Functional food capable of solving tear stains of pets and preparation method of functional food

Abstract

The invention relates to the technical field of pet food processing, and discloses a functional food for solving pet tear stains and a preparation method thereof, and the functional food comprises the following raw materials in parts by mass: 40-50 parts of chicken breast, 10-15 parts of fish meat, 20-25 parts of starch, 30-40 parts of nutritional components, 6-8 parts of vegetable oil, 3-5 parts of carrots, and 1-2 parts of table salt. The prepared pet functional food has a meat-like and faint scent taste, is good in palatability, strong in stability, balanced in nutrition, easy to digest and absorb, and capable of clearing heat and reducing internal heat, effectively relieving formation of pet tear stains, and can improve the fur luster of pets after long-term use, so that the fur luster of the pets is bright, smooth and soft.

Description

Technical Field

[0001] The present invention relates to the technical field of pet food processing, in particular to a functional food for solving pet tear stains and a preparation method thereof. Background Art

[0002] The lacrimal gland is an accessory organ of the eye. Its main function is to secrete tears to moisten the eyeball. Under normal circumstances, the lacrimal gland secretes tears to moisten the eyeball and then enters the nasal cavity through the tear duct. It will not flow out from the corners of the eyes. If the lacrimal gland secretes too many tears or cannot be discharged from the nasolacrimal duct, tears will flow out from the corners of the eyes. If they are not cleaned in time, tear marks will form, which are prone to breed bacteria and cause eye infections. Therefore, adding functional foods to the daily diet has anti-inflammatory, antioxidant, heat-clearing, eyesight-improving, anti-inflammatory and antibacterial effects, which can effectively alleviate the formation of tear marks in pets.

[0003] Arginine and plant extracts are added to pet food to increase the density of macular pigment in the pet's retina, protect the macula, have anti-inflammatory effects, inhibit oxidative damage to tissues such as the lens and macula, and can relieve the formation of tear marks in pet dogs. However, their stability is poor, which affects the quality of pet food. They also have poor binding to the intestinal mucosal mucin of pets and a short retention time, resulting in low bioavailability. Summary of the Invention

[0004] The present invention provides a functional food for solving pet tear stains and a preparation method thereof, thereby solving the problems of low bioavailability and poor stability of functional foods.

[0005] The technical solution of the present invention:

[0006] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 40-50 parts of chicken breast, 10-15 parts of fish, 20-25 parts of starch, 30-40 parts of nutritional components, 6-8 parts of vegetable oil, 3-5 parts of carrots, and 1-2 parts of salt;

[0007] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0008] S1. Add chicken breast, fish, starch, nutrients, vegetable oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0009] S2. The mixture is extruded through an extruder, dried at a temperature of 35-45°C for 6-8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food.

[0010] Furthermore, the starch is selected from any one of corn starch, rice starch, potato starch and tapioca starch.

[0011] Furthermore, the vegetable oil is selected from any one of olive oil, coconut oil, soybean oil, rapeseed oil, sunflower oil and linseed oil.

[0012] Furthermore, the extrusion device is a twin-screw extruder with an extrusion temperature of 70-80° C. and a rotation speed of 270-290 r / min.

[0013] Furthermore, the nutritional components are obtained by embedding arginine and plant extracts into carboxymethyl chitosan microspheres, and then reacting with zinc chloride, polyacrylic acid and wheat germ agglutinin.

[0014] Furthermore, the nutritional components are specifically prepared by the following steps:

[0015] A1. Carboxymethyl chitosan and arginine were added to an acetic acid solution and stirred uniformly. The extract was then added and stirred continuously to obtain an aqueous phase. Liquid paraffin and sorbitan monooleate were mixed and stirred uniformly to obtain an oil phase. The aqueous phase was added to the oil phase and emulsified at 35-45°C for 1.5-2.5 hours. Glutaraldehyde was added and the reaction was stirred for 3-5 hours. The mixture was cooled to room temperature, centrifuged, washed, and dried to obtain chitosan microspheres.

[0016] A2. Add polyacrylic acid and wheat germ agglutinin to deionized water, stir well, place in a water bath, and continue stirring to obtain a mixture;

[0017] A3. Add chitosan microspheres and zinc chloride to deionized water, stir evenly, add the mixture, continue stirring, cool to room temperature, filter, wash, and dry to obtain the nutritional components.

