Composition with effects of resisting fatigue and reducing uric acid and health food containing composition

Abstract

The composition is prepared from the following components in parts by weight: 0.1 to 50 parts of chicken protein peptide powder, 0.1 to 12 parts of marine fish oligopeptide powder, 1 to 11 parts of herba houttuyniae powder, 4 to 10 parts of cichorium intybus powder, 2 to 10 parts of poria cocos powder, 2 to 10 parts of sophora flower bud powder, 2 to 10 parts of fructus amomi powder, 2 to 6 parts of opuntia ficus-indica juice powder and 1 to 5 parts of sour cherry fruit powder. The composition disclosed by the invention shows remarkable technical effects in the aspects of reducing uric acid and resisting fatigue through the synergistic effect of various components, and a safe and effective nutritional supplement and health improvement scheme is provided for people suffering from hyperuricemia and fatigable people.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and particularly relates to a composition with anti-fatigue and uric acid-lowering effects and a health food containing the same. Background Art

[0002] With the acceleration of the modern life rhythm and the significant change in the diet structure, the incidence rate of hyperuricemia has been showing an upward trend year by year. Hyperuricemia not only causes gout, leading to severe joint pain, swelling and even deformity, seriously affecting the quality of life of patients, but also is closely related to various chronic diseases such as cardiovascular diseases, diabetes, and chronic kidney diseases, posing a great threat to human health. According to statistics, in some developed countries, the prevalence rate of hyperuricemia has exceeded 20%, and in our country, its prevalence rate is also in the range of 10%-20%, and shows a trend of getting younger.

[0003] At the same time, factors such as long-term work pressure, irregular work and rest, and lack of exercise have made fatigue spread widely among the population. Fatigue not only reduces people's work efficiency and life satisfaction, but long-term accumulation may also cause chronic fatigue syndrome, causing serious damage to physical health. Some studies have shown that about 60%-70% of office workers are in a state of fatigue at different levels for a long time.

[0004] Currently, for the treatment of hyperuricemia, it mainly relies on drugs such as allopurinol and febuxostat. These drugs reduce the blood uric acid level by inhibiting uric acid synthesis or promoting uric acid excretion. However, drug treatment is often accompanied by many side effects. For example, allopurinol may cause severe allergic reactions, and febuxostat may lead to abnormal liver function, etc. In addition, some patients have poor compliance with drug treatment and are difficult to adhere to it for a long time. In terms of anti-fatigue, common methods include ingesting drinks rich in caffeine, using health products, etc. But caffeine can only refresh temporarily, and excessive intake may cause adverse reactions such as palpitations and insomnia; the existing anti-fatigue health products have relatively single ingredients, limited effects, and may cause potential harm to the body if used for a long time.

[0005] Therefore, it has important practical significance to develop a composition that is safe, effective and has dual effects of anti-fatigue and uric acid-lowering. It can not only meet the health needs of the vast number of hyperuricemia patients and fatigued people, but also fill the gap in related products in the market and provide a new choice for improving the public health status. Summary of the Invention

[0006] Therefore, based on the above defects of the prior art, the present invention provides a composition with anti-fatigue and uric acid-lowering effects and a health food containing the composition.

[0007] To solve the above technical problems, the present invention provides the following technical solutions:

[0008] The first aspect of the present invention provides a composition with the effects of anti-fatigue and reducing uric acid, comprising the following components by weight: 0.1-50 parts of chicken protein peptide powder, 0.1-12 parts of marine fish oligopeptide powder, 1-11 parts of houttuynia powder, 4-10 parts of chicory powder, 2-10 parts of poria powder, 2-10 parts of sophora japonica powder, 2-10 parts of amomum villosum powder, 2-6 parts of prickly pear cactus juice powder, and 1-5 parts of tart cherry fruit powder.

[0009] The composition provided by the present invention not only has the effect of reducing uric acid, but also has the function of anti-fatigue. The specific mechanism is described as follows:

[0010] The marine fish oligopeptide powder is rich in various bioactive peptides, which can regulate the activity of key enzymes in purine metabolism in the body, inhibit the activity of xanthine oxidase, and reduce the conversion of xanthine and hypoxanthine, the precursors of uric acid production, into uric acid, thereby reducing the amount of uric acid produced from the source. At the same time, after the chicken protein peptide powder is prepared by a specific process, certain small molecule peptides contained therein can act on the signal pathway related to uric acid synthesis, block or slow down the uric acid production process, and further synergistically reduce uric acid production.

