Reagent containing amino-oligosaccharin and indoleacetic acid as well as preparation method and application of reagent

Abstract

The invention discloses a reagent containing amino-oligosaccharin and indoleacetic acid and a preparation method and application thereof.According to the reagent, amino-oligosaccharin and indoleacetic acid are compounded according to a specific proportion, the two components form an efficient synergistic system, ginger root development is remarkably promoted, stress resistance is improved, and the effect of preventing and treating ginger root knot nematode diseases is achieved; by adding a functional auxiliary agent, sodium n-alkyl benzene sulfonate, rhamnolipid and N-hydroxysuccinimide are used as components of the functional auxiliary agent, so that the functional auxiliary agent can be chelated with indoleacetic acid to fix and delay degradation of the indoleacetic acid in soil, and the surface tension of a solution interface can be reduced; the dispersion and stability of the effective components in the reagent in the system are promoted, and a proper amount of penetration enhancer and stabilizer are also added, so that the photolysis and oxidation of the indoleacetic acid are reduced, the activity in the storage and use process is improved, and the application of the active components is more facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of plant growth additives, and in particular, to a reagent containing oligosaccharins and indoleacetic acid, a preparation method thereof, and an application thereof. Background Art

[0002] Ginger is a shallow-rooted crop. Its germination is slow in the early stage and it is sensitive to temperature. It takes 25 - 30 days from sowing to emergence, and 25 - 30 days from emergence to having 5 - 6 leaves. During this period, the land lies idle for up to 50 - 60 days. According to the conventional operation, ginger seedlings with a height of 15 - 20 cm are lifted out of the seedling raising bed, transplanted, and placed in a greenhouse or a large arch shed for 5 - 10 days of slow seedling growth, and then transplanted into a field planting base together with the nutrient pots. It can be known that the fibrous roots of ginger are adventitious roots generated from the base of the young buds. The number is small but they are long, mainly responsible for absorbing water and nutrients. In the current planting technology, when separating the ginger seedlings from the mother ginger, it is easy to damage the fibrous roots and the separation wound is large, increasing the probability of infecting ginger blast.

[0003] Moreover, ginger is generally propagated asexually during the planting process and has not gone through a sexual generation. It is easy to infect and accumulate various plant viruses, such as tobacco mosaic virus and cucumber mosaic virus, etc. Its roots are weak and are easily affected by soil-borne diseases, resulting in the gradual degradation of many excellent varietal characters, leading to a decrease in yield and quality, especially a reduction in stress resistance and the increasing prevalence of ginger blast, and the problem of poor drug control effect. Therefore, during the growth cycle of ginger, improving the growth quality of its roots helps to reduce the incidence rate.

[0004] In the prior art, there are also additives for ginger planting. For example, the patent with the Chinese patent application number CN202510198587.X discloses a root-promoting agent suitable for rooting of cut ginger, a ginger cuttage seedling raising method and an application thereof. Through the action of 4 components, namely, naphthylacetic acid - sodium nitrophenolate diluted 200 times, microbial agent polyglutamic acid diluted 500 times, plant rooting powder diluted 500 times, and mepiquat chloride diluted 10,000 times, the comprehensive indexes of the above-ground part, plant growth amount, and seedling roots of ginger cuttage seedling raising reach the optimal. Another example is the patent with the Chinese patent application number CN201710629462.3, which discloses a cold-resistant agent for Zingiberaceae, a preparation method thereof and a using method thereof. By compounding components such as salicylic acid, paclobutrazol, betaine, L-proline, and gibberellin, it is used as a cold-resistant agent for Zingiberaceae to improve the stress resistance of ginger. However, the existing additives for ginger planting in the prior art still have the problem that they cannot simultaneously meet the growth promotion effect and improve its stress resistance, and thus it is difficult to ensure the yield and quality. Summary of the Invention

[0005] Based on this, in order to solve one of the above problems, the present invention provides a reagent containing oligosaccharins and indoleacetic acid, a preparation method thereof, and an application thereof. The specific technical solutions are as follows:

[0006] A reagent containing chitosan oligosaccharide and indole acetic acid, the reagent comprising the following components in parts by weight:

[0007] 7 to 9 parts of chitosan oligosaccharide, 1 to 3 parts of indole acetic acid, 0.3 to 0.8 parts of functional adjuvant, 100 parts of solvent, 0.1 to 0.3 parts of penetration enhancer, 0.01 to 0.03 parts of dispersant, and 0.1 to 0.5 parts of stabilizer.

