Anaerobic ammonium oxidation biofilm culture system and culture method

Abstract

The present invention provides an anaerobic ammonia oxidation biofilm culture system and culture method, which belongs to the technical field of sewage treatment. The culture system includes a reactor, a storage tank, a drive module, a reciprocating base and a plurality of carrier assemblies; the interior of the reactor includes a nitrogen protection zone located in the upper layer and a culture zone and an operation zone located in the lower layer; the storage tank is fixed to the outer peripheral wall of the reactor, and the side wall of the operation zone is provided with a connecting opening connected to the storage tank; a plurality of carrier assemblies are installed in the culture zone, the reciprocating base is installed in the storage tank, the drive module is fixed to the nitrogen protection zone, the reciprocating base is used to drive the carrier assembly to move between the storage tank and the operation zone, and the drive module is used to clamp the carrier assembly to move between the culture zone and the reciprocating base located in the operation zone. The present application utilizes the culture system to carry out targeted pre-culture of anaerobic ammonia oxidation biofilms for different pollution sources, saving the culture time of the anaerobic ammonia oxidation biofilms and assisting the sewage treatment system to be put into production quickly.

Description

Technical Field

[0001] The present invention belongs to the technical field of sewage treatment, and in particular relates to an anaerobic ammonia oxidation biofilm culture system and a culture method. Background Art

[0002] In the field of anaerobic ammonium oxidation (Anammox) technology and engineering applications, anaerobic ammonium oxidizing bacteria (AnAOB) suffer from slow growth and difficulty in enrichment. Furthermore, to improve wastewater treatment effectiveness, it is generally necessary to cultivate Anammox biofilms tailored to the pollution source. Therefore, when constructing a wastewater treatment system, a complete wastewater treatment system is typically designed first, targeting the pollution source. Then, the corresponding tanks and pipelines are constructed based on the designed wastewater treatment system. However, the Anammox biofilms required for the wastewater treatment system are introduced only after the tanks and pipelines are constructed. During the Anammox biofilm cultivation period, the wastewater cannot be effectively treated, which prolongs the time it takes to commission the wastewater treatment system.

[0003] In addition, the maintenance cost of the Anammox reactor in the sewage treatment system will increase year by year due to scaling, bacterial imbalance, and increased monitoring needs. Summary of the Invention

[0004] The purpose of this application is to provide an anaerobic ammonia oxidation biofilm culture system and culture method to solve the above-mentioned technical problems existing in the prior art.

[0005] This application is implemented as follows:

[0006] In the first aspect, an embodiment of the present application provides an anaerobic ammonia oxidation biofilm culture system, comprising a reactor, a storage tank, a drive module, a reciprocating base and a plurality of carrier assemblies; the interior of the reactor comprises a nitrogen protection zone located on the upper layer and a culture zone and an operation zone located on the lower layer, an isolation plate is provided between the culture zone and the operation zone, the nitrogen protection zone has an air inlet and an air outlet, and the culture zone has a liquid inlet and a liquid outlet; the storage tank is fixed to the outer peripheral wall of the reactor, the top of the storage tank is open, and the side wall of the operation zone is provided with a connecting opening connected to the storage tank; a plurality of carrier assemblies are installed in the culture zone, the reciprocating base is installed in the storage tank, the drive module is fixed to the nitrogen protection zone, the reciprocating base is used to move uncultured carrier assemblies from the storage tank to the operation zone, and to move cultured carrier assemblies from the operation zone to the storage tank, and the drive module is used to clamp the carrier assemblies and move them between the culture zone and the reciprocating base located in the operation zone.

[0007] In the second aspect, an embodiment of the present application provides an anaerobic ammonia oxidation biofilm culture method, using the anaerobic ammonia oxidation biofilm culture system provided by the embodiment of the first aspect, and the specific steps include: placing an uncultured carrier assembly on a reciprocating base in a storage tank, and driving the reciprocating base to an operating area; starting a drive module, clamping the carrier assembly in the operating area, moving the carrier assembly and placing the carrier assembly in the culture area; introducing target sewage into the culture area, introducing nitrogen into the nitrogen protection area, and introducing sewage into the storage tank; starting a drive module, clamping the cultured carrier assembly in the culture area, moving the carrier assembly and placing the carrier assembly on the reciprocating base in the operating area, driving the reciprocating base to move to the storage tank, and taking out the cultured carrier assembly.

