Application of combined application of chitobiose and bacillus in promoting tomato growth, preventing and controlling tomato soil-borne bacterial wilt and relieving tomato acid stress

Abstract

The invention discloses application of combined application of chitobiose and bacillus in promoting tomato growth, preventing and controlling tomato soil-borne bacterial wilt and relieving tomato acid stress. The chitobiose and the bacillus amyloliquefaciens SQR9 are combined to promote the growth of the tomatoes, relieve the acid stress of the tomatoes and improve the tomato fruit quality. The chitobiose combined bacillus amyloliquefaciens SQR9 is applied to prevention and control of tomato soil-borne bacterial wilt and enhancement of tomato system resistance. Chitosan disaccharide can promote the growth of bacillus amyloliquefaciens, and also can significantly improve the inhibition ability of bacillus amyloliquefaciens to ralstonia solanacearum. In a greenhouse pot experiment, the chitobiose can improve the biomass of tomatoes and promote the growth and development of root systems. Chitosan disaccharide and bacillus amyloliquefaciens are simultaneously applied into soil by a root irrigation method, so that the disease index of tomato soil-borne bacterial wilt can be reduced, the activity of resistant enzyme of a plant system can be increased, the acid stress of tomatoes can be relieved, the biomass of the tomatoes can be increased, and the quality of tomato fruits can be improved.

Description

Technical Field

[0001] The present invention belongs to the field of biological fertilizers and pesticides, and relates to the application of chitobiose in promoting the growth of tomatoes, alleviating acid stress, and improving fruit quality. Background Art

[0002] Chitin is a polysaccharide polymerized by N-acetylglucosamine through β-1,4 glycosidic bonds, and is the second largest macromolecular renewable biomass resource in nature after cellulose. Chitin widely exists in organisms, mainly derived from the shells of crustaceans such as shrimps and crabs, the organs of mollusks, fungal cell walls, diatoms, and arthropods. Chitin has high biocompatibility and safety. Chitin is insoluble in water, and the smaller its molecular weight, the easier it is to be absorbed and utilized by plants, and the more obvious the effect on plants.

[0003] Chitosan oligosaccharide is a product obtained by deacetylating chitin and then performing acid hydrolysis or enzymatic hydrolysis. Its degree of polymerization is 2-20, with a small molecular weight and is easily absorbed by plants. Chitosan oligosaccharide has biological functions such as regulating plant growth, development, and inducing plant resistance, and is an internationally recognized immune inducer. Studies have shown that foliar spraying of chitosan oligosaccharide has different control effects on various plant pathogens such as Phytophthora capsici, Fusarium wilt of cotton, and early blight of tomatoes. The main reason is that it can stimulate the systemic resistance of the above-ground part of plants and inhibit plant leaf pathogens. However, it is not known whether chitosan oligosaccharide can prevent and control plant soil-borne diseases, and different molecular weights have different antibacterial modes of action. Currently, chitosan oligosaccharides on the market are all mixtures, and there is little research on the role of chitobiose.

[0004] Plant growth promoting rhizobacteria (PGPR) are a type of biocontrol microorganisms that attach to the rhizosphere of plants and can promote the colonization of beneficial microorganisms, promote plant growth, and inhibit the growth of harmful microorganisms. Bacillus amyloliquefaciens is a typical rhizosphere growth promoting bacterium and can have a certain biocontrol effect on various pathogens. Bacillus has extremely strong survival ability and can survive in the form of spores under extreme conditions, playing an important role in agricultural production.

[0005] Tomato bacterial wilt is a devastating soil-borne disease caused by Ralstonia solanacearum (abbreviated as bacterial wilt), which can cause irreversible wilting and death of crops, resulting in serious crop yield reduction. Studies have shown that the combination of chitosan oligosaccharide and actinomycete XW5 shows a better inhibitory effect on apple rot than the single application of XW5 [1]However, there is currently little research on the interaction between chitooligosaccharides and Bacillus spp., and most of the research focuses on the inhibitory effect of chitooligosaccharides alone on pathogenic bacteria. There is less research on chitobiose, and it remains to be further explored whether the interaction between chitobiose and Bacillus spp. can reduce the occurrence of soil-borne bacterial wilt, as well as the concentration of chitobiose required for its effect. Summary of the Invention

[0006] The object of the present invention is to provide the application of chitobiose in promoting the growth of tomatoes, alleviating acid stress, and improving the fruit quality in view of the above deficiencies of the prior art.

