Sunscreen repairing composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of sunscreen products, in particular to a sunscreen repairing composition as well as a preparation method and application thereof. The sunscreen repairing composition comprises a skin conditioner, a sunscreen agent and auxiliary materials, the skin conditioning agent comprises a sunflower bud extract (Helianthus Annuus (Sunflower) Sputum Extract), and the skin conditioning agent is prepared from the Helianthus Annuus (Sunflower) Sputum Extract. The composition provides a function of protecting mitochondria, especially can reduce the influence of sunlight on mitochondrial membrane potential and maintain the stability of the mitochondrial membrane potential, thereby reducing cell death. The composition has anti-inflammatory, repairing and sunscreen effects, contains the sunflower bud extract, not only can effectively protect mitochondria in skin epidermis cells, but also can enable the mitochondria to be healthier and generate more ATP under UV irradiation, so that the skin cells have enough energy to ensure normal running of life activities. Meanwhile, inflammation can be inhibited, the skin redness phenomenon is reduced, the long-acting skin protection effects such as repairing and sun blocking are achieved, and the skin problem can be solved in an all-around mode.

Description

Technical Field

[0001] The present invention relates to the technical field of sunscreen products, and particularly to a sunscreen repair composition, a preparation method thereof and an application thereof. Background Art

[0002] Research shows that about 80% of skin aging is caused by solar ultraviolet rays. Therefore, a sunscreen product with complete functions has become a necessity in summer. Among existing products, there are also whitening, moisturizing, repair and sunscreen products. However, even if sunscreen is used, consumers still find that skin damage will still occur when the exposure time is relatively long. If the sunscreen effect is excluded, will a similar result still occur when using an equivalent sunscreen? Will ultraviolet rays still damage the skin, even leading to the death of skin cells, the appearance of skin wrinkles or other skin aging problems?

[0003] As is well known, mitochondria are an important organelle in cells, the "energy factory" in cells, responsible for generating ATP to provide energy for various activities of cells; in addition to energy supply, it also participates in all aspects of various cell life activities, including respiration for basic cell survival, metabolism for regulating cell life processes, apoptosis at the end of cell life and signal transduction in the process of cell life activities. At the same time, it is found that mitochondrial membrane potential is an important index for evaluating mitochondrial function, and its stability is crucial for maintaining normal cell physiological functions. The decrease of mitochondrial membrane potential is also the earliest manifestation of cell apoptosis. Mitochondrial membrane potential is the electrochemical gradient across the inner mitochondrial membrane, mainly generated by the electron transport chain, which is crucial for ATP synthesis. It is the prerequisite for maintaining mitochondrial oxidative phosphorylation and generating ATP, and is also necessary for maintaining mitochondrial function. Therefore, mitochondrial membrane potential can directly reflect the functional state of mitochondria: when the mitochondrial membrane potential decreases, it often means that mitochondrial function is damaged. Therefore, the change of mitochondrial membrane potential is closely related to the occurrence and development of various diseases. For example, in diseases such as cardiovascular diseases, tumors and metabolic disorders, mitochondrial function disorders and abnormal membrane potentials often occur.

[0004] When ultraviolet radiation damages skin cells, it will lead to an increase in reactive oxygen species (ROS), which in turn affects mitochondrial function, reduces mitochondrial membrane potential (MMP), results in a decrease in ATP synthesis, and ultimately triggers cell apoptosis or damage. So far, there is no sunscreen product on the market that can reduce the damage of ultraviolet rays to cell mitochondria and maintain the stability of the mitochondrial membrane potential of skin cells.

[0005] As science continues to progress and consumers are also growing, the requirements for the safety and efficacy of sunscreen products are getting higher and higher. Zinc oxide has attracted much attention as a safe and effective ultraviolet filter. It is a broad-spectrum ultraviolet filtering component that can protect the skin from both UVB and UVA damage simultaneously. In particular, it has a relatively long UVA wavelength coverage range, which is difficult for many sunscreen ingredients to achieve. Unfortunately, zinc oxide forms divalent zinc ions when the pH < 7 and is incompatible with ionic rheology modifiers such as carbomer. Even after surface treatment, it is difficult for zinc oxide to coexist stably with carbomer for a long time. In the production of cosmetics, acrylic derivative polymer products such as carbomer are the most widely used thickeners and are also ionic-sensitive rheology modifiers. Such components are contained in 80% of the sunscreen products on the market. Therefore, how to make these two raw materials coexist stably in the formula is also a problem worthy of discussion.

[0006] Finally, when making products containing a large amount of powders such as titanium dioxide or zinc oxide, R & D personnel often use dispersants to help disperse them. Most of the existing dispersants are synthetic components, such as polyhydroxystearic acid. However, in the natural environment, the degradation rate of polyhydroxystearic acid is relatively slow, and it may accumulate in the environment, causing potential impacts on the environment, especially in the case of large-scale use. Can some natural plant-derived components be used to replace it and have comparable or better dispersion effects? The above problems urgently need to be solved by R & D personnel. Summary of the Invention

[0007] The main object of the present invention is to provide a sunscreen repair composition, a preparation method and its application, aiming to solve the technical problem that existing sunscreen products cannot maintain the stability of the mitochondrial membrane potential of skin cells.

