Full-automatic multifunctional workstation

Abstract

The invention discloses a full-automatic multifunctional workstation, which relates to the field of biological experiment equipment, and comprises an automatic culture system and a safety cabinet which are arranged in a mutually isolated manner, and a transfer assembly is arranged between the automatic culture system and the safety cabinet to convey a target carrier; a plurality of mechanical arms are arranged at the top in the safety cabinet, a living cell detection assembly, a shaking table, a liquid adding assembly and other assemblies are arranged at the bottom of the safety cabinet, a pipetting system drives part of the assemblies to move, and the safety cabinet further comprises the liquid adding assembly with a heating piece, a switch cover assembly of a specific structure, a liquid suction assembly with a disposable suction head and the like. A waste treatment assembly and a disinfection assembly are arranged in the safety cabinet, and a consumable storage assembly and a cryopreservation assembly are arranged on one side of the safety cabinet, so that automatic operation of a biological experiment is achieved, multiple functions of culturing, observing, liquid adding, cover opening and closing, centrifuging and the like can be performed on a target carrier, and meanwhile, the functions of waste treatment, disinfection, consumable storage, sample cryopreservation and the like are achieved; and the experiment efficiency and the safety are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological experimental equipment, and more specifically, to a fully automatic multi-functional workstation. Background Art

[0002] In the current field of biological research, with the continuous growth of the number of domestic biological experiments, the demand for aspects such as cell culture, virus experiments, and bacteria experiments is increasing day by day. These experiments are of great significance in the medical field, such as assisting in disease diagnosis, optimizing treatment plans, promoting the development process of new drugs, and facilitating the development of the biotechnology industry. However, existing related products often have a single function and are difficult to meet the diversified market demands, making it difficult for researchers to carry out various biological experiments efficiently, and they are unable to complete a series of tasks from basic research to clinical application, seriously affecting the efficiency and progress of scientific research. This not only hinders the innovation and breakthrough of biomedicine but also is not conducive to enhancing China's competitiveness in the global biological research field. Therefore, the development of experimental equipment with multi-function and high efficiency has become an urgent need for the industry's development.

[0003] In the past, in cell culture, virus experiments, bacteria experiments, etc., manual culture methods were mostly used. Experimental personnel needed to manually complete cell passage operations, including a series of complex steps such as washing, digestion, centrifugation, dilution, etc., and transfer the cells to a new carrier; when observing the growth state of cells, live cell detection also needed to be carried out manually; operations such as opening and closing the lid of the carrier, adding culture medium, and aspirating waste liquid also relied on manual operation. In addition, manual culture also involved links such as sample storage and transportation, and each link required experimental personnel to do everything themselves. Although this traditional manual culture method can complete experimental tasks to a certain extent, it has become inadequate in the face of modern large-scale and high-precision biological experiment requirements.

[0004] The manual culture method has obvious defects. Since the experimental process requires a large number of repetitive operations, the work repetition rate is extremely high, which not only easily causes fatigue among experimental personnel but also increases the probability of errors. Moreover, the workload of manual culture is huge, consuming a large amount of time and energy of experimental personnel. In addition, the operating habits and standards of different experimental personnel may vary, and it is impossible to unify the culture specifications, thus affecting the accuracy and comparability of experimental results. At the same time, manual operation requires the use of a large number of consumables, resulting in a significant increase in the consumption of consumables and an increase in experimental costs. In addition, the speed of manual culture is relatively slow and difficult to meet the needs of large-scale experiments. And during the experiment, experimental personnel need to directly contact the target, which poses a certain danger and may pose a threat to their own health.

[0005] In summary, how to design a fully automatic multi-functional workstation that can replace manual labor, has the functions of automatic cell culture passage and experiments, and is compatible with multiple carriers is an urgent problem to be solved by those skilled in the art at present. Summary of the Invention

[0006] In view of this, the object of the present invention is to provide a fully automatic multi-functional workstation, which effectively solves the problems of high repeatability, low efficiency, and danger of manual operation in the field of biological experiments. Through the integration of an automated system and modular design, the full process automation of cell culture, virus experiments, and fungal experiments is realized.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A fully automatic multi-functional workstation includes an automatic culture system and a safety cabinet that are isolated from each other. A transfer component is provided between the automatic culture system and the safety cabinet to transfer the target carrier in the automatic culture system to the safety cabinet.

[0009] An installation space is provided in the safety cabinet. A plurality of robotic arms are provided at the top of the safety cabinet in the installation space. A live cell detection component for observing the state of the target carrier, a shaker for adjusting the angle of the target carrier, a liquid addition component for adding liquid to the target carrier, a lid opening and closing component for opening and closing the lid of the target carrier, a liquid suction component for sucking the waste liquid or separating the liquid of the target carrier, a centrifugation component for centrifugally extracting the target carrier, and an incubation component for heating and shaking the target carrier are provided at the bottom of the safety cabinet in the installation space.

