Method for inducing bee colony to improve propolis yield by utilizing multiple layers of gauze elements

Abstract

The invention relates to the technical field of bee product collection and processing, and discloses a method for inducing a bee colony to improve the propolis yield by using a multi-layer gauze element, and the method comprises the following steps: 1) constructing a bee colony with a colony potential of 4-12 frames, and arranging the bee colony in a standard beehive structure; (2) a food-grade multi-layer gauze structure is arranged at the top of the beehive, the mesh diameter of each layer of gauze is 0.8-1.2 mm, the gauze is stacked layer by layer, and the interlayer spacing is 6-10 mm; 3) adding a layer of new gauze between the lower layer and the upper layer when the proportion of the sealing area of the holes of the gauze on the lower layer reaches 80%-90%; and 4) repeatedly adding the gauze and prolonging the propolis collection period, and disassembling all the gauze to collect propolis after the target collection time is reached or the set number of layers is reached. By reasonably controlling the group potential and using a multi-layer gauze structure, the propolis collecting area is increased, the propolis yield is remarkably improved, and the problem of disordered or insufficient collection is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of bee product collection and processing, and specifically to a method for inducing bee colonies to increase the yield of propolis by using a multi-layer screen. Background Art

[0002] Propolis is a resin collected by bees from plant buds or tree trunks and processed by mixing it with the secretions of their supraorbital glands and wax glands into a fragrant, jelly-like solid substance. It is widely used in the fields of medicine, health products, cosmetics, etc. Currently, in the field of propolis production, there are already various methods for collecting propolis. For example, beehive propolis collectors, hive entrance propolis collectors, frame propolis collectors, grid propolis collectors, and new high-pressure polyethylene cover plate propolis collectors, etc. Among them, the most common and closest to the present invention is the traditional single-layer screen collection technology. This technology is widely used in many beekeeping farms, and its basic structure and operation method are as follows: At a suitable position on the top or inside of the beehive, a layer of ordinary screen is laid, which is an iron screen made of metal, and some also use ordinary plastic screens. Its function is to utilize the activity habits of bees in the beehive. When bees carry propolis into the beehive, they will leave part of the propolis on the screen when passing through it, thereby achieving the preliminary collection of propolis. For example, some beekeepers directly cover the inner cover of the beehive with an iron screen, and when bees move up and down, the propolis will adhere to the screen.

[0003] However, the traditional single-layer gauze net collection technology has significant drawbacks, mainly reflected in the following aspects: 1) Low yield: The single-layer gauze net provides a limited glue collection area, and there is insufficient space for bees to attach propolis. Bees only leave propolis during the short process of passing through the gauze net. Since there is only one layer of gauze net, the overall amount of collected propolis is small, and it does not have the function of attracting bees to collect. Structurally, it cannot make full use of the internal space of the beehive, and the propolis produced when bees move in most areas of the beehive cannot be effectively collected, resulting in an extremely low yield of propolis finally collected. This makes it difficult for beekeepers to meet the market demand for propolis output even with a large number of bee colonies, and the economic benefits are poor; 2) Poor quality: Most traditional single-layer gauze nets are made of iron gauze, and iron gauze is extremely prone to rust in the long-term humid beehive environment. The rusty iron gauze will react chemically with propolis, resulting in the pollution of propolis and excessive heavy metal content. Taking the iron gauze net as an example, iron ions in rust will mix into propolis, changing the original component structure of propolis, reducing the content of its active ingredients, and affecting the application of propolis in fields with extremely high quality requirements such as medicine and health products. Moreover, although ordinary plastic gauze nets do not have the problem of rusting, some plastic materials may release harmful substances, which also have an adverse impact on the quality of propolis; 3) Inconvenient collection: Since there is only one layer of gauze net, the distribution of propolis on the gauze net is relatively sparse and difficult to concentrate. When beekeepers collect propolis, they need to spend a lot of time and energy scraping propolis one by one on a large area of gauze net. The operation process is cumbersome and the labor intensity is high. From a structural perspective, the single-layer gauze net cannot promote the concentrated accumulation of propolis, increasing the collection difficulty and time cost, reducing the collection efficiency, and being unfavorable for large-scale propolis production. Summary of the Invention

[0004] In view of the deficiencies of the prior art, the present invention provides a method for using a multi-layer gauze net to induce bee colonies to increase the yield of propolis, which solves the problems of low yield, unstable quality, and cumbersome management in the process of propolis collection in the prior art.

