Composition for enhancing ATP enzyme activity and preparation method thereof

Abstract

The invention relates to the field of food, in particular to a composition for enhancing ATPase activity and a preparation method thereof. The invention provides a composition. The composition is prepared from the following raw materials or is prepared from the following raw materials: poria cocos, roses, fructus lycii, pericarpium citri reticulatae, mulberries, jujubes, radix polygonati officinalis, longan, rhizoma polygonati and donkey-hide gelatin. The composition can improve the glucose intake, enhance the ATP enzyme activity, quickly supplement ATP and provide enough energy for the body.

Description

Technical Field

[0001] The present invention relates to the field of food, and particularly to a composition for enhancing ATPase activity and a preparation method thereof. Background Art

[0002] In traditional Chinese medicine's understanding of the causes of aging, great importance is attached to the functions of the zang-fu organs and essence qi. Deficiency of kidney yang, weakness of the spleen and stomach, insufficiency of essence qi, etc. are all mainstream theories of aging in traditional Chinese medicine. Traditional Chinese medicine believes that the kidney is the innate foundation of the body, storing essence qi, and the prosperity and decline of the kidney affect the prosperity and decline of primordial qi and the strength of the biochemical function. In the discussion of "a woman reaching the age of 77" and "a man reaching the age of 88" in "Plain Questions: On the Innateness of Man", the natural law of the prosperity and decline of kidney qi is used to illustrate the relationship between the process of human growth, development, and aging and the innate foundation, thus indicating that the key to aging lies in the prosperity and decline of kidney qi. Traditional Chinese medicine believes that the spleen and stomach are the acquired foundation of the body, and all food and water enter the stomach, and all the zang-fu organs receive qi from the stomach. If the spleen and stomach are weak, food and water cannot be digested and absorbed, and the nutrients needed by the human body cannot be supplemented in time, which will affect the health of the body and accelerate aging. Essence qi is divided into congenital essence qi and acquired essence qi. "Huangdi Neijing" says: The congenital qi is the "primordial qi" and "kidney qi", which comes from the congenital essence in the kidney; the acquired essence qi is various refined substances transformed from food and water, which comes from after birth and is transformed by the spleen and stomach. The remaining part after the metabolic balance of the acquired essence qi will eventually be stored in the kidneys and become a part of the kidney essence.

[0003] The essence of aging is cell attenuation. An international top journal "Cell" published a research review titled "The hallmarks of aging", which attributed the causes of aging to 9 factors such as cell senescence, stem cell exhaustion, mitochondrial dysfunction, etc., which is the famous "nine-dimensional cell theory". This research introduced scientific anti-aging to the cellular level. The research also divided human aging into three age groups: after 25 years old, 30 - 40 years old, and over 40 years old, and considered that these three age groups are the most critical periods of aging in life, and artificial interference within these three age groups can slow down the aging speed.

[0004] Metabolism refers to the process of material and energy exchange between the body and the external environment, as well as the self-renewal of substances and energy within the organism. It is one of the most basic characteristics of life phenomena, including two aspects: material metabolism and energy metabolism. Through material metabolism, the body absorbs nutrients from the outside world, decomposes and absorbs them in the body, releases the chemical energy contained therein, and converts it into energy that can be utilized by tissues and cells to maintain the normal life activities of the body.

[0005] Mitochondria are important organelles in eukaryotic cells, the key sites of cellular oxidative metabolism, and important places where cells produce ATP. They are known as the "energy factories of cells", and their activity can directly reflect the metabolic capacity of cells. ATP, namely adenosine triphosphate, is a high-energy phosphate compound commonly present in various living cells. It is the direct energy source within cells and is often referred to as the "energy currency in cells". When ATP in cells is hydrolyzed under the action of ATPase, a large amount of energy can be released, providing power for the occurrence and progress of chemical reactions such as cell metabolism. When ATP is insufficient or the activity of ATPase is not high, the body cannot obtain sufficient energy, and our organ functions will decline, metabolism will slow down, motor function will decrease, and the accumulation of toxins will increase, accelerating human aging. To supplement ATP, we need to increase the activity of transport proteins while increasing the glucose intake; otherwise, glucose cannot enter the cells and be decomposed into ATP.

[0006] Currently, there are few studies on enhancing the activity of cellular ATPase or increasing the content of cellular ATP. Therefore, existing therapeutic products and technologies still need to be improved and developed. Summary of the Invention

[0007] Based on the research results of the aging mechanisms in traditional Chinese and Western medicine, and targeting female sub-health, this invention uses modern scientific research techniques to screen out single Chinese herbs with high activity from 10 high-quality herbs including rose, polygonatum, donkey-hide gelatin, wolfberry, tangerine peel, mulberry, Chinese date, polygonatum odoratum, longan, and poria cocos. It combines the traditional Chinese medicine aging theory with modern medical technology to develop a formula through qualitative and quantitative methods.

[0008] In some embodiments, the present invention provides a composition, characterized by comprising the following raw materials or products made based on the inclusion of the following raw materials: poria cocos, rose, wolfberry, tangerine peel, mulberry, Chinese date, polygonatum odoratum, longan, polygonatum, and donkey-hide gelatin.

[0009] In some embodiments, the present invention provides a composition, characterized in that the composition comprises the following components in parts by weight: 1 - 60 parts of poria cocos, 1 - 40 parts of rose, 1 - 40 parts of wolfberry, 1 - 40 parts of tangerine peel, 1 - 40 parts of mulberry, 1 - 40 parts of Chinese date, 1 - 40 parts of polygonatum odoratum, 1 - 30 parts of longan, 1 - 30 parts of polygonatum, and 1 - 20 parts of donkey-hide gelatin.

