Method for preparing feed additive for relieving constipation of sows by using traditional Chinese medicine formula

Abstract

The invention discloses a method for preparing a feed additive for relieving constipation of sows by using a traditional Chinese medicine formula, which comprises the following steps: preparing enzymolysis primary wet powder, preparing an enzymolysis powder fermentation material, preparing a polysaccharide compound iron selenium liquid, preparing a polysaccharide compound iron selenium fermentation material, preparing an acidified compound iron selenium fermentation material and preparing the feed additive. Through a new traditional Chinese medicine formula and acidification fermentation treatment of iron, selenium and polysaccharide, the complexation of iron, selenium and polysaccharide is increased, and the produced feed additive can improve the body vitality of sows, promote intestinal health, increase intestinal peristalsis and greatly relieve constipation of sows.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additive preparation, and specifically relates to a method for preparing a feed additive for relieving sows' constipation using a traditional Chinese medicine formula. Background Art

[0003] Pork pigs are the largest source of edible meat globally. Pork has a delicious taste and is suitable for human consumption, being popular among consumers. The output efficiency of sows is an important part of the breeding efficiency. The intestinal health care of sows is an important link to improve the efficiency of sows, and one trend of intestinal health care is being green, efficient, and safe.

[0004] During the feeding process of pigs, the constipation problem of sows has always been a stubborn problem. The constipation of sows not only affects the nutrient absorption of sows, but also affects the number of litters, the average litter weight, the number of healthy piglets, etc. Therefore, relieving the constipation of sows is a key link to improve the breeding efficiency of sows. Using natural plants to produce feed functional additives has the characteristics of multiple action targets, significant effects, no toxic side effects, being safe and green, etc. The plant raw material additives can play a good role in the intestinal health care of pigs.

[0005] Chinese Patent Document CN202211627180.7 discloses a traditional Chinese medicine composition for preventing and treating constipation in pregnant sows and its preparation method. The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 25 - 30 parts of Scrophulariae Radix, 21 - 25 parts of Ophiopogonis Radix, 20 - 25 parts of Rehmanniae Radix, 10 - 15 parts of Artemisia sphaerocephala Krasch., 6 - 8 parts of Wengbo, 6 - 8 parts of Perillae Caulis. Although the prepared traditional Chinese medicine composition can improve the symptoms of sows' constipation to a certain extent, the Chinese herbal medicine components are only simply extracted, and the medicinal effects of traditional Chinese medicine are not fully utilized, and the prevention and treatment effects still need to be further improved. Summary of the Invention

[0006] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a method for preparing a feed additive for relieving sows' constipation using a traditional Chinese medicine formula.

[0007] To achieve the above purpose, the present invention adopts the following technical solutions:

[0008] A method for preparing a feed additive for relieving sows' constipation using a traditional Chinese medicine formula, comprising the following steps:

[0009] S1. Preparation of enzymatically hydrolyzed primary wet powder: Add wax gourd powder, Ficus hirta Vahl., Stevia rebaudiana Bertoni, and Luffa cylindrica (L.) Roem. extract into water, mix evenly to obtain primary wet powder, and then add hemicellulase, cellulase, and pectinase to the primary wet powder for enzymatic hydrolysis reaction to obtain enzymatically hydrolyzed primary wet powder;

[0010] S2. Preparation of enzymatically hydrolyzed powder fermented material: Add the Saccharomyces cerevisiae liquid, Clostridium butyricum liquid, and Lactobacillus plantarum liquid to the enzymatically hydrolyzed primary wet powder obtained in step S1, stir evenly, seal and ferment to obtain the enzymatically hydrolyzed powder fermented material;

[0011] S3. Preparation of polysaccharide complex iron and selenium liquid: Mix glutinous sorghum, Polygonum chinense, Phellinus igniarius, and fingered citron evenly to obtain a mixed powder, then add ferrous fumarate, nano-selenium, cellulase, hemicellulase, and water thereto, mix evenly, and keep warm at 45 - 60 °C for 6 - 10 h to obtain the polysaccharide complex iron and selenium liquid;

