Emotional soothing plant essential oil composition and preparation method thereof

Abstract

The invention provides a plant essential oil composition for relieving emotion and a preparation method thereof, the plant essential oil composition is prepared by randomly combining and compounding eight essential oils such as lavender oil, geranium oil, lemon oil and the like, or the essential oils are compounded and then subjected to molecular distillation to obtain a characteristic aroma component, and the characteristic aroma component is combined with a carbonic ester bond and a non-volatile unit to prepare an ester front incense body; the glycyrrhizic acid hydrogel is prepared through glycyrrhizic acid hydrogel network embedding, and belongs to the technical field of functional spices and application thereof. According to the invention, multiple essential oil molecules are subjected to Van der Waals force and hydrophobic effect to enhance tight connection among essential oil, and synergistically exert an emotion relieving effect; or embedding is performed through a glycyrrhizic acid-Mg < 2 + >-tannic acid system, formation of active junction points among glycyrrhizic acid nanofibers is promoted through Mg < 2 + >, binding sites are enhanced, self-healing is realized and structural stability is maintained through metal coordination, in addition, Mg < 2 + > and tannic acid are chelated to form a stable metal polyphenol network, oxidative degradation of essential oil is inhibited, and stable volatilization of functional components is ensured.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional spices and their applications, and particularly relates to a mood-relief plant essential oil composition and a preparation method thereof. Background Art

[0002] With the rapid development of modern society, individuals face a significant increase in the multiple stresses of daily life, particularly challenges stemming from social interactions, a fast-paced lifestyle, and economic uncertainty. These factors often lead to emotional distress and have a significant impact on human mental health. Therefore, the search for effective methods of emotion regulation has become a hot topic in psychology and related fields. Among the many avenues for emotion regulation, olfactory aromatherapy has attracted considerable attention due to its natural, safe, and broad application prospects. Spices and fragrances are common ingredients in food, cosmetics, and everyday items, and are frequently encountered. Many essential oils have been shown to have mood-soothing effects and are used in aromatherapy for relaxation through inhalation or massage. For example, lavender essential oil is widely used to relieve anxiety and improve sleep, citrus essential oils are used to enhance feelings of well-being, and rosemary essential oil is used to reduce fatigue. However, the effectiveness of essential oils depends on their concentration. When the stimulus concentration increases, the relaxing properties of lavender essential oil increase, while the relaxing properties of lemon essential oil do not increase accordingly. The efficacy of different essential oils may have a nonlinear concentration-dependent relationship, which increases the difficulty of their use. Furthermore, the aroma characteristics of some single essential oils are relatively simple, which leads consumers to express a high degree of individuality and uniqueness in their aroma preferences. The above essential oils all contain characteristic aroma components (such as linalool), but due to their small molecular weight and high vapor pressure, their aroma release rate in an open environment is fast and the fragrance lasts for a short time. This rapid volatilization characteristic not only reduces the effectiveness of fragrance products, but may also lead to nonlinear decay of fragrance concentration over time, thereby affecting the persistence of its effect on GABA receptors and the stability of its mood regulation effect. In addition, during the application process, it still faces physical property limitations such as strong volatility and insufficient fragrance persistence. The pre-fragrance technology combines highly volatile fragrance molecules with less volatile substrates through unstable chemical bonds, and uses mild external conditions (such as light, temperature, enzymatic hydrolysis or pH changes) to trigger the breaking of chemical bonds, thereby achieving the slow release of fragrance. This technology can significantly extend the durability of fragrances and effectively solve the problem of short fragrance lasting time of traditional fragrances due to high volatility. Therefore, it is possible to develop a plant compound essential oil that has both soothing effects and long-lasting fragrance and is more widely accepted to better adapt to the fragrance preferences of different users. Summary of the Invention

[0003] Technical problem to be solved: In view of the above technical problems, the object of the present invention is to provide a plant essential oil composition for relieving emotions and its preparation method. It is prepared by randomly combining and compounding 8 essential oils such as lavender oil, geranium oil, lemon oil, etc., or obtaining characteristic aroma components by molecular distillation after compounding the essential oils, and combining carbonate bonds with non-volatile units to prepare ester-type pre-aromas, and preparing it by embedding in a glycyrrhizic acid hydrogel network, belonging to the technical field of functional fragrances and their applications. The present invention can enhance the tight connection between essential oils through van der Waals forces and hydrophobic interactions of multiple essential oil molecules, and synergistically play a role in relieving emotions; or through embedding in the glycyrrhizic acid-Mg 2+ -tannic acid system, promoting the formation of active connection points between glycyrrhizic acid nanofibers through Mg 2+ , enhancing binding sites, and achieving self-healing through metal coordination to maintain structural stability. In addition, Mg 2+ chelates with tannic acid to form a stable metal polyphenol network, inhibiting the oxidative degradation of essential oils and ensuring the stable volatilization of active ingredients.

