Rotary gram staining device

Abstract

The invention belongs to the technical field of microbiological inspection instruments, and particularly relates to a rotary gram staining device which comprises a supporting assembly, the slide placing part comprises a slide mounting bin and a slide mounting rack; the slide mounting rack is arranged in the slide mounting bin; the slide mounting bin is hinged to the supporting assembly; one end of the dyeing system is inserted into the top of the supporting assembly; the dyeing system comprises a rotating test bed and a rotating unit; a drying part, a primary dyeing part, a mordant dyeing part, a decoloring part, a redyeing part and a washing part are arranged on the side wall of the rotary test bed; the rotating unit drives the rotating test bed to rotate under the adjustment of the control device, so that one of the drying part, the primary dyeing part, the mordant dyeing part, the decolorizing part, the redyeing part and the washing part is aligned with the slide placing part.

Description

Technical Field

[0001] The invention belongs to the technical field of microbiological testing instruments, and in particular relates to a rotary Gram staining device. Background Art

[0002] Gram staining is a widely used differential staining method in bacteriology, developed by Danish physician Gram in 1884. Unstained bacteria are extremely difficult to observe under a microscope due to the minimal difference in refractive index between them and their surroundings. Staining creates a sharp contrast between the bacteria and their surroundings, allowing for clear observation of their morphology, arrangement, and certain structural features, enabling classification and identification. Gram staining is a counterstaining method.

[0003] Gram staining generally includes four steps: primary staining, mordanting, decolorization, and counterstaining. The specific operation method is:

[0004] 1) Smear fixation;

[0005] 2) Stain with ammonium oxalate crystal violet for 1-2 minutes;

[0006] 3) Washing with water;

[0007] 4) Add iodine solution and cover the surface with dye for 1-2 minutes;

[0008] 5) Washing;

[0009] 6) Add a few drops of 95% alcohol and rinse with water after 20-30 seconds;

[0010] 7) Stain with dilute safranin solution for 1 minute, then rinse with tap water. Dry and examine under a microscope.

[0011] After primary staining with crystal violet and mordanting with iodine solution, a water-insoluble complex of crystal violet and iodine is formed in the cell wall. Gram-positive bacteria have thicker cell walls, more layers of peptidoglycan network and dense cross-linking. Therefore, when they are decolorized with ethanol or acetone, the mesh holes will shrink due to water loss. In addition, they do not contain lipids, so there will be no gaps when treated with ethanol. Therefore, the crystal violet and iodine complex can be firmly retained in the wall, making it still purple. Gram-negative bacteria have thin cell walls, high lipid content in the outer membrane layer, thin peptidoglycan layer and poor cross-linking. After encountering the decolorizer, the outer membrane mainly composed of lipids dissolves rapidly, and the thin and loose peptidoglycan network cannot prevent the dissolution of the crystal violet and iodine complex. Therefore, they remain colorless after decolorization with ethanol, and then re-stain with red dyes such as safranin, which makes the Gram-negative bacteria red.

[0012] The traditional staining method is performed manually by technicians. The operation process is complicated and the staining results vary greatly, which reduces the accuracy of the experimental results. Summary of the Invention

[0013] In view of the above problems, the present application discloses a rotary Gram staining device.

[0014] A rotary Gram staining device, comprising:

[0015] A support assembly;

[0016] A glass slide placement part, including a glass slide installation bin and a glass slide installation rack; the glass slide installation rack is arranged inside the glass slide installation bin; the glass slide installation bin is hinged to the support assembly;

[0017] A staining system, one end of which is inserted into the top of the support assembly; the staining system includes a rotating test bench and a rotating unit; a drying part, a primary staining part, a mordanting part, a decolorizing part, a counterstaining part and a rinsing part are arranged on the side wall of the rotating test bench; the rotating unit drives the rotating test bench to rotate under the adjustment of a control device, so that one of the drying part, the primary staining part, the mordanting part, the decolorizing part, the counterstaining part and the rinsing part is aligned with the glass slide placement part.

[0018] Furthermore, an inclination assembly is arranged inside the glass slide installation bin; a fixed arm is arranged inside the glass slide installation bin; one end of the glass slide installation rack is hinged to the fixed arm, and the other end is hinged to an instrument assembly.