[0008] To achieve the above object, the present invention provides a sunscreen repair composition, comprising a skin conditioner, a sunscreen agent and excipients; the skin conditioner includes sunflower bud extract.

[0009] Preferably, the excipients include at least one of a humectant, a chelating agent, a thickening agent, a neutralizing agent, an emollient, a skin feel modifier, an emulsifier, a preservative, a humectant, a skin conditioner and a solvent.

[0010] Preferably, calculated by the total weight percentage of the sunscreen repair composition, the sunscreen repair composition comprises the following components:

[0011] Phase A components: the first humectant 1-10%, chelating agent 0.05-0.1%, neutralizing agent 0.01-0.1%, the first thickening agent 0.05-0.15% and solvent 1-50%;

[0012] Phase B components: emollient 1-25%, the sunscreen agent 0.2-66%, skin feel regulator 0.1-10%, emulsifier 0.15-8% and second thickener 0.05-2%; the sunscreen agent includes zinc oxide;

[0013] Phase C components: third thickener 0.05-2%, preservative 0.8-2.3% and second humectant 0.05-1.5%;

[0014] Phase D components: the skin conditioner 1-5%.

[0015] Preferably, the sunscreen agent in the Phase B components further includes at least one of octyltriethoxysilane, titanium dioxide, ethylhexyl methoxycinnamate, ethylhexyl salicylate, avobenzone, phenylbenzimidazole sulfonic acid, diethylamino hydroxybenzoyl hexyl benzoate, terephthalylidene dicamphor sulfonic acid, ethylhexyl salicylate, homosalate, bis-ethylhexyloxyphenol methoxyphenyl triazine, octocrylene, phenylbenzimidazole sulfonic acid, benzophenone-3, benzotriazolyl dodecyl p-cresol, ethylhexyl triazone, methylene bis-benzotriazolyl tetramethylbutylphenol and polysiloxane-15;

[0016] The emollient in the Phase B components further includes at least one of cyclomethicone, isononyl isononanoate, trimethylpentaphenyl trisiloxane, diphenylsilanoxy phenyl polytrimethylsiloxane, isocetane, cyclomethicone, C12-15 alkyl benzoate, octyldodecanol, lauryl laurate, CRAMBE ABYSSINICA seed oil, phytosterols, shea butter, phytosqualane, jojoba oil, sunflower seed oil, limnanthes alba seed oil, grape seed oil, macadamia nut oil, olive oil, palm oil, cocoa butter, jojoba butter, glycolipids, octyldodecanol, caprylic / capric triglyceride and phytosterol;

[0017] The skin feel regulator in the Phase B components includes at least one of silica, behenyl alcohol, polymethylsilsesquioxane, dimethicone / vinyl dimethicone crosspolymer, methyl methacrylate crosspolymer, lauroyl lysine and distearyldimethylammonium chloride;

[0018] The emulsifier in the Phase B components includes at least one of polyglyceryl-3 polyricinoleate, polysorbate 80, cetearyl alcohol, cocoyl glucoside, glyceryl stearate, polyglyceryl-3 diisostearate, glycerol-2 triisostearate, polyglyceryl 2-dipolyhydroxystearate, bis(lauroylglutamyl)lysine sodium, polysorbate 60, polyglyceryl-10 stearate and PEG-100 stearate;

[0019] The second thickener in the Phase B components includes stearic acid.

[0020] Preferably, the first humectant in the A-phase component includes at least one of butanediol, propylene glycol, and glycerol;

[0021] The chelating agent in the A-phase component includes disodium EDTA; the neutralizing agent in the A-phase component includes at least one of potassium hydroxide, sodium hydroxide, or TEA; the first thickening agent in the A-phase component includes carbomer or xanthan gum; the solvent in the A-phase component includes water.

[0022] Preferably, the third thickening agent in the C-phase component includes at least one of sodium acrylate / sodium acryloyldimethyl taurate copolymer and polyacrylamide;

[0023] The preservative in the C-phase component includes at least one of phenoxyethanol, triethylene glycol, and ethylhexylglycerin;

[0024] The second humectant in the C-phase component includes at least one of 1,2-hexanediol and caprylyl glycol.

[0025] Preferably, the skin conditioner further includes at least one of Caesalpinia spinosa fruit extract, propylene glycol, phenoxyethanol, sodium benzoate, and water.

[0026] In addition, the present invention also provides a preparation method of a sunscreen repair composition, including the following steps:

[0027] S1. According to the ratio, mix the raw materials of the A-phase component and stir evenly to obtain the A-phase component;

[0028] S2. First, mix the sunscreen agent - zinc oxide in the B-phase component with an acid solution, then treat it with sodium hydroxide or potassium hydroxide with a concentration of 0.2 - 5%, and finally calcine it at 100 - 400°C for 1 - 10 h, grind it, control the contact points between the surface of zinc oxide and triethoxysilane octane, and ensure that the final particle size of zinc oxide particles is 15 - 35 nm; then add deep-sea two-joint watercress oil for dispersion, and then homogenize and disperse the dispersed zinc oxide, the remaining sunscreen agents, and the remaining raw materials in the B-phase component to obtain the B-phase component;

[0029] S3. Heat the A-phase component and the B-phase component to 65 - 80°C respectively, mix them, homogenize them, then add the raw materials of the C-phase component for homogenization, stir and cool down to 40 - 50°C, and then add the raw materials of the D-phase component, stir evenly, and then obtain the sunscreen repair composition.