[0005] To achieve the above objectives, the present invention is realized through the following technical solutions: A method for using a multi-layer gauze net to induce bee colonies to increase the yield of propolis, including the following steps:

[0006] 1) Construct a bee colony with a colony strength of 4-12 frames and place it in a standard beehive structure;

[0007] 2) Set up a food-grade multi-layer gauze net structure on the top of the beehive. The mesh diameter of each layer of gauze net is 0.8 mm - 1.2 mm, and the gauze nets are stacked layer by layer, with a layer spacing of 6 mm - 10 mm;

[0008] 3) When the proportion of the sealed glue area of the holes in the lower layer of gauze net reaches 80% - 90%, add a new layer of gauze net between the lower layer and the upper layer;

[0009] 4) Repeatedly add the screen and extend the propolis collection period. After reaching the target collection time or the set number of layers, remove all the screens to collect propolis.

[0010] Preferably, the number of layers of the multi-layer screen structure is 8 - 15 layers.

[0011] Preferably, the screen material is high-density polyethylene, polytetrafluoroethylene or polyether ether ketone, and the size of a single layer of screen is the same as that of the bee hive inner cover.

[0012] Preferably, the bee colony is controlled by a queen bee selected for high propolis-producing characteristics. The queen bee is replaced once a year and bred by artificial insemination or time-displaced mating. The ratio of the queen bee to drones is 1:100 - 1:150.

[0013] Preferably, the bee colony maintains the activity of propolis collection behavior through a seven-part feeding strategy, that is, during the non-nectar-flowing period, the colony's nutritional reserve is maintained at about 70% of the total requirement by quantitative feeding.

[0014] Preferably, the feed in the feeding is a liquid mixture, which includes, by mass percentage: 60% - 70% sugar source, 2% - 5% protein source, and 25% - 35% water. The sugar source is sucrose or fructose syrup, and the protein source is yeast powder or soybean powder.

[0015] Preferably, during the propolis collection process, after the collected screen is placed in an environment at -25 °C to -20 °C for 2 hours of freezing treatment, propolis is separated by mechanical knocking or manual peeling.

[0016] Preferably, the propolis is processed through purification to obtain finished propolis. The purification process includes: using ethanol or an ethanol-water mixture as a solvent, with a liquid-solid ratio of 5:1 - 8:1, an extraction temperature of 35 °C - 45 °C, an extraction time of 8 - 12 hours, repeating the extraction 2 - 3 times, and then obtaining the finished product through filtration, concentration and drying.

[0017] Preferably, the content of wax and other impurities ≤ 3%, and the content of insoluble substances ≤ 1%.

[0018] Preferably, the method further includes the step of enhancing the propolis secretion behavior by adjusting the bee hive environment. The adjustment includes: controlling the nest temperature between 32 °C and 35 °C, the relative humidity between 50% and 70%, the ventilation flow rate at 0.5 - 2 cubic meters per hour, and the light cycle between 12 - 14 hours per day.

[0019] The present invention provides a method for inducing bee colonies to increase propolis production by using a multi-layer screen. It has the following beneficial effects:

[0020] 1. By reasonably controlling the colony strength and adopting a multi-layer screen structure, the present invention increases the area where bees can attach propolis, significantly improves the propolis collection volume, and avoids the problems of collection chaos or insufficient collection ability caused by too large or too small colony strength.