[0004] Technical solution: A plant essential oil composition for relieving emotions, which is composed of two or more of the following essential oils: lavender oil, geranium oil, lemon oil, purple peppermint oil, rosemary oil, Jinshan rose essential oil, osmanthus essential oil, Roman chamomile essential oil. A preparation method of a plant essential oil composition for relieving emotions, comprising the following steps: mixing a variety of essential oil components evenly to prepare a plant essential oil composition A. Furthermore, it also includes the following steps: Step 1. Extracting characteristic aroma components and the remaining essential oil components from the plant essential oil composition A by molecular distillation, and performing precursor treatment on the characteristic aroma components to obtain pre-aromas; Step 2. Embedding the pre-aromas and the remaining essential oil components with the glycyrrhizic acid-Mg 2+ -tannic acid system, and then performing microemulsification treatment to obtain a plant essential oil composition B. Furthermore, the characteristic aroma components in the step 1 include linalool, geraniol, citronellol, menthol. Furthermore, the precursor treatment in the step 1 is to generate ester-type pre-aromas of characteristic aroma components with carbonate substances. Furthermore, the preparation method of the glycyrrhizic acid-Mg 2+ -tannic acid system in the step 2: ① Dissolving magnesium chloride in water to make a magnesium chloride solution with a concentration of 15-20 g / L, and then adding 3-6 wt% glycyrrhizic acid to the magnesium chloride solution, and slowly stirring at 50-70 °C to obtain a glycyrrhizic acid-Mg 2+ solution; ② Making a tannic acid solution by adding water to tannic acid, and mixing it evenly with the glycyrrhizic acid-Mg 2+ solution to obtain a glycyrrhizic acid-Mg2+ - Tannic acid solution, namely glycyrrhizic acid-Mg 2+ - Tannic acid system. Furthermore, the concentration of tannic acid in ② is 15 - 35 g / L; the volume ratio of tannic acid solution to glycyrrhizic acid-Mg 2+ solution is (1 - 3):(1 - 4). Furthermore, the conditions for microemulsification treatment in step 2 are: treatment temperature 25 - 35°C, stirring speed 6000 - 9000 r / min, treatment time 40 - 60 min. Use of the above-mentioned plant essential oil composition or the plant essential oil composition prepared by any one of the methods in the preparation of a product for soothing emotions. Furthermore, in the aroma-smelling state of the product for soothing emotions, the AUC of theta waves in the frontal lobe of the brain is significantly higher than that in the resting state. Beneficial effects: 1. The present invention randomly combines and blends lavender oil, geranium oil, lemon oil, purple peppermint oil, rosemary oil, golden mountain rose essential oil, osmanthus essential oil, and roman chamomile essential oil to prepare a plant essential oil composition with the efficacy of soothing emotions. It can utilize the van der Waals force and hydrophobic interaction between various plant essential oils to enhance the tight connection between essential oils and synergistically play the role of soothing emotions; it can also obtain characteristic aroma components (such as linalool, geraniol, citronellol, menthol, etc.) by molecular distillation of the plant essential oil composition and prepare a pre-aroma body. The characteristic aroma components and non-volatile units are combined through carbonate bonds to form an ester pre-aroma body, which is embedded by glycyrrhizic acid self-assembled into a hydrogel network with a fibrous network structure to prepare a plant essential oil composition with the efficacy of soothing emotions. It can not only solve the problem of single smell and limited acceptance of single essential oils, but also extend the action time of its active ingredients and enhance the synergistic effect between active ingredients. 2. The present invention uses a glycyrrhizic acid-Mg 2+ - tannic acid system for encapsulation. Glycyrrhizic acid can self-assemble into long nanofibers in water and further form a supramolecular hydrogel with a fibrous network structure. And Mg 2+ promotes the construction of the network structure in the glycyrrhizic acid gel, can increase the number of active cross-linking points between glycyrrhizic acid nanofibers to improve the binding sites of essential oil molecules; in addition, Mg 2+ forms a reversible metal coordination interaction with the carboxyl group in glycyrrhizic acid. When the internal essential oil molecules are released by photo-response triggering, the metal coordination interaction will be disrupted. Due to the reversibility of the metal coordination interaction, self-healing will occur to maintain the stability and integrity of the outer layer structure; Mg 2+It can also provide binding sites for the chelation of metal ions with tannic acid, forming a highly stable metal-polyphenol network attached to the outermost layer of the loading structure, maintaining the antioxidant properties of polyphenolic substances, inhibiting the oxidative degradation of essential oils, and ensuring the volatilization of the active ingredients in the plant essential oil composition. 3. The plant essential oil composition prepared by the present invention releases active ingredients, and realizes mood soothing at the neurophysiological level by synergistically activating the mood regulation brain network (such as the anterior cingulate gyrus and the medial frontal lobe), enhancing the theta wave activity in the prefrontal lobe, and inhibiting the metabolic activity in the right prefrontal lobe to activate the parasympathetic nerve. 4. The plant essential oil composition prepared by the present invention has the characteristics of rich aroma, harmonious pleasure, and stable and long-lasting fragrance retention. It can accurately and controllably exert the mood regulation effect in a specific occasion, improve the overall mood regulation effect of the plant essential oil composition, make it more widely applicable to different people, meet the needs of users for diverse aroma experiences, and enhance the market adaptability of the product. Description of the Drawings Figure 1 For the sensory evaluation of Examples 1-5; Figure 2 For the sensory evaluation of Comparative Examples 1-8; Figure 3 For the results of the short-form POMS mood scale in Example 10; Figure 4 For the average AUC of PSD in different brain regions under resting and smelling conditions in Example 10; Figure 5 For the average AUC of PSD in different frequency bands of the frontal lobe under resting and smelling conditions in Example 10; Figure 6 For the source localization activation difference under smelling condition compared with resting condition in Example 10; Figure 7 For the comparison of the average oxyhemoglobin concentration in the prefrontal cortex under smelling and resting conditions in Example 10. Detailed Embodiments The present invention will be further described below in conjunction with embodiments. The following embodiments are explanations of the present invention and the present invention is not limited to the following embodiments: Example 1 A preparation method of a mood-soothing plant essential oil