Efficient breeding method for light-matrix bud-stock seedlings of xanthoceras sorbifolia bunge

Abstract

The invention belongs to the technical field of fruit tree seedling raising, and discloses an efficient breeding method for light-matrix bud stock seedlings of xanthoceras sorbifolia bunge. Comprising the steps of stock seedling cultivation, scion selection, collection and treatment, grafting and transplanting, post-grafting management and grafted seedling outplanting. The method is easy and convenient to operate, grafting and seedling growing are conducted in the same year, the grafted seedling cultivation period is shortened to one year, the seedling growing cost is reduced, and popularization is easy.

Description

Technical Field

[0001] The present invention belongs to the technical field of fruit tree seedling cultivation, and particularly relates to an efficient breeding method for light matrix budstock seedlings of Xanthoceras sorbifolia Bunge. Background Art

[0002] Xanthoceras sorbifolia Bunge is a unique woody oil tree species in China, mainly distributed in Gansu, Shaanxi, Inner Mongolia, Liaoning, Shandong and other places. Xanthoceras sorbifolia Bunge has wide application values in aspects such as food, medicine, and afforestation of barren mountains. Especially, the oil content of its seeds is between 30% and 50%, and the oil content of the kernel is as high as 65%. It can not only be used to make edible oil, but also be used as a raw material for preparing biodiesel. At present, the bottleneck in the development of the Xanthoceras sorbifolia Bunge industry is low yield and low efficiency. The main reason for the low yield is the low utilization rate of improved varieties and the lack of efficient and reliable breeding methods.

[0003] Currently, due to the high cost of tissue culture, its large-scale use is often restricted in practical applications. Seedling propagation and grafting are the most common propagation methods for Xanthoceras sorbifolia Bunge. At present, most newly planted Xanthoceras sorbifolia Bunge forests are propagated from seedling. The genetic material of seedling will undergo recombination and variation, and the excellent characteristics of the mother tree cannot be completely maintained. There are certain risks in the large-scale promotion of seedling. At present, most of the selected excellent varieties in various places in China are propagated by grafting. By cultivating grafted seedlings as a stable and efficient technology for fixing excellent traits and large-scale breeding of Xanthoceras sorbifolia Bunge, the demand for high-quality asexually propagated seedlings for large-scale industrial construction of Xanthoceras sorbifolia Bunge can be met.

[0004] Currently, the nursery period of grafted seedlings is generally 2 - 3 years, that is, the rootstock seedlings are cultivated in the first year, and after grafting in the second and third years, they grow to the standard of outplanting seedlings. For example, in the patent "Xanthoceras sorbifolia Bunge Seedling Cultivation Technology (Patent No.: 200810150333.7)", with large-piece bud grafting with xylem, the rootstock seedlings are bred in the first year. After one-year growth, in late April of the second year, seedlings with a diameter of more than 1 cm are selected as rootstocks, and high-yield, robust and developed branches are selected as scions for grafting. After grafting, wait for the buds to grow and develop, and finally reach the outplanting conditions. The nursery period is about 2.5 years, and the nursery period is relatively long; in the method of "A Method for Breeding Grafted Container Seedlings of Xanthoceras sorbifolia Bunge Seedlings (Patent No.: CN201610250815.4)", grafting is carried out in spring and summer. The seedling has not reached the required thickness for grafting and cannot match the scion, resulting in strict scion selection and low grafting survival rate. The ground diameter of the seedlings of this method is less than 6 mm when the growth stops in autumn, the growth period is short and the growth is weak; if grafting is carried out in autumn, the grafted seedlings need to grow into seedlings in the second year to meet the outplanting requirements.

[0005] Due to reasons such as poor matching between rootstock and scion, difficult wound healing, and low germination rate of new buds, the budstock seedling technology has not been used in the actual production of Xanthoceras sorbifolia Bunge seedling cultivation. Summary of the Invention

[0006] In order to overcome the deficiencies of the prior art, the present invention provides an efficient breeding method for light substrate budstock seedlings of Xanthoceras sorbifolium, and for the first time applies the budseedling grafting technology to the seedling raising practice of Xanthoceras sorbifolium, and optimizes the cultivation of rootstock seedlings, the selection, collection and treatment of scions, grafting and transplanting, post-grafting management, and the outplanting of grafted seedlings according to the characteristics of Xanthoceras sorbifolium.