[0008] The technical solution adopted by the present invention can achieve the following beneficial effects:

[0009] In this application, a culture system is used to pre-cultivate anaerobic ammonium oxidation biofilms for different pollution sources. Compared with the existing technology, this saves the culture time of the anaerobic ammonium oxidation biofilm, thereby assisting the rapid commissioning of the sewage treatment system. It can also directly replace the later anaerobic ammonium oxidation reactors in the sewage treatment system, thereby reducing the overall maintenance cost of the sewage treatment system.

[0010] In the culture system, the interior of the reactor is divided into a nitrogen protection area, a culture area and an operation area. The operation area is connected to the storage tank. A carrier assembly is placed in the culture area for the cultivation of anaerobic ammonia oxidation biofilms. With the help of a drive module and a reciprocating base, the cultured carrier assembly is moved to the storage tank and taken out, and then transferred to the sewage treatment system for sewage treatment. The uncultured carrier assembly is moved from the storage tank to the culture area for cultivation, thereby realizing uninterrupted cultivation of anaerobic ammonia oxidation biofilms.

[0011] By introducing sewage into the storage pool, the operating area can be sealed. During the process of transferring the carrier assembly, the oxygen content in the culture area can be kept stable, avoiding the impact of the external environment on the culture area. At the same time, the storage pool with sewage can ensure the stability of the environment in which the cultured carrier assembly is located, and can provide a free and safe operating space for the transfer of the carrier assembly, and can also ensure that the carrier assembly can be protected and removed in an appropriate environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments of the present invention or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0013] Figure 1 This is a schematic diagram of the overall structure of the culture system provided in some embodiments of the present application. Figure 1 ;

[0014] Figure 2 This is a schematic diagram of the overall structure of the culture system provided in some embodiments of the present application. Figure 2 ;

[0015] Figure 3 This is a cross-sectional view of the culture system provided in some embodiments of the present application. Figure 1 ;

[0016] Figure 4 This is a cross-sectional view of the culture system provided in some embodiments of the present application. Figure 2 ;

[0017] Figure 5 is a cross-sectional view of a reactor and a storage tank provided in some embodiments of the present application;

[0018] Figure 6 is a schematic diagram of the internal structure of the reactor and storage tank provided in some embodiments of the present application;

[0019] Figure 7 is a schematic diagram of the overall structure of the carrier assembly provided in some embodiments of the present application;

[0020] Figure 8 is a cross-sectional view of a carrier assembly provided in some embodiments of the present application;

[0021] Figure 9 This application is about Figure 8 Detailed view of point A;

[0022] Figure 10 is a cross-sectional view of a carrier assembly and a packaging tube provided in some embodiments of the present application;

[0023] Figure 11 This application is about Figure 10 Detail of point B;

[0024] Figure 12 This is a schematic diagram of the structure of the drive module provided in some embodiments of the present application.

[0025] In the figure: 100-reactor, 110-nitrogen protection zone, 111-air inlet, 112-air outlet, 120-culture area, 121-liquid inlet, 122-liquid outlet, 130-operation area, 140-isolation plate, 150-connecting opening, 200-storage tank, 300-drive module, 310-track frame, 320-clamp, 400-reciprocating base, 410-first type base, 420-second type base, 500- Carrier assembly, 510-load rod, 511-air hole, 520-cover plate, 530-carrier, 540-air bag, 550-movable structure, 551-elastic part, 552-sliding block, 600-packaging tube, 610-air release groove, 620-limiting plate, 700-mounting base, 710-inflating groove, 720-sealing film, 810-nitrogen delivery pipe, 820-sewage delivery pipe, 900-fixing bracket, 910-mounting hole. DETAILED DESCRIPTION

[0026] To make the objectives, technical solutions, and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other implementations obtained by those of ordinary skill in the art without inventive effort are within the scope of protection of the present invention.

[0027] An embodiment of the present application provides an anaerobic ammonium oxidation biofilm culture system, including a reactor 100 , a storage tank 200 , a drive module 300 , a reciprocating base 400 , and a plurality of carrier assemblies 500 .

[0028] The reactor 100 serves as the main container for anaerobic ammonium oxidation biofilm cultivation. Its interior is hollow and includes a nitrogen protection zone 110 located on the upper layer and a culture zone 120 and an operation zone 130 located on the lower layer. The reactor 100 is placed on a horizontal plane when in use. An isolation plate 140 is provided between the culture zone 120 and the operation zone 130, which separates the culture zone 120 and the operation zone 130. The nitrogen protection zone 110 has an air inlet 111 and an air outlet 112. Nitrogen enters the nitrogen protection zone 110 through the air inlet 111 and is discharged through the air outlet 112, providing a cultivation environment for the anaerobic ammonium oxidation biofilm. The culture zone 120 has a liquid inlet 121 and a liquid outlet 122. The target sewage is discharged into the culture zone 120 from the liquid inlet 121 and is discharged from the liquid outlet 122. The specific positions of the air inlet 111, the air outlet 112, the liquid inlet 121 and the liquid outlet 122 can be referred to Figure 1 and Figure 2 shown.