[0012] S4. Preparation of polysaccharide complex iron and selenium fermented material: Add glucose and Clostridium butyricum liquid to the polysaccharide complex iron and selenium liquid, stir evenly, seal and ferment to obtain the polysaccharide complex iron and selenium fermented material;

[0013] S5. Preparation of acidified complex iron and selenium fermented material: Add citric acid monohydrate and water to the polysaccharide complex iron and selenium fermented material, keep warm at 55 - 65 °C for 0.5 - 2 h, then add lactic acid powder, continue to keep warm for 2 - 4 h, and dry to obtain the acidified complex iron and selenium fermented material;

[0014] S6. Preparation of feed additive: Mix the enzymatically hydrolyzed powder fermented material, acidified complex iron and selenium fermented material, and water evenly, and ferment to obtain the feed additive.

[0015] Preferably, in step S1, the mass ratio of wax gourd powder, Ficus hirta, sweet leaf bacteria, loofah sponge extract, and water is 1 - 3:10 - 15:2 - 4:15 - 20:15 - 30.

[0016] Preferably, in step S1, based on the mass of the primary wet powder, the addition amount of hemicellulase is 5%, the addition amount of cellulase is 5%, and the addition amount of pectinase is 3%;

[0017] The temperature of the enzymatic hydrolysis reaction is 38 - 42 °C, and the time of the enzymatic hydrolysis reaction is 1 - 3 h.

[0018] Preferably, in step S2, based on the mass of the enzymatically hydrolyzed primary wet powder, the addition amount of Saccharomyces cerevisiae liquid is 1% (v / w), the addition amount of Clostridium butyricum liquid is 1% (v / w), and the addition amount of Lactobacillus plantarum liquid is 1% (v / w).

[0019] Preferably, in step S2, the temperature of the sealed fermentation is 30 - 33 °C, and the time of the sealed fermentation is 72 - 144 h.

[0020] Preferably, in step S3, the mass ratio of glutinous sorghum, Polygonum chinense, Phellinus igniarius, and fingered citron is 1 - 3:18 - 25:12 - 16:2 - 5; the mass ratio of ferrous fumarate, nano-selenium, cellulase, hemicellulase, water, and the mixed powder is 0.01:0.002:6:8:250:100.

[0021] Preferably, in step S4, based on the mass of the polysaccharide composite iron-selenium solution, the amount of glucose added is 1% (w / w), and the amount of Clostridium butyricum solution added is 1% (v / w);

[0022] The temperature of sealed fermentation is 30-33°C, and the time of sealed fermentation is 72-144h.

[0023] Preferably, in step S5, the mass ratio of citric acid monohydrate, water, polysaccharide composite iron-selenium fermentation material, and lactic acid powder is 8-15:200-300:100:15-20.

[0024] Preferably, in step S6, the mass ratio of the enzymatic hydrolysis powder fermentation material, the acidified composite iron-selenium fermentation material and water is 6-8:4-6:2-4;

[0025] The fermentation temperature is 30-33°C, and the sealed fermentation time is 24-48 hours.

[0026] The present invention also provides a feed additive prepared by the method.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The present invention increases the complexation of iron, selenium and polysaccharide through a new traditional Chinese medicine formula and acidification and fermentation of iron, selenium and polysaccharide. The produced feed additive can improve the vitality of sows, promote intestinal health, increase intestinal peristalsis and significantly relieve constipation of sows. DETAILED DESCRIPTION

[0029] The present invention is further described in detail below through specific preferred embodiments, but the present invention is not limited to the following embodiments.

[0030] It should be noted that, unless otherwise specified, all chemical reagents involved in the present invention were purchased through commercial channels.

[0031] Clostridium butyricum was purchased from Qingdao Weilan Biotechnology Co., Ltd.;

[0032] Saccharomyces cerevisiae was purchased from Qingdao Weilan Biotechnology Co., Ltd.

