Sophora alopecuroides extract composite biological medical fertilizer, preparation method and application

Abstract

The invention discloses a sophora alopecuroide extract composite biological medical fertilizer, and a preparation method and application thereof. The composite biological medical fertilizer comprises a sophora alopecuroide total alkaloid preparation and superoxide dismutase powder, the sophora alopecuroide total alkaloid preparation and the superoxide dismutase powder are compounded according to the mass ratio of the effective components of (70-72): (30-28). The content of total alkaloids in the sophora alopecuroides in the total biological preparation is 5-6%. The content of superoxide dismutase in the superoxide dismutase powder is 9000 to 11000 u / g. The invention has the advantages that the plant resistance can be improved, the plant growth is promoted, and the fruit quality is improved.

Description

Technical Field

[0001] The present invention belongs to the field of biological medicated fertilizers, and particularly relates to a Sophora alopecuroides extract composite biological medicated fertilizer, a preparation method and an application thereof. Background Art

[0002] In recent years, the unreasonable use of chemical fertilizers and pesticides has led to problems such as the reduction of the quality of agricultural products, the enhancement of the drug resistance of diseases, the aggravation of environmental pollution, and the harm to the health of humans and livestock, which have attracted wide attention. Biological medicated fertilizers are a new type of medicated fertilizers that have emerged in response to various problems existing in chemical medicated fertilizers. Because of their relatively weak toxicity, strong pertinence, not easy to produce drug resistance, and high safety. Plant-derived and biocontrol microbial medicated fertilizers have become the current development trend. This type of new medicated fertilizer has the characteristics of being environmentally friendly and green, which conforms to the development direction of the country's ecological civilization construction, and also meets the overall requirements of "reducing chemical fertilizers, reducing pesticides, and controlling diseases" in agricultural production and green prevention and control.

[0003] Superoxide Dismutase (SOD) has stable properties and can be applied to most production scenarios. It has the function of catalyzing the disproportionation reaction of superoxide, thereby protecting cells from the damage of oxygen exposure. It is an important antioxidant enzyme. A large number of studies have shown that superoxide dismutase fertilizers have the characteristics of strengthening plants, improving fruit quality, increasing crop yields, and enhancing the stress resistance of crops, and have good practical application effects.

[0004] At present, the development of new plant-derived medicated fertilizers that are green, environmentally friendly and highly functional is of great significance for agricultural production practice. The research and development of new plant-derived medicated fertilizers helps to achieve the environmentally friendly, green, efficient and intensive development of agriculture. At the same time, it also helps crop growers to increase production and income, promote the efficient use of resources, and shows a broad market prospect. Summary of the Invention

[0005] The purpose of the present invention is to provide a Sophora alopecuroides extract composite biological medicated fertilizer, a preparation method and an application thereof, which have the functions of preventing diseases and promoting growth, and improving the quality of crops.

[0006] To achieve the above purpose, the technical solutions adopted by the present invention include:

[0007] A Sophora alopecuroides extract composite biological medicated fertilizer, which comprises a total alkaloid preparation of Sophora alopecuroides and a superoxide dismutase powder;

[0008] The total alkaloid preparation of Sophora alopecuroides and the superoxide dismutase powder are compounded according to the mass ratio of active ingredients of 70-72:30-28.

[0009] Optionally, the content of total alkaloids in the total biological preparation of Sophora alopecuroides is 5%-6%.

[0010] Optionally, the content of superoxide dismutase in the superoxide dismutase powder is 9000 - 11000 u / g.

[0011] Optionally, the total alkaloids of Sophora alopecuroides are extracted from Sophora alopecuroides by the cyclic percolation method;

[0012] The extraction by the cyclic percolation method includes: the extraction solvent is ethanol with a mass percentage concentration of 66.7%, the flow rate of the extraction device is 3.69 m / s, the extraction time is 68 min, and the extraction temperature is 49.6 °C.

[0013] Optionally, the preparation of the total alkaloids of Sophora alopecuroides is a wettable powder of the total alkaloids of Sophora alopecuroides;

[0014] The wettable powder of the total alkaloids of Sophora alopecuroides includes 5% - 10% of the total alkaloid extract of Sophora alopecuroides, 10% of sodium dodecylbenzenesulfonate, 15% of dispersant NNO, and 65% - 70% of bentonite.