[0010] In some embodiments, the present invention provides a composition, characterized in that the composition comprises the following components in parts by weight: 1 - 60 parts of poria cocos, 1 - 40 parts of rose, 1 - 40 parts of wolfberry, 1 - 40 parts of tangerine peel, 1 - 40 parts of mulberry, 1 - 40 parts of Chinese date, 1 - 40 parts of polygonatum odoratum, 1 - 20 parts of longan, 1 - 20 parts of polygonatum, and 1 - 10 parts of donkey-hide gelatin.

[0011] In some embodiments, the composition comprises the following components in parts by weight: 3 - 60 parts of Poria cocos, 2 - 40 parts of rose, 2 - 40 parts of wolfberry fruit, 2 - 40 parts of dried tangerine peel, 2 - 40 parts of mulberry, 2 - 40 parts of Chinese date, 2 - 40 parts of polygonatum odoratum, 2 - 20 parts of longan, 2 - 20 parts of polygonatum sibiricum, and 1 - 10 parts of donkey-hide gelatin.

[0012] In some embodiments, the composition comprises the following components in parts by weight: 9 - 40 parts of Poria cocos, 6 - 25 parts of rose, 6 - 25 parts of wolfberry fruit, 6 - 25 parts of dried tangerine peel, 6 - 25 parts of mulberry, 6 - 25 parts of Chinese date, 6 - 25 parts of polygonatum odoratum, 4 - 15 parts of longan, 4 - 15 parts of polygonatum sibiricum, and 1 - 8 parts of donkey-hide gelatin.

[0013] In some embodiments, the composition comprises the following components in parts by weight: 15 - 30 parts of Poria cocos, 10 - 20 parts of rose, 10 - 20 parts of wolfberry fruit, 10 - 20 parts of dried tangerine peel, 10 - 20 parts of mulberry, 10 - 20 parts of Chinese date, 10 - 20 parts of polygonatum odoratum, 7 - 10 parts of longan, 7 - 10 parts of polygonatum sibiricum, and 3 - 5 parts of donkey-hide gelatin.

[0014] In some embodiments, the composition comprises the following components in parts by weight: 60 parts of Poria cocos, 40 parts of rose, 40 parts of wolfberry fruit, 40 parts of dried tangerine peel, 35 parts of mulberry, 35 parts of Chinese date, 35 parts of polygonatum odoratum, 20 parts of longan, 20 parts of polygonatum sibiricum, and 15 parts of donkey-hide gelatin.

[0015] In some embodiments, the composition comprises the following components in parts by weight: 15 parts of Poria cocos, 10 parts of rose, 10 parts of wolfberry fruit, 10 parts of dried tangerine peel, 10 parts of mulberry, 10 parts of Chinese date, 10 parts of polygonatum odoratum, 7 parts of longan, 7 parts of polygonatum sibiricum, and 3 parts of donkey-hide gelatin.

[0016] In some embodiments, the composition comprises the following components in parts by weight: 9 parts of Poria cocos, 2 parts of rose, 2 parts of wolfberry fruit, 2 parts of dried tangerine peel, 2 parts of mulberry, 2 parts of Chinese date, 2 parts of polygonatum odoratum, 2 parts of longan, 2 parts of polygonatum sibiricum, and 2 parts of donkey-hide gelatin.

[0017] In some embodiments, the composition comprises the following components in parts by weight: 40 parts of Poria cocos, 25 parts of rose, 25 parts of wolfberry fruit, 25 parts of dried tangerine peel, 25 parts of mulberry, 25 parts of Chinese date, 25 parts of polygonatum odoratum, 15 parts of longan, 15 parts of polygonatum sibiricum, and 8 parts of donkey-hide gelatin.

[0018] In some embodiments, the composition comprises the following components in parts by weight: 30 parts of Poria cocos, 20 parts of rose, 20 parts of wolfberry fruit, 20 parts of dried tangerine peel, 20 parts of mulberry, 20 parts of Chinese date, 20 parts of polygonatum odoratum, 10 parts of longan, 10 parts of polygonatum sibiricum, and 5 parts of donkey-hide gelatin.

[0019] In some embodiments, the composition comprises the following components in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry fruit, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of Chinese date, 30 parts of polygonatum odoratum, 30 parts of longan, 30 parts of polygonatum sibiricum, and 10 parts of donkey-hide gelatin.

[0020] In some embodiments, the composition comprises the following components in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry fruit, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of Chinese date, 30 parts of polygonatum odoratum, 10 parts of longan, 10 parts of polygonatum sibiricum, and 5 parts of donkey-hide gelatin.

[0021] In some embodiments, the composition comprises the following components in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry fruit, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of Chinese date, 30 parts of polygonatum odoratum, 30 parts of longan, 30 parts of polygonatum sibiricum, and 15 parts of donkey-hide gelatin.

[0022] In some embodiments, the present invention provides a finished product, which is characterized by comprising the above composition and acceptable excipients;

[0023] Preferably, the finished product is a drug, a health food, a food, an external health product, an additive or an intermediate product;

[0024] Preferably, the finished product is a paste-like food;

[0025] Preferably, the finished product is used to increase glucose uptake, enhance ATPase activity, and increase cellular ATP content.