composition, comprising the following steps: S1. Mix 10 parts of geranium essential oil, 40 parts of lavender essential oil, 20 parts of lemon essential oil and 5 parts of rosemary essential oil evenly to obtain plant essential oil composition A1. Example 2 A preparation method of a mood-soothing plant essential oil composition, comprising the following steps: S1. Mix 10 parts of geranium essential oil, 40 parts of lavender essential oil, 20 parts of lemon essential oil, 5 parts of rosemary essential oil and 5 parts of Rosa banksiae 'Lutea' essential oil evenly to obtain plant essential oil composition A2. Example 3 A preparation method of a plant essential oil composition for soothing emotions, comprising the following steps: S1. Mix 10 parts of geranium essential oil, 40 parts of lavender essential oil, 20 parts of lemon essential oil, 5 parts of rosemary essential oil, 5 parts of Rosa banksiae 'Lutea' essential oil and 4 parts of Mentha piperita L. var. piperascens Malinv. et Loisel. essential oil evenly to obtain plant essential oil composition A3. Example 4 A preparation method of a plant essential oil composition for soothing emotions, comprising the following steps: S1. Mix 10 parts of geranium essential oil, 40 parts of lavender essential oil, 20 parts of lemon essential oil, 5 parts of rosemary essential oil, 5 parts of Rosa banksiae 'Lutea' essential oil, 4 parts of Mentha piperita L. var. piperascens Malinv. et Loisel. essential oil and 4 parts of Osmanthus fragrans Lour. essential oil evenly to obtain plant essential oil composition A4. Example 5 A preparation method of a plant essential oil composition for soothing emotions, comprising the following steps: S1. Mix 10 parts of geranium essential oil, 40 parts of lavender essential oil, 20 parts of lemon essential oil, 5 parts of rosemary essential oil, 5 parts of Rosa banksiae 'Lutea' essential oil, 4 parts of Mentha piperita L. var. piperascens Malinv. et Loisel. essential oil, 4 parts of Osmanthus fragrans Lour. essential oil and 2 parts of Anthemis nobilis L. essential oil evenly to obtain plant essential oil composition A5. Comparative Example 1 The difference between this comparative example and Example 5 is that only lavender essential oil is used. Comparative Example 2 The difference between this comparative example and Example 5 is that only lemon essential oil is used. Comparative Example 3 The difference between this comparative example and Example 5 is that only geranium essential oil is used. Comparative Example 4 The difference between this comparative example and Example 5 is that only rosemary essential oil is used. Comparative Example 5 The difference between this comparative example and Example 5 is that only Rosa banksiae 'Lutea' essential oil is used. Comparative Example 6 The difference between this comparative example and Example 5 is that only Mentha piperita L. var. piperascens Malinv. et Loisel. essential oil is used. Comparative Example 7 The difference between this comparative example and Example 5 is that only Osmanthus fragrans Lour. essential oil is used. Comparative Example 8 The difference between this comparative example and Example 5 is that only Anthemis nobilis L. essential oil is used. Performance determination (1) Sensory evaluation Thirty professionals (15 males and 15 females) were selected to conduct sensory evaluations on Examples 1-5 and Comparative Examples 1-8, and five indicators of their aroma, namely "harmony", "richness", "pleasantness", "likability", and "soothing degree", were scored on a scale of 1-9 points. It can be seen from Figure 1 and Figure 2 that the harmony, richness, pleasantness, likability, and soothing degree of the plant essential oil compositions prepared in Examples 1-5 are all higher than those in Comparative Examples 1-8. In particular, the sensory evaluation of Example 5 is high, indicating that compared with the single essential oils in Comparative Examples 1-8, the combined use of multiple plant essential oils can utilize the van der Waals force and hydrophobic interaction between essential oil molecules to strengthen the mutual synergy between essential oil molecules, thereby improving the soothing effect of plant essential oil compositions. Example 6 A preparation method of a plant essential oil composition for soothing emotions, comprising the following steps: S1. The plant essential oil composition A1 prepared in Example 1 was subjected to molecular distillation to extract the characteristic aroma components and the remaining essential oil components. The characteristic aroma components are linalool, geraniol, and citronellol; S2. 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol were respectively mixed with 0.5 mmol of 7-diethylamino-4-hydroxymethylcoumarin and 0.8 mmol of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, dispersed in 15 mL of dichloromethane, stirred at room temperature for 12 h, and after the reaction was completed, it was rotary evaporated and passed through a column to obtain the carbonate pre-aroma; S3. Preparation method of the glycyrrhizic acid-Mg 2+ -tannic acid system: ① Magnesium chloride was dissolved in water to make a magnesium chloride solution with a concentration of 20 g / L, and then 5 g of glycyrrhizic acid was added to the magnesium chloride solution, and slowly stirred at 55 °C to obtain the glycyrrhizic acid-Mg 2+ solution; ② Tannic acid was added with water to make a tannic acid solution with a concentration of 20 g / L. 50 mL of the tannic acid solution was mixed with 50 mL of the glycyrrhizic acid-Mg 2+ solution and mixed evenly to obtain the glycyrrhizic acid-Mg 2+ -tannic acid solution, which is the glycyrrhizic acid-Mg 2+ -tannic acid system; S4. 20 mL of the carbonate pre-aroma and 20 mL of the remaining essential oil components were mixed evenly with 80 mL of the glycyrrhizic acid-Mg 2+ -tannic acid system, and microemulsified at 30 °C and 7500 r / min for 45 min to obtain the plant essential oil composition B1. Example 7 A preparation method of a plant essential oil composition for soothing emotions, comprising the following steps: S1. Extract the characteristic aroma components and the remaining essential oil components from the plant essential oil composition A2 prepared in Example 2 by molecular distillation. The characteristic aroma components are linalool, geraniol, and citronellol. S2. Mix 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol with 0.5 mmol of 7 - diethylamino - 4 - hydroxymethylcoumarin and 0.8 mmol of 1 - ethyl - (3 - dimethylaminopropyl)carbodiimide hydrochloride respectively, disperse them in 15 mL of dichloromethane, stir at room temperature for 12 h, spin - dry and pass through a column after the reaction to obtain the carbonate - type pre - fragrance precursor. S3. Preparation method of the glycyrrhizic acid - Mg 2+ - tannic acid system: ① Dissolve magnesium chloride in water to make a magnesium chloride solution with a concentration of 20 g / L, then add 5 g of glycyrrhizic acid to the magnesium chloride solution, and slowly stir at 55 °C to obtain the glycyrrhizic acid - Mg 2+ solution; ② Make a 20 g / L tannic acid solution by adding water to tannic acid, mix 50 mL of the tannic acid solution with 50 mL of the glycyrrhizic acid - Mg 2+ solution evenly to prepare the glycyrrhizic acid - Mg 2+ - tannic acid solution, which is the glycyrrhizic acid - Mg 2+ - tannic acid system. S4. Mix 20 mL of the carbonate - type pre - fragrance precursor and 20 mL of the remaining essential oil components with 80 mL of the glycyrrhizic acid - Mg<> 2+ - tannic acid system evenly, and perform micro - emulsification treatment at 30 °C and 7500 r / min for 45 min to obtain the plant essential oil composition B2. Example 8 A preparation method of a plant essential oil composition for relieving mood, comprising the following steps: S1. Extract the characteristic aroma components and the remaining essential oil components from the plant essential oil composition A3 prepared in Example 3 by molecular distillation. The characteristic aroma components are linalool, geraniol, citronellol, and menthol. S2. Mix 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol, 1 mmol of menthol with 0.5 mmol of 7 - diethylamino - 4 - hydroxymethylcoumarin and 0.8 mmol of 1 - ethyl - (З - dimethylaminopropyl)carbodiimide hydrochloride respectively, disperse them in 15 mL of dichloromethane, stir at room temperature for 12 h, spin - dry and pass through a column after the reaction to obtain the carbonate - type pre - fragrance precursor. [[ID=⒛]]S3. Preparation method of the glycyrrhizic acid - Mg 2+ - tannic acid system: ① Dissolve magnesium chloride in water to make a magnesium chloride solution with a concentration of 20 g / L, then add 5 g of glycyrrhizic acid to the magnesium chloride solution, and slowly stir at 55 °C to obtain the glycyrrhizic acid - Mg 2+Solution; ② Prepare a 20 g / L tannic acid solution by adding water to tannic acid. Mix 50 mL of the tannic acid solution with 50 mL of magnesium glycyrrhizinate solution evenly to obtain a magnesium glycyrrhizinate 2+ -tannic acid solution, which is the magnesium glycyrrhizinate 2+ -tannic acid system; 2+ S4. Mix 20 mL of the carbonate precursor of the top note and 20 mL of the remaining essential oil components with 80 mL of the magnesium glycyrrhizinate 2+ -tannic acid system evenly, and perform microemulsification treatment at 30 °C and 7500 r / min for 45 min to obtain the plant essential oil composition B3. Example 9 A method for preparing a plant essential oil composition for soothing emotions, comprising the following steps: S1. Extract the characteristic aroma components and the remaining essential oil components from the plant essential oil composition A4 prepared in Example 4 by molecular distillation. The characteristic aroma components are linalool, geraniol, citronellol, and menthol; S2. Mix 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol, 1 mmol of menthol with 0.5 mmol of 7-diethylamino-4-hydroxymethylcoumarin and 0.8 mmol of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride respectively, disperse them in 15 mL of dichloromethane, stir at room temperature for 12 h, spin-dry and column-chromatograph after the reaction to obtain the carbonate precursor of the top note; S3. Preparation method of the magnesium glycyrrhizinate 2+ -tannic acid system: ① Dissolve magnesium chloride in water to make a magnesium chloride solution with a concentration of 20 g / L, and then add 5 g of glycyrrhizic acid to the magnesium chloride solution, and slowly stir at 55 °C to obtain a magnesium glycyrrhizinate 2+ solution; ② Prepare a 20 g / L tannic acid solution by adding water to tannic acid. Mix 50 mL of the tannic acid solution with 50 mL of the magnesium glycyrrhizinate 2+ solution evenly to obtain a magnesium glycyrrhizinate 2+ -tannic acid solution, which is the magnesium glycyrrhizinate 2+ -tannic acid system; S4. Mix 20 mL of the carbonate precursor of the top note and 20 mL of the remaining essential oil components with 80 mL of the magnesium glycyrrhizinate 2+ -tannic acid system evenly, and perform microemulsification treatment at 30 °C and 7500 r / min for 45 min to obtain the plant essential oil composition B4. Example 10 A method for preparing a plant essential oil composition for soothing emotions, comprising the following steps: S1. The plant essential oil composition A5 prepared in Example 5 is subjected to molecular distillation to extract the characteristic aroma components and the remaining essential oil components. The characteristic aroma components are linalool, geraniol, citronellol, and menthol; S2. 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol, and 1 mmol of menthol are respectively mixed with 0.5 mmol of 7 - diethylamino - 4 - hydroxymethylcoumarin and 0.8 mmol of 1 - ethyl - (3 - dimethylaminopropyl) carbodiimide hydrochloride, dispersed in 15 mL of dichloromethane, stirred at room temperature for 12 h. After the reaction is completed, it is rotary - evaporated and passed through a column to obtain the carbonate - type pre - fragrance body; S3. Preparation method of the glycyrrhizic acid - Mg 2+ - tannic acid system: ① Dissolve magnesium chloride in water to make a magnesium chloride solution with a concentration of 20 g / L, then add 5 g of glycyrrhizic acid to the magnesium chloride solution, and slowly stir at 55 °C to obtain the glycyrrhizic acid - Mg 2+ solution; ② Make a 20 g / L tannic acid solution by adding water to tannic acid. Mix 50 mL of the tannic acid solution with 50 mL of the glycyrrhizic acid - Mg 2+ solution evenly to obtain the glycyrrhizic acid - Mg 2+ - tannic acid solution, which is the glycyrrhizic acid - Mg 2+ - tannic acid system; S4. Mix 20 mL of the carbonate - type pre - fragrance body and 20 mL of the remaining essential oil components with 80 mL of the glycyrrhizic acid - Mg 2+ - tannic acid system evenly, and perform micro - emulsification treatment at 30 °C and 7500 r / min for 45 min to obtain the plant essential oil composition B5. Example 11 A preparation method of a plant essential oil composition for soothing emotions, comprising the following steps: S1. The plant essential oil composition A5 prepared in Example 5 is subjected to molecular distillation to extract the characteristic aroma components and the remaining essential oil components. The characteristic aroma components are linalool, geraniol, citronellol, and menthol; S2. 