[0021] 2. The present invention adopts the seven-part feeding method to increase the propolis content, promotes the enthusiasm of bees for collecting propolis, and at the same time reduces the wax secretion of worker bees, thereby improving the purity of propolis. The hygienic-grade plastic screen ensures the purity of propolis and avoids the risk of heavy metal pollution.

[0022] 3. The present invention simplifies the operation process, reduces the management difficulty, improves the propolis collection efficiency, standardizes the propolis collection process, makes management more convenient, and has high sustainability through the multi-layer screen propolis collection method. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a flowchart of the method steps of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0024] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0025] Please refer to the attached Figure 1 , an embodiment of the present invention provides a method for inducing bee colonies to increase propolis production using a multi-layer screen, including the following steps:

[0026] 1) Construct a bee colony with a colony strength of 4 - 12 frames and place it in a standard beehive structure;

[0027] During the process of constructing the bee colony, it is first necessary to reasonably determine the number and density of the colony strength. The optimization of the colony strength directly affects the secretion and plugging behavior of propolis. Through a mathematical model, the configuration of the bee colony is quantitatively calculated to ensure the reasonable colony strength and effective number of bees of the bee colony. The following mathematical model is used to describe the configuration and colony strength calculation of the bee colony.

[0028] Colony Strength Construction Model

[0029] The goal of constructing the bee colony is to determine the number of bees in the beehive. The formula is as follows:

[0030] N = F × H × D;

[0031] Where: N represents the total number of bees in the bee colony (unit: number of bees); F represents the number of honeycomb frames (unit: number of frames), F ∈ [4, 12]; H represents the number of bees carried by each frame (unit: number of bees), H ∈ [2000, 2500]; D represents the effective bee density coefficient, D ∈ [0.85, 0.95], which is used to describe the actual effective density of bees in each frame.

[0032] This formula is used to calculate the total number of bees in the bee colony. Adjust the number of frames, the number of bees in each frame, and the density coefficient according to the colony strength requirements to ensure the vitality and productivity of the bee colony in the actual breeding environment.

[0033] Colony strength overload and swarm control

[0034] When the total number of bees in the bee colony exceeds the maximum allowable density (N exceeds the threshold N max ), the bee colony needs to be divided. This threshold N max can be calculated by the following formula:

[0035] N max = F max × H max × D max ;

[0036] Where: F max is the maximum number of frames (unit: number of frames), set to 12; H max is the maximum number of bees per frame (unit: number of bees), set to 2500; D max is the maximum effective density coefficient, set to 0.95.

[0037] Therefore, the calculated maximum number of bees in the colony is:

[0038] N max = 12 × 2500 × 0.95 = 28,500 bees;

[0039] When the number of bees in the colony exceeds 28,500, a swarm operation is required. When swarming, the following steps are used to determine whether swarming is needed:

[0040] Observe the worker bee density of the bee colony. If the worker bee density of the bee colony exceeds 60% and there is a clustering phenomenon in the honeycomb frames in the colony, it is judged that the colony strength is overloaded;

[0041] Check the frequency of the colony's sealing behavior. If the frequency of its sealing behavior decreases significantly, it indicates that the colony is overcrowded and needs to be swarmed to maintain a stable sealing behavior.

[0042] Queen bee selection and genetic factor control

[0043] The selection of the queen bee has a decisive impact on the propolis sealing behavior of the bee colony, especially in high-yield propolis systems. To ensure the genetic superiority of the queen bee, the present invention adopts a comprehensive scoring model of three factors: propolis yield, propolis sealing area, and repeated sealing response ability:

[0044] P = α×S g +β×F p +γ×R c ;

[0045] In the formula: P represents the comprehensive score of the queen bee (standardized percentage score, unit: %); S g represents the annual propolis yield per single colony of the queen bee's ancestral generation (unit: g), S g ∈[300, 600]; F p represents the propolis sealing area index of the queen bee's colony (unit: cm2 / day), F p ∈[80, 200]; R c represents the scoring of the queen bee's repeated sealing response ability (dimensionless, value range: [0, 1]); α, β, γ are weighting factors, which are 0.4, 0.4, 0.2 respectively, and are used to adjust the influence weights of each factor on the queen bee's score.