[0010] A pipetting system is provided in the safety cabinet. The shaker, the liquid addition component, the liquid suction component, and the lid opening and closing component are integrated on the pipetting system. The pipetting system is used to drive the shaker, the liquid addition component, the liquid suction component, and the lid opening and closing component to move horizontally or vertically respectively.

[0011] Preferably, the liquid addition component includes a liquid addition pipeline and a heating element. The liquid addition pipeline is communicated with a container for storing the culture medium. The heating element is used to heat the culture medium in the liquid addition pipeline to a specified temperature.

[0012] Preferably, the pipetting system includes an electric slide rail and a plurality of lifting components provided on the electric slide rail. The plurality of lifting components are respectively connected to the shaker, the liquid addition component, the liquid suction component, and the lid opening and closing component.

[0013] Preferably, the lid opening and closing component includes a clamping member and a driving member. The clamping member is used to clamp the lid of the target carrier, and the driving member is used to drive the clamping member to perform the lid opening and closing operation.

[0014] Preferably, the liquid suction assembly includes a liquid suction gun disposed on one side of the pipetting system, and a detachable disposable pipette tip is configured on the liquid suction gun.

[0015] Preferably, the centrifugation assembly includes a centrifuge disposed in the safety cabinet.

[0016] Preferably, a waste treatment assembly is further disposed in the installation space where the safety cabinet is located. The waste treatment assembly includes an installation groove opened in the safety cabinet, a waste collection box disposed in the installation groove, and an automatic door disposed at the opening of the waste collection box.

[0017] Preferably, a disinfection assembly is disposed in the installation space where the safety cabinet is located. The disinfection assembly includes a plurality of disinfection spray heads disposed in the safety cabinet, and the plurality of disinfection spray heads are used for spraying disinfection inside the safety cabinet.

[0018] Preferably, a plurality of consumable preset racks are disposed in the safety cabinet, and consumables are placed in the plurality of consumable preset racks;

[0019] A consumable storage assembly is disposed on one side of the safety cabinet. The consumable storage assembly includes a rotary switching table and a plurality of consumable racks disposed along the circumference of the tabletop of the rotary switching table.

[0020] Preferably, a cryopreservation assembly is disposed on one side of the safety cabinet. The cryopreservation assembly includes a cryopreservation shell and cryopreservation tubes installed in the cryopreservation shell.

[0021] The full-automatic multi-functional workstation provided by the present invention, wherein the automatic culture system is isolated from the safety cabinet and the target carrier is transported through the transfer assembly, ensuring the safety of the operation environment; a plurality of robotic arms are arranged on the top of the safety cabinet, which can replace manual labor to complete tasks such as carrier transfer, improving work efficiency; the live cell detection assembly can observe the state of the target carrier, helping to master the experimental process; the shaker can adjust the angle of the target carrier, facilitating liquid suction and liquid addition; the liquid addition assembly can add liquid to the target carrier to meet the experimental requirements; the switch cover assembly can complete the switch cover operation of the target carrier; the liquid suction assembly can suck the waste liquid of the target carrier or perform liquid separation; the centrifugation assembly can perform centrifugal extraction on the target carrier; the incubation assembly can heat and shake the target carrier to accelerate processes such as digestion, mixing, and resuscitation; the pipetting system integrates the shaker, the liquid addition assembly, the liquid suction assembly, and the switch cover assembly, and can drive them to move horizontally or vertically, realizing the rapid installation and flexible operation of the multi-functional modules, improving the automation degree and compatibility of the workstation, and solving problems such as large workload and few functions existing in manual culture. Description of the Drawings

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on the provided drawings.

[0023] Figure 1 It is a schematic diagram of the overall structure of the fully automatic multifunctional workstation in this embodiment;

[0024] Figure 2 It is a top view of the fully automatic multifunctional workstation in this embodiment.

[0025] Figure 1 - Figure 2 Among them, the reference numerals include:

[0026] 1. Automatic culture system; 2. Safety cabinet; 3. Robot arm; 4. Live cell detection component; 5. Shaker; 6. Liquid addition component; 7. Liquid suction component; 8. Waste treatment component; 9. Switch cover component; 10. Centrifugation component; 11. Consumable pre-loading rack; 12. Pipetting system; 13. Disinfection component; 14. Consumable storage component; 15. Incubation component; 16. Cryopreservation component. Detailed implementation manners

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0028] Unless otherwise defined, the technical terms or scientific terms used in the disclosure of this application should have the ordinary meaning understood by those of ordinary skill in the art to which the present invention belongs. The "first", "second" and similar terms used in the present invention do not represent any order, quantity or importance. "Connection" or "connected" and similar terms are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Upper", "lower", "left", "right", etc. are only used to represent relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship can also change accordingly. This application embodiment discloses a fully automatic multifunctional workstation.

[0029] The core of the present invention is to provide a fully automatic multifunctional workstation.

[0030] Please refer to Figures 1 to 2 .

