Biological agent containing lactobacillus plantarum HP-ZW2 and preparation method thereof

Abstract

The invention relates to the technical field of biology, and particularly discloses a biological agent containing lactobacillus plantarum HP-ZW2 and a preparation method of the biological agent. The invention discloses a biological preparation containing lactobacillus plantarum HP-ZW2. The biological preparation is prepared from the following raw materials in parts by weight: 16 to 20 parts of lactobacillus plantarum HP-ZW2 bacterial sludge, 50 to 60 parts of starch, 20 to 25 parts of maltodextrin, 27 to 30 parts of modified activated carbon, 10 to 15 parts of skim milk powder, 23 to 26 parts of modified ceramic particles, 6 to 10 parts of trehalose, 3 to 6 parts of sucrose, 7 to 10 parts of trace element salt and 190 to 200 parts of purified water. The prepared biological agent containing lactobacillus plantarum HP-ZW2 can effectively adsorb organic matters, peculiar smell, pigments and heavy metal ions in water, improve the water quality and improve the sewage treatment efficiency.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and more specifically, it relates to a biological preparation containing Lactobacillus plantarum HP-ZW2 and a preparation method thereof. Background Art

[0002] Lactobacillus plantarum belongs to the genus Lactobacillus in the family Lactobacillaceae, is a Gram-positive bacterium, facultatively anaerobic, and is a probiotic group in the human gastrointestinal tract. It can maintain the stability of the intestinal environment pH, which is beneficial to preventing the invasion and growth of pathogenic bacteria. In aquaculture, it can purify water quality, decompose organic matter at the bottom of the pond, remove odors, eliminate algal toxins, and create a good habitat environment for aquatic organisms; at the same time, it can also degrade harmful substances such as ammonia nitrogen and nitrite in the water body, reduce the organic oxygen consumption, indirectly increase oxygen, and improve water quality.

[0003] In aquaculture, Lactobacillus plantarum is usually mixed with other beneficial bacteria (such as Bacillus, photosynthetic bacteria, denitrifying bacteria, etc.) to promote the large-scale reproduction and growth of bait organisms in the aquaculture water body, and at the same time decompose residual baits, feces and organic matter in the water, improve the water environment, and inhibit the reproduction and growth of harmful bacteria in the water body.

[0004] Although Lactobacillus plantarum has a good degradation effect on harmful substances such as ammonia nitrogen and nitrite, its removal effect on polycyclic aromatic hydrocarbons, organochlorine pesticides, and heavy metal ions may be limited. Summary of the Invention

[0005] In order to improve the problem of poor removal effect of polycyclic aromatic hydrocarbons, organochlorine pesticides, and heavy metal ions, the present application provides a biological preparation containing Lactobacillus plantarum HP-ZW2 and a preparation method thereof.

[0006] In a first aspect, the present application provides a biological preparation containing Lactobacillus plantarum HP-ZW2, which comprises the following raw materials in parts by weight: 16-20 parts of Lactobacillus plantarum HP-ZW2 bacterial sludge, 50-60 parts of starch, 20-25 parts of maltodextrin, 27-30 parts of modified activated carbon, 10-15 parts of skim milk powder, 23-26 parts of modified ceramic particles, 6-10 parts of trehalose, 3-6 parts of sucrose, 7-10 parts of trace element salts, and 190-200 parts of purified water.

[0007] By adopting the above technical solutions, the Lactobacillus plantarum HP-ZW2 bacterial sludge, as a beneficial microorganism, can decompose the organic matter in water, jointly maintain the ecological balance of the water body with other microorganisms in the water, and improve water quality. Starch provides rich carbon sources and energy, stimulates the growth and reproduction of microorganisms such as bacteria and algae, promotes the growth and metabolism of microorganisms in sewage treatment, and improves sewage treatment efficiency. Maltodextrin has excellent adsorption properties and can complex with pollutants such as organic matter and heavy metal ions in sewage, thereby effectively removing these harmful substances. Modified activated carbon adsorbs organic matter, odors, pigments and heavy metal ions in water, improves water quality, and skimmed milk powder provides rich nutrients such as vitamins, proteins and fats, improving the water environment.

[0008] The modified ceramic particles serve as a biological carrier, providing an environment for the attachment and growth of microorganisms, promoting the formation of biofilms, and enhancing biodegradation ability. Trehalose has certain biocompatibility and stability in sewage treatment, helping to maintain the activity of microorganisms. Sucrose increases the number of probiotics in the intestines of fish, inhibits the growth of pathogenic bacteria, enhances the function of the immune system, and provides the energy and carbon sources required for growth. Trace element salts provide the trace elements required for the enzyme activity of microorganisms, promoting the growth and metabolism of microorganisms. Mixing these components and using them for the sewage treatment after aquaculture can play a synergistic role, improving sewage treatment efficiency and water quality.

