Functional compound feed for sows in perinatal period as well as preparation method and application of functional compound feed

Abstract

The invention provides functional compound feed for sows in the perinatal period as well as a preparation method and application of the functional compound feed, and relates to the technical field of livestock breeding. The functional compound feed for the sows in the perinatal period is prepared from the following raw materials in parts by mass: 55 to 60 parts of corn, 13 to 15 parts of soybean meal, 1 to 5 parts of fish meal, 6 to 8 parts of extruded soybeans, 2 to 8 parts of barley bran, 0.5 to 1.5 parts of soybean oil, 0.5 to 1.5 parts of glucose, 2 to 8 parts of wheat bran, 1 to 1.5 parts of dicalcium phosphate, 0.5 to 1.5 parts of calcium carbonate, 0.2 to 0.6 part of table salt, 0.05 to 0.2 part of baking soda, 0.05 to 0.2 part of 50 percent choline chloride and 2 to 5 parts of premix. 0.1 to 0.5 part of a composite additive; the compound additive is prepared from the following raw materials in parts by mass: 0.5 to 1 part of ligustrazine, 1 to 5 parts of aloe-emodin glycoside, 0.5 to 2 parts of magnolol and 3 to 5 parts of probiotic microcapsules. Aiming at the perinatal syndrome of the sows, by adjusting the composition and proportion of the raw materials of the compound feed and the preparation method of important raw materials, many problems caused by the perinatal syndrome of the sows are solved, and the compound feed has a good industrial application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of animal husbandry, and in particular to a functional compound feed for sows during the peripartum period, and a preparation method and application thereof. Background Art

[0002] Sows are the core of modern pig production, and their reproductive efficiency directly impacts the economic profitability of pig farms. As a fundamental component of pig production, sow reproductive performance is closely linked to the efficiency of the entire production system. Gestation occupies a significant portion of the sow's reproductive cycle, making feeding and management during gestation crucial for reproductive performance.

[0003] The periparturient period, a critical transition from pregnancy to lactation, is particularly important for the reproductive performance of high-producing sows. There are differing opinions regarding the definition of the periparturient period: narrowly defined as the week before and after farrowing, while the seventh edition of the Merck Veterinary Manual defines it as a longer period, from two weeks before to six weeks after farrowing. During this period, sows undergo significant physiological and metabolic changes, triggered by multiple factors including hormonal fluctuations and environmental stressors. These changes are primarily manifested in metabolic syndrome features such as systemic low-grade inflammation, progressive oxidative stress, and peripartum insulin resistance.

[0004] From a physiological perspective, sows experience a pre-partum inflammatory response during late pregnancy, leading to the accumulation of macrophages in the placenta and the secretion of large amounts of interleukin-6 (IL-6). Meanwhile, levels of the anti-inflammatory factor interleukin-10 (IL-10) fluctuate dynamically throughout pregnancy.

[0005] Recent studies have revealed that peripartum metabolic syndrome in sows may be associated with impaired intestinal barrier function and endotoxin accumulation caused by intestinal flora imbalance. Based on this finding, developing targeted breeding technologies to alleviate peripartum metabolic syndrome is of great significance for improving sow health and reproductive efficiency. Summary of the Invention

[0006] In view of this, the present invention provides a functional compound feed for sows during the peripartum period, as well as a preparation method and application thereof. Aiming at peripartum syndrome, the present invention solves many problems caused by peripartum syndrome in sows by adjusting the raw material composition and ratio of the compound feed and the preparation method of important raw materials, and has good prospects for industrial application.

[0007] The first aspect of the present invention is to provide a functional compound feed for sows during the peripartum period, which specifically comprises the following raw materials in parts by weight: 55 - 60 parts of corn, 13 - 15 parts of soybean meal, 1 - 5 parts of fish meal, 6 - 8 parts of extruded soybean, 2 - 8 parts of barley bran, 0.5 - 1.5 parts of soybean oil, 0.5 - 1.5 parts of glucose, 2 - 8 parts of wheat bran, 1 - 1.5 parts of calcium hydrogen phosphate, 0.5 - 1.5 parts of calcium carbonate, 0.2 - 0.6 parts of salt, 0.05 - 0.2 parts of baking soda, 0.05 - 0.2 parts of 50% choline chloride, 2 - 5 parts of premix, 0.1 - 0.5 parts of compound additive.

[0008] Preferably, the functional compound feed for sows during perinatal period comprises raw materials in the following parts by mass: 58 parts of corn, 14.5 parts of soybean meal, 2 parts of fish meal, 5 parts of extruded soybean, 5.5 parts of barley bran, 1 part of soybean oil, 1 part of glucose, 5.5 parts of wheat bran, 1.2 parts of calcium hydrogen phosphate, 1.1 parts of calcium carbonate, 0.4 parts of salt, 0.15 parts of baking soda, 0.15 parts of 50% choline chloride, 3 parts of premix, 0.3 parts of compound additive.