[0046] Gene evaluation and artificial insemination process design during the queen bee breeding process ensure that its genetic genes have strong propolis sealing ability, thereby promoting the efficient collection of propolis. During artificial insemination, the ratio of the queen bee to the drone is controlled to be 1:100 to 1:150 to ensure stable propolis sealing behavior in the offspring population.

[0047] 2) Set a food-grade multi-layer screen structure on the top of the beehive. The mesh diameter of each layer of screen is 0.8 mm - 1.2 mm, and the screens are stacked layer by layer with a layer spacing of 6 mm - 10 mm;

[0048] Design of the multi-layer screen structure on the top of the beehive

[0049] The multi-layer screen structure includes multiple food-grade screen components. The screen component includes a screen mesh with pores, and the screen mesh is made of temperature-resistant and corrosion-resistant materials such as polyethylene, polytetrafluoroethylene, or polyether ether ketone. The pore diameter of each layer of screen is designed to be 0.8 - 1.2 mm, which can effectively guide bees to seal these pores. The size of each screen mesh is 480 mm × 380 mm, meeting the structural requirements of the beehive top cover and sub-cover.

[0050] The screen is connected between the top cover and the sub-cover of the beehive through a card slot structure, a guide rail module, or a clamping part to ensure the structural stability and adjustability. The spacing between each layer of screen and the upper layer is kept between 6 - 10 mm, providing enough space for bees to seal propolis.

[0051] Multi-layer screen induction mechanism

[0052] The setting of the multi-layer screen structure can not only provide a simulated gap-blocking environment for the bee colony, but also stimulate the propolis secretion behavior of the bee colony in the following ways:

[0053] Simulation of the motivation for blocking: Bees have a strong natural tendency to block. Especially when there is air circulation or physical cracks in the beehive, bees will actively collect propolis to block them. In this embodiment, the multi-layer screen imitates the natural gaps and air flow paths of the beehive, thereby inducing the bee colony to block these structural pores.

[0054] Dynamic blocking behavior: As each layer of screen pores is blocked, the bee colony will continue to block the pores of the upper layer, forming a continuous blocking cycle. The persistence of this blocking behavior can be maintained by inserting new screens layer by layer. When the blocking ratio of a layer of pores reaches 80% to 90%, a new screen layer is inserted above the original layer, thereby continuing to induce the bee colony to block and ensuring the continuous secretion of propolis.

[0055] Dynamic adjustment and maintenance of the multi-layer screen

[0056] In the beehive, the multi-layer screen structure can be dynamically adjusted according to the blocking progress of the bee colony. The specific adjustment methods are as follows:

[0057] First, according to the blocking behavior progress of the bee colony, monitor the blocking situation of the bee colony on each layer of the screen. If the blocking area of a certain layer of the screen reaches 80% to 90%, a new screen layer is gradually stacked into the current screen layer through a convenient insertion device. The number of layers stacked each time is controlled within 2 to 3 layers, and at most no more than 8 layers.

[0058] Second, with the change of external environmental conditions (such as temperature, humidity, air flow, etc.), it is necessary to adjust the number of screen layers and the spacing in a timely manner. For example, when the temperature is low or the external air flow is too strong, the blocking effect can be enhanced by increasing the number of screen layers; while when the blocking behavior of the bee colony is too slow, the number of screen layers can be reduced to relieve the blocking pressure on the bees.

[0059] Analysis of the adaptability of the bee colony to the multi-layer screen structure

[0060] Under the guidance of the multi-layer screen structure, the behavior of the bee colony gradually adapts to this artificially constructed "gap" environment. By blocking each pore layer by layer, bees not only enhance the stability of the beehive, but also effectively increase the secretion amount of propolis. In order to further optimize the blocking effect, this embodiment strengthens the adaptability of the bee colony in the following ways:

[0061] Behavior induction: In the initial adaptation stage of the bee colony, appropriately adding an appropriate amount of plant aroma or resinous liquid can enhance the interest and reaction speed of bees in screen blocking.

