Traditional Chinese medicine compound composition capable of promoting blood circulation, tonifying spleen and clearing damp

Abstract

The invention discloses a traditional Chinese medicine compound composition capable of activating blood, tonifying spleen and clearing damp, and belongs to the technical field of traditional Chinese medicine compound composition preparation. The traditional Chinese medicine compound composition capable of activating blood, invigorating spleen and clearing damp is prepared from salvia miltiorrhiza, astragalus membranaceus, poria cocos, rhizoma alismatis, rhizoma atractylodis, cortex phellodendri, radix achyranthis bidentatae, rheum officinale, ligusticum wallichii, eucommia ulmoides, mangnolia officinalis, radix paeoniae alba, pulp of dogwood fruit, dendrobe, honey-fried licorice root, ginger, fructus amomi, radix polygonati officinalis, platycodon grandiflorum, moutan bark, mustard, rhizoma polygonati, purple perilla, agastache rugosus, cinnamon and a compound antioxidant. The traditional Chinese medicine compound composition prepared by the method has the effects of activating blood circulation to dissipate stasis, tonifying spleen and eliminating dampness.

Description

Technical Field

[0001] The present invention belongs to the technical field of the preparation of traditional Chinese medicine compound compositions, and particularly relates to a traditional Chinese medicine compound composition capable of promoting blood circulation, strengthening the spleen and removing dampness. Background Art

[0002] Since ancient times, traditional Chinese medicine has made great contributions to the reproduction and health of the Chinese nation with its unique theoretical system and practical experience. As the core part of traditional Chinese medicine, the history of traditional Chinese medicine compound can be traced back to "Huangdi Neijing" in the pre-Qin period, and through the continuous practice and summary of medical experts in past dynasties, a unique theoretical system has gradually formed, covering the prevention and treatment of various diseases.

[0003] With the development of modern society, people's life rhythm has accelerated, work pressure has increased, and combined with bad eating habits, the situations of blood stasis and spleen and stomach dysfunction have increased day by day. These pathological changes not only affect people's physical health, but may also lead to the occurrence of a series of chronic diseases. Therefore, finding a safe and effective traditional Chinese medicine compound composition with the main treatment principle of promoting blood circulation and strengthening the spleen has become an important topic in the field of traditional Chinese medicine.

[0004] The advantage of traditional Chinese medicine compound composition lies in its multi-component and multi-target action mechanism. Compared with chemical drugs with a single component, traditional Chinese medicine compound can more comprehensively regulate the metabolic function of the human body and reduce the occurrence of adverse reactions. For example, some heat-clearing drugs in traditional Chinese medicine compounds can promote the excretion of uric acid; dampness-draining drugs can reduce the synthesis of uric acid; and tonic drugs can improve the physical condition of patients and enhance the disease resistance of the body. This characteristic of multi-component synergistic action makes traditional Chinese medicine compound have unique advantages in the treatment of promoting blood circulation and strengthening the spleen. Summary of the Invention

[0005] The purpose of the present invention is to provide a traditional Chinese medicine compound composition capable of promoting blood circulation, strengthening the spleen and removing dampness, so as to solve the technical problem that the functions of promoting blood circulation to remove blood stasis and strengthening the spleen and removing dampness of the traditional Chinese medicine compound composition in the prior art are not good.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0007] The invention provides a traditional Chinese medicine compound composition capable of promoting blood circulation, strengthening the spleen and removing dampness. The traditional Chinese medicine compound composition comprises the following components in parts by weight: 15-20 parts of salvia miltiorrhiza, 15-20 parts of astragalus, 15-20 parts of poria, 12-15 parts of oriental rhizome, 12-15 parts of atractylodes, 9-12 parts of phellodendron, 12-15 parts of cyperus rotundus, 6-9 parts of rhubarb, 9-12 parts of ligusticum chuanxiong, 12-15 parts of eucommia bark, 9-12 parts of magnolia bark, 12-15 parts of white peony root, 12-15 parts of cornus fruit, 12-15 parts of dendrobium, 6-9 parts of roasted liquorice, 3-6 parts of ginger, 3-6 parts of amomum villosum, 6-9 parts of polygonatum, 6-9 parts of platycodon, 6-9 parts of moutan bark, 6-9 parts of mustard, 6-9 parts of polygonatum, 6-9 parts of perilla, 6-9 parts of patchouli, 6-9 parts of cinnamon bark, and 2-6 parts of complex antioxidants.

[0008] Preferably, the invention comprises the following ingredients in parts by weight: 18 parts of salvia miltiorrhiza, 16 parts of astragalus, 16 parts of poria, 14 parts of oriental zedoaria, 14 parts of atractylodes, 10 parts of phellodendron, 14 parts of cyperus, 8 parts of rhubarb, 10 parts of ligusticum, 14 parts of ligusticum, 10 parts of magnolia bark, 13 parts of white peony root, 14 parts of cornus fruit, 14 parts of dendrobium, 8 parts of roasted licorice, 5 parts of ginger, 5 parts of amomum villosum, 8 parts of polygonatum, 8 parts of platycodon, 7 parts of peony bark, 7 parts of mustard, 7 parts of polygonatum, 8 parts of perilla, 7 parts of patchouli, 8 parts of cinnamon, and 4 parts of complex antioxidants.

