Embryo and ovum vitrification freezing carrier device for assisted reproduction

Abstract

The invention relates to the technical field of assisted reproduction, and provides an embryo and ovum vitrification freezing carrier device for assisted reproduction, which comprises a vacuum box and a liquid nitrogen tank fixed in the vacuum box through a support column, the inner side of the liquid nitrogen tank is rotatably connected with a turntable, and the turntable is provided with a plurality of annularly distributed placing holes; a carrier for loading reproductive test tubes is placed in the placing hole, and a plug sealing assembly is arranged at the top of the vacuum box; a dynamic sealing taking and placing mechanism is adopted, a sealing plug assembly is designed, a detachable sealing channel is formed by an overpressure cover, a telescopic rod and a plug head, a jacking assembly is designed, a lifting rod can be driven through a rotary knob, a spring buffering mechanism is combined, accurate jacking and resetting of a test tube are achieved, and zero-cold-loss rapid switching is achieved through rotary positioning and sealing taking and placing of a rotary disc; the whole process is operated in a closed environment, cross contamination is avoided, long-time storage and repeated taking and placing are supported, and stability is higher.

Description

Technical Field

[0001] The present invention relates to the field of assisted reproductive technologies, and specifically, to a vitrification cryopreservation carrier device for embryos and eggs used in assisted reproduction. Background Art

[0002] Assisted reproductive technology, abbreviated as ART, refers to the technology that uses medical assistance means to enable infertile couples to become pregnant, including two major categories: artificial insemination and in vitro fertilization-embryo transfer and its derivative technologies. Currently, in assisted reproductive treatment, under the action of ovulation induction drugs, multiple eggs can be obtained, and multiple embryos can be obtained after in vitro fertilization. However, only 1-3 embryos can be implanted during embryo implantation. To prevent pregnancy failure or for other reasons, the remaining high-quality embryos or eggs are cryopreserved, and the cryopreserved embryos or eggs can be thawed, transplanted, or fertilized in the next cycle, improving the utilization rate of embryos and eggs. Embryos are frozen at a temperature of -196°C, stop developing, and can be stored for several years.

[0003] After retrieval, the patent technology with the publication number CN117944984A discloses a vitrification cryopreservation carrier device for embryos and eggs used in assisted reproduction, including: a housing having a receiving chamber and an opening at the top, and an openable cover is provided on the opening at the top; a tube rack is arranged in the receiving chamber, and a plurality of cell collection tubes are placed on the tube rack; a lifting device is connected to the tube rack, and the lifting device is used to drive the tube rack to move towards the opening at the top of the receiving chamber so that only the upper section of the cell collection tube extends out of the opening at the top. In this device, the tube rack is arranged in the receiving chamber through the lifting device. When taking the cell collection tube, the tube rack is lifted by the lifting device, and only the upper section of the cell collection tube extends out of the opening at the top of the receiving chamber, so that the lower section of the cell collection tube remains in the receiving chamber all the time. Therefore, when selecting the cell collection tube for a long time, the other cell collection tubes on the tube rack will not be heated too high.

[0004] However, the prior art still has the following problems: 1. In terms of cold storage maintenance: only relying on the local sealing of the liquid nitrogen tank, the vacuum box (housing) does not participate in heat preservation; 2. In terms of cold storage maintenance when taking out the sample, the tube rack needs to be lifted, resulting in cold loss; 3. In terms of sample safety, the opening operation is likely to introduce a pollution risk; 4. In terms of environmental adaptability, it is only applicable to short-term storage and retrieval scenarios. Summary of the Invention

[0005] The present invention provides a vitrification cryopreservation carrier device for embryos and eggs used in assisted reproduction, which solves the problems of poor heat preservation, cold loss, and pollution risk in the prior art.

[0006] The technical solution of the present invention is as follows: An embryo and egg vitrification freezing carrier device for assisted reproduction, including a vacuum chamber, a liquid nitrogen tank fixed in the vacuum chamber through support columns. A turntable is rotatably connected to the inner side of the liquid nitrogen tank. A number of annularly distributed placement holes are provided on the turntable, and carriers for loading reproductive test tubes are placed in the placement holes. A sealing component is provided at the top of the vacuum chamber, and a lifting component is provided at the bottom of the vacuum chamber. The sealing component and the lifting component are vertically aligned with one of the carriers. When the test tube in this carrier needs to be taken out, it can be lifted upward by the lifting component and then sealed up and down with the carrier and the sealing component, so as to take out the test tube in a closed environment. A rotating mechanism capable of driving the turntable to rotate to adjust the position of the carrier is provided at the top of the vacuum chamber.