[0018] Furthermore, in the above-mentioned A1 reaction process, liquid paraffin is used as the oil phase medium, sorbitan monooleate is used as the surfactant, and a mixture of arginine, plant extracts and carboxymethyl chitosan is used as the aqueous phase. The aqueous phase is added to the oil phase for emulsification to form a stable water-in-oil emulsion, and then formaldehyde solution is added dropwise, and cross-linked and solidified to form chitosan microspheres.

[0019] Furthermore, during the above reaction A2, the carboxyl groups carried by the polyacrylic acid can combine with the hydrophilic groups of the wheat germ agglutinin through hydrogen bonds to form a complex.

[0020] Furthermore, during the above-mentioned reaction A3, the functional groups carried on the surface of the chitosan microspheres can complex with the zinc ions in the zinc chloride, and the oxygen-containing functional groups in the complex can also complex with the zinc ions, so that the complex is coated on the surface of the chitosan microspheres as a nutritional component.

[0021] Furthermore, in step A1, the mass ratio of carboxymethyl chitosan, arginine, acetic acid solution and extract is (5-6):(2-2.2):(80-85):(8-10).

[0022] Furthermore, in step A1, the volume ratio of liquid paraffin and sorbitan monooleate is (90-110):(0.5-1).

[0023] Furthermore, in step A1, the volume ratio of the water phase to the oil phase is (85-95):(55-60).

[0024] Furthermore, in step A1, the extract is prepared by mixing the plant extract and ethanol in a mass ratio of (2.2-2.4):(10-12).

[0025] Furthermore, the plant extract is prepared by mixing marigold extract, wolfberry extract and cassia seed extract in a mass ratio of 1:1:(1.5-2).

[0026] Furthermore, in step A2, the mass ratio of polyacrylic acid, wheat germ agglutinin and deionized water is (2.4-2.6):(2-2.2):(45-55).

[0027] Furthermore, in step A3, the mass ratio of chitosan microspheres, zinc chloride, deionized water and the mixture is (5-5.6):(1-2):(55-65):(28-32).

[0028] The present invention has the following beneficial effects:

[0029] (1) In the technical solution of the present invention, arginine and plant extracts are embedded in carboxymethyl chitosan microspheres. On the one hand, the stability of arginine and plant extracts is improved, and the poor stability of arginine and plant extracts in the environment is avoided, which reduces the quality of functional foods and affects the efficacy of functional foods in alleviating pet tear marks. On the other hand, arginine and plant extracts can also be cross-linked with carboxymethyl chitosan microspheres to improve the mechanical strength and chemical stability of the microspheres and avoid leakage of active components.

[0030] (2) In the technical solution of the present invention, the plant extracts include marigold extract, wolfberry extract, and cassia seed extract. The lutein contained in marigold extract is a natural antioxidant, known as "eye gold", which can increase the density of retinal macular pigment, protect the macula, inhibit oxidative damage to tissues such as the lens and macula, and can inhibit and scavenge the activity of free radicals; the wolfberry polysaccharide contained in wolfberry extract has antioxidant, antibacterial and immunomodulatory effects, can inhibit the occurrence and deterioration of inflammation, and can effectively alleviate the formation of tear marks in pets; Cassia seed extract contains free anthraquinones, which has the effects of clearing heat and improving eyesight, and anti-inflammatory and antibacterial; the mixed ratio of the three plant extracts has a synergistic effect in inhibiting the formation of tear marks in pets.

[0031] (3) In the technical solution of the present invention, arginine can promote the construction of the intestinal mucosal immune barrier of pets, maintain the balance of normal intestinal flora and probiotics, reduce food allergy intake, reduce the irritation of eye secretions, and synergize with plant extracts to effectively alleviate the formation of tear marks in pets.