[0009] Preferably, the method for preparing the composite antioxidant comprises the following steps:

[0010] S1: Add curcumin to a container, slowly add nitric acid solution, stir and mix evenly, then add sodium selenite, stir and add barium chloride, heat in a water bath to react, cool after the reaction, adjust the pH, add sodium sulfate, centrifuge, and take the supernatant for dialysis to obtain a composite selenide;

[0011] S2: Add anhydrous ethanol to a container, then add the complex selenium compound, stir and mix to obtain solution 1, add soy protein isolate to a container filled with deionized water, stir and dissolve to obtain solution 2, then add β-cyclodextrin to a container filled with deionized water, stir and dissolve to obtain solution 3, mix solution 2 and solution 3, heat and magnetically stir, then add solution 1 and Tween-80, adjust the pH, magnetically stir, freeze-dry, grind, and obtain a complex antioxidant.

[0012] In the above process, sodium selenite is hydrolyzed into selenious acid under the catalysis of nitric acid and barium chloride. Subsequently, selenious acid can undergo esterification reaction with the hydroxyl groups contained in curcumin to obtain a complex selenide. Subsequently, soy protein isolate and β-cyclodextrin are used as wall materials, and the complex selenide is used as the core material to prepare a complex antioxidant.

[0013] Preferably, in S1, the dosage ratio of curcumin, nitric acid solution, sodium selenite and barium chloride is (0.45 - 0.55) g : (50 - 55) mL : (0.35 - 0.45) g : (0.002 - 0.0028) g, the volume fraction of the nitric acid solution is 0.5 vt%, the reaction temperature for water bath heating is 60 - 65 °C, the reaction time is 10 - 12 h, sodium hydroxide is added to adjust the pH to 6.8 - 7.2, and the molecular weight cut-off of the dialysis bag during dialysis is 8000 - 10000 Da.

[0014] Preferably, in S2, the dosage ratio of the composite selenium compound, soy protein isolate and β-cyclodextrin is (4 - 6) g : (6 - 7) g : (22 - 22.5) g, the temperature for heating with magnetic stirring is 50 - 55 °C, the magnetic stirring time is 10 - 15 min, the pH is adjusted to 4 - 4.1, and the magnetic stirring time is 20 - 25 min.

[0015] Preferably, a preparation method of a traditional Chinese medicine compound composition capable of activating blood circulation, strengthening the spleen and removing dampness includes the following steps:

[0016] Step 1: Mix the formula amounts of salvia miltiorrhiza, astragalus membranaceus, poria cocos, alisma orientale, atractylodes lancea, phellodendron amurense, achyranthes bidentata, rheum officinale, ligusticum chuanxiong, eucommia ulmoides, magnolia officinalis, white peony root, cornel fruit, dendrobium officinale, roasted licorice root, ginger, amomum villosum, polygonatum odoratum, platycodon grandiflorum, moutan cortex, brassica juncea, polygonatum sibiricum, perilla frutescens, pogostemon cablin, cinnamon, to obtain a mixed traditional Chinese medicine material, add water for decoction, filter, and concentrate to obtain a decoction;

[0017] Step 2: Mix the decoction with a composite antioxidant to obtain a traditional Chinese medicine compound composition capable of activating blood circulation and strengthening the spleen.

[0018] Preferably, the dosage ratio of the mixed traditional Chinese medicine material to water is (100 - 200) g : (800 - 2000) mL, and the decoction is carried out 3 - 6 times.

[0019] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are as follows:

[0020] 1. The present invention uses salvia miltiorrhiza and astragalus membranaceus as the monarch drugs, poria cocos, alisma orientale and atractylodes lancea as the minister drugs, phellodendron amurense, achyranthes bidentata, rheum officinale, ligusticum chuanxiong, eucommia ulmoides, magnolia officinalis, white peony root, cornel fruit and dendrobium officinale as the assistant drugs, and is combined with traditional Chinese medicines such as roasted licorice root, ginger and amomum villosum, and finally a composite antioxidant is added to prepare a traditional Chinese medicine compound composition with the effects of promoting blood circulation to remove blood stasis and strengthening the spleen and removing dampness.

[0021] 2. The present invention prepares a composite antioxidant with curcumin, sodium selenite, soy protein isolate and β-cyclodextrin, and adding it to the traditional Chinese medicine compound composition can effectively improve its antioxidant performance. Detailed implementation manners

[0022] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] Example 1: This example discloses a method for preparing a composite antioxidant, comprising the following steps:

[0024] S1: 0.55 g of curcumin was added to a container, and 50 mL of a 0.5% nitric acid solution was slowly added and stirred to mix evenly. Then, 0.4 g of sodium selenite was added, and after stirring, 0.0022 g of barium chloride was added. The mixture was heated in a water bath at 65°C for 12 h. After the reaction was completed, the mixture was cooled, and sodium hydroxide was added to adjust the pH to 7. Sodium sulfate was added and the mixture was centrifuged. The supernatant was dialyzed. The molecular weight cutoff of the dialysis bag during the dialysis process was 10,000 Da to obtain a composite selenide.

[0025] S2: Add 50 mL of anhydrous ethanol to a container, then add 4 g of a complex selenium compound, stir and mix to obtain solution 1, add 6 g of soy protein isolate to a container filled with 50 mL of deionized water, stir and dissolve to obtain solution 2, then add 22 g of β-cyclodextrin to a container filled with 100 mL of deionized water, stir and dissolve to obtain solution 3, mix solution 2 and solution 3, heat at 50°C and magnetically stir for 10 minutes, then add solution 1 and 0.1 g of Tween-80, adjust the pH to 4, magnetically stir for 25 minutes, freeze-dry, grind, and obtain a complex antioxidant.