[0019] Furthermore, the staining system further includes a liquid storage assembly; the liquid storage assembly is arranged on the top of the rotating test bench; the liquid storage assembly includes a rinsing bin, a primary staining bin, a mordanting bin, a decolorizing bin and a counterstaining bin; the rinsing bin is connected to the rinsing part; the primary staining bin is connected to the primary staining part; the mordanting bin is connected to the mordanting part; the decolorizing bin is connected to the decolorizing part; the counterstaining bin is connected to the counterstaining part.

[0020] Furthermore, the primary staining part includes a primary staining pipeline; the top of the primary staining pipeline is communicated with the primary staining bin.

[0021] Furthermore, the mordanting part includes a mordanting pipeline; the top of the mordanting pipeline is communicated with the mordanting bin.

[0022] Furthermore, the decolorizing part includes a decolorizing pipeline; the top of the decolorizing pipeline is communicated with the decolorizing bin.

[0023] Furthermore, the counterstaining part includes a counterstaining pipeline; the top of the counterstaining pipeline is communicated with the counterstaining bin.

[0024] Furthermore, the rinsing part includes a rinsing pipeline; the top of the rinsing pipeline is communicated with the rinsing bin.

[0025] Furthermore, the drying part includes a drying bracket and a dryer; one side of the drying bracket is fixedly connected to the rotating test bench, and the other side is connected to the dryer.

[0026] Further, a waste liquid collection device is arranged inside the support assembly; the waste liquid collection device is located at the bottom of the glass slide placement part; through holes are provided at the bottom of the glass slide placement part.

[0027] Advantages of the present invention: Automatically perform Gram staining operations, saving time for experimental personnel; and each step is carried out according to the set sequence and time, with good uniformity and repeatability of the treatment results, solving the experimental errors caused by manual operations, effectively reducing the difference in staining results, and improving the accuracy of experimental results. Traditional Gram staining is generally carried out by the pool due to the reagent pollution. Although microorganisms need to be inactivated, the uncertainty of manual operations may cause unnecessary personnel infections or laboratory pollution due to contamination. The automated Gram staining instrument enables the staining operation to be carried out in a closed environment, thus eliminating cross-contamination in the laboratory and achieving better biosafety. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic structural diagram of an automated Gram staining instrument for implementing the present invention;

[0029] Figure 2 It is a schematic structural diagram of an automated Gram staining instrument for implementing the present invention when it is in an open state;

[0030] Figure 3 It is a cross-sectional view of an automated Gram staining instrument for implementing the present invention;

[0031] Figure 4 It is a schematic structural diagram of a staining system for implementing the present invention;

[0032] Figure 5 It is a top view of the internal pipeline of a staining system for implementing the present invention

[0033] Figure 6 It is a schematic diagram of the internal pipeline of a staining system for implementing the present invention;

[0034] Figure 7 It is a schematic diagram of the connection of the internal pipeline of a staining system for implementing the present invention;

[0035] Figure 8 It is a schematic structural diagram of the glass slide placement part for implementing the present invention when it is in a horizontal state;

[0036] Figure 9 It is a real - shot picture of the glass slide placement part for implementing the present invention when it is in a horizontal state;

[0037] Figure 10 It is a schematic structural diagram of the glass slide placement part for implementing the present invention when it is in an inclined state;

[0038] Figure 11 It is a cross-sectional view when the glass slide placement part for implementing the present invention is in an inclined state;

[0039] In the figure, 1 is the glass slide placement part; 2 is the support assembly; 3 is the staining system; 4 is the waste liquid collection device; 5 is the control device; 11 is the glass slide installation bin; 12 is the glass slide installation rack; 13 is the telescopic motor; 21 is the bottom housing; 22 is the top housing; 31 is the liquid storage assembly; 32 is the dust cover; 33 is the drying part; 34 is the primary staining part; 35 is the mordanting part; 36 is the decolorizing part; 37 is the counterstaining part; 38 is the rinsing part; 51 is the controller; 52 is the control module; 111 is the fixed arm; 112 is the partition; 113 is the lifting arm; 114 is the lifting bracket; 121 is the limiting structure; 311 is the rinsing chamber; 312 is the primary staining chamber; 313 is the mordanting chamber; 314 is the decolorizing chamber; 315 is the counterstaining chamber; 341 is the primary staining pipeline; 342 is the primary staining valve; 351 is the mordanting pipeline; 352 is the mordanting valve; 361 is the decolorizing pipeline; 362 is the decolorizing valve; 371 is the counterstaining pipeline; 372 is the counterstaining valve; 381 is the rinsing pipeline; 382 is the rinsing valve. Specific embodiments

[0040] To make the objectives, technical solutions and advantages of this application clearer, the technical solutions of this application will be clearly and completely described below in conjunction with specific embodiments of this application and the corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of this application, rather than all the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application.

