Fertilizer composition containing bacillus velezensis

Abstract

The invention belongs to the technical field of fertilizers, and particularly relates to a fertilizer composition containing bacillus velezensis. The invention discloses a fertilizer composition containing bacillus velezensis, the core component of the fertilizer composition is composed of a fermentation broth of bacillus velezensis CGMCC No.14384 and calcium lignosulphonate, the mass ratio of the fermentation broth of the bacillus velezensis CGMCC No.14384 with the bacterial concentration of 108cfu / mL to the calcium lignosulphonate is 0.5-20: 1-15, and the fertilizer composition has a synergistic effect on plant growth promotion. Field test results show that the fertilizer containing the composition of the fermentation liquor of the bacillus velezensis CGMCC No.14384 and the calcium lignosulphonate has obvious saline-alkali reducing effect and plant growth promoting effect, and can be applied to saline-alkali soil improvement and plant growth promoting.

Description

Technical Field

[0001] The present invention belongs to the technical field of fertilizers, and particularly relates to a fertilizer composition containing Bacillus velezensis. Background Art

[0002] Soil salinization seriously affects crop growth and yield. The high salt content and unsuitable pH value in saline-alkali soil inhibit the root development of crops, damage the soil structure, reduce soil fertility, and thus affect the yield and quality of crops. To address this challenge, the agricultural field has been searching for effective solutions, and bio-fertilizers have received extensive attention due to their environmental protection and sustainable characteristics.

[0003] Bacillus velezensis is a Gram-positive bacterium that is heat-resistant, acid-resistant, and alkali-resistant, and is widely distributed in natural environments such as soil, water, and air. It has extremely strong adaptability and vitality, can form spores under adverse environments, and exhibits rich metabolic pathways and powerful synthesis capabilities. Bacillus velezensis can produce a variety of industrially valuable enzymes and metabolites, and these substances have broad application potential in the agricultural field.

[0004] In recent years, Bacillus velezensis has received extensive attention in plant disease prevention and control and promoting plant growth. It can induce the resistance response of plants by activating various defense enzymes in plants, such as phenylalanine ammonia-lyase, peroxidase, etc., and enhance the resistance of plants to pathogenic bacteria. In addition, Bacillus velezensis can secrete a variety of bioactive substances, including cell wall degrading enzymes, lipopeptide antibiotics, etc., which can effectively inhibit a variety of plant pathogenic bacteria, such as Phytophthora capsici, Fusarium solani, etc., to achieve effective prevention and control of plant diseases. In promoting crop growth, Bacillus velezensis also performs excellently.

[0005] However, there are few reports on the reduction of soil salinity by Bacillus velezensis. It is of great significance to find components that can be compatible with Bacillus velezensis and apply them to the promotion of the growth of plants in saline-alkali land and the improvement of saline-alkali soil. Summary of the Invention

[0006] To solve the above technical problems, the present invention selects the strain Bacillus velezensis CGMCC No.14384 with a growth-promoting effect, studies the components that can produce a synergistic effect when combined with it, and mixes and applies them to the promotion of the growth of plants in saline-alkali land and the improvement of saline-alkali soil.

[0007] On the one hand, the present invention provides a fertilizer composition containing Bacillus velezensis, and the core components of the fertilizer composition are composed of a Bacillus velezensis fermentation broth and calcium lignosulfonate; the preservation number of the Bacillus velezensis is CGMCC No.14384.

[0008] Furthermore, in the fertilizer composition containing Bacillus velezensis, the mass ratio of the Bacillus velezensis fermentation broth to calcium lignosulfonate is 0.5 - 20:1 - 15, and the concentration of Bacillus velezensis in the Bacillus velezensis fermentation broth is 10 8 cfu / mL.

[0009] Furthermore, in the fertilizer composition containing Bacillus velezensis, the mass ratio of the Bacillus velezensis fermentation broth to calcium lignosulfonate is 5:5.

[0010] On the other hand, the present invention also provides a fertilizer containing the fertilizer composition containing Bacillus velezensis.

[0011] Furthermore, the fertilizer contains fulvic acid.