[0007] Preferably, the carrier includes a mounting head. A convex ring capable of supporting the mounting head in the placement hole is fixed on the outer wall of the mounting head. A conduit is fixed at the bottom of the mounting head and passes downward through the placement hole.

[0008] Preferably, in the non-force state, the head of the reproductive test tube is recessed in the mounting head and below the upper surface of the turntable, and a part of the body of the reproductive test tube extends out from the bottom opening of the conduit. Markings related to sample information can be set on the surface of the head of the reproductive test tube.

[0009] Preferably, the sealing component includes a through-tube. The through-tube is fixedly embedded in the top of the vacuum chamber. A gland for closing the through-tube is threadedly connected to the outer side of the top of the through-tube. A guide tube is fixed to the inner side of the bottom of the gland. A telescopic rod is slidably connected to the inner side of the bottom of the guide tube. A plug is fixed at the bottom of the telescopic rod. The plug is elastically connected to the guide tube through a first spring.

[0010] Preferably, the lifting component includes a lifting rod. A guide groove slidably engaged with the liquid nitrogen tank is provided on the lifting rod. The bottom of the lifting rod penetrates downward through the vacuum chamber. A knob sleeved on the lifting rod is rotatably connected to the bottom of the vacuum chamber. An external thread threadedly connected to the knob is provided on the outer side of the lifting rod. A bottom plug is slidably connected to the top end of the lifting rod. A pressing ring is fixed to the outer side of the bottom plug. An annular protrusion is fixed to the outer side of the top of the lifting rod. The pressing ring is elastically connected to the annular protrusion through a second spring.

[0011] Preferably, during the rotation of the turntable, the head of the test tube presses against the plug, which can cause the plug to contract upward against the elastic force of the first spring, and the bottom of the test tube presses against the bottom plug, which can cause the bottom plug to contract downward against the elastic force of the second spring.

[0012] Preferably, the elastic stiffness of the second spring is greater than that of the first spring, so that during the rotation of the turntable, the reproductive test tube in the carrier is lifted upward as a whole, so that the head of the reproductive test tube is higher than the upper surface of the turntable.

[0013] Preferably, a first window is provided on the vacuum chamber at the same height as the head of the reproductive test tube when it is lifted, and a second window corresponding to the position of the first window is provided on the liquid nitrogen tank.

[0014] Preferably, the rotating mechanism includes a main shaft that penetrates through the vacuum chamber and the liquid nitrogen tank and is rotatably connected to the vacuum chamber and the liquid nitrogen tank. A handwheel is fixed to the upper end of the main shaft, and a main gear is fixed to the lower end of the main shaft.

[0015] Preferably, the rotating mechanism further includes a plurality of auxiliary shaft rods arranged circumferentially around the main shaft, and the auxiliary shaft rods are all rotatably connected to the liquid nitrogen tank. A sub-gear meshing with the main gear is fixed to the lower end of the auxiliary shaft rod, and a toothed ring meshing with the sub-gear is provided at the center of the turntable.