[0011] The chicory powder contains rich components such as chicoric acid and aesculetin, which can increase the excretion ability of the kidneys to uric acid and promote the excretion of uric acid through urine. The poria powder can regulate the water metabolism function of the kidneys, enhance the inhibitory effect of renal tubules on the reabsorption of uric acid, and improve the uric acid excretion efficiency. The two work synergistically to strengthen the body's uric acid excretion mechanism and help reduce the blood uric acid level.

[0012] Flavonoid compounds such as rutin in the sophora japonica powder and volatile oil components in the amomum villosum powder can regulate the internal environment of uric acid metabolism in the body, and by affecting the deposition and dissolution balance of urate crystals in joints and tissues, reduce the formation and accumulation of urate crystals, thereby alleviating symptoms such as joint pain caused by hyperuricemia and maintaining the dynamic balance of the body's uric acid metabolism.

[0013] The houttuynia powder has significant anti-inflammatory activity, and components such as quercitrin and isoquercitrin contained therein can inhibit the release of inflammatory factors and reduce the inflammatory response caused by hyperuricemia. In an inflammatory state, uric acid metabolism is prone to disorder, and the anti-inflammatory effect helps to restore the normal uric acid metabolism environment, assist in reducing the uric acid level, and at the same time relieve the inflammatory symptoms during a gout attack.

[0014] Both the chicken protein peptide powder and the marine fish oligopeptide powder are high-quality sources of small molecule peptides, which can be quickly absorbed by the human body, supply energy to the body, repair damaged muscle tissues, promote muscle protein synthesis, and reduce the generation of muscle fatigue after exercise. At the same time, these peptide substances can provide various essential amino acids for the body, maintain the normal metabolic function of the body, and improve the body's tolerance to fatigue.

[0015] Sour cherry fruit powder is rich in various antioxidant substances such as anthocyanins. Prickly pear cactus juice powder contains a large amount of antioxidants such as polysaccharides and flavonoids. They can scavenge excessive free radicals generated in the body due to fatigue, reduce the oxidative damage of free radicals to cells, protect the integrity of cell membranes, and maintain the normal functions of cells. Thus, it can relieve the fatigue caused by oxidative stress and improve the anti-fatigue ability of the body.

[0016] Components such as poria powder, amomum villosum powder, and sophora japonica powder work synergistically to regulate various metabolic pathways in the body, such as sugar metabolism and lipid metabolism. By improving the utilization efficiency of energy substances in the body, it ensures that various tissues and organs have sufficient energy supply in a fatigued state, enhancing the endurance and anti-fatigue ability of the body. At the same time, they can also regulate the endocrine system, maintain the stability of hormone levels, and further relieve fatigue symptoms.

[0017] In addition, various components in the composition, such as houttuynia cordata powder and poria powder, have immunomodulatory effects and can enhance the body's immunity. When the body's immunity is improved, the resistance to fatigue is also correspondingly enhanced, which helps the body recover from the fatigued state faster and reduces the accumulation of fatigue.

[0018] Furthermore, the mass ratio of the chicken protein peptide powder to the marine fish oligopeptide powder is 2:1 to 5:1.

[0019] If the proportion of chicken protein peptide powder is too high, it may produce more uric acid during metabolism in the body due to more nitrogen-containing substances, which is not conducive to reducing uric acid. While if the proportion of marine fish oligopeptide powder is too high, the comprehensive effect in terms of anti-fatigue may not be ideal enough, because chicken protein peptide powder has its unique advantages in providing comprehensive nutritional support for muscle recovery and so on.

[0020] Furthermore, it includes the following components by weight: 30 - 40 parts of chicken protein peptide powder, 10 - 15 parts of marine fish oligopeptide powder, 2 - 6 parts of houttuynia cordata powder, 5 - 8 parts of chicory powder, 4 - 8 parts of poria powder, 2 - 6 parts of sophora japonica powder, 2 - 6 parts of amomum villosum powder, 3 - 6 parts of prickly pear cactus juice powder, and 3 - 5 parts of sour cherry fruit powder.

[0021] Furthermore, it includes the following components by weight: 36 parts of chicken protein peptide powder, 12 parts of marine fish oligopeptide powder, 4 parts of houttuynia cordata powder, 6 parts of chicory powder, 6 parts of poria powder, 4 parts of sophora japonica powder, 4 parts of amomum villosum powder, 5 parts of prickly pear cactus juice powder, and 4 parts of sour cherry fruit powder.

