Nerve protection composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of nerve protection compositions, in particular to a nerve protection composition and a preparation method and application thereof. The preparation method comprises the following steps that S1, raw materials are prepared, specifically, the raw materials comprise theanine, chalcone, coumarin and flavonoid, and the mass ratio of theanine to chalcone to coumarin to flavonoid is 1: (0.4-0.6): (0.5-0.7): (0.4-0.6); s2, preparation of a solvent: the solvent is prepared by mixing acetonitrile and deionized water, and the mass ratio of the acetonitrile to the deionized water is 1: (0.7-0.8). According to the invention, theanine, chalcone, coumarin and flavonoids have anti-oxidation, anti-inflammatory and neuroprotective functions, theanine is used as a characteristic amino acid in tea leaves and has the functions of improving cognition, neuroprotection and the like, and theanine and chalcone, coumarin and flavonoids in angelica keiskei form a eutectic crystal, so that the curative effect of the tea is improved. The bioavailability and the treatment effect of the active ingredients are obviously improved.

Description

Technical Field

[0001] The present invention relates to the technical field of neuroprotective compositions, and in particular to a neuroprotective composition and a preparation method and application thereof. Background Art

[0002] Neuroprotective compositions are a type of substance combination that has the functions of protecting nerve cells and promoting the recovery of nerve function. They can be used to prevent and treat a variety of neurological diseases, such as Parkinson's disease and Alzheimer's disease.

[0003] In the prior art, neuroprotective compositions are typically prepared by extracting and preparing the ingredients based on their specific properties. However, when used alone, these ingredients suffer from low bioavailability and poor solubility, limiting their application in the treatment of neurodegenerative diseases. In light of this, the present invention provides a neuroprotective composition, its preparation method, and its application. Summary of the Invention

[0004] The purpose of the present invention is to provide a neuroprotective composition and its preparation method and application. The neuroprotective composition prepared by the present invention not only has good acetylcholinesterase inhibition performance, but also has excellent free radical scavenging performance, which effectively improves the performance of the neuroprotective composition.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a method for preparing a neuroprotective composition, comprising the following steps:

[0006] S1: preparing raw materials, including theanine, chalcone, coumarin and flavonoids, wherein the mass ratio of theanine, chalcone, coumarin and flavonoids is 1:(0.4-0.6):(0.5-0.7):(0.4-0.6);

[0007] S2: solvent preparation, the solvent is prepared by mixing acetonitrile and deionized water, and the mass ratio of acetonitrile to deionized water is 1: (0.7-0.8);

[0008] S3: Pretreatment of raw materials: theanine, chalcone, coumarin and flavonoids are pretreated separately, and the pretreated theanine, chalcone, coumarin and flavonoids are mixed to prepare a base material;

[0009] S4: preparing the composition, mixing the solvent and the base material to prepare the composition.

[0010] Furthermore, the pretreatment method of theanine is as follows: theanine is dried and ground, and after the grinding process, the mixture is added into a mixer, anhydrous ethanol is added into the mixer, and the mixer is set at 120 to 160 r / min and stirred for 20 to 30 minutes to complete the pretreatment of theanine.

[0011] Further, the temperature for the drying treatment of theanine is 40 - 50 °C, the treatment time is 20 - 30 min, the grinding particle size is 10 - 40 μm, and the mass of absolute ethanol is 4 - 6% of the mass of theanine.

[0012] Further, the pretreatment method of the chalcone is as follows: The chalcone and deionized water are mixed and added to a water bath. The water bath is set to heat up to 60 - 66 °C and keep warm for 6 - 10 min. During the heat preservation process, stirring is maintained. Then, the cooling rate is set to 1 - 3 °C / min, and the temperature is cooled to 16 - 20 °C and kept warm for 10 - 20 min. The obtained product is added to a centrifuge, and the centrifuge is set to centrifuge at 3000 - 5000 r / min for 4 - 6 min. Then, filtration treatment is carried out to collect the solid matter, and the pretreatment of the chalcone is completed. Among them, the mass ratio of the chalcone to the deionized water is 1:(1.2 - 1.4).

[0013] Further, the pretreatment method of the coumarin is as follows: The coumarin and magnesium stearate are mixed and then subjected to grinding treatment. Then, they are sent into an oven, and the oven is set to dry at 60 - 80 °C for 1 - 3 h to complete the pretreatment of the coumarin. Among them, the mass ratio of the coumarin to the magnesium stearate is 1:(0.2 - 0.4).

