Feed additive for reducing constipation of sows as well as preparation method and application of feed additive

Abstract

The invention provides a preparation method of a feed additive for reducing constipation of sows. The preparation method comprises the following steps: preparing an enzymolysis powder fermentation material; preparing a polysaccharide composite iron liquid fermentation material; carrying out acid treatment on the polysaccharide composite iron liquid fermentation material; and preparing a functional powder fermentation material, and carrying out mixed fermentation on the enzymolysis powder fermentation material, the acid-treated polysaccharide composite iron liquid fermentation material and the functional powder fermentation material to obtain the feed additive. The prepared feed additive for relieving constipation of sows is prepared through a novel compound acid pretreatment process, bacterium and enzyme synergistic fermentation and a novel formula, and has innovativeness and huge market space.

Description

Technical Field

[0001] The present invention belongs to the technical field of feed additives for pig breeding, and particularly relates to a feed additive for reducing constipation in sows, a preparation method thereof, and an application thereof. Background Art

[0002] Pigs are omnivorous mammals, with a stout body, short limbs, a relatively long nose and snout, docile temperament, strong adaptability, fast reproduction. They can mate 5 - 12 months after birth and have an average lifespan of 20 years.

[0003] During the breeding process of pigs, the constipation problem in sows has always been a stubborn problem. Constipation in sows not only affects the nutrient absorption of sows, but also affects the number of litters, the average litter weight, the number of healthy piglets, etc. Therefore, alleviating constipation in sows is a key link in improving the breeding efficiency of sows. Using natural plants to produce functional feed additives for feed has the characteristics of multiple action targets, remarkable effects, no toxic side effects, safety and greenness.

[0004] Chinese Patent CN105309752A discloses a Chinese herbal medicine feed additive for preventing and treating constipation in pregnant sows. The feed additive is composed of astragalus membranaceus, codonopsis pilosula, angelica sinensis, and ophiopogon japonicus as raw materials, and the weight ratio of astragalus membranaceus, codonopsis pilosula, angelica sinensis, and ophiopogon japonicus is 3.5 - 4.5:2.5 - 3.5:1.5 - 2.5:1. The feed additive of this invention is composed only of traditional Chinese medicine raw materials according to the formula theory of traditional Chinese veterinary medicine and is non-toxic. Astragalus membranaceus is warm in nature and is an important medicine for replenishing qi. Codonopsis pilosula nourishes yin and replenishes vital energy. Angelica sinensis can effectively generate blood, and ophiopogon japonicus can increase the body fluid of sows and effectively supplement the water in the intestine. The four medicines are formulated according to the ratio, which can effectively supplement the qi and blood of sows, promote the intestinal motility of sows, and keep the water in the intestine balanced. Make the sows have sufficient qi and blood. Thus, it can fundamentally prevent constipation in sows without side effects and is a truly green feed additive.

[0005] The above patent only uses simple Chinese herbal medicines to prepare feed additives, mainly for prevention, and needs to be eaten continuously. Therefore, through scientific formulation, a new pretreatment process of a new composite acid, combined fermentation of bacteria and enzymes, and a new formulation, to produce a feed additive that can quickly reduce constipation in sows, has innovation and a huge market space. Summary of the Invention

[0006] Based on the technical problems existing in the background art, the present invention provides a feed additive for reducing constipation in sows, a preparation method thereof, and an application thereof. Using traditional Chinese medicine as the raw material for fermentation, and using a variety of bacteria and enzymes for combined fermentation, through a new traditional Chinese medicine formulation and a new process, to produce a feed additive that can quickly reduce constipation in sows.

[0007] In order to achieve the above object, the technical solution of the present invention is as follows:

[0008] A preparation method of a feed additive for reducing constipation in sows, comprising the following steps:

[0009] (1) Preparation of enzymolysis powder fermentation material: Mix glossy privet fruit powder, pomelo peel powder, sweet potato powder, and pumpkin seed powder in water to obtain a primary wet powder; add a first complex enzyme to the primary wet powder and keep it at 45 - 55 °C for 2 - 4 h to obtain an enzymolyzed primary wet powder;

[0010] Add Saccharomyces cerevisiae liquid, Clostridium butyricum liquid, and Lactobacillus plantarum liquid to the enzymolyzed primary wet powder, stir, and after stirring evenly, ferment it sealed at 30 - 35 °C for 90 - 102 h to obtain an enzymolysis powder fermentation material;