[0030] In addition, the present invention also provides an application of the above sunscreen repair composition, and the sunscreen repair composition is used to maintain the stability of mitochondrial membrane potential.

[0031] Compared with the prior art, the sunscreen repair composition and preparation method of the present invention have the following beneficial effects:

[0032] 1. This solution provides a composition that protects mitochondrial function and has anti-inflammatory, repair, and sunscreen effects. The composition contains sunflower sprout extract, which can reduce the impact of sunlight on the mitochondrial membrane potential, maintain the stability of the mitochondrial membrane potential (MMP), completely eliminate the damage of UV to cells, and thus reduce cell death. This composition can effectively protect the mitochondria in skin epidermal cells, making the production of ATP exceed the original level, making the cells more energetic and producing more energy, so that skin cells have enough energy to ensure the normal operation of life activities. At the same time, this composition can inhibit the inflammatory factor IL-1β, completely inhibit the inflammatory factors caused by UV to the normal level, reduce the skin redness phenomenon, and has long-term skin protection effects such as repair and sunscreen, and can comprehensively improve skin problems.

[0033] 2. In the preparation method, zinc oxide is first acidified, then calcined and ground at high temperature, the contact points between the surface of zinc oxide and triethoxysilane are controlled, and the final zinc oxide particles reach 15 - 35 nanometers, so that zinc oxide and carbomer can coexist stably for a long time.

[0034] 3. Finally, deep-sea cocheti oil (active substance) is used as the dispersant of zinc oxide, which has a better dispersion effect, can form a continuous and dense protective film, reduce the ultraviolet penetration holes caused by local aggregation, and the sunscreen effect is more stable. Description of the Drawings

[0035] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other relevant drawings can also be obtained based on these drawings.

[0036] Figure 1 It shows the decline of mitochondrial membrane potential after UV light irradiation of different experimental groups of this solution;

[0037] Figure 2 It shows the ATP production after UV light irradiation of different experimental groups of this solution;

[0038] Figure 3 It shows the data of reactive oxygen fluorescence intensity after UV light irradiation of different experimental groups of this solution;

[0039] Figure 4 It shows the data of IL-1β concentration after UV light irradiation of different experimental groups of this solution;

[0040] Figure 5 Absorbance data of different experimental groups of this solution at different wavelengths;

[0041] Figure 6 Scanning electron micrographs of Example 2 and Comparative Example 1 of this solution after dispersion.

[0042] The realization, functional features and advantages of the purpose of this application will be further described in conjunction with the embodiments and with reference to the accompanying drawings. Detailed implementation manners

[0043] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0044] In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present invention.

[0045] A sunscreen repair composition, calculated by the total weight percentage of the sunscreen repair composition, the sunscreen repair composition comprises the following components:

[0046] Phase A components: the first humectant 1-10%, chelating agent 0.05-0.1%, neutralizing agent 0.01-0.1%, the first thickener 0.05-0.15% and solvent 1-50%; wherein, the first humectant includes at least one of butylene glycol, propylene glycol and glycerol; the chelating agent includes disodium EDTA; the neutralizing agent includes at least one of potassium hydroxide, sodium hydroxide or TEA (triethylamine); the first thickener includes carbomer or xanthan gum; the solvent includes water;

[0047] Phase B components: emollient 1 - 25%, the sunscreen 0.2 - 66%, skin feel regulator 0.1 - 10%, emulsifier 0.15 - 8% and second thickener 0.05 - 2%; the sunscreen includes zinc oxide; it also includes at least one of n - octyltriethoxysilane, titanium dioxide, ethylhexyl methoxycinnamate, ethylhexyl salicylate, avobenzone, phenylbenzimidazole sulfonic acid, diethylamino hydroxybenzoyl hexyl benzoate, terephthalylidene dicamphor sulfonic acid, ethylhexyl salicylate, homosalate, bis - ethylhexyloxyphenol methoxyphenyl triazine, octocrylene, phenylbenzimidazole sulfonic acid, benzophenone - 3, benzotriazolyl dodecyl p - cresol, ethylhexyl triazone, methylene bis - benzotriazolyl tetramethylbutylphenol and polysiloxane - 15; the emollient also includes at least one of cyclodimethicone, isononyl isononanoate, trimethylpentaphenyl trisiloxane, diphenylsilanoxy phenyl polytrimethylsiloxane, isocetane, cyclodimethicone, C12 - 15 alkyl benzoate, octyldodecanol, lauryl laurate, CRAMBE ABYSSINICA seed oil, phytosterols, shea butter, phytosqualane, jojoba oil, sunflower seed oil, Limnanthes alba (MEYER) BRUGGEMAN seed oil, grape seed oil, macadamia nut oil, olive oil, palm oil, cocoa butter, jojoba butter, glycolipids, octyldodecanol, caprylic / capric triglyceride and phytosterols; the skin feel regulator includes at least one of silica, behenyl alcohol, polymethylsilsesquioxane, dimethicone / vinyl dimethicone crosspolymer, methyl methacrylate crosspolymer, lauroyl lysine and distearyldimethylammonium chloride; the emulsifier includes at least one of polyglyceryl - 3 polyricinoleate, polysorbate - 80, cetearyl alcohol, cocoyl glucoside, glyceryl stearate, polyglyceryl - 3 diisostearate, glyceryl - 2 triisostearate, polyglyceryl 2 - dimer dilinoleate, bis (laurylamido glutamine) lysine sodium, polysorbate 60, polyglyceryl - 10 stearate and PEG - 100 stearate; the second thickener includes stearic acid;