[0016] The beneficial effects of the present invention are as follows: The present invention adopts a vacuum-liquid nitrogen composite heat preservation system. The heat of the external environment is isolated by the vacuum chamber to maintain the low-temperature environment of the liquid nitrogen tank. The turntable inside the liquid nitrogen tank is directly immersed in liquid nitrogen to ensure ultra-low temperature preservation of the samples throughout the process. The installation head + conduit structure of the modular carrier design allows the head of the test tube to be embedded in the installation head (recessed on the surface of the turntable), and the tube body extends through the conduit to avoid shaking. A marking area is provided on the surface of the test tube head for easy identification of sample information; The present invention adopts a dynamic sealing picking and placing mechanism. By designing a plugging assembly, a detachable sealing channel is formed by an overpressure cover, a telescopic rod, and a plug head. By designing a jacking assembly, the lifting rod can be driven by a knob, combined with a spring buffer mechanism, to achieve precise jacking and resetting of the test tube. Using turntable rotation positioning + sealed picking and placing, "zero cold loss" rapid switching is realized, and the operation is carried out in a fully enclosed environment to avoid cross-contamination, support long-term preservation and multiple picking and placing, and has higher stability; The present invention adopts a rotary drive system. Through the gear and toothed ring transmission, a multi-stage transmission structure of the main shaft-main gear-sub-gear-toothed ring is used to achieve the smooth rotation of the turntable, and the operation of the handwheel simplifies the operation process of manually switching samples and improves efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0018] Figure 1 is a schematic diagram of the overall structure of an embryo and egg vitrification freezing carrier device for assisted reproduction proposed by the present invention; Figure 2 is a schematic cross-sectional structure diagram of an embryo and egg vitrification freezing carrier device for assisted reproduction proposed by the present invention; Figure 3 is a schematic diagram of a partial cross-sectional structure of the liquid nitrogen tank proposed by the present invention; Figure 4 Structural schematic diagram of the vehicle, plugging component and lifting component proposed by the present invention; Figure 5 Structural schematic diagram before assembly of the test tube and the vehicle proposed by the present invention; Figure 6 Structural schematic diagram after assembly of the test tube and the vehicle proposed by the present invention; Figure 7 Structural schematic diagram of the lifting component proposed by the present invention; Figure 8 Half-sectional structural schematic diagram of the plugging component proposed by the present invention; In the figure: 1, vacuum chamber; 11, first window; 2, liquid nitrogen tank; 21, second window; 3, support column; 4, turntable; 41, placement hole; 42, toothed ring; 5, rotating mechanism; 51, main shaft; 52, handwheel; 53, main gear; 54, auxiliary shaft rod; 55, auxiliary gear; 6, vehicle; 61, mounting head; 62, convex ring; 63, conduit; 7, plugging component; 71, through pipe; 72, gland; 73, guide pipe; 74, telescopic rod; 75, plug; 76, first spring; 8, lifting component; 81, lifting rod; 82, external thread; 83, knob; 84, bottom plug; 85, pressing ring; 86, annular protrusion; 87, second spring; 88, guide groove. Detailed implementation manners

[0019] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

[0020] Please refer to Figure 1 and Figure 2, the present invention provides a technical solution: an embryo and egg vitrification freezing carrier device for assisted reproduction, including a vacuum chamber 1, a liquid nitrogen tank 2 fixed in the vacuum chamber 1 through a support column 3. A turntable 4 is rotatably connected to the inner side of the liquid nitrogen tank 2. A number of annularly distributed placement holes 41 are provided on the turntable 4, and a carrier 6 for loading reproductive test tubes is placed in the placement holes 41. The reproductive test tubes containing different samples are placed on the turntable 4 in the liquid nitrogen tank 2 through the carrier 6. A gland 72 seals the through-tube 71 to make both the vacuum chamber 1 and the liquid nitrogen tank 2 airtight. The test tubes are cryopreserved by the liquid nitrogen in the liquid nitrogen tank 2, and the inner cavity of the vacuum chamber 1 outside the liquid nitrogen tank 2 is in a vacuum environment to reduce the loss of cold. The technical point of the present invention is that a sealing component 7 is provided at the top of the vacuum chamber 1, and a lifting component 8 is provided at the bottom of the vacuum chamber 1. The sealing component 7 and the lifting component 8 are vertically aligned with one of the carriers 6. When the test tube in this carrier 6 needs to be taken out, it can be lifted upward by the lifting component 8 and sealed up and down with the carrier 6 and the sealing component 7, so as to take out the test tube in a closed environment. A rotating mechanism 5 capable of driving the turntable 4 to rotate to adjust the position of the carrier 6 is provided at the top of the vacuum chamber 1.

[0021] Please refer to Figure 4 and Figure 5 , the carrier 6 includes a mounting head 61. A convex ring 62 capable of supporting the mounting head 61 in the placement hole 41 is fixed on the outer wall of the mounting head 61. A conduit 63 is fixed to the bottom of the mounting head 61 and passes downward through the placement hole 41. In the non-loaded state, the head of the reproductive test tube is recessed in the mounting head 61 and below the upper surface of the turntable 4, and a part of the body of the reproductive test tube extends out from the bottom opening of the conduit 63. Marks related to sample information can be set on the surface of the head of the reproductive test tube.