[0011] (2) Preparation of polysaccharide complex iron liquid fermentation material: Mix konjac powder, lotus root powder, and pumpkin seed powder to obtain a mixed medicinal powder, add ferrous fumarate and sterilized water to the mixed medicinal powder, stir evenly, and keep it at 35 - 45 °C for 3 - 5 h to obtain a polysaccharide complex iron liquid;

[0012] Add glucose and Clostridium butyricum liquid to the obtained polysaccharide complex iron liquid, stir evenly, and ferment it sealed at 30 - 35 °C for 90 - 102 h to obtain a polysaccharide complex iron liquid fermentation material;

[0013] (3) Preparation of a compound iron fermentation material solution with acid secondary treatment: Add citric acid monohydrate and sterilized water to the polysaccharide complex iron liquid fermentation material obtained in step (2), keep it at 55 - 65 °C for 1 - 3 h to obtain a compound iron fermentation material solution with acid primary treatment, then add lactic acid powder and keep it at 55 - 65 °C for 1 - 3 h to obtain a compound iron fermentation material solution with acid secondary treatment;

[0014] (4) Preparation of a functional powder fermentation material: Mix tea powder and kiwifruit powder, then add cellulase, hemicellulase, and sterilized water, and keep it at 45 - 55 °C for 1 - 3 h to obtain a functional powder enzymolysis material;

[0015] Add glucose, peptone, Saccharomyces cerevisiae liquid, and Lactobacillus plantarum liquid to the functional powder enzymolysis material, stir evenly, and ferment it sealed at 30 - 35 °C for 90 - 102 h to obtain a functional powder fermentation material;

[0016] (5) Preparation of the feed additive: Mix and ferment the enzymolysis powder fermentation material obtained in step (1), the compound iron fermentation material solution with acid secondary treatment obtained in step (3), and the functional powder fermentation material obtained in step (4). After fermentation is completed, obtain the feed additive.

[0017] Preferably, in step (1), the mass ratio of glossy privet fruit powder, pomelo peel powder, sweet potato powder, pumpkin seed powder, and water is 0.1 - 0.3:6 - 10:1 - 5:0.01 - 0.2:6 - 9.

[0018] Preferably, in step (1), the first complex enzyme is composed of hemicellulase, cellulase, and pectinase, and the dosages are 1%, 2%, and 3% of the mass of the primary wet powder, respectively.

[0019] Preferably, in step (1), based on the mass of the enzymatically hydrolyzed primary wet powder, the addition amount of the Saccharomyces cerevisiae liquid is 1% (v / w), the addition amount of the Clostridium butyricum liquid is 1% (v / w), and the addition amount of the Lactobacillus plantarum liquid is 1% (v / w).

[0020] Preferably, in step (2), the mass ratio of konjac powder, lotus root powder, and pumpkin seed powder is 3 - 7:1 - 5:0.1 - 2;

[0021] The dosages of ferrous fumarate and sterilized water are 0.01% and 250% of the mass of the mixed medicinal powder, respectively;

[0022] Based on the mass of the polysaccharide complex iron liquid, the addition amount of glucose is 1% (w / w), and the addition amount of the Clostridium butyricum liquid is 1% (v / w).

[0023] Preferably, in step (3), the dosages of citric acid monohydrate and sterilized water are 10% and 250% of the mass of the polysaccharide complex iron liquid fermentation material, respectively;

[0024] The dosage of lactic acid powder is 25% of the mass of the composite iron fermentation liquid after the first acid treatment.

[0025] Preferably, in step (4), the mixed mass ratio of tea powder and kiwifruit powder is 7 - 11:0.1 - 2;

[0026] The dosages of cellulase, hemicellulase, and sterilized water are 1%, 4%, and 70% of the total mass of tea powder and kiwifruit powder, respectively;

[0027] Based on the mass of the functional powder enzymatically hydrolyzed material, the addition amount of glucose is 1% (w / w), the addition amount of peptone is 1% (w / w), the addition amount of the Saccharomyces cerevisiae liquid is 1% (v / w), and the addition amount of the Lactobacillus plantarum liquid is 1% (v / w).

[0028] Preferably, in step (5), the mass ratio of the enzymatically hydrolyzed powder fermentation material, the composite iron fermentation liquid after the second acid treatment, and the functional powder fermentation material is 4 - 6:0.1 - 2:2 - 4;

[0029] The fermentation temperature is 30 - 35 °C, and the fermentation time is 20 - 28 h.

[0030] In the second aspect, the present invention also claims protection for the feed additive prepared by the above preparation method.