[0015] The preparation method of the Sophora alopecuroides extract composite biological medicine fertilizer according to any one of the present invention includes:

[0016] ① Extract the total alkaloids of Sophora alopecuroides by the cyclic percolation method;

[0017] ② Prepare the preparation of the total alkaloids of Sophora alopecuroides: according to the formula amount, use the total alkaloids of Sophora alopecuroides obtained in step ① to prepare a wettable powder;

[0018] ③ Compound the superoxide dismutase powder and the wettable powder of the total alkaloids of Sophora alopecuroides according to the mass ratio of the active ingredients to prepare a composite biological medicine fertilizer.

[0019] Optionally, the extraction by the cyclic percolation method includes: the extraction solvent is ethanol with a mass percentage concentration of 66.7%, the flow rate of the extraction device is 3.69 m / s, the extraction time is 68 min, and the extraction temperature is 49.6 °C.

[0020] The application of the Sophora alopecuroides extract composite biological medicine fertilizer according to any one of the present invention for preparing a crop fertilizer.

[0021] Optionally, the crops include peppers, cucumbers and / or tomatoes, and the crop fertilizer is used for promoting the growth of crops, increasing production, improving quality and / or increasing stress resistance.

[0022] Optionally, the crop fertilizer is used for preventing and treating tomato gray mold.

[0023] The advantages of the present invention are:

[0024] ① At present, there are many reports on biological pharmaceutical fertilizers, but the research on plant-derived biological pharmaceutical fertilizers accounts for a relatively small proportion. Therefore, it is of great significance to develop new plant-derived pharmaceutical fertilizers that are green, environmentally friendly and highly functional.

[0025] ② Using sophora alopecuroides alkaloids and superoxide dismutase powder as raw materials to compound and process into a new biological pharmaceutical fertilizer, its application shows good growth-promoting and resistance-inducing activities on crops, and can better serve agricultural production.

[0026] ③ There are still many problems in the extraction process of alkaloids. Conventional traditional methods for extracting alkaloids require a large amount of solvents, long extraction time and low efficiency, while new extraction technologies often require high equipment investment and management costs, which do not conform to the concept of sustainable development. The use of the cyclic percolation method has a good promoting effect on the full utilization of sophora alopecuroides resources. Specific implementation manners

[0027] The substantial features of the present invention can be embodied in the following embodiments, but these embodiments are only for illustration and do not limit this aspect.

[0028] The sophora alopecuroides extract composite biological pharmaceutical fertilizer of the present invention, the composite biological pharmaceutical fertilizer includes sophora alopecuroides total alkaloid preparation and superoxide dismutase powder; the sophora alopecuroides total alkaloid preparation and superoxide dismutase powder are compounded according to the mass ratio of active ingredients of 70-72:30-28.

[0029] In the embodiments of the present disclosure, the content of sophora alopecuroides total alkaloids in the sophora alopecuroides total biological preparation is 5%-6%.

[0030] In the embodiments of the present disclosure, the content of superoxide dismutase in the superoxide dismutase powder is 9000-11000 u / g.

[0031] In the embodiments of the present disclosure, the sophora alopecuroides total alkaloids are extracted from sophora alopecuroides by the cyclic percolation method; the cyclic percolation method extraction includes: the extraction solvent is ethanol with a mass percentage concentration of 66.7%, the flow rate of the extraction device is 3.69 m / s, the extraction time is 68 min, and the extraction temperature is 49.6 °C.

[0032] In the embodiments of the present disclosure, the sophora alopecuroides total alkaloid preparation is sophora alopecuroides total alkali wettable powder; the sophora alopecuroides total alkali wettable powder includes 5%-10% of sophora alopecuroides total alkaloid extract, 10% of sodium dodecylbenzenesulfonate, 1 (edited to correct the percentage to 15%)5% of dispersant NNO, and 65%-70% of bentonite.

[0033] The preparation method of the sophora alopecuroides extract composite biological pharmaceutical fertilizer of the present invention includes:

[0034] ① Using the cyclic percolation method to extract sophora alopecuroides total alkali;

[0035] ② Preparation of total alkaloids of Sophora alopecuroides preparation: According to the formula amount, use the total alkaloids of Sophora alopecuroides obtained in step ① to prepare wettable powder;

[0036] ③ Compound the superoxide dismutase powder and the wettable powder of total alkaloids of Sophora alopecuroides according to the mass ratio of active ingredients to prepare a compound biological fertilizer.

[0037] The application of the compound biological fertilizer of the Sophora alopecuroides extract of the present invention for preparing crop fertilizers.

[0038] In the embodiments of the present disclosure, the crops include peppers, cucumbers, and / or tomatoes, and the crop fertilizers are used to promote crop growth, increase production, improve quality, and / or increase stress resistance.

