A ganoderma lucidum spore oil composition for enhancing immunity and a preparation method thereof

Abstract

The present application belongs to the field of biotechnology, and particularly relates to a ganoderma lucidum spore oil composition and a preparation method thereof. The ganoderma lucidum spore oil composition comprises the following raw materials in parts by weight: ganoderma lucidum spore oil 75-85 parts, radix codonopsis polysaccharide 4-8 parts, caput medusae chromone 5-10 parts, lecithin 2-5 parts, and stabilizer 5-8 parts. The ganoderma lucidum spore oil composition can improve the antioxidant capacity of ganoderma lucidum spore oil, maintain long-term stability of the composition, and further help to exert the effect of enhancing immunity.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, specifically relating to a Ganoderma lucidum spore oil composition that helps enhance immunity and its preparation method. Background Technology

[0002] Sub-health, a key node in the clinical health-disease continuum, is characterized by chronic fatigue syndrome (CFS), a significant decline in the vitality index (VPI), and an imbalance in immune system homeostasis. Epidemiological surveys show that over 75% of the global population is in a sub-healthy state, with 92.3% of them exhibiting abnormalities in immune function-related indicators. This third state, between health and disease, constitutes a major public health challenge in modern society. Based on the medical concept of "prevention of disease," intervening in immune regulation to prevent the progression from sub-health to a pathological state has significant strategic value for disease prevention. Currently, with the continuous advancement of immunological research and the sustained increase in the number of sub-healthy individuals, the demand for drugs that can improve immune function is becoming increasingly urgent.

[0003] Ganoderma lucidum spore oil is a lipid-soluble bioactive complex extracted directionally from mature Ganoderma lucidum spores using supercritical CO2 fluid extraction technology. It mainly contains triterpenoids (ganoderic acid AG), sterols (ergosterol), unsaturated fatty acids (ω-6 / ω-3 polyunsaturated fatty acids), and immunopolysaccharides (β-1,3-glucan), among other active ingredients. Pharmacological studies have shown that this extract can enhance macrophage phagocytic activity by activating the NF-κB signaling pathway, and its immunomodulatory effect is dose-dependent.

[0004] Despite the significant medicinal potential of Ganoderma lucidum spore oil, its physical form still faces three major technical bottlenecks: 1) the double bonds of unsaturated fatty acids are easily oxidized; 2) the liquid lipid system has poor stability; and 3) its bioavailability is insufficient in individuals with insufficient bile secretion. These problems severely restrict its clinical efficacy. Therefore, developing novel Ganoderma lucidum spore oil formulations with strong oxidative stability, high absorption efficiency, and long-lasting effects has become a crucial technical challenge in the field of immunomodulatory agents. Summary of the Invention

[0005] In order to overcome the shortcomings of the prior art, the first objective of the present invention is to provide a Ganoderma lucidum spore oil composition that helps to enhance immunity. This Ganoderma lucidum spore oil composition can improve the antioxidant capacity of Ganoderma lucidum spore oil, maintain the long-term stability of the composition, and thus help it exert its ability to enhance immunity.

[0006] The second objective of this invention is to provide a method for preparing a Ganoderma lucidum spore oil composition that helps enhance immunity.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] A Ganoderma lucidum spore oil composition that helps enhance immunity, the composition comprising the following raw materials in parts by weight: 75-85 parts of Ganoderma lucidum spore oil, 4-8 parts of Codonopsis pilosula polysaccharide, 5-10 parts of Artemisia capillaris chromogenone, 2-5 parts of lecithin, and 5-8 parts of stabilizer.

[0009] Furthermore, the composition comprises the following raw materials in parts by weight: 80 parts of Ganoderma lucidum spore oil, 7 parts of Codonopsis pilosula polysaccharide, 8 parts of Artemisia capillaris chromogenone, 3 parts of lecithin, and 7 parts of stabilizer.