[0008] Preferably, the preparation method of the functional adjuvant is:

[0009] Dissolve linear alkylbenzene sulfonate and rhamnolipid in deionized water, then add N-hydroxysuccinimide, stir magnetically, adjust the pH to 5.0 - 6.0 with citric acid, and then stir magnetically at 30°C - 45°C for 3h - 5h to obtain the functional adjuvant.

[0010] Preferably, the weight ratio of linear alkylbenzene sulfonate, rhamnolipid, deionized water, and N-hydroxysuccinimide is: (1 - 3) : (1 - 2) : (8 - 12) : (1 - 2).

[0011] Preferably, the solvent is an ethanol aqueous solution, and the mass percentage concentration of ethanol in the ethanol aqueous solution is 10% - 50%.

[0012] Preferably, the penetration enhancer is at least one of polyglutamic acid and sodium humate.

[0013] Preferably, the dispersant is at least one of sodium polyacrylate and sodium lignosulfonate.

[0014] Preferably, the stabilizer is trehalose.

[0015] In addition, the present invention also provides a preparation method of a reagent containing chitosan oligosaccharide and indole acetic acid, the preparation method comprising the following steps:

[0016] Add the stabilizer to the solvent, heat to 40°C - 50°C, and then, under light-shielded conditions, sequentially add chitosan oligosaccharide, indole acetic acid, and the functional adjuvant, stir well, and then add the penetration enhancer and the dispersant, and mix evenly to obtain the reagent containing chitosan oligosaccharide and indole acetic acid.

[0017] The present invention also provides an application of a reagent containing chitosan oligosaccharide and indole acetic acid, and the application is the application of the reagent containing chitosan oligosaccharide and indole acetic acid in the cultivation of Zingiberaceae plants.

[0018] Preferably, when the reagent containing amino-oligosaccharin and indoleacetic acid is used in the cultivation of Zingiberaceae plants, it needs to be diluted with water so that the mass percentage content of amino-oligosaccharin is 0.9%, the mass percentage content of indoleacetic acid is 0.1%, and the root irrigation method is 1000 mL / mu to 2000 mL / mu.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. In the present invention, amino-oligosaccharin and indoleacetic acid are compounded in a specific ratio. Amino-oligosaccharin can activate the defense signal pathway of ginger roots, enhance the extensibility of cell walls, and indoleacetic acid can precisely stimulate the differentiation of root primordia. The two components form an efficient synergistic system, prolong the activity of the root meristem zone, act directly on the ginger root zone through drip irrigation or sprinkler irrigation systems, achieve rapid root meristem, increase lateral roots, and enlarge ginger tubers, significantly promote the development of ginger roots, improve stress resistance, and have the effect of preventing and controlling ginger root-knot nematode diseases, which helps to increase yield and quality.

[0021] 2. In the present invention, by adding functional adjuvants and using linear alkylbenzene sulfonate, rhamnolipid, and N-hydroxysuccinimide as components of the functional adjuvants, not only can they chelate and fix indoleacetic acid to delay its degradation in the soil, but also can reduce the surface tension of the solution interface, promote the dispersion and stability of the active ingredients in the reagent system.

[0022] 3. An appropriate amount of penetration enhancer and stabilizer are also added to the reagent system formed in the present invention, which helps to reduce the photolysis and oxidation of indoleacetic acid, improve its activity during storage and use, is more conducive to the application of active ingredients, and solves the problem that the additives used in ginger cultivation in the prior art cannot simultaneously meet the growth promotion effect and improve its stress resistance.