[0048] Phase C components: third thickener 0.05 - 2%, preservative 0.8 - 2.3% and second humectant 0.05 - 1.5%; the third thickener includes at least one of sodium acrylate / sodium acryloyldimethyltaurate copolymer, polyacrylamide; the preservative includes at least one of phenoxyethanol, triethylene glycol and ethylhexylglycerin; the second humectant includes at least one of 1,2 - hexanediol and caprylyl glycol;

[0049] Phase D components: the skin conditioner 1 - 5%; the skin conditioner includes sunflower sprout extract, and the skin conditioner also includes at least one of Caesalpinia spinosa fruit extract, propylene glycol, phenoxyethanol, sodium benzoate and water.

[0050] The present invention provides a composition that protects mitochondrial function and has anti-inflammatory, repair, and sunscreen effects. The composition contains sunflower sprout extract. This composition can reduce the impact of sunlight on mitochondrial membrane potential, maintain the stability of mitochondrial membrane potential (MMP), completely eliminate the damage of UV to cells, and thus reduce cell death. The decline of mitochondrial membrane potential is a sign of apoptosis. Therefore, protecting the impact on mitochondrial membrane potential in cells plays a crucial role in protecting cells and even the entire skin against sunlight damage and repair. This composition can also effectively protect the mitochondria in skin epidermal cells, making the production of its ATP exceed the original level, making the cells more energetic and producing more energy, so that skin cells have enough energy to ensure the normal operation of life activities. At the same time, this composition can inhibit the inflammatory factor IL-1β, completely inhibit the inflammatory factors caused by UV to the normal level, reduce the skin redness phenomenon, and has long-term skin protection effects such as repair and sunscreen, and can comprehensively improve skin problems.

[0051] In the components of the sunscreen repair composition, a sunscreen agent and various excipients are also added. The prepared sunscreen repair composition can block ultraviolet rays, has a good sunscreen effect, and at the same time, can also play roles in antioxidant, soothing, and repairing the barrier, enhancing the skin care function other than sunscreen.

[0052] The present invention also provides a preparation method of a sunscreen repair composition, which includes the following steps:

[0053] S1. According to the ratio, mix the raw materials of the A-phase components and stir evenly to obtain the A-phase components;

[0054] S2. First, mix the sunscreen agent - zinc oxide in the B-phase components with at least one of hydrochloric acid solution, sulfuric acid solution, or nitric acid solution, then treat it with sodium hydroxide or potassium hydroxide with a concentration of 0.2 - 5%, and finally calcine it at 100 - 400 °C for 1 - 10 h, grind it, control the contact points between the surface of zinc oxide and triethoxyoctylsilane, and ensure that the final particle size of zinc oxide particles is 15 - 35 nm; then add deep-sea two-jointed cress oil for dispersion, and then homogenize and disperse the dispersed zinc oxide, the remaining sunscreen agent, and the remaining raw materials in the B-phase components to obtain the B-phase components;

[0055] S3. Heat the A-phase components and the B-phase components to 65 - 80 °C respectively, then mix them, homogenize them, then add the raw materials of the C-phase components for homogenization, stir and cool down to 40 - 50 °C, and then add the raw materials of the D-phase components and stir evenly to obtain the sunscreen repair composition.

[0056] The sunscreen repair composition prepared by the above raw materials and preparation method can be used to maintain the stability of mitochondrial membrane potential.

[0057] Currently, polyhydroxystearic acid is usually used as a dispersant for zinc oxide in existing sunscreen compositions. It is found in this solution that when using Crambe abyssinica seed oil (active ingredient) as a dispersant, it has a better dispersion effect, can form a continuous and dense protective film, reduce the ultraviolet penetration holes caused by local aggregation, and the sunscreen effect is more stable.

[0058] In order to further optimize the production and treatment process of zinc oxide and avoid the problem that zinc oxide is difficult to exist stably with carbomer for a long time, in this solution, zinc oxide is acidified by compounding one or several of hydrochloric acid, sulfuric acid, and nitric acid, then salt-treated with a concentration of 0.2 - 5%, and finally calcined at high temperature and ground by a ball mill to control the final zinc oxide particles to reach 25 nanometers. Also, the sites of the wrapping substance on the surface of zinc oxide are increased, and the content of ethoxysilane or n-octyltriethoxysilane can be increased to completely wrap the surface of zinc oxide, thus isolating the contact between zinc oxide and the outside world. After the above process treatment, zinc oxide and carbomer can coexist stably for a long time; and because the particle size of zinc oxide after the above process treatment is smaller, it can ensure the dispersion effect of the sunscreen agent. Therefore, for the same added amount of zinc oxide, zinc oxide after this process treatment can obtain a higher SPF value, that is, zinc oxide after this process treatment has a better sunscreen effect. Using the formula and preparation process of this solution, the optimal effect can be achieved with the minimum amount of raw materials used, and it has good stability and is easy to operate and produce.