[0031] In the third aspect, the present invention also claims protection for the application of the above feed additive in reducing constipation in sows.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] The feed additive provided by the present invention is prepared from eight raw materials, namely, glossy privet fruit, pumpkin seed powder, konjac powder, tea powder, grapefruit peel, lotus root powder, sweet potato powder, and kiwi fruit, which can synergistically exert the maximum effect of reducing sow constipation. At the same time, in the preparation process, a multi-bacteria pretreatment process of multiple composite acids and composite fermentation are adopted, which can enhance resistance, promote nutrient absorption, and improve gastrointestinal flora, so that the prepared feed additive can exert the best medicinal effect of alleviating constipation.

[0034] The present invention produces a feed additive for relieving sow constipation through a new composite acid pretreatment process, bacterial enzyme synergistic fermentation, and a new formula, which is innovative and has huge market potential. DETAILED DESCRIPTION

[0035] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] Unless otherwise specified, all commodities or reagents in this invention were purchased through market channels.

[0037] Among them, Saccharomyces cerevisiae, Clostridium butyricum, and Lactobacillus plantarum were purchased from Qingdao Weilan Biotechnology Co., Ltd.

[0038] Cellulase was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., 11000 U / g;

[0039] Hemicellulase was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., 50,000 U / g;

[0040] Pectinase was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., H type / viscosity reduction, 25000U / g;

[0041] Ligustrum lucidum fruit was purchased from Beijing Tong Ren Tang;

[0042] Pumpkin seed powder was purchased from Xuzhou Nature Food Co., Ltd.

[0043] Konjac flour was purchased from Hunan Century Huaxing Bioengineering Co., Ltd.;

[0044] Tea powder was purchased from Yibeixiang Tea Industry Cooperative in Shengzhou, Zhejiang Province;

[0045] Grapefruit peel was purchased from Guangdong Tongmei Food Co., Ltd.;

[0046] The lotus root starch was purchased from Yihetang Helian Industry Development (Hubei) Co., Ltd.

[0047] The kiwifruit powder was purchased from Ningxia Lannuo Biotechnology Co., Ltd.;

[0048] The sweet potato powder was purchased from Anqing Liangsi Yiyou Food Co., Ltd.;

[0049] The peptone was purchased from Jinan Lanjue Trading Co., Ltd.;

[0050] The glucose was purchased from Shandong Xujie Chemical Industry Co., Ltd.;

[0051] The ferrous fumarate was purchased from Nanning Zeweier Feed Co., Ltd.;

[0052] The citric acid monohydrate was purchased from Shanghai Mingjiong Biotechnology Co., Ltd.;

[0053] The lactic acid powder was purchased from Qingdao Huazhiyuan Food Feed Additive Co., Ltd.

[0054] In each example and comparative example of the present invention, the culture medium was prepared as follows:

[0055] Yeast extract 1% (w / w), peptone 1% (w / w), glucose 4% (w / w); after preparation, it was sterilized at 115 °C for 30 min and cooled to room temperature.

[0056] In each example and comparative example of the present invention, the activation of the bacterial liquid was prepared as follows:

[0057] From the Clostridium butyricum strain preserved on the slant, six loops of bacteria were scraped with an inoculation loop (loop diameter 0.3 mm) and inoculated into 300 mL of activation medium, placed in a 500 mL Erlenmeyer flask, cultured at 33 °C, rotation speed 180 r / m, and the yeast was cultured for 22 h to obtain the Clostridium butyricum liquid.

[0058] From the Saccharomyces cerevisiae strain preserved on the slant, six loops of bacteria were scraped with an inoculation loop (loop diameter 0.3 mm) and inoculated into 300 mL of activation medium, placed in a 500 mL Erlenmeyer flask, cultured at 33 °C, rotation speed 180 r / m, and the yeast was cultured for 18 h to obtain the Saccharomyces cerevisiae liquid.

[0059] From the Lactobacillus plantarum strain preserved on the slant, six loops of bacteria were scraped with an inoculation loop (loop diameter 0.3 mm) and inoculated into 300 mL of activation medium, placed in a 500 mL Erlenmeyer flask, cultured at 33 °C, rotation speed 180 r / m, and the yeast was cultured for 18 h to obtain the Lactobacillus plantarum liquid.

[0060] Before preparation: Fructus Ligustri Lucidi, pumpkin seed powder, konjac powder, tea powder, pomelo peel, lotus root starch, sweet potato powder, and kiwifruit were respectively pulverized with a pulverizer, and after pulverization, they were passed through an 80-mesh sieve.