[0039] In the embodiments of the present disclosure, the crop fertilizer is used to control Botrytis cinerea of tomatoes.

[0040] The Sophora alopecuroides in the following examples is from Northwest A&F University or purchased through public market channels, and the superoxide dismutase powder is purchased through public market channels. For example, the superoxide dismutase powder with 9000-11000 u / g is purchased from Guangdong Huilong Biotechnology Co., Ltd.

[0041] Example 1 Extraction process of total alkaloids of Sophora alopecuroides

[0042] The Sophora alopecuroides was collected from Hotan area of Xinjiang, air-dried naturally and then crushed to a particle size of 4 mm. After sieving, it was stored in a dry place for later use. The extraction process of total alkaloids of Sophora alopecuroides was optimized by the response surface method:

[0043] Through the response surface experiment, the optimal process conditions for extracting total alkaloids of Sophora alopecuroides by the cyclic percolation extraction method were: ethanol content 66.7%, device flow rate 3.69 m / s, extraction time 68 min, temperature 49.6 °C. At this time, the yield of total alkaloids of Sophora alopecuroides was 3.20%.

[0044] Example 2 Preparation of 5% wettable powder of total alkaloids of Sophora alopecuroides

[0045] Through the performance test of the finished product, the formula of 5% wettable powder of total alkaloids of Sophora alopecuroides was obtained, as shown in Table 1 for details.

[0046] Table 1 Optimal formula of wettable powder of total alkaloids of Sophora alopecuroides

[0047]

[0048] The following compound biological fertilizers used in the examples are:

[0049] The 5% preparation of total alkaloids of Sophora alopecuroides prepared in Example 2 and superoxide dismutase powder with 10000 u / g;

[0050] Example 3: Screen the optimal ratio of the compound biological medicine fertilizer and apply the biological medicine fertilizer to the tomato pot experiment. The specific implementation process and method are as follows:

[0051] Transplant tomato seedlings into the test pots, with 1 plant planted in each pot; start the experiment when the tomatoes grow to 4 - 6 true leaves. Compound 5% total alkaloids of Sophora alopecuroides wettable powder and superoxide dismutase powder according to the mass ratio of the active ingredients to obtain 4 medicated fertilizer products with a final concentration of 125 mg·L ,

[0055] , , , , , * ,

[0057] , , * ,

[0056] ,

[0061] , , ,

[0054] ,

[0059] ,

[0053] , * ,

[0058] , -1 , , , , ,

[0060] , of the active ingredient, namely Biological Medicated Fertilizer 1 (total alkaloids of Sophora alopecuroides wettable powder: SOD powder = 1:0.2), Biological Medicated Fertilizer 2 (total alkaloids of Sophora alopecuroides wettable powder: SOD powder = 1:0.4), Biological Medicated Fertilizer 3 (total alkaloids of Sophora alopecuroides wettable powder: SOD powder = 1:0.8), Biological Medicated Fertilizer 4 (total alkaloids of Sophora alopecuroides wettable powder: SOD powder = 1:1.6). Use the biological stimulant Kelpu seaweed liquid as the medicated fertilizer control and the water treatment as the blank control, and conduct spray treatments respectively. Each treatment is repeated 10 times, spray the medicine once every 7 days, and spray continuously for 3 times. The liquid spraying amount each time is 7, 9, and 12 mL per plant in sequence, and the spraying time is selected at 9 am. Randomly sample at different times to measure the plant morphological indexes and related physiological and biochemical indexes.

[0052] Sample before each spraying. Randomly select 5 plants from each treatment and measure the stem diameter using a vernier caliper; measure the chlorophyll content, total phenol and flavonoid contents.

[0053] Table 2 Effects of biological medicated fertilizers with different ratios on the stem diameter of tomato seedlings

[0054]

[0055] Note: * Indicates a significant difference between the treatment and the control (p < 0.05).

[0056] Table 3 Effects of biological medicated fertilizers with different ratios on the chlorophyll content of tomato leaves

[0057]

[0058] Note: * Indicates a significant difference between the treatment and the control (p < 0.05).

[0059] Table 4 Effects of biological medicated fertilizers with different ratios on the total phenol content of tomato leaves Note: * Indicates a significant difference between the treatment and the control (p < 0.05).

[0060]

[0061] Table 5 Effects of biological medicated fertilizers with different ratios on the flavonoid content of tomato leaves

[0062]

[0063]

[0064] Note: * Indicates a significant difference between treatment and control (p < 0.05).