[0041] In this article, "schematic" means "serving as an example, instance or illustration", and any illustration or implementation mode described as "schematic" in this article should not be interpreted as a more preferred or more advantageous technical solution.

[0042] To make the drawings concise, only the parts related to this application are schematically shown in each drawing, and they do not represent the actual structure of the product. In addition, to make the drawings concise and easy to understand, in some drawings, components with the same structure or function are only schematically shown for one of them, or only one of them is marked.

[0043] In this text, it should be understood that the orientation or positional relationships indicated by terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. are based on the orientation or positional relationships shown in the drawings. These are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation on the present invention.

[0044] In this text, unless otherwise clearly specified and defined, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance; unless otherwise specified or stated, the term "plural" means two or more; the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, an integral connection, or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0045] As Figures 1-11 shown, a rotary Gram staining device includes: a support assembly 2, a slide placement part 1, a staining system 3, and a control device 5. The slide placement part 1 includes a slide mounting bin 11 and a slide mounting rack 12; the slide mounting rack 12 is arranged inside the slide mounting bin 11; the slide mounting bin 11 is hinged to the support assembly 2; one end of the staining system 3 is inserted into the top of the support assembly 2; the staining system 3 includes a rotating test bench and a rotating unit; a drying part 33, a primary staining part 34, a mordanting part 35, a decolorizing part 36, a counterstaining part 37, and a rinsing part 38 are arranged on the side wall of the rotating test bench; the rotating unit drives the rotating test bench to rotate under the adjustment of the control device 5, so that one of the drying part 33, the primary staining part 34, the mordanting part 35, the decolorizing part 36, the counterstaining part 37, and the rinsing part 38 is aligned with the slide placement part 1. The control device 5 is arranged inside the support assembly 2; the control device 5 is connected to the tilting assembly and the rotating unit through wires, so that the control device 5 controls one or several of the tilting assembly, the rotating unit, the drying part 33, the primary staining part 34, the mordanting part 35, and the decolorizing part 36 to complete their respective corresponding operations.

[0046] Furthermore, an alarm is provided on the support assembly 2. The alarm is connected to the control device 5 through a wire.

[0047] Automatically perform Gram staining operations, saving time for experimenters; and each step is carried out in accordance with the set order and time, with good uniformity and repeatability of the processing results, solving the experimental errors caused by manual operations, effectively reducing the differences in staining results, and improving the accuracy of experimental results. Traditional Gram staining is generally carried out by the pool due to reagent contamination. Although microorganisms need to be inactivated, the uncertainty of manual operations may cause unnecessary personnel infections or laboratory contamination. The automated Gram staining instrument enables the staining operation to be carried out in a closed environment, thus eliminating cross-contamination in the laboratory and achieving better biosafety.

[0048] Specifically, the rotating unit is a rotating motor (not marked in the figure), and the rotating motor rotates under the control of the control device 5.

[0049] Specifically, the number of the slide mounting racks 12 is A, and A≥1. As Figures 2-3 shown, A = 3.

[0050] As Figure 2 shown, the slide mounting rack 12 is provided with a groove (not marked in the figure). A limiting structure 121 is rotatably connected to the side wall of the slide mounting rack 12. After placing the slide with the sample into the inside of the groove, the limiting structure 121 can be rotated to press the slide against the inside of the groove, preventing the slide from being displaced during the staining process.

[0051] As Figures 1-3 shown, in order to facilitate the collection of used primary stain, mordant, decolorizer, counterstain, and rinsing solution, a waste liquid collection device 4 is arranged inside the support assembly 2; the waste liquid collection device 4 is located at the bottom of the slide placement part 1. A collection hole (not marked in the figure) is arranged at the bottom of the slide mounting chamber 11, and the used primary stain, mordant, decolorizer, counterstain, and rinsing solution flow into the waste liquid collection device 4 through the collection hole, facilitating the centralized treatment of waste liquid.