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol, and 1 mmol of menthol are respectively mixed with 0.5 mmol of 7 - diethylamino - 4 - hydroxymethylcoumarin and 0.8 mmol of 1 - ethyl - (3 - dimethylaminopropyl) carbodiimide hydrochloride, dispersed in 15 mL of dichloromethane, stirred at room temperature for 12 h. After the reaction is completed, it is rotary - evaporated and passed through a column to obtain the carbonate - type pre - fragrance body; S3. Preparation method of the glycyrrhizic acid - Mg 2+ - tannic acid system: ① Dissolve magnesium chloride in water to make a magnesium chloride solution with a concentration of 20 g / L, then add 6 g of glycyrrhizic acid to the magnesium chloride solution, and slowly stir at 55 °C to obtain the glycyrrhizic acid - Mg 2+Solution; ② Prepare a 20 g / L tannic acid solution by adding water to tannic acid. Mix 50 mL of the tannic acid solution with 50 mL of the glycyrrhizic acid-Mg 2+ solution evenly to obtain the glycyrrhizic acid-Mg 2+ -tannic acid solution, which is the glycyrrhizic acid-Mg 2+ -tannic acid system; S4. Mix 20 mL of the carbonate pre-aroma body and 20 mL of the remaining essential oil components with 80 mL of the glycyrrhizic acid-Mg 2+ -tannic acid system evenly, and perform microemulsification treatment at 30 °C and 7500 r / min for 45 min to obtain the plant essential oil composition B6. Example 12 A preparation method of a plant essential oil composition for relieving emotions, comprising the following steps: S1. Extract the characteristic aroma components and the remaining essential oil components from the plant essential oil composition A5 prepared in Example 5 by molecular distillation. The characteristic aroma components are linalool, geraniol, citronellol, and menthol; S2. Mix 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol, 1 mmol of menthol with 0.5 mmol of 7-diethylamino-4-hydroxymethylcoumarin and 0.8 mmol of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride respectively, disperse them in 15 mL of dichloromethane, stir at room temperature for 12 h, spin dry and column chromatograph after the reaction to obtain the carbonate pre-aroma body; S3. Preparation method of the glycyrrhizic acid-Mg 2+ -tannic acid system: ① Dissolve magnesium chloride in water to make a magnesium chloride solution with a concentration of 20 g / L, and then add 5 g of glycyrrhizic acid to the magnesium chloride solution, and slowly stir at 55 °C to obtain the glycyrrhizic acid-Mg 2+ solution; ② Prepare a 20 g / L tannic acid solution by adding water to tannic acid. Mix 50 mL of the tannic acid solution with 50 mL of the glycyrrhizic acid-Mg 2+ solution evenly to obtain the glycyrrhizic acid-Mg 2+ -tannic acid solution, which is the glycyrrhizic acid-Mg 2+ -tannic acid system; S4. Mix 20 mL of the carbonate pre-aroma body and 30 mL of the remaining essential oil components with 80 mL of the glycyrrhizic acid-Mg 2+ -tannic acid system evenly, and perform microemulsification treatment at 30 °C and 7500 r / min for 45 min to obtain the plant essential oil composition B7. Comparative Example 9 The difference between this comparative example and Example 10 is that the glycyrrhizic acid-Mg 2+ -tannic acid system is replaced with a glycyrrhizic acid system. A preparation method of a plant essential oil composition for relieving emotions, comprising the following steps: S1. The plant essential oil composition A5 prepared in Example 5 was subjected to molecular distillation to extract the characteristic aroma components and the remaining essential oil components. The characteristic aroma components were linalool, geraniol, citronellol, and menthol. S2. 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol, 1 mmol of menthol were respectively mixed with 0.5 mmol of 7 - diethylamino - 4 - hydroxymethylcoumarin and 0.8 mmol of 1 - ethyl - (3 - dimethylaminopropyl) carbodiimide hydrochloride, dispersed in 15 mL of dichloromethane, stirred at room temperature for 12 h. After the reaction ended, it was rotary - evaporated and passed through a column to obtain the carbonate - type pre - fragrance body. S4. 20 mL of the carbonate - type pre - fragrance body and 20 mL of the remaining essential oil components were mixed evenly with 80 mL of the glycyrrhizic acid system, and micro - emulsified at 30 °C and 7500 r / min for 45 min to prepare the plant essential oil composition C1. Comparative Example 10 The difference between this comparative example and Comparative Example 10 is that micro - emulsification treatment is not used. A preparation method of a plant essential oil composition for soothing emotions, comprising the following steps: S1. The plant essential oil composition A5 prepared in Example 5 was subjected to molecular distillation to extract the characteristic aroma components and the remaining essential oil components. The characteristic aroma components were linalool, geraniol, citronellol, and menthol. S2. 1 mmol of linalool, 1 mmol of geraniol, 1 mmol of citronellol, 1 mmol of menthol were respectively mixed with 0.5 mmol of 7 - diethylamino - 4 - hydroxymethylcoumarin and 0.8 mmol of 1 - ethyl - (3 - dimethylaminopropyl) carbodiimide hydrochloride, dispersed in 15 mL of dichloromethane, stirred at room temperature for 12 h. After the reaction ended, it was rotary - evaporated and passed through a column to obtain the carbonate - type pre - fragrance body. S3. Preparation method of the glycyrrhizic acid - Mg 2+ - tannic acid system: ① Magnesium chloride was dissolved in water to make a magnesium chloride solution with a concentration of 20 g / L, and then 5 g of glycyrrhizic acid was added to the magnesium chloride solution, and slowly stirred at 55 °C to obtain the glycyrrhizic acid - Mg 2+ solution; ② Tannic acid was added with water to make a 20 g / L tannic acid solution. 50 mL of the tannic acid solution was mixed evenly with 50 mL of the glycyrrhizic acid - Mg 2+ solution to prepare the glycyrrhizic acid - Mg 2+ - tannic acid solution, which is the glycyrrhizic acid - Mg 2+ - tannic acid system; S4. 20 mL of the carbonate - type pre - fragrance body and 20 mL of the remaining essential oil components were mixed evenly with 80 mL of the glycyrrhizic acid - Mg 2+ - tannic acid system to prepare the plant essential oil composition C2. Physical and chemical indexes (1) Average particle size Take an appropriate amount of the sample and place it in a 50 mL beaker. Add an appropriate amount of water to dilute it 100 times and mix evenly for standby. Take 1.5 mL of the diluted solution and put it into the particle size cup, and use a laser nanometer particle size analyzer to detect and analyze the particle size. (2) Centrifugal stability Take an appropriate amount of the plant essential oil composition and centrifuge it at 10000 r / min for 20 min. Observe whether there is stratification or turbidity, and analyze the stability of the plant essential oil composition. Table 1 Evaluation of the average particle size and centrifugal stability of Examples 6 - 12 and Comparative Examples 9 - 11 Average particle size (nm) Evaluation of centrifugal stability Example 6 38.59 Clear, transparent, no stratification, no turbidity Example 7 42.36 Clear, transparent, no stratification, no turbidity Example 8 47.15 Clear, transparent, no stratification, no turbidity Example 9 51.47 Clear, transparent, no stratification, no turbidity Example 10 63.84 Clear, transparent, no stratification, no turbidity Example 11 66.35 Clear, transparent, no stratification, no turbidity Example 12 75.29 Clear, transparent, no stratification, no turbidity Comparative Example 9 102.25 Clear, transparent, no stratification, slightly turbid Comparative Example 10 123.66 Clear, transparent, no stratification, turbid As can be seen from Table 1, the average particle size of the plant essential oil compositions prepared in Examples 6 - 12 is less than 100 nm, and their product form is a colorless, clear and transparent liquid, and they have good centrifugal stability. While the particle sizes of the plant essential oil compositions prepared in Comparative Example 9 and Comparative Example 10 are both higher than 100 nm, and their centrifugal stability is lower than that of the examples, showing some suspension or even turbidity. It shows that the Mg 2+ - tannic acid system interacts with the self - assembled hydrogel of glycyrrhizic acid, promotes the construction of the network structure in the glycyrrhizic acid gel, can increase the number of active cross - link points between glycyrrhizic acid nanofibers, improve the binding sites of the characteristic aroma precursors, make the loaded components tightly connected, and thus endow the plant essential oil composition with good stability. (3) Evaluation of the responsive release effect of the plant essential oil composition Divide Examples 10, Comparative Example 9 and Comparative Example 1 into two groups according to equal molar amounts. One group is placed in an opaque sealed glass bottle, and the other group is placed in a transparent sealed glass bottle; at the same time, an equal molar amount of linalool monomer is placed in a transparent sealed glass container as a control; use solid - phase microextraction combined with GC - MS to test the aroma release amounts at 0 h, 2 h, 4 h, 8 h, 12 h, and 24 h respectively; Solid - phase microextraction (SPME) parameters: the extraction head diameter is 75 μm, the coating layer is carboxyl / polydimethylsiloxane (CAR / PDMS). Add an equal molar mass of the sample to 20 mL headspace vials respectively, and add an internal standard (1 μL, 200 mg / mL 2 - nonanol); place the headspace vials in a water bath at 60 °C, equilibrate for 5 min, extract for 30 min, and desorb for 5 min; GC - MS parameters: the chromatographic column is HP - 5 type (30 m×250 μm×0.25 μm), the carrier gas is helium, the flow rate is 2.0 mL / min, the initial temperature of the column oven is 60 °C, hold for 5 min, increase to 140 °C at a rate of 10 °C / min, then increase to 240 °C at a rate of 40 °C / min, and hold for 5 min. Table 2 Cumulative release amount of aroma of Example 10, Comparative Example 9 and Comparative Example 10 As can be seen from Table 2, the plant essential oil compositions prepared in Example 10, Comparative Example 9 and Comparative Example 10 have light responsiveness, and the release time of Example 10 is longer than that of Comparative Example 9 and Comparative Example 10, indicating that the light responsiveness of the characteristic aroma precursor can enable precise control release of the efficacy aroma components of the plant essential oil composition, extend the aroma duration, and achieve precise and controllable mood regulation efficacy. (4) Brain imaging technology The mechanism of the plant essential oil composition prepared in Example 10 to play a soothing mood role was analyzed by brain imaging technology, as follows: ① The brain imaging test was carried out in a well-ventilated laboratory. Before the test started, all subjects were clearly informed of the detailed process of the experiment and ensured that their participation was voluntary. The subjects needed to visit 2 times, once for collecting EEG signals and the other for collecting fNIRS signals; during the experiment, the subjects were required to sit on an ergonomically designed chair in a comfortable position. Before the formal signal collection started, the subjects needed to first fill in the Profile of Mood States (POMS); after completing the questionnaire, the EEG or fNIRS signal collection officially started. The test included a 30s rest period and a 60s aroma-smelling period. The aroma was transmitted to a distance of about 5 - 7 cm from the subject's face at a flow rate of 5 L / min through a gas pump device. The subjects were required to press the green button on the SAGA synchronization module at the moment they smelled the aroma, record the event and use it as the signal for the start of the aroma-smelling period. After the signal collection ended, the subjects needed to fill in the POMS scale again; ② EEG data were collected using the TMSI SAGA EEG device (Twente Medical Systems International BV, the Netherlands). A 64-channel EEG cap arranged according to the international 10-20 system was used to record EEG signals at a sampling rate of 500 Hz. The impedance of each EEG electrode was maintained below 5 kΩ. The Artinis OxyMon near-infrared brain functional imaging device was installed on the head of the test subject. Sixteen optodes (8 emitters and 8 receivers) covered the frontal cortex (the distance between optodes was 30 mm), and a total of 20 channels were obtained. According to the 10 / 20 international system, the center of the middle optode group was placed approximately at FPz, and the optical signal data changes in the brain were monitored in real time at a sampling rate of 10 Hz. EEG data were preprocessed and feature-extracted using MATLAB and the EEGLAB toolbox. Among them, re-referencing used average reference, and the filter bandwidth was set to 0.1 - 70 Hz. To avoid power frequency interference of the current, a 50 Hz notch filter was used. After window preview, independent component analysis was used to remove electrooculogram artifacts. ③ The multitaper fast Fourier transform (MTMFFT) method was used to calculate the power spectral density (PSD) of EEG signals. The area under the curve (AUC) of the PSD corresponding to the δ (1 - 4 Hz), θ (4 - 8 Hz), α (8 - 13 Hz), β (13 - 30 Hz), and γ (30 - 70 Hz) bands in different brain regions and the AUC of the PSD of each channel during the resting and aroma-smelling phases were calculated. Subsequently, in SPSS, after removing outliers using box plots, independent sample t-tests were performed on the average AUC of channels in different brain regions and the AUC of different bands under the frontal lobe. To further identify and localize the source of EEG signals during aroma perception, the LORETA software was used to estimate the distribution of electrical signal sources in the brain. Through independent sample t-tests, significant differences from the resting state were observed within 0 - 0.4 s of smelling the aroma. fNIRS data were preprocessed and feature-extracted using MATLAB and the NIRS-SPM and homer2 toolboxes. The preprocessing included signal amplification and filtering, motion artifact correction, optical density change conversion, and baseline correction. Subsequently, using SPSS software, independent sample t-tests were performed to statistically analyze the changes in the mean concentration of oxyhemoglobin under resting and aroma-smelling conditions. by Figure 3It can be seen that, compared with the resting state, the aroma-smelling state (where the subject smells the plant essential oil composition prepared in Example 10) significantly reduces the levels of tension, fatigue, and fluster in an individual, while significantly enhancing vitality (p < 0.05), but shows no significant changes in anger, depression, and self-dimension, indicating that the aroma can effectively relieve some negative emotions and enhance positive emotions. By quantifying the subjective emotional experience of the individual, the short-form POMS results provide a reference standard and verification basis for the subsequent analysis of EEG data, enhancing the interpretability of the data and the accuracy of emotional efficacy verification. It can be seen that Figure 4 in the aroma-smelling state, the power spectral density of the frontal lobe is significantly higher than that in the resting state (p < 0.05), while no significant