[0029] The storage tank 200 is fixed to the outer wall of the reactor 100 and has an open top. An operator can operate the interior of the storage tank 200 through the top opening of the storage tank 200. The sidewall of the operation area 130 is provided with a communication opening 150 that communicates with the storage tank 200, and the interior of the storage tank 200 is connected to the operation area 130. The sidewall of the operation area 130 refers to the sidewall of the reactor 100 corresponding to the operation area 130.

[0030] Multiple carrier assemblies 500 are installed in the culture area 120, where they are cultured. The reciprocating base 400 is installed in the storage tank 200, and the driving module 300 is fixed to the nitrogen protection zone 110. The driving module 300 is actually fixed to the inner wall surface of the reactor 100 corresponding to the nitrogen protection zone 110.

[0031] The reciprocating base 400 moves back and forth between the storage pool 200 and the operating area 130. The reciprocating base 400 is used to move the uncultured carrier assembly 500 from the storage pool 200 to the operating area 130, and to move the cultured carrier assembly 500 from the operating area 130 to the storage pool 200. The driving module 300 is used to clamp the carrier assembly 500 and move it between the culture area 120 and the reciprocating base 400 located in the operating area 130.

[0032] An isolation plate 140 is provided between the operation area 130 and the culture area 120. The reciprocating base 400 cannot move the carrier assembly 500 horizontally from the operation area 130 to the culture area 120. Therefore, an additional driving module 300 is required. The driving module 300 is used to clamp the carrier assembly 500. The nitrogen protection area 110 provides a movable space for the driving module 300, so that the driving module 300 can clamp the carrier assembly 500 and change its position between the operation area 130 and the culture area 120 through the nitrogen protection area 110.

[0033] After the anaerobic ammonia oxidation biofilm is cultured on the surface of the carrier assembly 500, the carrier assembly 500 needs to be removed and transferred to a sewage treatment system that needs to be treated. The specific removal steps are: first, use the driving module 300 to clamp the cultured carrier assembly 500, drive the carrier assembly 500 to rise to the nitrogen protection zone 110, then move the carrier assembly 500 above the operating area 130, lower the carrier assembly 500 into the operating area 130, place the carrier assembly 500 on the reciprocating base 400 in the operating area 130, move the reciprocating base 400, drive the carrier assembly 500 to move into the storage tank 200, and the operator takes out the cultured carrier assembly 500 through the top opening of the storage tank 200 and transfers it to the sewage treatment system.

[0034] After the cultured carrier assembly 500 is taken out, it is necessary to place an uncultured carrier assembly 500 in an empty space to make full use of the culture system and realize uninterrupted culture of the anaerobic ammonia oxidation biofilm. The specific steps are as follows: the operator places the uncultured carrier assembly 500 on the reciprocating base 400 at the bottom of the storage tank 200 through the top opening of the storage tank 200, and the reciprocating base 400 moves toward the operating area 130 to move the carrier assembly 500 to the operating area 130. The driving module 300 clamps the carrier assembly 500, drives the carrier assembly 500 to rise to the nitrogen protection area 110, and then moves the carrier assembly 500 to above the culture area 120, and lowers the carrier assembly 500 into the culture area 120.

[0035] In the embodiment of the present application, the interior of the reactor 100 is divided into a nitrogen protection area 110, a culture area 120 and an operation area 130. The operation area 130 is connected to the storage tank 200. The reciprocating base 400 and the driving module 300 cooperate to complete the replacement of the carrier component 500 in the culture area 120, thereby achieving uninterrupted cultivation of the anaerobic ammonia oxidation biofilm and providing a basis for the replacement of the carrier component 500 in the later sewage treatment system.

[0036] The cultivation system provided in the embodiment of the present application can pre-cultivate anaerobic ammonium oxidation biofilms targeted at different pollution sources, and cultivate anaerobic ammonium oxidation biofilms during the construction phase of the sewage treatment system. After the construction of the sewage treatment system is completed, the anaerobic ammonium oxidation biofilms cultivated in the cultivation system can be used to treat the sewage. Compared with the existing technology, the cultivation time of the anaerobic ammonium oxidation biofilm is saved, thereby shortening the commissioning time of the sewage treatment system and assisting the sewage treatment system to be put into production quickly.