[0009] Preferably, the protein content of the soybean meal is ≥46%, and the protein content of the fish meal is ≥68%.

[0010] Preferably, the premix comprises raw materials in the following parts by mass: 0.5 - 1.5 parts of vitamin A, 0.2 - 0.8 parts of vitamin D3, 0.5 - 1.5 parts of vitamin E, 0.1 - 0.3 parts of vitamin B1, 0.1 - 0.5 parts of vitamin B2, 0.1 - 0.3 parts of vitamin B6, vitamin B 12 0.005 - 0.015 parts, 0.05 - 0.15 parts of folic acid, 0.05 - 0.15 parts of pantothenic acid, 0.01 - 0.03 parts of biotin, 2 - 8 parts of iron, 0.5 - 1.5 parts of copper, 1 - 5 parts of zinc, 1 - 3 parts of manganese, 0.05 - 0.15 parts of iodine, 0.01 - 0.03 parts of selenium, 3 - 8 parts of lysine, 1 - 5 parts of methionine, 1 - 3 parts of threonine, 0.5 - 1.5 parts of tryptophan, 5 - 7 parts of corn flour.

[0011] More preferably, the premix comprises raw materials in the following parts by mass: 1 part of vitamin A, 0.5 part of vitamin D3, 1 part of vitamin E, 0.2 part of vitamin B1, 0.3 part of vitamin B2, 0.2 part of vitamin B6, vitamin B 12 0.01 part, 0.1 part of folic acid, 0.5 part of pantothenic acid, 0.02 part of biotin, 5 parts of iron, 1 part of copper, 3 parts of zinc, 2 parts of manganese, 0.1 part of iodine, 0.02 part of selenium, 5 parts of lysine, 3 parts of methionine, 2 parts of threonine, 1 part of tryptophan, 6 parts of corn flour.

[0012] Preferably, the compound additive comprises raw materials in the following parts by mass: 0.5 - 1 part of ligustrazine, 1 - 5 parts of aloe-emodin glycoside, 0.5 - 2 parts of magnolol, 3 - 5 parts of probiotic microcapsules.

[0013] More preferably, the composite additive comprises raw materials in the following parts by mass: 0.7 part of ligustrazine, 3 parts of aloe-emodin glycoside, 1.2 parts of magnolol, 4 parts of probiotic microcapsules.

[0014] Preferably, the preparation method of the composite additive is as follows: S2-1: Dissolve sodium alginate, gelatin, glycerol, magnesium chloride or sodium sulfate in deionized water in proportion, and stir until completely dissolved to obtain a microcapsule solution; S2-2: Add the bacterial suspension into the microcapsule solution, mix evenly and then drop it into the calcium chloride solution, and stir for solidification; S2-3: Wash the microcapsules with sterile normal saline or phosphate buffer solution to remove the residual curing agent, and dry to obtain probiotic microcapsules; S2-4: Mix ligustrazine, aloe-emodin glycoside, magnolol with the probiotic microcapsules to obtain the composite additive.

[0015] Preferably, in S2-1, the concentration of sodium alginate in the microcapsule solution is 2 wt.% - 2.5 wt.%, the dosage of gelatin is 10 - 15% of the mass of sodium alginate, the dosage of glycerol is 10% - 20% of the mass of sodium alginate, and the dosage of magnesium chloride or sodium sulfate is 1% - 3% of the mass of sodium alginate. More preferably, the dosage of magnesium chloride is 1% - 2% of the mass of sodium alginate, and the dosage of sodium sulfate is 1% - 3% of the mass of sodium alginate.

[0016] Preferably, in S2-2, the concentration of the bacterial suspension ≥ 1×10 9 CFU / mL, the volume ratio of the bacterial suspension to the microcapsule solution is (0.5 - 1.5):4; the mass concentration of the calcium chloride solution is 0.5% - 1.6%, the dropping speed is 40 - 60 drops / min, the stirring and solidification temperature is 25°C - 40°C, and the stirring and solidification time is 10 - 40 min; the preparation method of the bacterial suspension is: collect Clostridium butyricum and Bacillus subtilis in the logarithmic growth phase, wash and resuspend them with sterile normal saline to a concentration ≥ 1×10 9 CFU / mL, and the volume ratio of the Clostridium butyricum bacterial suspension to the Bacillus subtilis bacterial suspension in the bacterial suspension is 1:(1 - 3).

[0017] Preferably, in S2-3, the drying is vacuum drying or freeze drying; the particle size of the microcapsules is 300 - 2000 μm, and the water content of the probiotic microcapsules < 2%.

[0018] The second aspect of the present invention is to provide a preparation method of the functional compound feed for sows during the perinatal period, which specifically includes the following steps: Corn, soybean meal, extruded soybean, barley bran, and wheat bran are crushed to a particle size of 1-2 mm and then mixed evenly with fish meal, soybean oil, glucose, calcium hydrogen phosphate, calcium carbonate, table salt, baking soda, 50% choline chloride, premix, and compound additive to obtain the functional compound feed for sows during the perinatal period.

