Novel biocontrol bacterium bacillus species, bacterium-pesticide compound agent and application of novel biocontrol bacterium bacillus species and bacterium-pesticide compound agent

Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a novel biocontrol bacterium bacillus species, a bacterium-pesticide mixture and application of the novel biocontrol bacterium bacillus species and the bacterium-pesticide mixture. The biocontrol bacterium bacillus new species is bacillus new species 122-9 and is preserved in China General Microbiological Culture Collection Center on April 1, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC No.34057, and the biocontrol bacterium bacillus new species is classified and named as bacillus sp. The living body inhibition rate of the strain 122-9 to branches with apple rot reaches 79%, the strain 122-9 has high compatibility with tebuconazole, carbendazim and pyraclostrobin, and after synergistic treatment of the strains and drugs, the inhibition rate to the apple rot also reaches 80% or above after the use of the three test drugs is halved.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganisms, and more specifically, to a new species of biocontrol bacterium Bacillus, a bacterium-pesticide compound agent and its application. Background Art

[0002] With the development of agriculture and intensive planting, plant diseases have become one of the main reasons restricting its production. For example, apple canker caused by Valsa mali, gray mold and ring rot diseases after fruit harvest, and root rot diseases caused by continuous cropping of plants, etc. have caused serious economic losses. Among them, apple canker occurs in the main apple producing areas of China and the world's apple producing areas. When the disease is severe, it can even invade the fruits and cause the diseased fruits to be reinfected, resulting in a 50%-70% reduction in orchard production and causing huge losses to the fruit industry economy. Chemical control is still the most direct and effective method for the prevention and control of forest and plant diseases. Nowadays, fungicides such as tebuconazole, difenoconazole, carbendazim, prochloraz, and pyraclostrobin have been registered for the prevention and control of apple canker, gray mold and ring rot diseases after pear fruit harvest. Chemical control has the advantages of high efficiency, quick effect, convenient use, and high economic benefits. However, the long-term improper use of chemical agents not only causes phytotoxicity to plants, causes poisoning of humans and livestock, kills beneficial microorganisms, but also can lead to the generation of drug resistance of pathogens. At the same time, the high residues of pesticides will also bring serious harm to human health and cause pollution to the ecological environment.

[0003] Biological control technology refers to the use of antagonistic microbial resources for the biological control of plant diseases, which has the advantages of rich strain resources, wide sources, safety for non-target organisms, small toxic and side effects, good environmental compatibility, and continuous action on harmful organisms. However, single biological control has the disadvantages of slow field control effect, unsatisfactory control effect, and being easily affected by the environment.

[0004] In recent years, the proposed bacterium-pesticide synergy technology combines biocontrol bacteria with low-dose chemical agents, which can not only exert the ecological friendly characteristics of biocontrol bacteria, but also utilize the rapid bacteriostatic effect of chemical agents, so as to achieve synergistic enhancement. This technology can not only effectively control diseases, but also reduce the usage amount of chemical agents, reduce the potential threats to the environment and human health, and provide new ideas for the green and sustainable development of the apple industry. However, there is less research and application on the bacterium-pesticide synergy of apple canker and other diseases at present. The actual application faces technical obstacles such as the lack of a scientific compatibility screening system, and the activity inhibition phenomenon exists in most potential bacterium-pesticide combinations. Based on the above statements, the present invention provides a new species of biocontrol bacterium Bacillus, a bacterium-pesticide compound agent and its application. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides a new species of biocontrol bacterium Bacillus, a bacterium-pesticide compound agent and its application.

[0006] In a first aspect, the present invention provides a new species of biocontrol bacterium Bacillus, adopting the following technical solution:

[0007] A new species of biocontrol bacterium Bacillus, the new species of biocontrol bacterium Bacillus being Bacillus sp. nov. 122-9, was deposited on April 1, 2025 at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC No. 34057 and classified and named as Bacillus sp.

[0008] Preferably, the 16S rRNA sequence of the Bacillus sp. nov. 122-9 is as shown in SEQ ID NO:1.

[0009] In a second aspect, the present invention provides the application of a new species of biocontrol bacterium Bacillus in the preparation of products against apple canker.

[0010] In a third aspect, the present invention provides the application of a new species of biocontrol bacterium Bacillus in the preparation of products for inhibiting plant pathogenic bacteria.