differences are shown in other brain regions (left temporal lobe, right temporal lobe, parietal lobe, occipital lobe) between the two states; the activity of the prefrontal lobe is closely related to olfactory activity. In addition, as a brain region closely related to emotion regulation and cognitive function, the enhanced activity of the frontal lobe is related to the improvement of positive emotions or the enhancement of attention in the process of smelling the aroma, indicating that the aroma of the plant essential oil composition prepared in Example 10 has a positive regulatory effect on emotions and cognitive functions. It can be seen that Figure 5 in the aroma-smelling state, the AUC of the θ wave is significantly higher than that in the resting state (p < 0.05), while no significant differences are shown in other bands (δ, α, β, γ), and the θ wave is usually closely related to deep meditation, the initial stage of sleep, deep introspection, and quiet physical states, emotions, and thought processes. Therefore, the enhancement of the θ wave in the aroma-smelling state indicates that the aroma of the plant essential oil composition prepared in Example 10 induces a more relaxed or focused mental state. It can be seen that Figure 6It can be seen that within 0 - 0.4 s in the aroma - smelling state, compared with the resting state, there are significant differences in the distribution of signal sources (p < 0.05). A total of 34 voxels show significant differences between the two states, and these voxels are mainly concentrated in the anterior cingulate gyrus in the limbic lobe, the subcallosal gyrus in the frontal lobe, the medial frontal gyrus, and the gyrus rectus area. These brain regions have clear functional associations with emotional changes. Among them, ① the processing of olfactory signals is mainly located in the orbitofrontal cortex, and the regions of the orbitofrontal cortex will be activated by pleasant touch, painful touch, taste, smell, and more abstract reinforcers (such as winning or losing money); ② post - traumatic stress disorder is related to the reduced reactivity of the rostral anterior cingulate cortex and the adjacent ventromedial prefrontal cortex. By measuring the changes in cerebral blood flow when listening to music segments with different degrees of harmony using positron emission tomography, it is found that as the degree of music disharmony increases, the activities of the right parahippocampal gyrus and the precuneus region increase; while as the music becomes more harmonious, the activities of the orbitofrontal cortex, the subcallosal cingulate gyrus, and the frontal pole cortex increase. This finding indicates that when positive emotions are perceived, the activities of the orbitofrontal cortex and the subcallosal cingulate gyrus increase, which is consistent with the results of brain source activation when smelling the sample, indirectly verifying that smelling the plant essential oil composition can induce positive emotions in the subjects. In addition, meditation can bring a sense of relaxation, and significant signal enhancement is observed in regions such as the dorsolateral prefrontal cortex, the parietal cortex, the hippocampus, the temporal lobe, the anterior part of the anterior cingulate cortex, the basal ganglia, and the precentral gyrus and postcentral gyrus through fMRI; the source - localization signal changes induced by meditation are similar to those of the fragrance base, and the soothing effect of the plant essential oil composition can be verified together with the results of the subjective scale. It is known from Figure 7 It can be seen that in the aroma - smelling state, the aroma stimulation of the plant essential oil composition prepared in Example 10 results in a significantly lower concentration of oxyhemoglobin in the right prefrontal cortex than that of the control group smelling air in the resting state (p < 0.05). There is no significant difference in the concentration of oxyhemoglobin in the left prefrontal cortex. Smelling the plant essential oil composition produces specific psychological and physiological effects by affecting brain activity and the autonomic nervous system. The significant decrease in the concentration of oxyhemoglobin in the right prefrontal cortex may be related to the activation of the parasympathetic nervous system. Specifically, the decrease in the concentration of oxyhemoglobin in the right prefrontal cortex may reflect a decrease in brain activity or a reduction in energy metabolism, which is consistent with the activation of the parasympathetic nervous system. This phenomenon reflects the regulatory effect of the plant essential oil composition on the autonomic nervous system and brain activity, thus producing a relaxing and comfortable emotional effect. In summary, the plant essential oil composition can significantly enhance the power spectral density (PSD) of the prefrontal cortex and promote theta wave (4 - 8 Hz) activity, indicating that it can induce an electroencephalogram pattern similar to that in a state of deep meditation or relaxation; further source localization reveals that the fragrance base activates brain regions closely related to emotional regulation, such as the anterior cingulate gyrus of the limbic lobe, the inferior gyrus of the frontal corpus callosum, and the medial frontal gyrus. These regions play a key role in positive emotion processing and cognitive control. At the same time, fNIRS data show that the fragrance base stimulation significantly reduces the oxyhemoglobin concentration in the right prefrontal cortex, which is related to the activation of the parasympathetic nervous system and may promote physiological relaxation by inhibiting physiological stress responses (such as reducing blood pressure and heart rate). Overall, the compound fragrance base realizes emotional soothing at the neurophysiological level by synergistically activating the emotion regulation brain network (such as the anterior cingulate gyrus and medial frontal lobe), enhancing prefrontal theta wave activity, and inhibiting the metabolic activity of the right prefrontal lobe to activate the parasympathetic nerve. The above description is only a preferred embodiment of the present invention and does not impose any formal limitations on the present invention. Any person skilled in the art can make many possible changes and modifications to the technical solution of the present invention, or modify it into an equivalent embodiment with equivalent changes, without departing from the spirit and technical solution of the present invention. Therefore, any simple modification, equivalent replacement, equivalent change, and modification made to the above embodiments based on the technical essence of the present invention without departing from the technical solution of the present invention still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A plant essential oil composition for relieving emotions, characterized in that, The plant essential oil composition is composed of two or more of the following essential oils: lavender oil, geranium oil, lemon oil, peppermint oil, rosemary oil, Rosa banksiae Ait. essential oil, osmanthus essential oil, and Roman chamomile essential oil.

