Aluminum ion composite nanofiber with antibacterial property as well as preparation method and application of aluminum ion composite nanofiber
By preparing aluminum ion composite nanofibers, the problems of frequent replacement of traditional dressings and insufficient therapeutic characteristics are solved, and the effect of efficient inhibition of bacterial growth and promoting wound repair is achieved.
Patent Information
- Application Number
- CN202510461272.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-14
- Publication Date
- 2025-08-01
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Traditional wound dressings require frequent replacement and lack a variety of therapeutic properties, such as good histocompatibility, biodegradation rate, antibacterial activity and ability to promote cell regeneration, making it difficult to effectively treat chronic wounds.
Aluminum ion composite nanofibers with antibacterial properties are prepared, and nanofibers with PDA coating are formed by co-assembling aluminum ions and nucleic acid segments, and wound repair is promoted using photothermal conversion and antibacterial properties.
It has achieved efficient inhibition of bacterial growth, good biocompatibility and photothermal conversion effect, and promoted wound repair.
Smart Images
Figure CN120401233A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of nanomaterials, and particularly to an aluminum ion composite nanofiber with antibacterial properties, its preparation method and application. Background Art
[0002] The skin is one of the body's barriers most vulnerable to external factors. The structural function of the skin can be damaged due to various stresses, resulting in wounds. Generally, wounds are classified into two categories, acute and chronic, according to the length of the healing time. Acute wounds follow the normal tissue repair process and can recover within a few days to a few weeks. Chronic wounds, however, are hindered by the repair sequence and time, leading to stalled healing or even deterioration. Therefore, treating chronic wounds faster and better is a major challenge.
[0003] Wound dressings play a crucial role in hemostasis, infection control, and wound healing. However, traditional clinical dressings, such as gauze and cotton pads, need to be changed frequently. An ideal wound dressing should have advantages such as good tissue compatibility and non-toxicity, a biodegradation rate matching the wound healing process, appropriate antibacterial activity to prevent infection, and bioactivity to promote cell and tissue regeneration. Therefore, developing innovative biomaterials with multiple therapeutic properties as ideal wound dressings remains an urgent need at present.
[0004] With the improvement of people's living standards and awareness of the health environment, the production of nano antibacterial materials and their products will become an important emerging industry. The core component of nano antibacterial materials is the antibacterial agent. Antibacterial agents are chemical components highly sensitive to some microorganisms such as bacteria and molds. A very small amount of antibacterial agent added to ordinary materials can make antibacterial materials. Therefore, many nanomaterials show great potential in antibacterial and thus promoting wound healing. Excellent drug-loading capacity and self-healing properties enable nanomaterials not only to deliver drugs efficiently but also to have synergistic therapeutic effects such as active ion release, catalytic biomimetic enzymes, photothermal and photodynamic therapies to promote wound repair.
[0005] In view of the characteristics of chronic wounds, nanomaterials can also respond to specific stimuli (including endogenous stimuli such as glucose, enzymes, ROS, and pH value, and exogenous stimuli such as light, magnetic field, and temperature), thus achieving more precise therapeutic effects. These stimulus-responsive nano systems have been widely studied and applied in wound treatment. The present application aims to study a new type of antibacterial drug delivery system that loads nanofiber antibacterial agents for promoting skin repair, hoping to provide a more efficient wound treatment plan. Summary of the Invention
[0006] This application provides an aluminum ion composite nanofiber with antibacterial properties. The prepared aluminum ion composite nanofiber loaded with antibacterial properties has good biocompatibility, photothermal conversion effect, and antibacterial characteristics, and can be used to effectively inhibit bacterial growth and promote the effect of wound repair treatment.
[0007] To solve the above technical problems, in a first aspect, an embodiment of this application provides an aluminum ion composite nanofiber with antibacterial properties, including the following steps: First, mix AlCl3 and polyA 20 and heat to obtain Al-polyA 20 nanofibers; then, dissolve DA in Tris buffer solution to obtain a DA solution; finally, disperse the Al-polyA 20 nanofibers into the DA solution, and after mixing, successively perform dark stirring, centrifugation, and washing to obtain Al-polyA 20 @PDA nanofibers.