[0007] The above object of the present invention is achieved by the following technical solutions: An efficient breeding method for light substrate budstock seedlings of Xanthoceras sorbifolium, the steps include:

[0008] 1. Cultivation of rootstock seedlings: Harvest the fruits of Xanthoceras sorbifolium as seeds for cultivating rootstock seedlings, air-dry and store them, select the seeds of Xanthoceras sorbifolium as the seed stock for cultivating bud seedlings, disinfect the seeds and the sand bed, spread the treated seeds on the sand bed for moisture and heat preservation operations, and graft when the rootstock seedlings of the seeds grow to a certain length.

[0009] 2. Selection, collection and treatment of scions: Select Xanthoceras sorbifolium trees as scion mother trees, collect the scion branches of the scion mother trees, and cut the scions prepared for grafting with the seedling buds in step 1.

[0010] 3. Grafting and transplanting: Dig out the rootstock seedlings with the bud seedlings growing to a certain length in step 1, wash the sand grains, cut the seedling rootstock and the scion, match the thickness of the rootstock and the scion, insert the scion into the rootstock, and then bind and graft into grafted seedlings, and then perform transplanting operations on the grafted seedlings.

[0011] 4. Post-grafting management: Build a plastic arch shed for moisture preservation after transplanting the grafted seedlings, remove the plastic film after a certain period of time, cut off the sprouts on the rootstock, remove weeds and dead plants, and spray nutrient solution every once in a while.

[0012] 5. Outplanting of grafted seedlings: Outplant when the grafted seedlings grow to a certain length or more in ground diameter, or transplant with containers in the spring of the second year.

[0013] Further, the specific steps of step 1 are as follows:

[0014] 1.1. Seed harvesting and storage: Harvest when the fruits of Xanthoceras sorbifolium are completely mature in early August, and select the high-quality seeds of the current year with plump grains, uniform size and complete maturity as the seeds for cultivating rootstock seedlings. After harvesting, air-dry naturally and store in sand at 4°C.

[0015] 1.2. Seed germination acceleration and sowing: In the early ten days of April of the next year, select seeds with large and plump fruit grains and no pest or disease damage as the seedlings for cultivating budded rootstocks. Treat the seeds and the sand bed with 0.2% thiophanate-methyl carbendazim for disinfection. After disinfection, spread the seeds flat on the sand bed, cover the seeds with 8 cm thick river sand, cover the sand layer with a plastic film, with a humidity of 30% and a temperature of 20°C, to keep moisture and warmth. Monitor the seed germination process. When the bud seedlings grow to 5 cm after about 1 month, grafting can be carried out. If the scion does not grow to the thickness matching the bud seedlings due to climate reasons and the bud seedlings have emerged from the soil, then cover the bud seedlings with wet sand to extend the germination period, thicken the bud rootstock, and avoid lignification of the bud seedlings after they emerge from the soil.

[0016] Further, the specific content of step 2 is as follows: Select excellent clones or improved varieties of Xanthoceras sorbifolium Bunge in the suitable growth area as the scion mother trees. In the middle ten days of May, collect the current-year branches on the mother trees that are more than 10 cm long, with plump bud development and no pests or diseases, and make variety marks. Cut the scions on the day of grafting, make a cut at 2 cm above and below the axillary buds at both ends, remove most of the leaves, and only retain 1 - 2 leaves at the bottom of the petioles.

[0017] Further, the specific content of step 3 is as follows:

[0018] 3.1. Rootstock lifting

[0019] When lifting the rootstock from the seedbed, first dig out the rootstock seedlings, clean up the river sand at the radicle part of the seedlings, wash the sand grains with a 0.1% carbendazim solution, retain the seeds on the rootstock seedlings and avoid breaking the roots;

[0020] 3.2. Grafting

[0021] Cut the rootstock: Cut off the radicle 7 cm below the cotyledon petiole and cut off the plumule 1.5 - 3 cm above the cotyledon petiole, with the cross-section perpendicular to the plumule; When opening the rootstock, make a longitudinal cut in the middle of the cotyledon, and the depth of opening the rootstock is 0.8 - 1.2 cm, and the depth should be equivalent to the length of the scion cut surface;