[0031] The fully automatic multi-functional workstation provided by the present invention includes an automatic culture system 1 and a safety cabinet 2 which are isolated from each other. A transfer component is provided between the automatic culture system 1 and the safety cabinet 2 to convey the target carrier in the automatic culture system 1 into the safety cabinet 2. An installation space is provided in the safety cabinet 2. A plurality of robotic arms 3 are provided at the top of the safety cabinet 2 in the installation space. A live cell detection component 4 for observing the state of the target carrier, a shaker 5 for adjusting the angle of the target carrier, a liquid adding component 6 for adding liquid to the target carrier, a lid opening and closing component 9 for opening and closing the lid of the target carrier, a liquid suction component 7 for sucking the waste liquid or separating the liquid of the target carrier, a centrifugation component 10 for centrifugally extracting the target carrier, and an incubation component 15 for heating and shaking the target carrier are provided at the bottom of the safety cabinet 2 in the installation space. A pipetting system 12 is provided in the safety cabinet 2. The shaker 5, the liquid adding component 6, the liquid suction component 7, and the lid opening and closing component 9 are integrated on the pipetting system 12. The pipetting system 12 is used to drive the shaker 5, the liquid adding component 6, the liquid suction component 7, and the lid opening and closing component 9 to move horizontally or vertically respectively.

[0032] Specifically, the automatic culture system 1 and the safety cabinet 2 are isolated from each other, and the target carrier in the automatic culture system 1 is conveyed into the safety cabinet 2 through the transfer component between them. A plurality of robotic arms 3 are provided at the top of the safety cabinet 2, and a live cell detection component 4, a shaker 5, a liquid adding component 6, a lid opening and closing component 9, a liquid suction component 7, a centrifugation component 10, and an incubation component 15 are provided at the bottom. The shaker 5, the liquid adding component 6, the liquid suction component 7, and the lid opening and closing component 9 are integrated on the pipetting system 12, and the pipetting system 12 can drive them to move horizontally or vertically respectively. This layout makes the components of the workstation have clear division of labor. The robotic arm 3 can simulate human hands to complete tasks such as carrier transfer. The live cell detection component 4 can observe the state of the target carrier in real time. The shaker 5 can adjust the angle of the carrier to facilitate liquid suction and liquid addition. The liquid adding component 6 can add liquid to the carrier. The lid opening and closing component 9 can complete the lid opening and closing operation of the carrier. The liquid suction component 7 can suck the waste liquid or separate the liquid. The centrifugation component 10 is used for centrifugally extracting the target substance. The incubation component 15 can heat and shake the target carrier. Moreover, the pipetting system 12 realizes the flexible adjustment of the positions of each component, so that the workstation can automatically complete multiple operations such as cell culture, virus experiments, and bacteria experiments, improving work efficiency, reducing manual labor intensity, and avoiding non-standard problems that may be caused by manual operations.

[0033] It should be noted that the automatic culture system 1 is a relatively independent area for the preliminary culture of the target carrier. It can adopt a suitable box structure, with a temperature and humidity control device, a lighting device, etc. installed inside to meet the culture conditions of different cells, viruses, microorganisms, etc. For example, the box can be made of high-strength plastic or metal and has good heat insulation performance. The temperature and humidity control device can include sensors and adjustment equipment, such as humidifiers, dehumidifiers, and thermostats, which can accurately control the temperature and humidity inside the box. The lighting device can provide appropriate lighting intensity and spectrum according to different culture requirements. The transfer component can be a conveyor belt or a manipulator, etc. If it is a conveyor belt, it can be driven by a motor and transport the target carrier from the automatic culture system to the safety cabinet through a belt. The surface of the conveyor belt can use anti-slip materials to ensure that the target carrier does not slip during transportation. If it is a manipulator, it can accurately grasp and place the target carrier through multi-joint movement and can adjust the grasping force according to needs.

[0034] It also should be noted that the safety cabinet 2 is the core working area of the entire workstation, which provides a safe environment for the internal components. The safety cabinet 2 adopts double front and rear electric lifting doors or a single-sided lifting door. The outer shell of the safety cabinet 2 can be made of stainless steel, with good corrosion resistance and sealing performance. The internal installation space is spacious, and various components can be reasonably arranged. Multiple robotic arms 3 are hoisted on the top of the safety cabinet. The robotic arm 3 can be a multi-joint industrial robotic arm, and different fixtures can be equipped at its end to adapt to different types of target carriers. For example, a jaw-type fixture can be used for culture bottles, and a suction cup-type fixture can be used for microplates. The robotic arm 3 is driven by a motor and a control system, and can accurately control its movement trajectory and force, so as to complete tasks such as carrier transfer, lid opening and closing, and consumable replacement.

[0035] In some other embodiments, the robotic arm 3 can also adopt a magnetically levitated driven robotic arm. The magnetically levitated driven robotic arm realizes movement through magnetic force, without traditional mechanical transmission components, reducing friction and wear, and improving the movement accuracy and reliability of the robotic arm 3. The joint part of the magnetically levitated robotic arm adopts a combination of electromagnetic coils and permanent magnets, and realizes the multi-degree-of-freedom movement of the robotic arm by controlling the magnitude and direction of the current in the electromagnetic coils.