[0009] Optionally, the preparation method of the Lactobacillus plantarum HP-ZW2 bacterial sludge includes the following steps: inoculating the Lactobacillus plantarum seed liquid into a fermentation medium for fermentation to obtain a Lactobacillus plantarum fermentation liquid, centrifuging the prepared Lactobacillus plantarum fermentation liquid, and after centrifugation, discarding the supernatant, washing repeatedly with sterile water, and controlling the water content of the bacterial sludge at 30-35%, to obtain the Lactobacillus plantarum HP-ZW2 bacterial sludge.

[0010] By adopting the above technical solutions, the prepared Lactobacillus plantarum seed liquid is inoculated into a fermentation medium according to a certain ratio, the fermentation temperature and time are set, and after centrifugation and washing, the Lactobacillus plantarum bacterial sludge is obtained.

[0011] Optionally, the preparation method of the Lactobacillus plantarum seed liquid includes the following steps: inoculating the Lactobacillus plantarum slant strain into an MRS medium, and statically culturing at 37-40 °C for 12-15 h to obtain the Lactobacillus plantarum seed liquid.

[0012] By adopting the above technical solutions, the Lactobacillus plantarum slant strain is inoculated into the prepared MRS medium, and statically cultured in an environment of 37-40 °C to obtain the Lactobacillus plantarum seed liquid. During the whole process, contamination by miscellaneous bacteria is prevented, and the pH value, components and ratios of the medium are set to ensure the normal growth of Lactobacillus plantarum.

[0013] Optionally, Lactobacillus plantarum HP-ZW2 is deposited in the China Center for Type Culture Collection. The address of the depository is Wuhan University, Wuhan, China. The deposit number is CCTCC NO: M20221790, and the deposit date is November 14, 2022.

[0014] By adopting the above technical solution, Lactobacillus plantarum HP-ZW2 can decompose the organic matter in water, especially the residual bait, feces, etc. generated during the breeding process, thereby reducing the phenomenon of water eutrophication; degrade harmful substances such as ammonia nitrogen and nitrite in the water, reduce the organic oxygen consumption, and indirectly increase the oxygen content; inhibit the reproduction and growth of harmful bacteria in the water, thereby regulating the balance of bacteria and algae, preventing the outbreak of harmful bacteria in the water, and reducing the incidence of diseases of aquatic organisms.

[0015] Optionally, the preparation method of the modified activated carbon includes the following steps: (1) Disperse activated carbon powder in HNO3 solution, stir at room temperature for 30 - 35 min, wash with water, then disperse in an aqueous solution of silane coupling agent, stir for 50 - 55 min, filter and dry to obtain pretreated activated carbon; (2) Disperse the pretreated activated carbon obtained in step (1) in deionized water, add chitosan fiber, sorbitol, sodium dodecylbenzenesulfonate and nano silver, stir at a temperature of 55 - 60 °C for 1 - 2 h, and dry to obtain modified activated carbon.

[0016] By adopting the above technical solution, treating the activated carbon powder with HNO3 solution increases the number of functional groups on the surface of the activated carbon, such as carboxyl groups, hydroxyl groups, etc. These functional groups help to improve the adsorption capacity and selectivity of the activated carbon. The silane coupling agent forms a protective film on the surface of the activated carbon, improving the acid and alkali resistance and mechanical strength of the activated carbon.

[0017] Disperse the pretreated activated carbon in deionized water, and add chitosan fiber, sorbitol, sodium dodecylbenzenesulfonate and nano silver. The chitosan fiber can be loaded on the surface and pores of the activated carbon, increasing the adsorption capacity and selectivity of the activated carbon, especially having a better adsorption effect on polycyclic aromatic hydrocarbons, organochlorine pesticides, and heavy metal ions. Sorbitol, as a stabilizer or thickener, improves the stability and fluidity of the mixed system, enabling the chitosan fiber to be stably loaded in the activated carbon structure. The moisture retention and antioxidant properties of sorbitol also help to protect the adsorption performance of the activated carbon and chitosan fiber and extend their service life.

[0018] Sodium dodecylbenzenesulfonate, as a surfactant, can reduce the surface tension of the activated carbon surface, improve the dispersibility and stability of activated carbon and silver nanoparticles in water, reduce the probability of silver nanoparticle aggregation, and enable the stable loading of silver nanoparticles in the activated carbon structure. The addition of silver nanoparticles can endow the activated carbon with antibacterial properties, which helps to inhibit the growth and reproduction of bacteria in sewage. The stable loading of silver nanoparticles and chitosan fibers in the activated carbon structure not only increases the mechanical properties and adsorption properties of the activated carbon, but also helps to improve the efficiency of subsequent sewage treatment, and has good adsorption effects on polycyclic aromatic hydrocarbons, organochlorine pesticides, and heavy metal ions.