[0059] In addition, the present invention preferably uses a dispersion disk to treat and disperse the sunscreen agent, and the dispersion effect is better than that of an ordinary stirring paddle.

[0060] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to explain the present invention and are not used to limit the present invention.

[0061] An epidermal 3D model is established, and the blank group NC group, the cream group without sunscreen agent and extract group (compared with the SSE sunscreen group without adding sunscreen agent and sunflower bud extract group) - Vehicle group, the sunscreen group without extract - sunscreen group (compared with the SSE sunscreen group without adding sunflower bud extract group), and the sunscreen group containing extract and sunscreen agent - SSE sunscreen group are set respectively, and then the corresponding data of each group under different test conditions are compared.

[0062] Among them, the preparation method of the sunscreen containing extract and sunscreen agent corresponding to the SSE sunscreen group in the above groups includes the following steps:

[0063] S1. Mix 10% of the first humectant (6% butylene glycol and 4% glycerol), 0.05% of the chelating agent (disodium EDTA), 0.01% of the neutralizing agent (potassium hydroxide), 0.1% of the first thickener (carbomer), and 33.57% of the solvent (water) according to the ratio, stir evenly after mixing to obtain the A-phase component;

[0064] S2. First, mix zinc oxide with hydrochloric acid solution (1:2), then treat it with 1% sodium hydroxide with a concentration of 1%, and finally calcine it at 350 °C for 4 h, grind it, control the contact points between the surface of zinc oxide and triethoxysilane octane, and ensure that the final particle size of zinc oxide particles is 15 - 35 nm; then add deep-sea two-jointed watercress oil for dispersion, and then homogenize and disperse 25% of the dispersed zinc oxide, 0.1% of the remaining sunscreen agent (titanium dioxide), and the remaining raw materials in the B-phase component (18% emollient, 4% skin feel regulator, 5% emulsifier, and 0.05% second thickener) to obtain the B-phase component;

[0065] S3. Heat the A-phase component and the B-phase component to 80 °C respectively, mix them, homogenize them, and then add 0.7% of the third thickener (0.5% acrylate / sodium acryloyldimethyltaurate copolymer, 0.1% isocetane, and 0.1% polysorbate - 80), 0.8% of the preservative (0.4% phenoxyethanol, 0.3% triethylene glycol

[0066] and 0.1% ethylhexylglycerol), and 0.6% of the second humectant (0.5% 1,2 - hexanediol and 0.1% caprylyl glycol) for homogenization. Stir and cool down to 45 °C, then add 2.02% of the skin conditioner (0.%% sunflower bud extract

[0067] 0.1%, 0.1% Caesalpinia spinosa fruit extract, 1.4% propylene glycol, 0.4% phenoxyethanol, 0.02% sodium benzoate), stir evenly, and then obtain the above-mentioned sunscreen (sunscreen repair composition) containing extracts and sunscreen agents.

[0068] Example 1

[0069] This experiment detected the reduction of mitochondrial membrane potential intensity in epidermal cells after UV irradiation of epidermal cells (epidermal 3D model) in each of the above groups.

[0070] As Figure 1As shown, the results indicate that the sunscreen repair composition of this solution can effectively maintain the stability of mitochondrial membrane potential (MMP). As in the NC group in the figure, when exposed to UV irradiation, significant changes occurred in the mitochondrial membrane potential of epidermal cells, and mitochondrial function was damaged, which would lead to an increase in reactive oxygen species (ROS) and a decrease in ATP production, etc., severely affecting cell vital activities and even resulting in cell death. As for the Vehicle group and the sunscreen group, they also reduced the impact of UV on mitochondrial membrane potential to a certain extent. However, the membrane potential of the SSE sunscreen experimental group remained the same as before UV irradiation, indicating that the sunscreen repair composition of this solution can well maintain the mitochondrial membrane potential basically unaffected by UV, and also shows that even with a conventional sunscreen, the skin cannot be well protected. Only the SSE sunscreen experimental group can protect the mitochondria in epidermal cells from UV damage, thus ensuring that the vitality of epidermal cells is basically unaffected by UV.

[0071] Example 2

[0072] To further confirm that the membrane potential of the SSE sunscreen experimental group was not affected by UV irradiation and the function of mitochondria was not damaged by UV, the amount of ATP produced by mitochondria after UV irradiation was measured in this experiment.

[0073] As Figure 2 shown, the results indicate that the corresponding ATP production in the Vehicle group and the sunscreen group also decreased, proving that UV also damaged the mitochondria in epidermal cells in these two groups. However, there was still a certain protective effect compared to the NC group, preventing the ATP production from decreasing too much, but it did not reach the level before UV irradiation. After using the sunscreen in the SSE sunscreen experimental group, the amount of ATP produced by mitochondria in epidermal cells not only did not decrease, but instead increased several times. This shows that even with a conventional sunscreen, the skin cannot be well protected. Only this product makes the mitochondria healthier. Even under the influence of UV, the sunscreen repair composition of this solution can not only effectively protect the ATP production in the mitochondria of epidermal cells, but also significantly increase the ATP production to ensure that epidermal cells have more vigorous vitality and sufficient energy to maintain the normal operation of their life activities. When the vitality of cells is vigorous, the improvement effect on skin problems is better, reducing the death of epidermal cells, inhibiting the occurrence of inflammation, reducing skin redness, and having the long-term effects of repairing and sunscreen to protect the skin.