[0022] Cut the scion: The cutting position of the scion is 1 - 1.5 cm from the base of the bud, the length of the cut surface is 0.5 - 0.8 cm, the cut-off head is 1 cm above the bud tip, and the cut surface should be flat, regular and smooth; Graft immediately after opening the rootstock to prevent browning of the rootstock and scion;

[0023] The thickness of the rootstock and scion should match. Insert the scion into the rootstock and quickly tie it with an aluminum foil sheet, with appropriate tightness during tying; During the grafting process, it is necessary to keep the scion branches moist, the cut scions moist, the rootstock moist, the grafted products moist and keep them moist with a wet towel during transportation;

[0024] 3.3. Transplanting

[0025] Before transplanting the grafted seedlings, prepare light substrate mesh bag containers and fill them with 55% peat soil, 25% perlite and 20% vermiculite; after watering, cover with 3 cm of river sand to ensure that the humidity of the seedbed is about 30%; the planting depth is 5-6 cm, ensure that the cotyledons are buried in the sand, and at the same time ensure that the grafting joint is exposed outside the soil layer. After planting, water thoroughly with 0.2% thiophanate methyl carbendazim.

[0026] Further, step 4 is specifically as follows:

[0027] After planting, it is necessary to build a plastic arch shed for moisturizing. The height of the shed is 50-60 cm, and the plastic film around the arch shed is buried in the soil to ensure the sealing of the arch shed for heat and moisture preservation (the temperature is preferably 23 °C, the maximum does not exceed 30 °C, and the humidity is 90%). The plastic film can be removed after one month; after removing the plastic film of the arch shed, remove the buds on the rootstock, remove weeds and dead plants. The nutrient solution can be sprayed once a month, and the spraying amount is 10 ml / seedling. The concentration of the nutrient solution (m / m%): urea, 0.2%; ammonium phosphate, 0.6%; potassium dihydrogen phosphate 1%.

[0028] Further, in step 5, the grafted seedlings are taken out of the nursery when the ground diameter grows to more than 0.7 cm.

[0029] The beneficial effects of the present invention compared with the prior art are: the method is easy to operate, the grafted seedlings can become seedlings in the same year, the cultivation period of the grafted seedlings is shortened to 1 year, the seedling raising cost is reduced, and it is easy to promote. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The present invention will be further described below in conjunction with the drawings and specific embodiments

[0031] Figure 1 is a schematic diagram of the storage of Xanthoceras sorbifolium seeds in the present invention;

[0032] Figure 2 is a schematic diagram of sowing and germination acceleration of Xanthoceras sorbifolium in the present invention;

[0033] Figure 3 is a schematic diagram of the rootstock of Xanthoceras sorbifolium seedlings in the present invention;

[0034] Figure 4 is a schematic diagram of the grafting of Xanthoceras sorbifolium bud seedlings in the present invention;

[0035] Figure 5 is a schematic diagram of the transplantation after the grafting of Xanthoceras sorbifolium bud seedlings in the present invention;

[0036] Figure 6 is a schematic diagram of the germination and growth of Xanthoceras sorbifolium grafted seedlings in the arch shed in the present invention. SPECIFIC EMBODIMENTS

[0037] The present invention will be described in detail below through specific embodiments, but the protection scope of the present invention is not limited. Unless otherwise specified, the experimental methods used in the present invention are all conventional methods, and the experimental equipment, materials, reagents, etc. used can be obtained from commercial channels.

[0038] Example 1

[0039] 1. On July 30, 2023, harvesting was carried out. Select high-quality seeds of the current year with plump grains, uniform size, and complete maturity as the seeds for cultivating rootstock seedlings. After harvesting, let them dry naturally and store them at 4°C. The storage location should be kept dry and ventilated.

[0040] 2. On April 2, 2024, after germination, sow Xanthoceras sorbifolium seeds in a sand bed.

[0041] 3. In mid-May, collect current-year shoots on the mother tree that are more than 10 cm long, have plump bud development, and are free of pests and diseases, and make variety markings. Cut the scions on the day of grafting, make a cut at 2 cm above and below the axillary bud at both ends, remove most of the leaves, and only retain 1-2 leaves at the bottom of the petiole.

[0042] 4. Lift the rootstock seedlings

[0043] When lifting the rootstock from the seedbed, first dig out the rootstock seedlings, clean up the river sand at the radicle part of the seedlings, wash the sand grains with a carbendazim solution with a concentration of 0.1%, retain the seeds on the rootstock seedlings and avoid breaking the roots.