[0023] 4. The components of the reagent of the present invention are safe, environmentally friendly, have significant effects, and the preparation process and application method are simple and highly operable. Specific Embodiments

[0024] The following is a detailed description in conjunction with specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. The raw materials and reagents used in the examples are all commercially available unless otherwise specified.

[0025] Example 1:

[0026] A preparation method of a reagent containing amino-oligosaccharin and indoleacetic acid, comprising the following steps:

[0027] By weight parts, dissolve 2 parts of linear alkylbenzene sulfonate and 1 part of rhamnolipid in 10 parts of deionized water, then add 1 part of N-hydroxysuccinimide, stir magnetically, adjust the pH to 5.0 with citric acid, and stir magnetically at 40 °C for 3 h to obtain a functional auxiliary agent;

[0028] By weight parts, add 0.2 parts of trehalose to 100 parts of a solvent (an ethanol aqueous solution with a mass percentage concentration of 20%), heat to 40 °C, and then, under light-shielded conditions, successively add 9 parts of oligosaccharins, 1 part of indoleacetic acid, and 0.5 part of the functional auxiliary agent. After stirring well, add 0.1 part of polyglutamic acid and 0.02 part of sodium lignosulfonate, and mix evenly to obtain a reagent containing oligosaccharins and indoleacetic acid.

[0029] Example 2:

[0030] A preparation method of a reagent containing oligosaccharins and indoleacetic acid, comprising the following steps:

[0031] By weight parts, dissolve 1 part of linear alkylbenzene sulfonate and 2 parts of rhamnolipid in 11 parts of deionized water, then add 1 part of N-hydroxysuccinimide, stir magnetically, adjust the pH to 5.3 with citric acid, and stir magnetically at 35 °C for 4 h to obtain a functional auxiliary agent;

[0032] By weight parts, add 0.3 parts of trehalose to 100 parts of a solvent (an ethanol aqueous solution with a mass percentage concentration of 20%), heat to 40 °C, and then, under light-shielded conditions, successively add 9 parts of oligosaccharins, 1 part of indoleacetic acid, and 0.6 part of the functional auxiliary agent. After stirring well, add 0.2 part of polyglutamic acid and 0.03 part of sodium lignosulfonate, and mix evenly to obtain a reagent containing oligosaccharins and indoleacetic acid.

[0033] Example 3:

[0034] A preparation method of a reagent containing oligosaccharins and indoleacetic acid, comprising the following steps:

[0035] By weight parts, dissolve 1 part of linear alkylbenzene sulfonate and 1 part of rhamnolipid in 10 parts of deionized water, then add 2 parts of N-hydroxysuccinimide, stir magnetically, adjust the pH to 5.5 with citric acid, and stir magnetically at 30 °C for 5 h to obtain a functional auxiliary agent;

[0036] By weight, 0.4 parts of trehalose are added to 100 parts of a solvent (an aqueous ethanol solution with a mass percentage concentration of 20%), heated to 45 °C, and then, under light-shielded conditions, 9 parts of oligosaccharins, 1 part of indole-3-acetic acid, and 0.7 part of a functional adjuvant are added in sequence. After stirring well, 0.3 part of polyglutamic acid and 0.03 part of sodium lignosulfonate are added and mixed evenly to obtain a reagent containing oligosaccharins and indole-3-acetic acid.

[0037] Example 4:

[0038] A method for preparing a reagent containing oligosaccharins and indole-3-acetic acid, comprising the following steps:

[0039] By weight, 3 parts of linear alkylbenzene sulfonate and 1 part of rhamnolipid are dissolved in 12 parts of deionized water, then 2 parts of N-hydroxysuccinimide are added, and magnetic stirring is carried out. The pH is adjusted to 6.0 with citric acid, and magnetic stirring is carried out at 45 °C for 5 h to obtain a functional adjuvant;

[0040] By weight, 0.5 parts of trehalose are added to 100 parts of a solvent (an aqueous ethanol solution with a mass percentage concentration of 20%), heated to 50 °C, and then, under light-shielded conditions, 9 parts of oligosaccharins, 1 part of indole-3-acetic acid, and 0.8 part of a functional adjuvant are added in sequence. After stirring well, 0.2 part of sodium humate and 0.02 part of sodium lignosulfonate are added and mixed evenly to obtain a reagent containing oligosaccharins and indole-3-acetic acid.