[0011] Preferably, the plant pathogenic bacteria include Botryosphaeria cinerea, Botryosphaeria berengeriana de, Monilinia fructigena, Fusarium solani WQ1, Phytophthora parasitica var. nicotianae, Rhizoctonia solani, and Fusarium pseudograminearum.

[0012] In a fourth aspect, the present invention provides a bactericide formulation, adopting the following technical solution:

[0013] A bactericide formulation includes the new species of biocontrol bacterium Bacillus described above.

[0014] Preferably, the active ingredients of the new species of biocontrol bacterium Bacillus include viable bacteria of Bacillus sp. nov. 122-9, fermentation broth or bacterial suspension.

[0015] Preferably, the bactericide formulation further includes tebuconazole, carbendazim or pyraclostrobin.

[0016] Preferably, the active ingredients of the bactericide formulation further include a tebuconazole dilution, a carbendazim dilution or a pyraclostrobin dilution.

[0017] Preferably, the bacterial and drug compounding agent comprises a Bacillus sp. nov. 122-9 bacterial suspension with an OD 600 = 0.8 and a 4000-fold dilution of tebuconazole in a volume ratio of 1:1.

[0018] Preferably, the bacterial and drug compounding agent comprises a Bacillus sp. nov. 122-9 bacterial suspension with an OD 600 = 0.8 and a 4000-fold dilution of carbendazim in a volume ratio of 1:1.

[0019] Preferably, the bacterial and drug compounding agent comprises a Bacillus sp. nov. 122-9 bacterial suspension with an OD 600 = 0.8 and a 4000-fold dilution of pyraclostrobin in a volume ratio of 1:1.

[0020] Preferably, the dosage form of the bacterial and drug compounding agent is a water dispersible granule, a water suspension concentrate or a dispersible oil suspension.

[0021] In summary, the present invention has the following beneficial effects:

[0022] In the present invention, soil samples were collected from the rhizosphere of Alhagi sparsifolia and Tamarix ramosissima in the Tarim Basin of Xinjiang, and a new strain of Bacillus, Bacillus sp. nov. 122-9, was screened therefrom. The in vivo inhibition rate of strain 122-9 against the branches with apple canker reached 79%. It has high compatibility with tebuconazole, carbendazim and pyraclostrobin. After the combined treatment of bacteria and drugs, it was found that the inhibition rate of the three tested drugs against apple canker also reached more than 80% when the dosage was halved, and there was no significant difference from the treatment effect of the original dosage of the tested drugs. Even when the dosage of the drugs was halved, the combined treatment with the 122-9 bacterial liquid slightly improved the prevention and control effect of apple canker, which not only reduced the pollution of chemical agents, but also improved the control effect, achieving the purpose of reducing drug use, maintaining efficacy and efficiently controlling apple canker.

[0023] Strain 122-9 of the present invention has a certain inhibitory effect on Botryosphaeria cinerea, Botryosphaeria berengeriana de, Monilinia fructigena, Fusarium solani WQ1, Phytophthora parasitica var. nicotianae, Rhizoctonia solani and Fusarium pseudograminearum, and the inhibition rate is above 30%. The inhibition rate against Botryosphaeria cinerea is 76.99%, and the inhibition rates against Botryosphaeria berengeriana de and Phytophthora parasitica var. nicotianae also reach above 60%, showing a broad antibacterial spectrum. Description of the Drawings

[0024] ​​​​​​Figure 1 It is the determination diagram of the inhibitory activity of strain 122-9 against Valsa mali QH2 in Example 1 of the present invention;

[0025] Figure 2 It is the determination diagram of the antibacterial spectrum of strain 122-9 in Example 2 of the present invention;

[0026] Figure 3 It is the in-dish culture characteristics and scanning electron microscope morphology diagram of strain 122-9 in Example 3 of the present invention;

[0027] Figure 4 It is the phylogenetic tree of strain 122-9 based on 16S rDNA sequence and whole genome in Example 3 of the present invention;

[0028] Figure 5 It is the determination diagram of the inhibitory activity of 5 tested medicaments against strain 122-9 by the Oxford cup method in Example 4 of the present invention;