[0062] Time regulation: The time interval for inserting a new layer of screen mesh is 7 to 10 days each time, ensuring that the sealing behavior of the bee colony is not overly disturbed. At the same time, the response of the bee colony to the sealing of the mesh holes has a strong periodicity. By adjusting the number of inserted mesh layers and the time, the propolis production can be optimized.

[0063] Through the above technical means, the following technical effects can be achieved:

[0064] Significant increase in propolis production: The bee colony can maintain the sealing behavior for a long time, resulting in a substantial increase in propolis production. The propolis production per single colony per season reaches 250 to 1500 g.

[0065] Controllability of bee colony behavior: The multi-layer screen mesh structure can be dynamically adjusted according to the sealing progress of the bee colony, ensuring that the bee colony maintains an efficient sealing response in the long term.

[0066] Strong environmental adaptability: The multi-layer screen mesh design has strong adaptability and can be adjusted under different environmental conditions to maintain the efficient sealing of the bee colony.

[0067] 3) When the proportion of the sealed glue area of the holes in the lower-layer screen mesh reaches 80% - 90%, add a new layer of screen mesh between the lower layer and the upper layer;

[0068] Feeding strategy model and calculation relationship

[0069] This implementation method adopts the "seven-part feeding method". By adjusting the feeding frequency and feed composition, this method enables the bee colony to always maintain a moderate state of hunger, thereby stimulating it to secrete propolis to seal the gaps in the beehive structure. The basic principles of the feeding strategy are as follows:

[0070] Feeding time interval and frequency: Each feeding cycle is 7 to 10 days, ensuring that the feeding frequency is within a reasonable range. Frequent feeding will cause the bee colony to have excessive vitality and affect its propolis collection behavior, while an overly long starvation period will weaken the overall activity of the bees. Feeding amount and bee collection behavior: The amount of each feeding should be controlled within the appropriate energy requirement range of each group of bees.

[0071] Feed composition and enhancement of bee collection behavior

[0072] In order to enhance the propolis collection behavior of the bee colony, the proportion of the feed composition used is precisely designed to ensure that while the bees supplement nutrition, they remain highly active in secreting propolis. The composition of the feed includes the following main components:

[0073] Sucrose / fructose syrup (60 - 70%): Provides the main energy source for the bees and promotes them to maintain vitality;

[0074] Yeast powder or soybean powder (2 - 5%): Provides necessary proteins and trace elements to support the physiological needs of the bees for growth and propolis secretion;

[0075] Moisture (25–35%): Maintain the moisture balance of the bee colony and prevent over-thirst and over-hunger from affecting their activity.

[0076] This feed ratio remains stable during the feeding cycle to maintain the normal production state of the bee colony and promote its efficient secretion of propolis.

[0077] Analysis and regulation of the propolis collection behavior of the bee colony after feeding

[0078] After feeding, the propolis collection behavior of the bee colony will change due to the feed intake and its energy requirements. According to the adjustment of the feed, the propolis secretion behavior of the bees will be enhanced in the short term. Therefore, dynamic monitoring and regulation are required through the following steps:

[0079] First, monitor the activity and plugging behavior of the bee colony to determine whether it is in the optimal propolis collection state. The activity can be monitored in real time through the flight frequency of the bees and the activity density in the beehive.

[0080] Second, adjust the feeding amount and frequency of the next cycle based on the reaction of the bee colony (such as the increase or decrease in propolis secretion). When the plugging behavior of the bee colony reaches the expected plugging area (usually 80%–90%), the amount of the next feeding can be increased to maintain the continuity of the plugging behavior.

