Fermented lactobacillus mucus capable of prolonging life, resisting aging and resisting oxidation as well as product and application of fermented lactobacillus mucus

Abstract

The invention discloses application of fermented lactobacillus mucus capable of prolonging life, resisting aging and resisting oxidation, and belongs to the technical field of functional strains. The preservation numbers of the lactobacillus mucus A21224 and the lactobacillus mucus A21227 are GDMCC No: 62958 and GDMCC No: 62962 respectively, and the lactobacillus mucus A21224, the lactobacillus mucus A21227 and the lactobacillus mucus A21227 are used for The two strains can remarkably prolong the service life of nematodes, have good antioxidant effect and heat stress resistant effect, can prolong the service life of the nematodes by 40% in a strong oxidation environment, and can prolong the service life of the nematodes by 28.57% under the stress of a thermal environment. In addition, the lactobacillus mucus A21224 and the lactobacillus mucus A21227 both show an excellent anti-aging effect in the late stage of the nematodes. Meanwhile, the two strains have certain gastrointestinal fluid-resistant and cholate-resistant effects, can be colonized in intestinal tracts, have no hemolysis phenomenon and are high in safety.

Description

Technical Field

[0001] The present invention relates to the technical field of functional strains, and particularly relates to a Limosilactobacillus fermentum with life extension, anti-aging, and antioxidant effects, and its products and applications. Background Art

[0002] In 2011, Limosilactobacillus fermentum (also known as Lactobacillus fermentum) was included in the List of Strains Allowed to be Used in Foods in China. According to existing reports, Limosilactobacillus fermentum can increase the number of beneficial bacteria in the intestine and maintain the balance of the intestinal flora. At the same time, Limosilactobacillus fermentum can also produce lactic acid and other beneficial substances, reduce the pH value in the intestine, and maintain the acid-base balance of the intestine, thereby promoting intestinal health. Limosilactobacillus fermentum can also activate the immune system and enhance the body's immunity.

[0003] In recent years, many scholars have found that Limosilactobacillus fermentum can decompose complex carbohydrates, proteins, and fats in food, promote the digestion and absorption of food, and can also promote intestinal peristalsis, increase the volume and moisture of feces, and relieve symptoms of indigestion such as constipation. In addition, some scholars have also found that Limosilactobacillus fermentum can decompose cholesterol and fatty acids, reduce the absorption and synthesis of cholesterol, thereby reducing the cholesterol level in the blood and preventing the occurrence of cardiovascular diseases.

[0004] In order to develop strains with more functions, the Caenorhabditis elegans experimental model has been widely used. Caenorhabditis elegans is a non-toxic and harmless nematode that can survive independently. Due to its clear genetic background, simple individual structure, short life cycle, and completed genome sequencing, it has been widely used in the fields of genetics and developmental biology, behavior and neurobiology, aging and lifespan, human genetic diseases, the interaction between pathogens and organisms, drug screening, the stress response of animals, environmental biology, and signal transduction.

[0005] The present invention provides the effects of a strain of Limosilactobacillus fermentum in models such as nematode lifespan, aging, and environmental stress, providing data support for subsequent product development. Summary of the Invention

[0006] The present invention aims to at least solve the above technical problems in the prior art. To this end, the object of the present invention is to provide a Limosilactobacillus fermentum with life extension, anti-aging, and antioxidant effects, and its products and applications.

[0007] To achieve the above object, the technical solutions adopted by the present invention are as follows:

[0008] The first aspect of the present invention provides a fermented mucus lactobacillus, which is fermented mucus lactobacillus A21224, taxonomically named Limosilactobacillus fermentum, and is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on November 11, 2022 (the preservation address is: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou), and the preservation number is GDMCC No: 62958.

[0009] In some embodiments of the present invention, the colony morphology of the fermented mucus Lactobacillus A21224 is a round, convex, and entire colony.

[0010] In some embodiments of the present invention, the 16S sequence of the fermentative Lactobacillus mucilaginosus A21224 is shown as SEQ ID NO:3.