[0022] Furthermore, the preparation method of the chicken protein peptide powder is:

[0023] Freeze the chicken, crush it, mix it with water, heat the mixture, and then add protease for stepwise enzymatic hydrolysis; after the enzymatic hydrolysis is completed, filter the hydrolysate with an ultrafiltration membrane; then, adjust the pH to acidic, add saccharide substances to the filtrate, and add probiotic bacterial liquid for fermentation; after the obtained fermentation broth is freeze-dried, chicken protein peptide powder is obtained.

[0024] Furthermore, the stepwise enzymatic hydrolysis includes the first-stage enzymatic hydrolysis, the second-stage enzymatic hydrolysis, and the third-stage enzymatic hydrolysis;

[0025] The protease used in the first-stage enzymatic hydrolysis is alkaline protease, the pH value is adjusted to 8-10, the temperature is controlled at 40°C-50°C, and the enzymatic hydrolysis is carried out for 2-3 hours; the protease used in the second-stage enzymatic hydrolysis is trypsin, the pH value is adjusted to 7.5-8.5, the temperature is controlled at 37°C, and the enzymatic hydrolysis is carried out for 2-3 hours; the protease used in the third-stage enzymatic hydrolysis is neutral protease, the pH value is adjusted to 6-7, the temperature is controlled at 40°C-45°C, and the enzymatic hydrolysis is carried out for 1-2 hours.

[0026] Furthermore, during the first-stage enzymatic hydrolysis, the addition amount of alkaline protease is 0.8-1.5% of the weight of the chicken.

[0027] Furthermore, during the second-stage enzymatic hydrolysis, the addition amount of trypsin is 1-2% of the weight of the chicken.

[0028] Furthermore, during the third-stage enzymatic hydrolysis, the addition amount of neutral protease is 0.2-1% of the weight of the chicken.

[0029] Furthermore, the mass ratio of the chicken to water is 1:8-12.

[0030] Furthermore, the cut-off molecular weight of the ultrafiltration membrane is 3000-5000 Da.

[0031] Furthermore, the pH is adjusted to 4.5-6.5.

[0032] Furthermore, the saccharide substances include at least one of glucose, fructooligosaccharide, and galactooligosaccharide, and the addition amount is 2-5% of the weight of the filtrate.

[0033] Furthermore, the probiotic is Lactobacillus acidophilus CICC 20250, and its concentration is (1-6)×10 8 CFU / mL, and the inoculation amount is 1-3%.

[0034] Furthermore, the fermentation temperature is 35-40°C, and the fermentation time is 12-24 hours.

[0035] Furthermore, the temperature of the freeze-drying is -60--80°C, and the time of the freeze-drying is 24-48 h.

[0036] In the second aspect of the present invention, a health food is provided, and the health food contains the composition with the effects of anti-fatigue and reducing uric acid.

[0037] Furthermore, the health food further includes excipients, and the excipients include at least one of fillers, edible flavors, acidulants, sweeteners, and diluents.

[0038] Furthermore, the filler is one or more of fructose, erythritol, isomaltitol, mannitol, starch, and dextrin.

[0039] Furthermore, the edible flavor is one or more of orange flavor, lemon flavor, strawberry flavor, blueberry flavor, apple flavor, and rose flavor.

[0040] Furthermore, the sweetener is one or more of brown sugar, sucralose, stevioside, aspartame, and neotame.

[0041] Furthermore, the acidulant is one or more of citric acid, lactic acid, tartaric acid, fumaric acid, and sodium citrate.

[0042] Furthermore, the diluent is one or more of water, edible vegetable oil, and polyethylene glycol.

[0043] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0044] 1. The composition provided by the present invention shows significant technical effects in both reducing uric acid and anti-fatigue through the synergistic effect of various components, providing a safe and effective nutritional supplement and health improvement solution for people with hyperuricemia and easy fatigue.