[0074] Example 3

[0075] To confirm that the membrane potential of the experimental group using SSE sunscreen was not affected by UV irradiation, the function of the cells was not damaged by UV, and the vitality of the cells was stronger, this experiment further measured the antioxidant capacity of mitochondria after UV irradiation.

[0076] As Figure 3 shown in the figure, the results show that: after UV irradiation, the reactive oxygen species (ROS) in the Vehicle group and the NC group increased significantly. Even in the sunscreen group containing sunscreen agents, the corresponding mitochondrial reactive oxygen species also increased significantly, which also confirmed that even when using conventional sunscreen, ultraviolet rays can still oxidize and damage the skin, resulting in wrinkles and other skin aging problems. In the SSE sunscreen experimental group of this protocol, even after UV irradiation, the level of reactive oxygen species (ROS) was basically the same as that without UV irradiation, indicating that the antioxidant capacity of its epidermal cells was still equivalent to that of healthy skin cells before UV irradiation even after UV irradiation, proving that the epidermal cells in this group had healthy vitality, and the sunscreen repair composition of this protocol could effectively inhibit the damage of free radicals caused by ultraviolet rays to cells to ensure that the health and normal life activities of the skin were not affected by ultraviolet rays.

[0077] Example 4

[0078] In this experiment, the anti-inflammatory ability of each group was studied by detecting the amount of IL-1β.

[0079] As Figure 4 shown in the figure, the results show that: since the Vehicle group and the NC group did not use any sunscreen components, the concentration of the inflammatory factor IL-1β in these two groups increased by nearly 3 times. In the sunscreen experimental group using sunscreen agents, the concentration of its inflammatory factor IL-1β also doubled. Only the SSE sunscreen experimental group was at the same concentration as before without UV irradiation, and compared with the NC group after UV irradiation, the concentration of IL-1β was significantly reduced. Therefore, it shows that even in the sunscreen experimental group using sunscreen agents, a large amount of inflammation will still occur after UV irradiation, and the sunscreen repair composition of this protocol can effectively control the inflammatory factor IL-1β and reduce a series of inflammatory reactions and infections caused by it in the nervous system, thereby improving the anti-inflammatory and repair effects of the skin.

[0080] In summary, the sunscreen repair composition of this protocol not only protects the membrane potential of mitochondria from being affected by UV irradiation and the function of mitochondria from being damaged by UV, but also enhances the function of mitochondria to produce ATP, and the life activity ability and immune ability of cells will also be enhanced.

[0081] Example 5

[0082] The UV-Vis absorbance of each group was analyzed in this experiment. Specifically, 5 mg each of the Vehicle group, SSE sunscreen group, and sunscreen control group were dissolved in 1 mL of NaOH, and the UV absorbance of the solution was measured using a UV-Vis spectrophotometer.

[0083] As Figure 5 shown, the results indicate that: The SSE sunscreen experimental group has a better absorption effect on sunlight, especially for ultraviolet rays in the range of 240 - 400 nm; compared with the Vehicle group and the sunscreen group, this group has a better absorption effect on ultraviolet rays, and the absorption rate of the Vehicle group has increased compared to other groups. This shows that the sunscreen repair composition of this scheme improves the protection of sunscreen products against ultraviolet rays, thereby protecting the dermis of the skin from damage, reducing the damage of ultraviolet rays to elastic fibers and collagen fibers, and reducing skin problems such as sunburn and sunspots, with a better sunscreen effect.

[0084] Example 6

[0085] A preparation method of a sunscreen repair composition, comprising the following preparation steps:

[0086] A preparation method of a sunscreen containing extracts and sunscreen agents, comprising the following steps: S1. According to the ratio, 7% of the first moisturizer (4% butylene glycol and 3% glycerol), 0.05% of the chelating agent (EDTA disodium), 0.01% of the neutralizing agent (potassium hydroxide), 0.1% of the first thickening agent (carbomer), and the solvent (water)

[0087] 36.89% are mixed and stirred evenly to obtain the A-phase component;

[0088] S2. Zinc oxide is first mixed with an acid solution, then treated with 1% of potassium hydroxide with a concentration of 2%, and finally calcined at 300 °C for 5 h, ground, controlling the contact points between the surface of zinc oxide and triethoxysilane octane, and ensuring that the final particle size of zinc oxide particles is 15 - 35 nm; then add deep-sea two-jointed cress oil for dispersion, and then 25% of the dispersed zinc oxide, 0.1% of the remaining sunscreen agent (titanium dioxide), and the remaining raw materials in the B-phase component (16% emollient, 5% skin feel regulator, 6% emulsifier, and 0.05% second thickening agent) are homogenized and dispersed to obtain the B-phase component;