[0012] On yet another aspect, the present invention also provides the application of the fertilizer in promoting plant growth. Applying the fertilizer during the plant growth cycle can promote plant growth.

[0013] Furthermore, in the above application, the plant is a solanaceous plant or a malvaceous plant.

[0014] Furthermore, in the above application, the solanaceous plant is tomato, and the malvaceous plant is cotton.

[0015] Furthermore, in the above application, the application method of the fertilizer is drip irrigation, the application rate of the fertilizer is 2 L / mu / time, and the number of times of applying the fertilizer is 2 times during the plant growth period.

[0016] Furthermore, the application of the fertilizer in improving the soil environment, the improvement of the soil environment is to reduce the soil salinity, and the reduction of the soil salinity is to reduce the soil EC value.

[0017] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0018] By screening existing strains, the present invention selects Bacillus velezensis CGMCC No. 14384, studies components that can be compatible with the fermentation broth of Bacillus velezensis CGMCC No. 14384 and can produce synergistic effects, and finds that the mixture of the Bacillus velezensis fermentation broth (10 8 cfu / mL) and calcium lignosulfonate has a better effect on promoting the growth of tomatoes than single agents within the mass ratio range of 0.5 - 20:1 - 15, indicating that the mixture of the two has a synergistic effect on promoting the growth of tomatoes, and when the mass ratio of the mixture of the Bacillus velezensis fermentation broth (10 8 cfu / mL) and calcium lignosulfonate is 5:5, the synergistic effect of the two is the best.

[0019] Field test results show that applying only the fertilizer containing the fermentation broth of Bacillus velezensis CGMCC No.14384 and calcium lignosulfonate can significantly reduce the EC value of the soil in the cotton planting area. However, applying the contrast agents 1 and 2 containing other Bacillus velezensis and calcium lignosulfonate did not show the effect of reducing the EC value of the soil in the planting area, indicating that the fertilizer composition containing the fermentation broth of Bacillus velezensis CGMCC No.14384 and calcium lignosulfonate has the effect of reducing soil salinity. In particular, after applying the fertilizer with the fermentation broth of Bacillus velezensis CGMCC No.14384 and calcium lignosulfonate mixed in a ratio of 5:5, the soil EC value decreased from 2.80 to 1.75, and the soil salinity reduction effect was the best. In addition, the field test results show that applying the fertilizer containing the fermentation broth of Bacillus velezensis CGMCC No.14384 and calcium lignosulfonate composition has a significant growth-promoting effect on cotton.

[0020] The fertilizer provided by the present invention containing the fermentation broth of Bacillus velezensis CGMCC No.14384 and calcium lignosulfonate composition has a significant effect of reducing soil salinity and promoting plant growth, and can be applied to the improvement of saline-alkali soil and plant growth promotion. Detailed implementation mode

[0021] Next, the technical solutions of the present invention will be described in conjunction with the embodiments. However, the present invention is not limited to the following embodiments.

[0022] In order to enable those skilled in the art to better understand and implement the technical solutions of the present invention, the present invention will be further described below in conjunction with specific embodiments, but the specific embodiments cited are not intended to limit the present invention.

[0023] In the following embodiments, the experimental methods and detection methods are all conventional methods unless otherwise specified; the reagents and materials can be purchased on the market unless otherwise specified.

[0024] Example 1

[0025] This example is for the preparation of the fermentation broth of Bacillus velezensis.

[0026] Bacillus velezensis CGMCC No.14384 stored at -20°C was activated on an NA plate for 24 hours. A single colony was picked and inoculated into an NB liquid medium, and cultured on a shaker at 28°C and 180 rpm for 24 hours. The fermentation broth was collected, centrifuged, and resuspended with distilled water to a concentration of 10 8 cfu / mL for standby.

[0027] Example 2

[0028] This example is a test for the synergistic growth promotion of the fermentation broth of Bacillus velezensis and calcium lignosulfonate.

