Natural raw material composition and product containing natural raw material composition and having anti-oxidation effect

Abstract

The invention relates to the technical field of antioxidant products, in particular to a natural raw material composition and a product which contains the natural raw material composition and has an antioxidant effect. The natural raw material composition is prepared from the following components in parts by weight: (20 to 450) parts of a feijoa sellowii extract, (5 to 150) parts of a pinus coralloides extract, (20 to 450) parts of a turmeric extract and (20 to 450) parts of ergothioneine. According to the present invention, the proper amounts of the feijoa sellowii extract, the pinus costus extract, the turmeric extract and the ergothioneine are matched to form the specific natural raw material formula, and the natural raw material formula has good oxidation resistance. In addition, the natural raw material formula can be used for preparing oral products, external products and the like, and a new choice is provided for development and application of the products.

Description

Technical Field

[0001] The present application relates to the technical field of antioxidant products, and particularly relates to a natural raw material composition and a product with antioxidant efficacy containing the natural raw material composition. Background Art

[0002] When oxygen is in a non-fully reduced state, reactive oxygen species (ROS) substances including superoxide anion, hydrogen peroxide, singlet oxygen, hydroxyl radical, etc. will be generated. Excessive accumulation of ROS can lead to oxidative damage to the body, and then cause cancers, cardiovascular diseases, neurodegenerative diseases, etc.; therefore, selecting appropriate types of antioxidants and expanding the application scope of current antioxidants has become a new research direction.

[0003] Antioxidation is an important issue in the field of great health and plays a crucial role in maintaining people's physical health. With the development of technology and the improvement of people's living standards, people are increasingly concerned about the antioxidant components and their effects in daily diets, and the demand for antioxidant components has stimulated the screening and research and development of a series of antioxidant components.

[0004] Antioxidant components are mainly extracted from natural materials (such as plants) and chemically synthesized. Compared with the chemical synthesis method, the antioxidant components extracted from natural plants have lower toxicity, and are rich in sources and easy to obtain. How to obtain natural raw materials with antioxidant efficacy is the technical problem to be solved by the present application. Summary of the Invention

[0005] Based on this, one or more embodiments of the present application provide a natural raw material composition and a product with antioxidant efficacy including the same. The following technical solutions are included:

[0006] One or more embodiments of the present application provide a natural raw material composition, and the natural raw material composition includes feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine in a weight ratio of (20 - 450) : (5 - 150) : (20 - 450) : (20 - 450).

[0007] In some embodiments of the present application, the natural raw material composition includes feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine in a weight ratio of (385 - 415) : (95 - 115) : (385 - 415) : (385 - 415).

[0008] In some embodiments of the present application, the natural raw material composition includes feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine in a weight ratio of (20 - 30) : (5 - 15) : (20 - 30) : (20 - 30).

[0009] One or more embodiments of the present application provide the application of the natural raw material composition in preparing a product with antioxidant efficacy.

[0010] One or more embodiments of the present application provide a product with antioxidant efficacy. The product includes the natural raw material composition and excipients. In the product, the concentrations of feijoa extract, maritime pine extract, turmeric extract and ergothioneine are 20 μg / mL - 450 μg / mL, 5 μg / mL - 150 μg / mL, 20 μg / mL - 450 μg / mL and 20 μg / mL - 450 μg / mL respectively.

[0011] In some embodiments of the present application, the concentrations of feijoa extract, maritime pine extract, turmeric extract and ergothioneine are 385 μg / mL - 415 μg / mL, 95 μg / mL - 115 μg / mL, 385 μg / mL - 415 μg / mL and 385 μg / mL - 415 μg / mL respectively.

[0012] In some embodiments of the present application, the concentrations of feijoa extract, maritime pine extract, turmeric extract and ergothioneine are 20 μg / mL - 30 μg / mL, 5 μg / mL - 15 μg / mL, 20 μg / mL - 30 μg / mL and 20 μg / mL - 30 μg / mL respectively.

[0013] In some embodiments of the present application, the excipients include a solvent.

[0014] In some embodiments of the present application, the solvent includes an inorganic solvent.

[0015] In some embodiments of the present application, the inorganic solvent includes water.