[0032] (4) In the technical solution of the present invention, polyacrylic acid and wheat germ agglutinin are deposited on the surface of carboxymethyl chitosan microspheres through zinc ions as nutritional components. On the one hand, polyacrylic acid can enhance the binding of nutritional components to intestinal mucosal mucin, thereby prolonging the retention time of nutritional components in the intestine, ensuring the full absorption of functional foods by pets, improving bioavailability, and effectively alleviating the formation of tear marks in pets. On the other hand, wheat germ agglutinin is a type of protein or glycoprotein that is widely present in nature. It can recognize different sugar groups of pet cell membrane glycolipids or glycoprotein sugar chains, cooperate with polyacrylic acid, enhance the binding ability of nutritional components to intestinal mucosal mucin, improve bioavailability, and effectively alleviate the formation of tear marks in pets. Wheat germ agglutinin can consume the carboxyl groups of polyacrylic acid, avoid the carboxyl groups of polyacrylic acid from being protonated in the gastric acid environment, and lose the ability to bind to mucin. In addition, zinc ions and chitosan have antibacterial and fresh-keeping effects, improve the quality of functional foods, and effectively alleviate the formation of tear marks in pets.

[0033] (5) In the technical solution of the present invention, the prepared pet functional food has a meat-like and relatively fragrant taste, good palatability, strong stability, balanced nutrition, easy digestion and absorption, and can clear away heat and reduce internal heat. It can effectively alleviate the formation of tear marks on pets. Long-term use can also improve the gloss of pets' fur, making it bright, smooth and soft. DETAILED DESCRIPTION

[0034] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0035] The raw materials used in the examples of the present invention are as follows, and all reagents used are of analytical grade.

[0036] Wherein, starch is selected from corn starch, and vegetable oil is selected from olive oil.

[0037] The molecular weight of carboxymethyl chitosan is 9500.

[0038] Wheat germ agglutinin product number is S25064, Shanghai Yuanye Biotechnology Co., Ltd.

[0039] Marigold extract was purchased from Xi'an Wanlv Biotechnology Co., Ltd., with a lutein content of 10%; wolfberry extract was purchased from Xi'an Tianbao Biotechnology Co., Ltd., with a wolfberry polysaccharide content of 50%; Cassia seed extract was purchased from Xi'an Tianbao Biotechnology Co., Ltd.

[0040] Example 1

[0041] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 40 parts chicken breast, 10 parts fish, 20 parts corn starch, 30 parts nutritional ingredients, 6 parts olive oil, 3 parts carrots, and 1 part salt;

[0042] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0043] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0044] S2. The mixture was extruded through an extruder, dried, dried at a temperature of 35 ° C, dried for 6 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0045] The extrusion device is a twin-screw extruder with an extrusion temperature of 70°C and a rotation speed of 270 r / min.

[0046] The nutritional components are specifically prepared by the following steps:

[0047] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.2:10, and the plant extract is composed of a marigold extract, a wolfberry extract, and a cassia seed extract in a mass ratio of 1:1:1.5;

[0048] A2. Carboxymethyl chitosan and arginine were added to a 0.5 mol / L acetic acid solution, stirred uniformly, and the extract was added and stirred for 30 min to obtain an aqueous phase with a mass ratio of carboxymethyl chitosan, arginine, acetic acid solution, and extract of 5:2:80:8. Liquid paraffin and sorbitan monooleate were mixed and stirred uniformly to obtain an oil phase with a volume ratio of liquid paraffin to sorbitan monooleate of 90:0.5. The aqueous phase was added to the oil phase and emulsified at 35°C for 1.5 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 3 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres with a volume ratio of aqueous phase to oil phase of 85:55.

[0049] A3. Add polyacrylic acid and wheat germ agglutinin to deionized water, stir evenly, and stir in a 25°C water bath for 2 hours to obtain a mixture; the mass ratio of polyacrylic acid, wheat germ agglutinin, and deionized water is 2.4:2:45;

[0050] A4. Chitosan microspheres and zinc chloride were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain a nutrient component. The mass ratio of chitosan microspheres, zinc chloride, deionized water, and the mixture was 5:1:55:28.

[0051] Example 2

[0052] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 45 parts chicken breast, 13 parts fish, 23 parts corn starch, 35 parts nutritional ingredients, 7 parts olive oil, 4 parts carrots, and 1.5 parts salt;

[0053] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0054] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0055] S2. The mixture was extruded through an extruder, dried at a drying temperature of 40°C for 7 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0056] The extrusion device is a twin-screw extruder with an extrusion temperature of 75°C and a rotation speed of 280 r / min.