[0033] Lactobacillus plantarum was purchased from Qingdao Weilan Biotechnology Co., Ltd.;

[0034] Hemicellulase was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., 50,000 U / g;

[0035] Cellulase was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., 11000 U / g;

[0036] Pectinase (H type / viscosity reducing) was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., 25000 U / g;

[0037] Winter melon powder was purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0038] Prunus mume was purchased from Nanjing Tongrentang Pharmaceutical Co., Ltd.

[0039] Luffa extract was purchased from Xi'an Qiancao Biotechnology Co., Ltd.;

[0040] Sweet leaf mushrooms were purchased from Fengyushun Planting Professional Cooperative in Keyouqian Banner;

[0041] Glutinous sorghum was purchased from Xingyuan Grain and Oil Company in Luozhuang District;

[0042] Phytophthora pyrophora was purchased from Xi'an Qiancao Biotechnology Co., Ltd.;

[0043] Phellinus igniarius was purchased from Zhuoma Ama flagship store;

[0044] Buddha's hand was purchased from Wenjie Chinese Herbal Medicine Trading Company in Juancheng County, a strong supplier;

[0045] Peptone was purchased from Jinan Lanjue Trading Co., Ltd.

[0046] Glucose was purchased from Shandong Xujie Chemical Co., Ltd.;

[0047] Ferrous fumarate was purchased from Nanning Zeweier Feed Co., Ltd.;

[0048] Nano-selenium was purchased from Chengdu Chelation Biotechnology Co., Ltd.;

[0049] Citric acid monohydrate was purchased from Shanghai Mingjiong Biotechnology Co., Ltd.;

[0050] Lactic acid powder was purchased from Qingdao Huazhiyuan Food Additive Co., Ltd.

[0051] In each embodiment and comparative example of the present invention, the activation process of the bacterial solution is as follows:

[0052] Scrape six rings of Clostridium butyricum from the slant surface using an inoculating loop (0.3 mm in diameter), inoculate with 300 mL of activated culture medium, place in a 500 mL Erlenmeyer flask, and incubate at 33°C, 180 rpm, and culture for 20 h to obtain a Clostridium butyricum solution.

[0053] Scrape six rings of Saccharomyces cerevisiae from the slant with an inoculating loop (0.3 mm in diameter), inoculate with 300 mL of activation culture medium, place in a 500 mL Erlenmeyer flask, and incubate at 33°C, 180 rpm for 20 h to obtain a Saccharomyces cerevisiae culture solution.

[0054] For the Lactobacillus plantarum strain preserved on an inclined plane, scrape six loops of bacteria with an inoculation loop (loop diameter 0.3 mm), inoculate into 300 mL of activation medium, place in a 500 mL Erlenmeyer flask, culture at 33 °C with a rotation speed of 180 r / m, and culture the yeast for 22 h to obtain a Lactobacillus plantarum bacterial solution;

[0055] Composition of the medium: yeast extract 1% (w / w), peptone 1% (w / w), glucose 4% (w / w); after preparation, sterilize at 115 °C for 30 min and cool to room temperature to obtain.

[0056] Winter melon powder, Ficus simplicissima Lour., Luffa cylindrica (L.) Roem. extract, Phellinus igniarius, glutinous sorghum, Polygonum chinense L., and fingered citron: pulverize them separately with a pulverizer and pass through an 80-mesh sieve after pulverization.

[0057] Example 1

[0058] A method for preparing a feed additive for relieving sows' constipation using a traditional Chinese medicine formula, comprising the following steps:

[0059] S1. Preparation of enzymatically hydrolyzed primary wet powder: Add winter melon powder, Ficus simplicissima Lour., sweet leaf bacteria, and Luffa cylindrica (L.) Roem. extract to water, where the mass ratio of winter melon powder, Ficus simplicissima Lour., sweet leaf bacteria, Luffa cylindrica (L.) Roem. extract to water is 2:11:3:16:15, mix evenly to obtain primary wet powder. Then, based on the mass of the primary wet powder, add 5% hemicellulase, 5% cellulase, and 3% pectinase, and keep warm at 40 °C for 2.0 h to obtain enzymatically hydrolyzed primary wet powder;