[0026] In some embodiments, a preparation method of the above composition comprises the following steps: (1) Select raw materials in one or several formulas, soak them with 5-30 times the amount of solvent for 10 min to 5 h respectively, then heat up for extraction 1-4 times, 10 min to 5 h each time, to obtain an extraction solution; (2) Combine the extraction solutions and concentrate them to a thick paste; (3) Dissolve an appropriate amount of donkey-hide gelatin in water, and mix it with the above thick paste;

[0027] Preferably, the method comprises the following steps: (1) Select raw materials in one or several formulas, soak them with 5-30 times the amount of solvent for 15 min to 3 h respectively, then heat up for extraction 1-4 times, 15 min to 3 h each time, to obtain an extraction solution; (2) Combine the extraction solutions and concentrate them to a thick paste; (3) Dissolve an appropriate amount of donkey-hide gelatin in water, and mix it with the above thick paste;

[0028] Preferably, the method comprises the following steps: Collect rose floral water, and in step (3), dissolve an appropriate amount of donkey-hide gelatin in water, and mix it with the above thick paste and rose floral water.

[0029] In some embodiments, the extraction is carried out by water extraction and membrane separation, decoction, reflux extraction, microwave extraction, ultrasonic extraction, maceration, dynamic extraction, and / or vacuum extraction;

[0030] In some embodiments, the solvent is selected from: water, ethanol or methanol. The water is selected from: deionized water, pure water, distilled water, etc.

[0031] In some embodiments, the temperature range for heating is 45 to 100 °C, preferably 50 to 90 °C, preferably 55 to 85 °C, preferably 60 to 80 °C.

[0032] The water extraction and membrane separation method includes two steps: water extraction and membrane separation. Water extraction refers to the process of placing the medicinal materials in a suitable decocting device, adding an appropriate proportion of water, boiling, maintaining a gentle boil for a period of time, and separating the decocted liquid. Membrane separation refers to a technique that selectively separates an aqueous extract containing molecules of different particle sizes through a semi-permeable membrane at the molecular level. In some embodiments, membrane separation includes tubular membrane separation and RO membrane separation steps.

[0033] The decoction method is to pre-treat the medicinal materials, add water and heat to boil, so that the active ingredients in the medicinal materials are fully extracted. Decoct like this 1 - 5 times, collect the decocted liquid each time, precipitate or filter, and then further evaporate and concentrate the supernatant to a certain concentration.

[0034] The reflux extraction method refers to when using an organic solvent to heat and extract the active ingredients of traditional Chinese medicine, in order to prevent the volatilization loss of the solvent, a condensation reflux device is used to improve the extraction efficiency.

[0035] The microwave extraction method uses high-frequency electromagnetic waves to penetrate the extraction medium and reach the inside of the material. In this process, the microwave energy is quickly converted into heat energy, causing the temperature inside the material cells to rise rapidly. When the pressure inside the cells exceeds the bearing capacity of the cells, the cells will rupture, and the active ingredients will flow out of the cells and dissolve in the extraction medium at a lower temperature, and then the extract is obtained through further filtration and separation.

[0036] The ultrasonic extraction method uses the mechanical effect, cavitation effect and thermal effect of ultrasonic waves to extract the active ingredients by increasing the movement speed of the medium molecules and the penetration power of the medium.

[0037] The maceration method belongs to a type of extraction. It uses the principle of similar solubility to dissolve the medicinal materials with a liquid solvent to extract the active ingredients in the medicinal materials.

[0038] The vacuum extraction method uses the principle that the boiling point of the solvent decreases under a vacuum state. The medicinal materials and the extraction solvent are in a certain proportion, and in a vacuum environment and at a suitable temperature, the active ingredients in the medicinal materials are extracted.

[0039] The dynamic extraction method refers to an extraction method in which the medicinal materials and the solvent move relative to each other in the leaching container and are continuously and fully contacted for extraction. In this way, natural substances are extracted in a mild dynamic environment, with a lower heating temperature and less damage to the active ingredients, resulting in less impurities in the solution and belonging to continuous production.

[0040] In some embodiments, a preparation method for preparing the above-mentioned finished product is characterized by including adding acceptable excipients to the product of step (3) for preparation;

[0041] Preferably, the finished product is a drug, a health food, a food, an external health product, an additive or an intermediate product;

[0042] Preferably, the finished product is a paste-like food;

[0043] Preferably, the finished product is used to increase glucose uptake, enhance ATPase activity, and increase the ATP content in cells.

[0044] In some embodiments, the above-mentioned composition or finished product is used in the preparation of products for preventing, treating or assisting in treating products that increase glucose uptake, enhance ATPase activity, and increase the ATP content in cells.

[0045] In some embodiments, the above-mentioned composition or finished product can delay female aging.

[0046] In some embodiments, the product is a drug, a health food, a food, an external health product, an additive or an intermediate product;

[0047] In some embodiments, the finished product is a paste-like food.

[0048] The present invention uses top-quality medicinal materials that are both medicine and food homology, tonifying the kidney and filling essence, strengthening the spleen and supplementing qi, and nourishing blood and calming the mind. According to the modern medical aging mechanism, the present invention uses modern molecular biology techniques and a scientific formula developed by qualitative and quantitative methods, which can supplement a large amount of ATP in a short period of time, activate the activity of ATPase, release a large amount of energy, provide energy for cell activities, metabolism, etc., and delay the occurrence of aging symptoms. It is found through modern scientific research equipment that after consumption, it can not only provide a high glucose uptake, enhance ATPase activity, quickly supplement ATP, and provide sufficient energy for the body. Detailed implementation manners

[0049] The technical solutions of the present invention are further described below through specific embodiments. The specific embodiments do not represent a limitation on the protection scope of the present invention. Some non-essential modifications and adjustments made by others based on the concept of the present invention still fall within the protection scope of the present invention.

[0050] As used herein, the term "comprising" means that the compositions and methods include the recited elements, but do not exclude others. When used to define compositions and methods, "consisting essentially of" means excluding other elements essential to the combination. For example, as defined herein, a composition consisting essentially of certain elements does not exclude other elements that do not materially affect the basic and novel characteristics of the claimed invention. "Consisting of" means excluding other components and method steps beyond the recited measure that are essential. The embodiments defined by each transitional term are within the scope of the present invention.