[0016] Compared with the prior art, the beneficial effects of the present application include:

[0017] The present application combines appropriate amounts of feijoa extract, maritime pine extract, turmeric extract and ergothioneine to form a specific natural raw material formula, which has good antioxidant performance. In addition, the natural raw material formula of the present application can be used to prepare oral products, topical products, etc., providing new options for the development and application of such products. Specific Embodiments

[0018] The present application will be further described in detail below in conjunction with embodiments and examples. It should be understood that these embodiments and examples are only used to illustrate the present application and not to limit the scope of the present application. The purpose of providing these embodiments and examples is to make the disclosure of the present application more thoroughly and comprehensively understood. It should also be understood that the present application can be implemented in many different forms and is not limited to the embodiments and examples described herein. Those skilled in the art can make various changes or modifications without departing from the connotation of the present application, and the equivalent forms obtained also fall within the protection scope of the present application. In addition, in the following description, a large number of specific details are given to provide a more thorough understanding of the present application. It should be understood that the present application can be implemented without one or more of these details.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs. The terms used in the specification of this application herein are only for the purpose of describing the embodiments and examples and are not intended to limit this application.

[0020] The term

[0021] Unless otherwise stated or there is a contradiction, the terms or phrases used herein have the following meanings:

[0022] The selection range of the terms “and / or”, “or / and”, “and / or” used herein includes any one of two or more related listed items, and also includes any and all combinations of the related listed items. The said any and all combinations include combinations of any two related listed items, any more related listed items, or all related listed items. It should be noted that when at least two conjunctions selected from “and / or”, “or / and”, “and / or” are used to connect at least three items, it should be understood that in this application, this technical solution undoubtedly includes the technical solution connected by “logical and”, and also undoubtedly includes the technical solution connected by “logical or”. For example, “A and / or B” includes three parallel solutions: A, B, and A + B. Another example, the technical solution of “A, and / or, B, and / or, C, and / or, D” includes any one of A, B, C, D (that is, the technical solution connected by “logical or”), and also includes any and all combinations of A, B, C, D, that is, includes combinations of any two or any three of A, B, C, D, and also includes the combination of the four items A, B, C, D (that is, the technical solution connected by “logical and”).

[0023] In this application, the terms “multiple”, “diverse”, “multiple times”, “multiple elements”, etc., unless otherwise specified, refer to a quantity greater than 2 or equal to 2. For example, “one or more” means one or greater than or equal to two.

[0024] As used herein, "its combination", "any combination thereof", "any combination mode thereof", etc. include all suitable combination modes of any two or more of the listed items.

[0025] In this application, the "suitable" in "suitable combination mode", "suitable mode", "any suitable mode", etc. is subject to being able to implement the technical solution of this application, solve the technical problems of this application, and achieve the expected technical effects of this application.

[0026] In this application, "preferred", "better", "more preferable", "it is advisable" are only used to describe the implementation modes or embodiments with better effects, and it should be understood that they do not constitute a limitation on the protection scope of this application.

[0027] In this application, "further", "even further", "especially", etc. are used for descriptive purposes and indicate differences in content, but should not be understood as a limitation on the protection scope of this application.

[0028] In this application, "optionally", "optional", "option" mean that it is optional, that is, it refers to any one of the two parallel options of "yes" or "no". If "optional" appears multiple times in a technical solution, without special instructions and without contradictions or mutual restrictions, each "optional" is independent.

[0029] In this application, in "the first aspect", "the second aspect", "the third aspect", "the fourth aspect", etc., the terms "first", "second", "third", "fourth", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or quantity, nor can it be understood as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "first", "second", "third", "fourth", etc. only serve the purpose of non-exhaustive enumeration and description, and it should be understood that they do not constitute a closed limitation on quantity.

[0030] In this application, for the technical features described in an open-ended manner, it includes the closed technical solutions composed of the listed features, and also includes the open-ended technical solutions containing the listed features.