[0026] Example 2: This example discloses a Chinese herbal compound composition that can invigorate blood circulation, strengthen the spleen and eliminate dampness. It is composed of the following ingredients, by weight: 17.5 parts of Salvia miltiorrhiza, 17.5 parts of Astragalus membranaceus, 17.5 parts of Poria cocos, 13.5 parts of Alisma orientalis, 13.5 parts of Atractylodes macrocephala, 10.5 parts of Phellodendron chinense, 13.5 parts of Achyranthes bidentata, 7.5 parts of Rhubarb, 10.5 parts of Chuanxiong, 13.5 parts of Eucommia bark, 10.5 parts of Magnolia officinalis, 13.5 parts of White Peony Root, 13.5 parts of Cornus officinalis, 13.5 parts of Dendrobium candidum, 7.5 parts of Radix Glycyrrhizae Preparata, 4.5 parts of Ginger, 4.5 parts of Amomum villosum, 7.5 parts of Polygonatum odoratum, 7.5 parts of Platycodon grandiflorum, 7.5 parts of Paeonia lactiflora, 7.5 parts of Mustard seeds, 7.5 parts of Polygonatum sibiricum, 7.5 parts of Perilla frutescens, 7.5 parts of Patchouli, 7.5 parts of cinnamon bark, and 4 parts of complex antioxidants.

[0027] This embodiment discloses a method for preparing a Chinese medicinal compound composition capable of promoting blood circulation, strengthening the spleen and removing dampness, comprising the following steps:

[0028] Step 1: Mix the formulated amounts of Danshen, Astragalus, Poria, Alisma, Atractylodes, Phellodendron, Achyranthes, Rhubarb, Chuanxiong, Eucommia, Magnolia, White Peony, Cornus, Dendrobium, Rhizoma Licorice, Glycyrrhiza, Ginger, Amomum, Polygonatum, Platycodon, Paeonia, Mustard, Polygonatum, Perilla, Patchouli, and Cinnamon to obtain a mixed Chinese medicinal material, add water and decoct for 5 times, with the ratio of the mixed Chinese medicinal material to water being 150 g:1500 mL, filter, concentrate, and obtain a decoction;

[0029] Step 2: mixing the decoction with the compound antioxidant to obtain a Chinese medicinal compound composition capable of promoting blood circulation and strengthening the spleen.

[0030] Example 3: This example discloses a Chinese herbal compound composition that can promote blood circulation, strengthen the spleen and eliminate dampness. It is composed of the following ingredients, by weight: 20 parts of Salvia miltiorrhiza, 20 parts of Astragalus membranaceus, 20 parts of Poria cocos, 12 parts of Alisma orientalis, 12 parts of Atractylodes macrocephala, 12 parts of Phellodendron chinense, 12 parts of Achyranthes bidentata, 7.5 parts of Rhubarb, 12 parts of Chuanxiong, 12 parts of Eucommia bark, 12 parts of Magnolia officinalis, 12 parts of White Peony Root, 12 parts of Cornus officinalis, 12 parts of Dendrobium candidum, 9 parts of Radix Glycyrrhizae, 3 parts of Ginger, 3 parts of Amomum villosum, 9 parts of Polygonatum odoratum, 9 parts of Platycodon grandiflorum, 9 parts of Paeonia lactiflora, 9 parts of Mustard seeds, 9 parts of Polygonatum sibiricum, 6 parts of Perilla frutescens, 6 parts of Patchouli, 6 parts of cinnamon bark, and 4 parts of complex antioxidants.

[0031] This embodiment discloses a method for preparing a Chinese medicinal compound composition capable of promoting blood circulation, strengthening the spleen and removing dampness, comprising the following steps:

[0032] Step 1: Mix the formulated amounts of Danshen, Astragalus, Poria, Alisma, Atractylodes, Phellodendron, Achyranthes, Rhubarb, Chuanxiong, Eucommia, Magnolia, White Peony, Cornus, Dendrobium, Rhizoma Licorice, Glycyrrhiza, Ginger, Amomum, Polygonatum, Platycodon, Paeonia, Mustard, Polygonatum, Perilla, Patchouli, and Cinnamon to obtain a mixed Chinese medicinal material, add water and decoct for 5 times, with the ratio of the mixed Chinese medicinal material to water being 150 g:1500 mL, filter, concentrate, and obtain a decoction;

[0033] Step 2: mixing the decoction with the compound antioxidant to obtain a Chinese medicinal compound composition capable of promoting blood circulation and strengthening the spleen.

[0034] Example 4: This example discloses a Chinese herbal compound composition that can invigorate blood circulation, strengthen the spleen and eliminate dampness. It is composed of the following ingredients, by weight: 15 parts of Salvia miltiorrhiza, 15 parts of Astragalus membranaceus, 15 parts of Poria cocos, 15 parts of Alisma orientalis, 15 parts of Atractylodes macrocephala, 11 parts of Phellodendron chinense, 14 parts of Achyranthes bidentata, 8 parts of Rhubarb, 11 parts of Chuanxiong, 12 parts of Eucommia ulmoides, 11 parts of Magnolia officinalis, 14 parts of White Peony Root, 14 parts of Cornus officinalis, 14 parts of Dendrobium candidum, 8 parts of Radix Glycyrrhizae Preparata, 5 parts of Ginger, 5 parts of Amomum villosum, 8 parts of Polygonatum odoratum, 8 parts of Platycodon grandiflorum, 7 parts of Paeonia suffruticosa, 7 parts of Mustard seeds, 7 parts of Polygonatum sibiricum, 8 parts of Perilla frutescens, 8 parts of Patchouli, 8 parts of cinnamon bark, and 4 parts of complex antioxidants.