[0011] In some embodiments of the present invention, the screening and isolation method of the fermented mucus Lactobacillus A21224 is: taking a stool sample from a centenarian in Guangxi Zhuang Autonomous Region to an MRS solid culture medium, smearing it evenly, placing it in an anaerobic workstation and culturing it for two days, picking colonies with round, convex, entire and colorless colonies in the plate and culturing them in a small amount of MRS liquid culture medium, and after PCR amplification and identification, the fermented mucus Lactobacillus A21224 is obtained.

[0012] In some embodiments of the present invention, the culture conditions in the anaerobic workstation are 37° C., 90% nitrogen + 10% carbon dioxide.

[0013] In some embodiments of the present invention, the primers used for PCR amplification and identification are shown as SEQ ID NO: 1 and SEQ ID NO: 2.

[0014] In some embodiments of the present invention, the amplification system for PCR amplification identification is:

[0015] Component Content 10 μM forward primer 16S-F 1 μL 10 μM reverse primer 16S-R 1 μL PCR amplification solution 12.5 μL DNA template 1 μL <![CDATA[ddH2O]]> Make up to 25 μL.

[0016] In some embodiments of the present invention, the amplification program of the PCR amplification identification is 95°C for 3 min; 95°C for 40 s, 56°C for 30 s, 72°C for 1 min, 30 cycles; 72°C for 8 min.

[0017] The second aspect of the present invention provides a culture of fermented Lactobacillus mucilaginosus A21224.

[0018] In some embodiments of the present invention, the culture comprises the supernatant obtained after culturing Lactobacillus mucosae A21224.

[0019] In some embodiments of the present invention, the culture comprises the metabolites of Lactobacillus mucosae A21224.

[0020] The third aspect of the present invention provides a bacterial agent.

[0021] In some embodiments of the present invention, the bacterial agent comprises Lactobacillus mucosae A21224 described in the above aspect and / or the culture of Lactobacillus mucosae A21224.

[0022] In some embodiments of the present invention, the bacterial agent is a solid bacterial agent or a liquid bacterial agent.

[0023] The fourth aspect of the present invention provides the use of any one or more of Lactobacillus mucosae, the culture of Lactobacillus mucosae, and / or the bacterial agent containing Lactobacillus mucosae in at least one of the following (I) to (III);

[0024] (I) Preparing a product for extending lifespan;

[0025] (II) Preparing an anti-aging product;

[0026] (III) Preparing an antioxidant product.

[0027] In some embodiments of the present invention, the Lactobacillus mucosae is Lactobacillus mucosae A21224 and / or Lactobacillus mucosae A21227 described in the above aspect; Lactobacillus mucosae A21227 is published in a Chinese patent with the patent number CN115927116A.

[0028] In some embodiments of the present invention, the culture of Lactobacillus mucosae comprises the metabolites of Lactobacillus mucosae.

[0029] The fifth aspect of the present invention provides a product containing any one or more of Lactobacillus mucosae A21224, the culture of Lactobacillus mucosae A21224, and the bacterial agent described in the above aspect.

[0030] In some embodiments of the present invention, the product includes food, food additives, feed, feed additives, drugs, and cosmetics.

[0031] In some embodiments of the present invention, the product further contains excipients.

[0032] In some embodiments of the present invention, the excipients include food additives, pharmaceutically acceptable adjuvants, or cosmetic excipients.

[0033] In some embodiments of the present invention, the forms of Lactobacillus mucosae fermentum in the product include freeze-dried powder, bacterial liquid, and granular inoculant.

[0034] In some embodiments of the present invention, the dosage forms of the drug include powder, granule, capsule, tablet, pill, or oral liquid.