[0065] The test results are shown in Table 2-5. After applying the compound biological fertilizer, the growth indexes of tomato plants are higher than those of the control group. Among them, the application of compound biological fertilizer 2 (total alkaloids of Sophora alopecuroides preparation and superoxide dismutase powder in a mass ratio of effective components of 70:30) can significantly increase the net growth of tomato stem diameter, and the chlorophyll content, flavonoid content, and total phenol content of tomato leaves are all significantly increased. It shows that the compound biological fertilizer 2 has the effects of promoting the growth of tomatoes and increasing the stress resistance of tomatoes.

[0066] The compound biological fertilizer used in the following examples is as follows:

[0067] The 5% total alkaloids of Sophora alopecuroides preparation and superoxide dismutase powder (10,000 u / g) prepared in Example 2, and the total alkaloids of Sophora alopecuroides preparation and superoxide dismutase powder are in a mass ratio of effective components of 70:30.

[0068] Example 4 The above biological fertilizer was used in tomato production in a certain place in Shaanxi. The specific implementation process and methods are as follows:

[0069] The test was carried out at the experimental base of Caoxinzhuang Farm in Yangling District, Shaanxi Province. Two treatments were set in the test, with 3 replicates for each treatment, and arranged in a randomized block design; it was sprayed once at the budding stage, flowering stage, fruit setting stage, and fruit growth stage of tomatoes, for a total of 5 sprays. When spraying, a knapsack lever-type manual sprayer was used to spray on both the front and back sides of tomato leaves, and the spraying volume was 600 kg·hm -2 , During the investigation, random samples were taken in each plot at different investigation periods to measure the tomato fruit yield and fruit quality. The control treatment did not apply any fertilizer, and the field management was the same during the planting period.

[0070] Samples were taken before each spraying. 5 plants were randomly selected from each treatment, and a vernier caliper was used to measure the stem diameter; the fresh weight of tomato fruits was directly weighed, with 3 replicates. The soluble sugar content was determined by the anthrone method; the organic acid was determined by titration; the vitamin C content was determined by the 2,6-dichloroindophenol method; the chlorophyll content, total phenol, and flavonoid content were determined.

[0071] Table 6 Effects of biological fertilizer on tomato stem diameter

[0072]

[0073]

[0074] Note: * Indicates a significant difference between the treatment and the control (p < 0.05).

[0075] Table 7 Effects of biological bio-fertilizer on photosynthetic pigment content in tomato leaves

[0076]

[0077]

[0078] Note: * Indicates a significant difference between the treatment and the control (p < 0.05).

[0079] Table 8 Effects of biological bio-fertilizer on flavonoid content in tomato leaves

[0080]

[0081]

[0082] Note: * Indicates a significant difference between the treatment and the control (p < 0.05).

[0083] Table 9 Effects of biological bio-fertilizer on total phenol content in tomato leaves

[0084]

[0085] Note: * Indicates a significant difference between the treatment and the control (p < 0.05).

[0086] Table 10 Effects of compound biological bio-fertilizer on the quality of the first, second, and third clusters of tomatoes on average

[0087]

[0088] Note: * Indicates a significant difference between the treatment and the control (p < 0.05).

[0089] The test results are shown in Tables 6 - 10. After applying the compound biological bio-fertilizer, the growth indexes and quality of tomato plants are significantly higher than those of the control group. Among them, the net increase in tomato stem diameter, chlorophyll a content, flavonoid content, and total phenol content all increase significantly; the fruit setting rate, yield, soluble sugar content, organic acid content, and Vc content of tomato fruits all increase significantly. It shows that the compound biological bio-fertilizer has the effects of promoting the growth of tomatoes, increasing production, improving quality, and enhancing the stress resistance of tomatoes.

[0090] Example 4 The above compound biological bio-fertilizer was used to control tomato gray mold in a certain place in Shaanxi. The specific implementation process and methods are as follows:

[0091] The test crop was tomato, and the experiment was carried out in a greenhouse in Yangling, Shaanxi. This greenhouse had been growing tomatoes for many years, and when the tomato gray mold disease was severe, the incidence rate could reach over 80%. The tomato field was divided into 5 plots, and the total field area for each treatment was 3×6 = 18 m 2 ; For each treatment, 3 replicates were set, and the area of each replicate was 6 m 2 ; 30 tomato seedlings were planted in each treatment and managed conventionally. The experiment had treatments and controls. The controls were divided into a pyrimethanil control and a clear water blank control. For the treatments, the compound biological medicine fertilizer of the present invention and 5% total alkaloids of Sophora alopecuroides wettable powder were applied. A knapsack lever-type manual sprayer was used to spray on both the front and back sides of the tomato leaves, and the spraying liquid amount was 600 kg·hm -2 . The disease incidence was investigated 10 days after the last application of the medicine, and the incidence rate and disease prevention effect were calculated.