[0036] Optionally, the transfer component adopts a pneumatic transmission device. The pneumatic transmission device includes pipelines, pneumatic valves, etc. The pipelines can be made of plastic or metal and have good airtightness. The pneumatic valves are controlled by air pressure and can quickly and accurately transmit the target carrier. When it is necessary to transfer the target carrier from the automatic culture system to the safety cabinet, by adjusting the air pressure and opening the corresponding pneumatic valve, the target carrier moves quickly along the pipeline into the safety cabinet under the action of the air flow. This method has the advantages of fast transmission speed and high accuracy, and can further improve the working efficiency of the workstation.

[0037] In the above-mentioned fully automatic multi-functional workstation, the automatic culture system 1 and the safety cabinet 2 are isolated from each other and the target carrier is transported through the transfer component, ensuring the safety of the operating environment; multiple robotic arms 3 are arranged on the top of the safety cabinet 2, which can replace manual labor to complete tasks such as carrier transfer, improving work efficiency; the live cell detection component 4 can observe the state of the target carrier, helping to master the experimental process; the shaker 5 can adjust the angle of the target carrier, facilitating liquid suction and liquid addition; the liquid addition component 6 can add liquid to the target carrier to meet the experimental requirements; the switch cover component 9 can complete the operation of opening and closing the cover of the target carrier; the liquid suction component 7 can suck the waste liquid of the target carrier or perform liquid separation; the centrifugation component 10 can perform centrifugal extraction on the target carrier; the incubation component 15 can heat and shake the target carrier to accelerate processes such as digestion, mixing, and resuscitation; the pipetting system 12 integrates the shaker 5, the liquid addition component 6, the liquid suction component 7, and the switch cover component 9, and can drive them to move horizontally or vertically, realizing the rapid installation and flexible operation of the multi-functional modules, improving the automation degree and compatibility of the workstation, and solving problems such as large workload and few functions in manual culture.

[0038] The following will introduce the fully automatic multi-functional workstation provided by the present invention in more detail with reference to the accompanying drawings and specific embodiments.

[0039] In a specific embodiment, the live cell detection component 4 is used to observe the growth state of cells in the target carrier, and it can be equipment such as a microscope. The microscope can be an electron microscope or an optical microscope, with high resolution and magnification, and can clearly observe the morphology and structure of cells. The microscope can be installed on an adjustable bracket to adjust the observation angle and position.

[0040] In a specific embodiment, the shaker 5 is used to adjust the angle of the target carrier, facilitating liquid suction and liquid addition operations. The shaker 5 can be an electric shaker, driven by a motor, and can achieve different shaking modes and speeds. For example, it can perform circular shaking, reciprocating shaking, etc. The platform of the shaker can be made of rubber or silica gel to increase friction and prevent the target carrier from sliding.

[0041] In a specific embodiment, refer to Figure 2, the liquid adding assembly 6 includes a liquid adding pipeline and a heating element. The liquid adding pipeline is communicated with a container for storing the culture medium, and the heating element is used to heat the culture medium in the liquid adding pipeline to a specified temperature.

[0042] Specifically, the liquid adding pipeline of the liquid adding assembly 6 is communicated with the container for storing the culture medium, and the heating element is matched with the liquid adding pipeline. After the culture medium enters the liquid adding pipeline from the container, the heating element can heat the culture medium in the liquid adding pipeline to a specified temperature. This structure enables the liquid adding assembly to provide the culture medium at an appropriate temperature to be added to the target carrier, avoiding affecting the growth environment of cells, viruses or bacteria in the target carrier due to inappropriate temperature of the culture medium, and thus improving the success rate of culture and experiment.

[0043] The liquid adding assembly 6 instantaneously heats the culture medium stored at a low temperature in the container for storing the culture medium to a suitable temperature and adds it to the carrier through the output port. The liquid adding pipeline can be made of stainless steel pipeline, which has good corrosion resistance and hygiene, can be reused, and is consumable-free. The heating element can be an electric heating wire or a heating sheet, which is wrapped outside the liquid adding pipeline, and the heating temperature of the culture medium is precisely controlled through a temperature sensor and a control system.

[0044] Optionally, the heating element adopts a microwave heating method. The microwave heating method can heat the culture medium quickly and evenly, improving the heating efficiency. The microwave generated by the microwave generator directly acts on the culture medium in the liquid adding pipeline, causing the culture medium molecules to vibrate rapidly to generate heat. At the same time, the heating time and power are precisely controlled through a temperature sensor and a control system to avoid overheating of the culture medium.

[0045] Based on any one of the above embodiments, refer to Figure 2 , the liquid transfer system 12 includes an electric slide rail and a plurality of lifting assemblies arranged on the electric slide rail. The plurality of lifting assemblies are respectively connected to the shaker 5, the liquid adding assembly 6, the liquid suction assembly 7 and the switch cover assembly 9.