[0035] The beneficial effects of the present invention are as follows:

[0036] The present invention provides a Lactobacillus mucosae fermentum A21224 and A21227 with life extension, anti-aging, and antioxidant effects, as well as their products and applications. Both Lactobacillus mucosae fermentum A21224 and A21227 can significantly increase the lifespan of nematodes, extending the lifespan of nematodes by 13.33% and 20% respectively in the experiment. Both have good antioxidant effects and anti-thermal stress effects. In a strongly oxidative environment, both strains of bacteria can increase the lifespan of nematodes by 40%. Under heat stress, they can increase the lifespan of nematodes by 28.57%. In addition, Lactobacillus mucosae fermentum A21224 and A21227 both show excellent anti-aging effects in the late stage of nematode life. At the same time, both strains of bacteria have certain effects of resisting gastrointestinal fluid and bile salt, can colonize in the intestine, and neither strain has hemolysis phenomenon, with high safety. Description of the Drawings

[0037] Figure 1 It is an evolutionary tree of Lactobacillus mucosae fermentum A21224 and A21227.

[0038] Figure 2 It is the effect of Lactobacillus mucosae fermentum A21224 and A21227 on the lifespan of nematodes.

[0039] Figure 3 It is the antioxidant effect of Lactobacillus mucosae fermentum A21224 and A21227.

[0040] Figure 4 It is the anti-thermal stress effect of Lactobacillus mucosae fermentum A21224 and A21227 on nematodes.

[0041] Figure 5 It is the effect of Lactobacillus mucosae fermentum A21224 and A21227 on the head swing of nematodes on the 15th day.

[0042] Figure 6 It is the effect of Lactobacillus mucosae fermentum A21224 and A21227 on the body bend of nematodes on the 15th day.

[0043] Figure 7 It is the hemolysis experiment effect of Lactobacillus mucosae fermentum A21224.

[0044] Figure 8 For the hemolysis test results of Lactobacillus mucosae A21227 Specific implementation mode

[0045] The content of the present invention will be further described in detail through specific embodiments below. The raw materials, reagents or devices used in the examples and comparative examples can be obtained from conventional commercial channels or can be obtained by existing technical methods without special instructions. Unless otherwise specified, the test or test methods are all conventional methods in the art.

[0046] Example 1 Isolation and purification of Lactobacillus mucosae A21224

[0047] This example provides a method for the isolation and purification of Lactobacillus mucosae A21224. The specific experimental steps are as follows.

[0048] (1) Take the fecal samples of centenarians in Guangxi Zhuang Autonomous Region. Take an appropriate amount of fecal samples into MRS solid medium, smear evenly, and then place them in an anaerobic workstation and culture for two days under the conditions of 37 °C, 90% nitrogen + 10% carbon dioxide;

[0049] (2) After the culture is completed, pick at least a small amount of colonies with round, convex and entire colony morphology on the plate into MRS liquid medium (peptone 10 g / L, beef extract powder 5 g / L, yeast extract powder 4 g / L, glucose 20 g / L, dipotassium hydrogen phosphate 2 g / L, ammonium citrate 2 g / L, sodium acetate 5 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, agar 15 g / L, Tween-80 1 g / L. After mixing the above components, add 1 L of deionized water, adjust the pH value to 6.2 ± 0.2, and sterilize at 121 °C for 15 min to obtain it), mix evenly and transfer to a PCR tube, and continue to culture in a 37 °C incubator for 24 h;

[0050] (3) Add PCR amplification solution (2×Taq PCR StarMix with Loading Dye, Beijing Kangrun Chengye Biotechnology Co., Ltd.) to the cultured PCR tube, and add 16S primers for PCR amplification (the amplification system is shown in Table 1 below);

[0051] Among them, the primers for 16S are:

[0052] Upstream primer 16S-F: 5’-AGAGTTTGATCCTGGCTCAG-3’ (SEQ ID NO:1);

[0053] Downstream primer 16S-R: 5’-TACGGCTACCTTGTTACGACTT-3’ (SEQ ID NO:2);

[0054] Table 1 PCR amplification system for Lactobacillus mucosae fermentum

[0055]

[0056]

[0057] The amplification procedure was as follows: 95°C for 3 min; 95°C for 40 s, 56°C for 30 s, 72°C for 1 min, for 30 cycles; 72°C for 8 min;

[0058] The amplified product was sent to Beijing Tsingke Biotechnology Co., Ltd. for sequencing and identification.