[0044] 5. Grafting

[0045] Trim the rootstock seedlings. Cut off the radicle 7 cm below the cotyledon petiole and cut off the plumule 1.5 - 3 cm above the cotyledon petiole. The cut surface is perpendicular to the plumule. When opening the rootstock, make a longitudinal cut in the middle of the cotyledon, and the opening depth is 0.8 cm. The depth should be equivalent to the length of the scion cut surface. The cut surface should be flat, regular, and smooth. The thickness of the rootstock and the scion should match. Insert the scion into the rootstock and quickly tie it with an aluminum foil strip. When tying, the tightness should be appropriate.

[0046] 6. Before transplanting the grafted seedlings, water the seedbed in advance to ensure that the humidity of the seedbed is about 30%. The seedling bags are pre-filled with river sand with a humidity of 30%. The planting depth is 5 - 6 cm, ensuring that the cotyledons are buried in the sand, and at the same time ensuring that the grafting interface is exposed outside the soil. After planting, water thoroughly with 0.2% thiophanate-methyl carbendazim.

[0047] 7. Build an arch shed: After planting, it is necessary to build a plastic arch shed for moisture preservation. The height of the shed is 50 - 60 cm. The plastic film around the arch shed is buried in the soil to ensure that the arch shed is sealed for heat and moisture preservation (the temperature is preferably 23°C, the maximum does not exceed 30°C, and the humidity is 90%). The plastic film can be removed after one month.

[0048] 8. After removing the plastic film of the arch shed, the budding on the rootstock can be removed, and weeds and dead plants can be removed. The nutrient solution can be sprayed once a month, with a spraying amount of 10 ml / seedling. The concentration of the nutrient solution (m / m%): urea, 0.2%; ammonium phosphate, 0.6%; potassium dihydrogen phosphate 1%.

[0049] 9. When the diameter of the grafted seedling at the ground level is greater than 0.7 cm, it can be taken out of the nursery, or transplanted with a pot in the following spring.

[0050] Comparative Example 1

[0051] Traditional grafting method

[0052] (1) Technical solution: Refer to the patent "Xanthoceras sorbifolium seedling raising technology (Patent No.: 200810150333.7)", and adopt the large bud piece budding method with xylem.

[0053] (2) Steps: Rootstock cultivation: Cultivate rootstock seedlings by sowing in the first year, and select rootstocks with a ground diameter ≥ 1 cm in the following year. Scion selection: Select 1-year-old healthy branches of the mother tree and retain 2-3 buds. Grafting: Cut grafting in late April, and bind and moisturize after grafting. Management: Outdoor maintenance, conventional water and fertilizer management.

[0054] (3) Experimental results:

[0055] Seedling raising cycle: 1 year for rootstock cultivation + 1.5 years for growth after grafting, with a total cycle of 2.5 years.

[0056] Survival rate: The grafting survival rate is 65%-70%.

[0057] Quality of seedlings: The average ground diameter at the time of outplanting is 0.6 cm, and the seedling height is 30-40 cm.

[0058] (4) Comparative analysis: Through the bud seedling rootstock grafting technology of the present invention, the seedling raising cycle is shortened to 1 year, the survival rate is increased to over 90%, and the ground diameter of the outplanted seedlings ≥ 0.7 cm, which is significantly better than the traditional method.

[0059] Comparative Example 2

[0060] Effect of different grafting times on survival rate (control within the scheme)

[0061] (1) Technical solution: Experimental group 1: Grafting in late April according to the existing technology (the semi-lignification degree of the scion is insufficient); Experimental group 2: Grafting in mid-May according to the present invention (the scion is semi-lignified and the bud seedlings grow to 10 cm); Experimental group 3: Grafting in early May according to the existing technology.

[0062] (2) Experimental conditions: The rootstock cultivation, scion treatment, and management methods are all the same as those of the present invention.

[0063] (3) Experimental results:

[0064]

[0065]

[0066] Comparative Example 3

[0067] Effect of Different Moisture Retention Conditions on the Growth of Grafted Seedlings (Control within the Scheme)

[0068] (1) Technical Solution: Control Group: Only covered with ordinary plastic film after transplanting (not sealed, humidity maintained at 60%-70%, temperature 23°C).

[0069] Experimental Group: Built a sealed arch shed according to the present invention (humidity 90%, temperature 23°C).