[0035] This embodiment discloses a method for preparing a Chinese medicinal compound composition capable of promoting blood circulation, strengthening the spleen and removing dampness, comprising the following steps:

[0036] Step 1: Mix the formulated amounts of Danshen, Astragalus, Poria, Alisma, Atractylodes, Phellodendron, Achyranthes, Rhubarb, Chuanxiong, Eucommia, Magnolia, White Peony, Cornus, Dendrobium, Rhizoma Licorice, Glycyrrhiza, Ginger, Amomum, Polygonatum, Platycodon, Paeonia, Mustard, Polygonatum, Perilla, Patchouli, and Cinnamon to obtain a mixed Chinese medicinal material, add water and decoct for 5 times, with the ratio of the mixed Chinese medicinal material to water being 150 g:1500 mL, filter, concentrate, and obtain a decoction;

[0037] Step 2: mixing the decoction with the compound antioxidant to obtain a Chinese medicinal compound composition capable of promoting blood circulation and strengthening the spleen.

[0038] Example 5: This example discloses a Chinese herbal compound composition that can promote blood circulation, strengthen the spleen and eliminate dampness. It is composed of the following ingredients, by weight: 16 parts of Salvia miltiorrhiza, 18 parts of Astragalus membranaceus, 17 parts of Poria cocos, 12 parts of Alisma orientalis, 13 parts of Atractylodes macrocephala, 9 parts of Phellodendron chinense, 13 parts of Achyranthes bidentata, 6 parts of Rhubarb, 10 parts of Chuanxiong, 13 parts of Eucommia bark, 10 parts of Magnolia officinalis, 14 parts of White Peony Root, 15 parts of Cornus officinalis, 13 parts of Dendrobium candidum, 7 parts of Radix Glycyrrhizae Preparata, 3 parts of Ginger, 4 parts of Amomum villosum, 8 parts of Polygonatum odoratum, 9 parts of Platycodon grandiflorum, 9 parts of Paeonia lactiflora, 8 parts of Mustard seeds, 8 parts of Polygonatum sibiricum, 7 parts of Perilla frutescens, 8 parts of Patchouli, 8 parts of cinnamon bark, and 4 parts of complex antioxidants.

[0039] This embodiment discloses a method for preparing a Chinese medicinal compound composition capable of promoting blood circulation, strengthening the spleen and removing dampness, comprising the following steps:

[0040] Step 1: Mix the formulated amounts of Danshen, Astragalus, Poria, Alisma, Atractylodes, Phellodendron, Achyranthes, Rhubarb, Chuanxiong, Eucommia, Magnolia, White Peony, Cornus, Dendrobium, Rhizoma Licorice, Glycyrrhiza, Ginger, Amomum, Polygonatum, Platycodon, Paeonia, Mustard, Polygonatum, Perilla, Patchouli, and Cinnamon to obtain a mixed Chinese medicinal material, add water and decoct for 5 times, with the ratio of the mixed Chinese medicinal material to water being 150 g:1500 mL, filter, concentrate, and obtain a decoction;

[0041] Step 2: mixing the decoction with the compound antioxidant to obtain a Chinese medicinal compound composition capable of promoting blood circulation and strengthening the spleen.

[0042] Example 6: This example discloses a Chinese herbal compound composition that can promote blood circulation, strengthen the spleen and eliminate dampness. It is composed of the following ingredients, by weight: 19 parts of Salvia miltiorrhiza, 19 parts of Astragalus membranaceus, 19 parts of Poria cocos, 15 parts of Alisma orientalis, 14 parts of Atractylodes macrocephala, 10 parts of Phellodendron chinense, 14 parts of Achyranthes bidentata, 7 parts of Rhubarb, 10 parts of Chuanxiong, 13 parts of Eucommia bark, 11 parts of Magnolia officinalis, 13 parts of White Peony Root, 14 parts of Cornus officinalis, 15 parts of Dendrobium candidum, 8 parts of Radix Glycyrrhizae, 5 parts of ginger, 4 parts of Amomum villosum, 7 parts of Polygonatum odoratum, 8 parts of Platycodon grandiflorum, 9 parts of Paeonia suffruticosa, 7 parts of Mustard seeds, 8 parts of Polygonatum sibiricum, 9 parts of Perilla frutescens, 9 parts of Patchouli, 9 parts of cinnamon bark, and 4 parts of complex antioxidants.

[0043] This embodiment discloses a preparation method of a traditional Chinese medicine compound composition for activating blood circulation, strengthening the spleen and removing dampness, comprising the following steps:

[0044] Step 1: Mix the following traditional Chinese medicines in the formula amount: Salvia miltiorrhiza, Astragalus membranaceus, Poria cocos, Alisma orientale, Atractylodes lancea, Phellodendron amurense, Achyranthes bidentata, Rheum palmatum, Ligusticum chuanxiong, Eucommia ulmoides, Magnolia officinalis, Paeonia lactiflora, Cornus officinalis, Dendrobium officinale, Honey-fried Licorice Root, Ginger, Amomum villosum, Polygonatum odoratum, Platycodon grandiflorum, Paeonia suffruticosa, Brassica juncea, Polygonatum sibiricum, Perilla frutescens, Pogostemon cablin, Cinnamomum cassia to obtain a mixed traditional Chinese medicine material, decoct it with water 5 times, the dosage ratio of the mixed traditional Chinese medicine material to water is 150 g: 1500 mL, filter and concentrate to obtain a decoction;

[0045] Step 2: Mix the decoction with a compound antioxidant to obtain a traditional Chinese medicine compound composition for activating blood circulation and strengthening the spleen.