[0019] Optionally, the mass ratio of the activated carbon powder, chitosan fiber, and silver nanoparticles is 1:0.5 - 0.7:0.1 - 0.2.

[0020] By adopting the above technical solution, further limiting the mass ratio of the activated carbon powder, chitosan fiber, and silver nanoparticles within a certain range can improve the mechanical strength, adsorption property, and antibacterial property of the activated carbon. Due to its porous structure and large specific surface area, the activated carbon powder has extremely strong adsorption ability, effectively adsorbing pollutants such as organic matters, heavy metal ions, and pigments in aquaculture sewage, and reducing the chemical oxygen demand (COD) and biological oxygen demand (BOD) of the sewage.

[0021] Chitosan fibers contain functional groups such as amino and hydroxyl groups, and have selective adsorption ability for specific organic matters and heavy metal ions; they can be loaded on the surface of the activated carbon and act synergistically with the activated carbon powder to further improve the removal efficiency of pollutants. Silver nanoparticles have broad-spectrum antibacterial properties and can destroy the cell membranes and DNA of bacteria, thereby achieving the effect of sterilization. When combined with chitosan fibers and loaded on the surface of the activated carbon, they can inhibit the growth and reproduction of harmful bacteria, reduce the number of pathogenic bacteria in the water body, and reduce the risk of aquatic organisms getting sick.

[0022] The combined use of activated carbon powder, chitosan fiber, and silver nanoparticles can comprehensively improve water quality, remove suspended solids and pigments in water, increase the transparency of the water body, remove pollutants through adsorption, and inhibit the growth of harmful bacteria through antibacterial action, thereby maintaining the ecological balance of the water body.

[0023] Optionally, the preparation method of the modified ceramic particles includes the following steps: (1) Crush the ceramic particles into ceramic particle fine powder with a particle size of 100 - 200 μm, calcine at a temperature of 1200 - 1300 °C for 1 - 2 h, then disperse in deionized water, add sodium lignosulfonate, stir for 2 - 3 h, filter and dry to obtain pretreated ceramic powder; (2) Disperse the pretreated ceramic powder in deionized water, add borax, fatty alcohol polyoxyethylene ether sulfate, and xanthan gum, stir at a temperature of 35 - 40 °C for 2 - 3 h, and dry to obtain modified ceramic particles.

[0024] By adopting the above technical solution, the ceramic particles are crushed into fine powder to increase the surface area of the ceramic particles. The volatile components and organic matters in the ceramic powder are removed by calcination, and at the same time, its structure becomes more stable. Sodium lignosulfonate has good adsorption and dispersion properties. Adsorbing on the surface of the clay particles will generate electrostatic repulsion, causing the clay particles to disperse and hindering the agglomeration of the clay, thereby improving the fluidity of the ceramic slurry.

[0025] The pretreated ceramic powder is dispersed in deionized water again, and borax, fatty alcohol polyoxyethylene ether sulfate, and xanthan gum are added. Borax has good adsorption properties and chemical stability, and can efficiently adsorb and fix pollutants such as heavy metal ions in water, reducing their migration and release in water. Borax can be loaded in the ceramic particle structure and cooperate with the ceramic particles to increase the adsorption and mechanical strength of the ceramic particles. Fatty alcohol polyoxyethylene ether sulfate can improve the separation effect of suspended solids, precipitates, and oils in sewage, and promote the rapid precipitation and removal of suspended solids and precipitates. Moreover, fatty alcohol polyoxyethylene ether sulfate can significantly reduce the surface tension of water, making the ceramic particles easier to be wetted and dispersed by water.

[0026] Xanthan gum has a certain viscosity, which enables the ceramic particles and borax to bond with each other, making borax stably adhere to the structure of the ceramic particles and increasing the corresponding properties of the ceramic particles. Moreover, xanthan gum can also adsorb heavy metal ions and toxic and harmful substances in the wastewater, reducing the impact on the environment. The combined use of ceramic particles, borax, fatty alcohol polyoxyethylene ether sulfate, and xanthan gum can comprehensively improve the water quality. Through adsorption, biodegradation and other effects, pollutants in water are removed, and indicators such as chemical oxygen demand (COD) and biochemical oxygen demand (BOD) are reduced, improving the transparency and cleanliness of the water quality.