[0052] Specifically, the waste liquid collection device 4 is slidably connected to the support assembly 2. The waste liquid collection device 4 can be taken out from the side wall of the support assembly 2, which is convenient and fast.

[0053] As Figures 2-3 shown, the support assembly 2 includes a bottom housing 21 and a top housing 22; the bottom housing 21 is arranged at the bottom of the top housing 22; the slide placement part 1 is hinged to the top housing 22. The waste liquid collection device 4 is slidably connected to the bottom housing 21. So that the waste liquid generated during the staining process enters the waste liquid collection device 4 under the action of gravity, facilitating the centralized treatment of waste liquid.

[0054] The control device 5 includes a controller 51 and a control module 52, and the controller 51 and the control module 52 are connected by wires; during use, the operating time of each step is set through the controller 51, and the control module 52 drives one or several of the tilting assembly, the rotating unit, the drying unit 33, the primary dyeing unit 34, the mordanting unit 35, the decolorizing unit 36, and the re-dyeing unit 37 to complete their respective corresponding operations, so as to achieve Gram staining of the glass slides.

[0055] As Figure 2 shown, the drying unit 33 includes a drying bracket (not marked in the figure) and a dryer (not marked in the figure); one side of the drying bracket is fixedly connected to the rotating test bench, and the other side is connected to the dryer. When the drying unit 33 is aligned with the glass slide mounting rack 12, the dryer starts to work to dry the glass slides.

[0056] As Figures 5-7 shown, the staining system 3 further includes a liquid storage assembly 31 and a dust-proof cover 32, and the dust-proof cover 32 is adapted to the liquid storage assembly 31 to prevent external dust from entering the inside of the liquid storage assembly 31 during the operation of the staining system 3, thereby contaminating the glass slides.

[0057] The liquid storage assembly 31 is arranged on the top of the rotating test bench; the liquid storage assembly 31 includes a rinsing chamber 311, a primary dyeing chamber 312, a mordanting chamber 313, a decolorizing chamber 314, and a re-dyeing chamber 315; the rinsing chamber 311 is connected to the rinsing unit 38; the primary dyeing chamber 312 is connected to the primary dyeing unit 34; the mordanting chamber 313 is connected to the mordanting unit 35; the decolorizing chamber 314 is connected to the decolorizing unit 36; the re-dyeing chamber 315 is connected to the re-dyeing unit 37.

[0058] During use, the rinsing liquid is stored inside the rinsing chamber 311, the primary dyeing liquid is stored inside the primary dyeing chamber 312, the mordanting liquid is stored inside the mordanting chamber 313, the decolorizing liquid is stored inside the decolorizing chamber 314, and the re-dyeing liquid is stored inside the re-dyeing chamber 315. During the automatic staining process, the rotating unit drives the rotating test bench to rotate, so that one of the primary dyeing unit 34, the mordanting unit 35, the decolorizing unit 36, the re-dyeing unit 37, and the rinsing unit 38 is aligned with the glass slide mounting rack 12, and a primary dyeing liquid, a mordanting liquid, a decolorizing liquid, a re-dyeing liquid, or a rinsing liquid is applied to the glass slides.

[0059] Preferably, a liquid level sensor (not shown in the figure) is provided inside each of the rinsing chamber 311, the primary dyeing chamber 312, the mordanting chamber 313, the decolorizing chamber 314, and the re-dyeing chamber 315. When the liquid level in one or more of the rinsing chamber 311, the primary dyeing chamber 312, the mordanting chamber 313, the decolorizing chamber 314, and the re-dyeing chamber 315 is too low, the liquid level sensor sends a message to the control device 5, and the control device 5 turns on the alarm to remind the experimenter to add the appropriate liquid.

[0060] As Figures 5-7As shown, the primary dyeing section 34 includes a primary dyeing pipeline 341; the top of the primary dyeing pipeline 341 communicates with the primary dyeing bin 312. The primary dyeing liquid inside the primary dyeing bin 312 flows out through the primary dyeing pipeline 341 under the drive of gravity.