[0057] The nutritional components are specifically prepared by the following steps:

[0058] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.3:11, and the plant extract is composed of a marigold extract, a wolfberry extract, and a cassia seed extract in a mass ratio of 1:1:1.7;

[0059] A2. Carboxymethyl chitosan and arginine were added to a 0.5 mol / L acetic acid solution, stirred, the extract was added, and stirring was continued for 30 min to obtain an aqueous phase in which the mass ratio of carboxymethyl chitosan, arginine, acetic acid solution, and extract was 5.5:2.1:83:9; liquid paraffin and sorbitan monooleate were mixed and stirred to obtain an oil phase in which the volume ratio of liquid paraffin and sorbitan monooleate was 100:0.7; the aqueous phase was added to the oil phase and emulsified at 40°C for 2 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 4 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres in a volume ratio of 90:57 between the aqueous phase and the oil phase.

[0060] A3. Add polyacrylic acid and wheat germ agglutinin to deionized water, stir evenly, and stir in a 25°C water bath for 2 hours to obtain a mixture; the mass ratio of polyacrylic acid, wheat germ agglutinin, and deionized water is 2.5:2.1:50;

[0061] A4. Chitosan microspheres and zinc chloride were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain a nutrient component. The mass ratio of chitosan microspheres, zinc chloride, deionized water, and the mixture was 5.5:1.5:60:30.

[0062] Example 3

[0063] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 50 parts chicken breast, 15 parts fish, 25 parts corn starch, 40 parts nutritional ingredients, 8 parts olive oil, 5 parts carrots, and 2 parts salt;

[0064] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0065] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0066] S2. The mixture was extruded through an extruder, dried at a drying temperature of 45 ° C for 8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0067] The extrusion device is a twin-screw extruder with an extrusion temperature of 80°C and a rotation speed of 290 r / min.

[0068] The nutritional components are specifically prepared by the following steps:

[0069] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.4:12, and the plant extract is composed of a marigold extract, a wolfberry extract, and a cassia seed extract in a mass ratio of 1:1:2;

[0070] A2. Carboxymethyl chitosan and arginine were added to a 0.5 mol / L acetic acid solution, stirred, the extract was added, and stirring was continued for 30 min to obtain an aqueous phase in which the mass ratio of carboxymethyl chitosan, arginine, acetic acid solution, and extract was 6:2.2:85:10; liquid paraffin and sorbitan monooleate were mixed and stirred to obtain an oil phase in which the volume ratio of liquid paraffin and sorbitan monooleate was 110:1; the aqueous phase was added to the oil phase and emulsified at 45°C for 2.5 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 5 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres in a volume ratio of 95:60 between the aqueous phase and the oil phase.

[0071] A3. Polyacrylic acid and wheat germ agglutinin were added to deionized water, stirred, and then stirred in a 25°C water bath for 2 h to obtain a mixture; the mass ratio of polyacrylic acid, wheat germ agglutinin, and deionized water was 2.6:2.2:55;

[0072] A4. Chitosan microspheres and zinc chloride were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain a nutrient component. The mass ratio of chitosan microspheres, zinc chloride, deionized water, and the mixture was 5.6:2:65:32.

[0073] Comparative Example 1

[0074] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 50 parts chicken breast, 15 parts fish, 25 parts corn starch, 40 parts nutritional ingredients, 8 parts olive oil, 5 parts carrots, and 2 parts salt;

[0075] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0076] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0077] S2. The mixture was extruded through an extruder, dried at a drying temperature of 45 ° C for 8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0078] The extrusion device is a twin-screw extruder with an extrusion temperature of 80°C and a rotation speed of 290 r / min.

[0079] The nutritional components are specifically prepared by the following steps:

[0080] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.4:12, and the plant extract is composed of a mixture of wolfberry extract and cassia seed extract in a mass ratio of 2:2;

[0081] A2. Carboxymethyl chitosan and arginine were added to a 0.5 mol / L acetic acid solution, stirred, the extract was added, and stirring was continued for 30 min to obtain an aqueous phase in which the mass ratio of carboxymethyl chitosan, arginine, acetic acid solution, and extract was 6:2.2:85:10; liquid paraffin and sorbitan monooleate were mixed and stirred to obtain an oil phase in which the volume ratio of liquid paraffin and sorbitan monooleate was 110:1; the aqueous phase was added to the oil phase and emulsified at 45°C for 2.5 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 5 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres in a volume ratio of 95:60 between the aqueous phase and the oil phase.