[0027] Optionally, the mass ratio of the ceramic particles, borax, and xanthan gum is 1:0.4 - 0.6:0.2 - 0.3.

[0028] By adopting the above technical solution, further limiting the mass ratio of ceramic particles, borax and xanthan gum within a certain range, the obtained modified ceramic particles have better adsorption properties and mechanical properties. Ceramic particles have a porous structure and a large specific surface area, and have good adsorption properties; borax has good adsorption properties and chemical stability, can efficiently adsorb and fix pollutants such as heavy metal ions in water, and reduce their migration and release in water. Xanthan gum has water absorption and gelation properties, can combine with pollutants in sewage to form large particle substances, which is convenient for later treatment and removal. Moreover, xanthan gum has a certain viscosity, which enables borax to adhere stably to the structure of ceramic particles, increasing the physical and chemical properties of ceramic particles. The combination of ceramic particles, borax and xanthan gum adsorbs pollutants such as suspended solids, organic matter and heavy metal ions in water, reduces the impact on the environment and purifies the water quality.

[0029] Optionally, the trace element salt is one or more of nickel chloride, copper sulfate, boric acid, sodium selenate, manganese chloride, sodium molybdate.

[0030] By adopting the above technical solution, the trace element salt can optimize the living environment of microorganisms, improve their activity, balance the nutrient structure in water, prevent the ecological system imbalance caused by insufficient or excessive nutrient salts, thereby enhancing the overall efficiency of the biological treatment system, enhancing the water purification ability, and making the effluent water quality cleaner and clearer.

[0031] In a second aspect, the present application provides a preparation method of a biological agent containing Lactobacillus plantarum HP-ZW2, comprising the following steps: mixing Lactobacillus plantarum HP-ZW2 bacterial sludge, starch, maltodextrin, modified activated carbon, skim milk powder, modified ceramic particles, trehalose, sucrose, trace element salt, and purified water, and stirring evenly to obtain a biological agent containing Lactobacillus plantarum HP-ZW2.

[0032] By adopting the above technical solution, the biological agent containing Lactobacillus plantarum HP-ZW2 can regulate the water quality, enhance the immunity of aquatic organisms, and play a comprehensive role in the sewage treatment after aquaculture. It not only adjusts the pH value of the water body, but also provides necessary nutritional support for microorganisms, promotes their growth and metabolic activities, and accelerates the degradation process of organic matter.

[0033] In summary, the present application has the following beneficial effects: 1. The present application provides Lactobacillus plantarum HP-ZW2 bacterial sludge as a beneficial microorganism, which can decompose organic matter in water, jointly maintain the ecological balance of the water body with other microorganisms in the water body, improve the water quality, and the modified activated carbon adsorbs organic matter, odors, pigments and heavy metal ions in water, improving the water quality.

[0034] 2. The present application provides modified ceramic particles as a biological carrier to provide an environment for the attachment and growth of microorganisms, promote the formation of biofilms, and enhance the biodegradation ability; trehalose has certain biocompatibility and stability in sewage treatment, which helps to maintain the activity of microorganisms.

[0035] 3. In the present application, the trace element salts provide the trace elements required for the activity of microbial enzymes and promote the growth and metabolism of microorganisms; when these components are mixed and used for the sewage treatment after aquaculture, they can play a synergistic role and improve the sewage treatment efficiency and water quality. Detailed implementation manners

[0036] The present application will be further described in detail below in conjunction with embodiments.

[0037] The raw materials used in the examples and comparative examples can all be obtained commercially.

[0038] Preparation examples of modified activated carbon Preparation example 1-1 The preparation method of modified activated carbon includes the following steps: (1) Disperse 50 kg of activated carbon powder in 70 L of HNO3 solution with a concentration of 4.0 mol / L, stir at room temperature for 35 min, wash with water, and then disperse it in 100 L of an aqueous solution of silane coupling agent KH-550 with a mass concentration of 0.3%, stir for 55 min, filter and dry to obtain pretreated activated carbon; (2) Disperse the pretreated activated carbon obtained in step (1) in 120 L of deionized water, add chitosan fiber, 10 kg of sorbitol, 5 kg of sodium dodecylbenzenesulfonate and nano silver, stir at a temperature of 60 °C for 2 h, and dry to obtain modified activated carbon.

[0039] The mass ratio of activated carbon powder, chitosan fiber and nano silver is 1:0.5:0.1.

[0040] Preparation example 1-2 The difference from Preparation example 1-1 is that in step (2), chitosan fiber is not added.

[0041] Preparation example 1-3 The difference from Preparation example 1-1 is that in step (2), nano silver is not added.

[0042] Preparation example 1-4 The difference from Preparation example 1-1 is that the mass ratio of activated carbon powder, chitosan fiber and nano silver is 1:0.7:0.2.