[0061] A primary dyeing valve 342 is provided at the top of the primary dyeing pipeline 341. The primary dyeing valve 342 is connected to the control device 5 through a wire. In the normal state, the primary dyeing valve 342 is in a closed state to block the outflow of the primary dyeing liquid. When the primary dyeing section 34 is aligned with the slide mounting rack 12, the primary dyeing valve 342 opens, so that the primary dyeing liquid inside the primary dyeing bin 312 flows out through the primary dyeing pipeline 341 and is applied to the slide to complete the primary dyeing operation. By controlling the opening time of the primary dyeing valve 342, the control device 5 can control the discharge amount of the primary dyeing liquid.

[0062] Preferably, a primary dyeing branch is provided on the side of the primary dyeing pipeline 341 away from the rotation test bench; the primary dyeing branch extends in the direction away from the rotation test bench. The number of primary dyeing branches is equal to the number of slide mounting racks 12, so that the primary dyeing section 34 can apply the primary dyeing liquid to each slide on the slide mounting rack 12 simultaneously.

[0063] As Figures 5-7 shown, the mordanting section 35 includes a mordanting pipeline 351; the top of the mordanting pipeline 351 communicates with the mordanting bin 313. The mordanting liquid inside the mordanting bin 313 flows out through the mordanting pipeline 351 under the drive of gravity.

[0064] A mordanting valve 352 is provided at the top of the mordanting pipeline 351. The mordanting valve 352 is connected to the control device 5 through a wire. In the normal state, the mordanting valve 352 is in a closed state to block the outflow of the mordanting liquid. When the mordanting section 35 is aligned with the slide mounting rack 12, the mordanting valve 352 opens, so that the mordanting liquid inside the mordanting bin 313 flows out through the mordanting pipeline 351 and is applied to the slide to complete the mordanting operation. By controlling the opening time of the mordanting valve 352, the control device 5 can control the discharge amount of the mordanting liquid.

[0065] Preferably, a mordanting branch is provided on the side of the mordanting pipeline 351 away from the rotation test bench; the mordanting branch extends in the direction away from the rotation test bench. The number of mordanting branches is equal to the number of slide mounting racks 12, so that the mordanting section 35 can apply the mordanting liquid to each slide on the slide mounting rack 12 simultaneously.

[0066] As Figures 5-7 shown, the decolorizing section 36 includes a decolorizing pipeline 361; the top of the decolorizing pipeline 361 communicates with the decolorizing bin 314. The decolorizing liquid inside the decolorizing bin 314 flows out through the decolorizing pipeline 361 under the drive of gravity.

[0067] A decolorization valve 362 is provided at the top of the decolorization pipeline 361. The decolorization valve 362 is connected to the control device 5 through a wire. In the normal state, the decolorization valve 362 is in a closed state to block the outflow of the decolorization liquid. When the decolorization part 36 is aligned with the slide mounting rack 12, the decolorization valve 362 opens, so that the decolorization liquid inside the decolorization chamber 314 flows out through the decolorization pipeline 361 and is applied to the slide to complete the decolorization operation. By controlling the opening time of the decolorization valve 362, the control device 5 can control the discharge amount of the decolorization liquid.

[0068] Preferably, a decolorization branch is provided on the side of the decolorization pipeline 361 away from the rotation test bench; the decolorization branch extends in the direction away from the rotation test bench. The number of decolorization branches is equal to the number of slide mounting racks 12, so that the decolorization part 36 can apply the decolorization liquid to each slide on the slide mounting rack 12 simultaneously.

[0069] As Figures 5-7 shown, the counterstaining part 37 includes a counterstaining pipeline 371; the top of the counterstaining pipeline 371 is communicated with the counterstaining chamber 315. The counterstaining liquid inside the counterstaining chamber 315 flows out through the counterstaining pipeline 371 under the drive of gravity.

[0070] A counterstaining valve 372 is provided at the top of the counterstaining pipeline 371. The counterstaining valve 372 is connected to the control device 5 through a wire. In the normal state, the counterstaining valve 372 is in a closed state to block the outflow of the counterstaining liquid. When the counterstaining part 37 is aligned with the slide mounting rack 12, the counterstaining valve 372 opens, so that the counterstaining liquid inside the counterstaining chamber 315 flows out through the counterstaining pipeline 371 and is applied to the slide to complete the counterstaining operation. By controlling the opening time of the counterstaining valve 372, the control device 5 can control the discharge amount of the counterstaining liquid.