[0010] Furthermore, the method for preparing the stabilizer includes the following steps:

[0011] (1) Add dopamine hydrochloride to Tris-HCl buffer to obtain a mixture; add selenium nanoparticles to the mixture for reaction, centrifuge, collect the solids and wash to obtain polydopamine-coated selenium nanoparticles.

[0012] (2) Disperse polydopamine-coated selenium nanoparticles in water, then add rosmarinic acid, adjust the pH, stir, centrifuge, wash, and dry to obtain a stabilizer.

[0013] Further, the concentration of dopamine hydrochloride in the mixture in step (1) is 0.8 to 1.2 mg / mL; the pH of the Tris-HCl buffer is 8.0 to 9.0.

[0014] Further, in step (1), the mass ratio of the nano-selenium particles to dopamine hydrochloride is (1-2):1; and the reaction time is 8-12 h.

[0015] Further, in step (2), the mass ratio of polydopamine-coated selenium nanoparticles to rosmarinic acid is (3-5):1; the pH value is 2-3; and the stirring time is 4-5 h.

[0016] Furthermore, the dosage form of the composition is a soft capsule; the capsule shell of the soft capsule comprises gelatin, glycerin and water.

[0017] The preparation method of the above-mentioned Ganoderma lucidum spore oil composition that helps enhance immunity includes the following steps:

[0018] (a) Ganoderma lucidum spore oil, Codonopsis pilosula polysaccharide, Artemisia capillaris chromogenone, lecithin and stabilizer are mixed and cut in proportion to obtain spore core liquid;

[0019] (b) Mix water and glycerin and heat to 65-75°C. Add gelatin, stir and vacuum under sealed conditions, and then let stand to obtain capsule shell gel solution.

[0020] (c) The core material liquid is injected into the capsule skin made of capsule skin glue under an environment of 18-26℃, and after pressing, shaping, washing and drying, the Ganoderma lucidum spore oil composition is obtained.

[0021] Further, the mass ratio of water, glycerin and gelatin in step (b) is 100:(45-50):(110-115).

[0022] Further, in step (c), the filling amount of the core material liquid is 0.3-0.6 g / capsule; the shaping temperature is 20-25°C; and the mass ratio of the core material liquid to the capsule shell adhesive liquid is 100:(27-53).

[0023] The beneficial technical effects of this invention are as follows:

[0024] 1. This invention provides a Ganoderma lucidum spore oil composition that helps enhance immunity, comprising Ganoderma lucidum spore oil and stabilizers. The stabilizer is prepared by coating nano-selenium particles with polydopamine and the antioxidant rosmarinic acid. Nano-selenium itself possesses excellent antioxidant capabilities, while rosmarinic acid, as a potent antioxidant, forms a stable complex with dopamine. Adding it as a stabilizer to the composition enhances the antioxidant properties of Ganoderma lucidum spore oil, maintains its long-term stability, and helps it exert its ability to enhance immunity. Furthermore, it supplements the body with essential selenium, further enhancing its health benefits. In addition, this invention also combines Codonopsis pilosula polysaccharides and Artemisia capillaris chromogens with Ganoderma lucidum spore oil, further enhancing immunity while effectively promoting bile secretion, thereby accelerating the body's absorption of Ganoderma lucidum spore oil and further enhancing its efficacy.

[0025] 2. The present invention also provides a method for preparing the above-mentioned Ganoderma lucidum spore oil composition that helps enhance immunity. The raw materials used in the preparation method are inexpensive, the preparation process is simple, and it is conducive to large-scale production. Detailed Implementation

[0026] The following is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention. Specific conditions not specified in the embodiments are performed according to conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, all reagents or instruments used are conventional products obtained through commercial channels.

[0027] (I) Implementation Examples

[0028] Example 1

[0029] Example 1 provides a Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following raw materials in parts by weight: 80 parts of Ganoderma lucidum spore oil, 6 parts of Codonopsis pilosula polysaccharide, 8 parts of Artemisia capillaris chromogenone, 3 parts of lecithin, and 7 parts of stabilizer.