[0089] S3. The A-phase component and the B-phase component are heated to 70 °C respectively and then mixed. After homogenization, 0.4% of the third thickening agent (0.2% acrylate / acryloyldimethyltaurates copolymer, 0.1% isocetane, and 0.1% polysorbate - 80), the preservative (0.5% phenoxyethanol, 0.2% triethylene glycol

[0090] Homogenize 0.8% of the first humectant (3% butanediol and 3% glycerol), 0.6% of the second humectant (0.5% 1,2 - hexanediol and 0.1% caprylyl glycol), and then cool the mixture with stirring to 45°C. Add 2% of the skin conditioner (0.1% sunflower sprout extract, 0.1% Caesalpinia spinosa fruit extract, 1.3% propylene glycol, 0.4% phenoxyethanol, 0.02% sodium benzoate, and 0.08% water). After stirring evenly, the sunscreen repair composition is obtained.

[0091] Example 7

[0092] A method for preparing a sunscreen repair composition, comprising the following preparation steps:

[0093] A method for preparing a sunscreen containing extracts and a sunscreen agent, comprising the following steps: S1. Mix 6% of the first humectant (3% butanediol and 3% glycerol), 0.05% of the chelating agent (EDTA disodium), 0.01% of the neutralizing agent (potassium hydroxide), 0.05% of the first thickening agent (carbomer), and the solvent (water)

[0094] 35.29% evenly by stirring to obtain the A - phase component;

[0095] S2. First, mix zinc oxide with an acid solution, then treat it with 3% sodium hydroxide, finally calcine it at 280°C for 6 h and grind it. Control the contact points between the surface of zinc oxide and triethoxysilane octane, and ensure that the final particle size of zinc oxide particles is 15 - 35 nm. Then add deep - sea two - joint watercress oil for dispersion. After that, homogenize and disperse 25% of the dispersed zinc oxide, 0.1% of the remaining sunscreen agent (titanium dioxide), and the remaining raw materials in the B - phase component (20% emollient, 4% skin - feel regulator, 5.3% emulsifier, and 0.05% second thickening agent) to obtain the B - phase component;

[0096] S3. Heat the A - phase component and the B - phase component to 75°C respectively, then mix them and homogenize. Then add 0.7% of the third thickening agent (0.5% acrylate / acryloyldimethyltaurate copolymer, 0.1% isocetane, and 0.1% polysorbate - 80), 0.8% of the preservative (0.4% phenoxyethanol, 0.3% triethylene glycol

[0097] and 0.1% ethylhexylglycerin), 0.6% of the second humectant (0.5% 1,2 - hexanediol and 0.1% caprylyl glycol), and homogenize. Stir and cool to 45°C, then add 2.05% of the skin conditioner (0.1% sunflower sprout extract, 0.1% Caesalpinia spinosa fruit extract, 1.4% propylene glycol, 0.4% phenoxyethanol, 0.02% sodium benzoate, 0.03% water). After stirring evenly, the sunscreen repair composition is obtained.

[0098] Comparative Example 1

[0099] All the preparation steps and parameters in this comparative example are the same as those in Example 2, except that polyhydroxystearic acid is used to disperse zinc oxide (i.e., the dispersants for dispersing zinc oxide are different, one is polyhydroxystearic acid and the other is Crambe abyssinica seed oil (active ingredient)). The actual dispersion effects corresponding to Comparative Example 1 and Example 2 are as Figure 6 shown.

[0100] As Figure 6 shown, compared with Example 2, when polyhydroxystearic acid dispersant is used to disperse zinc oxide in Comparative Example 1, the dispersion effect is not as good as that of this solution. There are many black dots on the left side of the picture using polyhydroxystearic acid, and the particle size is relatively large and very uneven, indicating that the powder has aggregated and the quantity is relatively large, which will seriously affect the sun protection effect. While on the right side of the picture, Crambe abyssinica seed oil (active ingredient) is used as the dispersant. Many uniform and fine droplets can be seen in the figure, and their particle size is much smaller than the scale of 20um, basically within 10um. The dispersion effect is significantly better than that of the left picture.

[0101] The above embodiments are only the preferred embodiments of the present invention and do not limit the patent scope of the present invention. The sunscreen repair composition mentioned in the present invention is not limited to the above several kinds. Therefore, the above embodiments cannot be regarded as a limitation to the protection scope of the present invention. Any equivalent structural transformation made by using the content of the specification of the present invention under the inventive concept of the present invention, or direct / indirect application in other related technical fields is included in the patent protection scope of the present invention.

Claims

1. A sunscreen repair composition, characterized in that, It includes skin conditioners, sunscreen agents and excipients; the skin conditioners include sunflower sprout extract.

2. The sunscreen repair composition according to claim 1, wherein The excipients include at least one of humectants, chelating agents, thickeners, neutralizing agents, emollients, skin feel regulators, emulsifiers, preservatives, humectants, skin conditioners and solvents.