[0029] Treatment with test reagents: The Bacillus velezensis fermentation broth prepared in Example 1 and calcium lignosulfonate were diluted with water respectively to prepare the mother liquor of the single agent and the mother liquor of the mixed agent with each ratio. Different mass concentration gradients were set for the single agent and the mixed agent with each ratio, and they were reserved for use.

[0030] Test plants: Potted tomatoes.

[0031] The mixed agent and the mother liquor of the single agent with the above different ratios were diluted with clear water at a ratio of 1:100 and reserved for use.

[0032] A clear water group was set as the blank control group (CK group), a single agent group of the test reagent was set, and a mixed agent group, that is, the test group of the composition of the Bacillus velezensis fermentation broth and calcium lignosulfonate, was set.

[0033] The single agent of the Bacillus velezensis fermentation broth and calcium lignosulfonate and the mixed agent were diluted at 1:100 and reserved for use. Select potted tomatoes at the seedling stage with consistent growth conditions. Apply 100 mL of the diluted solution of the single agent and the mixed agent of the Bacillus velezensis fermentation broth and calcium lignosulfonate to each pot. Each treatment was repeated 10 plants. The clear water application group was used as the blank control group (CK group). After 30 days of application, the plant height, stem diameter and root length were statistically analyzed, and the plant height growth rate, stem diameter growth rate and root length growth rate were calculated. The Gowing method was used to evaluate the effect of the mixture of each active ingredient on the growth-promoting performance of tomatoes. The formula is E0 = X + [Y×(100 - X) / 100], where E0 represents the theoretical root length growth rate, theoretical stem diameter growth rate and theoretical plant height growth rate of the composition (A + B) of tomatoes when the dosage is (P + Q). X represents the root length growth rate, stem diameter growth rate and plant height growth rate of the medicament A on tomatoes when the dosage is P, and Y represents the root length growth rate, stem diameter growth rate and plant height growth rate of the medicament B on tomatoes when the dosage is Q; E represents the actual root length growth rate, stem diameter growth rate and plant height growth rate of the tomatoes treated with the composition.

[0034] When E - E0 > 10% is a synergistic effect; when E - E0 < -10% is an antagonistic effect; when the value of E - E0 is between ±10% is an additive effect.

[0035] Root length growth rate (%) = (root length of the test group - root length of the blank control group) / root length of the blank control group × 100

[0036] Stem diameter growth rate (%) = (stem diameter of the test group - stem diameter of the blank control group) / stem diameter of the blank control group × 100

[0037] Plant height growth rate (%) = (plant height of the test group - plant height of the blank control group) / plant height of the blank control group × 100

[0038] The test results of the effects of different treatments on the plant height of tomatoes are shown in Table 1, the test results of the effects of different treatments on the stem diameter of tomatoes are shown in Table 2, and the test results of the effects of different treatments on the root length of tomatoes are shown in Table 3.

[0039] The results in Table 1, Table 2 and Table 3 show that within the range of the mixing mass ratio of Bacillus velezensis fermentation broth to calcium lignosulfonate of 0.5 - 20:1 - 15, the growth-promoting effects of the mixture of Bacillus velezensis fermentation broth and calcium lignosulfonate on the plant height, stem diameter and root length of tomatoes are better than those of single agents, indicating that the mixture of Bacillus velezensis fermentation broth and calcium lignosulfonate has a synergistic effect on the growth promotion of tomatoes. When the mixing mass ratio of Bacillus velezensis fermentation broth and calcium lignosulfonate is in other ranges, the two show an additive effect; when the mixing mass ratio of Bacillus velezensis fermentation broth and calcium lignosulfonate is 5:5, the growth-promoting effect on tomatoes is the most significant and the synergistic effect is the best.

[0040] Table 1 Test results of the effects of different treatments on the plant height of tomatoes

[0041]

[0042]

[0043] Table 2 Test results of the effects of different treatments on the stem diameter of tomatoes

[0044]

[0045]

[0046] Table 3 Test results of the effects of different treatments on the root length of tomatoes

[0047]

[0048]

[0049] Example 2

[0050] This example is a fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate.