[0030] The method for preparing the stabilizer includes the following steps:

[0031] (1) Dopamine hydrochloride was added to Tris-HCl buffer solution with pH=8.5 to obtain a mixture with a dopamine hydrochloride concentration of 1 mg / mL. Then, 120 nm nano-selenium particles were added to the mixture and ultrasonically dispersed to make the nano-selenium particles evenly distributed. The mass ratio of nano-selenium particles to dopamine hydrochloride was 1.5:1. After reacting for 10 h, the reaction solution was centrifuged, the solid was collected and washed to obtain polydopamine-coated nano-selenium particles.

[0032] (2) According to the mass ratio of polydopamine-coated selenium nanoparticles to rosmarinic acid of 4:1, polydopamine-coated selenium nanoparticles and rosmarinic acid were added to water, and then the pH was adjusted to 2.5 and stirred for 5 hours to promote the adsorption of rosmarinic acid by dopamine. After stirring, the stabilizer was obtained by centrifugation, washing and drying.

[0033] Example 1 also provides a method for preparing the above-mentioned Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following steps:

[0034] (a) Ganoderma lucidum spore oil, Codonopsis pilosula polysaccharide, Artemisia capillaris chromogenone, lecithin and stabilizer are mixed evenly according to the above weight proportions, and the mixture is sheared at 3000 rpm for 8 minutes to obtain the core material liquid;

[0035] (b) After mixing water and glycerin evenly, heat to 70°C, add gelatin, stir evenly under sealed conditions, remove air bubbles by vacuuming, and then let stand to obtain capsule shell gel solution; wherein the mass ratio of water, glycerin and gelatin is 25:12:28;

[0036] (c) 0.4 g / capsule of core material liquid is quantitatively injected into the capsule skin made by pressure injection of capsule skin glue at 24°C, and then pressed into capsules and shaped at 23°C. After washing and drying, Ganoderma lucidum spore oil composition is obtained, wherein the mass ratio of core material liquid to capsule skin glue liquid is 5:2.

[0037] Example 2

[0038] Example 2 provides a Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following raw materials in parts by weight: 75 parts Ganoderma lucidum spore oil, 4 parts Codonopsis pilosula polysaccharide, 5 parts Artemisia capillaris chromogenone, 2 parts lecithin, and 5 parts stabilizer.

[0039] The method for preparing the stabilizer includes the following steps:

[0040] (1) Dopamine hydrochloride was added to Tris-HCl buffer solution with pH=8 to obtain a mixture with a concentration of 0.8 mg / mL of dopamine hydrochloride. Then, 100 nm nano-selenium particles were added to the mixture and ultrasonically dispersed to make the nano-selenium particles evenly distributed. The mass ratio of nano-selenium particles to dopamine hydrochloride was 1:1. After reacting for 8 h, the reaction solution was centrifuged, the solid was collected and washed to obtain polydopamine-coated nano-selenium particles.

[0041] (2) According to the mass ratio of polydopamine-coated selenium nanoparticles to rosmarinic acid of 3:1, polydopamine-coated selenium nanoparticles and rosmarinic acid were added to water, and then the pH was adjusted to 2 and stirred for 4 hours to promote the adsorption of rosmarinic acid by dopamine. After stirring, the stabilizer was obtained by centrifugation, washing and drying.

[0042] Example 2 also provides a method for preparing the above-mentioned Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following steps:

[0043] (a) Ganoderma lucidum spore oil, Codonopsis pilosula polysaccharide, Artemisia capillaris chromogenone, lecithin and stabilizer are mixed evenly according to the above weight proportions, and the mixture is sheared at 4000 rpm for 5 min to obtain the core material liquid;

[0044] (b) After mixing water and glycerin evenly, heat to 65°C, add gelatin, stir evenly under sealed conditions, remove air bubbles by vacuuming, and then let stand to obtain capsule shell gel solution; wherein the mass ratio of water, glycerin and gelatin is 20:9:22.