[0014] Further, the pretreatment method of the flavonoid is as follows: The flavonoid and palmitic acid ester are added to a water bath, and deionized water is added to the water bath. The water bath is set to heat up to 40 - 50 °C, and at the same time, a magnetic stirrer is connected, and the stirring speed is set to 60 - 80 r / min. The constant temperature stirring treatment is carried out for 8 - 12 min. Then, a sodium hydroxide solution is added dropwise to the water bath to adjust the pH value to 8.5. The obtained product is added to a centrifuge, and the centrifuge is set to centrifuge at 3000 - 5000 r / min for 6 - 10 min. The solid matter is taken from the centrifuged product, and the solid matter is added to an oven. The oven is set to 40 - 60 °C and dried for 3 - 5 h. The dried product is subjected to grinding treatment to complete the pretreatment of the flavonoid. Among them, the mass of the palmitic acid ester is 1 - 3% of the mass of the flavonoid, and the grinding particle size is 100 - 200 μm.

[0015] Further, the method for preparing the composition is as follows: The solvent and the base material are added to a water bath. The water bath is set to heat up to 45 - 55 °C, and at the same time, a magnetic stirrer is connected, and the stirring speed is set to 200 - 300 r / min. The constant temperature stirring treatment is carried out for 10 - 20 min. After the constant temperature stirring treatment is completed, after cooling to room temperature, the obtained product is sent into a reaction kettle. The reaction kettle is set to heat up at a rate of 2 - 4 °C / min and heat up to 60 °C, and keep warm for 12 - 14 min. Then, the cooling rate is set to 1 - 2 °C / min, and the temperature is cooled to 20 - 30 °C and kept warm for 6 - 10 min. Then, the cooling rate is set to 0.4 - 0.6 °C / min, and the temperature is cooled to 0 - 2 °C and kept warm for 12 - 14 h to obtain the crude material.

[0016] Furthermore, the coarse material is ground to obtain a neuroprotective composition with a grinding particle size of 60-100 μm.

[0017] Furthermore, a neuroprotective composition is prepared according to the preparation method of the neuroprotective composition.

[0018] Furthermore, an application of a neuroprotective composition, the application of the neuroprotective composition in the preparation of drugs for post-stroke nerve repair and chemotherapy-induced neuropathy.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] 1. In the present invention, after a neuroprotective composition is prepared with theanine, chalcone, coumarin and flavonoids as the base materials, theanine, chalcone, coumarin and flavonoids have antioxidant, anti-inflammatory and neuroprotective functions. As a characteristic amino acid in tea, theanine has functions such as improving cognition and neuroprotection. By forming a eutectic of theanine with chalcone, coumarin and flavonoids in Angelica keiskei, the bioavailability and therapeutic effect of the active ingredients are significantly improved.

[0021] 2. In the present invention, through the pretreatment steps of theanine, chalcone, coumarin and flavonoids in the preparation process, by pretreating theanine, chalcone, coumarin and flavonoids, the solubility and uniformity of each component in the mixed solvent of acetonitrile and water can be effectively improved, the crystallization process of the material can be optimized, and it can grow more orderly into high-purity, regular and complete crystals under a specific gradient cooling program, while reducing the influence of impurities on crystallization and product quality, so as to ensure the stable quality and consistent performance of the finally formed neuroprotective composition and achieve the best use effect. Detailed implementation manners

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0023] It should be noted that the raw materials used in the following embodiments are all commercially available raw materials.

[0024] Example 1: S1: Raw material preparation. The raw materials include theanine, chalcone, coumarin and flavonoids, and the mass ratio of theanine, chalcone, coumarin and flavonoids is 1:0.4:0.5:0.4;

[0025] S2: Solvent preparation. The solvent is prepared by mixing acetonitrile and deionized water, and the mass ratio of acetonitrile to deionized water is 1:0.7.

[0026] S3: Raw material pretreatment. Theanine, chalcone, coumarin and flavonoids are respectively pretreated, and after the pretreatment, theanine, chalcone, coumarin and flavonoids are mixed to obtain a base material.

[0027] S4: Composition preparation. The solvent and the base material are mixed to prepare a composition.

[0028] The pretreatment method of theanine is as follows: Theanine is dried and ground, and after the grinding treatment, it is added into a mixer. Absolute ethanol is added into the mixer, and the mixer is set to stir at 120 r / min for 20 min to complete the pretreatment of theanine.