[0061] Example 1

[0062] A preparation method of a feed additive for reducing constipation in sows, comprising the following steps:

[0063] (1) Preparation of enzymatically hydrolyzed powder fermented material: Mix fructus ligustri lucidi powder, pomelo peel powder, sweet potato powder, pumpkin seed powder and water in a mass ratio of 0.2:8:3:0.1:8 to obtain a primary wet powder; add hemicellulase, cellulase and pectinase to the primary wet powder, with the dosages being 1%, 2% and 3% of the mass of the primary wet powder respectively, and keep warm at 50 °C for 3 h to obtain an enzymatically hydrolyzed primary wet powder;

[0064] Based on the mass of the enzymatically hydrolyzed primary wet powder, add 1% (v / w) of Saccharomyces cerevisiae liquid, 1% (v / w) of Clostridium butyricum liquid and 1% (v / w) of Lactobacillus plantarum liquid to the enzymatically hydrolyzed primary wet powder, stir, and after stirring evenly, ferment in a sealed manner at 32 °C for 96 h to obtain an enzymatically hydrolyzed powder fermented material;

[0065] (2) Preparation of polysaccharide complex iron liquid fermented material: Mix konjac powder, lotus root powder and pumpkin seed powder in a mass ratio of 5:3:1 to obtain a mixed medicinal powder, add 0.01% of ferrous fumarate and 250% of sterilized water based on the mass of the mixed medicinal powder, stir evenly, and keep warm at 40 °C for 4 h to obtain a polysaccharide complex iron liquid;

[0066] Based on the mass of the polysaccharide complex iron liquid, add 1% (w / w) of glucose and 1% (v / w) of Clostridium butyricum liquid to the obtained polysaccharide complex iron liquid, stir evenly, and ferment in a sealed manner at 32 °C for 96 h to obtain a polysaccharide complex iron liquid fermented material;

[0067] (3) Preparation of the composite iron fermented material liquid with acid secondary treatment: Add citric acid monohydrate and sterilized water to the polysaccharide complex iron liquid fermented material obtained in step (2), keep warm at 60 °C for 2 h to obtain a composite iron fermented material liquid with acid primary treatment, then add lactic acid powder and keep warm at 60 °C for 2 h to obtain a composite iron fermented material liquid with acid secondary treatment;

[0068] Among them, the dosages of citric acid monohydrate and sterilized water are 10% and 250% of the mass of the polysaccharide complex iron liquid fermented material respectively; the dosage of lactic acid powder is 25% of the mass of the composite iron fermented material liquid with acid primary treatment.

[0069] (4) Preparation of the functional powder fermented material: Mix tea powder and kiwifruit powder in a mass ratio of 9:1, then add 1% of cellulase, 4% of hemicellulase and 70% of sterilized water based on the total mass of the tea powder and kiwifruit powder, and keep warm at 50 °C for 2 h to obtain a functional powder enzymatically hydrolyzed material;

[0070] Based on the mass of the functional powder enzymolysis material, add 1% (w / w) glucose, 1% (w / w) peptone, 1% (v / w) Saccharomyces cerevisiae liquid, and 1% (v / w) Lactobacillus plantarum liquid to the functional powder enzymolysis material. After stirring evenly, ferment at 32°C in a sealed condition for 96 h to obtain the functional powder fermentation material;

[0071] (5) Preparation of feed additive: Mix the enzymolysis powder fermentation material obtained in step (1), the composite iron fermentation material liquid after acid secondary treatment obtained in step (3), and the functional powder fermentation material obtained in step (4) according to a mass ratio of 5:1:3, and ferment at 32°C for 24 h to obtain the feed additive.

[0072] Example 2

[0073] A preparation method of a feed additive for reducing sows' constipation, comprising the following steps:

[0074] (1) Preparation of enzymolysis powder fermentation material: Mix glossy privet fruit powder, pomelo peel powder, sweet potato powder, pumpkin seed powder and water according to a mass ratio of 0.3:10:5:0.2:9 to obtain the primary wet powder; add hemicellulase, cellulase, and pectinase to the primary wet powder, with the dosages being 1%, 2%, and 3% of the mass of the primary wet powder respectively, and keep warm at 50°C for 3 h to obtain the enzymolyzed primary wet powder;

[0075] Based on the mass of the enzymolyzed primary wet powder, add 1% (v / w) Saccharomyces cerevisiae liquid, 1% (v / w) Clostridium butyricum liquid, and 1% (v / w) Lactobacillus plantarum liquid to the enzymolyzed primary wet powder, stir, and after stirring evenly, ferment at 32°C in a sealed condition for 96 h to obtain the enzymolysis powder fermentation material;