[0070] (2) Experimental Results:

[0071]

[0072] Moisture retention in the sealed arch shed can accelerate the healing of the interface, reduce water loss, and improve the germination rate of new buds.

[0073] Comparative Example 4

[0074] Comparison with the Prior Art of Patent CN201610250815.4

[0075] (1) Technical Solution: Refer to "A Method for Breeding Grafted Container Seedlings of Xanthoceras sorbifolium Bunge", grafting in autumn.

[0076] (2) Implementation Results: Seedling Raising Period: It needs to grow in the second year after autumn grafting, with a total period of 1.5 years. Survival Rate: 75%-80%.

[0077] Seedling Quality: Ground diameter ≤ 0.6 cm, weak root system.

[0078] (3) Comparative Analysis: By optimizing the grafting time and supporting management in spring, the present invention can achieve seedlings in the same year (ground diameter ≥ 0.7 cm), and the root system is developed, which is significantly better than the autumn grafting scheme.

[0079] Comparative Example 5

[0080] Effect of Grafting Depth on the Survival Rate of Grafted Seedlings (Control within the Scheme)

[0081] (1) Technical Solution: Experimental Group 1: Grafting depth 0.6 cm; Experimental Group 2: 0.8 cm (grafting depth according to the prior art); Experimental Group 3: Grafting depth 1 cm.

[0082] (2) Experimental Results:

[0083]

[0084] As can be seen from the above comparative examples, the budstock grafting method of the present invention shortens the seedling raising period to 1 year by optimizing the grafting time, moisture retention conditions, scion cutting depth and the matching degree between the stock and scion, and at the same time increases the survival rate to over 90%. The quality of the seedlings is significantly better than any existing technology, and it has remarkable innovation and practicability.

[0085] The above-described embodiments are only the preferred embodiments of the present invention, and not all the feasible embodiments of the present invention. For those of ordinary skill in the art, any obvious changes made without departing from the principles and spirit of the present invention should be considered to be included within the scope of protection of the claims of the present invention.

Claims

1. A method for efficient propagation of Xanthoceras sorbifolium light substrate budded seedlings, characterized in that the steps Including: S1. Cultivation of rootstock seedlings: Harvest the fruits of Xanthoceras sorbifolium as the seeds for cultivating rootstock seedlings, air-dry and store them. Select the seeds of Xanthoceras sorbifolium as the seed varieties for cultivating bud seedlings on rootstock. Disinfect the seeds and the sand bed. Spread the treated seeds evenly on the sand bed for moisture and temperature preservation operations. After the rootstock seedlings grow to a certain length, graft them. S2. Selection, collection and treatment of scions: Select Xanthoceras sorbifolium trees as the scion mother trees, collect the scion branches of the scion mother trees, and cut the scions prepared for grafting with the seedling buds on rootstock in step S1. S3. Grafting and transplanting: Dig out the rootstock seedlings with the bud seedlings growing to a certain length in step S1, wash off the sand grains, cut the rootstock on the seedling and the scion, match the thickness of the rootstock and the scion, insert the scion into the rootstock, and then bind and graft them into grafted seedlings, and then perform transplanting operations on the grafted seedlings. S4. Management after grafting: Build a plastic arch shed for moisture preservation after transplanting the grafted seedlings. Remove the plastic film after a certain period of time, cut off the sprouts on the rootstock, remove weeds and dead plants, and spray nutrient solution every once in a while. S5. Outplanting of grafted seedlings: Outplant when the ground diameter of the grafted seedlings grows to a certain length or more, or transplant them with containers in the spring of the second year.

2. The efficient propagation method of Xanthoceras sorbifolium light substrate budded seedlings according to claim 1, characterized in that, The specific steps of step S1 are as follows: S1.

1. Seed harvesting and storage: Harvest in early August when the fruits of Xanthoceras sorbifolium are completely mature. Select the high-quality seeds of the current year with plump grains, uniform size and complete maturity as the seeds for cultivating rootstock seedlings. After harvesting, air-dry them naturally and store them in sand at 4°C. S1.