2. The preparation method of a plant essential oil composition for relieving emotions according to claim 1, characterized in that, It includes the following steps: Mix various essential oil components evenly to prepare plant essential oil composition A.

3. The preparation method of a plant essential oil composition for relieving emotions according to claim 2, characterized in that, It also includes the following steps: Step 1. The characteristic aroma components and the remaining essential oil components are extracted from plant essential oil composition A by molecular distillation, and the characteristic aroma components are subjected to precursor treatment to obtain pre-aromas. Step 2. Use glycyrrhizic acid-Mg 2+ - Embed the pre-aroma body and the remaining essential oil components with the tannic acid system, and then perform microemulsification treatment to obtain the plant essential oil composition B.

4. The preparation method of a plant essential oil composition for relieving emotions according to claim 3, characterized in that: The characteristic aroma components in step 1 include linalool, geraniol, citronellol, and menthol.

5. The preparation method of a plant essential oil composition for relieving emotions according to claim 3, characterized in that: The precursor treatment in step 1 is to generate ester pre-aromas of the characteristic aroma components and carbonate substances.

6. The preparation method of a plant essential oil composition for soothing emotions according to claim 3, characterized in that, The glycyrrhizic acid-Mg in step 2 2+ - Preparation method of tannic acid system: ① Dissolve magnesium chloride in water to make a magnesium chloride solution with a concentration of 15-20 g / L, and then add 3-6 wt% glycyrrhizic acid to the magnesium chloride solution, and slowly stir at 50-70 °C to obtain glycyrrhizic acid-Mg 2+ solution; ② Make a tannic acid solution by adding water to tannic acid, and mix it evenly with the glycyrrhizic acid-Mg 2+ solution to prepare a glycyrrhizic acid-Mg 2+ - tannic acid solution, which is the glycyrrhizic acid-Mg 2+ - tannic acid system.

7. The preparation method of a plant essential oil composition for relieving emotions according to claim 6, characterized in that: The concentration of tannic acid in ② is 15-35 g / L; the volume ratio of the tannic acid solution to the glycyrrhizic acid-Mg 2+ solution is (1-3):(1-4).

8. The preparation method of a plant essential oil composition for relieving emotions according to claim 3, characterized in that: The conditions for the microemulsification treatment in step 2 are: treatment temperature 25-35°C, stirring speed 6000-9000 r / min, and treatment time 40-60 min.

9. Use of the plant essential oil composition according to claim 1 or the plant essential oil composition prepared by the method according to any one of claims 2-8 in the preparation of a product for relieving emotions.

10. The application according to claim 9, wherein In the aroma-smelling state, the AUC of the theta wave in the frontal lobe of the brain of the product for relieving emotions is significantly higher than that in the resting state.

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