[0029] The temperature of theanine drying treatment is 40 °C, the treatment time is 20 min, the grinding particle size is 10 μm, and the mass of absolute ethanol is 4% of the mass of theanine.

[0030] The pretreatment method of chalcone is as follows: Chalcone and deionized water are mixed and added into a water bath kettle. The water bath kettle is set to heat up to 60 °C and keep warm for 6 min. During the heat preservation process, stirring is maintained. Then, the temperature reduction rate is set to 1 °C / min, and the temperature is reduced to 16 °C and kept warm for 10 min. The obtained product is added into a centrifuge, and the centrifuge is set to centrifuge at 3000 r / min for 4 min. Then, filtration treatment is carried out to collect the solid matter to complete the pretreatment of chalcone. Among them, the mass ratio of chalcone to deionized water is 1:1.2.

[0031] The pretreatment method of coumarin is as follows: Coumarin and magnesium stearate are mixed and then ground, and then sent into an oven. The oven is set to dry at 60 °C for 1 h to complete the pretreatment of coumarin. Among them, the mass ratio of coumarin to magnesium stearate is 1:0.2.

[0032] The pretreatment method of flavonoids is as follows: Flavonoids and palmitic acid esters are added into a water bath kettle, deionized water is added into the water bath kettle, the water bath kettle is set to heat up to 40 °C, and at the same time, a magnetic stirrer is connected, and the stirring speed is set to 60 r / min for constant temperature stirring treatment for 8 min. Then, sodium hydroxide solution is added dropwise into the water bath kettle to adjust the pH value to 8.5. The obtained product is added into a centrifuge, and the centrifuge is set to centrifuge at 3000 r / min for 6 min. The solid matter is taken from the centrifuged product, the solid matter is added into an oven, the oven is set to 40 °C, and dried for 3 h. The dried product is ground to complete the pretreatment of flavonoids. Among them, the mass of palmitic acid esters is 1% of the mass of flavonoids, and the grinding particle size is 100 μm.

[0033] The method for preparing the composition is as follows: The solvent and the base material are added into a water bath kettle, and the water bath kettle is set to heat up to 50 °C. At the same time, a magnetic stirrer is connected, and the stirring speed is set to 200 r / min. After 10 min of constant-temperature stirring treatment, after the constant-temperature stirring treatment is completed, wait until it cools down to room temperature, and the obtained product is sent into a reaction kettle. The reaction kettle is set to have a heating rate of 2 °C / min and is heated up to 60 °C, and then kept warm for 12 min. After that, the cooling rate is set to 1 °C / min and cooled down to 20 °C, and then kept warm for 6 min. After that, the cooling rate is set to 0.4 °C / min and cooled down to 0 °C, and then kept warm for 12 h to obtain the crude material.

[0034] The crude material is ground to obtain the neuroprotective composition, and the grinding particle size is 60 μm.

[0035] Example 2: S1: Raw material preparation. The raw materials include theanine, chalcone, coumarin, and flavonoids. The mass ratio of theanine, chalcone, coumarin, and flavonoids is 1:0.5:0.6:0.5;

[0036] S2: Solvent preparation. The solvent is prepared by mixing acetonitrile and deionized water, and the mass ratio of acetonitrile and deionized water is 1:0.75;

[0037] S3: Raw material pretreatment. Theanine, chalcone, coumarin, and flavonoids are pretreated respectively. After the pretreatment, theanine, chalcone, coumarin, and flavonoids are mixed to obtain the base material;

[0038] S4: Composition preparation. The solvent and the base material are mixed to prepare the composition.

[0039] The pretreatment method of theanine is as follows: Theanine is dried and ground. After the grinding treatment, it is added into a mixer. Anhydrous ethanol is added into the mixer. The mixer is set to stir at 140 r / min for 25 min to complete the pretreatment of theanine.

[0040] The temperature of theanine drying treatment is 45 °C, the treatment time is 25 min, the grinding particle size is 20 μm, and the mass of anhydrous ethanol is 5% of the mass of theanine.

[0041] The pretreatment method of chalcone is as follows: Chalcone and deionized water are mixed and added into a water bath kettle. The water bath kettle is set to heat up to 63 °C and kept warm for 8 min. During the heat preservation process, the stirring state is maintained. After that, the cooling rate is set to 2 °C / min and cooled down to 18 °C, and then kept warm for 15 min. The obtained product is added into a centrifuge. The centrifuge is set to centrifuge at 3500 r / min for 5 min, and then filtered to collect the solid matter to complete the pretreatment of chalcone. Among them, the mass ratio of chalcone and deionized water is 1:1.3.