[0043] Preparation example 1-5 The difference from Preparation example 1-1 is that the mass ratio of activated carbon powder, chitosan fiber and nano silver is 1:0.1:0.8.

[0044] Preparation Examples of Modified Ceramic Particles Preparation Example 2-1 A method for preparing modified ceramic particles, comprising the following steps: (1) Crush 35 kg of ceramic particles into fine ceramic particle powder with a particle size of 150 μm, calcine at a temperature of 1250 °C for 2 h, then disperse in 55 L of deionized water, add 6 kg of sodium lignosulfonate, stir for 3 h, filter and dry to obtain pretreated ceramic powder; (2) Disperse the pretreated ceramic powder in 110 L of deionized water, add borax, 8 kg of sodium lauryl polyoxyethylene ether sulfate, and xanthan gum, stir at a temperature of 40 °C for 3 h, and dry to obtain modified ceramic particles.

[0045] The mass ratio of ceramic particles, borax and xanthan gum is 1:0.4:0.2.

[0046] Preparation Example 2-2 The difference from Preparation Example 2-1 is that in step (2), borax is not added.

[0047] Preparation Example 2-3 The difference from Preparation Example 2-1 is that in step (2), xanthan gum is not added.

[0048] Preparation Example 2-4 The difference from Preparation Example 2-1 is that the mass ratio of ceramic particles, borax and xanthan gum is 1:0.6:0.3.

[0049] Preparation Example 2-5 The difference from Preparation Example 2-1 is that the mass ratio of ceramic particles, borax and xanthan gum is 1:0.1:0.6. Examples

[0050] Example 1 A biological preparation containing Lactobacillus plantarum HP-ZW2, comprising the following raw materials by weight: 18 kg of Lactobacillus plantarum HP-ZW2 bacterial sludge, 55 kg of starch, 23 kg of maltodextrin, 29 kg of modified activated carbon, 12 kg of skim milk powder, 24 kg of modified ceramic particles, 8 kg of trehalose, 5 kg of sucrose, 8 kg of trace element salt, and 195 kg of purified water.

[0051] Preparation method of Lactobacillus plantarum HP-ZW2 bacterial sludge, comprising the following steps: inoculating the Lactobacillus plantarum seed liquid into the fermentation medium at an inoculation amount of 8%, fermenting in a 15L fermenter for 24h to obtain the Lactobacillus plantarum fermentation liquid, wherein the fermentation conditions are: temperature 35°C, liquid filling amount 65%, ventilation amount 0.1VVM, tank pressure 0.03MPa; then centrifuging the prepared Lactobacillus plantarum fermentation liquid, the centrifuging conditions are: 3500r / min, centrifuging for 10min, after centrifuging, discarding the supernatant, washing repeatedly with sterile water, and controlling the water content of the bacterial sludge at 32% to obtain the Lactobacillus plantarum HP-ZW2 bacterial sludge; wherein, the fermentation medium is a tomato powder medium, and the components are: beef extract 15g / L, tomato powder 5g / L, yeast extract 5g / L, lactose 20g / L, glucose 5g / L, dipotassium hydrogen phosphate 2g / L, sodium acetate anhydrous 15g / L, Tween-80 1g / L.

[0052] Inoculate the slant strain of Lactobacillus plantarum HP-ZW2 into the MRS medium with a volume of 180mL and a pH of 6.4 at an inoculation amount of 6%, and statically culture at 39°C for 14h to obtain the Lactobacillus plantarum seed liquid; wherein, the MRS medium: peptone 10.0g, beef extract 10.0g, yeast extract powder 5.0g, glucose 20.0g, Tween-80 1.0mL, K2HPO4·3H2O, sodium acetate anhydrous 5.0g, diammonium citrate 2.0g, MnSO4·H2O 0.058g, MgSO4·7H2O 0.29g, add the above components to distilled water, make up the volume to 1000mL, heat and dissolve, adjust the pH to 6.4, and sterilize at 121°C for 20min after dispensing.

[0053] Lactobacillus plantarum HP-ZW2 (Lactobacillus plantarum HP-ZW2) is preserved in the China Center for Type Culture Collection, the address of the preservation unit is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M20221790, and the preservation date is November 14, 2022; the trace element salt is nickel chloride.

[0054] The preparation method of the above-mentioned biological agent containing Lactobacillus plantarum HP-ZW2 comprises the following steps: mixing the Lactobacillus plantarum HP-ZW2 bacterial sludge, starch, maltodextrin, modified activated carbon, skimmed milk powder, modified ceramic particles, trehalose, sucrose, trace element salt, and purified water, and stirring evenly to obtain the biological agent containing Lactobacillus plantarum HP-ZW2.