[0031] In this application, when it comes to numerical intervals (i.e., numerical ranges), unless otherwise specified, the selectable numerical values are considered continuous within the above-mentioned numerical intervals, and include the two numerical endpoints of the numerical range (i.e., the minimum value and the maximum value), as well as each numerical value between these two numerical endpoints. Unless otherwise specified, when the numerical interval only refers to the integers within the numerical interval, it includes the two endpoint integers of the numerical range, as well as each integer between the two endpoints. In this article, it is equivalent to directly listing each integer. For example, if t is an integer selected from 1 to 10, it means that t is any integer selected from the integer group consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10. In addition, when multiple ranges are provided to describe features or characteristics, these ranges can be combined. In other words, unless otherwise specified, the ranges disclosed in this article should be understood to include any and all sub-ranges subsumed therein.

[0032] The temperature parameter in this application, unless otherwise specified, allows both constant temperature treatment and variation within a certain temperature range. It should be understood that the so-called constant temperature treatment allows the temperature to fluctuate within the accuracy range controlled by the instrument. Fluctuations within ranges such as ±5°C, ±4°C, ±3°C, ±2°C, and ±1°C are allowed.

[0033] In this application, %(w / w) and wt% both represent weight percentages, %(v / v) refers to volume percentages, and %(w / v) refers to mass-volume percentages.

[0034] All documents mentioned in this application are cited in this application as references, just as if each document was cited separately as a reference. Unless it conflicts with the application purpose and / or technical solution of this application, the cited documents related to this application are cited for all their contents and all their purposes. When this application involves cited documents, the definitions of relevant technical features, terms, nouns, phrases, etc. in the cited documents are also cited. When this application involves cited documents, the examples and preferred methods of the relevant technical features cited can also be incorporated into this application as references, but only to the extent that this application can be implemented. It should be understood that when the cited content conflicts with the description in this application, this application shall prevail or be amended adaptively according to the description in this application.

[0035] In the first aspect of the embodiments of the present application, a natural raw material composition is provided. The natural raw material composition includes feijoa extract, maritime pine extract, turmeric extract, and ergothioneine in a weight ratio of (20 - 450):(5 - 150):(20 - 450):(20 - 450). In this natural raw material composition, the weight parts of the feijoa extract are, for example, 20, 50, 100, 150, 200, 250, 300, 350, 400, 450; the weight parts of the maritime pine extract are, for example, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150; the weight parts of the turmeric extract are, for example, 20, 50, 100, 150, 200, 250, 300, 350, 400, 450; and the weight parts of the ergothioneine are, for example, 20, 50, 100, 150, 200, 250, 300, 350, 400, 450.

[0036] The proportions of the components in the natural raw material composition defined in the present application are their working proportions, that is, the weight ratios during use. On this basis, the present application does not make special limitations on the natural raw material composition, which can be of the ready-to-use type or the ready-to-prepare type.

[0037] In some embodiments of the present application, the natural raw material composition includes feijoa extract, maritime pine extract, turmeric extract, and ergothioneine in a weight ratio of (385 - 415):(95 - 115):(385 - 415):(385 - 415). In this natural raw material composition, the weight parts of the feijoa extract are, for example, 385, 390, 395, 400, 405, 410, 415; the weight parts of the maritime pine extract are, for example, 95, 100, 105, 110, 115; the weight parts of the turmeric extract are, for example, 385, 390, 395, 400, 405, 410, 415; and the weight parts of the ergothioneine are, for example, 385, 390, 395, 400, 405, 410, 415.

[0038] In some embodiments of the present application, the natural raw material composition comprises feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine in a weight ratio of (20-30):(5-15):(20-30):(20-30). In this natural raw material composition, the weight parts of feijoa extract are, for example, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30; the weight parts of pinus pinaster extract are, for example, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15; the weight parts of turmeric extract are, for example, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30; and the weight parts of ergothioneine are, for example, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30.

[0039] In the second aspect of the embodiments of the present application, there is provided an application of the described natural raw material composition in the preparation of a product with antioxidant efficacy. The present application does not make any particular limitation on the types of products with antioxidant efficacy, including but not limited to foods, medicines, and skin care products, which can be used externally or orally.