[0029] Figure 6 It is the determination result diagram of the in-vivo control effect of the combination of bacteria and medicine against Valsa mali in Example 5 of the present invention. Detailed implementation manners

[0030] The present invention will be further elaborated in detail below in conjunction with the description drawings of the specification and specific embodiments. The embodiments are only used to explain the present invention and are not used to limit the scope of the present invention. The test methods used in the following embodiments are all conventional methods unless otherwise specified; the materials, reagents, etc. used are reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0031] The tested materials involved in the embodiments of the present invention are specifically as follows:

[0032] 1.1 Tested soil samples

[0033] Soil samples were collected from the rhizosphere of Alhagi sparsifolia and Tamarix ramosissima in the Tarim Basin of Xinjiang in July 2022 (E41°09′20″, N86°25′15″). After the collected soil was mixed evenly, it was sealed and stored in a kraft paper bag, numbered, and placed in a refrigerator at 4°C in the Laboratory of Pathogens and Disease Control of Horticultural Crops, Inner Mongolia Agricultural University for standby.

[0034] 1.2 Tested strains

[0035] Botryosphaeria cinerea, Botryosphaeria berengeriana de, and Monilinia fructigena were isolated and identified by the Institute of Pomology, Chinese Academy of Agricultural Sciences (Sun et al. 2017; Sun Pingping et al., 2018). Cytospora mali QH2 was isolated and identified by our laboratory (Ma Qiang et al., 2020). Fusarium solani WQ1 was provided by the Institute of Vegetables and Flowers, Inner Mongolia Academy of Agricultural Sciences (Han Fengying et al., 2020). Phytophthora parasitica var. nicotianae, Rhizoctonia solani, and Fusarium pseudograminearum were provided by Henan Agricultural University.

[0036] 1.3 Test media:

[0037] (1) Potato Dextrose Agar (PDA): 200 g of potato extract, 20 g of dextrose, 20 g of agar powder, 1000 mL of distilled water, pH 7.2 - 7.4.

[0038] (2) LBA medium: 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, 20 g of agar powder, made up to 1 L with deionized water.

[0039] (3) LB liquid medium: same as LBA medium, without agar powder.

[0040] 1.4 Test agents

[0041] The test agents were purchased from the local market. Information such as the name of the agent, content of the active ingredient, dosage form, and manufacturer is shown in Table 1 below.

[0042] Table 1 Five test agents

[0043]

[0044] Example 1

[0045] Isolation and screening of antagonistic strains

[0046] Strains from the test soil samples were isolated using the serial dilution method, and single colonies were picked and transferred to LBA medium for antagonistic activity screening. The plate confrontation method and the in vitro shoot inoculation method were used to screen and isolate the strains.

[0047] The antagonistic strains were screened by the plate confrontation method. Pick and culture the mycelial disc of the apple canker pathogen QH2 and place it in the center of a PDA plate. Inoculate the isolated strains in two directions, 3 cm to the left and right of the mycelial disc of the apple canker pathogen QH2, and incubate at a constant temperature of 25 °C. Use the plate inoculated only with the apple canker pathogen QH2 as the control. When the control colony covers the plate, observe and record the colony radius. Select the strains with better antibacterial effect and stable antagonistic activity as candidate strains for the in vitro twig inoculation experiment.

[0048] The antagonistic activity of the selected candidate strains against apple canker on Jinhong twigs was determined by the in vitro twig inoculation method. Pick the candidate strains and inoculate them into 200 mL of LB liquid medium, and culture at 28 °C and 180 r / min for 48 h. Adjust the OD 600 = 0.8 to obtain the bacterial liquid for use. Select two-year-old healthy Jinhong apple twigs, cut them into small twigs about 10 cm long, disinfect them with sodium hypochlorite and alcohol respectively, rinse them with sterile water and then air-dry them. Seal both ends of the twigs with paraffin. Use a sterile puncher to heat-sterilize and make a 6-mm wound behind the branching part in the middle of the apple twig. After spraying the bacterial liquid on the apple twig, attach the mycelial disc of the apple canker pathogen QH2 to the wound site, wrap it with sterile absorbent cotton moistened with sterile water, and then wrap it with plastic wrap. Use the treatment of spraying LB liquid medium after wounding and then inoculating QH2 as the positive control (CK), and use the twigs sprayed only with LB liquid medium without inoculating the mycelial disc of the apple canker pathogen as the negative control. All twigs were placed in a moisturizing incubator at 25 °C with a 16-h light / 8-h dark cycle. After 7 days of inoculation, remove the absorbent cotton and re-inoculate the mycelial disc of the apple canker pathogen QH2 and then directly wrap it with plastic wrap. After 14 days, measure the size of the lesion (longitudinal length) and calculate the inhibition rate. The results are shown in Table 2 and Figure 1 .