[0019] The third aspect of the present invention is to provide an application method of the functional compound feed for sows during the perinatal period.

[0020] Preferably, the functional compound feed for sows during the perinatal period is used in the late pregnancy of sows, from 90 days of pregnancy to one week before parturition. The daily feeding amount is 2.5-3.5 kg, which can be specifically adjusted according to the actual situation of the sows. The daily feeding amount is gradually reduced to 2.0-2.5 kg 5-7 days before parturition and to 1 kg 2-5 days before parturition to reduce the gastrointestinal burden during parturition; no feeding is carried out 8-12 hours before parturition, and the feeding frequency is 3-4 times a day.

[0021] Compared with the prior art, the beneficial technical effects of the present invention are as follows: In the present invention, there is a synergistic effect among tetramethylpyrazine, aloinoside, magnolol, and probiotics (Clostridium butyricum, Bacillus subtilis), which can alleviate the adverse effects brought by the metabolic syndrome of sows during the perinatal period. In order to prevent the mutual influence among tetramethylpyrazine, aloinoside, magnolol, and probiotics, the present invention uses microcapsules to encapsulate the microorganisms and controls the release time of the microorganisms by adjusting the wall material, so as to achieve the best synergistic effect with tetramethylpyrazine, aloinoside, magnolol and improve the survival rate of the microorganisms. The present invention solves many problems brought by the perinatal syndrome of sows and has good industrial application prospects. Description of the Drawings

[0022] The present invention will be further described below with reference to the drawings.

[0023] Figure 1 It is a detection result graph of the total feed intake in Test Example 1; Figure 2 It is a detection result graph of the content of IL-6 in plasma in Test Example 1; Figure 3 It is a detection result graph of the content of IL-10 in plasma in Test Example 1. Specific Embodiments

[0024] Next, in combination with the embodiments of the present invention, the technical solutions of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts fall within the protection scope of the present invention.

[0025] The first aspect of the present invention is to provide a functional compound feed for sows during the perinatal period, which specifically includes the following raw materials in parts by mass: 55 - 60 parts of corn, 13 - 15 parts of soybean meal, 1 - 5 parts of fish meal, 6 - 8 parts of extruded soybean, 2 - 8 parts of barley bran, 0.5 - 1.5 parts of soybean oil, 0.5 - 1.5 parts of glucose, 2 - 8 parts of wheat bran, 1 - 1.5 parts of calcium hydrogen phosphate, 0.5 - 1.5 parts of calcium carbonate, 0.2 - 0.6 parts of table salt, 0.05 - 0.2 parts of baking soda, 0.05 - 0.2 parts of 50% choline chloride, 2 - 5 parts of premix, 0.1 - 0.5 parts of compound additive.

[0026] Preferably, the functional compound feed for sows during the perinatal period of the present invention includes the following raw materials in parts by mass: 58 parts of corn, 14.5 parts of soybean meal, 2 parts of fish meal, 5 parts of extruded soybean, 5.5 parts of barley bran, 1 part of soybean oil, 1 part of glucose, 5.5 parts of wheat bran, 1.2 parts of calcium hydrogen phosphate, 1.1 parts of calcium carbonate, 0.4 parts of table salt, 0.15 parts of baking soda, 0.15 parts of 50% choline chloride, 3 parts of premix, 0.3 parts of compound additive.

[0027] Preferably, the protein content of the soybean meal is ≥ 46%, and the protein content of the fish meal is ≥ 68%.

[0028] The premix of the present invention includes the following raw materials in parts by mass: 0.5 - 1.5 parts of vitamin A, 0.2 - 0.8 parts of vitamin D3, 0.5 - 1.5 parts of vitamin E, 0.1 - 0.3 parts of vitamin B1, 0.1 - 0.5 parts of vitamin B2, 0.1 - 0.3 parts of vitamin B6, vitamin B 12 0.005 - 0.015 parts, 0.05 - 0.15 parts of folic acid, 0.05 - 0.15 parts of pantothenic acid, 0.01 - 0.03 parts of biotin, 2 - 8 parts of iron, 0.5 - 1.5 parts of copper, 1 - 5 parts of zinc, 1 - 3 parts of manganese, 0.05 - 0.15 parts of iodine, 0.01 - 0.03 parts of selenium, 3 - 8 parts of lysine, 1 - 5 parts of methionine, 1 - 3 parts of threonine, 0.5 - 1.5 parts of tryptophan, 5 - 7 parts of corn flour.

[0029] Preferably, the premix includes the following raw materials in parts by mass: 1 part of vitamin A, 0.5 part of vitamin D3, 1 part of vitamin E, 0.2 part of vitamin B1, 0.3 part of vitamin B2, 0.2 part of vitamin B6, vitamin B 12 0.01 part, 0.1 part of folic acid, 0.5 part of pantothenic acid, 0.02 part of biotin, 5 parts of iron, 1 part of copper, 3 parts of zinc, 2 parts of manganese, 0.1 part of iodine, 0.02 part of selenium, 5 parts of lysine, 3 parts of methionine, 2 parts of threonine, 1 part of tryptophan, and 6 parts of corn flour.