[0045] (c) 0.3 g / capsule of core material liquid is quantitatively injected into the capsule skin made by pressure injection of capsule skin glue at 18°C, and then pressed into capsules and shaped at 20°C. After washing, drying and other processes, Ganoderma lucidum spore oil composition is obtained, wherein the mass ratio of core material liquid to capsule skin glue liquid is 100:53.

[0046] Example 3

[0047] Example 3 provides a Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following raw materials in parts by weight: 85 parts Ganoderma lucidum spore oil, 8 parts Codonopsis pilosula polysaccharide, 10 parts Artemisia capillaris chromogenone, 5 parts lecithin, and 8 parts stabilizer.

[0048] The method for preparing the stabilizer includes the following steps:

[0049] (1) Dopamine hydrochloride was added to Tris-HCl buffer solution with pH=9 to obtain a mixture with a concentration of 1.2 mg / mL of dopamine hydrochloride. Then, 150 nm nano-selenium particles were added to the mixture and ultrasonically dispersed to make the nano-selenium particles evenly distributed. The mass ratio of nano-selenium particles to dopamine hydrochloride was 2:1. After reacting for 12 h, the reaction solution was centrifuged, the solid was collected and washed to obtain polydopamine-coated nano-selenium particles.

[0050] (2) According to the mass ratio of polydopamine-coated selenium nanoparticles to rosmarinic acid of 5:1, the polydopamine-coated selenium nanoparticles and rosmarinic acid were added to water, and then the pH was adjusted to 3 and stirred for 5 hours to promote the adsorption of rosmarinic acid by dopamine. After stirring, the stabilizer was obtained by centrifugation, washing and drying.

[0051] Example 3 also provides a method for preparing the above-mentioned Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following steps:

[0052] (a) Ganoderma lucidum spore oil, Codonopsis pilosula polysaccharide, Artemisia capillaris chromogenone, lecithin and stabilizer are mixed evenly according to the above weight proportions, and the mixture is sheared at 5000 rpm for 8 minutes to obtain the core material liquid.

[0053] (b) After mixing water and glycerin evenly, heat to 75°C, add gelatin, stir evenly under sealed conditions, remove air bubbles by vacuuming, and then let stand to obtain capsule shell gel solution; wherein the mass ratio of water, glycerin and gelatin is 20:10:23.

[0054] (c) 0.6 g / capsule of core material liquid is quantitatively injected into the capsule skin made by pressure injection of capsule skin glue at 26°C, and then pressed into pellets and shaped at 25°C. After washing and drying, Ganoderma lucidum spore oil composition is obtained, wherein the mass ratio of core material liquid to capsule skin glue liquid is 100:27.

[0055] (II) Comparative Example

[0056] Comparative Example 1

[0057] Comparative Example 1 is basically the same as Example 1, except that the stabilizer in Example 1 is replaced with an equal amount of nano-selenium particles.

[0058] Comparative Example 2

[0059] Comparative Example 2 is basically the same as Example 1, except that step (2) in the stabilizer preparation method of Example 1 is omitted.

[0060] Comparative Example 3

[0061] Comparative Example 3 is basically the same as Example 1, except that the stabilizer in Example 1 is replaced with a mixture of nano-selenium particles and rosmarinic acid, and the amount of nano-selenium particles and rosmarinic acid is the same as in Example 1.

[0062] Comparative Example 4

[0063] Comparative Example 4 is basically the same as Example 1, except that it does not contain the artemisia chromogenin from Example 1.

[0064] (III) Test Examples

[0065] Experimental Example 1

[0066] Accelerated stability tests were conducted on the Ganoderma lucidum spore oil compositions prepared in Examples 1 and Comparative Examples 1-4. The specific method was as follows: the Ganoderma lucidum spore oil compositions prepared in each group were sealed in self-sealing bags and placed in a stability test chamber at 37°C and 75% relative humidity. The peroxide values ​​of each group were tested at 0, 1 and 3 months respectively, according to GB 5009.227-2023 "Determination of Peroxide Value in Food". The results are shown in Table 1.