[0082] A3. Polyacrylic acid and wheat germ agglutinin were added to deionized water, stirred, and then stirred in a 25°C water bath for 2 h to obtain a mixture; the mass ratio of polyacrylic acid, wheat germ agglutinin, and deionized water was 2.6:2.2:55;

[0083] A4. Chitosan microspheres and zinc chloride were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain a nutrient component. The mass ratio of chitosan microspheres, zinc chloride, deionized water, and the mixture was 5.6:2:65:32.

[0084] Comparative Example 2

[0085] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 50 parts chicken breast, 15 parts fish, 25 parts corn starch, 40 parts nutritional ingredients, 8 parts olive oil, 5 parts carrots, and 2 parts salt;

[0086] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0087] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0088] S2. The mixture was extruded through an extruder, dried at a drying temperature of 45 ° C for 8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0089] The extrusion device is a twin-screw extruder with an extrusion temperature of 80°C and a rotation speed of 290 r / min.

[0090] The nutritional components are specifically prepared by the following steps:

[0091] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.4:12, and the plant extract is composed of a marigold extract and a cassia seed extract in a mass ratio of 2:2;

[0092] A2. Carboxymethyl chitosan and arginine were added to a 0.5 mol / L acetic acid solution, stirred, the extract was added, and stirring was continued for 30 min to obtain an aqueous phase in which the mass ratio of carboxymethyl chitosan, arginine, acetic acid solution, and extract was 6:2.2:85:10; liquid paraffin and sorbitan monooleate were mixed and stirred to obtain an oil phase in which the volume ratio of liquid paraffin and sorbitan monooleate was 110:1; the aqueous phase was added to the oil phase and emulsified at 45°C for 2.5 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 5 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres in a volume ratio of 95:60 between the aqueous phase and the oil phase.

[0093] A3. Polyacrylic acid and wheat germ agglutinin were added to deionized water, stirred, and then stirred in a 25°C water bath for 2 h to obtain a mixture; the mass ratio of polyacrylic acid, wheat germ agglutinin, and deionized water was 2.6:2.2:55;

[0094] A4. Chitosan microspheres and zinc chloride were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain a nutrient component. The mass ratio of chitosan microspheres, zinc chloride, deionized water, and the mixture was 5.6:2:65:32.

[0095] Comparative Example 3

[0096] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 50 parts chicken breast, 15 parts fish, 25 parts corn starch, 40 parts nutritional ingredients, 8 parts olive oil, 5 parts carrots, and 2 parts salt;

[0097] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0098] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0099] S2. The mixture was extruded through an extruder, dried at a drying temperature of 45 ° C for 8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0100] The extrusion device is a twin-screw extruder with an extrusion temperature of 80°C and a rotation speed of 290 r / min.

[0101] The nutritional components are specifically prepared by the following steps:

[0102] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.4:12, and the plant extract is composed of a marigold extract and a wolfberry extract in a mass ratio of 1:3;

[0103] A2. Carboxymethyl chitosan and arginine were added to a 0.5 mol / L acetic acid solution, stirred, the extract was added, and stirring was continued for 30 min to obtain an aqueous phase in which the mass ratio of carboxymethyl chitosan, arginine, acetic acid solution, and extract was 6:2.2:85:10; liquid paraffin and sorbitan monooleate were mixed and stirred to obtain an oil phase in which the volume ratio of liquid paraffin and sorbitan monooleate was 110:1; the aqueous phase was added to the oil phase and emulsified at 45°C for 2.5 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 5 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres in a volume ratio of 95:60 between the aqueous phase and the oil phase.

[0104] A3. Polyacrylic acid and wheat germ agglutinin were added to deionized water, stirred, and then stirred in a 25°C water bath for 2 h to obtain a mixture; the mass ratio of polyacrylic acid, wheat germ agglutinin, and deionized water was 2.6:2.2:55;

[0105] A4. Chitosan microspheres and zinc chloride were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain a nutrient component. The mass ratio of chitosan microspheres, zinc chloride, deionized water, and the mixture was 5.6:2:65:32.

[0106] Comparative Example 4

[0107] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 50 parts chicken breast, 15 parts fish, 25 parts corn starch, 40 parts nutritional ingredients, 8 parts olive oil, 5 parts carrots, and 2 parts salt;

[0108] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0109] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0110] S2. The mixture was extruded through an extruder, dried at a drying temperature of 45 ° C for 8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0111] The extrusion device is a twin-screw extruder with an extrusion temperature of 80°C and a rotation speed of 290 r / min.