2. Seed germination promotion and sowing: Select the seeds with large and plump grains and no pest and disease damage as the seed varieties for cultivating bud seedlings on rootstock in early April of the following year; Treat and disinfect the seeds and the sand bed with 0.2% thiophanate-methyl carbendazim. After disinfection, spread the seeds evenly on the sand bed, cover the seeds with 8 cm thick river sand, cover the sand layer with a plastic film, with a humidity of 30% and a temperature of 20°C for moisture and temperature preservation. Monitor the seed germination process. When the bud seedlings grow to 5 cm after about 1 month, graft them; If the scion does not grow to the thickness matching the bud seedlings due to climate reasons and the bud seedlings have emerged from the soil, then cover the bud seedlings with wet sand to extend the germination period and thicken the bud rootstock to avoid lignification of the bud seedlings after they emerge from the soil.

3. The efficient propagation method of Xanthoceras sorbifolium light substrate bud rootstock seedlings according to claim 1, characterized in that, The specific steps of step S2 are as follows: Select excellent clones or improved varieties of Xanthoceras sorbifolium in the suitable growing area as the scion mother trees. In mid-May, collect the current-year branches on the mother trees with a length of more than 10 cm, plump bud development and no pests and diseases, and make variety marks; Cut the scions on the day of grafting, make a cut at 2 cm above and below the axillary buds at both ends, and remove most of the leaves, only retaining 1-2 leaves at the bottom of the petiole.

4. The efficient propagation method of Xanthoceras sorbifolium light substrate budstock seedlings according to claim 1, characterized in that, The specific steps of step S3 are as follows: S3.

1. Uprooting of rootstock seedlings When uprooting the rootstock from the seedbed, first dig out the rootstock seedlings, clean up the river sand at the radicle part of the seedlings, wash off the sand grains with a 0.1% carbendazim solution, retain the seeds on the rootstock seedlings and avoid breaking the roots. S3.

2. Grafting Cut the rootstock on the seedling. Cut off the radicle 7 cm below the cotyledon petiole and cut off the plumule 1.5-3 cm above the cotyledon petiole. The cut surface is perpendicular to the plumule; When opening the rootstock, make a longitudinal cut in the middle of the cotyledon, and the depth of opening the rootstock is 0.8-1.2 cm, and the depth should be equivalent to the length of the cut surface of the scion. Trim the scion. The cutting position of the scion is 1 - 1.5 cm away from the base of the bud, the length of the cut surface is 0.5 - 0.8 cm, the broken end is 1 cm above the bud tip, and the cut surface should be flat, regular, smooth; graft immediately after preparing the rootstock to prevent browning of the rootstock and scion; The thickness of the rootstock and scion should match. Insert the scion into the rootstock and quickly tie it with an aluminum foil strip, with appropriate tightness during tying; during the grafting process, ensure moisture retention for the scion branch, the prepared scion, the rootstock, the grafted finished product, and use a wet towel for moisture retention during transportation; S3.

3. Transplanting Before transplanting the grafted seedlings, prepare light substrate net bag containers and fill them with 55% peat soil, 25% perlite, and 20% vermiculite; cover with 3 cm of river sand after watering to ensure the humidity of the seedbed is about 30%; the planting depth is 5 - 6 cm, ensure that the cotyledons are buried in the sand, and at the same time ensure that the grafting joint is exposed outside the soil layer. After planting, water thoroughly with 0.2% thiophanate methyl carbendazim.

5. The efficient propagation method of Xanthoceras sorbifolium light substrate bud seedlings according to claim 1, characterized in that, The specific step S4 is as follows: After planting, a plastic arch shed needs to be built for moisture retention, the height of the shed is 50 - 60 cm, and the plastic film around the arch shed is buried in the soil to ensure the arch shed is sealed for heat and moisture preservation; remove the plastic film after one month; after removing the plastic film of the arch shed, remove the sprouts on the rootstock, remove weeds and dead plants. Spray the nutrient solution once a month, the spraying amount is 10 ml / seedling, and the nutrient solution concentration is in m / m%: urea, 0.2%; ammonium phosphate, 0.6%; potassium dihydrogen phosphate 1%.

6. The efficient propagation method of Xanthoceras sorbifolium light substrate budded seedlings according to claim 5, characterized in that, The operation parameters for heat and moisture preservation are: temperature 23°C - 30°C, humidity 90%.

7. The efficient propagation method of Xanthoceras sorbifolium light substrate bud seedlings according to claim 1, characterized in that In step S5, the grafted seedlings are lifted out of the nursery when the ground diameter grows to more than 0.7 cm.

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