[0060] S2. Preparation of enzymatically hydrolyzed powder fermented material: Add 1 mL of Saccharomyces cerevisiae bacterial solution, 1 mL of Clostridium butyricum bacterial solution, and 1 mL of Lactobacillus plantarum bacterial solution to 100 g of the enzymatically hydrolyzed primary wet powder obtained in step S1, stir evenly, place in a plastic bag, and ferment at 32 °C for 96 h to obtain enzymatically hydrolyzed powder fermented material;

[0061] S3. Preparation of polysaccharide complex iron and selenium solution: Mix 10 g of glutinous sorghum, 100 g of Polygonum chinense L., 75 g of Phellinus igniarius, and 15 g of fingered citron evenly to obtain a mixed powder; weigh 100 g of the mixed powder, then add 0.01 g of ferrous fumarate, 0.002 g of nano-selenium, 6 g of cellulase, 8 g of hemicellulase, and 250 g of water, mix evenly, and keep warm at 50 °C for 8 h to obtain polysaccharide complex iron and selenium solution;

[0062] S4. Preparation of polysaccharide complex iron and selenium fermented material: Add 1 g of glucose and 1 mL of Clostridium butyricum bacterial solution to 100 g of polysaccharide complex iron and selenium solution, stir evenly, place in a plastic bag, and ferment at 32 °C for 96 h to obtain polysaccharide complex iron and selenium fermented material;

[0063] S5. Preparation of acidified complex iron and selenium fermented material: Add 10 g of citric acid monohydrate and 250 g of water to 100 g of polysaccharide complex iron and selenium fermented material, keep it warm at 60 °C for 1 h, then add 18 g of lactic acid powder, keep it warm at 60 °C for 3 h, and then dry it in an oven at 60 °C until the moisture content is 17% (w / w) to obtain the acidified complex iron and selenium fermented material;

[0064] S6. Preparation of feed additive: Mix 70 g of enzymolysis powder fermented material, 50 g of acidified complex iron and selenium fermented material and 30 g of water evenly, and ferment at 32 °C for 36 h to obtain the feed additive.

[0065] Example 2

[0066] A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula, comprising the following steps:

[0067] S1. Preparation of enzymolysis primary wet powder: Add wax gourd powder, Ficus hirta Vahl, Stevia rebaudiana Bertoni, and Luffa cylindrica (L.) Roem. extract to water, and the mass ratio of wax gourd powder, Ficus hirta Vahl, Stevia rebaudiana Bertoni, Luffa cylindrica (L.) Roem. extract to water is 2:15:3:20:30, mix evenly to obtain the primary wet powder. Based on the mass of the primary wet powder, then add 5% hemicellulase, 5% cellulase, and 3% pectinase to the primary wet powder, and keep it warm at 40 °C for 2.0 h to obtain the enzymolysis primary wet powder;

[0068] S2. Preparation of enzymolysis powder fermented material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid, and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymolysis primary wet powder obtained in step S1, stir evenly, pack it into a plastic bag, and ferment it sealed at 32 °C for 96 h to obtain the enzymolysis powder fermented material;

[0069] S3. Preparation of polysaccharide complex iron and selenium liquid: Mix 15 g of glutinous sorghum, 100 g of Polygonum chinense L., 70 g of Phellinus igniarius, and 10 g of Citrus medica L. var. sarcodactylis Swingle evenly to obtain a mixed powder; weigh 100 g of the mixed powder, then add 0.01 g of ferrous fumarate, 0.002 g of nano selenium, 6 g of cellulase, 8 g of hemicellulase, and 250 g of water to it, mix evenly, and keep it warm at 50 °C for 8 h to obtain the polysaccharide complex iron and selenium liquid;

[0070] S4. Preparation of polysaccharide complex iron and selenium fermented material: Add 1 g of glucose and 1 mL of Clostridium butyricum liquid to 100 g of the polysaccharide complex iron and selenium liquid, stir evenly, pack it into a plastic bag, and ferment it sealed at 32 °C for 96 h to obtain the polysaccharide complex iron and selenium fermented material;