[0040] In the third aspect of the embodiments of the present application, there is provided a product with antioxidant efficacy, the product comprising the described natural raw material composition and excipients. In the product, the concentrations of the feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine are 20 μg / mL - 450 μg / mL (for example, 20, 35, 50, 75, 100, 125, 150, 175, 200, 225, 250, 225, 300, 325, 350, 375, 400, 425, 450 μg / mL), 5 μg / mL - 150 μg / mL (for example, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 115, 120, 125, 130, 135, 140, 145, 150 μg / mL), 20 μg / mL - 450 μg / mL (for example, 20, 35, 50, 75, 100, 125, 150, 175, 200, 225, 250, 225, 300, 325, 350, 375, 400, 425, 450 μg / mL) and 20 μg / mL - 450 μg / mL (for example, 20, 35, 50, 75, 100, 125, 150, 175, 200, 225, 250, 225, 300, 325, 350, 375, 400, 425, 450 μg / mL), respectively.

[0041] In some embodiments of the present application, the concentrations of feijoa extract, pinus pinaster extract, curcuma longa extract, and ergothioneine are 385 μg / mL - 415 μg / mL (for example, 385, 390, 395, 400, 405, 410, 415 μg / mL), 95 μg / mL - 115 μg / mL (for example, 95, 100, 105, 110, 115 μg / mL), 385 μg / mL - 415 μg / mL (for example, 385, 390, 395, 400, 405, 410, 415 μg / mL), and 385 μg / mL - 415 μg / mL (for example, 385, 390, 395, 400, 405, 410, 415 μg / mL), respectively.

[0042] In some embodiments of the present application, the concentrations of feijoa extract, pinus pinaster extract, curcuma longa extract, and ergothioneine are 20 μg / mL - 30 μg / mL (for example, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 μg / mL), 5 μg / mL - 15 μg / mL (for example, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 μg / mL), 20 μg / mL - 30 μg / mL (for example, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 μg / mL), and 20 μg / mL - 30 μg / mL (for example, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 μg / mL), respectively.

[0043] The present application does not particularly limit the types of products with antioxidant effects, including but not limited to foods, drugs, and skin care products. It can be understood that the products of the present application may include excipients, such as food excipients, pharmaceutically acceptable excipients, and cosmetic excipients. The excipients include but are not limited to solvents. In some embodiments of the present application, the solvent includes an inorganic solvent, such as water.

[0044] The embodiments of the present application will be described in detail below in conjunction with examples. It should be understood that these examples are only used to illustrate the present application and not to limit the scope of the present application. For the experimental methods without specific conditions in the following examples, the guidance given in the present application is preferably referred to, and it can also be carried out according to the experimental manuals or conventional conditions in the art, or according to the conditions recommended by the manufacturer, or referring to the experimental methods known in the art.

[0045] In the following specific examples, for the measurement parameters of raw material components, if there is no special description, there may be slight deviations within the weighing accuracy range. For temperature and time parameters, acceptable deviations caused by instrument test accuracy or operation accuracy are allowed.

[0046] Example 1

[0047] I. Experimental Reagents

[0048] 1. DPPH (1,1-diphenyl-2-picrylhydrazyl; HY-112053) was purchased from MedChemExpress, USA.

[0049] 2. Trolox (water-soluble vitamin E, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; HY-101445) was purchased from MedChemExpress, USA.

[0050] 3. Absolute ethanol was purchased from Fuchen (Tianjin) Chemical Reagent Co., Ltd., China.

[0051] 4. Feijoa extract was purchased from Shanghai Huabao Peacock Flavor Co., Ltd.

[0052] 5. Maritime pine extract was purchased from Shaanxi Jiahe Biotechnology Co., Ltd.

[0053] 6. Turmeric extract was purchased from Shanghai Huabao Peacock Flavor Co., Ltd.

[0054] 7. Ergothioneine was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0055] II. Experimental Methods

[0056] 1. In vitro DPPH free radical scavenging assay

[0057] 1.1 Reagent Preparation

[0058] (1) Preparation of working solution

[0059] Weigh 0.1 mg of Trolox powder and dissolve it in 1 mL of absolute ethanol. After complete dissolution, a standard solution with a concentration of 100 μg / mL is prepared.