[0076] (2) Preparation of polysaccharide complex iron liquid fermentation material: Mix konjac powder, lotus root powder, and pumpkin seed powder according to a mass ratio of 7:5:2 to obtain the mixed medicinal powder, add 0.01% ferrous fumarate and 250% sterilized water based on the mass of the mixed medicinal powder, stir evenly, and keep warm at 40°C for 4 h to obtain the polysaccharide complex iron liquid;

[0077] Based on the mass of the polysaccharide complex iron liquid, add 1% (w / w) glucose and 1% (v / w) Clostridium butyricum liquid to the obtained polysaccharide complex iron liquid, stir evenly, and ferment at 32°C in a sealed condition for 96 h to obtain the polysaccharide complex iron liquid fermentation material;

[0078] (3) Preparation of acid secondary-treated composite iron fermentation material liquid: Add citric acid monohydrate and sterilized water to the polysaccharide complex iron liquid fermentation material obtained in step (2), keep warm at 60°C for 2 h to obtain the acid primary-treated composite iron fermentation material liquid, and then add lactic acid powder and keep warm at 60°C for 2 h to obtain the acid secondary-treated composite iron fermentation material liquid;

[0079] Among them, the dosages of citric acid monohydrate and sterilized water are 10% and 250% of the mass of the polysaccharide complex iron liquid fermentation material respectively; the dosage of lactic acid powder is 25% of the mass of the complex iron fermentation material liquid after the first acid treatment.

[0080] (4) Preparation of the functional powder fermentation material: Mix tea powder and kiwifruit powder according to a mass ratio of 11:2, then add cellulase at 1% of the total mass of tea powder and kiwifruit powder, hemicellulase at 4%, and 70% of sterilized water, and keep warm at 50 °C for 2 h to obtain the functional powder enzymolysis material;

[0081] Based on the mass of the functional powder enzymolysis material, add 1% (w / w) of glucose, 1% (w / w) of peptone, 1% (v / w) of Saccharomyces cerevisiae liquid, and 1% (v / w) of Lactobacillus plantarum liquid to the functional powder enzymolysis material, stir evenly, and ferment sealed at 32 °C for 96 h to obtain the functional powder fermentation material;

[0082] (5) Preparation of the feed additive: Mix the enzymolysis powder fermentation material obtained in step (1), the complex iron fermentation material liquid after the second acid treatment obtained in step (3), and the functional powder fermentation material obtained in step (4) according to a mass ratio of 6:2:4, and ferment at 32 °C for 24 h to obtain the feed additive.

[0083] Example 3

[0084] A preparation method of a feed additive for reducing sows' constipation includes the following steps:

[0085] (1) Preparation of the enzymolysis powder fermentation material: Mix glossy privet fruit powder, pomelo peel powder, sweet potato powder, pumpkin seed powder and water according to a mass ratio of 0.1:6:1.5:0.05:6 to obtain the primary wet powder; add hemicellulase, cellulase, and pectinase to the primary wet powder, and the dosages are 1%, 2%, and 3% of the mass of the primary wet powder respectively, and keep warm at 50 °C for 3 h to obtain the enzymolyzed primary wet powder;

[0086] Based on the mass of the enzymolyzed primary wet powder, add 1% (v / w) of Saccharomyces cerevisiae liquid, 1% (v / w) of Clostridium butyricum liquid, and 1% (v / w) of Lactobacillus plantarum liquid to the enzymolyzed primary wet powder, stir, and after stirring evenly, ferment sealed at 32 °C for 96 h to obtain the enzymolysis powder fermentation material;

[0087] (2) Preparation of the polysaccharide complex iron liquid fermentation material: Mix konjac powder, lotus root powder, and pumpkin seed powder according to a mass ratio of 3:1.5:0.15 to obtain the mixed medicinal powder, add 0.01% of ferrous fumarate and 250% of sterilized water to the mixed medicinal powder, stir evenly, and keep warm at 40 °C for 4 h to obtain the polysaccharide complex iron liquid;

[0088] Based on the mass of the polysaccharide complex iron solution, add 1% (w / w) glucose and 1% (v / w) Clostridium butyricum bacterial solution to the obtained polysaccharide complex iron solution, stir evenly, and ferment in a sealed manner at 32 °C for 96 h to obtain a fermented material of the polysaccharide complex iron solution;

[0089] (3) Preparation of the composite iron fermented material solution with secondary acid treatment: Add citric acid monohydrate and sterilized water to the fermented material of the polysaccharide complex iron solution obtained in step (2), keep it warm at 60 °C for 2 h to obtain a composite iron fermented material solution with primary acid treatment, and then add lactic acid powder and keep it warm at 60 °C for 2 h to obtain a composite iron fermented material solution with secondary acid treatment;

[0090] Among them, the dosages of citric acid monohydrate and sterilized water are 10% and 250% of the mass of the fermented material of the polysaccharide complex iron solution respectively; the dosage of lactic acid powder is 25% of the mass of the composite iron fermented material solution with primary acid treatment.