[0059]

[0060] (4) The confirmed culture medium was purified by streak plate method twice. Single colonies after purification were picked for repeated culture to obtain bacterial liquid. The bacterial liquid was mixed with 40% glycerol preservation solution at a volume ratio of 1:1 in a strain cryopreservation tube and stored in a -80 °C refrigerator.

[0061] (5) The 16S sequences obtained in (3) were used to construct a phylogenetic tree by the neighbor-joining method on the MEGA-X 10.2.2 software (https: / / www.megasoftware.net / ).

[0062] The construction result of the phylogenetic tree is as Figure 1 shown. It was confirmed that the strain isolated and purified in this example was Limosilactobacillus fermentum, and it was named Limosilactobacillus fermentum A21224.

[0063] The obtained Limosilactobacillus fermentum A21224 was sent to the Guangdong Provincial Culture Collection of Microorganisms (GDMCC) for preservation. Its taxonomic name is: Limosilactobacillus fermentum. The preservation date is: November 11, 2022. The preservation address is: 5th Floor, Building 59, No. 100 Compound, Xianlie Middle Road, Guangzhou. The preservation number is: GDMCC No:62958.

[0064] Example 2 Effects of Limosilactobacillus fermentum A21224 and A21227 on the lifespan of Caenorhabditis elegans

[0065] This example provides a test on the effects of Limosilactobacillus fermentum A21224 and A21227 on the lifespan of Caenorhabditis elegans. Limosilactobacillus fermentum A21227 was published in a Chinese patent with the patent number CN115927116A. The specific experimental steps are as follows.

[0066] (1) Limosilactobacillus fermentum A21224 and A21227 were cultured in a conventional MRS liquid medium and incubated in a shaker at 37 °C for 48 hours.

[0067] (2) Escherichia coli OP50 was cultured in an LB liquid medium under the following conditions: temperature 37 °C, time 20 hours, rotation speed 200 rpm.

[0068] (3) Bacterial liquids of Limosilactobacillus fermentum A21224 and A21227 and Escherichia coli OP50 were respectively spread on nematode growth medium (NGM) plates and cultured overnight.

[0069] The formula of the NGM medium is as follows: 3 g of sodium chloride, 17 g of agar powder, 2.5 g of peptone, 975 mL of deionized water. After thoroughly mixing the above components, seal it with tin foil. After autoclaving at high pressure for 20 min, cool it in a water bath to 55 °C. Under sterile conditions, add the following sterilized solutions: 1 mL of 1 M CaCl2, 1 mL of 1 M MgSO4, 25 mL of 1 M KPO4 buffer (108.3 g of KH2PO4 and 35.6 g of K2HPO4, dissolved in 1000 mL of distilled water, adjust the pH value to 6.0), 1 mL of 5 mg / mL cholesterol (dissolved in 95% ethanol);

[0070] (4) After centrifuging the thawed wild-type nematodes (Caenorhabditis elegans, kindly provided by Researcher Bin Wang of Guangxi Academy of Sciences), add them separately to the NGM medium with Escherichia coli OP50, and place them in an incubator at 20 °C for 3 days;

[0071] (5) Suspend the nematodes cultured in (4) using M9 buffer (Na2HPO4: 6.0 g; KH2PO4: 3.0 g; NaCl: 5.0 g; MgSO4·7H2O: 0.25 g; dissolved in 1 L of distilled water, autoclave at high temperature and high pressure), and aspirate them into a culture tube. Add lysis solution (5 mol / L NaOH and 5% sodium hypochlorite solution by volume ratio) to each tube and lyse for 6 minutes;

[0072] (6) Then centrifuge the lysed liquid in (5) at 3500 r / min for 1 min, discard the supernatant, wash it 4 times repeatedly with M9 buffer, centrifuge again and discard the supernatant. Transfer the precipitate to the NGM medium and incubate overnight at 20 °C to obtain L1-stage nematode larvae;