[0037] After taking out the cultured carrier assembly 500, a new carrier assembly 500 can be put into the culture system. When the anaerobic ammonium oxidation reactor in the sewage treatment system needs to be maintained in the future, the carrier assembly 500 cultured in the culture system can be directly used to replace the anaerobic ammonium oxidation reactor, thereby saving maintenance time and reducing the overall maintenance cost of the sewage treatment system.

[0038] Furthermore, in the culture system provided in the embodiment of the present application, the operation area 130 is connected to the storage tank 200. By introducing corresponding sewage into the storage tank 200, when the cultured carrier assembly 500 is taken out from the culture area 120, the carrier assembly 500 is prevented from being contaminated by the external environment. At the same time, the sewage in the storage tank 200 simultaneously fills the operation area 130, and the communication opening 150 between the operation area 130 and the storage tank 200 can be sealed to prevent the influence of the external environment on the culture area 120, and the oxygen content in the culture area 120 can be kept stable. At the same time, the nitrogen protection zone 110 is also isolated from the external environment. In the process of transferring the cultured carrier assembly 500, a free and safe operating space is provided for the transfer of the carrier assembly 500. The cultured carrier assembly 500 is taken out of the storage tank 200, and the storage tank 200 is filled with sewage. The sewage is adapted to the anaerobic ammonia oxidation biofilm on the surface of the carrier assembly 500, ensuring that the carrier assembly 500 can be protected and taken out in an adapted environment.

[0039] During the anaerobic ammonium oxidation biofilm cultivation process, nitrogen needs to be introduced into the nitrogen protection zone 110. To save costs, nitrogen can be introduced intermittently. During the period when nitrogen is not introduced, the membrane structure is used to cover the sewage surface of the cultivation zone 120.

[0040] In some preferred embodiments, in the depth direction of the reactor 100, the top of the isolation plate 140 is higher than the upper edge of the communication opening 150. Figure 3 and Figure 5 After sewage is introduced into the storage tank 200 and the operation area 130, and the sewage is sealed through the opening 150, the sewage in the operation area 130 can be lower than the top of the isolation plate 140. The sewage in the operation area 130 and the sewage in the culture area 120 are isolated from each other, further ensuring the stability of the oxygen content of the sewage in the culture area 120.

[0041] In some preferred embodiments, the culture system further includes a sewage delivery pipe 820, the inlet of which is connected to the liquid outlet 122 of the culture area 120, and the outlet of which is connected to the storage tank 200. The sewage entering the storage tank 200 originates from the culture area 120, so that the sewage in the operation area 130 and the culture area 120 are as similar as possible, thereby ensuring the stability of the environment in which the cultured carrier assembly 500 is located and further improving the stability of the anaerobic ammonium oxidation biofilm formed on the surface of the carrier assembly 500.

[0042] In addition, the wastewater from culturing the anaerobic ammonia-oxidizing biofilm in the culture system is discharged into the storage tank 200. On the one hand, the utilization rate of the wastewater can be improved. On the other hand, there is no need to set up additional wastewater inlet equipment for the storage tank 200. The wastewater delivery pipe 820 is directly used to connect the storage tank 200 with the liquid outlet 122 of the culture area 120. The wastewater in the storage tank 200 can be replaced while the wastewater in the culture area 120 is discharged. Both the culture area 120 and the storage tank 200 discharge wastewater through the outlet on the storage tank 200, which is conducive to simplifying the overall structure of the culture system and simplifying the operating steps of the culture system.

[0043] In some preferred embodiments, there are at least two reciprocating bases 400, which can be referred to Figure 6 As shown, at least one reciprocating base 400 is used to move the uncultured carrier component 500 from the storage tank 200 to the operating area 130, and at least one reciprocating base 400 is installed with a packaging cylinder 600, which is used to move the cultured carrier component 500 from the operating area 130 to the storage tank 200, and the packaging cylinder 600 is used to accommodate the cultured carrier component 500.

[0044] In some embodiments, the specific structures of the reciprocating base 400 installed for the cultured carrier assembly 500 and the uncultured carrier assembly 500 are different. Figure 6 As shown, the reciprocating base 400 is divided into a first type base 410 and a second type base 420. For the uncultured carrier assembly 500, it is directly mounted on the first type base 410, referring to Figure 3 and Figure 4 As shown, it is driven by the first type base 410 and moves from the storage tank 200 to the operation area 130. For the carrier assembly 500 that has been cultured, the second type base 420 can be used, referring to Figure 6 As shown, the second type base 420 is used to cooperate with the packaging tube 600. The packaging tube 600 can be placed on the second type base 420. When the carrier assembly 500 is transferred from the culture area 120 to the operation area 130, the cultured carrier assembly 500 can be placed in the packaging tube 600 to complete the storage of the carrier assembly 500. The packaging tube 600 is located between the storage tank 200 and the operation area 130. The wastewater in the storage tank 200 and the operation area 130 can be passed into the packaging tube 600, maintaining the stability of the external environment corresponding to the cultured carrier assembly 500.