[0071] Preferably, a counterstaining branch is provided on the side of the counterstaining pipeline 371 away from the rotation test bench; the counterstaining branch extends in the direction away from the rotation test bench. The number of counterstaining branches is equal to the number of slide mounting racks 12, so that the counterstaining part 37 can apply the counterstaining liquid to each slide on the slide mounting rack 12 simultaneously.

[0072] As Figures 5-7 shown, the rinsing part 38 includes a rinsing pipeline 381; the top of the rinsing pipeline 381 is communicated with the rinsing chamber 311. The rinsing liquid inside the rinsing chamber 311 flows out through the rinsing pipeline 381 under the drive of gravity.

[0073] A flushing valve 382 is provided at the top of the flushing pipeline 381. The flushing valve 382 is connected to the control device 5 through a wire. In the normal state, the flushing valve 382 is in a closed state to block the outflow of the flushing liquid. When the flushing part 38 is aligned with the slide mounting rack 12, the flushing valve 382 opens, so that the flushing liquid inside the flushing chamber 311 flows out through the flushing pipeline 381 and is applied to the slide to complete the flushing operation. By controlling the opening time of the flushing valve 382, the control device 5 can control the discharge amount of the flushing liquid.

[0074] Preferably, a flushing branch is provided on the side of the flushing pipeline 381 away from the rotation test bench; the flushing branch extends in the direction away from the rotation test bench. The number of flushing branches is equal to the number of slide mounting racks 12, so that the flushing part 38 can apply the flushing liquid to each slide on the slide mounting rack 12 simultaneously.

[0075] As Figures 8-11 shown, an inclination component is provided inside the slide mounting chamber 11; a fixed arm 111 is provided inside the slide mounting chamber 11; one end of the slide mounting rack 12 is hinged to the fixed arm 111, and the other end is hinged to the instrument component. A fixed arm 111 is provided on both sides of each slide mounting rack 12.

[0076] Specifically, the fixed arm 111 is horizontally arranged, and the inclination component drives the slide mounting chamber 11 to rotate, changing the angle between the slide mounting rack 12 and the fixed arm 111, so that the slide mounting rack 12 can be selectively horizontal or inclined.

[0077] The inclination component includes a lifting arm 113 and a driver; one end of the lifting arm 113 is connected to the driver, and the other end is provided with a chute; a slider is provided on the slide mounting rack 12; the slider moves along the chute; the driver drives the lifting arm 113 to make a reciprocating motion along a straight line.

[0078] The number of fixed arms 111 is equal to the number of lifting arms 113, both are B, and B = 2×A.

[0079] Specifically, the extending direction of the chute is perpendicular to the moving direction of the lifting arm 113. When the lifting arm 113 moves under the drive of the driver, the slider slides inside the chute, driving the slide mounting chamber 11 to rotate and changing the angle between the slide mounting rack 12 and the fixed arm 111.

[0080] Specifically, the driver is a telescopic motor 13.

[0081] As Figures 8-11As shown in the figure, the slide mounting bin 11 includes a placement bin and a movement bin; the placement bin and the movement bin are separated by a partition 112. The driver is located inside the movement bin; the lifting arm 113 passes through the partition 112 and is connected to the slide mounting rack 12; the purpose of this design is to prevent the primary stain solution, mordant solution, decolorizing solution, counterstain solution and rinsing solution from splashing onto the driver and prevent the driver from getting water in.

[0082] Preferably, the tilting assembly further includes a lifting bracket 114; the lifting bracket 114 is located inside the movement bin; one end of the lifting bracket 114 is connected to the driver, and the other end is connected to the lifting arm 113. Driven by the control device 5, the telescopic motor 13 drives the lifting bracket 114 to perform a linear reciprocating motion in the vertical direction. When the lifting bracket 114 rises, it drives the lifting arm 113 to rise, driving the slide mounting bin 11 to rotate, increasing the angle between the slide mounting rack 12 and the fixed arm 111, and making the slide mounting rack 12 enter the tilted state. When the lifting bracket 114 descends, it drives the lifting arm 113 to descend, driving the slide mounting bin 11 to rotate, reducing the angle between the slide mounting rack 12 and the fixed arm 111, and making the slide mounting rack 12 gradually enter the horizontal state.