[0071] S5. Preparation of acidified complex iron-selenium fermented material: Add 10 g of citric acid monohydrate and 250 g of water to 100 g of polysaccharide complex iron-selenium fermented material, keep it warm at 60 °C for 1 h, then add 18 g of lactic acid powder, keep it warm at 60 °C for 3 h, and then dry it in an oven at 60 °C until the moisture content reaches 17% (w / w) to obtain the acidified complex iron-selenium fermented material;

[0072] S6. Preparation of feed additive: Mix 70 g of enzymolysis powder fermented material, 50 g of acidified complex iron-selenium fermented material and 30 g of water evenly, and ferment at 32 °C for 36 h to obtain the feed additive.

[0073] Example 3

[0074] A method for preparing a feed additive for relieving sows' constipation using a traditional Chinese medicine formula, comprising the following steps:

[0075] S1. Preparation of enzymolysis primary wet powder: Add winter melon powder, Ficus hirta Vahl, Stevia rebaudiana Bertoni, and Luffa cylindrica extract to water, where the mass ratio of winter melon powder, Ficus hirta Vahl, Stevia rebaudiana Bertoni, Luffa cylindrica extract to water is 1:15:4:20:20, mix evenly to obtain the primary wet powder. Based on the mass of the primary wet powder, then add 5% hemicellulase, 5% cellulase, and 3% pectinase to the primary wet powder, and keep it warm at 40 °C for 2.0 h to obtain the enzymolysis primary wet powder;

[0076] S2. Preparation of enzymolysis powder fermented material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid, and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymolysis primary wet powder obtained in step S1, stir evenly, put it into a plastic bag, and ferment at 32 °C for 96 h to obtain the enzymolysis powder fermented material;

[0077] S3. Preparation of polysaccharide complex iron-selenium liquid: Mix 15 g of glutinous sorghum, 120 g of Polygonum chinense L., 65 g of Phellinus igniarius, and 10 g of Citrus medica L. var. sarcodactylis Swingle evenly to obtain a mixed powder; weigh 100 g of the mixed powder, then add 0.01 g of ferrous fumarate, 0.002 g of nano-selenium, 6 g of cellulase, 8 g of hemicellulase, and 250 g of water to it, mix evenly, and keep it warm at 50 °C for 8 h to obtain the polysaccharide complex iron-selenium liquid;

[0078] S4. Preparation of polysaccharide complex iron-selenium fermented material: Add 1 g of glucose and 1 mL of Clostridium butyricum liquid to 100 g of the polysaccharide complex iron-selenium liquid, stir evenly, put it into a plastic bag, and ferment at 32 °C for 96 h to obtain the polysaccharide complex iron-selenium fermented material;

[0079] S5. Preparation of acidified compound iron and selenium fermentation material: Add 10 g of citric acid monohydrate and 250 g of water to 100 g of polysaccharide compound iron and selenium fermentation material, keep warm at 60 °C for 1 h, then add 18 g of lactic acid powder, keep warm at 60 °C for 3 h, and then dry in an oven at 60 °C until the moisture content is 17% (w / w) to obtain the acidified compound iron and selenium fermentation material;

[0080] S6. Preparation of feed additive: Mix 60 g of enzymolysis powder fermentation material, 50 g of acidified compound iron and selenium fermentation material and 30 g of water evenly, and ferment at 32 °C for 36 h to obtain the feed additive.