[0007] Another object of the present invention is to provide the application of chitobiose combined with SQR9 in preventing and controlling soil-borne bacterial wilt of tomatoes and enhancing the systemic resistance of tomatoes.

[0008] The object of the present invention can be achieved by the following technical solutions:

[0009] The application of chitobiose in promoting the growth of tomatoes, alleviating the acid stress of tomatoes, and improving the fruit quality of tomatoes.

[0010] As a preferred embodiment of the present invention, chitobiose is used by root irrigation.

[0011] As a preferred embodiment of the present invention, the application amount of chitobiose is 0.025 - 15 mg / plant.

[0012] The application of chitobiose combined with Bacillus amyloliquefaciens SQR9 with the preservation number of CGMCC NO.5808 in preventing and controlling soil-borne bacterial wilt of tomatoes and enhancing the systemic resistance of tomatoes. Bacillus amyloliquefaciens SQR9 with the preservation number of CGMCC NO.5808 is disclosed in CN113980855A.

[0013] As a preferred embodiment of the present invention, chitobiose can significantly promote the increase in the biomass of the said Bacillus amyloliquefaciens SQR9.

[0014] As a preferred embodiment of the present invention, chitobiose combined with Bacillus amyloliquefaciens SQR9 with the preservation number of CGMCC NO.5808 significantly reduces the disease index of soil-borne bacterial wilt of tomatoes and reduces the incidence of tomato bacterial wilt.

[0015] As a preferred embodiment of the present invention, the enhancement of the systemic resistance of tomatoes is manifested as an increase in the activities of superoxide dismutase, peroxidase, and polyphenol oxidase on the tomato leaf surface.

[0016] Application of chitobiose in combination with Bacillus amyloliquefaciens SQR9 with preservation number CGMCC NO.5808 in alleviating acid stress of tomatoes, increasing tomato biomass, and improving tomato fruit quality.

[0017] Application of chitobiose in preparing a product for promoting the growth of tomatoes, alleviating acid stress of tomatoes, and improving tomato fruit quality.

[0018] Application of the microbial agent of chitobiose and Bacillus amyloliquefaciens SQR9 with preservation number CGMCC NO.5808 in preparing a product with any one or more of the following uses:

[0019] (1) Alleviating acid stress of tomatoes,

[0020] (2) Increasing tomato biomass,

[0021] (3) Improving tomato fruit quality,

[0022] (4) Preventing and controlling tomato soil-borne bacterial wilt,

[0023] (5) Enhancing tomato systemic resistance.

[0024] Beneficial effects:

[0025] It has been found through research that in the indoor microplate system, chitobiose can promote the growth of Bacillus amyloliquefaciens and significantly improve the inhibitory ability of Bacillus amyloliquefaciens against Ralstonia solanacearum; in the greenhouse pot experiment, chitobiose can increase tomato biomass and promote root growth and development. When chitobiose and Bacillus amyloliquefaciens are simultaneously applied to the soil by irrigation, it can reduce the disease index of tomato soil-borne bacterial wilt and increase the activity of plant systemic resistance enzymes. The combined application of chitobiose and SQR9 can also alleviate the acid stress of tomatoes, increase tomato biomass, and improve tomato fruit quality. Description of the drawings

[0026] Figure 1 Effect of chitobiose on tomato growth

[0027] Figure 2 Effect of different treatments on the disease index of tomato soil-borne bacterial wilt

[0028] Figure 3 Effect of chitobiose on tomato growth under acid stress

[0029] Figure 4 Effect of combined application of chitobiose and SQR9 on tomato growth under acid stress Detailed implementation manners