[0092] Leaf grading method: Grade 0, no disease spots; Grade 1, 3 disease spots on a single leaf; Grade 3, 4 - 6 disease spots on a single leaf; Grade 5, 7 - 10 disease spots on a single leaf; Grade 7, 11 - 20 disease spots on a single leaf; Grade 9, disease spots on a single leaf, dense, occupying more than 1 / 4 of the leaf area.

[0093] Disease index = [∑(number of diseased plants at each level × corresponding disease level) / (total number of investigated plants × highest investigated disease level)] × 100 Control effect (%) = [(control disease index - treatment disease index) / control disease index] × 100

[0094] The results are shown in Table 11.

[0095] Table 11 Control effect of the compound biological medicine fertilizer on tomato gray mold

[0096]

[0097] Note: There was a significant difference between the treatment and the control (p < 0.05).

[0098] As can be seen from Table 11 above, the compound biological medicine fertilizer of the present invention has the effects of disease prevention and growth promotion, can inhibit the occurrence of diseases, and can also improve the yield and quality of crops.

[0099] Although the present invention has been described in detail above with general descriptions and specific implementation examples, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A Sophora alopecuroides extract composite biological medicine fertilizer, characterized in that, The compound biological medicine fertilizer comprises a total alkaloid preparation of Sophora alopecuroides and a superoxide dismutase powder; The total alkaloid preparation of Sophora alopecuroides and the superoxide dismutase powder are compounded according to the mass ratio of active ingredients of 70-72:30-28.

2. The Sophora alopecuroides extract composite biological fertilizer according to claim 1, wherein The content of the total alkaloids of Sophora alopecuroides in the total biological preparation of Sophora alopecuroides is 5%-6%.

3. The Sophora alopecuroides extract composite biological fertilizer according to claim 1 or 2, characterized in that, The content of superoxide dismutase in the superoxide dismutase powder is 9000-11000 u / g.

4. The Sophora alopecuroides extract composite biological medicine fertilizer according to claim 1 or 2, characterized in that The total alkaloids of Sophora alopecuroides are extracted from Sophora alopecuroides by the cyclic percolation method; The cyclic percolation extraction includes: the extraction solvent is ethanol with a mass percentage concentration of 66.7%, the flow rate of the extraction device is 3.69 m / s, the extraction time is 68 min, and the extraction temperature is 49.6 °C.

5. The Sophora alopecuroides extract composite biological fertilizer according to claim 1 or 2, characterized in that The total alkaloid preparation of Sophora alopecuroides is a wettable powder of total alkaloids of Sophora alopecuroides; The wettable powder of total alkaloids of Sophora alopecuroides includes 5%-10% of the total alkaloid extract of Sophora alopecuroides, 10% of sodium dodecylbenzenesulfonate, 15% of dispersant NNO, and 65%-70% of bentonite.

6. The preparation method of the Sophora alopecuroides extract composite biological fertilizer and pesticide according to any one of claims 1-5, characterized in that, Including: ① The total alkaloids of Sophora alopecuroides are extracted by the cyclic percolation method; ② The preparation of the total alkaloid preparation of Sophora alopecuroides: according to the formula amount, the total alkaloids of Sophora alopecuroides obtained in step ① are used for the preparation of the wettable powder; ③ The superoxide dismutase powder and the wettable powder of total alkaloids of Sophora alopecuroides are compounded according to the mass ratio of active ingredients to prepare a compound biological medicine fertilizer.

7. The preparation method of the Sophora alopecuroides extract composite biological fertilizer according to claim 6, characterized in that, The cyclic percolation extraction includes: the extraction solvent is ethanol with a mass percentage concentration of 66.7%, the flow rate of the extraction device is 3.69 m / s, the extraction time is 68 min, and the extraction temperature is 49.6 °C.

8. The application of the Sophora alopecuroides extract compound biological medicine fertilizer according to any one of claims 1-5 in the preparation of crop fertilizers.

9. The application according to claim 8, characterized in that The crops include peppers, cucumbers and / or tomatoes, and the crop fertilizers are used to promote the growth, increase the yield, improve the quality and / or increase the stress resistance of crops.

10. The application according to claim 8, wherein The crop fertilizer is used to control Botrytis cinerea of tomatoes.

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