[0081] Example 4

[0082] A method for preparing a feed additive for relieving sows' constipation using a traditional Chinese medicine formula, comprising the following steps:

[0083] S1. Preparation of enzymolysis primary wet powder: Add wax gourd powder, Ficus hirta Vahl, Stevia rebaudiana Bertoni, and Luffa cylindrica (L.) Roem. extract to water, where the mass ratio of wax gourd powder, Ficus hirta Vahl, Stevia rebaudiana Bertoni, Luffa cylindrica (L.) Roem. extract to water is 3:12:3:18:20, mix evenly to obtain the primary wet powder. Based on the mass of the primary wet powder, then add 5% hemicellulase, 5% cellulase, and 3% pectinase to the primary wet powder, and keep warm at 40 °C for 2.0 h to obtain the enzymolysis primary wet powder;

[0084] S2. Preparation of enzymolysis powder fermentation material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid, and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymolysis primary wet powder obtained in step S1, stir evenly, pack into a plastic bag, and ferment sealed at 32 °C for 96 h to obtain the enzymolysis powder fermentation material;

[0085] S3. Preparation of polysaccharide compound iron and selenium liquid: Mix 10 g of glutinous sorghum, 120 g of Polygonum chinense L., 65 g of Phellinus igniarius, and 15 g of Citrus medica L. var. sarcodactylis Swingle evenly to obtain a mixed powder; weigh 100 g of the mixed powder, then add 0.01 g of ferrous fumarate, 0.002 g of nano selenium, 6 g of cellulase, 8 g of hemicellulase, and 250 g of water to it, mix evenly, and keep warm at 50 °C for 8 h to obtain the polysaccharide compound iron and selenium liquid;

[0086] S4. Preparation of polysaccharide compound iron and selenium fermentation material: Add 1 g of glucose and 1 mL of Clostridium butyricum liquid to 100 g of the polysaccharide compound iron and selenium liquid, stir evenly, pack into a plastic bag, and ferment sealed at 32 °C for 96 h to obtain the polysaccharide compound iron and selenium fermentation material;

[0087] S5. Preparation of acidified complex iron and selenium fermentation material: Add 10 g of citric acid monohydrate and 250 g of water to 100 g of polysaccharide complex iron and selenium fermentation material, keep it warm at 60 °C for 1 h, then add 18 g of lactic acid powder, keep it warm at 60 °C for 3 h, and then dry it in an oven at 60 °C until the moisture content reaches 17% (w / w) to obtain the acidified complex iron and selenium fermentation material;

[0088] S6. Preparation of feed additive: Mix 80 g of enzymolysis powder fermentation material, 60 g of acidified complex iron and selenium fermentation material and 40 g of water evenly, and ferment at 32 °C for 36 h to obtain the feed additive.

[0089] Comparative Example 1

[0090] Compared with Example 1, in step S6, mix 70 g of enzymolysis powder fermentation material, 10 g of acidified complex iron and selenium fermentation material and 30 g of water evenly, and ferment at 32 °C for 36 h to obtain the feed additive, and the others are the same as in Example 1.

[0091] Comparative Example 2

[0092] A method for preparing a feed additive for relieving sows' constipation using a traditional Chinese medicine formula, comprising the following steps:

[0093] S1. Preparation of enzymolysis primary wet powder: Add wax gourd powder, five-clawed peach, sweet leaf bacteria, and loofah extract to water, and the mass ratio of wax gourd powder, five-clawed peach, sweet leaf bacteria, loofah extract to water is 2:11:3:16:15, mix evenly to obtain the primary wet powder. Based on the mass of the primary wet powder, then add 5% hemicellulase, 5% cellulase, and 3% pectinase to the primary wet powder, and keep it warm at 40 °C for 2.0 h to obtain the enzymolysis primary wet powder;

[0094] S2. Preparation of enzymolysis powder fermentation material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid, and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymolysis primary wet powder obtained in step S1, stir evenly, put it into a plastic bag, and ferment at 32 °C for 96 h to obtain the enzymolysis powder fermentation material;

[0095] S3. Preparation of polysaccharide complex iron and selenium liquid: Mix 10 g of glutinous sorghum, 100 g of Polygonum chinense, 75 g of Phellinus igniarius, and 15 g of fingered citron evenly to obtain a mixed powder; weigh 100 g of the mixed powder, and then add 0.01 g of ferrous fumarate, 0.002 g of nano-selenium, 6 g of cellulase, 8 g of hemicellulase, and 250 g of water to it, mix evenly, and keep it warm at 50 °C for 8 h to obtain the polysaccharide complex iron and selenium liquid;

