Synergistic compound composition containing two active components and application

Abstract

The invention belongs to the field of pesticides, and relates to a synergistic compound composition containing two active components. The composition comprises an active component A and an active component B, the component A is selected from piperic acid derivative compounds with the following structure, and the component B is selected from pyridaben; the weight part ratio of the component A to the component B is (1: 50)-(50: 1); the composition disclosed by the invention has an obvious synergistic effect and can be used for preventing and treating various pests and pest mites. # imgabs0 #

Description

[0001] This application is a divisional application of a Chinese invention patent application. The original application date was November 29, 2022, the application number was 202211512077.8, the invention title was: A synergistic compound composition of two active ingredients and its application, and the publication number was: CN118104669A; due to the lack of unity in the original application, the applicant filed a divisional application. Technical Field

[0002] The present invention belongs to the field of pesticides and relates to a synergistic compound composition of two active ingredients and its application. Background Art

[0003] Agents used to control mite damage are collectively called acaricides. Mites have the characteristics of small size, fast reproduction, short generation cycle, strong adaptability, and easy development of drug resistance. They are one of the pest populations that are difficult to control on fruit trees, vegetables, ornamental plants, field crops and other crops. Applying chemical acaricides is the most effective means to control harmful mites. However, long-term continuous high-dose application of a single chemical acaricide easily causes a series of problems such as the generation of drug resistance in harmful mites, the residue of the agent, and environmental pollution. Rational compounding or mixing of chemical insecticides and acaricides has positive characteristics such as expanding the insecticidal and acaricidal spectrum, improving the control effect, extending the suitable application period of pesticides, reducing the dosage of pesticides, reducing phytotoxicity, reducing residues, and delaying the occurrence and development of drug resistance and resistance in pests and mites. Compounding or mixing insecticides and acaricides is one of the most effective methods to solve the above problems. The price of developing new insecticides is rising continuously. In contrast, the development and research of highly efficient, low-toxic, and low-residue compounding and mixing have received attention at home and abroad due to less investment and short research and development cycles, and the development and research efforts have been increased.

[0004] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application. Among them, compound I-72 has contact and stomach toxicity activities and good conduction activity, and can be used to control various pests such as Lepidoptera, Hemiptera, Thysanoptera, Coleoptera, etc. The structural formula of this compound is as follows:

[0005]

[0006] Compounding different varieties of ingredients may produce some unexpected technical effects and is also one of the effective methods to control resistant pests. However, so far, there has been no report on the compound composition of compound I-72 and acaricides. Summary of the Invention

[0007] The purpose of the present invention is to provide a synergistic compound composition of two active ingredients and its application.

[0008] The technical solution adopted by the present invention to achieve the above purpose is as follows:

[0009] A synergistic compound composition of two active components, the composition being two active components A and B. Component A is selected from piperic acid derivative compounds of the following structures (i.e., compound I-72), and component B is selected from acaricides; the weight ratio of the two active components A and B is 1:50 to 50:1; wherein, the structural formula of the piperic acid derivative compound is as follows:

[0010]

[0011] The component B is a miticide selected from pyridaben, cyetpyrafen, fluacrypyrim, fenpyroximate, tebufenpyrad, cyenopyrafen, etoxazole, hexythiazox, clofentezine, propargite, bifenazate, cyflumetofen, azocyclotin, cyhexatin, fenbutatin oxide, phenproxide, benzoximate, triarathene, dofenapyn, halfenprox, chloropropylate, dienochlor, flubenzimine, tazimcarb, phostin, dicofol, tetradifon, chlorfenethol, tetrasul, tetranactin, medimeform, aramite, chlorfenson, chlorfensulphide, genite, MNFA, cycloprate, amidoflumet, fenazaflor, clenpirin, thioquinox, fenazaquin, binapacryl, fluenetil, dinocton, chlorbenside, cymiazole, morphothion, pyriminostrobin, pyrimidifen, acequinocyl, chinomethionat, chloromebuform, malonoben, bromocyclen, azobenzene, TEPP, dinosulfon, bromopropylate,Dinopenton, dinoterbon, prothidathion, dinex, dinobuton, chlorobenzilate, benoxafos, endosulfan, sulfur, DNOC, semiamitraz, amitraz, chlordimeform, formetanate, Flipper (biological acaricide containing C7-C20 fatty acids), pyflubumide, acynonapyr or trifluenfuronate.