[0055] The modified activated carbon is prepared by Preparation Example 1-1; the modified ceramic particles are prepared by Preparation Example 2-1.

[0056] Example 2 A biological preparation containing Lactobacillus plantarum HP-ZW2, which is different from Example 1 in that it contains the following raw materials by weight: 16 kg of Lactobacillus plantarum HP-ZW2 bacterial sludge, 50 kg of starch, 20 kg of maltodextrin, 27 kg of modified activated carbon, 10 kg of skim milk powder, 23 kg of modified ceramic particles, 10 kg of trehalose, 6 kg of sucrose, 7 kg of trace element salts, and 190 kg of purified water.

[0057] Example 3 A biological preparation containing Lactobacillus plantarum HP-ZW2, which is different from Example 1 in that it contains the following raw materials by weight: 20 kg of Lactobacillus plantarum HP-ZW2 bacterial sludge, 60 kg of starch, 25 kg of maltodextrin, 30 kg of modified activated carbon, 15 kg of skim milk powder, 26 kg of modified ceramic particles, 6 kg of trehalose, 3 kg of sucrose, 10 kg of trace element salts, and 200 kg of purified water.

[0058] Example 4 A concrete containing different mineral admixtures, which is different from Example 1 in that the modified activated carbon is prepared by Preparation Example 1-2.

[0059] Example 5 A concrete containing different mineral admixtures, which is different from Example 1 in that the modified activated carbon is prepared by Preparation Example 1-3.

[0060] Example 6 A concrete containing different mineral admixtures, which is different from Example 1 in that the modified activated carbon is prepared by Preparation Example 1-4.

[0061] Example 7 A concrete containing different mineral admixtures, which is different from Example 1 in that the modified activated carbon is prepared by Preparation Example 1-5.

[0062] Example 8 A concrete containing different mineral admixtures, which is different from Example 1 in that the modified ceramic particles are prepared by Preparation Example 2-2.

[0063] Example 9 A concrete containing different mineral admixtures, which is different from Example 1 in that the modified ceramic particles are prepared by Preparation Example 2-3.

[0064] Example 10 A concrete containing different mineral admixtures, which is different from Example 1 in that the modified ceramic particles are prepared by Preparation Example 2-4.

[0065] Example 11 A kind of concrete containing heterogeneous mineral admixtures, which is different from Example 1 in that the modified ceramic particles are prepared by Preparation Examples 2-5.

[0066] Comparative example Comparative Example 1 A kind of concrete containing heterogeneous mineral admixtures, which is different from Example 1 in that no modified activated carbon is added.

[0067] Comparative Example 2 A kind of concrete containing heterogeneous mineral admixtures, which is different from Example 1 in that the modified activated carbon is replaced with an equal amount of activated carbon.

[0068] Comparative Example 3 A kind of concrete containing heterogeneous mineral admixtures, which is different from Example 1 in that no modified ceramic particles are added.

[0069] Comparative Example 4 A kind of concrete containing heterogeneous mineral admixtures, which is different from Example 1 in that the modified ceramic particles are replaced with an equal amount of ceramic particles.

[0070] Performance detection test The biological agents containing Lactobacillus plantarum HP-ZW2 prepared in Examples 1-11 and Comparative Examples 1-4 were subjected to performance tests; the COD of the sewage was detected by the potassium dichromate method, the ammonia nitrogen of the sewage was detected by the flocculation precipitation Nessler reagent spectrophotometry, the total nitrogen of the sewage was detected by the potassium persulfate oxidation ultraviolet spectrophotometry, and the total phosphorus of the sewage was detected by the potassium persulfate oxidation ammonium molybdate spectrophotometry. The test results are shown in Table 1.