[0051] A fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate, including 0.5% of Bacillus velezensis fermentation broth (10 8 cfu / mL), 15% of calcium lignosulfonate, 1% of zinc sulfate, and 10% of fulvic acid by mass percentage. Marked as Fertilizer 1#.

[0052] Example 3

[0053] This example is a fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate.

[0054] A fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate, comprising, by mass percentage, 1% of Bacillus velezensis fermentation broth (10 8 cfu / mL), 10% of calcium lignosulfonate, 2% of zinc sulfate, 12% of fulvic acid, and the balance being water. Marked as Fertilizer 2#.

[0055] Example 4

[0056] This example is a fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate.

[0057] A fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate, comprising, by mass percentage, 5% of Bacillus velezensis fermentation broth (10 8 cfu / mL), 5% of calcium lignosulfonate, 1.5% of zinc sulfate, 5% of fulvic acid, and the balance being water. Marked as Fertilizer 3#.

[0058] Example 5

[0059] This example is a fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate.

[0060] A fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate, comprising, by mass percentage, 10% of Bacillus velezensis fermentation broth (10 8 cfu / mL), 2.5% of calcium lignosulfonate, 2% of zinc sulfate, 7% of fulvic acid, and the balance being water. Marked as Fertilizer 4#.

[0061] Example 6

[0062] This example is a fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate.

[0063] A fertilizer containing a fertilizer composition of Bacillus velezensis fermentation broth and calcium lignosulfonate, comprising, by mass percentage, 20% of Bacillus velezensis fermentation broth (10 8 cfu / mL), 1% of calcium lignosulfonate, 2% of zinc sulfate, 3% of fulvic acid, and the balance being water. Marked as Fertilizer 5#.

[0064] Control 1

[0065] Control 1 is a fertilizer composition containing Bacillus velezensis CICC 10211 fermentation broth.

[0066] Bacillus velezensis CICC 10211 stored at -20°C was activated on NA plates for 24 h, and a single colony was picked and inoculated into NB liquid medium. The culture was placed on a shaker at 28°C and 180 rpm for 24 h, and the fermentation broth was collected, centrifuged, and resuspended in distilled water to a bacterial suspension concentration of 10 8 cfu / mL, and the bacterial suspension of Bacillus Velez CICC 10211 was obtained.

[0067] Weigh 5% of the fermentation broth of Bacillus velezensis CICC 10211 (10 8 cfu / mL), 5% calcium ligninsulfonate, 2% zinc sulfate, 5% fulvic acid, and the balance water. The above ingredients were mixed to produce a fertilizer composition containing the fermentation broth of Bacillus velezensis CICC 10211 and calcium ligninsulfonate, labeled as Comparative Agent 1#.

[0068] Contrast agent 2

[0069] Comparative agent 2 is a fertilizer composition containing Bacillus velezensis CGMCC No. 21866.

[0070] Bacillus velezensis CGMCC No. 21866 stored at -20°C was activated on NA plates for 24 h, and a single colony was picked and inoculated into NB liquid medium. The culture was placed on a shaker at 28°C and 180 rpm for 24 h, and the fermentation broth was collected, centrifuged, and resuspended in distilled water to a bacterial suspension concentration of 10 8 cfu / mL, and the bacterial suspension of Bacillus Velezii CGMCC No.21866 was obtained.

[0071] Weigh 5% of the fermentation broth of Bacillus velezensis CGMCC No.21866 (10 8 cfu / mL), 5% calcium lignin sulfonate, 2% zinc sulfate, 5% fulvic acid, and the balance water. The above materials were mixed to prepare a fertilizer composition containing the fermentation broth of Bacillus velezensis CGMCC No. 21866 and calcium lignin sulfonate, labeled as comparative agent 2#.

[0072] Example 9

[0073] This example is a field fertilizer efficiency test of a fertilizer composition containing a fermentation broth of Bacillus Velez and calcium lignin sulfonate.

[0074] Field test location: The cotton-growing area of Korla, Xinjiang was selected to carry out a field fertilizer efficiency test of a fertilizer composition containing a fermentation liquid of Bacillus Velez and calcium lignin sulfonate.