[0049] Table 2 Determination results of the antagonistic activities of strain 122-9 against the apple canker pathogen QH2 in the plate and on in vitro twigs

[0050]

[0051] Combined with Table 2 and Figure 1 the displayed results show that: A total of 25 bacteria were isolated and purified from the rhizosphere soil samples of Alhagi sparsifolia and Tamarix ramosissima. The active antagonistic bacterium 122-9 was screened by the plate confrontation method and the in vitro twig method. Its inhibition zone against the apple canker pathogen QH2 in the plate was 2.41 mm, and the inhibition rate was 56.35%. In the in vitro twig assay, the inhibition rate against the apple canker pathogen QH2 reached 77.74%. In Table 2, different letters in the same column indicate significant differences between the two treatments at the P < 0.05 level (lesion size).

[0052] Example 2

[0053] Determination of the antibacterial spectrum of the strain

[0054] The antibacterial spectrum of the isolated antagonistic bacterium 122-9 was determined by the plate confrontation method. Different pathogen disks with a diameter of 6 mm after 5 days of cultivation were picked and placed in the center of a PDA plate. Antagonistic bacterium 122-9 disks that had grown on the medium for 3 days were inoculated in two directions, 3 cm to the left and right of the pathogen disk, and then incubated at a constant temperature of 25°C. Each treatment was repeated 3 times, and the petri dish inoculated only with the pathogen was used as the control (CK). When the colonies in the control group covered the plate, the diameter of the inhibition zone was observed and recorded. The results are shown in Table 3 and Figure 2 .

[0055] Average inhibition rate (%) = [1 - (average radius of the pathogen in the treatment group) / (average radius of the pathogen in the control group)] × 100%.

[0056] Table 3 Results of the determination of the antibacterial spectrum of strain 122-9

[0057]

[0058] Combined with Table 3 and Figure 2 the displayed results show that: Strain 122-9 has a certain inhibitory effect on different tested pathogens, with an inhibition rate above 30%. Its inhibition rate against Botrytis cinerea of pear is 76.99%, and the inhibition rates against Physalospora piricola and Phytophthora parasitica of tobacco also reach above 60%, indicating a broad antibacterial spectrum.

[0059] Example 3

[0060] Strain identification

[0061] (1) Morphological identification

[0062] The purified strain 122-9 was inoculated into LBA medium, and the colony morphological characteristics in the petri dish were observed. Colonies were picked and the cell morphology was observed by scanning electron microscopy. Strain 122-9 had typical bacterial colony characteristics on LBA medium. The colonies were milky white to light yellow opaque colonies, with a raised surface, an uneven edge, and a fast growth rate. Microscopic observation showed that the cells were short rod-shaped, approximately 14 - 15 μm × 5 - 6 μm in size. Figure 3 Among them, the left side is the front colony characteristic diagram of strain 122-9 on LBA medium, the middle is the back colony characteristic diagram of strain 122-9 on LBA medium, and the right side is the result diagram of the cell morphology of strain 122-9 observed by scanning electron microscopy.

[0063] (2) Molecular identification

[0064] Extract the genomic DNA of strain 122-9, perform whole-genome sequencing on it using Pacbio Sequel II, and use SMARTLink 10.1.0 software to assemble the reads obtained from the sequencing. Calculate the evolutionary distance between the genomes of different strains using the genomic alignment distance evolution method in the online program of Type Strain Genome Server (https: / / tygs.dsmz.de). Using the minimum evolutionary distance between the obtained genomes, use FASTME 2.1.6.1 software to construct the phylogenetic evolutionary trees of the 16S rRNA and whole-genome sequences of the strains screened in this experiment and these 12 strains (Meier-Kolthoff & Göker, 2019). The numbers on each branch represent the confidence rate of 1000 bootstrap tests. Calculate the average nucleotide identity (ANI) of the whole-genome sequences of 122-9 and related strains using Jspecies (Goris et al., 2007). The results are shown in Table 4 below and Figure 4 。