[0045] 2. For the composition provided by the present invention, the chicken protein peptide powder is obtained through segmented enzymatic hydrolysis, ultrafiltration, and fermentation. Among them, segmented enzymatic hydrolysis and ultrafiltration are beneficial for obtaining more small-molecule peptides with uric acid-lowering activity, improving the purity of the active peptide segments, and enhancing the effect of reducing uric acid; while fermenting the filtrate, on the one hand, Lactobacillus acidophilus can utilize the small-molecule peptides and amino acids produced by enzymatic hydrolysis and produce specific functional metabolites, such as vitamins and exopolysaccharides. These metabolites have antioxidant and immune regulatory functions, may help relieve fatigue and regulate uric acid metabolism, and can synergistically act with the original components in the enzymatic hydrolysate to further enhance its effects of reducing uric acid and anti-fatigue. On the other hand, the enzymatic hydrolysate may itself have a certain fishy smell or bad odor, and through fermentation, unique flavor substances can be produced, which can transform or remove these bad flavors, improve the flavor and taste of the enzymatic hydrolysate, and make it more acceptable.

[0046] 3. The composition provided by the present invention can be applied to various health foods, making them have the effects of reducing uric acid and anti-fatigue. Detailed implementation manners

[0047] The present invention will be further described below in conjunction with specific embodiments, so that those skilled in the art can better understand the present invention and be able to implement it, but the specific embodiments cited are not intended to limit the present invention.

[0048] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the description of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0049] Unless otherwise specified, the experimental methods used in the following examples and comparative examples are all conventional methods, and the materials, reagents, etc. used can be obtained from commercial sources unless otherwise specified.

[0050] Example 1

[0051] This example provides a composition with the effects of anti-fatigue and reducing uric acid, including the following components by weight: 36 parts of chicken protein peptide powder, 12 parts of marine fish oligopeptide powder, 4 parts of houttuynia powder, 6 parts of chicory powder, 6 parts of poria powder, 4 parts of sophora japonica powder, 4 parts of amomum villosum powder, 5 parts of prickly pear cactus juice powder, and 4 parts of tart cherry fruit powder.

[0052] Among them, the preparation method of the chicken protein peptide powder includes the following steps:

[0053] (1) Freeze the chicken and then crush it, add deionized water in a ratio of 1:10, heat it in a water bath to 45°C, adjust the pH to 9.0, add alkaline protease accounting for 1.2% of the weight of the chicken, and enzymatically hydrolyze for 3 hours under magnetic stirring;

[0054] (2) Adjust the pH of the enzymatic hydrolysate to 8.0, the temperature of the water bath heating is 37°C, add trypsin accounting for 1.5% of the weight of the chicken, and enzymatically hydrolyze for 2.5 hours under magnetic stirring;

[0055] (3) Adjust the pH of the enzymatic hydrolysate to 6.5, the temperature of the water bath heating is 42°C, add neutral protease accounting for 0.8% of the weight of the chicken, and enzymatically hydrolyze for 1.5 hours under magnetic stirring;

[0056] (4) After the enzymatic hydrolysis is completed, filter the enzymatic hydrolysate with an ultrafiltration membrane with a molecular weight cut-off of 3000 Da to obtain a filtrate;

[0057] (5) Adjust the pH of the filtrate to 5.0, add 3 wt% of fructooligosaccharide to the filtrate, and then add probiotic liquid at an inoculation amount of 1 wt%. Ferment at 38 °C for 24 hours to obtain a fermentation broth; wherein, the probiotic is Lactobacillus acidophilus CICC 20250, and its liquid concentration is 5×10 8 CFU / mL;

[0058] (6) Freeze-dry the obtained fermentation broth at -80 °C for 48 hours to obtain chicken protein peptide powder.

[0059] Example 2

[0060] This example provides a composition with anti-fatigue and uric acid-lowering effects, including the following components by weight: 40 parts of chicken protein peptide powder, 15 parts of marine fish oligopeptide powder, 5 parts of houttuynia powder, 8 parts of chicory powder, 5 parts of poria powder, 5 parts of sophora flower powder, 5 parts of amomum powder, 4 parts of prickly pear cactus juice powder, and 3 parts of tart cherry fruit powder.