[0073] (7) After rinsing and centrifuging once with M9 buffer, transfer the nematode larvae to an NGM plate containing Escherichia coli OP50 and incubate at 20 °C for 28 - 30 hours to obtain synchronized nematodes, that is, L4-stage nematodes;

[0074] (8) Randomly divide the obtained synchronized nematodes into three groups. The control group is fed with OP50, and the two experimental groups are fed with Lactobacillus mucosae A21224 and A21227 respectively, with 150 nematodes in each group. The day when L4-stage nematodes are picked is recorded as day 0. During the experiment, the nematodes are changed to NGM medium containing the corresponding strains every 2 days and the lifespan is counted until all nematodes die. Abnormally dead nematodes are not included in the statistics. Statistical analysis is performed using GraphPad Prism 9.

[0075] The experimental results are as Figure 2 shown. Lactobacillus mucosae A21224 can increase the lifespan of nematodes by 13.33%, and A21227 can increase the lifespan of nematodes by 20%.

[0076] Example 3 Antioxidant effects of Lactobacillus mucosae A21224 and A21227 in fermentation

[0077] This example provides a test on the antioxidant capacity of Lactobacillus mucosae A21224 and A21227 in fermentation. The specific experimental steps are as follows.

[0078] (1) Prepare synchronized Caenorhabditis elegans at the L4 stage, Escherichia coli OP50, Lactobacillus mucosae A21224 and A21227 in fermentation according to the experimental steps of Example 2;

[0079] (2) Randomly divide the synchronized nematodes into three groups. The control group is fed OP50, and the two experimental groups are fed Lactobacillus mucosae A21224 and A21227 in fermentation respectively. There are 30 nematodes in each group. The day when L4-stage nematodes are picked is recorded as day 0. On day 5, an antioxidant experiment is conducted on the nematodes. The experimental method is as follows: Prepare a 90 mM hydrogen peroxide solution in advance, take an appropriate amount and drop it on the NGM culture plate to make the liquid spread evenly on the surface of the culture medium. After the culture medium is dried, pick 30 nematodes from each group and place them in the above-treated petri dishes. Count once every 1 hour and record the number of dead nematodes.

[0080] The experimental results are as Figure 3 shown. Lactobacillus mucosae A21224 and A21227 in fermentation have good effects on the nematodes' resistance to oxidation. Compared with the control group, both Lactobacillus mucosae A21224 and A21227 in fermentation have increased the antioxidant capacity of Caenorhabditis elegans by 40%.

[0081] Example 4 Anti-thermal stress effects of Lactobacillus mucosae A21224 and A21227 in fermentation on nematodes

[0082] This example provides a test on the effects of Lactobacillus mucosae A21224 and A21227 in fermentation on the anti-thermal stress of Caenorhabditis elegans. The specific experimental steps are as follows.

[0083] (1) Prepare synchronized nematodes at the L4 stage, Escherichia coli OP50, Lactobacillus mucosae A21224 and A21227 in fermentation according to the experimental steps of Example 2;

[0084] (2) Randomly divide the synchronized nematodes into three groups. The control group is fed OP50, and the two experimental groups are fed Lactobacillus mucosae A21224 and A21227 in fermentation respectively. There are 30 nematodes in each group. The day when L4-stage nematodes are picked is recorded as day 0. On day 5, a thermal stress experiment is conducted on the nematodes. The experimental method is as follows: Put the control group and the experimental groups into a 37°C incubator at the same time and observe once every 1 hour. Record the number of dead nematodes.

[0085] The experimental results are as Figure 4As shown, Lactobacillus mucosae A21227 and A21224 have good effects on the anti-heat stress of nematodes. Under heat environmental stress, compared with the control group, feeding Lactobacillus mucosae A21227 and A21224 can both increase the lifespan of nematodes by 28.57%.

[0086] Example 5 Anti-aging effects of Lactobacillus mucosae A21224 and A21227

[0087] This example provides a test on the anti-aging ability of Lactobacillus mucosae A21224 and A21227. The specific experimental steps are as follows.