[0030] The preparation method of the premix described in the present invention is as follows: S1-1. Raw material pretreatment: Dry the corn flour and sieve it; S1-2: Gradual mixing: ① Primary premixing: Take 10% of the corn flour and mix it with the antioxidant for 1-2 min, then premix vitamin B 12 , biotin, selenium and another 10% of the corn flour and add them, mix for 2-3 min, then premix vitamin A, vitamin D3, vitamin E and another 10% of the corn flour and add them, mix for 3-5 min to obtain the primary mixture; ② Secondary premixing: Add 30% of the corn flour to the primary mixture, synchronously add lysine and methionine, mix for 3-5 min, then add the minerals of iron, zinc and manganese in batches, with an interval of 1-2 min between each batch to obtain the secondary mixture; ③ Tertiary premixing: Add the remaining corn flour and 50% choline chloride to the secondary mixture, mix for 8-10 min, then add vitamin B1, B2, B6 and mix for 3-5 min to obtain the premix.

[0031] Preferably in the present invention, in the S1-1 raw material pretreatment, the water content of the corn flour after drying is ≤5%, and the sieve mesh specification is 60-80 meshes. By pretreating the corn flour, impurities therein are removed, and its adsorption capacity as a carrier is enhanced; in the S1-2 gradual mixing, the antioxidant for primary premixing is ethoxyquinoline, and the dosage of the antioxidant is 0.01% of the total mass of the corn flour; the mixing temperature for secondary premixing is ≤28°C, and the humidity is ≤50% to prevent the amino acids from absorbing moisture, and ensure that copper and vitamin B1 are added separately to ensure that there is a carrier (corn flour) layer interval between the two; the rotation speed during the gradual mixing process is 20-30 rpm.

[0032] The compound additive described in the present invention comprises the following raw materials in parts by mass: 0.5-1 part of tetramethylpyrazine, 1-5 parts of aloinoside, 0.5-2 parts of magnolol, 3-5 parts of probiotic microcapsules.

[0033] Preferably in the present invention, the compound additive comprises the following raw materials in parts by mass: 0.7 parts of ligustrazine, 3 parts of aloe-emodin glycoside, 1.2 parts of magnolol, and 4 parts of probiotic microcapsules.

[0034] The preparation method of the composite additive of the present invention is as follows: S2-1. Dissolve sodium alginate, gelatin, glycerol, magnesium chloride or sodium sulfate in deionized water according to the ratio, and stir until completely dissolved to obtain a microcapsule solution; S2-2. Add the bacterial suspension into the microcapsule solution, mix evenly and then drop it into the calcium chloride solution, and stir for solidification; S2-3. Wash the microcapsules with sterile normal saline or phosphate buffer solution to remove the residual curing agent, and dry to obtain probiotic microcapsules; S2-4. Mix ligustrazine, aloe-emodin glycoside, magnolol and probiotic microcapsules to obtain a composite additive.

[0035] Preferably in the present invention, in S2-1, the concentration of sodium alginate in the microcapsule solution is 2 wt.% - 2.5 wt.%, the dosage of gelatin is 10 - 15% of the mass of sodium alginate, the dosage of glycerol is 10% - 20% of the mass of sodium alginate, and the dosage of magnesium chloride or sodium sulfate is 1% - 3% of the mass of sodium alginate. Preferably, the dosage of magnesium chloride is 1% - 2% of the mass of sodium alginate, and the dosage of sodium sulfate is 1% - 3% of the mass of sodium alginate; in S2-2, the concentration of the bacterial suspension ≥ 1×10 9 CFU / mL, the volume ratio of the bacterial suspension to the microcapsule solution is (0.5 - 1.5):4; the mass concentration of the calcium chloride solution is 0.5% - 1.6%, the dropping speed is 40 - 60 drops / min, the stirring and solidifying temperature is 25°C - 40°C, and the stirring and solidifying time is 10 - 40 min; the preparation method of the bacterial suspension is: collect Clostridium butyricum and Bacillus subtilis in the logarithmic growth phase, wash and resuspend them with sterile normal saline to a concentration ≥ 1×10 9 CFU / mL, and the volume ratio of the Clostridium butyricum suspension to the Bacillus subtilis suspension in the bacterial suspension is 1:(1 - 3); in S2-3, drying is vacuum drying or freeze drying; the particle size of the microcapsules is 300 - 2000 μm, and the water content of the probiotic microcapsules < 2%.

[0036] The second aspect of the present invention is to provide a preparation method of the functional composite feed for the perinatal period of sows, which specifically includes the following steps: Crush corn, soybean meal, extruded soybean, barley bran, and wheat bran to a particle size of 1 - 2 mm, and then mix them evenly with fish meal, soybean oil, glucose, calcium hydrogen phosphate, calcium carbonate, table salt, baking soda, 50% choline chloride, premix, and composite additive to obtain the functional composite feed for the perinatal period of sows.