[0071] Table 1 Test data of examples and comparative examples Component COD / mg / L Ammonia nitrogen / mg / L Total nitrogen / mg / L Total phosphorus / mg / L Raw sewage 8.9 28.6 47.9 8.7 Example 1 0.8 0.5 4.6 0.6 Example 2 0.9 0.6 4.7 0.7 Example 3 1.0 0.7 4.8 0.8 Example 4 2.3 3.4 7.2 2.1 Example 5 1.7 2.5 6.1 1.5 Example 6 1.0 1.8 4.7 0.6 Example 7 1.4 2.1 5.3 1.1 Example 8 2.2 3.2 7.0 2.0 Example 9 1.6 2.4 6.0 1.4 Example 10 0.8 0.7 4.6 0.7 Example 11 1.3 2.0 5.2 1.0 Comparative example 1 5.5 8.4 15.6 5.3 Comparative example 2 3.7 6.2 11.7 3.5 Comparative example 3 5.3 8.1 15.3 5.0 Comparative example 4 3.2 5.4 11.1 3.0 As can be seen from Table 1, the biological agents containing Lactobacillus plantarum HP-ZW2 prepared in Examples 1-3 of the present application have good sewage treatment capabilities. Among them, the COD content of Example 1 is 0.8 mg / L, the ammonia nitrogen content is 0.5 mg / L, the total nitrogen content is 4.6 mg / L, and the total phosphorus content is 0.6 mg / L, which are significantly lower than the contents of each component in the original sewage, indicating that the prepared biological agents containing Lactobacillus plantarum HP-ZW2 have good sewage treatment capabilities. The modified activated carbon adsorbs organic matters, odors, pigments and heavy metal ions in the water, improves the water quality, and the modified ceramic particles serve as a biological carrier, providing an environment for the attachment and growth of microorganisms, promoting the formation of biofilms, and enhancing the biodegradation ability.

[0072] In Examples 4-5, the preparation methods of the modified activated carbon do not add chitosan fibers and nano silver respectively. In Examples 6-7, the mass ratios of activated carbon powder, chitosan fibers and nano silver are changed. As can be seen from Table 1, the COD content, ammonia nitrogen content, total nitrogen content and total phosphorus content in Examples 4-5 are significantly greater than those in Examples 1-3 and Example 6, while the detected contents of the corresponding components in Example 7 are greater than those in Examples 1-3 and Example 6, but less than those in Examples 4-5, indicating that chitosan fibers contain functional groups such as amino and hydroxyl groups and have selective adsorption ability for specific organic substances and heavy metal ions; they can be loaded on the surface of activated carbon and act synergistically with activated carbon powder to further improve the removal efficiency of pollutants. Nano silver has broad-spectrum antibacterial properties and can destroy the cell membranes and DNA of bacteria, thus achieving the effect of sterilization; it is combined with chitosan fibers and loaded on the surface of activated carbon to inhibit the growth and reproduction of harmful bacteria, reduce the number of pathogenic bacteria in water, and reduce the risk of aquatic organisms getting sick.

[0073] In Examples 8-9, the preparation methods of the modified ceramic particles do not add borax and xanthan gum respectively. In Examples 10-11, the mass ratios of ceramic particles, borax and xanthan gum are changed. As can be seen from Table 1, the COD content, ammonia nitrogen content, total nitrogen content and total phosphorus content in Examples 8-9 are significantly greater than those in Examples 1-3 and Example 10, while the detected contents of the corresponding components in Example 11 are greater than those in Examples 1-3 and Example 10, but less than those in Examples 8-9, indicating that ceramic particles have a porous structure and a large specific surface area and have good adsorption performance; borax has good adsorption performance and chemical stability and can efficiently adsorb and fix pollutants such as heavy metal ions in water, reducing their migration and release in water; xanthan gum has a certain viscosity, enabling borax to stably adhere to the structure of ceramic particles, increasing the physicochemical properties of ceramic particles. The combination of ceramic particles, borax and xanthan gum adsorbs pollutants such as suspended solids, organic substances and heavy metal ions in water, reduces the impact on the environment and purifies water quality.

[0074] In Comparative Example 1 and Comparative Example 3, the modified activated carbon and the modified ceramic particles are not added respectively. As can be seen from Table 1, compared with Example 1, the COD content, ammonia nitrogen content, total nitrogen content and total phosphorus content in Comparative Example 1 and Comparative Example 3 are significantly greater than those in Examples 1-2, indicating that the modified activated carbon adsorbs organic substances, odors, pigments and heavy metal ions in water, and the modified ceramic particles, as a biological carrier, provide an environment for the attachment and growth of microorganisms, promote the formation of biofilms and enhance the biodegradation ability.

[0075] In Comparative Example 2 and Comparative Example 4, the modified activated carbon was replaced with an equal amount of activated carbon, and the modified ceramic particles were replaced with an equal amount of ceramic particles. As can be seen from Table 1, the COD content, ammonia nitrogen content, total nitrogen content, and total phosphorus content of Comparative Example 2 and Comparative Example 4 are significantly greater than those of Examples 1-2, but less than those of Comparative Example 1 and Comparative Example 3, indicating that the modified activated carbon and modified ceramic particles prepared in this application have good adsorption performance and can adsorb organic matters, odors, pigments, and heavy metal ions in water, improving the sewage treatment efficiency and water quality.

[0076] This specific embodiment is only an interpretation of this application and does not limit this application. After reading this specification, those skilled in the art can make modifications to this embodiment without creative contributions as needed, but as long as they are within the scope of the claims of this application, they are protected by the patent law.