[0008] In some exemplary embodiments, mixing AlCl3 and adenine polyA 20 and heating to obtain Al-polyA 20 nanofibers includes: dissolving AlCl3 and polyA 20 respectively in a solvent to obtain an AlCl3 solution and a polyA 20 solution; mixing and heating the AlCl3 solution and the polyA 20 solution to obtain Al-polyA20 nanofibers.
[0009] In some exemplary embodiments, the solvent is a Tris buffer solution containing 10 mM magnesium ions, with a pH of 7-9; the reaction concentration of the AlCl3 solution is 10-100 mM; the reaction concentration of the polyA 20 solution is 10-80 μM.
[0010] In some exemplary embodiments, the heating temperature is 90-100 °C, and the reaction time is 1-3 h.
[0011] In some exemplary embodiments, the AlCl3 solution and the polyA 20 solution are mixed at a molar ratio of 900:1-1100:1.
[0012] In some exemplary embodiments, the concentration of the DA solution is 0.5 mg / mL.
[0013] In some exemplary embodiments, the dispersion concentration of the Al-polyA 20 nanofibers is 10-100 nM aluminum ion equivalent concentration.
[0014] In some exemplary embodiments, Al-polyA 20 nanofibers are dispersed into the DA solution, and the stirring is carried out in the dark for 2 to 4 h; centrifugation is performed with ultrapure water, the rotation speed of centrifugation is 10,000 to 14,000 rpm, the centrifugation time is 10 to 15 min, and the number of washing times is 3 to 4 times.
[0015] In a second aspect, an embodiment of the present application further provides an aluminum ion composite nanofiber with antibacterial properties, which is prepared by using the preparation method of the aluminum ion composite nanofiber with antibacterial properties described in the above embodiment.
[0016] In some exemplary embodiments, the aluminum ion composite nanofiber has the following characteristics: the surface charge is -22.8 mV; the photothermal conversion condition is: irradiated with 808 nm near-infrared light with a power of 0.5 to 0.9 W / cm 2 for 1 to 5 min, and the temperature rise is 0 to 35 °C; the antibacterial condition is: the sample concentration is 0 to 20 mM, and irradiated with 808 nm near-infrared light with a power of 0.6 W / cm 2 for 2 to 4 min, and the minimum inhibitory concentration in Staphylococcus aureus is 8 mM.
[0017] In a third aspect, an embodiment of the present application further provides an application of the aluminum ion composite nanofiber with antibacterial properties described in the above embodiment in antibacterial drug preparations.
[0018] The technical solution provided by the embodiment of the present application has at least the following advantages:
[0019] The present application provides an aluminum ion composite nanofiber with antibacterial properties, a preparation method and an application thereof. The preparation method includes the following steps: First, AlCl3 and polyA 20 are mixed and heated to obtain Al-polyA 20 nanofibers; then, DA is dissolved in Tris buffer solution to obtain a DA solution; finally, the Al-polyA 20 nanofibers are dispersed into the DA solution, and after mixing, they are successively subjected to stirring in the dark, centrifugation, and washing to obtain Al-polyA 20 @PDA nanofibers.
[0020] The present application prepares a metal-nucleic acid composite nanofiber through the co-assembly of aluminum ions and nucleic acid segments, and then prepares a composite nanofiber with a PDA coating through in-situ polymerization to prepare a wound-targeted composite nanofiber antibacterial agent. The prepared aluminum ion composite nanofiber loaded with antibacterial properties has good biocompatibility, photothermal conversion effect and antibacterial characteristics, and can be used to efficiently inhibit the growth of bacteria and promote the effect of wound repair treatment. Description of the Drawings
[0021] One or more embodiments are illustrated by way of example in the accompanying drawings, which do not constitute a limitation on the embodiments unless otherwise stated. The figures in the accompanying drawings do not constitute a scale limitation.
[0022] Figure 1 Transmission electron microscopy image of the aluminum ion composite nanofibers prepared in the embodiments of the present application.
[0023] Figure 2 Zeta potential map of the aluminum ion composite nanofibers prepared in the embodiments of the present application.
[0024] Figure 3A Schematic diagram of the photothermal properties of Al-polyA 20 @PDA nanofibers at different powers.
[0025] Figure 3B Schematic diagram of the photothermal properties of Al-polyA 20 @PDA nanofibers at different concentrations.
