Primer and probe combination for subspecies typing identification of mycobacterium abscessus as well as method and application of primer and probe combination
Through fluorescent PCR technology combining specific primers and fluorescent probes, the time-consuming and complexity of the identification of Mycobacterium abscess subspecies in the prior art was solved, and rapid and accurate subspecies typing identification was achieved, which was suitable for conventional clinical microbial laboratory applications.
CN120400384APending Publication Date: 2025-08-01HANGZHOU RED CROSS HOSPITAL (ZHEJIANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE & WESTERN MEDICINE)
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Patent Information
- Application Number
- CN202510700303.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-28
- Publication Date
- 2025-08-01
AI Technical Summary
Technical Problem
The existing mycobacterium identification methods are time-consuming, complex and expensive, making it difficult to quickly and accurately distinguish the three subspecies of Mycobacterium abscess, affecting the guidance of antibiotic use.
Method used
The combination of specific primers and fluorescent probes and combined with fluorescent PCR technology, subspecies typing and identification were performed directly from clinical samples, and multi-objective detection was achieved through multiple fluorescent labeling.
Benefits of technology
It achieves rapid and accurate subspecies typing, shortens the diagnosis cycle, reduces the complexity of laboratory operations, and improves detection throughput and economy.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention relates to the technical field of typing identification, in particular to a primer and probe combination for typing identification of mycobacterium abscessus subspecies and a method and application of the primer and probe combination. The primer and probe combination comprises three subspecies specific amplification primer pairs and three subspecies specific TaqMan fluorescent probes; the sequences of the three pairs of subspecies specific amplification primers are SEQ ID NO. 1 to SEQ ID NO. 6; wherein the TaqMan fluorescent probe comprises three TaqMan fluorescent probes corresponding to mycobacterium abscessus subtypes, two ends of each TaqMan fluorescent probe are respectively provided with a fluorophore and a quenching group, and each sequence is SEQ ID NO.7 to SEQ ID NO.9. The invention further discloses a kit with the PCR reaction liquid, and by means of the PCR reaction liquid, the mycobacterium abscessus can be rapidly and accurately detected and identified, and subspecies typing identification can be conducted on the mycobacterium abscessus.
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