[0051] Unless otherwise indicated, as used herein, the singular forms "a", "an", and "the" include plural instances. Thus, reference to "a composition" also includes its plural "some compositions".

[0052] The term "extract" refers to an extract obtained by extracting a raw material using water or an organic solvent.

[0053] The term "extract combination" refers to both a mixture of extracts obtained by separately extracting the raw materials of each, and an extract obtained by extracting a mixture of raw materials.

[0054] The term "composition" may refer to a single compound, or may refer to a combination of at least two compounds. For example, a composition may contain an active ingredient extracted from a raw material and an acceptable excipient.

[0055] "Acceptable excipients" are diluents, adjuvants, excipients or vehicles administered with the composition. Excipients include any and all physiologically compatible solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic agents and absorption delaying agents, etc. Examples of excipients include, but are not limited to, sterile liquids such as water, oils and lipids such as phospholipids and glycolipids. These sterile liquids include, but are not limited to, those derived from petroleum, animal, vegetable or synthetic sources such as peanut oil, soybean oil, mineral oil, sesame oil, etc.

[0056] "Treatment" of a patient's disease means: preventing the disease from occurring in a patient who is predisposed or has not yet manifested symptoms of the disease; or inhibiting the disease or preventing its development; or alleviating the disease or causing its regression.

[0057] The compositions according to the present invention can be used as nutritional products, health products or drugs. The term "health food" means any product having nutritional and / or physiological effects, especially including food supplements, foods, diet products, etc. These products can be administered particularly via oral, gastric or intravenous routes, but are not limited thereto.

[0058] Each raw material in the disclosed composition is a commonly used medicinal and edible homologous Chinese herbal medicine, which is detailedly recorded in "Chinese Pharmacopoeia" and "Compendium of Chinese Materia Medica", etc., and can be easily obtained through commercial channels. The present invention has no special restrictions on the origin of these Chinese herbal medicines, etc., as long as they meet relevant national standards or regulations.

[0059] Poria (scientific name or official name), English name Indian Buead Tuckahoe, Latin name Poria.

[0060] Rose (scientific name or official name), English name Rose, Latin name Rosa Rugosa.

[0061] Lycium barbarum fruit (scientific name or official name), English name Babury Wolfberry Fruit, Latin name Lycii Fructus.

[0062] Tangerine peel (scientific name or official name), English name Pericarpium Citri Reticulatae, Latin name Citrus Reticulata Blanco.

[0063] Mulberry fruit (scientific name or official name), English name Mulberry Fruit, Latin name Fructus Mori.

[0064] Chinese date (scientific name or official name), English name Jujube, Latin name Ziziphus Jujuba Mill.

[0065] Polygonatum odoratum rhizome (scientific name or official name), English name Fragrant Solomonseal Rhizome, Latin name Rhizoma Polygonati Odorati.

[0066] Dried longan pulp (scientific name or official name), English name Dried Longan Pulp, Latin name Longan Arillus.

[0067] Polygonatum sibiricum (scientific name or official name), English name Siberia Landpick, Latin name Polygonatum sibiricum Delarb ex Redoute.

[0068] Donkey-hide gelatin (scientific name or official name), English name Donkey-hide Gelatin, Latin name Colla Corii Asini.

[0069] Formulation of the extract combination in Example 1 and its preparation method

[0070] A traditional Chinese medicine extract combination, which is extracted from the following combination of traditional Chinese medicines by weight:

[0071] 60 parts of Poria cocos, 40 parts of rose flowers, 40 parts of wolfberries, 40 parts of dried tangerine peel, 35 parts of mulberries, 35 parts of Chinese dates, 35 parts of polygonatum odoratum, 20 parts of longans, 20 parts of polygonatum sibiricum, and 15 parts of donkey-hide gelatin.

[0072] (1) Soak Poria cocos, wolfberries, mulberries, Chinese dates, polygonatum odoratum, longans, polygonatum sibiricum, and dried tangerine peel for 30 minutes, add 25 times the amount of water, and decoct for 4 hours;

[0073] (2) Extract rose flowers once, add 10 times the amount of water, soak for 60 minutes, extract under reduced pressure at 70°C for 30 minutes, and collect the aromatic water at the same time;

[0074] (3) Combine the extraction solutions and concentrate under reduced pressure at 65°C to a thick paste;

[0075] (4) Dissolve donkey-hide gelatin in an appropriate amount of water, mix it with the above thick paste and rose flower aromatic water, and adjust to an appropriate concentration.

[0076] Formula and preparation method of the extract combination in Example 2

[0077] A traditional Chinese medicine extract combination, which is extracted from the following combination of traditional Chinese medicines by weight:

[0078] 15 parts of Poria cocos, 10 parts of rose flowers, 10 parts of wolfberries, 10 parts of dried tangerine peel, 10 parts of mulberries, 10 parts of Chinese dates, 10 parts of polygonatum odoratum, 7 parts of longans, 7 parts of polygonatum sibiricum, and 3 parts of donkey-hide gelatin.

[0079] (1) Soak Poria cocos, wolfberries, mulberries, Chinese dates, polygonatum odoratum, longans, polygonatum sibiricum, and dried tangerine peel for 2 hours, add 18 times the amount of water, and reflux for 4 hours;

[0080] (2) Extract rose flowers once, add 15 times the amount of water, soak for 30 minutes, extract under reduced pressure at 60°C for 1 hour, and collect the aromatic water at the same time;

[0081] (3) Combine the extraction solutions and concentrate under reduced pressure at 60°C to a thick paste;

[0082] (4) Dissolve donkey-hide gelatin in an appropriate amount of water, mix it with the above thick paste and rose flower aromatic water, and adjust to an appropriate concentration.