[0030] Example 1 Investigation of the growth-promoting effect of chitobiose on tomatoes through pot experiments

[0031] Tomato variety: Hong Aisheng tomato

[0032] Tomato seeds were soaked in 5% sodium hypochlorite (NaClO) solution for 5 min and rinsed several times with sterile water. In a laminar flow hood, the disinfected tomato seeds were placed in a sterile petri dish lined with filter paper moistened with sterile water, spread evenly, and germinated in an incubator at 30 °C for 48 h. Tomato seedlings with consistent growth were selected and transplanted into a 72-well seedling tray and cultured in a greenhouse. Two weeks after seeding, tomato seedlings with consistent growth were selected and transplanted into a six-well seedling tray and cultured for another week. A total of five concentrations of chitosan oligosaccharide, namely 0.025 mg / plant, 0.5 mg / plant, 1.5 mg / plant, 4.5 mg / plant, and 15 mg / plant, and a blank control were designed for the experiment and applied by the root irrigation method. Three weeks after tomato transplantation, samples were collected to measure the fresh weight of the above-ground part, fresh weight of the underground part, plant height, and root length of the tomato.

[0033] The results are shown in Figure 1 Table 1. Compared with the control group, the application of different concentrations of chitosan oligosaccharide promoted the growth of plants and significantly increased the fresh weight of the above-ground part, fresh weight of the underground part, plant height, and root length of the measured tomatoes.

[0034] Table 1 Effects of chitosan oligosaccharide on tomato growth

[0035]

[0036] Note: Different letters in the same column of the table indicate a significant level of 0.05, and the same applies hereinafter.

[0037] Example 2 Chitosan oligosaccharide has a promoting effect on SQR9

[0038] NA liquid medium: 10.0 g of glucose, 5.0 g of peptone, 10.0 g of yeast extract, 1000 ml of distilled water, adjust the pH to 7.2 - 7.4, and autoclave at 121 °C for 20 min.

[0039] NA solid medium is NA liquid medium added with 2 - 3% (w / w) agar.

[0040] Basal medium: 13.24 g of disodium hydrogen phosphate dodecahydrate, 0.12 g of anhydrous magnesium sulfate, 1.6 g of potassium dihydrogen phosphate, 7 g of ammonium sulfate, 0.5 g of diammonium hydrogen citrate, 8 ml of 50% glycerol, make up to 1 L with ultrapure water, and autoclave at 121 °C for 20 min.

[0041] The strain SQR9, with the preservation number of CGMCC NO.5808, is an existing publicly disclosed strain.

[0042] Streak the glycerol-preserved SQR9 on NA solid medium and incubate at 30 °C for 8 h; pick a single colony on the plate and transfer it to NA liquid medium, and incubate overnight at 30 °C and 170 rpm. Take 6000 rpm of fresh bacterial liquid and centrifuge at room temperature for 5 min, collect the cells, wash the cells 3 times with sterile water to remove the residual medium on the surface of the strain, and adjust the concentration of the bacterial liquid to 1×10 8 CFU / mL to obtain a bacterial suspension for use.

[0043] A total of five concentrations of chitobiose, namely 0.1 mg / ml, 0.2 mg / ml, 0.3 mg / ml, 0.4 mg / ml, 0.5 mg / ml, and a blank control, were designed for the experiment. The medium was 20% NA medium (dilute the NA liquid medium 5 times with sterile water), and the inoculation amount of SQR9 was 1% (final concentration 1×10 6 CFU / mL), shake culture at 30 °C and 170 rpm for 48 h, measure OD600 on an enzyme-labeled instrument, and examine the growth of SOR9.

[0044] The promoting effect of chitobiose on the growth of SQR9 is shown in Table 2. The results show that compared with the control group, chitobiose can significantly promote the increase in the biomass of Bacillus amyloliquefaciens SQR9 (P<0.05).