[0081] Finally, through image analysis or other data collection devices, the honey and propolis production level of the bee colony is evaluated in real time to form a data feedback loop. Through this process, the feeding strategy can be dynamically adjusted according to the actual results to achieve the best propolis yield.

[0082] Through the above feeding strategy, the following technical effects can be achieved:

[0083] Enhance the propolis collection behavior of the bee colony: A reasonable feeding strategy can significantly improve the propolis secretion level of the bee colony in the short term and steadily increase the propolis yield.

[0084] Regulate the vitality of the bee colony: Through moderate starvation regulation, stimulate the natural foraging behavior of the bees and avoid over-saturation and behavior degradation.

[0085] Dynamically optimize the propolis collection strategy: Combining the feedback data of the bee colony and behavior monitoring, the feeding strategy can be adjusted in a timely manner according to environmental changes and the reaction of the bee colony to ensure the continuous stability of the propolis yield.

[0086] 4) Repeat adding the screen mesh and extend the propolis collection cycle, and remove all the screen meshes to collect propolis after reaching the target collection time or the set number of layers;

[0087] In this embodiment, the process of propolis collection and preliminary screening is of great significance to the quality and purity of propolis. Through precise collection and screening operations, the efficient collection of propolis and the smooth progress of subsequent purification work can be ensured. This process includes four main steps: propolis collection, freezing, stripping, and screening, which are as follows:

[0088] Propolis collection operation

[0089] The propolis collection operation is carried out in the second stage after the multi-layer screen structure is blocked. First, the multi-layer screen structure of the blocking layer is removed by physical means. Each layer of the blocked screen has been filled with propolis secreted by bees for a long time to form a propolis layer. During this process, propolis covers each layer of the screen and adheres tightly.

[0090] After removing the blocking layer, the screen layer is placed in a cold storage or freezer, and the freezing time is controlled for about 2 hours. This freezing process helps the hardening of propolis, making it easier to peel off from the mesh holes.

[0091] Propolis stripping and collection

[0092] After freezing, the propolis is peeled off from the surface of the screen by means of light tapping or stripping. The specific operation steps are as follows:

[0093] Take out the frozen screen layer and place it on the workbench;

[0094] Use a rubber hammer to gently tap the edge of the screen, and the propolis will automatically fall off;

[0095] Or use a scraper to gently scrape along the surface of the screen, and the propolis will fall off in flakes or granules.

[0096] This method can effectively separate the propolis from the screen, avoiding the pollution and loss of propolis.

[0097] Preliminary screening and purification

[0098] After stripping, the propolis is preliminarily screened to remove foreign matters such as impurities, resins, and grass leaves. The preliminary screening operation is carried out through the following steps:

[0099] Coarse screening: Use a sieve with a pore size of 0.5 - 1.0 mm to roughly separate the propolis and remove larger impurity particles.

[0100] Fine screening: Put the propolis after coarse screening into a fine sieve with a mesh hole of 0.1 mm for further screening to remove tiny impurities.

[0101] Impurity separation: The propolis after screening is sent to an automatic purification device for secondary filtration to ensure that the purity meets the requirements.

[0102] This process can effectively remove non-propolis components in propolis, such as beeswax fragments, plant residues, etc., and improve the preliminary quality of propolis.

[0103] Collection and storage of propolis after primary screening

[0104] After primary screening, propolis can enter the storage container through the collection tank. The storage container should be made of materials that can prevent propolis from getting damp and deteriorating, such as stainless steel or glass containers. Propolis needs to be kept at low temperature and dry during storage to prevent it from mildewing or deteriorating due to high humidity.

[0105] During storage, the humidity of propolis should be kept below 6% to ensure its stability and good quality.

[0106] Through the above propolis collection and primary screening steps, the following technical effects can be achieved:

[0107] Efficient propolis separation: By combining freezing and scraping, the loss of propolis can be minimized to the greatest extent and the collection efficiency can be improved.