[0026] Figure 3C Schematic diagram of the photothermal stability of Al-polyA 20 @PDA nanofibers.
[0027] Figure 4A Colony images of different concentrations of Al-polyA 20 @PDA nanofiber materials incubating Staphylococcus aureus with and without near-infrared irradiation.
[0028] Figure 4B Quantitative data diagrams of different concentrations of Al-polyA 20 @PDA nanofiber materials incubating Staphylococcus aureus with and without near-infrared irradiation.
[0029] Figure 5A Colony images of different concentrations of Al-polyA 20 @PDA nanofiber materials incubating Escherichia coli with and without near-infrared irradiation.
[0030] Figure 5B Quantitative data diagrams of different concentrations of Al-polyA 20 @PDA nanofiber materials incubating Escherichia coli with and without near-infrared irradiation.
[0031] Figure 6Al-polyA prepared for the embodiments of the present application 20 Schematic diagram of the cytotoxicity of @PDA nanofiber material against L929 cells. Detailed implementation manners
[0032] As can be seen from the background art, traditional clinical dressings need to be changed frequently. Currently, there is an urgent need to develop innovative biomaterials with multiple therapeutic properties as ideal wound dressings.
[0033] The present application aims to study a new type of antibacterial drug delivery system that loads nanofiber antibacterial agents for promoting skin repair, and expects it to provide a more efficient wound treatment plan. The embodiments of the present application provide an aluminum ion composite nanofiber with antibacterial properties, its preparation method and application. The preparation method includes the following steps: First, mix AlCl3 and polyA 20 and heat to obtain Al-polyA 20 nanofibers; then, dissolve DA in Tris buffer solution to obtain a DA solution; finally, disperse the Al-polyA 20 nanofibers into the DA solution, and after mixing, carry out light-shielding stirring, centrifugation, and washing in sequence to obtain Al-polyA 20 @PDA nanofibers. The present application provides an aluminum ion composite nanofiber with antibacterial properties. The prepared aluminum ion composite nanofiber loaded with antibacterial properties has good biocompatibility, photothermal conversion effect, and antibacterial characteristics, and can be used to efficiently inhibit bacterial growth and promote the effect of wound repair treatment.
[0034] The following will elaborate on the embodiments of the present application with reference to the accompanying drawings. However, those of ordinary skill in the art can understand that in the embodiments of the present application, many technical details are proposed to help readers better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical solutions claimed in the present application can still be implemented.
[0035] The embodiments of the present application provide a preparation method for an aluminum ion composite nanofiber with antibacterial properties, including the following steps:
[0036] Step 1: Mix AlCl3 and polyA 20 and heat to obtain Al-polyA 20 nanofibers.
[0037] Step 2: Dissolve DA in Tris buffer solution to obtain a DA solution.
[0038] Step 3: Disperse the Al-polyA 20The nanofibers are dispersed into the DA solution. After mixing, they are successively subjected to light-shielding stirring, centrifugation, and washing to obtain Al-polyA 20 @PDA nanofibers.
[0039] Figure 1 This is the transmission electron microscopy image of the aluminum ion composite nanofibers prepared in the examples of this application. In this application, adenine oligonucleotide (polyA 20 ) is used as the raw material. Based on the DNA assembly technology driven by coordination and electrostatic interactions, it is co-assembled with trivalent aluminum ions (Al(Ⅲ)) to prepare Al-polyA 20 nanofibers; subsequently, dopamine (DA) is in-situ polymerized on its surface to coat a polydopamine (PDA) coating to prepare Al-polyA 20 @PDA nanofibers.
[0040] The aluminum ion composite nanofiber antibacterial agent prepared in this application, the inner layer of Al-polyA 20 nanofiber morphology can break through the bacterial cell membrane, cause oxidative damage, and hinder bacterial metabolism, thus bringing antibacterial effects and having good antibacterial properties; the outer layer of coated PDA has a structural stabilization effect and photothermal conversion characteristics, and the antibacterial effect is enhanced by generating heat through near-infrared light irradiation, and the antibacterial effect can be better exerted.
[0041] In some embodiments, in step one, AlCl3 and adenine polyA 20 are mixed and heated to obtain Al-polyA 20 nanofibers, including: dissolving AlCl3 and polyA 20 in solvents respectively to obtain an AlCl3 solution and a polyA 20 solution; mixing and heating the AlCl3 solution and the polyA 20 solution to obtain Al-polyA 20 nanofibers.