[0037] The third aspect of the present invention is to provide an application method of the functional compound feed for sows during the perinatal period.

[0038] The functional compound feed for sows during the perinatal period of the present invention is used in the late pregnancy of sows, from 90 days of pregnancy to one week before parturition. The daily feeding amount is 2.5 - 3.5 kg, and it can be specifically adjusted according to the actual situation of the sows. The daily feeding amount is gradually reduced to 2.0 - 2.5 kg 5 - 7 days before parturition, and gradually reduced to 1 kg 2 - 5 days before parturition, so as to reduce the gastrointestinal burden during parturition; no feeding is carried out 8 - 12 hours before parturition, and the feeding frequency is 3 - 4 times a day.

[0039] To further illustrate the present invention, the following examples are used for detailed description. All raw materials used in the following examples of the present invention are commercially available.

[0040] Unless otherwise specified, all experiments are repeated 3 times. ANOVA (Analysis of Variance) and Duncan multiple comparison analysis are performed using SPSS 21.0, and the results are expressed as mean ± standard deviation. A significant difference is indicated when P < 0.05. Origin2021 software is used for graphing.

[0041] The preparation method of the bacterial suspension involved in the examples and comparative examples of the present invention is as follows: Collect Clostridium butyricum and Bacillus subtilis in the logarithmic growth phase, wash with sterile physiological saline and resuspend to a concentration ≥ 1×10 9 CFU / mL.

[0042] Example 1 A functional compound feed for sows during the perinatal period, which is composed of the following raw materials in parts by mass: 58 parts of corn, 14.5 parts of soybean meal (protein content 53%), 2 parts of fish meal (protein content 72%), 5 parts of expanded soybean, 5.5 parts of barley bran, 1 part of soybean oil, 1 part of glucose, 5.5 parts of wheat bran, 1.2 parts of calcium hydrogen phosphate, 1.1 parts of calcium carbonate, 0.4 part of table salt, 0.15 part of sodium bicarbonate, 0.15 part of 50% choline chloride, 3 parts of premix, 0.3 part of compound additive; The premix is composed of the following raw materials in parts by mass: 1 part of vitamin A, 0.5 part of vitamin D3, 1 part of vitamin E, 0.2 part of vitamin B1, 0.3 part of vitamin B2, 0.2 part of vitamin B6, vitamin B 12 0.01 part, 0.1 part of folic acid, 0.5 part of pantothenic acid, 0.02 part of biotin, 5 parts of iron, 1 part of copper, 3 parts of zinc, 2 parts of manganese, 0.1 part of iodine, 0.02 part of selenium, 5 parts of lysine, 3 parts of methionine, 2 parts of threonine, 1 part of tryptophan, 6 parts of corn flour; The preparation method of the premix is as follows: Dry the corn flour until the water content ≤ 5%, and sieve it through a 60-mesh sieve; Primary premixing: Take 10% of the corn flour and mix it with 0.01% of ethoxyquinoline based on the total amount of corn flour for 1 min, then add the premix of vitamin B 12 , biotin, selenium and another 10% of the corn flour, mix for 2 min, then add the premix of vitamin A, vitamin D3, vitamin E and another 10% of the corn flour, mix for 3 min to obtain the primary mixture; Secondary premixing: Add 30% of the corn flour to the primary mixture, synchronously add lysine and methionine, and mix for 3 min under the conditions of temperature 25°C ± 2°C and humidity 45% ± 5%, then add the minerals of iron, zinc and manganese in batches, with an interval of 1 min between each batch to obtain the secondary mixture; Tertiary premixing: Add the remaining corn flour and 50% choline chloride to the secondary mixture, mix for 8 min, then add vitamin B1, B2, B6 and mix for 3 min to obtain the premix. The rotation speed of each mixing step above is 25 rpm ± 2 rpm; The compound additive is composed of the following raw materials in parts by mass: 0.7 part of tetramethylpyrazine, 3 parts of aloe-emodin glycoside, 1.2 parts of magnolol, 4 parts of probiotic microcapsules; The preparation method of the compound additive is as follows: Dissolve sodium alginate, gelatin, glycerol, and magnesium chloride in deionized water according to the ratio, and stir until completely dissolved to obtain a microcapsule solution. The concentration of sodium alginate is 2.5 wt.%, the dosage of gelatin is 12% of the mass of sodium alginate, the dosage of glycerol is 15% of the mass of sodium alginate, and the dosage of sodium sulfate is 2% of the mass of sodium alginate; Mix the Clostridium butyricum suspension with a concentration of 2.3×10 9 CFU / mL and the Bacillus subtilis suspension with a concentration of 1.8×10 9 CFU / mL according to a volume ratio of 1:2.5 and add them to the microcapsule solution. The volume ratio of the suspension and the microcapsule solution is 1.2:4. After mixing evenly, drop it into a calcium chloride solution with a mass concentration of 1.4% at a speed of 50 drops / min, and stir and solidify at 30°C with a rotation speed of 160 rpm for 30 min; Wash the microcapsules with sterile physiological saline to remove the residual calcium chloride solution, and freeze-dry to obtain probiotic microcapsules with a water content < 2% and a particle size of 300 - 2000 μm; Then mix tetramethylpyrazine, aloe-emodin glycoside, magnolol with the probiotic microcapsules to obtain the compound additive.