[0108] Improved propolis purity: The primary screening and purification processes effectively remove impurities, improving the preliminary purity of propolis and laying a foundation for subsequent purification.

[0109] Simple operation: This process collects and processes propolis by physical means without involving complex chemical reagents, ensuring the simplicity of operation and environmental friendliness.

[0110] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for inducing bee colonies to increase the propolis yield by using multiple layers of gauze, characterized in that, It includes the following steps: 1) Construct a bee colony with a colony strength of 4 - 12 frames and place it in a standard beehive structure; 2) Set up a food-grade multi-layer screen structure on the top of the beehive. The mesh diameter of each layer of the screen is 0.8 mm - 1.2 mm. The screens are stacked layer by layer, and the layer spacing is 6 mm - 10 mm; 3) When the proportion of the sealed glue area of the holes in the lower-layer screen reaches 80% - 90%, add a new layer of screen between the lower layer and the upper layer; 4) Repeat adding screens and extend the propolis collection period. After reaching the target collection time or the set number of layers, remove all the screens to collect propolis.

2. A method for inducing bee colonies to increase the propolis yield by using a multi-layer gauze, as claimed in claim 1, wherein The number of layers of the multi-layer screen structure is 8 - 15 layers.

3. A method for inducing bee colonies to increase the propolis yield by using multi-layered gauze nets according to claim 1, characterized in that, The screen material is high-density polyethylene, polytetrafluoroethylene or polyether ether ketone, and the size of a single layer of the screen is the same as that of the inner cover of the beehive.

4. A method for inducing a bee colony to increase the propolis yield by using a multi-layer gauze, as claimed in claim 1, wherein The bee colony is controlled by a queen bee selected for high propolis production characteristics. The queen bee is replaced once a year and is bred by artificial insemination or time-displaced mating. The ratio of the queen bee to the drone is 1:100 - 1:

150.

5. A method for inducing a bee colony to increase the propolis yield by using a multi-layer gauze, characterized in that, The bee colony maintains the activity of propolis collection behavior through a seven-point feeding strategy, that is, during the non-nectar-flow period, the group's nutritional reserve is maintained at about 70% of the total requirement by quantitative feeding.

6. A method for inducing a bee colony to increase the propolis yield by using a multi-layer gauze according to claim 5, characterized in that, In the feeding, the feed is a liquid mixture, which includes, by mass percentage: 60% - 70% of sugar source, 2% - 5% of protein source, and 25% - 35% of water. The sugar source is sucrose or fructose syrup, and the protein source is yeast powder or soybean powder.

7. A method for inducing bee colonies to increase the propolis yield by using a multi-layer gauze, as claimed in claim 1, wherein During the propolis collection process, after placing the collected screen in an environment with a temperature of -25 °C to -20 °C for freezing treatment for 2 hours, propolis separation is carried out by mechanical knocking or manual peeling.

8. A method for inducing a bee colony to increase the propolis yield by using a multi-layer gauze, as claimed in claim 1, wherein The propolis is obtained as a finished product after purification treatment. The purification process includes: using ethanol or an ethanol-water mixture as a solvent, with a liquid-solid ratio of 5:1 - 8:1, an extraction temperature of 35 °C - 45 °C, an extraction time of 8 hours - 12 hours, repeating the extraction 2 - 3 times, and then obtaining the finished product through filtration, concentration and drying.

9. A method for inducing a bee colony to increase the propolis yield by using a multi-layer gauze, characterized in that, The content of wax and other impurities ≤ 3%, and the content of insoluble substances ≤ 1%.

10. A method for inducing bee colonies to increase the propolis yield by using a multi-layer gauze, as claimed in claim 1, wherein The method also includes the step of enhancing the propolis secretion behavior by adjusting the beehive environment. The adjustment includes: controlling the nest temperature between 32 °C and 35 °C, the relative humidity between 50% and 70%, the ventilation flow rate at 0.5 cubic meters - 2 cubic meters per hour, and the light cycle between 12 hours and 14 hours per day.

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