[0045] The packaging tube 600 is a cylindrical structure, with its bottom fixed to the second-type base 420 and its top open to allow the carrier assembly 500 to enter. The packaging tube 600 needs to pass through the communication opening 150 to move back and forth between the operating area 130 and the storage tank 200. The water level in the storage tank 200 is higher than the communication opening 150, so the sewage will fill the packaging tube 600.

[0046] In some preferred embodiments, the packaging tube 600 is installed on the second type base 420. Before it is moved to the operating area 130, the packaging tube 600 is located below the water inlet of the storage tank 200, further improving the similarity between the sewage in the packaging tube 600 and the sewage in the culture area 120.

[0047] In some embodiments, the reciprocating base 400 is connected to a drive mechanism that drives the reciprocating base 400 to move. The packaging tube 600 is directly mounted on the upper surface of the second-type base 420. The second-type base 420 can also surround the packaging tube 600 and be fixed to the peripheral wall of the packaging tube 600 to form a stable connection with the packaging tube 600.

[0048] The carrier assembly 500 includes a load beam 510, a cover plate 520, and a plurality of carriers 530. Figure 7 、 Figure 8 and Figure 11 As shown, the cover plate 520 and the plurality of carriers 530 are fixed to the load rod 510, and the cover plate 520 is located at the upper end of the load rod 510. The cover plate 520 can be snapped onto the opening of the packaging tube 600 to seal the packaging tube 600. The cooperation between the cover plate 520 and the packaging tube 600 can be referred to Figure 10 shown.

[0049] The anaerobic ammonium oxidation biofilm is mainly formed on the surface of the carrier 530. The carrier 530 can be selected from one or more of polyurethane foam, bio-belt, activated carbon, biochemical cotton, volcanic stone, natural zeolite, non-woven fabric, etc.

[0050] Preferably, the plurality of carriers 530 are evenly distributed along the axis of the loading rod 510, with gaps between adjacent carriers 530 to ensure sufficient contact between the carriers 530 and the sewage. The loading rod 510 is vertically installed in the culture area 120. The sewage in the culture area 120 flows horizontally, and the vertical loading rod 510 is conducive to intercepting the horizontally flowing sludge.

[0051] The carrier assembly 500 further includes an airbag 540 and a movable structure 550. The airbag 540 is mounted on the surface of the cover plate 520 close to the carrier 530. Figure 7 、 Figure 8 as well as Figure 10 As shown, the airbag 540 is made of elastic material, and the elastic airbag 540 can improve the sealing effect between the cover plate 520 and the packaging tube 600.

[0052] The movable structure 550 is movably mounted on the load rod 510, and multiple carriers 530 are located between the movable structure 550 and the airbag 540, with the airbag 540 located at the top and the movable structure 550 located at the bottom. The load rod 510 is hollow and connected to the airbag 540. The side wall of the load rod 510 is provided with an air hole 511 connected to the interior thereof, and the movable structure 550 blocks the air hole 511. The movable structure 550 blocks the air hole 511, blocking the gas exchange between the inside and outside of the load rod 510, and can seal the inside of the load rod 510. When the movable structure 550 does not block the air hole 511, the air hole 511 is connected to the outside, and the inside of the load rod 510 and the airbag 540 can be inflated or deflated through the air hole 511.

[0053] The culture system further includes a plurality of mounting bases 700, which are fixed to the bottom of the culture area 120. Figure 5 and Figure 6 As shown, the mounting base 700 is used to mount the carrier assembly 500. The mounting base 700 is provided with an air filling groove 710, referring to Figure 8 and Figure 9 As shown, a sealing film 720 is provided at the opening of the inflation groove 710 , and the inflation groove 710 is connected to a nitrogen delivery pipe 810 .

[0054] The carrier assembly 500 can be mounted to the mounting base 700 under the drive of the drive module 300, so that the load rod 510 passes through the sealing film 720 and the end of the load rod 510 enters the inflation groove 710. The movable structure 550 cannot pass through the sealing film 720 and enter the inflation groove 710, and is blocked by the sealing film 720 outside the inflation groove 710. The air hole 511 is located in the inflation groove 710 to connect the air bag 540 and the inflation groove 710.