3. The sun protection and repair composition according to claim 1, characterized in that, Calculated by the total weight percentage of the sunscreen repair composition, the sunscreen repair composition includes the following components: Phase A components: the first humectant 1-10%, chelating agent 0.05-0.1%, neutralizing agent 0.01-0.1%, the first thickener 0.05-0.15% and solvent 1-50%; Phase B components: emollient 1-25%, the sunscreen agent 0.2-66%, skin feel regulator 0.1-10%, emulsifier 0.15-8% and the second thickener 0.05-2%; wherein, the sunscreen agent includes zinc oxide; Phase C components: the third thickener 0.05-2%, preservative 0.8-2.3% and the second humectant 0.05-1.5%; Phase D components: the skin conditioner 1-5%.

4. The sun protection and repair composition according to claim 3, characterized in that, The sunscreen agent in the Phase B components further includes at least one of titanium dioxide, ethylhexyl methoxycinnamate, ethylhexyl salicylate, avobenzone, phenylbenzimidazole sulfonic acid, diethylamino hydroxybenzoyl hexyl benzoate, terephthalylidene dicamphor sulfonic acid, ethylhexyl salicylate, homosalate, bis-ethylhexyloxyphenol methoxyphenyl triazine, octocrylene, phenylbenzimidazole sulfonic acid, benzophenone-3, benzotriazolyl dodecyl p-cresol, ethylhexyl triazone, methylene bis-benzotriazolyl tetramethylbutylphenol and polysiloxane-15; The emollient in the Phase B components includes at least one of cyclodimethylsiloxane, isononyl isononanoate, trimethylpentaphenyl trisiloxane, diphenylsilanoxy phenyl polytrimethylsiloxane, isocetane, cyclodimethylsiloxane, C12-15 alkyl benzoate, octyldodecanol, lauryl laurate, Crambe abyssinica seed oil, phytosterols, shea butter, phytosqualane, jojoba oil, sunflower seed oil, Limnanthes alba seed oil, grape seed oil, macadamia nut oil, olive oil, palm oil, cocoa butter, jojoba butter, glycolipids, octyldodecanol, caprylic / capric triglyceride and phytosterols; The skin feel regulator in the Phase B components includes at least one of silica, behenyl alcohol, polymethylsilsesquioxane, dimethicone / vinyl dimethicone crosspolymer, methyl methacrylate crosspolymer, lauroyl lysine and distearyldimethylammonium chloride; The emulsifier in the B-phase component includes at least one of polyglyceryl-3 polyricinoleate, polysorbate-80, cetearyl alcohol, cocoyl glucoside, glyceryl stearate, polyglyceryl-3 diisostearate, glyceryl-2 triisostearate, polyglyceryl 2-dipolyhydroxystearate, methyl glucoside sesquistearate, PEG-20 methyl glucoside sesquistearate, potassium cetyl phosphate, cetearyl olivate (and) sorbitan olivate, disodium lauroyl glutamyl lysine, polysorbate 60, polyglyceryl-10 stearate, and PEG-100 stearate; The second thickener in the B-phase component includes stearic acid.

5. A sunscreen repair composition according to claim 3, characterized in that, The first humectant in the A-phase component includes at least one of butanediol, propylene glycol, and glycerol; the chelating agent in the A-phase component includes disodium EDTA; the neutralizing agent in the A-phase component includes at least one of potassium hydroxide, sodium hydroxide, or TEA; the first thickener in the A-phase component includes carbomer or xanthan gum; the solvent in the A-phase component includes water.

6. The sun protection and repair composition according to claim 3, characterized in that, The third thickener in the C-phase component includes at least one of sodium acrylate / sodium acryloyldimethyl taurate copolymer and polyacrylamide; The preservative in the C-phase component includes at least one of phenoxyethanol, triethylene glycol, and ethylhexylglycerin; The second humectant in the C-phase component includes at least one of 1,2-hexanediol and caprylyl glycol.

7. The sunscreen repair composition according to claim 3, wherein, The skin conditioner in the D-phase component further includes at least one of Caesalpinia spinosa fruit extract, propylene glycol, phenoxyethanol, sodium benzoate, and water.

8. A method for preparing a sunscreen repair composition according to any one of claims 3-7, characterized in that, Comprising the following steps: S1. Mix the raw materials of the A-phase component according to the ratio and stir evenly to obtain the A-phase component; S2. First mix the sunscreen agent - zinc oxide in the B-phase component with an acid solution, then treat it with sodium hydroxide or potassium hydroxide with a concentration of 0.2 - 5%, and finally calcine it at 100 - 400 °C for 1 - 10 h. After grinding, the particle size of the obtained zinc oxide particles is 15 - 35 nm; then add Crambe abyssinica seed oil for dispersion, and then homogenize and disperse the dispersed zinc oxide, the remaining sunscreen agent, and the remaining raw materials in the B-phase component to obtain the B-phase component; S3. Heat the A-phase component and the B-phase component to 65 - 80 °C respectively, mix them, homogenize them, then add the raw materials of the C-phase component for homogenization, stir and cool down to 40 - 50 °C, and then add the raw materials of the D-phase component and stir evenly to obtain the sunscreen repair composition.

9. The preparation method of a sunscreen repair composition according to claim 8, characterized in that, The acid solution is at least one of hydrochloric acid solution, sulfuric acid solution, or nitric acid solution.

10. Use of the sunscreen repair composition according to any one of claims 1-7, characterized in that, The sunscreen repair composition is used to maintain the stability of the mitochondrial membrane potential.

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