[0042] In some embodiments, in step one, the solvent is a Tris buffer solution containing 10 mM magnesium ions (Mg 2+ ), with a pH of 7-9; the reaction concentration of the AlCl3 solution is 10-100 mM; the reaction concentration of the polyA 20 solution is 10-80 μM.
[0043] Exemplarily, for the Tris buffer solution containing 10 mM magnesium ions, the pH can be 7.3, 7.5, 8.`, 8.4, or 8.6.
[0044] In some embodiments, in step one, the heating temperature is 90 - 100 °C and the reaction time is 1 - 3 h. Preferably, the heating temperature is 95 °C and the reaction time is 2 h.
[0045] In some embodiments, in step one, the AlCl3 solution and the polyA 20 solution are mixed at a molar ratio of 900:1 - 1100:1.
[0046] In some embodiments, in step two, the concentration of the DA solution is 0.5 mg / mL.
[0047] In some embodiments, in step two, the dispersion concentration of the Al-polyA 20 nano-fibers is 10 - 100 nM aluminum ion equivalent concentration.
[0048] In some embodiments, in step three, the Al-polyA 20 nano-fibers are dispersed into the DA solution, and the time for stirring in the dark is 2 - 4 h; centrifugation is carried out using ultrapure water, the centrifugation speed is 10000 - 14000 rpm, the centrifugation time is 10 - 15 min, and the number of washing times is 3 - 4 times.
[0049] Preferably, the Al-polyA 20 composite nano-fibers are dispersed into the DA solution, mixed, stirred in the dark for 3 h, centrifuged using ultrapure water, the centrifugation speed is 12000 rpm, the centrifugation time is 12 min, and centrifugally washed with water 3 - 4 times until the supernatant is clear, and then freeze-dried to obtain the Al-polyA 20 @PDA nano-fibers.
[0050] In addition, the embodiments of the present application also provide an aluminum ion composite nano-fiber with antibacterial properties, which is prepared by using the preparation method of the aluminum ion composite nano-fiber with antibacterial properties described in the above embodiments.
[0051] In some embodiments, the aluminum ion composite nano-fiber has the following characteristics:
[0052] The surface charge is -22.8 mV; the conditions for photothermal conversion are: irradiated with 808 nm near-infrared light with a power of 0.5 - 0.9 W / cm 2 for 1 - 5 min, and the temperature rise is 0 - 35 °C; preferably 0.6 W / cm 2 , irradiated for 3 min.
[0053] The antibacterial conditions are: the sample concentration is 0 - 20 mM, irradiated with 808 nm near-infrared light with a power of 0.6 W / cm 2 for 2 - 4 min, and the minimum inhibitory concentration in Staphylococcus aureus is 8 mM. The present application selects 8 mM as the sample concentration, 0.6 W / cm2 Irradiate with near-infrared light for 3 min.
[0054] In addition, an embodiment of the present application also provides an application of the aluminum ion composite nanofiber with antibacterial properties described in the above embodiment in an antibacterial drug preparation. Specifically, the nanofiber antibacterial agent prepared in the present application can inhibit the growth of bacteria, thereby promoting wound repair treatment.
[0055] The following is a detailed introduction to the preparation method of the aluminum ion composite nanofiber with antibacterial properties provided by the present application through specific examples.
[0056] Example 1: Preparation of Al-polyA 20 Preparation
[0057] Measure 0.4 mL of 1 M Tris-HCl solution and dilute it to 19.6 mL of ultrapure water to prepare a 20 mM Tris buffer solution. Then weigh 0.04066 g of MgCl2·6H2O and dissolve it therein, and adjust the pH to 7.4 with dilute HCl to prepare a 20 mM Tris buffer solution containing 10 mM magnesium ions.
[0058] Weigh a certain amount of AlCl3 (Mw: 133.34 g / mol) and polyA 20 (Mw: 6281.21 g / mol, sequence: AAAAAAAAAAAAAAAAAAAA), dissolve them in the above Tris buffer solution containing magnesium ions, and respectively prepare a 200 mM AlCl3 solution and a 200 μM polyA 20 solution. Then add 5.5 μL of Tris buffer containing magnesium ions, 22.5 μL of AlCl3 solution, and 72 μL of polyA 20 solution to a 200 μL reaction tube, shake and mix well, place it in a constant temperature metal bath, react at 95 °C for 2 h, and cool to room temperature after the reaction to obtain an Al-polyA 20 nanofiber solution.