[0012] Preferably, component A in the composition is selected from the above-mentioned piperic acid derivative compounds; component B is selected from pyridaben, cyetpyrafen, fluacrypyrim, fenpyroximate, tebufenpyrad, cyenopyrafen, etoxazole, hexythiazox, clofentezine, propargite, bifenazate, cyflumetofen, azocyclotin, pyriminostrobin, pyrimidifen, acequinocyl, bromopropylate, pyflubumide, acynonapyr, trifluenfuronate;

[0013] The weight ratio of the two active components A and B is 1:20 to 20:1.

[0014] More preferably, component A in the composition is selected from the above-mentioned piperic acid derivative compounds; component B is selected from pyridaben, cyetpyrafen;

[0015] The weight ratio of the two active components A and B is 1:10 to 10:1.

[0016] Application of the composition, the composition is used for preparing insecticidal and acaricidal drugs for controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, and evergreen trees.

[0017] Use of the composition for preventing or controlling pests, their larvae and eggs of Acarina, Lepidoptera, Hemiptera, Homoptera, Coleoptera, Diptera, Thysanoptera.

[0018] The synergistic compound composition of the present invention has an obvious synergistic effect on controlling pests and mite pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees. The results of indoor bioactivity assays show that mixing Compound I-72 with acaricides can improve the control efficacy, reduce the dosage, and broaden the insecticidal spectrum. For example, when Compound I-72 and pyridaben are mixed in a ratio range of 1:10 to 10:1, the activity against spider mites can be greatly improved; therefore, the synergistic compound composition of the present invention can be used for preparing insecticidal and acaricidal drugs.

[0019] The synergistic compound composition of the present invention can be used to control pests, mite pests, and underground pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees, etc.; especially for controlling mite pests on crops, particularly in the application of controlling mite pests on crops such as citrus, cotton, apples, flowers or vegetables.

[0020] In particular, the synergistic compound composition of the present invention can be especially used for controlling pests of the order Acarina. A non-exhaustive list of these pests includes, but is not limited to: for example, Panonychus citri, Tetranychus cinnabarinus, Panonychus ulmi, Tetranychus urticae, Tetranychus viennensis, Oligonychus ununguis, Eotetranychus kankitus, Brevipalpus phoenicis, Bryobia praetiosa, Aceria tulipae, Colomerus vitis, Calacarus carinatus, Phyllocoptes oleivora ashmead, Polyphagotarsonemus latus, Rhizoglyphus rostochiensis, Acarus siro, Aceria sheldoni, Aculus schlechtendali, Acalitus spp., Aculus spp, Acaricalus spp., Aceria spp., Amblyomma spp., Argas spp., Boophilus spp, Brevipalpus spp., Bryobia spp, Calipitrimerus spp., Chorioptes spp., Dermanyssus gallinae, Dermatophagoides spp, Eotetranychus spp, Eriophyesspp., Hemitarsonemus spp, Hyalomma spp., Ixodes spp., Olygonychus spp, Ornithodoros spp) Polyphagotarsonemus latus, Panonychus spp., Phyllocoptruta oleivora, Phytonemus spp., Polyphagotarsonemus spp, Psoroptes spp., Rhipicephalus spp., Rhizoglyphus spp., Sarcoptes spp., Steneotarsonemus spp, Tarsonemus spp., Tetranychus spp., etc.;.