[0055] During the installation of the carrier assembly 500 onto the mounting base 700, the load rod 510 can pass through the sealing film 720, but the movable structure 550 cannot. When the movable structure 550 is blocked by the sealing film 720, the movable structure 550 cannot continue to move, while the load rod 510 can continue to move. The movable structure 550 and the load rod 510 move relative to each other, and the load rod 510 continues to move toward the inflation groove 710. The air holes 511 on the load rod 510 gradually move away from the movable structure 550, and the movable structure 550 gradually cannot continue to block the air holes 511 until the air holes 511 enter the inflation groove 710, connecting the airbag 540 and the inflation groove 710. Nitrogen is then introduced into the inflation groove 710 through the nitrogen delivery pipe 810. The nitrogen enters the interior of the load rod 510 through the air holes 511 and then enters the airbag 540, inflating the airbag 540.

[0056] After inflation, the airbag 540 expands as a whole, generating a certain amount of buoyancy in the sewage. This facilitates the separation of the carrier assembly 500 from the mounting base 700 when the drive module 300 grips the carrier assembly 500. This also ensures that the cover plate 520 remains exposed above the sewage surface, facilitating its interaction with the drive module 300. Furthermore, during the separation process between the carrier assembly 500 and the mounting base 700, the movable structure 550 moves away from the sealing membrane 720 and can be reset to re-cover the air hole 511, sealing the airbag 540 and the interior of the load rod 510.

[0057] There are multiple mounting bases 700 , and each of the multiple mounting bases 700 is connected to a nitrogen delivery pipe 810 . The multiple nitrogen delivery pipes 810 are all connected to the same gas source and are supplied with gas by the same gas source.

[0058] refer to Figure 9 and Figure 11 As shown, the movable structure 550 includes an elastic member 551 and a sliding block 552 that are sleeved on the outside of the load rod 510. One end of the elastic member 551 is fixed to the load rod 510, and the other end is fixed to the sliding block 552. The sliding block 552 is located on the side of the elastic member 551 away from the cover plate 520, and the sliding block 552 blocks the air hole 511. When the carrier assembly 500 is installed on the mounting base 700, the sliding block 552 can slide relative to the load rod 510 under the obstruction of the sealing film 720 to compress the elastic member 551, thereby removing the obstruction of the air hole 511. When the carrier assembly 500 is removed from the mounting base 700, the load rod 510 gradually moves away from the sealing film 720, and the compressed elastic member 551 drives the sliding block 552 to return to its original position, thereby blocking the air hole 511.

[0059] refer to Figure 11 As shown, the packaging tube 600 is provided with a degassing groove 610 at its bottom, and a limiting plate 620 is provided at the opening of the degassing groove 610. The limiting plate 620 is provided with a through hole connecting the interior of the degassing groove 610 with the interior of the packaging tube 600. The carrier assembly 500 can be installed into the packaging tube 600 under the drive of the driving module 300, so that the load rod 510 passes through the limiting plate 620, and the movable structure 550 is blocked outside the degassing groove 610 by the limiting plate 620. The air hole 511 is located in the degassing groove 610 to connect the degassing groove 610 and the air bag 540.

[0060] The cooperation between the carrier assembly 500 and the limiting plate 620 is equivalent to the cooperation between the carrier assembly 500 and the sealing film 720. The load rod 510 is inserted into the degassing groove 610, and the air hole 511 connects the degassing groove 610 and the air bag 540. The nitrogen in the air bag 540 can enter the degassing groove 610 and enter the interior of the packaging tube 600 through the through hole on the limiting plate 620, thereby providing nitrogen protection for the carrier assembly 500 in the packaging tube 600.

[0061] The driving module 300 provided in the embodiment of the present application is used to clamp the carrier assembly 500 and drive the carrier assembly 500 to move. In some embodiments, the driving module 300 includes a track frame 310 and a clamp 320. Figure 12 As shown, the track frame 310 is fixed to the top wall of the nitrogen protection zone 110, and the clamp 320 is movably connected to the track frame 310. The clamp 320 can move along the depth direction of the reactor 100 to clamp or place the carrier assembly 500. The depth direction of the reactor 100 is the direction of gravity. The clamp 320 moves up and down along the direction of gravity to remove the carrier assembly 500 from the culture area 120 or the operation area 130 or to place the carrier assembly 500 in the culture area 120 or the operation area 130.

[0062] In some embodiments of this application, reference may be made to Figure 5 As shown, the culture area 120 is mounted with a fixing frame 900, which is provided with multiple mounting holes 910. The carrier assembly 500 is inserted into the mounting holes 910, thereby restricting the installation position of the carrier assembly 500. When the culture area 120 is provided with a mounting base 700, the fixing frame 900 cooperates with the mounting base 700 to improve the positional stability of the carrier assembly 500 in the culture area 120. The hollow design of the fixing frame 900 does not affect the contact between the nitrogen in the nitrogen protection area 110 and the wastewater in the culture area 120.