[0045] Table 2 Effects of chitobiose on the biomass and growth rate of SQR9

[0046]

[0047] Example 3 Chitobiose enhances the effect of SQR9 in inhibiting soil-borne Ralstonia solanacearum

[0048] Streak the Ralstonia solanacearum strain Rs1115-RFP labeled with red fluorescent protein on an NA solid medium plate supplemented with 30 μg / mL gentamicin, place the plate at 30 °C and incubate until single colonies appear, pick a single colony and transfer it to NA liquid medium for overnight culture, take 6000 rpm of fresh bacterial liquid and centrifuge at room temperature for 5 min, collect the cells, wash the cells 3 times with sterile water to remove the residual medium on the surface of the strain, and adjust the concentration of the bacterial liquid to 1×10 8 CFU / mL to obtain a bacterial suspension for use.

[0049] Streak the glycerol-preserved SQR9 on NA solid medium and incubate at 30 °C for 8 h; pick a single colony on the plate and transfer it to NA liquid medium, and incubate overnight at 30 °C and 170 rpm. Take 6000 rpm of fresh bacterial liquid and centrifuge at room temperature for 5 min, collect the cells, wash the cells 3 times with sterile water to remove the residual medium on the surface of the strain, and adjust the concentration of the bacterial liquid to 1×10 8 CFU / mL to obtain a bacterial suspension for use.

[0050] A total of five concentrations of chitobiose, namely 0.1 mg / ml, 0.15 mg / ml, 0.2 mg / ml, 0.25 mg / ml, and 0.3 mg / ml, and a blank control were designed for the experiment, with a total of 6 treatments. The medium was 20% NA medium (the NA liquid medium was diluted 4 times with sterile water). The inoculation amounts of SQR9 and Ralstonia solanacearum Rs1115-RFP were both 1%. The cultures were shaken at 30 °C and 170 rpm for 48 h. The OD600 and the red fluorescence intensity RFP (emission light: 587 nm, absorption light: 610 nm) were measured using an ELISA reader, and the relative growth of Ralstonia solanacearum was calculated as: log10(RFP / OD 600 ).

[0051] The results are shown in Table 3. Compared with the control group, chitobiose could significantly enhance the ability of SQR9 to inhibit Ralstonia solanacearum (P<0.05).

[0052] Table 3 Effects of chitobiose on the inhibition of Ralstonia solanacearum by SQR9

[0053]

[0054] Example 4 The growth-promoting effect of the combined application of chitobiose and SQR9 on tomatoes was investigated through a pot experiment

[0055] For the pot experiment, the tomato variety, tomato seedling raising, and transplantation were the same as in Example 1. A total of 4 treatments were designed: 1) SQR9 group: One week after the seedling transplantation, SQR9 was evenly poured into the tomato roots, and the final concentration of SQR9 was 10 7 CFU / g soil. 2) Chitobiose 4 mg / plant × 2 groups: One week after the seedling transplantation, 4 mg of chitobiose was evenly poured into the tomato roots, and 4 mg of chitobiose was poured again one week later. 3) SQR9 + chitobiose 4 mg / plant × 2 groups: One week after the seedling transplantation, SQR9 and 4 mg of chitobiose were evenly poured into the tomato roots, and 4 mg of chitobiose was poured again one week later. The final concentration of SQR9 was 10 7 CFU / g soil. 4) CK group, with an equal amount of clear water added as a blank control. Three weeks after the tomato transplantation, samples were taken to measure the fresh weight of the aboveground part, the fresh weight of the underground part, the dry weight of the aboveground part, and the dry weight of the underground part of the tomatoes

[0056] The results are shown in Table 4. Compared with the control group, the combined application of chitobiose and SQR9 could promote the growth and development of tomatoes. The fresh aboveground biomass and fresh underground biomass with the combined addition of SQR9 and chitobiose increased significantly by 62.62% and 98.45% respectively, and the dry aboveground biomass and dry underground biomass increased significantly by 66.96% and 216.25% respectively. The combined treatment of chitobiose and SQR9 had the best effect

[0057] Table 4 Effects of the combined application of chitobiose and SQR9 on tomato growth

[0058]

[0059] Example 5 Chitobiose enhanced the potted effect of SQR9 in inhibiting soil-borne Ralstonia solanacearum.