[0112] The nutritional components are specifically prepared by the following steps:

[0113] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.4:12, and the plant extract is composed of a marigold extract, a wolfberry extract, and a cassia seed extract in a mass ratio of 1:1:2;

[0114] A2. Carboxymethyl chitosan was added to a 0.5 mol / L acetic acid solution, stirred, the extract was added, and stirring was continued for 30 min to obtain an aqueous phase in which the mass ratio of carboxymethyl chitosan, acetic acid solution, and extract was 8.2:85:10; liquid paraffin and sorbitan monooleate were mixed and stirred to obtain an oil phase in which the volume ratio of liquid paraffin and sorbitan monooleate was 110:1; the aqueous phase was added to the oil phase and emulsified at 45°C for 2.5 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 5 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres in a volume ratio of 95:60 between the aqueous phase and the oil phase.

[0115] A3. Polyacrylic acid and wheat germ agglutinin were added to deionized water, stirred, and then stirred in a 25°C water bath for 2 h to obtain a mixture; the mass ratio of polyacrylic acid, wheat germ agglutinin, and deionized water was 2.6:2.2:55;

[0116] A4. Chitosan microspheres and zinc chloride were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain a nutrient component. The mass ratio of chitosan microspheres, zinc chloride, deionized water, and the mixture was 5.6:2:65:32.

[0117] Comparative Example 5

[0118] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 50 parts chicken breast, 15 parts fish, 25 parts corn starch, 40 parts nutritional ingredients, 8 parts olive oil, 5 parts carrots, and 2 parts salt;

[0119] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0120] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0121] S2. The mixture was extruded through an extruder, dried at a drying temperature of 45 ° C for 8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0122] The extrusion device is a twin-screw extruder with an extrusion temperature of 80°C and a rotation speed of 290 r / min.

[0123] The nutritional components are specifically prepared by the following steps:

[0124] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.4:12, and the plant extract is composed of a marigold extract, a wolfberry extract, and a cassia seed extract in a mass ratio of 1:1:2;

[0125] A2. Carboxymethyl chitosan and arginine were added to a 0.5 mol / L acetic acid solution, stirred, the extract was added, and stirring was continued for 30 min to obtain an aqueous phase in which the mass ratio of carboxymethyl chitosan, arginine, acetic acid solution, and extract was 6:2.2:85:10; liquid paraffin and sorbitan monooleate were mixed and stirred to obtain an oil phase in which the volume ratio of liquid paraffin and sorbitan monooleate was 110:1; the aqueous phase was added to the oil phase and emulsified at 45°C for 2.5 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 5 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres in a volume ratio of 95:60 between the aqueous phase and the oil phase.

[0126] A3. Add polyacrylic acid to deionized water, stir, and continue stirring in a 25°C water bath for 2 hours to obtain a mixture; the mass ratio of polyacrylic acid to deionized water is 4.8:55;

[0127] A4. Chitosan microspheres and zinc chloride were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain a nutrient component. The mass ratio of chitosan microspheres, zinc chloride, deionized water, and the mixture was 5.6:2:65:32.

[0128] Comparative Example 6

[0129] A functional food for treating tear stains in pets, comprising the following ingredients in parts by weight: 50 parts chicken breast, 15 parts fish, 25 parts corn starch, 40 parts nutritional ingredients, 8 parts olive oil, 5 parts carrots, and 2 parts salt;

[0130] A method for preparing a functional food for treating pet tear stains comprises the following steps:

[0131] S1. Add chicken breast, fish, corn starch, nutrients, olive oil, carrots and salt to a chopper and mix well to obtain a mixture;

[0132] S2. The mixture was extruded through an extruder, dried at a drying temperature of 45 ° C for 8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food;

[0133] The extrusion device is a twin-screw extruder with an extrusion temperature of 80°C and a rotation speed of 290 r / min.