[0063] The present application provides an anaerobic ammonium oxidation biofilm culture method, using the anaerobic ammonium oxidation biofilm culture system provided in any of the above embodiments, and the specific culture steps include:

[0064] First, the uncultured carrier assembly 500 is placed on the reciprocating base 400 in the storage tank 200 , and the reciprocating base 400 is driven into the operating area 130 .

[0065] Then, the driving module 300 is started, and the carrier assembly 500 in the operation area 130 is clamped by the driving module 300 , and the carrier assembly 500 is moved and placed in the culture area 120 .

[0066] Before introducing sewage, it is necessary to install uncultured carrier assemblies 500 into the culture area 120. The above steps can be repeated until enough carrier assemblies 500 are installed in the culture area 120.

[0067] After the carrier assembly 500 is installed, the target sewage can be introduced into the culture area 120, nitrogen can be introduced into the nitrogen protection area 110, and sewage can be introduced into the storage tank 200 to culture the anaerobic ammonium oxidation biofilm.

[0068] In some specific embodiments, the mixed activated sludge of ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria can be used as the inoculation source (the inoculation sludge concentration is 4000-5000 mg / L), the actual pretreated food waste fermentation liquid is used as the influent, and the influent is subjected to the conditions of oxygen limitation (DO≤0.5 mg / L), temperature ≥27°C, pH=7.5-8.5, and influent ammonia nitrogen load of 0.032 kg / (m 3 ·d) The total nitrogen load of the influent is 0.04 kg / (m 3 ·d) and continue to operate until the carrier 530 has completed the biofilm formation and the treatment effect is stable. After that, the total nitrogen load is gradually increased to 0.08kg / (m 3 ·d)、0.1kg / (m 3 ·d)、0.12kg / (m 3 ·d)、0.15kg / (m 3 ·d)、0.18kg / (m 3 ·d)、0.21kg / (m 3 ·d)、0.25kg / (m 3 ·d)、0.3kg / (m 3 ·d)、0.35kg / (m 3 ·d)、0.4kg / (m 3 d) Cultivating under each operating condition for 2-4 weeks until the anaerobic ammonium oxidation biofilm in the reactor 100 is fully adapted and highly active, that is, the total nitrogen removal rate is stably higher than 85%, and the cultivation is completed.

[0069] After the anaerobic ammonia oxidation biofilm culture is completed, the driving module 300 is started, the carrier assembly 500 that has been cultured in the culture area 120 is clamped, the carrier assembly 500 is moved and placed on the reciprocating base 400 in the operation area 130, and the reciprocating base 400 is driven to move to the storage tank 200, and the carrier assembly 500 that has been cultured is taken out.

[0070] The cultured carrier assembly 500 is moved to a sewage treatment system to treat the sewage. The sewage treatment system does not need to culture the anaerobic ammonia oxidation biofilm, which saves culture time and improves the overall efficiency of sewage treatment.

[0071] After the cultured carrier component 500 is taken out from the culture area 120, the uncultured carrier component 500 can be moved back to the culture area 120 for the next round of anaerobic ammonia oxidation biofilm culture, providing a basis for the replacement of the carrier component 500 in the subsequent sewage treatment system.

[0072] It should be noted that, in this document, the terms "comprises," "includes," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or apparatus comprising a series of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or apparatus comprising the element.

[0073] The above description is only a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with this technical field can easily think of changes or replacements within the technical scope disclosed by the present invention, which should be covered by the scope of protection of the present invention.

Claims

1. An anaerobic ammonium oxidation biofilm culture system, characterized in that: It includes a reactor, a storage tank, a driving module, a reciprocating base and a plurality of carrier components; The reactor comprises a nitrogen protection zone located on the upper layer and a culture zone and an operation zone located on the lower layer, an isolation plate is provided between the culture zone and the operation zone, the nitrogen protection zone has an air inlet and an air outlet, and the culture zone has a liquid inlet and a liquid outlet; The storage tank is fixed to the outer peripheral wall of the reactor, the top of the storage tank is open, and the side wall of the operation area is provided with a communication opening communicating with the storage tank; Multiple carrier assemblies are installed in the culture area, the reciprocating base is installed in the storage pool, and the driving module is fixed to the nitrogen protection zone. The reciprocating base is used to move the uncultured carrier assemblies from the storage pool to the operating area, and move the cultured carrier assemblies from the operating area to the storage pool. The driving module is used to clamp the carrier assemblies and move them between the culture area and the reciprocating base located in the operating area.