[0059] Example 2: Preparation of Al-polyA 20 @PDA
[0060] Weigh 60.5 mg of Tris-HCl powder, dissolve it in 50 mL of deionized water, and adjust the pH to 8.6 with dilute HCl to prepare a 10 mM Tris buffer solution with pH = 8.5.
[0061] Take 100 μL of the prepared Al-polyA 20The nanofiber solution was dispersed into 10 mL of the prepared Tris buffer solution (10 mM, pH = 8.6). Then, 5 mg of dopamine (DA) monomer was weighed and dissolved in the mixture. After stirring in the dark for 3 h, it was centrifuged and washed 3 times with deionized water at 14000 rpm for 12 min to obtain Al-polyA with a concentration of 20 mM. 20 @PDA nanofiber solution. The TEM image was taken as Figure 1 shown. It can be seen that the fiber structure of Al-polyA 20 and Al-polyA 20 coated with PDA presents a core-shell structure. The Zeta potential diagram of Al-polyA20@PDA nanofibers is as Figure 2 shown. For Al-polyA 20 successfully coated with PDA, the potential changes from positive charge to negative charge.
[0062] Example 3. Photothermal performance test experiment of Al-polyA 20 @PDA
[0063] Take 150 μL of Al-polyA 20 @PDA nanofiber solution with an aluminum ion equivalent concentration of 8 mM in a 1.5 mL centrifuge tube. Place it under near-infrared lasers with different powers (808 nm, 0.5, 0.6, 0.7, 0.8, 0.9 W / cm 2 ) for irradiation for 5 min, observe the temperature increase on the thermal imager and record it. Then, take 150 μL of Al-polyA 20 @PDA nanofiber solutions with aluminum ion equivalent concentrations of 0, 1, 4, 8, 12, and 16 mM respectively in a 1.5 mL centrifuge tube. Use deionized water as the blank control, and irradiate it with a near-infrared laser (808 nm, 0.6 W / cm 2 ) for 5 min, observe the temperature increase on the thermal imager and record it. Plot the temperature change curve to evaluate the photothermal performance of the sample. [[ID=…]]
[0064] Absorb 150 μL of the aqueous solution of Al-polyA 20 @PDA nanofibers (8 mM aluminum ion equivalent) into a 1.5 mL centrifuge tube. Irradiate it with a near-infrared laser (808 nm, 0.6 W / cm 2 ) for 5 min, and record the temperature increase of Al-polyA 20 @PDA every 1 min. Then remove the 808 nm near-infrared laser, and let the sample cool naturally to near the initial temperature. During this period, record the temperature decrease of Cu-CpG@PDA@HA every 1 min. Repeat the same operation 5 times, and plot the temperature change of Al-polyA 20@PDA temperature change curve. The photothermal properties of Al-polyA20@PDA nanofibers are as Figures 3A to 3C shown, where Figure 3A is the schematic diagram of photothermal properties at different powers, Figure 3B is the schematic diagram of photothermal properties at different concentrations, Figure 3C is the schematic diagram of photothermal stability.
[0065] Example 4. Antibacterial plate experiment
[0066] Staphylococcus aureus (S. aureus) was cultured overnight in tryptic soy broth (TSB) medium on a shaker (37 °C, 250 rpm), and the bacteria in the logarithmic growth phase were obtained by centrifugation. The bacterial concentration was monitored by measuring the OD 600nm value. When the OD600 value of the bacteria was diluted to 0.1, the concentration of Staphylococcus aureus was 2×108 CFU / mL. Subsequently, 100 μL of the bacterial suspension of S. aureus with a concentration of 2×108 CFU / mL was mixed evenly with 100 μL of Al-polyA 20 @PDA with different concentrations (0, 1, 4, 8, 12, 16 mM equivalent aluminum ions) in a 1.5 mL centrifuge tube. Sterile PBS was used as the control group (the phototherapy group was irradiated with 0.6 W of 808 nm near-infrared light for 3 min), and then placed in a shaker at 37 °C and 250 rpm for co-incubation for 1 h. After the incubation, the mixed liquid of each concentration of Al-polyA 20 @PDA and bacteria was diluted 20,000 times, 100 μL was taken and evenly coated on the corresponding bacterial culture medium plate. Each group had 3 parallel samples. After culturing in a biochemical incubator at 37 °C for 12 h, colony counting was performed. The colony images of Staphylococcus aureus incubated with different concentrations of Al-polyA 20 @PDA nanofiber materials with and without near-infrared irradiation are as Figure 4A shown, and the quantitative data are as Figure 4B shown.