[0022] Please refer to Figure 4 and Figure 8 , the sealing component 7 includes a through-tube 71. The through-tube 71 is fixedly embedded in the top of the vacuum chamber 1. A gland 72 for sealing the through-tube 71 is threadedly connected to the outer side of the top of the through-tube 71. A guide tube 73 is fixed to the inner side of the bottom of the gland 72. A telescopic rod 74 is slidably connected to the inner side of the bottom of the guide tube 73. A plug 75 is fixed to the bottom of the telescopic rod 74. The plug 75 is elastically connected to the guide tube 73 through a first spring 76.

[0023] Please refer to Figure 4 and Figure 7, the lifting assembly 8 includes a lifting rod 81. A guiding groove 88 which is in sliding fit with the liquid nitrogen tank 2 is formed in the lifting rod 81. The bottom of the lifting rod 81 penetrates downward through the vacuum chamber 1. A knob 83 sleeved outside the lifting rod 81 is rotatably connected to the bottom of the vacuum chamber 1. An external thread 82 which is in threaded connection with the knob 83 is arranged on the outer side of the lifting rod 81. A bottom plug 84 is slidably connected to the top end of the lifting rod 81. A pressing ring 85 is fixed to the outer side of the bottom plug 84. An annular protrusion 86 is fixed to the outer side of the top of the lifting rod 81. The pressing ring 85 is elastically connected to the annular protrusion 86 through a second spring 87. During the rotation of the turntable 4, the head of the test tube presses the plug 75, enabling the plug 75 to tend to contract upward against the elastic force of the first spring 76. And the bottom of the test tube presses the bottom plug 84, enabling the bottom plug 84 to tend to contract downward against the elastic force of the second spring 87. The elastic stiffness of the second spring 87 is greater than that of the first spring 76. During the rotation of the turntable 4, the reproductive test tubes in the carrier 6 are lifted upward as a whole, so that the heads of the reproductive test tubes are higher than the upper surface of the turntable 4. By screwing the knob 83, due to the threaded action between the knob 83 and the external thread 82 outside the lifting rod 81, and simultaneously guided by the sliding of the guiding groove 88, the lifting rod 81 can move upward. Then, through the acting force of the second spring 87, the bottom plug 84 is pushed. The bottom plug 84 holds the test tube and moves upward together with the conduit 63 until the convex ring 62 outside the mounting head 61 abuts against the lower end surface of the through pipe 71. At this time, the carrier 6 stops moving, and a sealed environment can be formed with the upper through pipe 71 and the pressing ring 85 outside the bottom plug 84 below. Then, by screwing the gland 72, the plug 75 is taken out of the through pipe 71. After that, continue to screw the knob 83, and the lifting rod 81 moves upward to lift the test tube out of the through pipe 71, so as to take out the test tube from the liquid nitrogen tank 2 in a sealed environment. Similarly, the test tube can also be put into the liquid nitrogen tank 2 in a sealed environment, thus reducing the loss of liquid nitrogen.

[0024] It should be noted that a first window 11 with the same height as the lifted head of the reproductive test tube is provided on the vacuum chamber 1. A second window 21 corresponding to the position of the first window 11 is provided on the liquid nitrogen tank 2. The head of the lifted reproductive test tube can be observed through the first window 11 and the second window 21, so as to switch the reproductive test tube to between the plugging assembly 7 and the lifting assembly 8 according to the marking.

[0025] Please refer to Figure 2 With Figure 3, the rotating mechanism 5 includes a main shaft 51 which passes through the vacuum chamber 1 and the liquid nitrogen tank 2 and is rotatably connected to the vacuum chamber 1 and the liquid nitrogen tank 2. A handwheel 52 is fixed to the upper end of the main shaft 51, and a main gear 53 is fixed to the lower end of the main shaft 51. The rotating mechanism 5 further includes a plurality of auxiliary shaft rods 54 arranged circumferentially around the main shaft 51, and the auxiliary shaft rods 54 are all rotatably connected to the liquid nitrogen tank 2. A sub-gear 55 meshing with the main gear 53 is fixed to the lower end of the auxiliary shaft rod 54. A tooth ring 42 meshing with the sub-gear 55 is provided at the center of the turntable 4. The heads of the reproductive test tubes with different samples are marked correspondingly according to the sample information stored therein. When it is necessary to take out the reproductive test tube with the corresponding mark, the handwheel 52 can be rotated, and the main shaft 51 drives the main gear 53 to rotate. Through the meshing of the main gear 53 and the sub-gear 55 and the meshing of the sub-gear 55 and the tooth ring 42, the turntable 4 is driven to rotate in the liquid nitrogen tank 2, thereby switching the position of the carrier 6.