[0088] (1) Prepare L4 synchronized nematodes, Escherichia coli OP50, Lactobacillus mucosae A21224 and A21227 according to the experimental steps of Example 2;

[0089] (2) Randomly divide the synchronized nematodes into three groups. The control group is fed OP50, and the two experimental groups are fed Lactobacillus mucosae A21224 and A21227 respectively. Each group has 30 nematodes. The day when L4-stage nematodes are picked is recorded as day 0. On the 15th day of the experiment, head swing experiments and body bend experiments are carried out on nematodes in each group.

[0090] The head swing experiment is as follows: Count the frequency of head swings of nematodes within 30 s one by one in NGM plates containing M9 buffer. A head swing from one side to the other and back to the original position is counted as one swing. The body bend experiment is as follows: Count the frequency of body bends of nematodes within 30 s on blank NGM plates. A head swing from one side to the other and back to the original position is counted as one swing. Subsequently, statistical analysis is carried out using GraphPad Prism 9, and t-tests are used for analysis. P < 0.05 indicates a significant difference, and P < 0.01 indicates a highly significant difference.

[0091] The experimental results are as Figure 5 and Figure 6 shown. On the 15th day, both A21224 and A21227 have obvious effects of improving the motor ability of nematodes in the head swing and body bend experiments. Compared with the control group, the improvement effects of A21224 and A21227 in the head swing experiment are 64.51% and 106.48% respectively ( Figure 5 ), and the improvement effects in the body bend experiment are 270% and 527% respectively ( Figure 6 ). This example proves that Lactobacillus mucosae A21224 and A21227 have significant anti-aging effects.

[0092] Example 7 Artificial gastrointestinal juice tolerance experiment of Lactobacillus mucosae A21224

[0093] This example provides an in vitro experiment on the resistance of Lactobacillus mucosae A21224 to artificial gastrointestinal fluids. The specific experimental steps are as follows.

[0094] (1) Take the fermented Lactobacillus mucosae A21224 obtained in Example 1 for overnight culture activation, and inoculate the bacterial solution into artificial gastric juice (Regen Biotech) with a pH of 3 at an inoculation amount of 10% by volume.

[0095] (2) Take 100 μL of the solution at 0 h, 1 h, and 3 h respectively and spread it on an MRS plate, and perform single colony counting after anaerobic culture at 37 °C for 48 h.

[0096] (3) Use the bacterial solution treated with gastric juice for 3 h as the initial bacterial solution concentration for the intestinal juice experiment. Inoculate the bacterial solution into artificial intestinal juice (Regen Biotech) with a pH of 8, and take 100 μL of the solution at 2 h, 4 h, 6 h, and 8 h respectively and spread it on an MRS plate, and perform single colony counting after anaerobic culture at 37 °C for 48 h. Calculate the survival rate of Lactobacillus mucosae A21224 in artificial gastrointestinal fluids with the 0 h of gastric juice as the initial concentration according to the single colony count of the above experiment.

[0097] The experimental results are shown in Table 2 below. The survival rate of Lactobacillus mucosae A21224 in artificial gastric juice for 3 h is 129.13%, the survival rate in intestinal juice for 6 h is 27.18%, and the survival rate in intestinal juice for 8 h is 6.31%. This indicates that Lactobacillus mucosae A21224 has a certain colonization ability in the gastrointestinal tract without a cryoprotectant.

[0098] Table 2 Resistance of Lactobacillus mucosae A21114 to artificial gastrointestinal fluids

[0099]

[0100] Example 8 Resistance of Lactobacillus mucosae A21224 and A21227 to bile salts

[0101] This example provides an in vitro experiment on the resistance of Lactobacillus mucosae A21224 and A21227 to bile salts. The specific experimental steps are as follows.