[0075] Cotton variety tested: Zhaofeng No. 28.

[0076] The test area is a total of 8 mu, evenly divided into 8 test areas, each test area is 1 mu, and 3 replicates are set in each test area. Before cotton planting, basal fertilizer is applied conventionally. 10 days after applying the basal fertilizer, soil samples from 10 test areas are collected to measure the soil EC value, and the soil EC value is recorded. During the cotton seedling stage, fertilizers 1# - 5# of Examples 2 - 6 (Test Group 1 - Test Group 5), Contrast Agent 1# (CK1), Contrast Agent 2# (CK2), and clear water (blank control KC) are applied. The application method is drip irrigation. Drip irrigation is carried out continuously 2 times during the seedling stage. The first drip irrigation is when the cotton plant height reaches 15 cm, and the second drip irrigation is when the cotton plant height reaches 50 cm. The dosage for both drip irrigations is 2 L / mu. During the cotton maturity stage, the number of cotton bolls, single boll weight, and cotton yield data of the cotton are recorded to evaluate the effects of different treatments on cotton growth and its yield. After cotton harvest, soil samples are collected to measure the soil EC value to evaluate the effects of each treatment on the soil ecological structure.

[0077] Table 3 Test Results of the Effects of Different Treatments on Soil EC Value

[0078] Test grouping Soil EC value before application Soil EC value after cotton harvest Change in soil EC value Fertilizer No. 1 2.79 1.95 -0.84 Fertilizer No. 2 2.81 1.92 -0.89 Fertilizer No. 3 2.80 1.75 -1.05 Fertilizer No. 4 2.81 1.95 -0.86 Fertilizer No. 5 2.79 1.97 -0.82 Control agent No. 1 (CK1) 2.75 2.74 -0.01 Control agent No. 2 (CK2) 2.77 2.70 -0.07 Blank control (KC) 2.78 3.45 0.67

[0079] The results of the effects of applying fertilizers with different fertilizer compositions containing Bacillus velezensis fermentation broth and calcium lignosulfonate on the soil ecological structure of the experimental field are shown in Table 3. Before applying the fertilizers with Bacillus velezensis fermentation broth and calcium lignosulfonate and the contrast agent fertilizers, the EC values of the soils in each area of the experimental field were between 2.75 - 2.81, with no significant difference. During the field experiment, the fertilizers with Bacillus velezensis fermentation broth and calcium lignosulfonate and the contrast agent fertilizers were drip-irrigated 2 times. The test results of the soil EC value in the experimental area after cotton harvest showed that compared with the blank control group, the EC values decreased after applying fertilizers 1# - 5#. After applying Contrast Agent 1 and Contrast Agent 2, there was no significant difference in the soil EC value compared with before application, indicating that the contrast agent has no salt-reducing effect. Only in the blank control group, the soil EC value increased after cotton harvest compared with before application. It shows that applying fertilizers 1# - 5# can significantly reduce the soil EC value, indicating that the fertilizers with the fertilizer composition containing Bacillus velezensis fermentation broth and calcium lignosulfonate have the effect of reducing soil salinity. Especially after applying fertilizer 3#, the soil EC value decreased from 2.80 to 1.75 before, and the effect on soil structure adjustment was significant.

[0080] Table 4 Test Results of the Effects of Different Treatments on Cotton Growth Promotion

[0081] Test grouping Number of cotton bolls per meter Single boll weight (g) Yield (kg) Yield increase rate (%) Fertilizer No. 1 132 6.38 376.54 31.89 Fertilizer No. 2 147 6.80 450.19 57.69 Fertilizer No. 3 161 7.34 526.08 84.27 Fertilizer No. 4 153 7.01 475.19 66.45 Fertilizer No. 5 149 6.75 441.53 54.66 Control agent No. 1 (CK1) 107 6.31 297.06 4.05 Control agent No. 2 (CK2) 104 6.25 287.52 0.71 Blank control (KC) 105 6.11 285.49 -