Claims

1. A biological preparation containing Lactobacillus plantarum HP-ZW2, characterized in that, It contains the following raw materials in parts by weight: 16 - 20 parts of Lactobacillus plantarum HP-ZW2 bacterial sludge, 50 - 60 parts of starch, 20 - 25 parts of maltodextrin, 27 - 30 parts of modified activated carbon, 10 - 15 parts of skim milk powder, 23 - 26 parts of modified ceramic particles, 6 - 10 parts of trehalose, 3 - 6 parts of sucrose, 7 - 10 parts of trace element salts, and 190 - 200 parts of purified water.

2. The biological agent containing Lactobacillus plantarum HP-ZW2 according to claim 1, characterized in that, The preparation method of the Lactobacillus plantarum HP-ZW2 bacterial sludge includes the following steps: inoculating the Lactobacillus plantarum seed liquid into a fermentation medium for fermentation to obtain a Lactobacillus plantarum fermentation liquid, centrifuging the prepared Lactobacillus plantarum fermentation liquid, discarding the supernatant after centrifugation, washing repeatedly with sterile water, and controlling the water content of the bacterial sludge at 30 - 35% to obtain the Lactobacillus plantarum HP-ZW2 bacterial sludge.

3. The biological agent containing Lactobacillus plantarum HP-ZW2 according to claim 2, characterized in that, The preparation method of the Lactobacillus plantarum seed liquid includes the following steps: inoculating the Lactobacillus plantarum slant strain into an MRS medium and statically culturing at 37 - 40 °C for 12 - 15 h to obtain the Lactobacillus plantarum seed liquid.

4. A biological preparation containing Lactobacillus plantarum HP-ZW2 according to claim 1, characterized in that, Lactobacillus plantarum HP-ZW (Lactobacillus plantarum HP-ZW2) is preserved in the China Center for Type Culture Collection. The address of the preservation unit is Wuhan University, Wuhan, China. The preservation number is CCTCC NO: M20221790, and the preservation date is November 14, 2022.

5. A biological preparation containing Lactobacillus plantarum HP-ZW2 according to claim 1, characterized in that, The preparation method of the modified activated carbon includes the following steps: (1) Disperse activated carbon powder in an HNO3 solution, stir at room temperature for 30 - 35 min, wash with water, then disperse in an aqueous solution of silane coupling agent, stir for 50 - 55 min, filter and dry to obtain pretreated activated carbon; (2) Disperse the pretreated activated carbon obtained in step (1) in deionized water, add chitosan fiber, sorbitol, sodium dodecylbenzenesulfonate, and nano silver, stir at a temperature of 55 - 60 °C for 1 - 2 h, and dry to obtain modified activated carbon.

6. A biological preparation containing Lactobacillus plantarum HP-ZW2 according to claim 5, characterized in that, The mass ratio of the activated carbon powder, chitosan fiber, and nano silver is 1:0.5 - 0.7:0.1 - 0.

2.

7. A biological preparation containing Lactobacillus plantarum HP-ZW2 according to claim 1, characterized in that The preparation method of the modified ceramic particles includes the following steps: (1) Crush ceramic particles into ceramic particle fine powder with a particle size of 100 - 200 μm, calcine at a temperature of 1200 - 1300 °C for 1 - 2 h, then disperse in deionized water, add sodium lignosulfonate, stir for 2 - 3 h, filter and dry to obtain pretreated ceramic powder; (2) Disperse the pretreated ceramic powder in deionized water, add borax, fatty alcohol polyoxyethylene ether sulfate, and xanthan gum, stir at a temperature of 35 - 40 °C for 2 - 3 h, and dry to obtain modified ceramic particles.

8. A biological preparation containing Lactobacillus plantarum HP-ZW2 according to claim 7, characterized in that, The mass ratio of the ceramic particles, borax, and xanthan gum is 1:0.4 - 0.6:0.2 - 0.

3.

9. A biological agent containing Lactobacillus plantarum HP-ZW2 according to claim 1, characterized in that, The trace element salts are one or more of nickel chloride, copper sulfate, boric acid, sodium selenate, manganese chloride, and sodium molybdate.

10. The preparation method of a biological preparation containing Lactobacillus plantarum HP-ZW2 according to claim 1, characterized in that, It includes the following steps: mixing the bacterial sludge of Lactobacillus plantarum HP-ZW2, starch, maltodextrin, modified activated carbon, skimmed milk powder, modified ceramic particles, trehalose, sucrose, trace element salts and purified water, and stirring evenly to obtain the biological preparation containing Lactobacillus plantarum HP-ZW2.