[0046] Specifically, the electric slide rail of the liquid transfer system 12 is arranged in the safety cabinet 2, and the plurality of lifting assemblies are arranged on the electric slide rail and are respectively connected to the shaker 5, the liquid adding assembly 6, the liquid suction assembly 7 and the switch cover assembly 9. The electric slide rail can drive the lifting assemblies to move horizontally, and the lifting assemblies themselves can drive the connected shaker 5, liquid adding assembly 6, liquid suction assembly 7 and switch cover assembly 9 to move vertically. This enables the shaker 5, liquid adding assembly 6, liquid suction assembly 7 and switch cover assembly 9 to move flexibly in the horizontal and vertical directions, and thus accurately and efficiently complete operations such as liquid adding, liquid suction and switch cover of the target carrier, effectively improving the working efficiency and operation accuracy of the workstation. It enables each component to flexibly reach the operation position required by the target carrier, enhancing the operation flexibility and accuracy, and further improving the automation degree and working efficiency of the workstation.

[0047] It should be noted that the electric slide rail can be a linear guide rail, and the slider is driven by a motor to slide on the guide rail. The lifting assembly can be a screw lift or a cylinder, and the height of each component is adjusted through lifting motion. The pipetting system 12 enables each component to move flexibly in the horizontal and vertical directions, improving the working efficiency and flexibility of the workstation.

[0048] Based on any one of the above embodiments, referring to Figure 2 , the switch cover assembly 9 includes a clamping member and a driving member. The clamping member is used to clamp the cover of the target carrier, and the driving member is used to drive the clamping member to perform the cover opening and closing operation.

[0049] Specifically, the switch cover assembly 9 is arranged in the installation space of the safety cabinet and integrated on the pipetting system 12. The pipetting system can drive it to move horizontally or vertically. The switch cover assembly 9 includes a clamping member for clamping the cover of the target carrier and a driving member for driving the clamping member to perform the cover opening and closing operation. The driving member drives the clamping member to act, which can realize the automatic opening and closing of the cover of the target carrier, avoid manual operation, reduce the workload, improve the working efficiency, and ensure the unity of operation specifications.

[0050] It should be noted that the clamping member can be a jaw, which is made of an elastic material to avoid damaging the cover. The driving member can be a motor or a cylinder, and the clamping member is driven to move through a transmission mechanism.

[0051] Based on any one of the above embodiments, referring to Figure 2 , the liquid suction assembly 7 includes a liquid suction gun arranged on one side of the pipetting system 12, and a detachable disposable pipette tip is configured on the liquid suction gun.

[0052] Specifically, the liquid suction gun is arranged on one side of the pipetting system 12, and a detachable disposable pipette tip is configured on it. When it is necessary to suck the waste liquid of the target carrier or perform liquid separation, the operation can be completed by the liquid suction gun in cooperation with the disposable pipette tip. After use, the disposable pipette tip can be disassembled and replaced to avoid cross-contamination and ensure the safety and accuracy of the liquid suction operation. The liquid suction gun can be an electric liquid suction gun, and the waste liquid in the target carrier is sucked or the liquid separation operation is performed through the negative pressure principle. The disposable pipette tip can be replaced according to needs to ensure hygiene and the accuracy of the experiment.

[0053] Based on any one of the above embodiments, referring to Figure 2 , the centrifugation assembly 10 includes a centrifuge arranged in the safety cabinet 2.

[0054] Specifically, the centrifuge is arranged inside the safety cabinet and can be used to perform centrifugal extraction on the target carrier. After the target carrier is sent to the centrifuge, the centrifuge runs at high speed, which can effectively separate the target from the carrier, facilitating subsequent research or experimental operations on the target, avoiding the cumbersome and uncertain manual operations, and improving work efficiency and accuracy. The centrifuge can be a high-speed centrifuge, which can provide sufficient centrifugal force to separate the substances in the target carrier. The rotor of the centrifuge can be selected in different specifications according to needs to adapt to different types of target carriers.

[0055] Based on any of the above embodiments, with reference to Figure 2 , a waste treatment component 8 is further arranged inside the installation space where the safety cabinet 2 is located. The waste treatment component 8 includes an installation groove opened inside the safety cabinet 2, a waste collection box arranged inside the installation groove, and an automatic door arranged at the opening of the waste collection box.

[0056] Specifically, the waste treatment component 8 includes an installation groove opened inside the safety cabinet. A waste collection box is arranged inside the installation groove, and an automatic door is arranged at the opening of the waste collection box. When waste needs to be treated, the automatic door automatically opens, and the waste can be thrown into the waste collection box, which can effectively collect and treat the waste centrally and avoid the pollution to the working environment caused by the random placement of waste.