[0021] In particular, the synergistic compound composition of the present invention can be especially used for controlling Panonychus citri, Phyllocoptruta oleivora, Eotetranychus kankitus, Tetranychus viennensis, Panonychus ulmi, Tetranychus urticae, Polyphagotarsonemus latus, Calacarus carinatus, Brevipalpus obovatus, Calepitrimerus theae, Tetranychus cinnabarinus, Tetranychus truncatus, Tetranychus turkestani, Tetranychus dunhuangensis, Penthaleus major, Oligonychus tritici, Aceria jujubifolia, Phyllocnistis citrella, citrus scale insects, Lyonetia clerkella, Grapholita molesta, Leguminivora glycinivorella, Lithocolletis ringoniella, Ectropis obliqua, Euproctis pseudoconspersa, Empoasca vitis, Psylla chinensis, Nilaparvata lugens, Trialeurodes vaporariorum, Liriomyza sativae, Phyllotreta striolata, Chilo suppressalis, Tryporyza incertulas, Ostrinia furnacalis, Etiella zinckenella, Diaphania indica, Lissorhoptrus oryzophilus, Helicoverpa armigera, Pectinophora gossypiella, Myzus persicae, Aphis glycines, Lipaphis erysimi, Sitobion avenae, Aphis gossypii, Plutella xylostella, Mythimna separata, Pieris rapae, Helicoverpa assulta, Cerambycidae and other pests and mites.

[0022] The synergistic compound composition of the present invention can be applied at an effective dose to the pests / mites to be controlled and / or their habitats. Therefore, the synergistic compound composition of the present invention provides a method for controlling or preventing plant pests.

[0023] The active ingredients in the synergistic compound composition of the present invention can be applied to pests, plants and their habitats simultaneously (for example, as a pre-formulated mixture or used in a tank mix) or in a suitable order.

[0024] Advantages of the present invention: The binary composition obtained by compounding compound I-72 with an acaricide in an appropriate ratio exhibits a strong synergistic effect, thereby improving control efficacy, reducing dosage, and expanding the insecticide spectrum. Specifically, 1. The two active components A and B in the compound have different mechanisms of action, which can delay the development of insecticide resistance in pests and mites. 2. The insecticide spectrum is expanded and can be used to control a variety of pests and mites on crops such as fruit trees, vegetables, ornamental plants, tea, and cotton. 3. The pesticide dosage is reduced, reducing pesticide residues on crops and enhancing environmental friendliness. 4. The two active components A and B are compounded within an appropriate ratio to enhance the control efficacy of pests and mites. DETAILED DESCRIPTION

[0025] The following examples are provided to illustrate the present invention in detail and facilitate a further understanding of the present invention. Active component A is selected from compound I-72 (prepared as described in patent document CN112457288, with a content of 97%); active component B and other raw materials are commercially available products, and each component is measured by weight percentage.

[0026] Example 1: Indoor joint toxicity test of compound I-72 and pyridabenzone against Tetranychus cinnabarinus

[0027] Test target: Adult mites of Tetranychus cinnabarinus (boisduval), a susceptible strain reared indoors.

[0028] Preparation of drug solution: According to different test requirements, the test samples were accurately weighed using an electronic analytical balance. The original drug was dissolved in acetone and then diluted with 0.1% Tween 80 water to form a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0029] Test Method: The potted seedling spray method was used to determine the activity against adult Tetranychus cinnabarinus mites. First, adult Tetranychus cinnabarinus mites of uniform size were inoculated onto the leaves of kidney bean seedlings at the stage of expansion of the first pair of true leaves. The number of adult mites was counted after stabilization. The spray was then applied evenly, starting from the lowest to the highest dose, using 1.5 mL per plant. Three replicates were applied per treatment, with a blank control.

[0030] The treated specimens were placed in an observation room where the temperature, humidity, and light levels could be adjusted as needed. After 72 hours, the number of dead and live mites was counted. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using DPS data processing software. The toxicity regression equations and LC values for each test single agent and each combination of different ratios were calculated. 50 The co-toxicity coefficient of each ratio was calculated using the Sun Yunpei method to evaluate the mixing effect. The test results are shown in Table 1.

[0031] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0032]

[0033] Where: ATI - the measured toxicity index of the mixture; S - the LC of the standard agent 50 ; M - the LC of the mixture 50 .

