72). The fifth mounting hole (71) is arranged corresponding to the third mounting hole (3501), and the bottom of the test tube extends into the light guide device (7) through the fifth mounting hole (71), and the light emitted by the fluorescence detection device (4) is irradiated onto the bottom of the test tube through the first light guide hole (72).

13. The nucleic acid extraction and amplification integrated detection device according to claim 12, wherein The light guide device (7) comprises a light guide disc (73) and a light guide column (74); the light guide disc (73) is provided with the first light guide hole (72) on the side facing the fluorescence detection device (4) for light emitted by the fluorescence detection device (4) to pass through; the light guide disc (73) is provided with the fifth mounting hole (71) on the side facing the extraction device (3); the light guide column (74) is arranged in the light guide disc (73) and is located between the first light guide hole (72) and the fifth mounting hole (71), and is used for irradiating the light emitted by the fluorescence detection device (4) to the bottom of the test tube through the first light guide hole (72).

14. The nucleic acid extraction and amplification integrated detection device according to claim 13, characterized in that, The central axis of the first light guide hole (72) is perpendicular to the central axis of the fifth mounting hole (71).

15. The nucleic acid extraction and amplification integrated detection device according to claim 14, characterized in that, There are multiple first light guide holes (72), and the multiple first light guide holes (72) are arranged at intervals around the axis of the light guide disc (73); There are multiple fifth mounting holes (71), and the multiple fifth mounting holes (71) are arranged at intervals around the axis of the light guide disc (73). The first light guide holes (72) and the fifth mounting holes (71) are arranged in one-to-one correspondence; A light guide column (74) is arranged between each first light guide hole (72) and the corresponding fifth mounting hole (71).

16. The nucleic acid extraction and amplification integrated detection device according to any one of claims 13-15, characterized in that, The light guide disc (73) further includes a light guide tube (7301). One end of the light guide tube (7301) is communicated with the first light guide hole (72), the other end of the light guide tube (7301) is communicated with the fifth mounting hole (71), and the light guide column (74) is arranged in the light guide tube (7301).

17. The nucleic acid extraction and amplification integrated detection device according to claim 13, wherein The fluorescence detection device (4) includes a transmitting end; The diameter of the light guide disc (73) is smaller than the diameter of the extraction device (3), and the diameter of the light guide disc (73) is smaller than the diameter of the amplification device (2). A groove structure is defined between the extraction device (3), the light guide disc (73) and the amplification device (2), and the transmitting end is located in the groove structure.

18. The nucleic acid extraction and amplification integrated detection device according to claim 17, wherein, The fluorescence detection device (4) includes a light emitting component (41), an optical fiber (42) and an optical fiber transmitter (43). The light emitting component (41) and the optical fiber transmitter (43) are installed on the bracket (1). The light emitting component (41) and the optical fiber transmitter (43) are connected by the optical fiber (42). The optical fiber transmitter (43) has the transmitting end, and the light emitted from the transmitting end irradiates the bottom of the test tube through the first light guide hole (72) and the light guide column (74).

19. The nucleic acid extraction and amplification integrated detection device according to claim 18, characterized in that, There are multiple light emitting components (41), and the multiple light emitting components (41) are arranged at intervals around the output shaft of the driving device (5); There are multiple optical fiber transmitters (43), and the multiple optical fiber transmitters (43) are arranged at intervals around the axis of the bracket (1).

20. The nucleic acid extraction and amplification integrated detection device according to claim 18 or 19, characterized in that, The fluorescence detection device (4) further includes a driving component (44). The light emitting component (41) includes multiple light sources, multiple kinds of filter plates and a first transmission member (4101). The first transmission member (4101) has a transmission end connected to the driving component (44) and a mounting end located on the outgoing light path. The multiple light sources are installed at intervals around the axis of the mounting end, and a filter plate is correspondingly arranged at the front end of the emitting head of each light source; Whenever the driving component (44) drives the first transmission member (4101) to rotate once, the first transmission member (4101) drives one light source to rotate to the outgoing light path to emit light with a corresponding wavelength.

21. The nucleic acid extraction and amplification integrated detection device according to claim 20, wherein The driving component (44) includes a second driving member (4401) and a conveyor belt (4402), and the first transmission member (4101) and the second driving member (4401) are synchronously driven through the conveyor belt (4402).

22. The nucleic acid extraction and amplification integrated detection device according to claim 1, wherein The driving device (5) includes a third driving member (51), a timing belt (52), and a second transmission member (53). The third driving member (51) is installed on the bracket (1), and the second transmission member (53) is installed at the bottom of the amplification device (2). The third driving member (51) and the second transmission member (53) are synchronously driven through the timing belt (52) to drive the amplification device (2) to rotate.

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