[0061] Among them, the preparation method of the chicken protein peptide powder includes the following steps:

[0062] (1) Freeze and crush the chicken, add deionized water according to a ratio of 1:8, heat in a water bath to 45 °C, adjust the pH to 9.0, add 1.5% of alkaline protease based on the weight of the chicken, and enzymatically hydrolyze for 3 hours under magnetic stirring;

[0063] (2) Adjust the pH of the enzymatic hydrolysate to 8.0, the temperature of the water bath heating is 37 °C, add 1.8% of trypsin based on the weight of the chicken, and enzymatically hydrolyze for 2.5 hours under magnetic stirring;

[0064] (3) Adjust the pH of the enzymatic hydrolysate to 6.5, the temperature of the water bath heating is 42 °C, add 0.6% of neutral protease based on the weight of the chicken, and enzymatically hydrolyze for 1.5 hours under magnetic stirring;

[0065] (4) After the enzymatic hydrolysis is completed, filter the enzymatic hydrolysate with an ultrafiltration membrane with a molecular weight cut-off of 4000 Da to obtain a filtrate;

[0066] (5) Adjust the pH of the filtrate to 5.0, add 4 wt% of glucose to the filtrate, and then add probiotic liquid at an inoculation amount of 2 wt%. Ferment at 38 °C for 24 hours to obtain a fermentation broth; wherein, the probiotic is Lactobacillus acidophilus CICC 20250, and its liquid concentration is 5×10 8 CFU / mL;

[0067] (6) Freeze-dry the obtained fermentation broth at -80 °C for 48 hours to obtain chicken protein peptide powder.

[0068] Example 3

[0069] This embodiment provides a composition with anti-fatigue and uric acid-lowering effects, comprising the following components by weight: 30 parts of chicken protein peptide powder, 10 parts of marine fish oligopeptide powder, 3 parts of houttuynia powder, 5 parts of chicory powder, 5 parts of poria powder, 3 parts of sophora japonica powder, 2 parts of amomum villosum powder, 3 parts of prickly pear cactus juice powder, and 2 parts of tart cherry fruit powder.

[0070] Among them, the preparation method of the chicken protein peptide powder includes the following steps:

[0071] (1) Freeze the chicken and then crush it, add deionized water according to a ratio of 1:12, heat it in a water bath to 45 °C, adjust the pH to 9.0, add 1.0% of alkaline protease based on the weight of the chicken, and enzymatically hydrolyze for 3 hours under magnetic stirring;

[0072] (2) Adjust the pH of the enzymatic hydrolysate to 8.0, heat it in a water bath at 37 °C, add 1.6% of trypsin based on the weight of the chicken, and enzymatically hydrolyze for 2.5 hours under magnetic stirring;

[0073] (3) Adjust the pH of the enzymatic hydrolysate to 6.5, heat it in a water bath at 42 °C, add 1.5% of neutral protease based on the weight of the chicken, and enzymatically hydrolyze for 1.5 hours under magnetic stirring;

[0074] (4) After the enzymatic hydrolysis is completed, filter the enzymatic hydrolysate with an ultrafiltration membrane with a molecular weight cut-off of 3000 Da to obtain a filtrate;

[0075] (5) Adjust the pH of the filtrate to 5.0, add 4 wt% of galactooligosaccharide to the filtrate, and then add probiotic bacterial liquid at an inoculation amount of 2 wt%, ferment at 38 °C for 24 hours to obtain a fermentation broth; among them, the probiotic is Lactobacillus acidophilus CICC20250, and the concentration of its bacterial liquid is 5×10 8 CFU / mL;

[0076] (6) Freeze-dry the obtained fermentation broth at -80 °C for 48 hours to obtain chicken protein peptide powder.

[0077] Example 4

[0078] This embodiment provides a composition with anti-fatigue and uric acid-lowering effects, which is different from Example 1 in that the chicken protein peptide powder used is commercially available chicken concentrated powder.

[0079] Comparative Example 1

[0080] This comparative example provides a composition with anti-fatigue and uric acid-lowering effects, which is different from Example 1 in that the chicken protein peptide powder is not added, and correspondingly, 36 parts of marine fish oligopeptide powder are added more.

[0081] Comparative Example 2

[0082] This comparative example provides a composition with anti-fatigue and uric acid-lowering effects, which is different from Example 1 in that: houttuynia powder is not added, and correspondingly, 4 parts of chicory powder are added more.

[0083] Comparative Example 3

[0084] This comparative example provides a composition with anti-fatigue and uric acid-lowering effects, which is different from Example 1 in that: prickly pear cactus juice powder is not added, and correspondingly, 5 parts of amomum villosum powder are added more.

[0085] Test Example 1: Uric acid-lowering experiment

[0086] Experimental animals: Healthy adult male C57BL / 6 mice were selected, and their body weights were controlled at 18 - 22 g. The mice were raised in an environment with a temperature of (22 ± 2) °C and a humidity of (50 ± 10)%, maintaining a 12 h light - 12 h dark circadian rhythm, and having free access to food and water. The experiment started after 7 days of environmental adaptation.