[0034] TTI = TIA × PA + TIB × PB

[0035] Where: TTI - the theoretical toxicity index of the mixture; TIA - the toxicity index of agent A; PA - the percentage content of agent A in the mixture; TIB - the toxicity index of agent B; PB - the percentage content of agent B in the mixture.

[0036]

[0037] Where: CTC - the co-toxicity coefficient; ATI - the measured toxicity index of the mixture; TTI - the theoretical toxicity index of the mixture.

[0038] The co-toxicity coefficient (CTC) of the compound mixture: CTC ≥ 120 indicates a synergistic effect; 80 < CTC < 120 indicates an additive effect; CTC ≤ 80 indicates an antagonistic effect.

[0039] Table 1 Results of indoor joint toxicity determination of compound I72 and pyridaben against Tetranychus cinnabarinus

[0040]

[0041] Example 2: Indoor joint toxicity determination test of compound I-72 and cyazofamid against Tetranychus urticae Koch adults, a sensitive strain reared indoors.

[0042] Preparation of the liquid medicine: According to different test requirements, accurately weigh the test samples respectively with an electronic analytical balance. The technical materials are dissolved in acetone and then diluted with 0.1% Tween 80 water into a series of liquid medicines with a certain concentration gradient according to the test design dose.

[0043] Test method: The pot seedling spraying method was used to determine the activity against Tetranychus urticae Koch adults. First, Tetranychus urticae Koch adults of the same size were placed on the leaves of kidney bean seedlings at the stage of the first pair of true leaves unfolding, and the base number was counted after the adults were stable; then, spraying was carried out evenly in the order from low dose to high dose according to the test design, 1.5 mL per plant, with 3 replicates for each treatment, and a blank control was set up separately.

[0044] It should be noted that there seems to be an error in the expression "120<CTC<120" in the original text, which is corrected to "80 < CTC < 120" in the translation.Place the processed test materials in the observation chamber, and the temperature, humidity, and light in the observation chamber can be adjusted as required. After 72 hours, investigate the number of dead and live mites. Calculate the corrected mortality rate using the Abbott formula, and perform statistical analysis using DPS data processing software to obtain the toxicity regression equations, LC 50 values and 95% confidence limits for each tested single agent and each different mixed ratio. Then, use the Sun y-p method to calculate the co-toxicity coefficient of each ratio to evaluate the mixing effect (the same as in Example 1). The test results are shown in Table 2.

[0045] Table 2 Results of indoor combined toxicity determination of the mixture of Compound I72 and cyazofamid against Tetranychus urticae

[0046]

[0047] The description of the above embodiments is only used to help understand the method and its core idea of the present invention. It should be noted that for those of ordinary skill in the art of this technology, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention. From the above technical solutions, it can be seen that the insecticidal and acaricidal composition described in the present invention has the advantages of improving the drug efficacy, expanding the insecticidal spectrum, reducing the dosage of pesticides, reducing the residue of pesticides on crops, reducing environmental pollution, being safe for humans and animals, having good environmental compatibility, and being difficult for pests to develop resistance. The composition of the present invention can be applied to the control of various pests and mites on a variety of crops and can be widely applied in the agricultural field.

Claims

1. A synergistic compound composition of two active components, characterized in that: The composition comprises two active components, A and B. Component A is selected from piperic acid derivative compounds with the following structures, and component B is selected from pyridaben; the weight ratio of the two active components A and B is 1:50 to 50:1; wherein, the structural formula of the piperic acid derivative compound is as follows:

2. The composition according to claim 1, characterized in that: The weight ratio of the two active components A and B is 1:20 to 20:

1.

3. The composition according to claim 2, characterized in that: The weight ratio of the two active components A and B is 1:10 to 10:

1.

4. Use of the composition according to claim 1, characterized in that: The composition is used for preparing insecticidal and acaricidal drugs for controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, and evergreen trees.

5. The composition according to claim 1, characterized in that: The use of the composition for preventing or controlling pests, their larvae and eggs of Acarina, Lepidoptera, Hemiptera, Homoptera, Coleoptera, Diptera, and Thysanoptera.

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