[0026] The working principle and usage process of the present invention are as follows: The reproductive test tubes containing different samples are placed on the turntable 4 in the liquid nitrogen tank 2 through the carrier 6. The gland 72 seals the through-tube 71 so that both the vacuum chamber 1 and the liquid nitrogen tank 2 are in a sealed state. The test tubes are cryopreserved by the liquid nitrogen in the liquid nitrogen tank 2, and the inner cavity of the vacuum chamber 1 outside the liquid nitrogen tank 2 is in a vacuum environment to reduce the loss of cold quantity; The heads of the reproductive test tubes with different samples are marked correspondingly according to the sample information stored therein. When it is necessary to take out the reproductive test tube with the corresponding mark, the handwheel 52 can be rotated, and the main shaft 51 drives the main gear 53 to rotate. Through the meshing of the main gear 53 and the sub-gear 55 and the meshing of the sub-gear 55 and the tooth ring 42, the turntable 4 is driven to rotate in the liquid nitrogen tank 2, thereby switching the position of the carrier 6. In the non-loaded state, the head of the reproductive test tube is recessed in the mounting head 61 and is below the upper surface of the turntable 4, and a part of the body of the reproductive test tube extends out from the bottom opening of the conduit 63. During the rotation of the turntable 4, the head of the test tube presses against the plug 75, which can make the plug 75 tend to contract upward against the elastic force of the first spring 76, and the bottom of the test tube presses against the bottom plug 84, which can make the bottom plug 84 tend to contract downward against the elastic force of the second spring 87. Since the elastic stiffness of the second spring 87 is greater than that of the first spring 76, during the rotation of the turntable 4, the reproductive test tube in the carrier 6 is lifted upward as a whole, so that the head of the reproductive test tube is higher than the upper surface of the turntable 4. A first window 11 with the same height as the lifted head of the reproductive test tube is provided on the vacuum chamber 1, and a second window 21 corresponding to the position of the first window 11 is provided on the liquid nitrogen tank 2. The lifted head of the reproductive test tube can be observed through the first window 11 and the second window 21, so that the reproductive test tube can be switched between the sealing assembly 7 and the lifting assembly 8 according to the mark; Then, by screwing the knob 83, the threaded action between the knob 83 and the external thread 82 outside the lifting rod 81, and under the sliding guidance of the guiding groove 88, the lifting rod 81 can move upward. Furthermore, under the action of the second spring 87, the bottom plug 84 is pushed, and the test tube and the conduit 63 are lifted upward together by the bottom plug 84 until the convex ring 62 outside the mounting head 61 abuts against the lower end surface of the through tube 71. At this time, the carrier 6 stops moving and can form a sealed environment with the upper through tube 71 and the pressing ring 85 outside the lower bottom plug 84. Then, by screwing the gland 72, the plug head 75 is removed from the through tube 71. After that, by continuing to screw the knob 83, the lifting rod 81 moves upward and can lift the test tube out of the through tube 71, so as to take out the test tube from the liquid nitrogen tank 2 in a sealed environment. Similarly, the test tube can also be placed into the liquid nitrogen tank 2 in a sealed environment, thereby reducing the loss of liquid nitrogen.

[0027] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An embryo and egg vitrification freezing carrier device for assisted reproduction, comprising a vacuum chamber (1) and a liquid nitrogen tank (2) fixed in the vacuum chamber (1) through a support column (3), characterized in that, A turntable (4) is rotatably connected to the inner side of the liquid nitrogen tank (2). A number of annularly distributed placement holes (41) are formed in the turntable (4), and a carrier (6) for loading reproductive test tubes is placed in the placement holes (41). A plugging assembly (7) is provided at the top of the vacuum box (1), and a jacking assembly (8) is provided at the bottom of the vacuum box (1). The plugging assembly (7) and the jacking assembly (8) are vertically aligned with one of the carriers (6). When the test tube in this carrier (6) needs to be taken out, it can be jacked up by the jacking assembly (8) and then sealed with the carrier (6) and the plugging assembly (7) up and down to take out the test tube in a closed environment. A rotating mechanism (5) capable of driving the turntable (4) to rotate to adjust the position of the carrier (6) is provided at the top of the vacuum box (1).