[0041] Example 5:

[0042] A method for preparing a reagent containing oligosaccharins and indole-3-acetic acid, comprising the following steps:

[0043] By weight, 2 parts of linear alkylbenzene sulfonate and 2 parts of rhamnolipid are dissolved in 12 parts of deionized water, then 2 parts of N-hydroxysuccinimide are added, and magnetic stirring is carried out. The pH is adjusted to 5.6 with citric acid, and magnetic stirring is carried out at 40 °C for 4 h to obtain a functional adjuvant;

[0044] By weight, 0.4 parts of trehalose are added to 100 parts of a solvent (an aqueous ethanol solution with a mass percentage concentration of 20%), heated to 45 °C, and then, under light-shielded conditions, 9 parts of oligosaccharins, 1 part of indole-3-acetic acid, and 0.7 part of a functional adjuvant are added in sequence. After stirring well, 0.3 part of sodium humate and 0.02 part of sodium lignosulfonate are added and mixed evenly to obtain a reagent containing oligosaccharins and indole-3-acetic acid.

[0045] Comparative Example 1:

[0046] Comparative Example 1 is different from Example 5 in that 9 parts by weight of chitosan oligosaccharide and 1 part by weight of indole acetic acid are added to 100 parts of a solvent (an aqueous ethanol solution with a mass percentage concentration of 20%) to obtain a reagent.

[0047] Comparative Example 2:

[0048] Comparative Example 2 is different from Example 5 in that linear alkylbenzene sulfonate is not added, and the others are the same as Example 5.

[0049] Comparative Example 3:

[0050] Comparative Example 3 is different from Example 5 in that rhamnolipid is not added, and the others are the same as Example 5.

[0051] Comparative Example 4:

[0052] Comparative Example 4 is different from Example 5 in that N-hydroxysuccinimide is not added, and the others are the same as Example 5.

[0053] Comparative Example 5:

[0054] Comparative Example 5 is different from Example � in that the functional adjuvant is not added, and the others are the same as Example 5.

[0055] Comparative Example 6:

[0056] Comparative Example 6 is different from Example 5 in that trehalose (stabilizer) is not added, and the others are the same as Example 5.

[0057] Comparative Example 7:

[0058] Comparative Example 7 is different from Example 5 in that chitosan oligosaccharide is not added, and the others are the same as Example 5.

[0059] Comparative Example 8:

[0060] Comparative Example 8 is different from Example 5 in that indole acetic acid is not added, and the others are the same as Example 5.

[0061] Comparative Example 9:

[0062] Comparative Example 9 is different from Example 5 in that sodium humate (penetration enhancer) is not added, and the others are the same as Example 5.

[0063] Comparative Example 10:

[0064] Comparative Example 10 is a blank control group, that is, the same amount of clear water is irrigated.

[0065] 1. The reagent samples containing amino-oligosaccharins and indoleacetic acid prepared in Examples 1-5 and the reagent comparison samples prepared in Comparative Examples 1-9 were subjected to stability tests.

[0066] Among them, the stability test was as follows: placed in a brown glass bottle, left standing at room temperature for 3 months, then treated at a rotation speed of 100 r / min for 5 min, and then left standing for 1 h. The relative environmental humidity was 65%. Three parallels were set for each group. The residual amounts of the active ingredients (amino-oligosaccharins, indoleacetic acid) were detected by HPLC, and the degradation percentage was calculated: decomposition rate (%) = (1 - Ct / C0) × 100% (Ct: concentration at time t, C0: initial concentration). The average value was taken, and the changes in the reagent were observed with the naked eye. The results are shown in Table 1 below.