[0082] The test results of the fertilizers applying different fertilizer compositions containing the fermentation broth of Bacillus velezensis CGMCC No. 14384 and calcium lignosulfonate on the growth promotion of cotton are shown in Table 4. The cotton yield increase effects of applying Fertilizer 1# - 5# are obvious compared with Contrast Agent 1#, Contrast Agent 2# and the blank control. The yield increase ranges from 31.89% to 84.27% compared with the blank control. Especially, the yield increase amplitude of Fertilizer 3# is as high as 84.27%. There is no obvious difference between Contrast Agent 1 and Contrast Agent 2 compared with the blank control, and the growth promotion effect is not obvious, indicating that the fermentation broth of Bacillus velezensis CGMCC No. 14384 is mixed with calcium lignosulfonate compared with the fermentation broths of other types of Bacillus velezensis in the contrast agents. The mixed application of the fermentation broth of Bacillus velezensis CICC 10211 and the fermentation broth of Bacillus velezensis CGMCC No. 21866 with calcium lignosulfonate has limited growth promotion effect on cotton, and the growth promotion effect is far less than that of the mixed application of the fermentation broth of Bacillus velezensis CGMCC No. 14384 and calcium lignosulfonate. It shows that the fertilizer of the fertilizer composition containing the fermentation broth of Bacillus velezensis CGMCC No. 14384 and calcium lignosulfonate has a significant growth promotion effect on cotton. Among them, Fertilizer 3# of the fertilizer composition containing the fermentation broth of Bacillus velezensis CGMCC No. 14384 and fulvic acid is the best embodiment of the present invention.

[0083] The field test results of this embodiment show that the fertilizer of the fertilizer composition containing the fermentation broth of Bacillus velezensis CGMCC No. 14384 and calcium lignosulfonate not only has the effect of reducing soil salinity and alkalinity on saline-alkali soil, but also has an obvious effect on promoting the growth of saline-alkali soil plants. The fertilizer of the fertilizer composition containing the fermentation broth of Bacillus velezensis CGMCC No. 14384 and calcium lignosulfonate provided by the present invention can be applied to the growth promotion of saline-alkali soil plants and the improvement of saline-alkali soil.

[0084] As described above, the basic principles, main features and advantages of the present invention are preferably described. The above embodiments and the description are only for describing the preferred embodiments of the present invention. The present invention is not limited by the above embodiments. Without departing from the spirit and scope of the present invention, various changes and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the present invention.

Claims

1. A fertilizer composition containing Bacillus velezensis, characterized in that, The core components of the fertilizer composition are composed of the fermentation broth of Bacillus velezensis and calcium lignosulfonate; The preservation number of the Bacillus velezensis is CGMCC No. 14384.

2. The fertilizer composition containing Bacillus velezensis according to claim 1, characterized in that, The mass ratio of the Bacillus velezensis fermentation broth to calcium lignosulfonate is 0.5 - 20:1 - 15, and the concentration of Bacillus velezensis in the Bacillus velezensis fermentation broth is 10 8 cfu / mL.

3. The fertilizer composition containing Bacillus velezensis according to claim 2, wherein, The mass ratio of the fermentation broth of Bacillus velezensis to calcium lignosulfonate is 5:

5.

4. A fertilizer containing the fertilizer composition containing Bacillus velezensis according to any one of claims 1-3.

5. The fertilizer according to claim 4, characterized in that, The fertilizer contains fulvic acid.

6. Use of the fertilizer according to claim 4 in promoting plant growth, characterized in that, Applying the fertilizer during the plant growth cycle can promote plant growth.

7. The application according to claim 6, wherein The plant is a plant of the Solanaceae family or the Malvaceae family.

8. The application according to claim 7, wherein The Solanaceae plant is tomato, and the Malvaceae plant is cotton.

9. The application according to claim 8, characterized in that The application method of the fertilizer is drip irrigation, the application rate of the fertilizer is 2 L / mu / time, and the number of times of applying the fertilizer is 2 times during the plant growth period.

10. Use of the fertilizer according to claim 5 in improving the soil environment, characterized in that, The improvement of the soil environment is to reduce the soil salinity, and the reduction of the soil salinity is to reduce the soil EC value.

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