2. An auxiliary reproductive embryo and egg vitrification freezing carrier device according to claim 1, characterized in that, The carrier (6) includes a mounting head (61). A convex ring (62) capable of supporting the mounting head (61) in the placement hole (41) is fixed on the outer wall of the mounting head (61). A conduit (63) is fixed to the bottom of the mounting head (61) and passes downward through the placement hole (41).

3. An embryo and egg vitrification cryopreservation carrier device for assisted reproduction according to claim 2, characterized in that, In the non-loaded state, the head of the reproductive test tube is recessed in the mounting head (61) and is below the upper surface of the turntable (4), and a part of the body of the reproductive test tube extends out from the bottom opening of the conduit (63). Markings related to sample information can be set on the surface of the head of the reproductive test tube.

4. An embryo and egg vitrification freezing carrier device for assisted reproduction according to claim 1, characterized in that, The plugging assembly (7) includes a through-tube (71). The through-tube (71) is fixedly embedded in the top of the vacuum box (1). A gland (72) for closing the through-tube (71) is threadedly connected to the outer side of the top of the through-tube (71). A guide tube (73) is fixed to the inner side of the bottom of the gland (72). A telescopic rod (74) is slidably connected to the inner side of the bottom of the guide tube (73). A plug (75) is fixed to the bottom of the telescopic rod (74). The plug (75) is elastically connected to the guide tube (73) through a first spring (76).

5. An embryo and egg vitrification freezing carrier device for assisted reproduction according to claim 4, characterized in that, The jacking assembly (8) includes a lifting rod (81). A guide groove (88) slidably engaged with the liquid nitrogen tank (2) is formed in the lifting rod (81). The bottom of the lifting rod (81) extends downward through the vacuum box (1). A knob (83) sleeved on the lifting rod (81) is rotatably connected to the bottom of the vacuum box (1). An external thread (82) threadedly connected to the knob (83) is provided on the outer side of the lifting rod (81). A bottom plug (84) is slidably connected to the top end of the lifting rod (81). A pressing ring (85) is fixed to the outer side of the bottom plug (84). An annular protrusion (86) is fixed to the outer side of the top of the lifting rod (81). The pressing ring (85) is elastically connected to the annular protrusion (86) through a second spring (87).

6. An embryo and egg vitrification freezing carrier device for assisted reproduction according to claim 5, characterized in that, During the rotation of the turntable (4), the head of the test tube presses against the plug (75), which can cause the plug (75) to tend to contract upward against the elastic force of the first spring (76), and the bottom of the test tube presses against the bottom plug (84), which can cause the bottom plug (84) to tend to contract downward against the elastic force of the second spring (87).

7. An embryo and egg vitrification freezing carrier device for assisted reproduction according to claim 6, characterized in that, The elastic stiffness of the second spring (87) is greater than that of the first spring (76), so that during the rotation of the turntable (4), the whole reproductive test tube in the carrier (6) is lifted upward, making the head of the reproductive test tube higher than the upper surface of the turntable (4).

8. An embryo and egg vitrification cryopreservation carrier device for assisted reproduction according to claim 7, characterized in that, A first window (11) with the same height as the lifted head of the reproductive test tube is provided on the vacuum chamber (1), and a second window (21) corresponding to the position of the first window (11) is provided on the liquid nitrogen tank (2).

9. An embryo and egg vitrification freezing carrier device for assisted reproduction according to claim 1, characterized in that, The rotating mechanism (5) includes a main shaft (51) which passes through the vacuum chamber (1) and the liquid nitrogen tank (2) and is rotatably connected to the vacuum chamber (1) and the liquid nitrogen tank (2). A handwheel (52) is fixed to the upper end of the main shaft (51), and a main gear (53) is fixed to the lower end of the main shaft (51).

10. An embryo and egg vitrification cryopreservation carrier device for assisted reproduction according to claim 9, characterized in that, The rotating mechanism (5) further includes a plurality of auxiliary shaft rods (54) arranged circumferentially around the main shaft (51), and the auxiliary shaft rods (54) are all rotatably connected to the liquid nitrogen tank (2). A secondary gear (55) meshing with the main gear (53) is fixed to the lower end of the auxiliary shaft rod (54), and a toothed ring (42) meshing with the secondary gear (55) is provided at the center of the turntable (4).

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