[0042] The pretreatment method of coumarin is as follows: Coumarin is mixed with magnesium stearate and then ground. After that, it is sent into an oven, and the oven is set to dry at 70°C for 2 hours to complete the pretreatment of coumarin. Among them, the mass ratio of coumarin to magnesium stearate is 1:0.3.

[0043] The pretreatment method of flavonoids is as follows: Flavonoids and palmitate are added into a water bath. Deionized water is added into the water bath. The water bath is set to heat up to 45°C, and at the same time, a magnetic stirrer is connected, and the stirring speed is set to 70 r / min. It is stirred at a constant temperature for 10 minutes. After that, sodium hydroxide solution is dropped into the water bath to adjust the pH value to 8.5. The obtained product is added into a centrifuge, and the centrifuge is set to centrifuge at 4000 r / min for 8 minutes. The solid matter is taken from the centrifuged product, and the solid matter is added into an oven. The oven is set to 50°C and dried for 4 hours. The dried product is ground to complete the pretreatment of flavonoids. Among them, the mass of palmitate is 2% of the mass of flavonoids, and the grinding particle size is 150 μm.

[0044] The method for preparing the composition is as follows: The solvent and the base material are added into a water bath. The water bath is set to heat up to 50°C, and at the same time, a magnetic stirrer is connected, and the stirring speed is set to 250 r / min. It is stirred at a constant temperature for 15 minutes. After completing the constant-temperature stirring treatment, after cooling to room temperature, the obtained product is sent into a reaction kettle. The reaction kettle is set to have a heating rate of 3°C / min and heated to 60°C, and kept warm for 13 minutes. After that, the cooling rate is set to 1.5°C / min and cooled to 25°C, and kept warm for 8 minutes. After that, the cooling rate is set to 0.5°C / min and cooled to 1°C, and kept warm for 13 hours to obtain the crude material.

[0045] The crude material is ground to obtain the neuroprotective composition, and the grinding particle size is 80 μm.

[0046] Example 3: S1: Raw material preparation. The raw materials include theanine, chalcone, coumarin and flavonoids. The mass ratio of theanine, chalcone, coumarin and flavonoids is 1:0.6:0.7:0.6;

[0047] S2: Solvent preparation. The solvent is prepared by mixing acetonitrile and deionized water. The mass ratio of acetonitrile to deionized water is 1:0.8;

[0048] S3: Raw material pretreatment. Theanine, chalcone, coumarin and flavonoids are pretreated respectively. After the pretreatment, theanine, chalcone, coumarin and flavonoids are mixed to obtain the base material;

[0049] S4: Composition preparation. The solvent and the base material are mixed to prepare the composition.

[0050] The pretreatment method of theanine is as follows: The theanine is dried and ground, and after the grinding process is completed, it is added into a mixer. Absolute ethanol is added into the mixer, and the mixer is set to stir at 160 r / min for 30 min to complete the pretreatment of theanine.

[0051] The temperature for the drying treatment of theanine is 50 °C, the treatment time is 30 min, the grinding particle size is 40 μm, and the mass of absolute ethanol is 6% of the mass of theanine.

[0052] The pretreatment method of chalcone is as follows: Chalcone and deionized water are mixed and added into a water bath kettle. The water bath kettle is set to heat up to 66 °C and keep warm for 10 min. During the heat preservation process, stirring is maintained. Then, the temperature reduction rate is set to 3 °C / min and cooled to 20 °C, and keep warm for 20 min. The obtained product is added into a centrifuge, and the centrifuge is set to centrifuge at 5000 r / min for 6 min. Then, filtration treatment is carried out to collect the solid matter to complete the pretreatment of chalcone. Among them, the mass ratio of chalcone to deionized water is 1:1.4.

[0053] The pretreatment method of coumarin is as follows: Coumarin and magnesium stearate are mixed and then ground. Then, it is sent into an oven, and the oven is set to dry at 80 °C for 3 h to complete the pretreatment of coumarin. Among them, the mass ratio of coumarin to magnesium stearate is 1:0.4.