[0057] It should be noted that the automatic door can be electrically controlled. When the robotic arm grabs the waste and approaches, it can automatically sense and open, so that the waste can be smoothly put into the waste collection box; it can also be controlled by a sensor, such as an infrared sensor, etc. When it senses the approach of the waste, it automatically opens the box opening and automatically closes after the waste is put in, thus effectively avoiding the pollution caused by the exposure of the waste inside the safety cabinet, keeping the environment inside the safety cabinet clean, and ensuring the safety and accuracy of the experiment.

[0058] Based on any of the above embodiments, with reference to Figure 1 , a disinfection component 13 is arranged inside the installation space where the safety cabinet 2 is located. The disinfection component 13 includes a plurality of disinfection spray heads arranged inside the safety cabinet 2, and the plurality of disinfection spray heads are used for spraying disinfection inside the safety cabinet 2.

[0059] Specifically, a disinfection component 13 is arranged inside the installation space of the safety cabinet. The disinfection component 13 is composed of a plurality of disinfection spray heads arranged inside the safety cabinet. These disinfection spray heads can directly spray and disinfect the inside of the safety cabinet, mainly aiming at the waste collection box, the pipetting system 12, the spraying area inside the safety cabinet 2, etc. Through such a position setting and working mode, it can effectively disinfect the waste, waste liquid, consumables, etc. inside the safety cabinet, prevent pollution, and ensure the safety and cleanliness of the experimental environment. The disinfection spray heads can adopt atomizing spray heads to evenly spray the disinfectant inside the safety cabinet 2.

[0060] Based on any of the above embodiments, with reference to Figure 1 , a plurality of consumable preset racks 11 are provided in the safety cabinet 2, and consumables are placed in the plurality of consumable preset racks 11; a consumable storage component 14 is provided on one side of the safety cabinet 2, and the consumable storage component 14 includes a rotary switching table and a plurality of consumable racks arranged along the circumference of the tabletop of the rotary switching table.

[0061] Specifically, the consumable preset rack 11 is used to place consumables (such as disposable tips of the above pipettes) and target objects, etc. The consumable storage component 14 is used to store various consumables required for experiments and provide consumables for the entire workstation. A plurality of consumable preset racks 11 for placing consumables are provided in the safety cabinet. The consumable preset racks 11 can be provided at the bottom of the safety cabinet 2 or on the side wall of the safety cabinet 2. A consumable storage component 14 is provided on one side of the safety cabinet, and this component includes a rotary switching table and a plurality of consumable racks arranged along the circumference of its tabletop. Through this layout, the consumable storage component 14 can use the rotary switching table to switch different consumable racks to replenish the required consumables for the workstation, and the consumable preset rack 11 can provide ready-made consumables for in-station operations at any time, enabling the workstation to obtain various consumables in a timely manner during operation and ensuring the smooth and efficient progress of experimental work.

[0062] Based on any of the above embodiments, with reference to Figure 2 , a cryopreservation component 16 is provided on one side of the safety cabinet 2, and the cryopreservation component 16 includes a cryopreservation shell and cryopreservation tubes installed in the cryopreservation shell.

[0063] Specifically, the cryopreservation component is provided on one side of the safety cabinet. The cryopreservation component includes a cryopreservation shell and cryopreservation tubes installed in the cryopreservation shell, and can be used for cryopreservation of target objects, enabling the workstation to meet the need for ultra-low temperature cryopreservation of target objects and enhancing the diversity and comprehensiveness of the workstation functions.

[0064] Based on any of the above embodiments, the incubation component 15 is used to heat and shake the target carrier to accelerate the digestion, mixing, resuscitation, etc. of the target carrier. The incubation component 15 can be a box with heating and shaking functions, and is internally provided with a temperature sensor and a shaking device, capable of precisely controlling the temperature and shaking frequency.

[0065] The implementation principle of an all - automatic multi - functional workstation in an embodiment of this application is as follows: By isolating the automatic culture system 1 and the safety cabinet 2 from each other and using a transfer component to achieve the transportation of the target carrier, the independence and safety of the culture environment are ensured. Multiple components in the safety cabinet 2 work together. The robotic arm 3 is responsible for the handling and operation of the carrier, the live cell detection component 4 provides real - time feedback on the cell state, the shaker 5, the liquid addition component 6, the liquid suction component 7, the lid opening / closing component 9, the centrifugation component 10, and the incubation component 15 respectively complete their respective functions, the waste treatment component 8 processes waste, the disinfection component 13 performs disinfection, the consumable pre - setting rack 11 and the consumable storage component 14 provide the required consumables, the cryopreservation component 16 performs cryopreservation of the target, and the pipetting system 12 provides support for the movement of each component. This way of clear division of labor and collaborative cooperation effectively solves the problems in the manual culture method, such as high work repetition, large workload, inability to unify culture specifications, large consumption of consumables, slow speed, high risk, etc., greatly improving work efficiency and experimental quality, and bringing convenience and innovation to the field of biological experiments.