[0087] Grouping: The total number of mice was 90, and they were finally divided into 9 groups: normal group, model group, experimental groups (Examples 1 - 4, Comparative Examples 1 - 3), with 10 mice in each group.

[0088] Modeling method: A hyperuricemia model in mice was constructed by intragastric administration of potassium oxonate (300 mg / kg) + hypoxanthine (500 mg / kg).

[0089] Normal group: Intragastric administration of 0.3 mL of distilled water for 4 consecutive weeks, without modeling treatment.

[0090] Model group: Intragastric administration of 0.1 mL of potassium oxonate solution + 0.1 mL of hypoxanthine solution. After 1 week, start intragastric administration of 0.1 mL of potassium oxonate solution + 0.1 mL of hypoxanthine solution + 0.1 mL of distilled water for 3 consecutive weeks.

[0091] Experimental groups: Intragastric administration of 0.1 mL of potassium oxonate solution + 0.1 mL of hypoxanthine solution. After 1 week, start intragastric administration of 0.1 mL of potassium oxonate solution + 0.1 mL of hypoxanthine solution + 0.1 mL of composition solution (dissolve the compositions of the examples and comparative examples at a dose of 1 mg / kg in 0.1 mL of distilled water) for 3 consecutive weeks.

[0092] 2 hours after intraperitoneal injection of potassium oxonate, blood was taken from the orbital cavity of the mice, left to stand at room temperature for 2 hours, centrifuged at 3500 r / min at low temperature for 10 minutes, the supernatant was taken and placed in a cryopreservation tube, and stored in a -80 °C ultra-low temperature refrigerator for standby. The uric acid content, urea nitrogen content, and xanthine oxidase activity in the serum were detected according to the operation steps of the uric acid kit, urea nitrogen kit, and xanthine oxidase kit instructions respectively.

[0093] Data statistics: Statistical processing was performed using SPSS 10.0 software, and the data were... It is indicated that the t - test is used for between - group comparison, and the analysis of variance is used for comparison of means among multiple groups. When P ≤ 0.05, it indicates significant differences.

[0094] Table 1 Results of uric acid level, urea nitrogen level and xanthine oxidase activity in the serum of hyperuricemia mice

[0095]

[0096]

[0097] Note: Compared with the normal group, # P < 0.05, ## P < 0.01; compared with the model group, * P < 0.05, ** P < 0.01.

[0098] Please refer to Table 1. After intragastric administration of the compositions of Examples 1 - 4, the contents of uric acid, urea nitrogen and xanthine oxidase in the serum of mice all showed significant decreases, indicating that the compositions provided in this example have good uric - acid - lowering effects. Among them, the composition of Example 4 uses commercially available chicken concentrated powder, and its uric - acid - lowering effect is worse than that of Example 1, proving that the chicken protein peptide powder prepared by the method recorded in the present invention has an obvious promoting effect on uric - acid lowering.

[0099] Compared with Example 1, after intragastric administration of the compositions of Comparative Examples 1 - 3, the contents of uric acid, urea nitrogen and xanthine oxidase in the serum of mice all showed significant decreases. This indicates that in the compositions of the present invention, components such as chicken protein peptide powder, marine fish oligopeptide powder, houttuynia cordata powder, chicory powder, poria cocos powder, sophora japonica powder, amomum villosum powder, prickly pear cactus fruit juice powder and tart cherry fruit powder can synergistically reduce uric acid, and the lack of any one of these components will lead to a significant reduction in the uric - acid - lowering effect.

[0100] Experimental Example 2: Anti - fatigue experiment

[0101] Experimental animals: Healthy adult male C57BL / 6 mice with body weight controlled at 18 - 22 g were selected. The fatigue model was constructed by the weight - bearing swimming experiment, and the swimming time of the mice until exhaustion was 300 s - 600 s as the fatigue model.

[0102] Grouping: The total number of mice was 100, and they were finally divided into 10 groups: normal control group (non - modeled mice), model group, positive control group, experimental groups (Examples 1 - 4, Comparative Examples 1 - 3), with 10 mice in each group.