[0065] Table 4 ANI values of related strains and 122-9

[0066]

[0067] Combined with Table 4 and Figure 4 The displayed results show that: the ANI value of strain 122-9 and Bacillus vallismorti DV1-F-3 is 93.42%, the ANI value with Bacillus inaquosorum KCTC13429 is 93.08%, and the ANI values with other strains are between 76.07% and 92.88%. Since a whole-genome sequence nucleic acid identity of over 95-96% is considered the same species (Meier-Kolthoff et al., 2013). Combining the phylogenetic evolutionary tree, strain 122-9 and related species belong to different species or subspecies in the evolutionary tree. Therefore, based on the phylogenetic evolutionary tree, 122-9 is determined to be a new species of Bacillus, named Bacillus sp. 122-9. Figure 4 In it, A represents the phylogenetic evolutionary tree constructed based on 16S rRNA, B represents the phylogenetic evolutionary tree constructed based on the whole genome, and different colors in species cluster and subspecies cluster represent different species or subspecies.

[0068] The 16S rRNA sequence of the new species of Bacillus 122-9 is shown in SEQ ID NO:1:

[0069]

[0070] The newly discovered Bacillus sp. 122-9 of the present invention was deposited at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on April 1, 2025. The address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No. 34057, and the taxonomic name is Bacillus sp.

[0071] Example 4

[0072] Determination of fungicide compatibility

[0073] Five tested fungicides, namely tebuconazole, prochloraz, carbendazim, difenoconazole and pyraclostrobin, which have good control effects on apple canker, were selected to detect the inhibitory activity of the above five tested fungicides against strain 122-9 by the Oxford cup method. The specific method is as follows: First, dilute each tested fungicide to 4000 times the recommended concentration for standby. Add strain 122-9 to LBA medium and culture it at 28°C and 180 r / min for 48 h to obtain 122-9 bacterial liquid. Absorb 5 mL of 122-9 bacterial liquid and add it to 200 mL of melted but not solidified LBA medium, shake well and pour it into a petri dish to make a bacteria-containing plate. After the petri dish solidifies, place Oxford cups, add 100 μL of the diluted tested fungicide to each Oxford cup, repeat each treatment 3 times, and after culturing at 28°C for 2 d, measure the diameter of the clear zone of each colony.

[0074] Combined with Figure 5 The results show that tebuconazole, carbendazim and pyraclostrobin have good compatibility with strain B122-9. The clear zone radii of tebuconazole, carbendazim and pyraclostrobin against strain B122-9 are 0.465, 0.40 mm and 0.4 mm respectively, while the diameters of the inhibition zones of the fungicides prochloraz and difenoconazole against 122-9 are greater than 1 mm, which are 1.645 mm and 1.545 mm respectively. Therefore, three fungicides with good compatibility, tebuconazole, carbendazim and pyraclostrobin, were selected for compounding with strain 122-9.

[0075] Example 5

[0076] Control effect of the combination of fungicide and bacteria on apple canker

[0077] The control effect of the tested fungicides tebuconazole, carbendazim and pyraclostrobin compounded with strain 122-9 on apple canker was evaluated by the in vitro twig assay. The specific method is as follows: Dilute the tested fungicides tebuconazole, carbendazim and pyraclostrobin to 4000-fold solutions according to their recommended concentrations for standby. Pick strain 122-9 and inoculate it into 200 mL of LB liquid medium, culture it at 28°C and 180 r / min for 48 h, and adjust the OD with sterile water 600=0.8 to obtain the bacterial liquid of 122-9 for later use. Set CK- (not inoculated with pathogenic bacteria after LB treatment), CK+ (LB + pathogenic bacteria), the bacterial liquid of 122-9, the 4000-fold dilution of carbendazim, the 4000-fold dilution of pyraclostrobin, the 4000-fold dilution of tebuconazole, the 4000-fold dilution of carbendazim + the bacterial liquid of 122-9 with a volume ratio of 1:1, the 4000-fold dilution of pyraclostrobin + the bacterial liquid of 122-9 with a volume ratio of 1:1, the 4000-fold dilution of tebuconazole + the bacterial liquid of 122-9 with a volume ratio of 1:1; a total of 9 groups of treatment liquids.