[0083] Operation process of the automatic Gram staining instrument:

[0084] 1. The rotation unit drives the rotation test bench to rotate, aligning the drying part 33 with the slide placement part 1 to dry the slide with the sample.

[0085] 2. The rotation unit drives the rotation test bench to rotate, aligning the primary staining part 34 with the slide placement part 1, and the tilting assembly makes the slide mounting rack 12 in a horizontal state; the primary staining part 34 drops the primary stain solution onto the horizontal slide so that the primary stain solution can immerse the sample.

[0086] 3. After 1 - 2 minutes, the tilting assembly makes the slide mounting rack 12 in a tilted state, the rotation unit drives the rotation test bench to rotate, aligning the rinsing part 38 with the slide placement part 1, and the rinsing part 38 flows out the rinsing solution to rinse the slide for 10 - 15 seconds.

[0087] 4. The rotation unit drives the rotation test bench to rotate, aligning the mordanting part 35 with the slide placement part 1, and the tilting assembly makes the slide mounting rack 12 in a horizontal state; the mordanting part 35 drops the mordant solution onto the horizontal slide so that the mordant solution can immerse the sample.

[0088] 5. After 1 - 2 minutes, the tilting assembly makes the slide mounting rack 12 in a tilted state, the rotation unit drives the rotation test bench to rotate, aligning the rinsing part 38 with the slide placement part 1, and the rinsing part 38 flows out the rinsing solution to rinse the slide for 10 - 15 seconds.

[0089] 6. The rotation unit drives the rotation test bench to rotate, aligning the decolorization unit 36 with the slide placement unit 1, and the tilting assembly keeps the slide mounting frame 12 in a horizontal state; the decolorization unit 36 drips decolorizing solution onto the horizontal slide so that the decolorizing solution can submerge the sample;

[0090] 7. After 20 - 30 s, the tilting assembly makes the slide mounting frame 12 in an inclined state, and the rotation unit drives the rotation test bench to rotate, aligning the rinsing unit 38 with the slide placement unit 1, and the rinsing unit 38 flows out the rinsing solution to rinse the slide for 10 - 15 s;

[0091] 8. The rotation unit drives the rotation test bench to rotate, aligning the counterstaining unit 37 with the slide placement unit 1, and the tilting assembly keeps the slide mounting frame 12 in a horizontal state; the counterstaining unit 37 drips counterstaining solution onto the horizontal slide so that the counterstaining solution can submerge the sample;

[0092] 9. After 1 min, the tilting assembly makes the slide mounting frame 12 in an inclined state, and the rotation unit drives the rotation test bench to rotate, aligning the rinsing unit 38 with the slide placement unit 1, and the rinsing unit 38 flows out the rinsing solution to rinse the slide for 10 - 15 s;

[0093] 10. The rotation unit drives the rotation test bench to rotate, aligning the drying unit 33 with the slide placement unit 1, and drying the stained slide;

[0094] 11. Issue an alarm to remind the experimenter that the slide staining is completed.

[0095] The usage method of this embodiment:

[0096] 1. Open the slide placement unit 1 and place the slide with the sample into the slide mounting frame 12;

[0097] 2. Rotate the slide placement unit 1 so that the slide placement unit 1 closely adheres to the support assembly 2, making the slide close to the staining system 3;

[0098] 3. Connect the power supply, turn on the control device 5, and set the running time for each step;

[0099] 4. Turn on the automatic Gram staining instrument to automatically stain the slides inside it;

[0100] 5. After the staining is completed, rotate the slide placement unit 1 and take out the stained slide.

[0101] The above is only the specific implementation manner of the present application. Under the above teaching of the present application, those skilled in the art can make other improvements or deformations on the basis of the above embodiments. Those skilled in the art should understand that the above specific description is only a better explanation of the purpose of the present application, and the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A rotary Gram staining device, characterized in that, Comprising: A support component; A glass slide placement part, including a glass slide installation bin and a glass slide installation rack; The glass slide installation rack is arranged inside the glass slide installation bin; The glass slide installation bin is hinged to the support component; A staining system, one end of which is inserted into the top of the support component; the staining system includes a rotating test bench and a rotating unit; a drying part, a primary staining part, a mordanting part, a decolorizing part, a counterstaining part and a rinsing part are arranged on the side wall of the rotating test bench; the rotating unit drives the rotating test bench to rotate under the adjustment of a control device, so that one of the drying part, the primary staining part, the mordanting part, the decolorizing part, the counterstaining part and the rinsing part is aligned with the glass slide placement part.