[0046] Comparative Example 1: Compared with Example 2, in the process of preparing the traditional Chinese medicine compound composition for activating blood circulation and strengthening the spleen in Comparative Example 1, Poria cocos and Alisma orientale are not added, and other conditions remain unchanged.

[0047] Comparative Example 2: Compared with Example 2, in the process of preparing the traditional Chinese medicine compound composition for activating blood circulation and strengthening the spleen in Comparative Example 2, Atractylodes lancea, Polygonatum odoratum, Platycodon grandiflorum, Paeonia suffruticosa, Brassica juncea, Polygonatum sibiricum, Perilla frutescens, Pogostemon cablin and Cinnamomum cassia are not added, and other conditions remain unchanged.

[0048] Comparative Example 3: Compared with Example 2, in the process of preparing the traditional Chinese medicine compound composition for activating blood circulation and strengthening the spleen in Comparative Example 3, Phellodendron amurense, Rheum palmatum, Polygonatum odoratum, Platycodon grandiflorum, Paeonia suffruticosa, Brassica juncea, Polygonatum sibiricum, Perilla frutescens, Pogostemon cablin and Cinnamomum cassia are not added, and other conditions remain unchanged.

[0049] Comparative Example 4: Compared with Example 2, in the process of preparing the traditional Chinese medicine compound composition for activating blood circulation and strengthening the spleen in Comparative Example 4, Achyranthes bidentata, Ligusticum chuanxiong, Polygonatum odoratum, Platycodon grandiflorum, Paeonia suffruticosa, Brassica juncea, Polygonatum sibiricum, Perilla frutescens, Pogostemon cablin and Cinnamomum cassia are not added, and other conditions remain unchanged.

[0050] Comparative Example 5: Compared with Example 2, in the process of preparing the traditional Chinese medicine compound composition for activating blood circulation and strengthening the spleen in Comparative Example 5, Paeonia lactiflora, Cornus officinalis, Polygonatum odoratum, Platycodon grandiflorum, Paeonia suffruticosa, Brassica juncea, Polygonatum sibiricum, Perilla frutescens, Pogostemon cablin and Cinnamomum cassia are not added, and other conditions remain unchanged.

[0051] Comparative Example 6: Compared with Example 2, in the process of preparing the traditional Chinese medicine compound composition for activating blood circulation and strengthening the spleen in Comparative Example 6, Honey-fried Licorice Root, Ginger, Polygonatum odoratum, Platycodon grandiflorum, Paeonia suffruticosa, Brassica juncea, Polygonatum sibiricum, Perilla frutescens, Pogostemon cablin and Cinnamomum cassia are not added, and other conditions remain unchanged.

[0052] Comparative Example 7: Compared with Example 2, in the process of preparing the Chinese herbal compound composition for promoting blood circulation and strengthening the spleen in Comparative Example 7, no Amomum villosum, Polygonatum odoratum, Platycodon grandiflorum, Paeonia suffruticosa, Mustard seed, Polygonatum sibiricum, Perilla frutescens, Patchouli and Cinnamon bark were added, and other conditions remained unchanged.

[0053] 1. Activate blood circulation and remove blood stasis

[0054] Animals: 30 healthy rabbits, half male and half female, weighing 2-3 kg;

[0055] The traditional Chinese medicine compound composition prepared according to Example 2 was diluted with distilled water to concentrations of 50 mg / mL, 120 mg / mL, and 200 mg / mL, which were low, medium, and high concentrations, respectively;

[0056] The dissolution rate of different concentrations of the traditional Chinese medicine compound composition on rabbit thrombus in vitro was determined. The test method was as follows: 25% urethane was injected into the ear vein of the rabbit at an injection volume of 4 mL / kg. The animals were anesthetized, the right common carotid artery was isolated, 10 mL of blood was collected, and 300 μL of 0.5% fibrinogen solution, 200 μL of 50 g / L calcium chloride solution, and 1.0×10 5 The thrombi were placed in a 20 cm siliconized rubber tube containing 300 μL of U / L thrombin solution; the ends of the tube were closed with water stop clamps and placed in a 37°C constant temperature water bath for 20 min to form an experimental thrombus. The thrombi were cut into 1 cm small segments, weighed, and placed in 5 mL penicillin bottles. The cells were randomly divided into a normal saline group, an aspirin positive control group, a low concentration group, a medium concentration group, and a high concentration group. Each group was given 4 mL of the drug and placed in a 37°C constant temperature water bath. The thrombi were removed at 1 h, 2 h, 4 h, 6 h, and 8 h, respectively, and the water was aspirated and weighed. The dissolution rate (%) was calculated as [(M 栓初 -M 栓后 ) / M 栓初 ]×100%;

[0057] The dissolution rate of rabbit blood clots in vitro by the Chinese herbal compound composition of different concentrations was determined. The test method was as follows: 20 mL of blood was collected from the common carotid artery of a rabbit, placed in an empty culture dish, and placed in a constant temperature water bath at 37°C for 15 minutes. After the blood coagulated, it was removed and cut into experimental blood clots of 0.5 cm × 0.5 cm × 0.5 cm. The blood clots were rinsed 3 times with normal saline, dried, weighed, and placed in a vial. 4 mL of the corresponding drug solution was added according to the above grouping decibels, and the blood clots were placed in a constant temperature water bath at 37°C. The blood clots were removed at 24 hours and 48 hours, dried, and weighed. The dissolution rate (%) was calculated as follows: [(M 块初 -M 块后 ) / M 块初 ]×100%;