[0043] Comparative Example 1 The difference from Example 1 is that the compound additive is not used.

[0044] Comparative Example 2 The difference from Example 1 is that the compound additive does not contain probiotic microcapsules.

[0045] Comparative Example 3 The difference from Example 1 is that: the compound additive does not contain tetramethylpyrazine, aloe-emodin glycoside, and magnolol.

[0046] Comparative Example 4 The difference from Example 1 is that: the wall material of the microcapsules is different: 4-1 Dissolve sodium alginate in deionized water and stir until completely dissolved to obtain a microcapsule solution. The remaining steps are the same as in Example 1; 4-2 Dissolve sodium alginate and gelatin in deionized water and stir until completely dissolved to obtain a microcapsule solution. The remaining steps are the same as in Example 1; 4-3 Dissolve sodium alginate, gelatin, and magnesium chloride in deionized water and stir until completely dissolved to obtain a microcapsule solution. The remaining steps are the same as in Example 1; 4-4 Dissolve sodium alginate, gelatin, and glycerol in deionized water and stir until completely dissolved to obtain a microcapsule solution. The remaining steps are the same as in Example 1;

[0047] Comparative Example 5 The difference from Example 1 is that: the probiotics are added in the form of a bacterial solution, specifically: Mix a Clostridium butyricum bacterial suspension with a concentration of 2.3×10 9 CFU / mL and a Bacillus subtilis bacterial suspension with a concentration of 1.8×10 9 CFU / mL in a volume ratio of 1:2.5, and then add tetramethylpyrazine, aloe-emodin glycoside, and magnolol to obtain a compound additive.

[0048] Test Example 1 Select 90 pregnant sows in good health, with the same genetic background and parity (all the second parity), and divide them into 6 experimental groups by completely random design, with 15 sows in each group. The experimental period starts from 90 days of pregnancy and lasts until the end of parturition. The feeding method is single-stall feeding in a gestation pen with the following environmental parameters set: temperature 18°C - 22°C, humidity 60% - 70%, ensuring air circulation and keeping the pen dry. From 90 days of pregnancy to one week before parturition, the daily feeding amount is 3.0 kg, and it is gradually reduced to 2.0 kg from 5 - 7 days before parturition and to 1 kg from 2 - 5 days before parturition. No feeding is carried out 10 hours before parturition. The feeding frequency is 3 times a day, and the feeding time of each group is strictly synchronized. Ensure sufficient and clean drinking water, and other feeding management follows the pig farm breeding procedures. The feed used is the feed prepared in Example 1 and Comparative Examples 1 - 4. Plasma collection is carried out by conventional sampling methods.

[0049] 1. Total feed intake (90 - 110 d) The total feed intake of sows in each group during the late pregnancy period is asFigure 1 As shown by Figure 1 Combined with data analysis, in terms of feed intake in the late pregnancy period, there was no significant difference in the total feed intake of sows in each group during the late pregnancy period (P>0.05). It can be seen that the use of the compound additive does not affect the normal feeding behavior of sows, indicating that the additive has good palatability and safety.

[0050] 2. Inflammatory conditions The contents of IL-6 and IL-10 in the plasma samples of sows were measured using an ELISA kit, and the results are as Figure 2 , Figure 3 , Table 1, and Table 2 shown.

[0051] Table 1 P values between groups for IL-6 detection results

[0052] Table 2 P values between groups for IL-10 detection results

[0053] From Figure 2 , Figure 3 , Table 1, and Table 2, it can be known that the functional compound feed for sows during the perinatal period of the present invention can alleviate the systemic inflammatory response of sows during the perinatal period.

[0054] Comparative Example 6 The difference from Example 1 is that: 6-1: Clostridium butyricum was not used, and an equal amount (effective viable count) of Bacillus subtilis was used instead; 6-2: An equal amount (effective viable count) of Bifidobacterium was used instead of Clostridium butyricum.

[0055] Comparative Example 7: The difference from Example 1 is that: Magnolol was not used, and the missing part was supplemented with tetramethylpyrazine and aloe-emodin glycoside in proportion.

[0056] Test Example 2 Limited by the source of experimental animals, the synergistic effect between probiotics and tetramethylpyrazine, aloe-emodin glycoside, and magnolol was tested in the second batch. The selection, breeding, and feeding methods of experimental animals were the same as those in Test Example 1.

[0057] The selection of experimental animals was the same as that in Example 1.