[0096] S4. Preparation of polysaccharide complex iron and selenium fermentation material: Add 1 g of glucose and 1 mL of Clostridium butyricum bacterial liquid to 100 g of polysaccharide complex iron and selenium liquid, stir evenly, pack it into a plastic bag, and ferment it sealed at 32 °C for 96 h to obtain the polysaccharide complex iron and selenium fermentation material;

[0097] S5. Preparation of feed additive: Mix 70 g of enzymolysis powder fermentation material, 50 g of polysaccharide complex iron and selenium fermentation material and 30 g of water evenly, and ferment at 32 °C for 36 h to obtain the feed additive.

[0098] Compared with Example 1, the polysaccharide complex iron and selenium fermentation material is not acidified.

[0099] Comparative Example 3

[0100] Compared with Example 1, winter melon powder is not added, and others are the same as Example 1.

[0101] Comparative Example 4

[0102] Compared with Example 1, Ficus hirta Vahl is not added, and others are the same as Example 1.

[0103] Comparative Example 5

[0104] Compared with Example 1, except that Phellinus igniarius is not added, others are the same as Example 1.

[0105] Comparative Example 6

[0106] Compared with Example 1, loofah sponge extract is not added, and others are the same as Example 1.

[0107] Comparative Example 7

[0108] Compared with Example 1, glutinous sorghum is not added, and others are the same as Example 1.

[0109] Comparative Example 8

[0110] Compared with Example 1, Polygonum chinense L. is not added, and others are the same as Example 1.

[0111] The feed additives prepared in Example 1 and Comparative Examples 1-8 were tested to study their effects on sows' constipation. The specific test methods are as follows:

[0112] Select 1000 Duroc×Landrace×Yorkshire sows at 60 days of pregnancy with similar health status and parity, and randomly divide them into 10 groups with 10 replicates in each group and 10 sows in each group. The sows in the control group are fed a basal diet, and the experimental groups are fed the basal diet + 2% feed additive (i.e., the feed additives prepared in Example 1 and Comparative Examples 1-8). The experimental period is 13 days. The composition of the basal diet is corn: soybean meal: concentrated feed (Baiyi Yunzhu concentrated feed, Hunan Baiyi Yunshang Technology Co., Ltd.) at 64:24:12. The statistical data standard is as follows: severe constipation: difficult defecation in pigs, with feces in granular form and hard texture; mild constipation: feces in cylindrical form or with some granules, and relatively hard when stepped on; appropriate feces: easy defecation in sows, with soft feces that can form and disperse when falling to the ground. The statistical results are based on the average of 13 days, and the number of heads = the sum of the number of sows with constipation per day / 13. The test results are shown in Table 1:

[0113] Table 1

[0114] Group Total number of heads Number of heads with severe constipation Number of heads with mild constipation Number of heads with moderate feces Control group 100 46 42 12 Example 1 100 15 33 52 Comparative Example 1 100 22 42 36 Comparative Example 2 100 26 44 30 Comparative Example 3 100 18 33 49 Comparative Example 4 100 17 31 52 Comparative Example 5 100 17 32 51 Comparative Example 6 100 18 32 50 Comparative Example 7 100 18 35 47 Comparative Example 8 100 21 41 38

[0115] Finally, it should be noted that the above embodiments do not limit the present invention in any form. For those skilled in the art, based on the present invention, some modifications and improvements can be made. Therefore, any modification or improvement made without departing from the spirit of the present invention falls within the scope of protection required by the present invention.