[0058] Determination of the aggregation experiment of traditional Chinese medicine compound composition with different concentrations on rabbit platelets in vitro: 15 mL of blood was taken from the common carotid artery of rabbits and injected into a centrifuge tube rinsed with sodium citrate. After centrifugation at 1200 rpm for 10 min, the supernatant was taken as platelet-rich plasma (PRP). The remaining blood was centrifuged at 4000 rpm for 10 min, and the supernatant was taken as platelet-poor plasma (PPP). The cell density of PRP was adjusted to 3.0×10 8 cells / mL with PPP. PRP was added to a 96-well plate, 200 μL per well. According to the aforementioned experimental grouping, 5 μL of each drug was administered to each group. The mixture was pipetted and blown for 20 s to mix evenly, and incubated at 37 °C for 5 min. 17 μL of 40 μmol / L ADP was added to each component to induce platelet aggregation. Immediately, it was placed in an enzyme-linked immunosorbent assay (ELISA) reader. At a wavelength of 655 nm, the absorbance A was measured dynamically. It was measured once every 30 s, and shaken intermittently for 20 s until the absorbance A no longer changed. According to the platelet aggregation rate (%) = [(A t0 -A t ) / A t0 ×100%, and according to the platelet aggregation inhibition rate (%) = [(aggregation rate of the normal saline group - aggregation rate of the drug administration group) / aggregation rate of the normal saline group]×100%. The experimental results are shown in Tables 1, 2, and 3 as follows:

[0059] Table 1 Effects of traditional Chinese medicine compound composition on the thrombolysis rate of rabbits in vitro

[0060]

[0061] Table 2 Effects of traditional Chinese medicine compound composition on the dissolution rate of rabbit thrombus in vitro

[0062]

[0063] Table 3 Effects of traditional Chinese medicine compound composition on the aggregation of rabbit platelets in vitro

[0064]

[0065] Thrombus is caused by damage to the vascular intima. Insoluble fibrin, deposited platelets, accumulated white blood cells and red blood cells in the blood deposit at the damaged site, causing partial or complete blockage of blood flow, resulting in tissue blood supply disorders and triggering thrombotic diseases. From the above experimental results, it can be seen that the low-concentration traditional Chinese medicine compound composition has a certain thrombolytic effect on thrombus, while the medium- and high-concentration traditional Chinese medicine compound compositions have a tendency of thrombolysis, indicating that the traditional Chinese medicine compound composition has a thrombolytic effect on thrombus within a certain concentration range; a blood clot is a blood mass formed by the extravasation of blood flowing out due to vascular injury and rupture, and coagulating in the tissue or on the skin surface. The data shows that the traditional Chinese medicine compound composition only has a tendency of thrombolysis on blood clots, indicating that its thrombolytic effect on thrombus is stronger. Platelet aggregation plays an important role in the formation of thrombus. Using the platelet aggregation inducer ADP to induce in vitro aggregation of rabbit platelets, the traditional Chinese medicine compound composition can inhibit ADP-induced platelet aggregation and has a certain anti-platelet aggregation effect.

[0066] II. Invigorating the spleen and removing dampness

[0067] Animals: 40 healthy male SD rats at 4 weeks of age, with a mass of about 90 g;

[0068] Senna leaf aqueous solution: Senna leaves are added to purified water and soaked for 30 min, heated to boiling and then heated for another 10 min, filtered and concentrated to a concentration of 1.2 g / mL, and stored at 4°C;

[0069] Shenling Baizhu powder medicinal liquid: Shenling Baizhu powder is added to purified water and stirred to dissolve, with a concentration of 0.5 g / mL, and stored at 4°C;

[0070] Forty rats were randomly divided into a blank group, a model group, a traditional Chinese medicine compound composition group, and a positive drug Shenling Baizhu Powder group, with 10 rats in each group. They were adaptively fed for 3 days. From the 4th day, the body weights of the rats in each group were recorded, which was the first day of the formal experiment. Since the first day of the formal experiment, except for the blank group, the remaining rats were given intragastric administration of an aqueous extract of Rheum officinale Baill. once a day in the morning, and the administration dose was 10 mL / kg. Except for the blank group, the rats in each group were placed in a water bucket to swim once until exhaustion in the evening. The sign of exhaustion was that the rats no longer struggled to swim after entering the water, the nose tip sank into the water for 5 s, and they could not surface. This continued for 14 days. On the 15th day of the experiment, the model was evaluated, and the rats with successful modeling were selected for drug administration. On the 15th day of the experiment, the traditional Chinese medicine compound composition group was administered at a dose of 2.1 g / kg according to a concentration of 0.21 g / mL, the positive drug Shenling Baizhu Powder group was administered at a dose of 5 g / kg, and the blank group was given intragastric administration of purified water. The administration continued for 14 days. One day after the end of modeling and one day after the end of drug administration, the rats in each group were fasted but allowed to drink water. The abdomen of the rats was gently pressed to stimulate defecation, and the feces were collected. After the fasting ended, the rats were given D-xylose solution, and the urine was collected for 5 h. On the 30th day of the experiment, samples were taken. A fresh carbon powder suspension was prepared, and 2 mL was given to each rat. The total mass of the carbon powder suspension and the syringe was recorded, and the actual mass of the carbon powder administered to each rat was calculated. According to the gastric residue rate = [(total weight of the stomach containing carbon powder - net weight of the washed stomach) / (total mass of 2 mL carbon powder and the syringe - mass of the blank syringe)] × 100%, 15 minutes later, the rats were intraperitoneally injected with 10% chloral hydrate for anesthesia. The abdominal aorta blood was taken by dissection and collected in anticoagulant tubes and non-anticoagulant tubes respectively. The stomach, digestive tract from the pylorus to the cecum, and kidneys were taken, and the carbon powder propulsion length and total small intestine length were measured. After the measurement, the colon was cut, opened along the longitudinal axis of the mesentery, the contents were washed with normal saline, and they were stored separately. The total weight of the stomach was weighed, the contents were washed with normal saline after opening the stomach, and it was weighed again and stored in ice. The abdominal aorta blood was allowed to stand for 30 minutes and then centrifuged at 3500 rpm for 15 minutes to obtain serum and plasma. The rat urine was centrifuged at 3500 rpm for 15 minutes to obtain the supernatant. The serum and plasma of the rats were taken, and the levels of serum SS, plasma MTL, and VIP were detected by ELISA method. The kidneys and colon were added with PBS buffer and homogenized on ice, and the levels of AQP2 in the kidney homogenate and AQP3 in the colon homogenate were detected by ELISA method. The test results are shown in Tables 4, 5, 6, 7, and 8:

[0071] Table 4 Effects of traditional Chinese medicine compound composition on fecal moisture content of rats

[0072] Group Moisture content after modeling / % Moisture content after administration / % Blank group 50.43±6.12 56.78±5.83 Model group 84.67±6.57 88.64±6.33 Traditional Chinese medicine compound composition group 82.34±6.12 60.54±4.67 Positive drug Shenling Baizhu Powder group 83.45±6.34 59.78±5.67

[0073] Table 5 Effects of traditional Chinese medicine compound composition on D-xylose excretion rate of rats

[0074] Group D-xylose excretion rate after modeling / % D-xylose excretion rate after administration / % Blank group 10.43±5.12 10.02±5.11 Model group 4.23±2.57 3.96±1.21 Traditional Chinese medicine compound composition group 2.34±1.02 5.87±2.17 Positive drug Shenling Baizhu Powder group 3.05±1.34 5.78±2.14

[0075] Table 6 Effects of Traditional Chinese Medicine Compound Composition on Gastric Residual Rate and Small Intestine Propulsion Rate in Rats

[0076] Group Gastric residue rate / % Small intestine propulsion rate / % Blank group 23.43±2.78 45.02±4.71 Model group 62.73±7.93 32.65±6.04 Traditional Chinese medicine compound composition group 33.74±5.93 38.94±5.93 Positive drug Shenling Baizhu Powder group 33.12±4.86 40.74±7.12

[0077] Table 7 Effects of Traditional Chinese Medicine Compound Composition on Gastrointestinal Hormone Levels in Rats

[0078] Group <![CDATA[MTL / (pg·mL -1 )]]> <![CDATA[VIP / (pg·mL -1 )]]> <![CDATA[SS / (ng·mL -1 )]]> Blank group 122.12±12.64 29.56±1.45 473.85±54.85 Model group 52.37±11.53 32.88±0.68 612.75±67.93 Traditional Chinese medicine compound composition group 97.44±18.64 31.17±0.11 501.63±68.52 Positive drug Shenling Baizhu Powder group 121.25±18.93 30.36±1.04 482.93±77.35

[0079] Table 8 Effects of Traditional Chinese Medicine Compound Composition on the Protein Expressions of Occludin and Claudin-1 in the Colon of Rats

[0080]

[0081]

[0082] MTL is a polypeptide gastrointestinal hormone that can promote gastrointestinal motility, stimulate pepsin secretion, accelerate the discharge of gastric contents, and promote the recovery of gastric solvent. SS is somatostatin, which can weaken gastrointestinal motility and slow down gastric emptying. VIP is a consistent brain-gut peptide that can directly act on the receptors on gastric smooth muscle, relax smooth muscle and sphincter, inhibit small intestine motility, and weaken gastrointestinal motility. D-xylose is mainly absorbed in the upper part of the jejunum, cannot be utilized by the liver, and most of it is excreted from urine through the kidneys. The excretion rate of xylose in urine can reflect the absorption ability of the small intestine. After the model establishment, the D-xylose excretion rate of rats in each group except the blank group decreased significantly, indicating the successful replication of the model. After drug intervention, the D-xylose excretion rate of rats in the administration group increased significantly, suggesting that its intestinal absorption function was improved; both Occludin and Claudin-1 are tight junction proteins in the intestinal barrier structure, and their functions are to maintain the structure of epithelial cells, control the transport of related ions, and maintain and regulate intestinal permeability. In the experiment, the protein levels of Occludin and Claudin-1 in the colon of rats in the model group decreased, and intestinal permeability increased. After drug intervention, the protein levels of Occludin and Claudin-1 in rats in the traditional Chinese medicine compound group increased. Therefore, the traditional Chinese medicine compound composition can improve intestinal permeability, adjust the body's water and salt balance, and relieve diarrhea symptoms.

[0083] As mentioned above, it is only the preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present invention.