[0091] (4) Preparation of the functional powder fermented material: Mix tea powder and kiwifruit powder according to a mass ratio of 7:0.5, then add 1% cellulase, 4% hemicellulase and 70% sterilized water based on the total mass of the tea powder and kiwifruit powder, and keep it warm at 50 °C for 2 h to obtain a functional powder enzymolysis material;

[0092] Based on the mass of the functional powder enzymolysis material, add 1% (w / w) glucose, 1% (w / w) peptone, 1% (v / w) Saccharomyces cerevisiae bacterial solution, and 1% (v / w) Lactobacillus plantarum bacterial solution to the functional powder enzymolysis material, stir evenly, and ferment in a sealed manner at 32 °C for 96 h to obtain a functional powder fermented material;

[0093] (5) Preparation of the feed additive: Mix the enzymolysis powder fermented material obtained in step (1), the composite iron fermented material solution with secondary acid treatment obtained in step (3), and the functional powder fermented material obtained in step (4) according to a mass ratio of 4:0.15:2, and ferment at 32 °C for 24 h to obtain the feed additive.

[0094] Comparative Example 1

[0095] Compared with Example 1, in step (5), except that the mass ratio of the enzymolysis powder fermented material, the composite iron fermented material solution with secondary acid treatment, and the functional powder fermented material is changed to 5:0.5:3 for mixed fermentation, the others are the same as in Example 1.

[0096] Comparative Example 2

[0097] A preparation method of a feed additive for reducing sows' constipation, comprising the following steps:

[0098] (1) Preparation of enzymolysis powder fermentation material: Mix glossy privet fruit powder, pomelo peel powder, sweet potato powder, pumpkin seed powder and water according to a mass ratio of 0.2:8:3:0.1:8 to obtain primary wet powder; add hemicellulase, cellulase and pectinase to the primary wet powder, and the dosages are 1%, 2% and 3% of the mass of the primary wet powder respectively, and keep warm at 50 °C for 3 h to obtain enzymolyzed primary wet powder;

[0099] Based on the mass of the enzymolyzed primary wet powder, add 1% (v / w) of Saccharomyces cerevisiae liquid, 1% (v / w) of Clostridium butyricum liquid, and 1% (v / w) of Lactobacillus plantarum liquid to the enzymolyzed primary wet powder, stir, and after stirring evenly, ferment in a sealed manner at 32 °C for 96 h to obtain enzymolysis powder fermentation material;

[0100] (2) Preparation of polysaccharide complex iron liquid fermentation material: Mix konjac powder, lotus root powder and pumpkin seed powder according to a mass ratio of 5:3:1 to obtain a mixed medicinal powder, add 0.01% of ferrous fumarate and 250% of sterilized water based on the mass of the mixed medicinal powder, stir evenly, and keep warm at 40 °C for 4 h to obtain polysaccharide complex iron liquid;

[0101] Based on the mass of the polysaccharide complex iron liquid, add 1% (w / w) of glucose and 1% (v / w) of Clostridium butyricum liquid to the obtained polysaccharide complex iron liquid, stir evenly, and ferment in a sealed manner at 32 °C for 96 h to obtain polysaccharide complex iron liquid fermentation material;

[0102] (3) Preparation of functional powder fermentation material: Mix tea powder and kiwifruit powder according to a mass ratio of 9:1, and then add 1% of cellulase, 4% of hemicellulase and 70% of sterilized water based on the total mass of the tea powder and kiwifruit powder, and keep warm at 50 °C for 2 h to obtain functional powder enzymolysis material;

[0103] Based on the mass of the functional powder enzymolysis material, add 1% (w / w) of glucose, 1% (w / w) of peptone, 1% (v / w) of Saccharomyces cerevisiae liquid, and 1% (v / w) of Lactobacillus plantarum liquid to the functional powder enzymolysis material, stir evenly, and ferment in a sealed manner at 32 °C for 96 h to obtain functional powder fermentation material;

[0104] (4) Preparation of feed additive: Mix the enzymolysis powder fermentation material obtained in step (1), the polysaccharide complex iron liquid fermentation material obtained in step (2) and the functional powder fermentation material obtained in step (3) according to a mass ratio of 5:0.21:3, and ferment at 32 °C for 24 h to obtain feed additive.