[0054] The pretreatment method of flavonoid is as follows: Flavonoid and palmitic acid ester are added into a water bath kettle, and deionized water is added into the water bath kettle. The water bath kettle is set to heat up to 50 °C, and at the same time, a magnetic stirrer is connected, and the stirring speed is set to 80 r / min for constant temperature stirring treatment for 12 min. Then, sodium hydroxide solution is added dropwise into the water bath kettle to adjust the pH value to 8.5. The obtained product is added into a centrifuge, and the centrifuge is set to centrifuge at 5000 r / min for 10 min. The solid matter is taken from the centrifuged product, and the solid matter is added into an oven. The oven is set to 60 °C for drying treatment for 5 h. The dried product is ground to complete the pretreatment of flavonoid. Among them, the mass of palmitic acid ester is 3% of the mass of flavonoid, and the grinding particle size is 200 μm.

[0055] The method for preparing the composition is as follows: The solvent and the base material are added into a water bath kettle. The water bath kettle is set to heat up to 55 °C, and at the same time, a magnetic stirrer is connected, and the stirring speed is set to 300 r / min for constant temperature stirring treatment for 20 min. After the constant temperature stirring treatment is completed, wait until it cools down to room temperature. The obtained product is sent into a reaction kettle. The reaction kettle is set to heat up at a rate of 4 °C / min and heat up to 60 °C, and keep warm for 14 min. Then, the temperature reduction rate is set to 2 °C / min and cooled to 30 °C, and keep warm for 10 min. Then, the temperature reduction rate is set to 0.6 °C / min and cooled to 2 °C, and keep warm for 14 h to obtain the crude material.

[0056] The coarse materials are ground to obtain a neuroprotective composition with a grinding particle size of 100 μm.

[0057] Comparative Example 1: The difference between this comparative example and Example 1 is that in this comparative example, flavonoids were not pretreated.

[0058] Comparative Example 2: The difference between this comparative example and Example 1 is that in this comparative example, the raw materials were not pretreated.

[0059] Comparative Example 3: The difference between this comparative example and Example 1 is that this comparative example does not contain coumarin.

[0060] Comparative Example 4: The difference between this comparative example and Example 1 is that this comparative example does not contain theanine.

[0061] Performance test: Performance tests were conducted on the neuroprotective compositions prepared in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, and the test data obtained were recorded in the following table:

[0062]

[0063] It can be seen that the acetylcholinesterase inhibitory performance and free radical scavenging performance of the neuroprotective compositions prepared in Comparative Examples 1, 2, 3, and 4 are lower than those in Examples 1, 2, and 3; this shows that: after preparing the neuroprotective composition with theanine, chalcone, coumarin, and flavonoids as the base materials, theanine, chalcone, coumarin, and flavonoids have antioxidant, anti-inflammatory, and neuroprotective functions. Theanine, as a characteristic amino acid in tea, has functions such as improving cognition and neuroprotection. By forming a eutectic of theanine with the chalcone, coumarin, and flavonoids in Angelica keiskei, the bioavailability and therapeutic effect of the active ingredients are significantly improved;

[0064] Through the pretreatment steps of theanine, chalcone, coumarin, and flavonoids during the preparation process, by pretreating theanine, chalcone, coumarin, and flavonoids, the solubility and uniformity of each component in the mixed solvent of acetonitrile and water can be effectively improved, the crystallization process of the material can be optimized, so that it can grow more orderly into high-purity, regular and complete crystals under a specific gradient cooling program, and at the same time, the influence of impurities on crystallization and product quality can be reduced, thereby ensuring the stable quality and consistent performance of the finally formed neuroprotective composition and achieving the best use effect.

[0065] By comparing and analyzing the relevant data in the table, it can be seen that the neuroprotective composition prepared by the present invention not only has good acetylcholinesterase inhibitory performance, but also has excellent free radical scavenging performance. This shows that the neuroprotective composition provided by the present invention has a broader market prospect and is more suitable for promotion.

[0066] In the description of this specification, the descriptions referring to the terms "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0067] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and utilize the present invention well. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A method for preparing a neuroprotective composition, characterized in that: It includes the following steps: S1: Raw material preparation. The raw materials include L-theanine, chalcone, coumarin and flavonoids. The mass ratio of L-theanine, chalcone, coumarin and flavonoids is 1:(0.4 - 0.6):(0.5 - 0.7):(0.4 - 0.6); S2: Solvent preparation. The solvent is prepared by mixing acetonitrile and deionized water. The mass ratio of acetonitrile and deionized water is 1:(0.7 - 0.8); S3: Raw material pretreatment. L-theanine, chalcone, coumarin and flavonoids are pretreated respectively. After the pretreatment, L-theanine, chalcone, coumarin and flavonoids are mixed to obtain a base material; S4: Composition preparation. The solvent and the base material are mixed to prepare a composition.