[0103] The experimental groups were intragastrically administered with each composition (80 mg / kg body weight), the positive control group was intragastrically administered with ginsenoside (80 mg / kg body weight), and the model group and the normal control group were administered with normal saline. The administration was continued for 28 days. 30 minutes after the last administration, the mice were placed in a swimming tank for weight-bearing swimming (a lead block equivalent to 5% of the rat's body weight was attached to the rat's tail), and the swimming exhaustion time of the rats (i.e., the rats sank to the bottom and could not surface for more than 10 seconds) was recorded. At the same time, the blood of the mice was collected immediately after swimming to detect fatigue-related indicators such as blood lactic acid (BLA) and lactate dehydrogenase (LDH).

[0104] Data statistics: Statistical processing was performed using SPSS 10.0 software. The data were expressed as , and the t-test was used for comparison between groups. One-way ANOVA was used for comparison of means among multiple groups. When P≤0.05, it indicated a significant difference.

[0105] Table 2 Results of mouse fatigue test

[0106] Swimming time (min) LDH (U / L) LA (nmol / L) Normal control group 41.77±2.52 368.23±18.26 0.82±0.07 Model group <![CDATA[11.23±1.37 ## > <![CDATA[437.10±27.94 ## > <![CDATA[1.52±0.18 ## > Positive control group <![CDATA[43.03±3.78 ** > <![CDATA[374.14±34.63 * > <![CDATA[0.81±0.16 * > Example 1 <![CDATA[43.24±2.84 ** > <![CDATA[361.29±19.38 * > <![CDATA[0.73±0.18 * > Example 2 <![CDATA[39.89±3.20 ** > <![CDATA[372.30±31.03 * > <![CDATA[0.77±0.06 ** > Example 3 <![CDATA[40.21±2.00 ** > <![CDATA[366.03±23.63 * > <![CDATA[0.84±0.11 ** > Example 4 <![CDATA[32.09±1.78 ** > 391.20±15.34 <![CDATA[1.02±0.15 * > Comparative example 1 <![CDATA[29.93±1.97 ** > 389.12±22.87 1.20±0.11 Comparative example 2 <![CDATA[31.77±0.95 ** > 392.31±27.29 1.23±0.19 Comparative example 3 <![CDATA[32.37±3.28 ** > 396.19±29.04 1.19±0.08

[0107] Note: Compared with the normal control group, # P<0.05, ## P<0.01; compared with the model group, * P<0.05, ** P<0.01.

[0108] Please refer to Table 2. Compared with the model group, after intragastric administration of the compositions of Examples 1-4, the swimming time of the mice was significantly prolonged, and the contents of blood lactic acid (BLA) and lactate dehydrogenase (LDH) were significantly decreased, indicating that the compositions of the present invention can improve the anti-fatigue ability of mice. Among them, the composition of Example 4 uses commercially available chicken concentrated powder, and its anti-fatigue effect is worse than that of Example 1, proving that the chicken protein peptide powder prepared by the method described in the present invention has an obvious promoting effect on anti-fatigue.

[0109] Compared with Example 1, after intragastric administration of the compositions of Comparative Examples 1-3, the swimming time of the mice was significantly decreased, and the contents of blood lactic acid (BLA) and lactate dehydrogenase (LDH) were significantly increased. This indicates that in the compositions of the present invention, components such as chicken protein peptide powder, marine fish oligopeptide powder, houttuynia powder, chicory powder, poria powder, sophora flower powder, amomum powder, prickly pear cactus fruit juice powder, and tart cherry fruit powder can synergistically improve the anti-fatigue ability, and the lack of any one of these components will cause a significant decrease in the anti-fatigue effect.

[0110] The above-described embodiments are only preferred embodiments given to fully illustrate the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or transformations made by those skilled in the art on the basis of the present invention are all within the protection scope of the present invention. The protection scope of the present invention shall be subject to the claims.

Claims

1. A composition with the effects of anti-fatigue and reducing uric acid, characterized in that, It comprises the following components by weight parts: 0.1 - 50 parts of chicken protein peptide powder, 0.1 - 12 parts of marine fish oligopeptide powder, 1 - 11 parts of houttuynia cordata powder, 4 - 10 parts of chicory powder, 2 - 10 parts of poria cocos powder, 2 - 10 parts of sophora japonica powder, 2 - 10 parts of fructus amomi powder, 2 - 6 parts of prickly pear cactus juice powder, 1 - 5 parts of tart cherry fruit powder.