[0102] Take the Lactobacillus mucosae A21227 and the fermented Lactobacillus mucosae A21224 obtained in Example 1 for overnight culture activation, and inoculate the bacterial solutions into MRS liquid medium with 0.1 g / L bile salts at an inoculation amount of 10% by volume. Take 100 μL of the solution at 0 h, 1 h, 2 h, 3 h, and 4 h respectively and spread it on an MRS plate, and perform single colony counting after anaerobic culture at 37 °C for 48 h.

[0103] The experimental results are shown in Table 3 below. The survival rates of Lactobacillus mucosae A21224 in bile salts at 1 h, 2 h, 3 h, and 4 h were 64.87%, 43.04%, 32.59%, and 40.19% respectively. The survival rates of Lactobacillus mucosae A21227 in bile salts at 1 h, 2 h, 3 h, and 4 h were 149.25%, 143.28%, 108.96%, and 208.96% respectively, indicating that A21224 and A21227 had good survival ability in 0.1 g / L bile salts, and A21227 could even proliferate in bile salts.

[0104] Table 3 Bile salt tolerance effects of Lactobacillus mucosae A21224 and A21227

[0105]

[0106] Example 9 Hemolysis experiment of Lactobacillus mucosae A21224 and A21227

[0107] This example provides a hemolysis test for Lactobacillus mucosae A21224 and A21227. The specific experimental steps are as follows.

[0108] The Lactobacillus mucosae A21227 and the Lactobacillus mucosae A21224 obtained in Example 1 were activated and cultured on a Columbia agar plate containing 5% sheep blood, and then placed in an anaerobic workstation at 37 °C for 48 h to observe whether there was a hemolytic zone around the colonies.

[0109] The experimental results are as Figure 7 and Figure 8 shown. There was no hemolytic zone around the colonies of A21224 ( Figure 7 ), and there was also no hemolytic zone around the colonies of A21227 ( Figure 8 ). Therefore, it was shown that both Lactobacillus mucosae A21224 and A21227 had high safety.

[0110] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A fermented Lactobacillus mucosae, characterized in that, The fermented Limosilactobacillus mucosae is Limosilactobacillus fermentum A21224, taxonomically named Limosilactobacillus fermentum, and was deposited at the Guangdong Provincial Culture Collection Center of Microorganisms on November 11, 2022, with the deposit number GDMCC No: 62958.

2. The culture of the fermented Limosilactobacillus mucosae according to claim 1.

3. The culture of Lactobacillus mucosae according to claim 2, characterized in that, The culture includes metabolites of Limosilactobacillus fermentum.

4. A bacterial agent, characterized in that, The microbial agent contains the fermented Limosilactobacillus mucosae according to claim 1 and / or the culture according to claim 2 or 3.

5. The microbial agent according to claim 4, wherein, The microbial agent is a solid microbial agent or a liquid microbial agent.

6. Use of any one or more of Limosilactobacillus fermentum, the culture of Limosilactobacillus fermentum, and the microbial agent containing Limosilactobacillus fermentum in at least one of the following (I) to (III); (I) Preparing a product for prolonging lifespan; (II) Preparing an anti-aging product; (III) Preparing an antioxidant product; The fermented Limosilactobacillus mucosae is Limosilactobacillus fermentum A21224 according to claim 1 and / or Limosilactobacillus fermentum A21227; The culture of the fermented Limosilactobacillus mucosae includes metabolites of Limosilactobacillus fermentum.

7. A product containing any one or several of the fermented Lactobacillus mucosae described in claim 1, the culture of the fermented Lactobacillus mucosae described in claim 2 or 3, and the bacterial agent described in claim 4 or 5, characterized in that The product includes food, food additives, feed, feed additives, drugs, and cosmetics.

8. The product according to claim 7, characterized in that, The product further contains excipients; the excipients include food additives, pharmaceutically acceptable adjuvants, or cosmetic excipients.

9. The product according to claim 7, characterized in that, The form of Limosilactobacillus fermentum in the product includes freeze-dried powder, bacterial liquid, and granular inoculum.

10. The product according to claim 7, characterized in that, The dosage form of the drug includes powder, granule, capsule, tablet, pill, or oral liquid.

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