[0060] For the potted experiment, the tomato variety, tomato seedling raising, and transplanting were the same as in Example 1. A total of 5 treatments were designed for the experiment: 1) SQR9 group: One week after the seedling transplantation, SQR9 and Ralstonia solanacearum were evenly irrigated into the tomato roots. The final concentration of SQR9 was 10 7 CFU / g soil, and the final concentration of Ralstonia solanacearum was 10 6 CFU / g soil. 2) SQR9 + chitobiose 2 mg / plant × 2 groups: One week after the seedling transplantation, SQR9, Ralstonia solanacearum, and 2 mg of chitobiose were evenly irrigated into the tomato roots. After one week, another 2 mg of chitobiose was irrigated. The final concentration of SQR9 was 10 7 CFU / g soil, and the final concentration of Ralstonia solanacearum was 10 6 CFU / g soil. 3) SQR9 + chitobiose 4 mg / plant × 2 groups: One week after the seedling transplantation, SQR9, Ralstonia solanacearum, and 4 mg of chitobiose were evenly irrigated into the tomato roots. After one week, another 4 mg of chitobiose was irrigated. The final concentration of SQR9 was 10 7 CFU / g soil, and the final concentration of Ralstonia solanacearum was 10 6 CFU / g soil. 4) SQR9 + chitobiose 6 mg / plant × 2 groups: One week after the seedling transplantation, SQR9, Ralstonia solanacearum, and 6 mg of chitobiose were evenly irrigated into the tomato roots. After one week, another 6 mg of chitobiose was irrigated. The final concentration of SQR9 was 10 7 CFU / g soil, and the final concentration of Ralstonia solanacearum was 10 6 CFU / g soil. 5) Ralstonia solanacearum group: One week after the seedling transplantation, Ralstonia solanacearum was evenly irrigated into the tomato roots. The final concentration of Ralstonia solanacearum was 10 6 CFU / g soil. Once the disease occurred after inoculating Ralstonia solanacearum, the record began.

[0061] The disease grades of the plants were divided into 5 levels [2] : 0 = no disease symptoms, 1 = 1% - 25% of the plant parts showed disease symptoms, 2 = 26% - 50% of the plant parts showed disease symptoms, 3 = 51% - 75% of the plant parts showed disease symptoms, 4 = 76% - 100% of the plant parts showed disease symptoms or the plant had died. The disease index was used to characterize the disease situation, and the calculation formula was: Plant disease index = [∑(number of diseased plants at each level × corresponding level) / (total number of investigated plants × highest level value)].

[0062] The results were as Figure 2 shown in Table 5, and the results indicated that after adding chitobiose, the disease index of tomato soil-borne Ralstonia solanacearum could be significantly reduced, and the disease severity of Ralstonia solanacearum was decreased.

[0063] Table 5 Control Effects of Different Treatments on Tomato Soil-borne Bacterial Wilt

[0064]

[0065] Example 6 Chitosan Oligosaccharide Enhances Plant Systemic Resistance

[0066] The pot experiment, including tomato variety, tomato seedling raising, transplanting, and the application method of chitosan oligosaccharide, was the same as in Example 5. Leaf samples were taken 5 days after the application of chitosan oligosaccharide. Finally, superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO) were detected using the corresponding enzyme activity detection kits (for plants) purchased from Grace Biotechnology.

[0067] The results are shown in Table 6. Chitosan oligosaccharide applied by root irrigation can significantly increase the activities of SOD, POD, and PPO in tomato leaves, indicating that the application of chitosan oligosaccharide has a significant promoting effect on the resistance enzyme activities of tomato leaves, can enhance the systemic resistance of tomatoes, and helps tomatoes resist the infection of bacterial wilt.

[0068] Table 6. Effects of Chitosan Oligosaccharide on the Resistance Enzyme Activities of Tomato Leaves

[0069]

[0070]

[0071] Example 7 Chitosan Oligosaccharide Alleviates Acid Stress in Tomatoes

[0072] Test the nutrient status of acidic soil: pH value is 4.33, and the organic matter content is 5.23 g / kg.