[0083] Formula and preparation method of the extract combination in Example 3

[0084] A traditional Chinese medicine extract combination, which is extracted from the following combination of traditional Chinese medicines by weight:

[0085] 9 parts of Poria cocos, 2 parts of rose flowers, 2 parts of wolfberries, 2 parts of dried tangerine peel, 2 parts of mulberries, 2 parts of Chinese dates, 2 parts of polygonatum odoratum, 2 parts of longans, 2 parts of polygonatum sibiricum, and 2 parts of donkey-hide gelatin.

[0086] (1) Poria cocos, wolfberry fruit, tangerine peel, mulberry, Chinese date, polygonatum odoratum, longan are extracted by ultrasonic wave at 60°C three times with 8 times the amount of water each time. The first extraction is for 1 hour, and the latter two extractions are for 45 minutes each time;

[0087] (2) Polygonatum sibiricum is extracted once with 10 times the amount of water at 80°C for 1 hour;

[0088] (3) Rose flower is extracted once with 15 times the amount of water under reduced pressure at 80°C for 1 hour, and the aromatic water is collected simultaneously;

[0089] (4) The extraction solutions are combined and concentrated under reduced pressure to a thick paste at 70°C;

[0090] (5) Colla corii asini is melted with an appropriate amount of water, mixed with the above thick paste and rose flower aromatic water, and adjusted to an appropriate concentration.

[0091] Formulation and preparation method of the extract combination in Example 4

[0092] A traditional Chinese medicine extract combination is composed of the extraction of traditional Chinese medicinal materials in the following parts by weight:

[0093] 40 parts of Poria cocos, 25 parts of rose flower, 25 parts of wolfberry fruit, 25 parts of tangerine peel, 25 parts of mulberry, 25 parts of Chinese date, 25 parts of polygonatum odoratum, 15 parts of longan, 15 parts of Polygonatum sibiricum and 8 parts of Colla corii asini.

[0094] (1) Poria cocos, rose flower, wolfberry fruit, tangerine peel, mulberry, Chinese date, polygonatum odoratum, longan, Polygonatum sibiricum are extracted under reduced pressure at 80°C twice with 12 times the amount of water each time for 1 hour each time, and concentrated to a thick paste under reduced pressure at 70°C;

[0095] (2) Colla corii asini is melted with an appropriate amount of water, mixed with the above thick paste, and adjusted to an appropriate concentration.

[0096] Formulation and preparation method of the extract combination in Example 5

[0097] A traditional Chinese medicine extract combination is composed of the extraction of traditional Chinese medicinal materials in the following parts by weight:

[0098] 30 parts of Poria cocos, 20 parts of rose flower, 20 parts of wolfberry fruit, 20 parts of tangerine peel, 20 parts of mulberry, 20 parts of Chinese date, 20 parts of polygonatum odoratum, 10 parts of longan, 10 parts of Polygonatum sibiricum, 5 parts of Colla corii asini.

[0099] (1) Poria cocos, wolfberry fruit, mulberry, Chinese date, polygonatum odoratum, longan, Polygonatum sibiricum are soaked for 30 minutes, and extracted twice with water at 100°C. The first time is with 10 times the amount of water, and the second time is with 8 times the amount of water, each time for 1 hour;

[0100] (2) Tangerine peel is extracted once, soaked with 15 times the amount of water for 60 minutes, and extracted at 80°C for 30 minutes;

[0101] (3) Extract the rose once, add 15 times the amount of water and soak for 60 min, extract under reduced pressure at 80 °C for 30 min, and collect the aromatic water at the same time;

[0102] (4) Combine the extracts and concentrate them under reduced pressure at 60 °C to a thick paste;

[0103] (5) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the above thick paste and the rose aromatic water, and adjust to the appropriate concentration.

[0104] Formula and preparation method of the extract combination in Example 6

[0105] A traditional Chinese medicine extract combination is composed of the extraction of traditional Chinese medicinal materials in the following parts by weight:

[0106] Poria cocos 30 parts, rose 30 parts, wolfberry fruit 30 parts, tangerine peel 30 parts, mulberry 30 parts, Chinese date 30 parts, polygonatum odoratum 30 parts, longan 30 parts, polygonatum sibiricum 30 parts, donkey-hide gelatin 10 parts.

[0107] (1) Decoct and extract Poria cocos, wolfberry fruit, mulberry, Chinese date, polygonatum odoratum, longan, and polygonatum sibiricum twice with water. The first time uses 15 times the amount of water, and the second time uses 10 times the amount of water, each time for 1 hour;

[0108] (2) Extract the tangerine peel once, add 10 times the amount of water and soak for 60 min, extract at 80 °C for 30 min;

[0109] (3) Extract the rose once, add 10 times the amount of water and soak for 60 min, extract under reduced pressure at 80 °C for 30 min, and collect the aromatic water at the same time;

[0110] (4) Combine the extracts and concentrate them under reduced pressure at 65 °C to a thick paste;

[0111] (5) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the above thick paste and the rose aromatic water, and adjust to the appropriate concentration.

[0112] Formula and preparation method of the extract combination in Example 7

[0113] A traditional Chinese medicine extract combination is composed of the extraction of traditional Chinese medicinal materials in the following parts by weight:

[0114] Poria cocos 30 parts, rose 30 parts, wolfberry fruit 30 parts, tangerine peel 30 parts, mulberry 30 parts, Chinese date 30 parts, polygonatum odoratum 30 parts, longan 10 parts, polygonatum sibiricum 10 parts, donkey-hide gelatin 5 parts.