[0060] Weigh 7.89 mg of DPPH powder and dissolve it in 100 mL of absolute ethanol. After mixing evenly, the detection working solution is obtained.

[0061] (2) Sample dilution

[0062] Prepare each experimental sample with double-distilled water, feijoa extract, maritime pine extract, turmeric extract, and ergothioneine. The formula of the experimental samples is shown in Table 2.

[0063] 1.2 Experimental operation

[0064] 1.2.1 Preparation of standard curve

[0065] The Trolox standard solution was serially diluted with absolute ethanol to obtain solutions with gradient concentrations of 100, 80, 60, 40, 20, and 0 μg / mL in sequence.

[0066] 20 μL of the standard solution or test experimental sample at the above concentrations and 80 μL of absolute ethanol were successively added to a 96-well plate, denoted as "experimental wells".

[0067] In addition, 100 μL and 200 μL of absolute ethanol were added to two other wells in the 96-well plate. The former was denoted as the "control well", and the latter was denoted as the "background well".

[0068] The DPPH detection working solution was poured into the sample addition groove. Using a multi-channel pipette, 100 μL of the DPPH detection working solution was added to the experimental wells and the control well. After incubating in the dark at room temperature for 30 minutes, the absorbance was measured at 517 nm using a microplate reader.

[0069] The formula for calculating the free radical scavenging rate is: Free radical scavenging rate (%) = { (OD 对照孔 - OD 背景孔 ) - (OD 实验孔 - OD 背景孔 )} / (OD 对照孔 - OD 背景孔 ). The added volume and components of each well are shown in Table 1:

[0070] Table 1. Added volume and components of each well

[0071] Experimental well Control well Background well Standard / sample to be measured (μL) 20 / / Absolute ethanol (μL) 80 100 200 DPPH working solution (μL) 100 100 /

[0072] The results of the DPPH free radical scavenging rate are shown in the following table:

[0073] Table 2

[0074]

[0075] The results showed that:

[0076] 1 - In experimental sample 1, it contained feijoa extract (400 μg / mL), maritime pine extract (100 μg / mL), turmeric extract (400 μg / mL), and ergothioneine (400 μg / mL) simultaneously. Compared with experimental sample 6 (containing only 400 μg / mL feijoa extract), experimental sample 10 (containing only 100 μg / mL maritime pine extract), experimental sample 13 (containing only 400 μg / mL turmeric extract), and experimental sample 15 (containing only 400 μg / mL ergothioneine), the DPPH free radical scavenging rate was effectively improved.

[0077] Similarly, the DPPH radical scavenging rates of 1-Experimental Sample 2 and 1-Experimental Sample 3 were also effectively improved compared with the single raw material experimental samples with corresponding concentrations.

[0078] In particular, for 1-Experimental Sample 3, its DPPH radical scavenging rate was higher than the sum of the DPPH radical scavenging rates of 1-Experimental Sample 8, 1-Experimental Sample 12, 1-Experimental Sample 14, and 1-Experimental Sample 16, indicating that the raw materials in 1-Experimental Sample 3 exerted a synergistic effect.

[0079] 2. ACE Inhibition Rate Experiment

[0080] Angiotensin-converting enzyme (ACE) is a dipeptidyl carboxylase that plays an important role in the regulation of human blood pressure and is mainly involved in the regulation of the renin-angiotensin-aldosterone system. ACE catalyzes the hydrolysis of angiotensin I into angiotensin II with strong blood pressure-raising effects, and at the same time decomposes the vasodilatory bradykinin into inactivated fragments, leading to an increase in blood pressure. Therefore, inhibiting the activity of ACE reduces the production of angiotensin II and the inactivation of bradykinin, thereby achieving the purpose of lowering blood pressure.

[0081] 2.1 Reagents

[0082] (1) Angiotensin-converting enzyme (ACE): SAE0075, Sigma, USA.

[0083] (2) Hippuryl-His-Leu (HHL): 859052, Sigma, USA.

[0084] (3) Prepare each experimental sample according to the description under "1. In Vitro DPPH Radical Scavenging Detection Experiment".