[0073] The tomato variety, tomato seedling raising, and transplanting were the same as in Example 1. One week after transplanting, the following treatments were designed for the experiment: 1) Chitosan oligosaccharide 2.5 mg / plant × 2 groups: 2.5 mg of chitosan oligosaccharide was evenly applied to the roots by root irrigation, and 2.5 mg was applied again one week later. 2) Chitosan oligosaccharide 5 mg / plant × 2 groups: 5 mg of chitosan oligosaccharide was applied by root irrigation, and 5 mg was applied again one week later. 3) Chitosan oligosaccharide 7.5 mg / plant × 2 groups: 7.5 mg of chitosan oligosaccharide was applied by root irrigation, and 7.5 mg was applied again one week later. 4) Blank control: Equal volume of clear water was used instead of chitosan oligosaccharide.

[0074] Control the temperature at 25 - 35 °C, the light time at 12 h, and the humidity at 60 - 80% RH, and observe the growth status of tomatoes. The results are shown in Figure 3 and Table 7. Chitosan oligosaccharide applied by root irrigation can significantly alleviate the acid stress in tomatoes.

[0075] As can be seen from Table 7, under acid stress, the growth of tomatoes is severely inhibited. Adding an appropriate amount of chitosan oligosaccharide can significantly improve the growth of plants. Among them, compared with the control group, Treatment 3 can double the above-ground and underground biomass accumulation of tomatoes.

[0076] Table 7 Effects of chitobiose on the growth of tomatoes under acid stress

[0077]

[0078]

[0079] Table 8 Effects of chitobiose on the fruit quality of tomatoes under acid stress. Chitobiose had no significant effect on the soluble sugar of tomatoes under acid stress. The contents of soluble protein, vitamin C and nitrate increased with the increase of chitobiose concentration, and the maximum increases were 92.8%, 215.4% and 84.89% respectively. Applying an appropriate amount of chitobiose could significantly increase the contents of soluble protein, vitamin C and nitrate in tomatoes and improve their quality.

[0080] Table 8 Effects of chitobiose on the fruit quality of tomatoes under acid stress

[0081]

[0082] Example 8 Mitigation of acid stress in tomatoes by combined application of chitobiose and SQR9

[0083] The acid soil, tomato variety, tomato seedling raising and transplanting were the same as in Example 7. One week after transplanting, the following treatments were designed for the experiment: 1) One week after transplanting the seedlings, SQR9 was evenly poured into the roots of tomatoes, and the final concentration of SQR9 was 10 7 CFU / g soil. 2) Chitobiose 4 mg / plant × 2 groups: One week after transplanting the seedlings, 4 mg of chitobiose was applied by root irrigation, and 4 mg was applied again one week later. 3) SQR9 + chitobiose 4 mg / plant × 2 groups: One week after transplanting the seedlings, 4 mg of chitobiose and SQR9 were applied by root irrigation, and the final concentration of SQR9 was 10 7 CFU / g soil, and 4 mg of chitobiose was applied again one week later. 4) Blank control: An equal volume of clear water was used instead of chitobiose.

[0084] The temperature was controlled at 25 - 35°C, the light duration was 12 h, and the humidity was 60 - 80% RH. The growth status of tomatoes was observed. The results are shown in Figure 4 and Table 9. Root irrigation with chitobiose could significantly alleviate the acid stress of tomatoes.

[0085] As can be seen from Table 9, significant differences were observed in both the aboveground and underground parts when chitobiose, chitobiose and SQR9 were co-treated. The fresh weight of the aboveground part increased by 14.02%, 72.42% and 74.25% respectively, and the fresh weight of the underground part increased by 16.58%, 64.85% and 148.01% respectively. Compared with the control, the co-treatment of SQR9, chitobiose, and chitobiose and SQR9 increased the dry weight of the aboveground part by 14.53%, 80.14% and 107.09% respectively, and the dry weight of the underground part by 40.00%, 105.72% and 201.43% respectively. This indicates that the addition of SQR9 and chitobiose can promote the synthesis of dry matter in tomato seedlings during the seedling stage. The biomass of tomatoes reached the highest level in the treatment with the combined application of SQR9 and chitobiose, which was significantly higher than that of other treatments. The second was the case of single inoculation with chitobiose.