2. The composition with anti-fatigue and uric acid-lowering effects according to claim 1, characterized in that, The mass ratio of the chicken protein peptide powder to the marine fish oligopeptide powder is 2:1 - 5:

1.

3. A composition with anti-fatigue and uric acid-lowering effects according to claim 1, characterized in that, It comprises the following components by weight parts: 30 - 40 parts of chicken protein peptide powder, 10 - 15 parts of marine fish oligopeptide powder, 2 - 6 parts of houttuynia cordata powder, 5 - 8 parts of chicory powder, 4 - 8 parts of poria cocos powder, 2 - 6 parts of sophora japonica powder, 2 - 6 parts of fructus amomi powder, 3 - 6 parts of prickly pear cactus juice powder, 3 - 5 parts of tart cherry fruit powder.

4. A composition with anti - fatigue and uric acid - lowering effects according to claim 1, characterized in that, It comprises the following components by weight parts: 36 parts of chicken protein peptide powder, 12 parts of marine fish oligopeptide powder, 4 parts of houttuynia cordata powder, 6 parts of chicory powder, 6 parts of poria cocos powder, 4 parts of sophora japonica powder, 4 parts of fructus amomi powder, 5 parts of prickly pear cactus juice powder, 4 parts of tart cherry fruit powder.

5. A composition with anti-fatigue and uric acid-lowering effects according to claim 1, characterized in that, The preparation method of the chicken protein peptide powder is as follows: Freeze the chicken and then pulverize it, mix it with water and heat, then add protease for segmented enzymatic hydrolysis; after the enzymatic hydrolysis ends, filter the enzymatic hydrolysate with an ultrafiltration membrane; then, adjust the pH to acidic, add saccharide substances to the filtrate, and add probiotic bacterial liquid for fermentation; the obtained fermentation broth is freeze-dried to obtain chicken protein peptide powder.

6. The composition with anti-fatigue and uric acid-lowering effects according to claim 5, characterized in that The segmented enzymatic hydrolysis includes the first-stage enzymatic hydrolysis, the second-stage enzymatic hydrolysis and the third-stage enzymatic hydrolysis; For the first-stage enzymatic hydrolysis, the protease used is alkaline protease, the pH value is adjusted to 8 - 10, the temperature is controlled at 40°C - 50°C, and the enzymatic hydrolysis is carried out for 2 - 3 hours; for the second-stage enzymatic hydrolysis, the protease used is trypsin, the pH value is adjusted to 7.5 - 8.5, the temperature is controlled at 37°C, and the enzymatic hydrolysis is carried out for 2 - 3 hours; for the third-stage enzymatic hydrolysis, the protease used is neutral protease, the pH value is adjusted to 6 - 7, the temperature is controlled at 40°C - 45°C, and the enzymatic hydrolysis is carried out for 1 - 2 hours.

7. A composition having the effects of anti-fatigue and reducing uric acid according to claim 6, characterized in that During the first-stage enzymatic hydrolysis, the addition amount of alkaline protease is 0.8 - 1.5% of the weight of the chicken; During the second-stage enzymatic hydrolysis, the addition amount of trypsin is 1 - 2% of the weight of the chicken; During the third-stage enzymatic hydrolysis, the addition amount of neutral protease is 0.2 - 1% of the weight of the chicken.

8. A composition with anti-fatigue and uric acid-lowering effects according to claim 6, characterized in that The preparation method of the chicken protein peptide powder also satisfies any one of the following: (1) The mass ratio of the chicken to the water is 1:8 - 12; (2) The cut-off molecular weight of the ultrafiltration membrane is 3000 - 5000 Da; (3) The pH is adjusted to 4.5 - 6.5; (4) The saccharide substances include at least one of glucose, fructooligosaccharide, galactooligosaccharide, and the addition amount is 2 - 5% of the weight of the filtrate; (5) The probiotic is Lactobacillus acidophilus CICC 20250, and its concentration is (1-6)×10 8 CFU / mL, and the inoculation amount is 1-3%; (6) The fermentation temperature is 35 - 40°C, and the fermentation time is 12 - 24 hours; (7) The temperature of the freeze-drying is -60 - 80°C, and the freeze-drying time is 24 - 48 h.

9. A health food, characterized in that, The health food contains the composition with the effects of anti-fatigue and uric acid reduction described in any one of claims 1 - 8.

10. The health food according to claim 9, wherein, It also includes excipients, and the excipients include at least one of fillers, acidulants, diluents and thickeners.

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