2. The rotary Gram staining device according to claim 1, wherein, An inclined component is arranged inside the glass slide installation bin; a fixed arm is arranged inside the glass slide installation bin; one end of the glass slide installation rack is hinged to the fixed arm, and the other end is hinged to an instrument component; Preferably, the inclined component includes a lifting arm and a driver; one end of the lifting arm is connected to the driver, and the other end is provided with a chute; a slider is arranged on the glass slide installation rack; the slider moves along the chute; the driver drives the lifting arm to make a reciprocating motion along a straight line; Preferably, the glass slide installation bin includes a placement bin and a motion bin; the placement bin and the motion bin are separated by a partition; Preferably, the driver is located inside the motion bin; the lifting arm passes through the partition and is connected to the glass slide installation rack; Preferably, the inclined component further includes a lifting support; the lifting support is located inside the motion bin; one end of the lifting support is connected to the driver, and the other end is connected to the lifting arm; Preferably, the number of the glass slide installation racks is A, A≥1; Preferably, the number of the fixed arms is equal to the number of the lifting arms, both are B, B = 2×A.

3. The rotary Gram staining device according to claim 1, wherein, The staining system further includes a liquid storage component; the liquid storage component is arranged on the top of the rotating test bench; the liquid storage component includes a rinsing bin, a primary staining bin, a mordanting bin, a decolorizing bin and a counterstaining bin; the rinsing bin is connected to the rinsing part; the primary staining bin is connected to the primary staining part; the mordanting bin is connected to the mordanting part; the decolorizing bin is connected to the decolorizing part; the counterstaining bin is connected to the counterstaining part.

4. A rotary Gram staining device according to claim 3, wherein, The primary staining part includes a primary staining pipeline; the top of the primary staining pipeline is communicated with the primary staining bin; Preferably, a primary staining valve is arranged at the top of the primary staining pipeline; Preferably, a primary staining branch is arranged on the side of the primary staining pipeline away from the rotating test bench; the primary staining branch extends in a direction away from the rotating test bench.

5. The rotary Gram staining device according to claim 3, wherein, The mordanting part includes a mordanting pipeline; the top of the mordanting pipeline is communicated with the mordanting bin; Preferably, a mordanting valve is arranged at the top of the mordanting pipeline; Preferably, a mordanting branch is arranged on the side of the mordanting pipeline away from the rotating test bench; the mordanting branch extends in a direction away from the rotating test bench.

6. The rotary Gram staining device according to claim 3, wherein, The decolorizing part includes a decolorizing pipeline; the top of the decolorizing pipeline is communicated with the decolorizing bin; Preferably, a decolorizing valve is arranged at the top of the decolorizing pipeline; Preferably, a decolorizing branch is arranged on the side of the decolorizing pipeline away from the rotating test bench; the decolorizing branch extends in a direction away from the rotating test bench.

7. A rotary Gram staining device according to claim 3, characterized in that, The counterstaining part includes a counterstaining pipeline; the top of the counterstaining pipeline is communicated with the counterstaining bin; Preferably, a counterstaining valve is provided at the top of the counterstaining pipeline; Preferably, a counterstaining branch is provided on the side of the counterstaining pipeline away from the rotation test bench; the counterstaining branch extends in a direction away from the rotation test bench.

8. The rotary Gram staining device according to claim 3, characterized in that, The rinsing part includes a rinsing pipeline; the top of the rinsing pipeline is communicated with the rinsing chamber; Preferably, a rinsing valve is provided at the top of the rinsing pipeline; Preferably, a rinsing branch is provided on the side of the rinsing pipeline away from the rotation test bench; the rinsing branch extends in a direction away from the rotation test bench.

9. A rotary Gram staining device according to claim 1, characterized in that, The drying part includes a drying bracket and a dryer; one side of the drying bracket is fixedly connected to the rotation test bench, and the other side is connected to the dryer.

10. A rotary Gram staining device according to claim 1, characterized in that, A waste liquid collection device is arranged inside the support assembly; the waste liquid collection device is located at the bottom of the glass slide placement part; through holes are provided at the bottom of the glass slide placement part; Preferably, the waste liquid collection device is slidably connected to the support assembly.

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