[0105] Comparative Example 3

[0106] Compared with Example 1, except that pumpkin seed powder is not added in step (1), the others are the same as in Example 1.

[0107] Comparative Example 4

[0108] Compared with Example 1, except for not adding pomelo peel powder, the others are the same as Example 1.

[0109] Comparative Example 5

[0110] Compared with Example 1, except for not adding konjac powder, the others are the same as Example 1.

[0111] Comparative Example 6

[0112] Compared with Example 1, except for not adding pectinase in step (1), the others are the same as Example 1.

[0113] The feed additives prepared in Example 1 and Comparative Examples 1-6 were tested to study their effects on sows' constipation. The specific test methods are as follows:

[0114] Four hundred Duroc×Landrace×Yorkshire sows at 60 days of gestation with similar health conditions and parities were selected and randomly divided into 8 groups, with 5 replicates in each group and 10 sows in each group. The sows in the control group were fed a basal diet, and the experimental groups were fed the basal diet + 2% feed additive (i.e., the feed additives prepared in Example 1 and Comparative Examples 1-6). The experimental period was 13 days. The composition of the basal diet was corn: soybean meal: concentrated feed (Baiyi Yunzhu Concentrated Feed, Hunan Baiyi Yunshang Technology Co., Ltd.) at 64:24:12. The statistical data standard was as follows: severe constipation: difficult defecation in pigs, with feces in granular form and hard texture; mild constipation: feces in cylindrical form or with some particles, and hard when stepped on; no constipation: easy defecation in sows, with soft feces that can form and disperse when falling to the ground. The statistical results were based on the average of 13 days, and the number of heads = the sum of the number of sows with constipation per day / 13. The test results are shown in Table 1:

[0115] Table 1

[0116]

[0117] It can be seen that through the combination of the traditional Chinese medicine composition and the fermentation process, the obtained feed additive of the present invention can effectively relieve sows' constipation.

[0118] The basic concepts have been described above. Obviously, for those skilled in the art, the above detailed disclosure is only an example and does not constitute a limitation to this specification. Although not explicitly stated here, those skilled in the art may make various modifications, improvements, and deviations to this specification. Such modifications, improvements, and deviations are proposed in this specification, so such modifications, improvements, and deviations still belong to the spirit and scope of the exemplary embodiments of this specification.

[0119] It should be noted that if there are any inconsistencies or conflicts between the descriptions, definitions, and / or uses of terms in the supplementary materials of this specification and the content described in this specification, the descriptions, definitions, and / or uses of terms in this specification shall prevail.

[0120] Finally, it should be understood that the embodiments described in this specification are only used to illustrate the principles of the embodiments of this specification. Other variations may also fall within the scope of this specification. Therefore, by way of example and not limitation, alternative configurations of the embodiments of this specification may be regarded as consistent with the teachings of this specification. Accordingly, the embodiments of this specification are not limited to those explicitly presented and described in this specification.