[0085] 2.2 Methods

[0086] Dissolve each experimental sample and the HHL substrate in 0.1 M sodium borate buffer (pH 8.3) containing 0.3 M NaCl. Take 25 μL of the 1 mg / mL sample solution and mix it evenly with 50 μL of the 4.7 mM Hip-His-Leu solution (HHL solution), and incubate it in a 37°C water bath for 5 min. Add 50 μL of the 0.025 U / mL ACE enzyme and react in a 37°C water bath for 30 min. Immediately add 0.75 mL of 0.3 M NaOH to terminate the reaction after the reaction ends. Add 50 μL of 2% (w / v) o-phthalaldehyde to the mixed solution, and after reacting in the dark for 10 min, add 100 μL of 3 M HCl to end the reaction. Measure the fluorescence of the reaction solution with an enzyme-labeled instrument at an excitation wavelength of 340 nm and an emission wavelength of 455 nm.

[0087] The calculation formula for ACE inhibition rate is as follows:

[0088] ACE inhibition rate (%) = [1 - (F sample - F blank control) / F negative control] × 100%;

[0089] F sample: 25 μL sample solution + 50 μL HHL solution + 50 μL ACE enzyme;

[0090] F blank control: 25 μL sodium borate buffer + 50 μL HHL solution + 50 μL sodium borate buffer;

[0091] F negative control: 25 μL sodium borate buffer + 50 μL HHL solution + 50 μL ACE enzyme.

[0092] Table 3

[0093]

[0094] The technical features of the above-described embodiments and examples can be combined in any suitable manner. For the sake of brevity of description, not all possible combinations of the technical features in the above-described embodiments and examples are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered to be within the scope described in this specification.

[0095] The above-described examples only represent several embodiments of the present application, which are convenient for understanding the technical solutions of the present application specifically and in detail, but should not be construed as a limitation on the scope of patent protection of the application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. In addition, it should be understood that after reading the above teachings of the present application, those skilled in the art can make various changes or modifications to the present application, and the equivalent forms obtained also fall within the protection scope of the present application. It should also be understood that the technical solutions obtained by those skilled in the art through logical analysis, reasoning or limited experiments based on the technical solutions provided in the present application are all within the protection scope of the appended claims of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the content of the appended claims, and the specification can be used to explain the content of the claims.

Claims

1. A natural raw material composition, characterized in that, The natural raw material composition includes feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine with a weight ratio of (20 - 450):(5 - 150):(20 - 450):(20 - 450).

2. The natural raw material composition according to claim 1, characterized in that, The natural raw material composition includes feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine with a weight ratio of (385 - 415):(95 - 115):(385 - 415):(385 - 415).

3. The natural raw material composition according to claim 1, characterized in that, The natural raw material composition includes feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine with a weight ratio of (20 - 30):(5 - 15):(20 - 30):(20 - 30).

4. Use of the natural raw material composition according to any one of claims 1 to 3 in the preparation of a product having antioxidant efficacy.

5. A product with antioxidant efficacy, characterized in that, The product includes the natural raw material composition according to claim 1 and excipients. In the product, the concentrations of the feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine are 20 μg / mL - 450 μg / mL, 5 μg / mL - 150 μg / mL, 20 μg / mL - 450 μg / mL and 20 μg / mL - 450 μg / mL respectively.

6. The product with antioxidant efficacy according to claim 5, characterized in that, The concentrations of the feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine are 385 μg / mL - 415 μg / mL, 95 μg / mL - 115 μg / mL, 385 μg / mL - 415 μg / mL and 385 μg / mL - 415 μg / mL respectively.

7. The product with antioxidant efficacy according to claim 5, characterized in that, The concentrations of the feijoa extract, pinus pinaster extract, turmeric extract and ergothioneine are 20 μg / mL - 30 μg / mL, 5 μg / mL - 15 μg / mL, 20 μg / mL - 30 μg / mL and 20 μg / mL - 30 μg / mL respectively.

8. The product with antioxidant efficacy according to any one of claims 5 to 7, characterized in that The excipients include a solvent.

9. The product with antioxidant efficacy according to claim 8, characterized in that, The solvent includes an inorganic solvent.

10. The product with antioxidant efficacy according to claim 9, characterized in that, The inorganic solvent includes water.

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