[0078] Select two-year-old healthy Golden Red apple branches, cut them into small branches about 10 cm long, disinfect them with sodium hypochlorite and alcohol respectively, rinse them with sterile water and then air-dry them, seal both ends of the branches with paraffin, and use a sterile punch to heat-sterilize and make a 6-mm wound behind the middle branch part of the apple branch. After spraying the apple branches with 9 groups of treatment liquids for 30 min respectively, except for the CK- group without inoculating pathogenic bacteria, for the remaining 8 groups, apply the apple rot pathogen QH2 bacterial cake to the wound site, wrap it with sterile absorbent cotton moistened with sterile water, and then wrap it with plastic wrap. Place all the branches in a moisturizing culture at 25 °C with 16 h of light / 8 h of darkness. After 7 days of inoculation, remove the absorbent cotton and re-inoculate the apple rot pathogen QH2 bacterial cake and then directly wrap it with plastic wrap. After 14 days, measure the size of the lesion (longitudinal length), calculate the inhibition rate, and the results are shown in Table 5 below and Figure 6 .

[0079] Table 5 Control effect of the combination of bacteria and drugs on apple rot

[0080]

[0081] Combined with Table 5 and Figure 6 The displayed results show that: the in vivo inhibition rate of the bacterial liquid of strain 122-9 against apple rot branches reached 79%, showing certain inhibitory activity. After using the combination of bacteria and drugs, it was found that the inhibition rate of the three tested drugs against apple rot also reached more than 80% when the dosage was halved, and there was no significant difference from the treatment effect of the original dosage of the tested drugs. Even when the dosage of the drugs was halved and compounded with the bacterial liquid of 122-9, the control effect on apple rot was slightly improved. The bacteria-drug compound agent proposed by the present invention not only reduces the pollution of chemical agents but also improves the control effect, achieving the purpose of reducing drugs, maintaining efficacy, and efficiently controlling apple rot.

[0082] This specific embodiment is only an explanation of the present invention and is not a limitation thereof. Those skilled in the art can make modifications without creative contributions to this embodiment according to needs after reading this specification, but as long as it is within the scope of the claims of the present invention, it is protected by the patent law.

Claims

1. A new species of biocontrol bacterium Bacillus, characterized in that, The new biocontrol bacterium Bacillus species is Bacillus species 122-9, which was deposited on April 1, 2025 at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC No. 34057 and the taxonomic name Bacillus sp.

2. The new species of biocontrol bacterium Bacillus according to claim 1, characterized in that, The 16S rRNA sequence of the new Bacillus species 122-9 is shown in SEQ ID NO:

1.

3. Application of the new biocontrol bacterium Bacillus species according to claim 1 or 2 in the preparation of a product against apple rot.

4. Application of the new biocontrol bacterium Bacillus species according to claim 1 or 2 in the preparation of a product for inhibiting plant pathogenic bacteria.

5. Use of the new species of biocontrol bacterium Bacillus according to claim 4 in the preparation of a product for inhibiting plant pathogenic bacteria, characterized in that, The plant pathogenic bacteria include Botryosphaeria cinerea, Botryosphaeria berengerianade, Monilinia fructigena, Fusarium solani WQ1, Phytophthora parasitica var. nicotianae, Rhizoctonia solani, and Fusarium pseudograminearum.

6. A bacterial medicine compounding agent, characterized in that, It includes the new biocontrol bacterium Bacillus species according to claim 1 or 2.

7. The bacterial medicine compounding agent according to claim 6, wherein The active ingredients of the bactericide compound preparation include viable bacteria, fermentation broth or bacterial suspension of Bacillus species 122-9.

8. The microbial agent compounding agent according to claim 6, wherein The bactericide compound preparation also includes tebuconazole, carbendazim or pyraclostrobin.

9. The bacterial medicine compounding agent according to claim 8, characterized in that, The active ingredients of the bactericide compound preparation also include diluted tebuconazole solution, diluted carbendazim solution or diluted pyraclostrobin solution.

10. The bacterial medicine compounding agent according to claim 6, wherein, The dosage form of the bactericide compound preparation is a water dispersible granule, a water suspension concentrate or a dispersible oil suspension.

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