2. The preparation method of a neuroprotective composition according to claim 1, wherein, The pretreatment method of the L-theanine is as follows: L-theanine is dried and ground. After the grinding treatment, it is added into a mixer. Anhydrous ethanol is added into the mixer. The mixer is set to stir at 120 - 160 r / min for 20 - 30 min to complete the pretreatment of L-theanine.

3. The preparation method of a neuroprotective composition according to claim 2, characterized in that, The temperature of the L-theanine drying treatment is 40 - 50 °C, the treatment time is 20 - 30 min, the grinding particle size is 10 - 40 μm, and the mass of anhydrous ethanol is 4 - 6% of the mass of L-theanine.

4. The preparation method of a neuroprotective composition according to claim 1, characterized in that, The pretreatment method of the chalcone is as follows: Chalcone and deionized water are mixed and added into a water bath. The water bath is set to heat up to 60 - 66 °C and keep warm for 6 - 10 min. Stirring is maintained during the warming process. Then the cooling rate is set to 1 - 3 °C / min and cooled to 16 - 20 °C, and keep warm for 10 - 20 min. The obtained product is added into a centrifuge. The centrifuge is set to centrifuge at 3000 - 5000 r / min for 4 - 6 min, and then filtered to collect the solid matter to complete the pretreatment of chalcone. Among them, the mass ratio of chalcone and deionized water is 1:(1.2 - 1.4).

5. The preparation method of a neuroprotective composition according to claim 1, characterized in that, The pretreatment method of the coumarin is as follows: Coumarin and magnesium stearate are mixed and then ground. Then it is sent into an oven. The oven is set to dry at 60 - 80 °C for 1 - 3 h to complete the pretreatment of coumarin. Among them, the mass ratio of coumarin and magnesium stearate is 1:(0.2 - 0.4).

6. The preparation method of a neuroprotective composition according to claim 1, wherein, The pretreatment method of the flavonoids is as follows: Flavonoids and palmitic acid ester are added into a water bath. Deionized water is added into the water bath. The water bath is set to heat up to 40 - 50 °C. At the same time, a magnetic stirrer is connected, and the stirring speed is set to 60 - 80 r / min, and keep stirring at a constant temperature for 8 - 12 min. Then sodium hydroxide solution is added dropwise into the water bath to adjust the pH value to 8.

5. The obtained product is added into a centrifuge. The centrifuge is set to centrifuge at 3000 - 5000 r / min for 6 - 10 min. The solid matter is taken from the centrifuged product. The solid matter is added into an oven. The oven is set to 40 - 60 °C and dried for 3 - 5 h. The dried product is ground to complete the pretreatment of flavonoids. Among them, the mass of palmitic acid ester is 1 - 3% of the mass of flavonoids, and the grinding particle size is 100 - 200 μm.

7. The preparation method of a neuroprotective composition according to claim 1, characterized in that, The method for preparing the composition is as follows: The solvent and the base material are added into a water bath kettle, and the water bath kettle is set to heat up to 45-55°C. Meanwhile, a magnetic stirrer is connected, and the stirring speed is set to 200-300 r / min. After constant-temperature stirring for 10-20 min, after the constant-temperature stirring treatment is completed, wait until it cools to room temperature, and the obtained product is sent into a reaction kettle. The reaction kettle is set to have a heating rate of 2-4°C / min and is heated to 60°C, and then kept warm for 12-14 min. After that, the cooling rate is set to 1-2°C / min, and it is cooled to 20-30°C and kept warm for 6-10 min. Then the cooling rate is set to 0.4-0.6°C / min, and it is cooled to 0-2°C and kept warm for 12-14 h to obtain the crude material.

8. The preparation method of a neuroprotective composition according to claim 7, characterized in that, The crude material is subjected to grinding treatment to obtain a neuroprotective composition, and the grinding particle size is 60-100 μm.

9. A neuroprotective composition, characterized in that, It is prepared according to the preparation method of the neuroprotective composition according to any one of claims 1-8.

10. Use of a neuroprotective composition according to claim 9, characterized in that, The application of the neuroprotective composition in the preparation of drugs for post-stroke nerve repair and chemotherapy-induced neuropathy.

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