[0115] (1) Soak Poria cocos, wolfberry fruit, tangerine peel, mulberry, Chinese date, polygonatum odoratum, longan, and polygonatum sibiricum in 20 times the amount of water for 60 min, and extract at 80 °C for 1 h;

[0116] (2) Extract the rose once, add 10 times the amount of water and soak for 60 min, extract under reduced pressure at 70 °C for 45 min, and collect the aromatic water at the same time;

[0117] (3) Combine the extraction solution and concentrate it under reduced pressure at 55 °C to a thick paste.

[0118] (4) Dissolve donkey-hide gelatin in an appropriate amount of water, mix it evenly with the above-mentioned thick paste and rose aromatic water, and adjust to an appropriate concentration.

[0119] Formula and preparation method of the extract combination in Example 8

[0120] A traditional Chinese medicine extract combination is composed of the following traditional Chinese medicines combined and extracted by weight:

[0121] Poria cocos 30 parts, rose 30 parts, wolfberry fruit 30 parts, tangerine peel 30 parts, mulberry 30 parts, Chinese date 30 parts, polygonatum odoratum 30 parts, longan 30 parts, polygonatum sibiricum 30 parts, donkey-hide gelatin 15 parts.

[0122] (1) Soak Poria cocos, wolfberry fruit, tangerine peel, mulberry, Chinese date, polygonatum odoratum, longan, and polygonatum sibiricum in 18 times the amount of water for 60 min, and extract under reduced pressure at 80 °C for 1.5 h.

[0123] (2) Extract rose once, soak it in 12 times the amount of water for 10 min, extract under reduced pressure at 65 °C for 1 h, and collect the aromatic water at the same time.

[0124] (3) Combine the extraction solution and concentrate it under reduced pressure at 65 °C to a thick paste.

[0125] (4) Dissolve donkey-hide gelatin in an appropriate amount of water, mix it evenly with the above-mentioned thick paste and rose aromatic water, and adjust to an appropriate concentration.

[0126] Effect test

[0127] 1. Experimental model:

[0128] Rat cardiomyocyte line (H9c2, American Type Culture Collection), human hepatocellular carcinoma cell line (HepG2, American Type Culture Collection) and mouse myoblast cell line (C2C12, American Type Culture Collection)

[0129] 2. Experimental reagents:

[0130] DMEM high glucose medium (dulbecco's modified eagle medium, Invitrogen, Carlsbad, CA), fetal bovine serum (Fetal bovine serum, Invitrogen, Carlsbad, CA), bovine serum albumin (Bovineserum albumin, USB, Cleveland, Ohio, USA), trypsin-EDTA (Trypsin-EDTA, Invitrogen, Carlsbad, CA), glucose test kit (Beijing Pulilai).

[0131] 3. Experimental instruments:

[0132] Microplate Reader (Dynex Opsys MR TM ),Victor V3 Multi-label Counter (Perkin Elmer, Turku, Finland)

[0133] 4. Experiments:

[0134] (1) Cell culture: After resuscitation, H9c2 cells, HepG2 cells and C2C12 cells were transferred to culture dishes with DMEM high glucose medium containing 10% fetal bovine serum and cultured in a constant temperature incubator at 37°C and 5% CO2. After the cells adhered and grew confluently, they were digested with trypsin. H9c2 cells were passaged once every three days at a ratio of 1:4, HepG2 cells were passaged once every 3 days at a ratio of 1:4, and C2C12 cells were passaged once every 3 days at a ratio of 1:10. Cells in the logarithmic growth phase were used for the experiment.

[0135] (2) Determination of in-situ ATP production (ATP-GC in situ): Logarithmic growth phase H9c2 cells were seeded in 24-well cell culture plates at a density of 25,000 cells / well, and normal cell control groups and test sample treatment groups were set up respectively. After the cells were stably adherent and grew for 24 h, fresh cell culture medium containing 3 and 10 mg / mL of the test article was added and incubated in a constant temperature incubator at 37°C and 5% CO2 for a certain period of time. The amount of ATP in the cells in situ and the amount of ATP produced within 0 - 15 min were measured by bioluminescence assay, and the untreated cells were used as the control group for normalization, and the area under the curve (AUC) was calculated.

[0136] The changes in intracellular ATP levels within 15 minutes after the composition stimulated H9c2 cells for 2, 6, and 24 hours are shown in Table 1.

[0137] Table 1. Effects of the test article on ATP-GC of H9c2 cells

[0138]

[0139] Control AUC2 value = 750.0 ± 13.2; *: p < 0.05.

[0140] The experimental results showed that, compared with the normal control group, the use of the composition at 10 mg / mL significantly increased the production of ATP by about 30% after 24 hours of action, enhanced the energy of the cells, and was beneficial to the production of "Qi" in the human body.

[0141] (3) In-situ antioxidant capacity determination (GSH): After treating H9c2 cells with the drug for a certain time, they were seeded in a 12-well cell culture plate at a cell density of 37,500 cells / well, and the normal cell control group and the test sample treatment group were set up respectively. After the cells were stably adherent and grew for 24 hours, the cell culture medium containing 10 mg / mL of the test article newly prepared was added, and they were placed in a constant temperature incubator at 37°C and 5% CO2 and incubated for a certain time respectively. The intracellular GSH level was measured by the Griffith method.

[0142] The experimental results showed that the intracellular GSH in H9c2 cells increased by about 20% (p = 0.005) after being stimulated with the drug (10 mg / mL) for 24 hours, indicating that the traditional Chinese medicine composition could significantly improve the antioxidant capacity of the cells.

[0143] (4) ATP level determination: Logarithmic growth phase HepG2 cells were seeded in a 96-well cell culture plate at a cell density of 5000 cells / well, and the normal cell control group and the test sample treatment group were set up respectively. After the cells were stably adherent and grew for 24 hours, the cell culture medium containing 0.3 and 1 mg / mL of the test article newly prepared was added, and they were placed in a constant temperature incubator at 37°C and 5% CO2 and incubated for 2 and 6 hours respectively.