[0067] Table 1: Stability

[0068] Group Appearance change Decomposition rate % Example 1 No obvious change, excellent stability ≤1 Example 2 No obvious change, excellent stability ≤1 Example 3 No obvious change, excellent stability ≤1 Example 4 No obvious change, excellent stability ≤1 Example 5 No obvious change, excellent stability ≤1 Control Example 1 Slight flocculation, poor stability ≥20 Control Example 2 Slight flocculation, average stability ≥18 Control Example 3 Slight flocculation, average stability ≥16 Control Example 4 Slight flocculation, average stability ≥20 Control Example 5 Turbid, poor stability ≥25 Control Example 6 Slight flocculation, average stability ≥8 Control Example 7 No obvious change, excellent stability ≤1 Control Example 8 No obvious change, excellent stability ≤1 Control Example 9 No obvious change, excellent stability ≤1

[0069] It can be seen from the data analysis in Table 1 that the reagent obtained by compounding in this application has more excellent stability. In Comparative Example 1, only amino-oligosaccharins and indoleacetic acid were mixed and added to an ethanol aqueous solution with a mass percentage concentration of 20%, which could be used immediately after preparation and could not be stably stored for a long time. Moreover, after use, it was also easily affected by the environment, thereby affecting the stability. In Comparative Examples 2-4, the preparation components of the functional adjuvant were different. In Comparative Example 5, no functional adjuvant was added, but different degrees of flocculation and decomposition of the active ingredients occurred in Comparative Examples 2-5, indicating that the functional adjuvant in this application can play a role in improving the stability of the reagent, which is more beneficial to the storage stability and use stability of the reagent. In Comparative Example 6, no stabilizer was added, but it also made the stability of the reagent worse, and the decomposition rate of the active ingredient was higher than that in Example 5, indicating that adding an appropriate amount of stabilizer helps to provide the storage stability of the reagent. It shows that after the component optimization in this application, it has more excellent application stability as a whole. The components interact with each other and produce a chelating effect, and the activity is more stable.

[0070] 2. The reagent samples containing amino-oligosaccharins and indoleacetic acid prepared in Examples 1-5 and the reagent comparison samples prepared in Comparative Examples 1-9 were respectively subjected to small-scale laboratory pot experiments and compared with a blank control group.

[0071] The method is as follows: Each group is set with 10 repetitions. For each group of experiments, select robust and uniform-sized tissue culture seedlings of ginger, and rinse and disinfect them with carbendazim. Transplant the disinfected ginger seedlings into flower pots that have been washed and dried (the diameter of the flower pot mouth is 20 cm and the depth is 15 cm). Transplant two ginger seedlings into each pot. When the ginger seedlings grow to the 10th day, inoculate the corresponding samples or control samples (both the samples and the control samples need to be diluted 10 times with water before use). Observe regularly at room temperature, and water with clear water regularly. Record the growth conditions of ginger at different times. The results are shown in Table 2 below.

[0072] Table 2: Growth conditions of ginger

[0073]

[0074]

[0075] From the data analysis in Table 2, it can be seen that after optimizing the composition and component ratio of the reagent in this application, it has a more excellent growth promotion effect. And after the components in this application are compounded, the active ingredients can be better absorbed and utilized by ginger to achieve the purpose of promoting growth. The ginger in the treatment groups of Examples 1-5 grows uniformly, has well-developed roots, and there is no problem of ginger root-knot nematode disease. Among them, the treatment group of Example 5 has the best effect.

[0076] Third, conduct a field experiment on the reagent prepared in the above-mentioned preferred Example 5. When the reagent containing oligosaccharins and indoleacetic acid prepared in Example 5 is used in the cultivation of ginger, add water for dilution so that the mass percentage content of oligosaccharins is 0.9%, and the mass percentage content of indoleacetic acid is 0.1%. And irrigate the roots at a rate of 1000 mL / mu, irrigate once every 7 days, and irrigate continuously for 3 times. Use the blank control as the control group. After 30 days of treatment, observe the growth conditions of ginger in the field. The results are shown in Table 3 below.