[0067] Table 1 Oxidative Stability Test of Products

[0068]

[0069] As shown in Table 1, the peroxide value of the composition obtained in Example 1 is significantly lower than that of Comparative Examples 1 to 4, which indicates that the Ganoderma lucidum spore oil composition of the present invention has excellent stability.

[0070] Comparative Example 1 replaced the stabilizer with nano-selenium particles, Comparative Example 2 only coated the surface of nano-selenium with polydopamine, and Comparative Example 3 simply mixed nano-selenium and rosmarinic acid directly. The peroxide values ​​of Comparative Examples 1-3 were all significantly higher than those of Example 1, indicating that the stabilizer of the present invention can effectively improve the stability of Ganoderma lucidum spore oil. Analysis reveals that the stabilizer consists of rosmarinic acid and dopamine coated with nano-selenium particles. Nano-selenium itself possesses excellent antioxidant capabilities, while rosmarinic acid, as a potent antioxidant, forms a stable complex with dopamine. Adding it as a stabilizer to the composition can significantly improve the antioxidant properties of Ganoderma lucidum spore oil and maintain its long-term stability.

[0071] Experimental Example 2

[0072] The immune-enhancing effects of the samples prepared in Example 1 and Comparative Examples 1-4 were tested.

[0073] 1. Laboratory animals, grouping, and dosing regimen:

[0074] Sixty SPF-grade male Kunming mice, weighing 18–22 grams, were selected and divided into two groups: Immunization Group I and Immunization Group II. NK cell activity was measured in Immunization Group I, and the number of antibody-producing cells was measured in Immunization Group II. Both Immunization Group I and Immunization Group II included low, medium, and high dose groups (Example 1), comparative groups 1–4, and a blank control group, with 10 mice in each group.

[0075] In Example 1, the dosages for the low, medium, and high dose groups were 0.20, 0.40, and 1.20 g / kg / day, respectively (equivalent to 5, 10, and 30 times the recommended human dose). The dosage for comparative groups 1-4 was 1.20 g / kg / day, and the blank control group received an equal volume of water. The medication was administered orally once daily, with a gavage volume of 0.1 mL / 10 g mouse weight. Immune indicators were measured after 33 consecutive days of gavage.

[0076] 2. Experimental environmental conditions:

[0077] The experimental conditions were a barrier environment with a temperature of 22–24°C and a humidity of 52–58%.

[0078] 3. Experimental Methods

[0079] 3.1 Assay for NK cell activity (lactate dehydrogenase assay)

[0080] After drug administration, mice in each group were euthanized by cervical dislocation, and their spleens were aseptically removed and placed in a small petri dish containing an appropriate amount of Hank's solution. The spleens were ground to prepare a single-cell suspension, filtered through a 200-mesh sieve, washed twice with Hank's solution, and centrifuged for 10 min each time (1000 rpm). The supernatant was discarded, the cytoplasm was sprinkled, 0.5 mL of sterile water was added for 20 seconds, centrifuged again, the supernatant was discarded, and erythrocyte lysis buffer was added. After lysing the erythrocytes, 0.5 mL of twice-strength Hank's solution and 8 mL of Hank's solution were added, and the mixture was centrifuged at 1000 rpm for 10 min. The cells were resuspended in 1 mL of RPMI 1640 complete culture medium containing 10% fetal bovine serum, stained with tyrosine blue, and counted (the viable cell count should be above 95%). The cell concentration was adjusted to 2 × 10⁶ cells / mL. 7 Cells / mL are considered effector cells; YAC-1 cells that have grown well after 24 hours of passage are taken and their concentration is adjusted to 4 × 10⁶ cells / mL using RPM11640 complete culture medium. 5The target cells were 100 μL each of target cells and effector cells (50:1 effector-to-target ratio) and added to a U-shaped 96-well plate. 100 μL each of target cells and culture medium were added to the spontaneous release wells, and 100 μL each of target cells and 2.5% Triton were added to the maximum release wells. All of the above were set up in triplicate. The plates were incubated at 37°C in a 5% CO2 incubator for 4 h. The 96-well plates were then centrifuged at 1500 r / min for 5 min. 100 μL of supernatant was collected from each well and placed in a flat-bottomed 96-well plate. 100 μL of LDH matrix solution was added simultaneously. The plates were reacted at room temperature for 5 min, and 30 μL of 1 mol / L HCl was added to each well. The optical density (OD) was measured at 490 nm using a microplate reader. NK cell activity was calculated as: [OD of reaction wells - OD of spontaneous release wells) / (OD of maximum release wells - OD of spontaneous release wells)] × 100%. The results are shown in Table 2.