[0144] The changes in the intracellular ATP level in HepG2 cells after the composition stimulated them for 2 and 6 hours are shown in Table 2.

[0145] The changes in the intracellular ATP hydrolase activity in HepG2 cells after the composition stimulated them for 2 and 6 hours are shown in Table 3.

[0146] Table 2. Effects of the test article on ATP in HepG2 cells (2 and 6 hours)

[0147]

[0148] Table 3. Effects of the test article on ATP hydrolase activity in HepG2 cells (2 and 6 hours)

[0149]

[0150] The experimental results showed that, compared with the normal control group, the extracts at 0.3 and 1 mg / mL stimulated HepG2 cells for 6 hours, which could promote the ATP production of the cells by about 25% and 30%, respectively. Moreover, the extract could significantly reduce the activity of hydrolase in HepG2 cells, indicating that this composition could promote the ATP production of HepG2 cells and inhibit the hydrolysis of ATP in HepG2 cells.

[0151] (5) C2C12: Logarithmic growth phase C2C12 cells were inoculated into 96-well cell culture plates at a cell density of 8,000 cells / well, and the normal cell control group and the test sample treatment group were set up respectively. After the cells were stably adherent and grew for 24 h, the newly prepared cell culture media containing 0.3 and 1 mg / mL of the test article were added, and they were placed in a constant temperature incubator at 37 °C and 5% CO2 and incubated for 4 and 6 h respectively.

[0152] The changes in the intracellular ATP levels of the composition stimulating C2C12 cells for 4 and 6 hours are shown in Table 4.

[0153] Table 4. Effects of the test article on ATP in C2C12 cells (4 and 6 hours)

[0154]

[0155] The experimental results showed that, compared with the normal control group, the extracts at 0.3 and 1 mg / mL stimulated C2C12 cells for 6 hours, which could promote the ATP production of these two kinds of cells by about 10% and 27%, respectively. It indicated that this traditional Chinese medicine composition could promote the ATP production of C2C12 cells.

[0156] (6) Determination of glucose uptake level: Logarithmic growth phase HepG2 cells and C2C12 cells were inoculated into 96-well cell culture plates at cell densities of 5,000 cells / well and 8,000 cells / well respectively. The cells were divided into blank (cell-free) group, normal cell control group and test sample treatment group, with 3 replicate wells in each group. They were placed in a constant temperature incubator at 37 °C and 5% CO2. After adding the drug for 24 h, the cell culture media were removed to measure the glucose content, and the glucose uptake of the cells was calculated. The glucose content in the supernatant of the cell culture media was measured by the glucose oxidase method (glucose test kit), and the glucose uptake of each well was obtained by subtracting the measured glucose content in the cell culture media from the average glucose content of the blank group without inoculated cells.

[0157] The changes in the cell glucose uptake levels of the composition stimulating C2C12 cells for 2, 4 and 6 hours are shown in Table 5.

[0158] The changes in the cell glucose uptake levels of the composition stimulating HepG2 cells for 2, 4 and 6 hours are shown in Table 6.

[0159] Table 5. Effects of the test article on glucose uptake in C2C12 cells (2, 4, and 6 hours)

[0160]

[0161]

[0162] Table 6. Effects of the test article on glucose uptake in HepG2 cells (2, 4, and 6 hours)

[0163]

[0164] The experimental results showed that in the glucose uptake assay, treatment with the composition could promote the glucose uptake levels in C2C12 cells and HepG2 cells, improve the sugar uptake ability of the cells, and promote the cells to metabolize and produce more energy.

[0165] Note: The test article added to the test sample treatment group was prepared in Example 5.

[0166] Statistical method: Statistical analysis was performed using SPSS 22.0. The level of statistical significance was set at P ≤ 0.05. Measurement data were expressed as mean ± standard deviation, and one-way analysis of variance (ANOVA) test was used for statistical analysis. Statistical differences and biological significance were considered during evaluation.

Claims

1. A composition, characterized in that, Comprising the following raw materials or products made based on the inclusion of the following raw materials: Poria cocos, rose flower, wolfberry fruit, dried tangerine peel, mulberry, Chinese date, polygonatum odoratum, longan, polygonatum sibiricum, donkey-hide gelatin.