[0086] Table 9 Effects of combined application of chitobiose and SQR9 on tomato growth parameters under acid stress

[0087]

[0088] As can be seen from Table 10, compared with CK, the number of fruits per plant and the yield per plant of the co-treatments of SQR9, chitobiose, and chitobiose and SQR9 were all increased. However, the weight of single fruit in the SQR9 and chitobiose treatment groups was lower than that of CK. The co-treatment group of chitobiose and SQR9 had the most significant improvement effect. The number of fruits per plant and the yield per plant increased by 119.31% and 120.08% respectively compared with CK.

[0089] Table 10 Effects of combined application of chitobiose and SQR9 on tomato fruit yield under acid stress

[0090]

[0091]

[0092] As can be seen from Table 11, compared with CK, the soluble solids, soluble proteins, vitamin C and soluble sugars of the co-treatments of SQR9, chitobiose, and chitobiose and SQR9 were all increased. The co-treatment group of chitobiose and SQR9 had the most significant improvement effect. The soluble solids, soluble proteins, vitamin C and soluble sugars increased by 16.00%, 81.62%, 79.32% and 32.67% respectively compared with CK.

[0093] Table 11 Effects of combined application of chitobiose and SQR9 on tomato fruit quality under acid stress

[0094]

[0095] References

[0096] [1] Zhang Mengxin, Xue Shengping. Preliminary exploration on the combination of potassium fulvate and chitosan oligosaccharide with antagonistic bacterium XW5 to inhibit apple rot disease [J]. Agriculture and Technology, 2020, 40(09): 20-23.

[0097] [2] Kempe, J., and L. Sequeira. Biological control of bacterial wilt of potatoes: attempts to induce resistance by treating tubers with bacteria. Plant disease, 1983, 67: 499–503.

Claims

1. Application of chitobiose in promoting the growth of tomatoes, alleviating acid stress of tomatoes, and improving the quality of tomato fruits.

2. The application according to claim 1, wherein Chitobiose is used by root irrigation.

3. The application according to claim 2, wherein The application amount of chitobiose is 2.5 - 7.5 mg / plant.

4. Application of chitobiose combined with Bacillus amyloliguefaciens SQR9 with the preservation number of CGMCC NO.5808 in preventing and controlling tomato soil-borne bacterial wilt and enhancing the systemic resistance of tomatoes.

5. The application according to claim 4, characterized in that, Chitobiose can significantly promote the increase in the biomass of Bacillus amyloliguefaciens SQR9.

6. The application according to claim 4, characterized in that, Chitobiose combined with Bacillus amyloliguefaciens SQR9 with the preservation number of CGMCC NO.5808 significantly reduces the disease index of tomato soil-borne bacterial wilt and reduces the incidence of tomato bacterial wilt.

7. The application according to claim 4, characterized in that The enhancement of the systemic resistance of tomatoes mentioned above is manifested as an increase in the activities of superoxide dismutase, peroxidase, and polyphenol oxidase on the tomato leaf surface.

8. Application of chitobiose combined with Bacillus amyloliguefaciens SQR9 with the preservation number of CGMCC NO.5808 in alleviating acid stress of tomatoes, increasing tomato biomass, and improving the quality of tomato fruits.

9. Application of chitobiose in preparing a product for promoting the growth of tomatoes, alleviating acid stress of tomatoes, and improving the quality of tomato fruits.

10. Application of the microbial agent of chitobiose and Bacillus amyloliguefaciens SQR9 with the preservation number of CGMCC NO.5808 in preparing a product with any one or more of the following uses: (1) Alleviating acid stress of tomatoes, (2) Increasing tomato biomass, (3) Improving the quality of tomato fruits, (4) Preventing and controlling tomato soil-borne bacterial wilt, (5) Enhancing the systemic resistance of tomatoes.

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