[0081] 3.2 Detection of antibody-producing cells (Jerne modified slide method)

[0082] Sheep blood was used to prepare seroconverted red blood cells (SRBCs). The SRBCs were then dissolved in physiological saline to form a 2% (v / v) cell suspension. On day 28 of the administration of drugs to mice in step 1, each mouse was immunized by intraperitoneal injection of 0.2 mL. Five days later, the mice were sacrificed, and the spleens were harvested, gently ground, and used to prepare a cell suspension with Hank's solution. The suspension was filtered through a 200-mesh sieve, washed, and centrifuged twice. Finally, the cells were resuspended in 8 mL of Hank's solution. Cells were counted, and the cell concentration was adjusted to 5 × 10⁻⁶ cells / mL. 6 Cells / mL. Dissolve the surface culture medium (1g agarose in double-distilled water to 100mL) by heating, then mix with an equal volume of twice the concentration of Hank's solution (pH 7.4). Dispense 0.5mL into small test tubes, then add 50μL of 10% (v / v) SRBC suspension and 20μL of spleen cell suspension (5×10⁻⁶ cells / mL) prepared with SA buffer to each tube. 6 Mix the sample (number of plaques per mL) quickly and pour it onto a slide that has been brushed with a thin layer of agarose. After the agarose has solidified, place the slide flat on a slide holder and incubate it in a CO2 incubator for 1.5 h. Add guinea pig serum complement diluted with SA buffer (SA buffer to guinea pig serum volume ratio 8:1) into the groove of the slide holder and continue incubation for 1.5 h. Count the number of hemolytic plaques. The results are shown in Table 3.

[0083] Table 2. Tests of mouse NK cell activity in the blank control group and each example.

[0084]

[0085]

[0086] Table 3. Determination of antibody-producing cells in mice in the blank control group and each example.

[0087] Grouping Dosage (g / kg) Number of animals (n) <![CDATA[Number of hemolytic plaques (number / 10 6 spleen cells)]]> Blank control group 0 10 104±42 Example 1 Low-dose group 0.2 10 132±37 In Example 1, the dosage group 0.4 10 141±50 Example 1 High-dose group 1.2 10 165±36 Comparative Example 1 1.2 10 119±41 Comparative Example 2 1.2 10 126±34 Comparative Example 3 Groups 1.2 10 128±36 Comparative Example 4 Groups 1.2 10 121±38

[0088] As shown in Tables 2-3, compared with the blank control group and comparative examples 1-4, the NK cell activity and antibody-producing cells in the Example 1 group were significantly improved. It was also found that with increasing dosage, the NK cell activity and antibody-producing cells in mice were significantly improved, which indicates that the Ganoderma lucidum spore oil composition fully exerted its function of enhancing immunity.