[0067] Escherichia coli (E. coli) was cultured overnight in LB broth medium on a shaker (37 °C, 250 rpm), and the bacteria in the logarithmic growth phase were obtained by centrifugation. The bacterial concentration was monitored by measuring the OD 600nm value. When the OD600 value of the bacteria was diluted to 0.1, the concentration of Escherichia coli was 2×10 8 CFU / mL. Subsequently, 100 μL of the bacterial suspension of E. coli with a concentration of 2×10 8 CFU / mL was mixed evenly with 100 μL of Al-polyA 20The @PDA was placed in a 1.5 mL centrifuge tube and mixed evenly. Sterile PBS was used as the control group (the phototherapy group was irradiated with 0.6 W of 808 nm near-infrared light for 3 min), and then it was placed in a shaker at 37 °C at 250 rpm and co-incubated for 1 h. After the incubation, Al-polyA at each concentration was taken 20 The mixed liquid of @PDA and bacteria was diluted 20,000 times, and 100 μL was taken and evenly spread on the corresponding bacterial culture medium plate. Each group was set with 3 parallel samples. After culturing in a biochemical incubator at 37 °C for 12 h, colony counting was carried out. Different concentrations of Al-polyA 20 The colony images of Escherichia coli incubated with the @PDA nanofiber material with and without near-infrared irradiation are as Figure 5A shown, and the quantitative data are as Figure 5B shown.
[0068] Example 5. Cytotoxicity experiment
[0069] The cryopreserved mouse fibroblasts (L929) were taken out, placed in sterile water at 37 °C and shaken back and forth to thaw. Then, 5 mL of DMEM serum medium was aspirated into a centrifuge tube, the thawed cells were added, and the supernatant was removed by centrifugation (1000 rpm, 5 min). The cell pellet at the bottom was resuspended, and the cell suspension was placed in a cell culture dish containing 6 - 7 mL of DMEM serum medium for cultivation. When the cell viability was the best after passage, the cytotoxicity verification experiment of Al-polyA 20 @PDA nanofibers was carried out. The counted cells were inoculated into a 96-well plate (1×104 cells / well), and then placed in a cell culture incubator at 37 °C (5% CO2) for incubation for 12 h. Different concentrations of Al-polyA 20 @PDA nanofiber solutions (0, 1, 4, 8, 12, 16 mM aluminum ion equivalent) were prepared with DMEM as the solvent. The solution in the well plate was changed to 100 μL of different concentrations of Al-polyA20@PDA per well, and the DMEM medium alone was used as the control. After culturing for 24 h, the upper sample solution in the 96-well plate was aspirated, and 120 μL of DMEM-MTT solution (DMEM:MTT = 1:6) was added. Then, the 96-well plate was placed in the cell culture incubator in the dark for 4 h. After the staining was completed, the upper MTT solution was removed, 150 μL of DMSO solution was added to the 96-well plate, and then shaken until the formazan was completely and evenly dissolved in DMSO. The absorbance value at 490 nm was measured with an enzyme-linked immunosorbent assay instrument to determine the cell survival rate. The cytotoxicity of the Al-polyA20@PDA nanofiber material to L929 cells is as Figure 6 shown.
[0070] With the above technical solutions, the present application provides an aluminum ion composite nanofiber with antibacterial properties, its preparation method and application. The preparation method includes the following steps: First, AlCl3 and polyA20 Heat after mixing to obtain Al-polyA 20 nanofibers; then, dissolve DA in Tris buffer solution to obtain a DA solution; finally, disperse the Al-polyA 20 nanofibers into the DA solution, and after mixing, successively carry out light-shielding stirring, centrifugation, and washing to obtain Al-polyA 20 @PDA nanofibers.