[0077] Table 3: Results of field experiment

[0078] Group Plant height cm Root length / cm Fresh weight / g / plant Number of buds / piece Leaf SPAD value Blank control group 28.4 8.9 21.3 3 31.2 Example 5 38.2 16.2 49.7 5 49.6

[0079] From the analysis of the field experiment, it can be seen that in this application, oligosaccharins and indoleacetic acid are compounded in a specific ratio. Oligosaccharins can activate the defense signal pathway of ginger roots, enhance the extensibility of cell walls, and indoleacetic acid can precisely stimulate the differentiation of root primordia. The two components form an efficient synergistic system, extend the activity of the root meristematic zone, and directly act on the ginger root zone through drip irrigation or sprinkler irrigation systems, realizing rapid root meristem, increased lateral roots, and ginger tuber swelling, significantly promoting the development of ginger roots, improving stress resistance, and significantly increasing the yield and quality.

[0080] The foregoing description of the specific exemplary embodiments of the present invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many modifications and variations are possible in light of the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical applications, so that those skilled in the art can implement and utilize various different exemplary embodiments of the present invention, as well as various different selections and modifications. The scope of the present invention is intended to be defined by the claims and their equivalents.

Claims

1. A reagent containing amino-oligosaccharin and indoleacetic acid, characterized in that, The reagent comprises the following components in parts by weight: 7 to 9 parts of amino-oligosaccharins, 1 to 3 parts of indoleacetic acid, 0.3 to 0.8 part of functional auxiliary agent, 100 parts of solvent, 0.1 to 0.3 part of penetration enhancer, 0.01 to 0.03 part of dispersant and 0.1 to 0.5 part of stabilizer.

2. The reagent according to claim 1, wherein The preparation method of the functional auxiliary agent is as follows: Dissolve linear alkylbenzene sulfonate and rhamnolipid in deionized water, then add N-hydroxysuccinimide, stir magnetically, adjust the pH to 5.0 - 6.0 with citric acid, and then stir magnetically at 30°C - 45°C for 3h - 5h to obtain the functional auxiliary agent.

3. The reagent according to claim 2, wherein The weight ratio of linear alkylbenzene sulfonate, rhamnolipid, deionized water and N-hydroxysuccinimide is: (1 - 3):(1 - 2):(8 - 12):(1 - 2).

4. The reagent according to claim 1, wherein The solvent is an ethanol aqueous solution, and the mass percentage concentration of ethanol in the ethanol aqueous solution is 10% - 50%.

5. The reagent according to claim 1, characterized in that, The penetration enhancer is at least one of polyglutamic acid and sodium humate.

6. The reagent according to claim 1, wherein The dispersant is at least one of sodium polyacrylate and sodium lignosulfonate.

7. The reagent according to claim 1, wherein The stabilizer is trehalose.

8. A preparation method of a reagent containing amino-oligosaccharins and indoleacetic acid, characterized in that, The preparation method is used to prepare the reagent containing amino-oligosaccharins and indoleacetic acid as described in any one of claims 1 - 7. The preparation method comprises the following steps: Add the stabilizer to the solvent, heat to 40°C - 50°C, and then, under the condition of avoiding light, sequentially add amino-oligosaccharins, indoleacetic acid and the functional auxiliary agent, stir well, and then add the penetration enhancer and the dispersant and mix evenly to obtain the reagent containing amino-oligosaccharins and indoleacetic acid.

9. Application of a reagent containing amino-oligosaccharins and indoleacetic acid, characterized in that, The application is the application of the reagent containing amino-oligosaccharins and indoleacetic acid as described in any one of claims 1 - 7 in the planting of Zingiberaceae plants.

10. The application according to claim 9, wherein When the reagent containing amino-oligosaccharins and indoleacetic acid is used in the planting of Zingiberaceae plants, it needs to be diluted with water so that the mass percentage content of amino-oligosaccharins is 0.9% and the mass percentage content of indoleacetic acid is 0.1%, and it is applied by root irrigation at a rate of 1000 mL / mu - 2000 mL / mu.

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