Claims

1. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula, characterized in that, It includes the following steps: S1. Preparation of enzymatically hydrolyzed primary wet powder: Add wax gourd powder, Ficus simplicissima Lour., Stevia rebaudiana, and loofah sponge extract into water, mix evenly to obtain primary wet powder. Then add hemicellulase, cellulase, and pectinase to the primary wet powder for enzymatic hydrolysis reaction to obtain enzymatically hydrolyzed primary wet powder; S2. Preparation of enzymatically hydrolyzed powder fermentation material: Add Saccharomyces cerevisiae liquid, Clostridium butyricum liquid, and Lactobacillus plantarum liquid to the enzymatically hydrolyzed primary wet powder obtained in step S1, stir evenly, and ferment in a sealed manner to obtain enzymatically hydrolyzed powder fermentation material; S3. Preparation of polysaccharide complex iron and selenium solution: Mix glutinous sorghum, Polygonum chinense L., Phellinus igniarius, and fingered citron evenly to obtain a mixed powder. Then add ferrous fumarate, nano-selenium, cellulase, hemicellulase, and water, mix evenly, and keep warm at 45 - 60 °C for 6 - 10 h to obtain polysaccharide complex iron and selenium solution; S4. Preparation of polysaccharide complex iron and selenium fermentation material: Add glucose and Clostridium butyricum liquid to the polysaccharide complex iron and selenium solution, stir evenly, and ferment in a sealed manner to obtain polysaccharide complex iron and selenium fermentation material; S5. Preparation of acidified complex iron and selenium fermentation material: Add citric acid monohydrate and water to the polysaccharide complex iron and selenium fermentation material, keep warm at 55 - 65 °C for 0.5 - 2 h, then add lactic acid powder, continue to keep warm for 2 - 4 h, and dry to obtain acidified complex iron and selenium fermentation material; S6. Preparation of feed additive: Mix the enzymatically hydrolyzed powder fermentation material, acidified complex iron and selenium fermentation material, and water evenly, and ferment to obtain the feed additive.

2. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula according to claim 1, characterized in that, In step S1, the mass ratio of wax gourd powder, Ficus simplicissima Lour., Stevia rebaudiana, loofah sponge extract, and water is 1 - 3:10 - 15:2 - 4:15 - 20:15 - 30.

3. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula according to claim 1, characterized in that, In step S1, based on the mass of the primary wet powder, the addition amount of hemicellulase is 5%, the addition amount of cellulase is 5%, and the addition amount of pectinase is 3%; The temperature of the enzymatic hydrolysis reaction is 38 - 42 °C, and the time of the enzymatic hydrolysis reaction is 1 - 3 h.

4. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula according to claim 1, characterized in that, In step S2, based on the mass of the enzymatically hydrolyzed primary wet powder, the addition amount of Saccharomyces cerevisiae liquid is 1% (v / w), the addition amount of Clostridium butyricum liquid is 1% (v / w), and the addition amount of Lactobacillus plantarum liquid is 1% (v / w).

5. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula, characterized in that, In step S2, the temperature of the sealed fermentation is 30 - 33 °C, and the time of the sealed fermentation is 72 - 144 h.

6. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula according to claim 1, characterized in that, In step S3, the mass ratio of glutinous sorghum, Polygonum chinense L., Phellinus igniarius, and fingered citron is 1 - 3:18 - 25:12 - 16:2 - 5; the mass ratio of ferrous fumarate, nano-selenium, cellulase, hemicellulase, water, and the mixed powder is 0.01:0.002:6:8:250:

100.

7. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula according to claim 1, characterized in that, In step S4, based on the mass of the polysaccharide complex iron and selenium solution, the addition amount of glucose is 1% (w / w), and the addition amount of Clostridium butyricum liquid is 1% (v / w); The temperature of the sealed fermentation is 30 - 33 °C, and the time of the sealed fermentation is 72 - 144 h.

8. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula according to claim 1, characterized in that, In step S5, the mass ratio of citric acid monohydrate, water, polysaccharide complex iron and selenium fermentation material, and lactic acid powder is 8 - 15:200 - 300:100:15 - 20.

9. A method for preparing a feed additive for relieving constipation in sows using a traditional Chinese medicine formula, characterized in that, In step S6, the mass ratio of the enzymatically hydrolyzed powder fermentation material, acidified complex iron and selenium fermentation material, and water is 6 - 8:4 - 6:2 - 4; The fermentation temperature is 30 - 33 °C, and the sealed fermentation time is 24 - 48 h.

10. A feed additive prepared by the method according to any one of claims 1 - 9.