[0071] This application prepares metal-nucleic acid composite nanofibers through the co-assembly of aluminum ions and nucleic acid segments, and then prepares composite nanofibers with a PDA coating through in-situ polymerization to prepare a wound-targeted composite nanofiber antibacterial agent. The prepared aluminum ion composite nanofibers loaded with antibacterial properties have good biocompatibility, photothermal conversion effect, and antibacterial characteristics, and can be used to efficiently inhibit bacterial growth and promote the effect of wound repair treatment.
[0072] Those of ordinary skill in the art can understand that the above embodiments are specific examples for implementing this application, and in practical applications, various changes can be made in form and details without departing from the spirit and scope of this application. Any person skilled in the art can make their respective changes and modifications without departing from the spirit and scope of this application. Therefore, the protection scope of this application should be subject to the scope defined by the claims.
Claims
1. A preparation method of aluminum ion composite nanofibers with antibacterial properties, characterized in that, It includes the following steps: Mix AlCl3 and polyA 20 Heat the mixture to obtain Al-polyA 20 nanofibers; Dissolve DA in Tris buffer solution to obtain DA solution; Disperse Al-polyA 20 nanofibers into the DA solution. After mixing, stir in the dark, centrifuge, and wash successively to obtain Al-polyA 20 @PDA nanofibers.
2. The preparation method of the aluminum ion composite nanofiber with antibacterial properties according to claim 1, characterized in that, Mix AlCl3 and adenine polyA 20 Heat the mixture to obtain Al-polyA 20 nanofibers, including: Dissolve AlCl3 and polyA 20 in solvents respectively to obtain an AlCl3 solution and a polyA 20 solution; Mix the AlCl3 solution and the polyA 20 solution, heat them, and then prepare Al-polyA20 nanofibers.
3. The preparation method of the aluminum ion composite nanofibers with antibacterial properties according to claim 2, characterized in that, The solvent is Tris buffer solution containing 10 mM magnesium ions, with a pH of 7 - 9; The reaction concentration of the AlCl3 solution is 10 - 100 mM; The polyA 20 The reaction concentration of the solution is 10 to 80 μM.
4. The preparation method of the aluminum ion composite nanofibers with antibacterial properties according to claim 2, characterized in that, The heating temperature is 90 - 100 °C, and the reaction time is 1 - 3 h.
5. The preparation method of the aluminum ion composite nanofiber with antibacterial properties according to claim 2, characterized in that, Mix the AlCl3 solution and the polyA 20 solutions in a molar ratio of 900:1 to 1100:
1.
6. The preparation method of the aluminum ion composite nanofiber with antibacterial property according to claim 1, characterized in that, The concentration of the DA solution is 0.5 mg / mL.
7. The preparation method of the aluminum ion composite nanofibers with antibacterial properties according to claim 1, characterized in that, The Al-polyA 20 The dispersion concentration of the nanofibers is 10 to 100 nM in terms of aluminum ion equivalent concentration; Disperse Al-polyA 20 in the DA solution, and stir in the dark for 2 to 4 hours; Centrifuge with ultrapure water at a rotational speed of 10000 - 14000 rpm for 10 - 15 min, and wash 3 - 4 times.
8. An aluminum ion composite nanofiber with antibacterial properties, characterized in that, It is prepared by using the preparation method of aluminum ion composite nanofibers with antibacterial properties according to any one of claims 1 to 7.
9. The aluminum ion composite nanofiber with antibacterial properties according to claim 8, characterized in that, The aluminum ion composite nanofibers have the following characteristics: The surface charge is -22.8 mV; The conditions for photothermal conversion are as follows: irradiated with 808 nm near-infrared light with a power of 0.5 - 0.9 W / cm 2 for 1 - 5 min, and the temperature is raised to 0 - 35 °C; The antibacterial conditions are as follows: the sample concentration is 0 - 20 mM, the power is 0.6 W / cm 2 irradiated with 808 nm near-infrared light of for 2 - 4 min, and the minimum inhibitory concentration in Staphylococcus aureus is 8 mM.
10. Application of the aluminum ion composite nanofibers with antibacterial properties according to claim 9 in antibacterial drug preparations.