Claims

1. A preparation method of a feed additive for reducing constipation in sows, characterized in that: It includes the following steps: (1) Preparation of enzymatically hydrolyzed powder fermentation material: Mix glossy privet fruit powder, pomelo peel powder, sweet potato powder, and pumpkin seed powder in water to obtain primary wet powder; add the first complex enzyme to the primary wet powder and keep it at 45 - 55 °C for 2 - 4 h to obtain enzymatically hydrolyzed primary wet powder; Add Saccharomyces cerevisiae liquid, Clostridium butyricum liquid, and Lactobacillus plantarum liquid to the enzymatically hydrolyzed primary wet powder, stir, and after stirring evenly, ferment it sealed at 30 - 35 °C for 90 - 102 h to obtain enzymatically hydrolyzed powder fermentation material; (2) Preparation of polysaccharide complex iron liquid fermentation material: Mix konjac powder, lotus root powder, and pumpkin seed powder to obtain mixed medicinal powder, add ferrous fumarate and sterilized water to the mixed medicinal powder, stir evenly, and keep it at 35 - 45 °C for 3 - 5 h to obtain polysaccharide complex iron liquid; Add glucose and Clostridium butyricum liquid to the obtained polysaccharide complex iron liquid, stir evenly, and ferment it sealed at 30 - 35 °C for 90 - 102 h to obtain polysaccharide complex iron liquid fermentation material; (3) Preparation of compound iron fermentation material liquid with acid secondary treatment: Add citric acid monohydrate and sterilized water to the polysaccharide complex iron liquid fermentation material obtained in step (2), keep it at 55 - 65 °C for 1 - 3 h to obtain compound iron fermentation material liquid with acid primary treatment, then add lactic acid powder and keep it at 55 - 65 °C for 1 - 3 h to obtain compound iron fermentation material liquid with acid secondary treatment; (4) Preparation of functional powder fermentation material: Mix tea powder and kiwifruit powder, then add cellulase, hemicellulase, and sterilized water, keep it at 45 - 55 °C for 1 - 3 h to obtain functional powder enzymatically hydrolyzed material; Add glucose, peptone, Saccharomyces cerevisiae liquid, and Lactobacillus plantarum liquid to the functional powder enzymatically hydrolyzed material, stir evenly, and ferment it sealed at 30 - 35 °C for 90 - 102 h to obtain functional powder fermentation material; (5) Preparation of feed additive: Mix and ferment the enzymatically hydrolyzed powder fermentation material obtained in step (1), the compound iron fermentation material liquid with acid secondary treatment obtained in step (3), and the functional powder fermentation material obtained in step (4). After fermentation is completed, obtain the feed additive.

2. The preparation method of a feed additive for reducing sows' constipation according to claim 1, characterized in that: In step (1), the mass ratio of glossy privet fruit powder, pomelo peel powder, sweet potato powder, pumpkin seed powder to water is 0.1 - 0.3:6 - 10:1 - 5:0.01 - 0.2:6 - 9.

3. The preparation method of a feed additive for reducing constipation in sows according to claim 1, characterized in that: In step (1), the composition of the first complex enzyme is hemicellulase, cellulase, and pectinase, and the dosages are 1%, 2%, and 3% of the mass of the primary wet powder respectively.

4. The preparation method of a feed additive for reducing sows' constipation according to claim 1, characterized in that: In step (1), based on the mass of the enzymatically hydrolyzed primary wet powder, the addition amount of Saccharomyces cerevisiae liquid is 1% (v / w), the addition amount of Clostridium butyricum liquid is 1% (v / w), and the addition amount of Lactobacillus plantarum liquid is 1% (v / w).

5. The preparation method of a feed additive for reducing constipation in sows according to claim 1, characterized in that: In step (2), the mass ratio of konjac powder, lotus root powder, and pumpkin seed powder is 3 - 7:1 - 5:0.1 - 2; The dosages of ferrous fumarate and sterilized water are 0.01% and 250% of the mass of the mixed medicinal powder respectively; Based on the mass of the polysaccharide complex iron liquid, the addition amount of glucose is 1% (w / w), and the addition amount of Clostridium butyricum liquid is 1% (v / w).

6. The preparation method of a feed additive for reducing constipation in sows according to claim 1, characterized in that: In step (3), the dosages of citric acid monohydrate and sterilized water are 10% and 250% of the mass of the polysaccharide complex iron liquid fermentation material respectively; The dosage of the lactic acid powder is 25% of the mass of the composite iron fermentation broth treated with acid once.

7. The preparation method of a feed additive for reducing sows' constipation according to claim 1, characterized in that: In step (4), the mixing mass ratio of the tea powder and the kiwifruit powder is 7-11:0.1-2; The dosages of cellulase, hemicellulase and sterilized water are 1%, 4% and 70% of the total mass of the tea powder and the kiwifruit powder respectively; Based on the mass of the functional powder enzymolysis material, the addition amount of glucose is 1% (w / w), the addition amount of peptone is 1% (w / w), the addition amount of the Saccharomyces cerevisiae liquid is 1% (v / w), and the addition amount of the Lactobacillus plantarum liquid is 1% (v / w).

8. The preparation method of a feed additive for reducing constipation in sows according to claim 1, characterized in that: In step (5), the mass ratio of the enzymolysis powder fermentation material, the composite iron fermentation broth treated with acid twice and the functional powder fermentation material is 4-6:0.1-2:2-4; The fermentation temperature is 30-35 °C and the fermentation time is 20-28 h.

9. A feed additive prepared by the preparation method of the feed additive according to any one of claims 1-8.

10. An application of the feed additive according to claim 9 in reducing constipation in sows.

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