[0084] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific embodiments only. Obviously, according to the content of this specification, many modifications and changes can be made. These embodiments are selected and specifically described in this specification in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can well understand and utilize the present invention. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A traditional Chinese medicine compound composition for promoting blood circulation, strengthening the spleen and removing dampness, characterized in that, By weight parts, it consists of the following components: 15 - 20 parts of Salvia miltiorrhiza, 15 - 20 parts of Astragalus membranaceus, 15 - 20 parts of Poria cocos, 12 - 15 parts of Alisma orientale, 12 - 15 parts of Atractylodes lancea, 9 - 12 parts of Phellodendron amurense, 12 - 15 parts of Achyranthes bidentata, 6 - 9 parts of Rheum palmatum, 9 - 12 parts of Ligusticum chuanxiong, 12 - 15 parts of Eucommia ulmoides, 9 - 12 parts of Magnolia officinalis, 12 - 15 parts of Paeonia lactiflora, 12 - 15 parts of Cornus officinalis, 12 - 15 parts of Dendrobium officinale, 6 - 9 parts of honey - fried licorice root, 3 - 6 parts of ginger, 3 - 6 parts of Amomum villosum, 6 - 9 parts of Polygonatum odoratum, 6 - 9 parts of Platycodon grandiflorum, 6 - 9 parts of Paeonia suffruticosa, 6 - 9 parts of Brassica juncea, 6 - 9 parts of Polygonatum sibiricum, 6 - 9 parts of Perilla frutescens, 6 - 9 parts of Pogostemon cablin, 6 - 9 parts of Cinnamomum cassia, 2 - 6 parts of compound antioxidant.

2. The traditional Chinese medicine compound composition for activating blood circulation, strengthening the spleen and removing dampness according to claim 1, wherein, By weight parts, it consists of the following components: 18 parts of Salvia miltiorrhiza, 16 parts of Astragalus membranaceus, 16 parts of Poria cocos, 14 parts of Alisma orientale, 14 parts of Atractylodes lancea, 10 parts of Phellodendron amurense, 14 parts of Achyranthes bidentata, 8 parts of Rheum palmatum, 10 parts of Ligusticum chuanxiong, 14 parts of Eucommia ulmoides, 10 parts of Magnolia officinalis, 13 parts of Paeonia lactiflora, 14 parts of Cornus officinalis, 14 parts of Dendrobium officinale, 8 parts of honey - fried licorice root, 5 parts of ginger, 5 parts of Amomum villosum, 8 parts of Polygonatum odoratum, 8 parts of Platycodon grandiflorum, 7 parts of Paeonia suffruticosa, 7 parts of Brassica juncea, 7 parts of Polygonatum sibiricum, 8 parts of Perilla frutescens, 7 parts of Pogostemon cablin, 8 parts of Cinnamomum cassia, 4 parts of compound antioxidant.

3. The traditional Chinese medicine compound composition capable of activating blood circulation, strengthening the spleen and eliminating dampness according to claim 1, wherein The preparation method of the compound antioxidant includes the following steps: S1: Add curcumin into a container, slowly add nitric acid solution, stir and mix evenly, then add sodium selenite, stir and then add barium chloride, react by water - bath heating, after the reaction ends, cool, adjust the pH, add sodium sulfate, centrifuge, and take the supernatant for dialysis to obtain a compound selenide; S2: Add anhydrous ethanol into a container, then add the compound selenium compound, stir and mix to obtain Solution 1. Add soy protein isolate into a container filled with deionized water, stir and dissolve to obtain Solution 2. Then add β - cyclodextrin into a container filled with deionized water, stir and dissolve to obtain Solution 3. Mix Solution 2 and Solution 3, heat and stir magnetically, then add Solution 1 and Tween - 80, adjust the pH, stir magnetically, freeze - dry, and grind to obtain the compound antioxidant.

4. The traditional Chinese medicine compound composition capable of activating blood circulation, strengthening the spleen and removing dampness according to claim 3, wherein In S1, the dosage ratio of curcumin, nitric acid solution, sodium selenite and barium chloride is (0.45 - 0.55) g:(50 - 55) mL:(0.35 - 0.45) g:(0.002 - 0.0028) g, the volume fraction of the nitric acid solution is 0.5 vt%, the water - bath heating reaction temperature is 60 - 65 °C, the reaction time is 10 - 12 h, add sodium hydroxide to adjust the pH = 6.8 - 7.2, and the molecular weight cut - off of the dialysis bag during dialysis is 8000 - 10000 Da.

5. The traditional Chinese medicine compound composition capable of activating blood circulation, strengthening the spleen and removing dampness according to claim 3, wherein, In S2, the dosage ratio of the compound selenium compound, soy protein isolate and β - cyclodextrin is (4 - 6) g:( 6. The preparation method of a traditional Chinese medicine compound composition capable of activating blood circulation, strengthening the spleen and removing dampness according to any one of claims 1-5, characterized in that, ​ Step 1: Mix the following traditional Chinese medicines in formula amounts: Salvia miltiorrhiza, Astragalus membranaceus, Poria cocos, Alisma orientale, Atractylodes lancea, Phellodendron amurense, Achyranthes bidentata, Rheum palmatum, Ligusticum chuanxiong, Eucommia ulmoides, Magnolia officinalis, Paeonia lactiflora, Cornus officinalis, Dendrobium officinale, Radix Glycyrrhizae Preparata, Ginger, Amomum villosum, Polygonatum odoratum, Platycodon grandiflorum, Paeonia suffruticosa, Brassica juncea, Polygonatum sibiricum, Perilla frutescens, Pogostemon cablin, Cinnamomum cassia to obtain a mixed traditional Chinese medicine material, add water for decoction, filter, and concentrate to obtain a decoction solution; Step 2: Mix the decoction solution with a compound antioxidant to obtain a traditional Chinese medicine compound composition that can promote blood circulation and strengthen the spleen.

7. The preparation method of a traditional Chinese medicine compound composition capable of promoting blood circulation, strengthening the spleen and removing dampness according to claim 6, characterized in that, The dosage ratio of the mixed traditional Chinese medicine material to water is (100 - 200) g:(800 - 2000) mL, and decoct for 3 - 6 times.

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