2. The anaerobic ammonium oxidation biofilm culture system according to claim 1, characterized in that: In the depth direction of the reactor, the top end of the isolation plate is higher than the upper edge of the communication opening.

3. The anaerobic ammonium oxidation biofilm culture system according to claim 1, characterized in that: There are at least two reciprocating bases, at least one of which is used to move the uncultured carrier component from the storage tank to the operating area, and at least one of which is equipped with a packaging cylinder for moving the cultured carrier component from the operating area to the storage tank, and the packaging cylinder is used to accommodate the cultured carrier component.

4. The anaerobic ammonium oxidation biofilm culture system according to claim 3, characterized in that: The carrier assembly includes a load rod, a cover plate, and a plurality of carriers. The cover plate and the plurality of carriers are fixed to the load rod, and the cover plate is located at the upper end of the load rod. The cover plate can be snapped onto the opening of the packaging tube to seal the packaging tube.

5. The anaerobic ammonium oxidation biofilm culture system according to claim 4, characterized in that: The carrier assembly further includes an airbag and a movable structure, wherein the airbag is mounted on a surface of the cover plate close to the carrier, and the movable structure is movably mounted on the load rod. The plurality of carriers are located between the movable structure and the airbag, and the load rod is hollow and communicates with the airbag. The sidewall of the load rod is provided with an air hole communicating with the interior thereof, and the movable structure blocks the air hole. The culture system further comprises a plurality of mounting bases, each of which is fixed to the bottom of the culture area and is used to mount the carrier assembly. An aeration groove is provided inside the mounting base, a sealing film is provided at the opening of the aeration groove, and the aeration groove is connected to a nitrogen delivery pipe. The carrier assembly can be installed to the mounting base under the drive of the driving module, so that the load rod passes through the sealing film, the movable structure is blocked outside the inflation groove by the sealing film, and the air hole is located in the inflation groove to connect the airbag and the inflation groove.

6. The anaerobic ammonium oxidation biofilm culture system according to claim 5, characterized in that: The movable structure includes an elastic member and a sliding block sleeved outside the load rod, one end of the elastic member is fixed to the load rod, and the other end is fixed to the sliding block, the sliding block is located on a side of the elastic member away from the cover plate, and the sliding block blocks the air hole; During the process of mounting the carrier assembly on the mounting base, the sliding block can slide relative to the load beam under the obstruction of the sealing film to squeeze the elastic member, thereby releasing the obstruction of the air hole.

7. The anaerobic ammonium oxidation biofilm culture system according to claim 6, characterized in that: A degassing groove is provided at the bottom of the packaging tube, and a limiting plate is provided at the opening of the degassing groove. A through hole is provided on the limiting plate to connect the interior of the degassing groove and the interior of the packaging tube. The carrier assembly can be installed in the packaging tube under the drive of the driving module, so that the load rod passes through the limiting plate, and the movable structure is blocked outside the degassing groove by the limiting plate. The air hole is located in the degassing groove to connect the degassing groove and the airbag.

8. The anaerobic ammonium oxidation biofilm culture system according to claim 1, characterized in that: The culture system further comprises a sewage conveying pipe, the inlet of the sewage conveying pipe is communicated with the liquid outlet, and the outlet of the conveying pipe is communicated with the storage tank.

9. The anaerobic ammonium oxidation biofilm culture system according to claim 1, characterized in that: The driving module includes a track frame and a clamp, wherein the track frame is fixed to the top wall of the nitrogen protection zone, and the clamp is movably connected to the track frame, and the clamp can move along the depth direction of the reactor to clamp or place the carrier assembly; And / or, the culture area is equipped with a fixing frame, the fixing frame is provided with a plurality of mounting holes, and the carrier assembly is passed through the mounting holes.

10. A method for cultivating an anaerobic ammonium oxidation biofilm, characterized in that: Using the anaerobic ammonium oxidation biofilm culture system according to any one of claims 1 to 9, the specific steps include: placing an uncultured carrier assembly on the reciprocating base in the storage tank, and driving the reciprocating base into the operation area; Start the driving module, clamp the carrier assembly in the operation area, move the carrier assembly and place the carrier assembly in the culture area; The target sewage is introduced into the culture area, nitrogen is introduced into the nitrogen protection area, and sewage is introduced into the storage tank; Start the driving module, clamp the carrier assembly that has been cultured in the culture area, move the carrier assembly and place it on the reciprocating base in the operation area, drive the reciprocating base to move to the storage pool, and take out the carrier assembly that has been cultured.

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