[0058] 1. Total feed intake (90 - 110 d) The detection method was the same as that in Test Example 1. After detection and data analysis, it was found that there was no significant difference in the feed intake of sows in the late pregnancy among Comparative Example 6, Comparative Example 7 and Example 1 (parallel group) (P>0.05). It can be seen that the use of Clostridium butyricum and magnolol does not affect the normal feeding behavior of sows, indicating that this additive has good palatability and safety.

[0059] 2. Inflammatory conditions The detection method was the same as that in Test Example 1, and the results are shown in Table 3 and Table 4.

[0060] Table 3 Detection results of IL-6

[0061] Note: Different lowercase letters in the same column in the table indicate significant differences between the two (P<0.05).

[0062] Table 4 Detection results of IL-10

[0063] Note: Different lowercase letters in the same column in the table indicate significant differences between the two (P<0.05).

[0064] It can be seen from Table 3 and Table 4 that the use of Clostridium butyricum and magnolol can alleviate the systemic inflammatory response of sows during the perinatal period.

[0065] Example 2 A functional compound feed for sows during the perinatal period, which is composed of the following raw materials in parts by mass: 55 parts of corn, 13 parts of soybean meal (protein content 53%), 1 part of fish meal (protein content 72%), 6 parts of expanded soybean, 2 parts of barley bran, 0.5 part of soybean oil, 0.5 part of glucose, 2 parts of wheat bran, 1 part of calcium hydrogen phosphate, 0.5 part of calcium carbonate, 0.2 part of table salt, 0.15 part of baking soda, 0.05 part of 50% choline chloride, 2 parts of premix, 0.1 part of compound additive; The rest is the same as in Example 1.

[0066] Example 3 A functional compound feed for sows during the perinatal period, which is composed of the following raw materials in parts by mass: 60 parts of corn, 15 parts of soybean meal (protein content 53%), 5 parts of fish meal (protein content 72%), 8 parts of expanded soybean, 8 parts of barley bran, 15 parts of soybean oil, 1.5 parts of glucose, 8 parts of wheat bran, 1.5 part of calcium hydrogen phosphate, 1.5 parts of calcium carbonate, 0.6 part of table salt, 0.2 part of baking soda, 0.2 part of 50% choline chloride, 5 parts of premix, 0.5 part of compound additive; The rest is the same as in Example 1.

[0067] Example 4: A functional compound feed for sows during the perinatal period, wherein the premix is composed of the following raw materials in parts by mass: Vitamin A 0.5 part, vitamin D3 0.2 part, vitamin E 0.5 part, vitamin B1 0.1 part, vitamin B2 0.1 part, vitamin B6 0.1 part, vitamin B 12 0.005 part, folic acid 0.05 part, pantothenic acid 0.05 part, biotin 0.01 part, iron 2 parts, copper 0.5 part, zinc 1 part, manganese 1 part, iodine 0.05 part, selenium 0.01 part, lysine 3 parts, methionine 1 part, threonine 1 part, tryptophan 0.5 part, corn flour 5 parts; The rest is the same as in Example 1.

[0068] Example 5: A functional compound feed for sows during the perinatal period, wherein the premix is composed of the following raw materials in parts by mass: Vitamin A 1.5 parts, vitamin D3 0.8 part, vitamin E 1.5 part, vitamin B1 0.3 part, vitamin B2 0.5 part, vitamin B6 0.3 part, vitamin B 12 0.015 part, folic acid 0.15 part, pantothenic acid 0.15 part, biotin 0.03 part, iron 8 parts, copper 1.5 part, zinc 5 part, manganese 3 parts, iodine 0.15 part, selenium 0.03 part, lysine 8 parts, methionine 5 parts, threonine 3 parts, tryptophan 1.5 parts, corn flour 7 parts; The rest is the same as in Example 1.

[0069] Example 6: A functional compound feed for sows during the perinatal period, wherein the compound additive is composed of the following raw materials in parts by mass: Ligustrazine 0.5 part, aloe-emodin glycoside 1 part, magnolol 0.5 part, probiotic microcapsule 3 parts.

[0070] Example 7: A functional compound feed for sows during the perinatal period, wherein the compound additive is composed of the following raw materials in parts by mass: Ligustrazine 1 part, aloe-emodin glycoside 5 parts, magnolol 2 parts, probiotic microcapsule 5 parts.

[0071] After testing, there is no significant difference in the effects of the feeds in Examples 2 - 7 and Example 1.

[0072] The above-described embodiments only represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the appended claims.