[0066] Another core of the present invention is to provide a usage method of an all - automatic multi - functional workstation, which is applied to the above - mentioned all - automatic multi - functional workstation.

[0067] The usage method of the all - automatic multi - functional workstation provided by the present invention includes the following steps;

[0068] S1. Place the target carrier in the automatic culture system 1. Through program setting, use a transfer component (such as a conveyor belt, a robotic arm, or a pneumatic transmission device, etc.) to transport the target carrier from the automatic culture system 1 to the safety cabinet 2. The robotic arm 3 inside the safety cabinet 2 grabs the target carrier and sends it to the live cell detection component 4 for observation to understand the growth state of the cells in the target carrier.

[0069] S2. Taking cell passage as an example, the robotic arm 3 moves the target carrier to the lid opening / closing component 9 for the lid - opening operation. Then, move the carrier after lid - opening to the liquid suction area. At this time, the pipetting system 12 drives the liquid suction component 7 to move to a suitable position. The pipette on the liquid suction component 7 uses the negative pressure principle to suck the old culture medium or other waste liquid in the target carrier through a detachable disposable pipette tip.

[0070] S3. After liquid suction is completed, the robotic arm 3 moves the carrier to the liquid addition position, and the pipetting system 12 drives the liquid addition component 6 to move into place. The liquid addition pipeline of the liquid addition component 6 is connected to the container storing the culture medium. The heating element (such as an electric heating wire, a heating sheet, or using microwave heating) heats the culture medium in the liquid addition pipeline to the specified temperature and then adds the culture medium into the target carrier through the liquid addition pipeline. After liquid addition is completed, the robotic arm 3 moves the carrier to the lid opening / closing component 9 for the lid - closing operation.

[0071] In S4, the robotic arm 3 moves the carrier with the lid on to the shaker 5 or the incubation assembly 15. The shaker 5 can be driven by a motor to adjust the angle of the target carrier, facilitating subsequent liquid aspiration and liquid addition operations. It can also perform circular shaking, reciprocating shaking, etc., to fully mix the culture medium and the target substance. The incubation assembly 15 can heat and agitate the target carrier to accelerate the digestion, mixing, resuscitation, etc. of the target carrier.

[0072] In S5, the operation of aspirating and discarding the waste liquid is performed again. The robotic arm 3 moves the carrier to the liquid addition position to add liquid again, and then moves to the lid opening and closing assembly 9 to open and close the lid, so that the culture medium is quickly spread evenly to ensure full contact with the surface of the target substance.

[0073] In S6, for the centrifugation operation involved in the cell passage process, the robotic arm 3 sends the centrifuge tube to the lid opening and closing assembly 9 to open the lid. After the shaker 5 adjusts the angle, the robotic arm 3 places the centrifuge tube on the liquid transfer system 12. The liquid aspiration assembly 7 transfers the cell suspension in the carrier into the centrifuge tube. Subsequently, the robotic arm 3 sends it to the lid opening and closing assembly 9 to screw on the lid. After centrifugation, the robotic arm 3 sends the centrifuge tube to the lid opening and closing assembly 9 to open the lid and places it on the liquid transfer system 12. The liquid transfer system 12 drives the liquid aspiration assembly 7 to complete the operation of aspirating the supernatant and leaving the cell pellet. Then, the robotic arm 3 moves the centrifuge tube to the liquid addition position to add the culture medium and shake it to mix evenly.

[0074] In S7, the robotic arm 3 takes a new carrier to the liquid addition area to add liquid, adds an appropriate amount of complete culture medium, and places it on the shaker 5. The liquid transfer system 12 drives the liquid aspiration assembly 7 to complete the addition of the cell suspension, and the shaker 5 shakes the carrier to mix the cells. After that, the robotic arm 3 moves the waste centrifuge tube to the lid opening and closing assembly 9 to screw on the lid and throws it into the waste collection box of the waste treatment assembly 8.

[0075] In S8, the robotic arm 3 sends the new carrier to the microscope at the live cell detection assembly 4 for observation. If the observation result is qualified, the robotic arm 3 places the new carrier on the external bracket of the incubator, and the internal robotic arm 3 of the incubator grabs and places it on the bracket for cultivation. If it is unqualified, the carrier is discarded, and it can be passaged again or other experimental operations can be performed. During the whole process, multiple disinfection nozzles of the disinfection assembly 13 will spray and disinfect the safety cabinet 2 in a timely manner to disinfect the waste, waste liquid, consumables, etc. The consumable pre-setting rack 11 and the consumable storage assembly 14 provide various consumables required for the experiment.