[0089] Compared to Example 1, Comparative Examples 1-3, which adjusted the preparation process of the stabilizer, showed a significant decrease in immune efficacy. This was because the stability of Ganoderma lucidum spore oil was affected, thus impacting its immune capacity. Comparative Example 4, which omitted artemisia capillaris chromogen, also showed a decrease in immune efficacy. This indicates that the combination of artemisia capillaris chromogen and Ganoderma lucidum spore oil further enhances immunity while effectively promoting bile secretion, thereby accelerating the body's absorption of Ganoderma lucidum spore oil and further enhancing its medicinal effects.

[0090] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. Based on the present invention, some modifications or substitutions can be made, but these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of protection claimed by the present invention.

Claims

1. A Ganoderma lucidum spore oil composition that helps enhance immunity, characterized in that, The composition comprises the following raw materials in parts by weight: 75-85 parts of Ganoderma lucidum spore oil, 4-8 parts of Codonopsis pilosula polysaccharide, 5-10 parts of Artemisia capillaris chromogenone, 2-5 parts of lecithin, and 5-8 parts of stabilizer; The method for preparing the stabilizer includes the following steps: (1) Add dopamine hydrochloride to Tris-HCl buffer to obtain a mixture; add selenium nanoparticles to the mixture for reaction, centrifuge, collect the solids and wash to obtain polydopamine-coated selenium nanoparticles. (2) Disperse polydopamine-coated selenium nanoparticles in water, then add rosmarinic acid, adjust the pH, stir, centrifuge, wash, and dry to obtain a stabilizer.

2. The Ganoderma lucidum spore oil composition according to claim 1, characterized in that, The composition comprises the following raw materials in parts by weight: 80 parts of Ganoderma lucidum spore oil, 7 parts of Codonopsis pilosula polysaccharide, 8 parts of Artemisia capillaris chromogenone, 3 parts of lecithin, and 7 parts of stabilizer.

3. The Ganoderma lucidum spore oil composition according to claim 1, characterized in that, The concentration of dopamine hydrochloride in the mixture described in step (1) is 0.8~1.2 mg / mL; the pH of the Tris-HCl buffer is 8.0~9.

0.

4. The Ganoderma lucidum spore oil composition according to claim 1, characterized in that, In step (1), the mass ratio of the nano-selenium particles to dopamine hydrochloride is (1~2):1; the reaction time is 8~12 h.

5. The Ganoderma lucidum spore oil composition according to claim 1, characterized in that, In step (2), the mass ratio of polydopamine-coated selenium nanoparticles to rosmarinic acid is (3~5):1; the pH value is 2~3; and the stirring time is 4~5 h.

6. The Ganoderma lucidum spore oil composition according to claim 1, characterized in that, The composition is in the form of a soft capsule; the capsule shell of the soft capsule comprises gelatin, glycerin and water.

7. The method for preparing the Ganoderma lucidum spore oil composition that helps enhance immunity according to claim 6, characterized in that, Includes the following steps: (a) Ganoderma lucidum spore oil, Codonopsis pilosula polysaccharide, Artemisia capillaris chromogenone, lecithin and stabilizer are mixed and cut in proportion to obtain core material liquid; (b) Mix water and glycerin and heat to 65-75 °C. Add gelatin, stir and vacuum under sealed conditions, and then let stand to obtain capsule shell gel solution. (c) The core material liquid is injected into the capsule skin made of capsule skin glue under an environment of 18~26 ℃, and after pressing, shaping, washing and drying, the Ganoderma lucidum spore oil composition is obtained.

8. The method for preparing the Ganoderma lucidum spore oil composition that helps enhance immunity according to claim 7, characterized in that, The mass ratio of water, glycerin and gelatin in step (b) is 100:(45~50):(110~115).

9. The method for preparing the Ganoderma lucidum spore oil composition that helps enhance immunity according to claim 7, characterized in that, The filling amount of the core material liquid in step (c) is 0.3~0.6 g / capsule; the shaping temperature is 20~25 ℃; the mass ratio of the core material liquid to the capsule shell liquid is 100:(27~53).

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