Claims

1. A functional compound feed for sows during the perinatal period, characterized in that, It comprises raw materials in the following parts by mass: 55 - 60 parts of corn, 13 - 15 parts of soybean meal, 1 - 5 parts of fish meal, 6 - 8 parts of extruded soybean, 2 - 8 parts of barley bran, 0.5 - 1.5 parts of soybean oil, 0.5 - 1.5 parts of glucose, 2 - 8 parts of wheat bran, 1 - 1.5 parts of calcium hydrogen phosphate, 0.5 - 1.5 parts of calcium carbonate, 0.2 - 0.6 parts of table salt, 0.05 - 0.2 parts of sodium bicarbonate, 0.05 - 0.2 parts of 50% choline chloride, 2 - 5 parts of premix, 0.1 - 0.5 parts of compound additive; The compound additive comprises raw materials in the following parts by mass: 0.5 - 1 part of tetramethylpyrazine, 1 - 5 parts of aloinoside, 0.5 - 2 parts of magnolol, 3 - 5 parts of probiotic microcapsules.

2. The functional compound feed for perinatal sows according to claim 1, wherein, The functional compound feed for sows during perinatal period comprises raw materials in the following parts by mass: 58 parts of corn, 14.5 parts of soybean meal, 2 parts of fish meal, 5 parts of extruded soybean, 5.5 parts of barley bran, 1 part of soybean oil, 1 part of glucose, 5.5 parts of wheat bran, 1.2 parts of calcium hydrogen phosphate, 1.1 parts of calcium carbonate, 0.4 parts of table salt, 0.15 parts of sodium bicarbonate, 0.15 parts of 50% choline chloride, 3 parts of premix, 0.3 parts of compound additive.

3. The functional compound feed for perinatal sows according to claim 1 or 2, characterized in that, The protein content of the soybean meal is ≥46%, and the protein content of the fish meal is ≥68%.

4. The functional compound feed for perinatal sows according to claim 1 or 2, characterized in that The premix comprises raw materials in the following parts by mass: Vitamin A 0.5 - 1.5 parts, Vitamin D3 0.2 - 0.8 parts, Vitamin E 0.5 - 1.5 parts, Vitamin B1 0.1 - 0.3 parts, Vitamin B2 0.1 - 0.5 parts, Vitamin B6 0.1 - 0.3 parts, Vitamin B 12 0.005 - 0.015 parts, folic acid 0.05 - 0.15 parts, pantothenic acid 0.05 - 0.15 parts, biotin 0.01 - 0.03 parts, iron 2 - 8 parts, copper 0.5 - 1.5 parts, zinc 1 - 5 parts, manganese 1 - 3 parts, iodine 0.05 - 0.15 parts, selenium 0.01 - 0.03 parts, lysine 3 - 8 parts, methionine 1 - 5 parts, threonine 1 - 3 parts, tryptophan 0.5 - 1.5 parts, corn flour 5 - 7 parts.

5. The functional compound feed for perinatal sows according to claim 4, characterized in that The premix comprises raw materials in the following parts by mass: 1 part of vitamin A, 0.5 part of vitamin D3, 1 part of vitamin E, 0.2 part of vitamin B1, 0.3 part of vitamin B2, 0.2 part of vitamin B6, vitamin B 12 0.01 part, 0.1 part of folic acid, 0.5 part of pantothenic acid, 0.02 part of biotin, 5 parts of iron, 1 part of copper, 3 parts of zinc, 2 parts of manganese, 0.1 part of iodine, 0.02 part of selenium, 5 parts of lysine, 3 parts of methionine, 2 parts of threonine, 1 part of tryptophan, 6 parts of corn flour.

6. The functional compound feed for perinatal sows according to claim 1 or 2, characterized in that, The compound additive comprises raw materials in the following parts by mass: 0.7 part of tetramethylpyrazine, 3 parts of aloinoside, 1.2 parts of magnolol, 4 parts of probiotic microcapsules.

7. A functional compound feed for sows during the perinatal period, characterized in that, according to claim 1 or 2, The preparation method of the compound additive is as follows: Dissolve the microcapsule wall material in deionized water and stir until completely dissolved to obtain a microcapsule solution; add the bacterial suspension into the microcapsule solution, mix evenly and then drop it into calcium chloride solution, stir and solidify; wash the microcapsules with sterile normal saline or phosphate buffer solution to remove the residual curing agent, and dry to obtain probiotic microcapsules; mix tetramethylpyrazine, aloinoside, magnolol with the probiotic microcapsules to obtain the compound additive.

8. The functional compound feed for perinatal sows according to claim 7, wherein The microcapsule wall material comprises sodium alginate, gelatin, glycerol, magnesium chloride or sodium sulfate.

9. The preparation method of the functional compound feed for sows during the perinatal period according to any one of claims 1-8, characterized in that, It comprises the following steps: Crush corn, soybean meal, extruded soybean, barley bran, wheat bran to a particle size of 1 - 2 mm, and then mix them evenly with fish meal, soybean oil, glucose, calcium hydrogen phosphate, calcium carbonate, table salt, sodium bicarbonate, 50% choline chloride, premix, compound additive to obtain the functional compound feed for sows during perinatal period.

10. The application method of the functional compound feed for sows during the perinatal period according to any one of claims 1-8, characterized in that, The functional compound feed for sows during perinatal period is used for the late pregnancy of sows.

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