[0076] The usage method of the above-mentioned fully automatic multi-functional workstation is based on the collaborative work of each component of the fully automatic multi-functional workstation, and operations such as cell culture, subculture, and experiments are sequentially completed according to preset steps. The manipulator 3 is used to carry and operate the carrier, and each component flexibly moves under the drive of the liquid transfer system 12 to achieve functions such as accurate liquid addition, liquid suction, lid opening and closing, centrifugation, incubation, etc. At the same time, the live cell detection component 4 monitors the cell state in real time, the waste treatment component 8 processes waste, the disinfection component 13 ensures the hygiene and safety of the experimental environment, and the consumable pre-setting rack 11 and the consumable storage component 14 provide sufficient consumable support. This automated operation process effectively avoids the drawbacks of the manual culture method, improves work efficiency, the accuracy and standardization of experiments, reduces experimental risks, and provides a more efficient and reliable solution for biological experiments.

[0077] In this specification, each embodiment is described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. For the same or similar parts among the embodiments, reference can be made to each other.

[0078] The above has introduced in detail a fully automatic multi-functional workstation provided by the present invention. Specific examples are used in this article to elaborate on the principle and implementation manner of the present invention. The description of the above embodiments is only used to help understand the method and its core idea of the present invention. It should be noted that for those of ordinary skill in the art of this technology, without departing from the principle of the present invention, several improvements and modifications can still be made to the present invention, and these improvements and modifications also fall within the protection scope of the present invention.

Claims

1. A fully automatic multi-functional workstation, characterized in that, It includes an automatic culture system (1) and a safety cabinet (2) which are isolated from each other. A transfer component is arranged between the automatic culture system (1) and the safety cabinet (2) to convey the target carrier in the automatic culture system (1) into the safety cabinet (2). An installation space is provided in the safety cabinet (2). A plurality of robotic arms (3) are arranged at the top of the safety cabinet (2) in the installation space. At the bottom of the safety cabinet (2) in the installation space, there are a live cell detection component (4) for observing the state of the target carrier, a shaker (5) for adjusting the angle of the target carrier, a liquid adding component (6) for adding liquid to the target carrier, a lid opening and closing component (9) for opening and closing the lid of the target carrier, a liquid suction component (7) for sucking the waste liquid or separating the liquid of the target carrier, a centrifugation component (10) for centrifugally extracting the target carrier, and an incubation component (15) for heating and shaking the target carrier. A pipetting system (12) is arranged in the safety cabinet (2). The shaker (5), the liquid adding component (6), the liquid suction component (7), and the lid opening and closing component (9) are integrated on the pipetting system (12). The pipetting system (12) is used to drive the shaker (5), the liquid adding component (6), the liquid suction component (7), and the lid opening and closing component (9) to move horizontally or vertically respectively.

2. The fully automatic multi-functional workstation according to claim 1, wherein The liquid adding component (6) includes a liquid adding pipe and a heating element. The liquid adding pipe is communicated with a container for storing the culture medium. The heating element is used to heat the culture medium in the liquid adding pipe to a specified temperature.

3. A fully automatic multi-functional workstation according to claim 1, characterized in that, 4. The full-automatic multi-functional workstation according to claim 1, characterized in that, The pipetting system (12) includes an electric slide rail and a plurality of lifting components arranged on the electric slide rail. The plurality of lifting components are respectively connected to the shaker (5), the liquid adding component (6), the liquid suction component (7), and the lid opening and closing component (9).

5. A fully automatic multi-functional workstation according to claim 1, characterized in that, The lid opening and closing component (9) includes a clamping member and a driving member. The clamping member is used to clamp the lid of the target carrier. The driving member is used to drive the clamping member to perform the lid opening and closing operation.

6. A fully automatic multi-functional workstation according to claim 1, characterized in that, The liquid suction component (7) includes a pipette gun arranged on one side of the pipetting system (12). A detachable disposable pipette tip is configured on the pipette gun.

7. A fully automatic multi-functional workstation according to any one of claims 1-6, characterized in that, The centrifugation component (10) includes a centrifuge arranged in the safety cabinet (2).

8. A fully automatic multi-functional workstation according to any one of claims 1-6, characterized in that, A waste treatment component (8) is further arranged in the installation space of the safety cabinet (2). The waste treatment component (8) includes an installation groove opened in the safety cabinet (2), a waste collection box arranged in the installation groove, and an automatic door arranged at the opening of the waste collection box.

9. A fully automatic multi-functional workstation according to any one of claims 1-7, characterized in that, A disinfection component (13) is arranged in the installation space of the safety cabinet (2). The disinfection component (13) includes a plurality of disinfection spray heads arranged in the safety cabinet (2). The plurality of disinfection spray heads are used to spray disinfectant in the safety cabinet (2). A plurality of consumable preset racks (11) are arranged in the safety cabinet (2). Consumables are placed in the plurality of consumable preset racks (11). On one side of the safety cabinet (2), a consumable storage component (14) is provided. The consumable storage component (14) includes a rotary switching table and a plurality of consumable racks arranged circumferentially along the tabletop of the rotary switching table.

10. A fully automatic multi-functional workstation according to any one of claims 1-7, characterized in that, On one side of the safety cabinet (2), a cryopreservation component (16) is provided. The cryopreservation component (16) includes a cryopreservation shell and cryopreservation tubes installed in the cryopreservation shell.

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