2. The composition according to claim 1, wherein The composition comprises the following components in parts by weight: 1 - 60 parts of Poria cocos, 1 - 40 parts of rose flower, 1 - 40 parts of wolfberry fruit, 1 - 40 parts of dried tangerine peel, 1 - 40 parts of mulberry, 1 - 40 parts of Chinese date, 1 - 40 parts of polygonatum odoratum, 1 - 30 parts of longan, 1 - 30 parts of polygonatum sibiricum, and 1 - 20 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 1 - 60 parts of Poria cocos, 1 - 40 parts of rose flower, 1 - 40 parts of wolfberry fruit, 1 - 40 parts of dried tangerine peel, 1 - 40 parts of mulberry, 1 - 40 parts of Chinese date, 1 - 40 parts of polygonatum odoratum, 1 - 20 parts of longan, 1 - 20 parts of polygonatum sibiricum, and 1 - 10 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 3 - 60 parts of Poria cocos, 2 - 40 parts of rose flower, 2 - 40 parts of wolfberry fruit, 2 - 40 parts of dried tangerine peel, 2 - 40 parts of mulberry, 2 - 40 parts of Chinese date, 2 - 40 parts of polygonatum odoratum, 2 - 20 parts of longan, 2 - 20 parts of polygonatum sibiricum, and 1 - 10 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 9 - 40 parts of Poria cocos, 6 - 25 parts of rose flower, 6 - 25 parts of wolfberry fruit, 6 - 25 parts of dried tangerine peel, 6 - 25 parts of mulberry, 6 - 25 parts of Chinese date, 6 - 25 parts of polygonatum odoratum, 4 - 15 parts of longan, 4 - 15 parts of polygonatum sibiricum, and 1 - 8 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 15 - 30 parts of Poria cocos, 10 - 20 parts of rose flower, 10 - 20 parts of wolfberry fruit, 10 - 20 parts of dried tangerine peel, 10 - 20 parts of mulberry, 10 - 20 parts of Chinese date, 10 - 20 parts of polygonatum odoratum, 7 - 10 parts of longan, 7 - 10 parts of polygonatum sibiricum, and 3 - 5 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 60 parts of Poria cocos, 40 parts of rose flower, 40 parts of wolfberry fruit, 40 parts of dried tangerine peel, 35 parts of mulberry, 35 parts of Chinese date, 35 parts of polygonatum odoratum, 20 parts of longan, 20 parts of polygonatum sibiricum, and 15 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 15 parts of Poria cocos, 10 parts of rose flower, 10 parts of wolfberry fruit, 10 parts of dried tangerine peel, 10 parts of mulberry, 10 parts of Chinese date, 10 parts of polygonatum odoratum, 7 parts of longan, 7 parts of polygonatum sibiricum, and 3 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 9 parts of Poria cocos, 2 parts of rose flower, 2 parts of wolfberry fruit, 2 parts of dried tangerine peel, 2 parts of mulberry, 2 parts of Chinese date, 2 parts of polygonatum odoratum, 2 parts of longan, 2 parts of polygonatum sibiricum, and 2 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 40 parts of Poria cocos, 25 parts of rose flower, 25 parts of wolfberry fruit, 25 parts of dried tangerine peel, 25 parts of mulberry, 25 parts of Chinese date, 25 parts of polygonatum odoratum, 15 parts of longan, 15 parts of polygonatum sibiricum, and 8 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 30 parts of Poria cocos, 20 parts of rose flower, 20 parts of wolfberry fruit, 20 parts of dried tangerine peel, 20 parts of mulberry, 20 parts of Chinese date, 20 parts of polygonatum odoratum, 10 parts of longan, 10 parts of polygonatum sibiricum, and 5 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry fruit, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of Chinese date, 30 parts of polygonatum odoratum, 30 parts of longan, 30 parts of polygonatum sibiricum, and 10 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry fruit, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of Chinese date, 30 parts of polygonatum odoratum, 10 parts of longan, 10 parts of polygonatum sibiricum, and 5 parts of donkey-hide gelatin; Preferably, the composition comprises the following components in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry fruit, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of Chinese date, 30 parts of polygonatum odoratum, 30 parts of longan, 30 parts of polygonatum sibiricum, and 15 parts of donkey-hide gelatin.

3. A manufactured product, characterized in that, Comprising the composition according to any one of claims 1-2 and an acceptable excipient; Preferably, the finished product is a drug, health food, food, external health product, additive or intermediate product; Preferably, the finished product is a paste-like food; Preferably, the finished product is used to increase glucose uptake, enhance ATPase activity, and increase cellular ATP content.

4. A preparation method of the composition according to any one of claims 1-2, comprising the following steps: (1) Select the raw materials in one or several formulas, soak them in 5-30 times the amount of solvent for 10 min to 5 h respectively, then heat up for extraction 1-4 times, each time for 10 min to 5 h, to obtain an extract; (2) Combine the extracts and concentrate to a thick paste; (3) Dissolve an appropriate amount of donkey-hide gelatin in water and mix it with the above thick paste; Preferably, the method comprises the following steps: (1) Select the raw materials in one or several formulas, soak them in 5-30 times the amount of solvent for 15 min to 3 h respectively, then heat up for extraction 1-4 times, each time for 15 min to 3 h, to obtain an extract; (2) Combine the extracts and concentrate to a thick paste; (3) Dissolve an appropriate amount of donkey-hide gelatin in water and mix it with the above thick paste; Preferably, the method comprises the following steps: Collect rose aromatic water, and in step (3), dissolve an appropriate amount of donkey-hide gelatin in water and mix it with the above thick paste and rose aromatic water.

5. The preparation method of the composition according to claim 4, characterized in that, The extraction adopts water extraction and membrane separation method, decoction, reflux extraction, microwave extraction, ultrasonic extraction method, maceration method, dynamic extraction method, and / or vacuum extraction method; Preferably, the solvent is selected from: water, ethanol or methanol. Preferably, the temperature range for heating up is 45 to 100 °C, preferably 50 to 90 °C, preferably 55 to 85 °C, preferably 60 to 80 °C.

6. A preparation method for the manufactured product as claimed in claim 3, characterized in that, Comprising preparing by adding an acceptable excipient to the product in step (4) of claim 4 or 5; Preferably, the finished product is a drug, health food, food, external health product, additive or intermediate product; Preferably, the finished product is a paste-like food; Preferably, the finished product is used to increase glucose uptake, enhance ATPase activity, and increase cellular ATP content.

7. Use of the composition according to any one of claims 1-2 or the finished product according to claim 3 in the preparation of a product for preventing, treating or adjuvantly treating an increase in glucose uptake, enhancement of ATPase activity, and increase in cellular ATP content.

8. The application according to claim 7, characterized in that, The product can delay female aging.

9. The application according to claim 7 or 8, characterized in that, The product is a drug, health food, food, external health product, additive or intermediate product.

10. The application according to any one of claims 7 to 9, characterized in that The finished product is a paste-like food.

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