[0134] The nutritional components are specifically prepared by the following steps:

[0135] A1. The extract is composed of a mixture of plant extract and ethanol in a mass ratio of 2.4:12, and the plant extract is composed of a marigold extract, a wolfberry extract, and a cassia seed extract in a mass ratio of 1:1:2;

[0136] A2. Carboxymethyl chitosan and arginine were added to a 0.5 mol / L acetic acid solution, stirred, the extract was added, and stirring was continued for 30 min to obtain an aqueous phase in which the mass ratio of carboxymethyl chitosan, arginine, acetic acid solution, and extract was 6:2.2:85:10; liquid paraffin and sorbitan monooleate were mixed and stirred to obtain an oil phase in which the volume ratio of liquid paraffin and sorbitan monooleate was 110:1; the aqueous phase was added to the oil phase and emulsified at 45°C for 2.5 h. 1.1 g of glutaraldehyde was added and the reaction was stirred for 5 h. The mixture was cooled to room temperature and the product was collected by centrifugation. The product was washed three times with ethanol and dried in an oven at 60°C for 15 min to obtain chitosan microspheres in a volume ratio of 95:60 between the aqueous phase and the oil phase.

[0137] A3. Polyacrylic acid and wheat germ agglutinin were added to deionized water, stirred, and then stirred in a 25°C water bath for 2 h to obtain a mixture; the mass ratio of polyacrylic acid, wheat germ agglutinin, and deionized water was 2.6:2.2:55;

[0138] A4. Chitosan microspheres were added to deionized water and stirred at 40°C for 30 minutes. The mixture was then added and stirred at 60°C for 1 hour. The mixture was cooled to room temperature, filtered, washed three times with deionized water, and dried in an oven at 60°C for 10 minutes to obtain the nutrient component. The mass ratio of chitosan microspheres, deionized water, and the mixture was 5.6:65:32.

[0139] The performance of the functional foods prepared in Examples 1-3 and Comparative Examples 1-6 was tested.

[0140] 120 12-month-old golden retrievers with tear marks were selected. Clinical examination showed that the golden retrievers had no inverted eyelashes, nasolacrimal duct hypoplasia or blockage, and were fed in a standard manner. They were respectively divided into a control group, an embodiment 1-3 group, and a comparative example 1-6 group. The blank control group only consumed a basal diet (the formula of the basal diet was: 35% corn, 18% soybean meal, 25% flour, and 22% rice). The embodiment 1-3 group and the comparative example 1-6 group consumed a basal diet and functional food, with the functional food accounting for 1.5%.

[0141] Feeding and management: The dogs were raised in indoor cages with good ventilation and a room temperature of 25°C. Each dog was housed in a 0.5m×0.6m×0.6m cage. During the experiment, the dogs were fed three times a day, morning, noon, and evening, and had free access to water. They were dewormed and cleaned normally. The dogs were regularly tethered outside the kennel for appropriate exercise. The experimental period was 28 days.

[0142] Observe the condition of the golden retriever's eye mucus and smoothness. The smoothness is judged by the consistency of the coat's appearance and direction and the feel of the coat. It is measured on a 10-point scale. The stronger the feel, the shinier the coat, and the smoother it is, the higher the score.

[0143] Tear stain evaluation: The tear stains of the test dogs were scored in a 7-day cycle. Tear stains were divided into 5 levels, and the number of golden retrievers that eliminated tear stains in each cycle was recorded.

[0144] Tear stains are divided into 5 levels, as follows:

[0145] Level 1: Only the hair near the inner corner of the eye changes color, the color is lighter, and the texture is dry or moist;

[0146] Level 2: The hair changes color linearly from the inner corner of the eye to the corner of the mouth, with a range of less than 1 cm, and the color is pink, light brown, dark brown or dark brown, and the texture is dry or moist;

[0147] Level 3: linear discoloration range is larger than 1 cm, the color is darker, and the texture is moist;

[0148] Level 4: Not only is there linear discoloration, but the hair on the lower eyelid from the inner corner to the outer corner of the eye also changes color to a "crescent" shape, with a darker color and moist texture;

[0149] Level 5 is linear discoloration and a wider range of discoloration of the lower eyelid hair, appearing like "panda eyes". Even the hair in the triangular area surrounded by the inner corner of the eye, the outer corner of the eye and the corner of the mouth changes color. The color is darker, the texture is moist, and there may be an unpleasant odor.

[0150] The test results are shown in Table 1 below.

[0151] Table 1 Performance test of functional foods prepared in Examples 1-3 and Comparative Examples 1-6

[0152]

[0153] It can be seen from the data in Table 1 that the functional foods prepared in Examples 1-3 can significantly eliminate pet tear marks.

[0154] Comparative Example 1 replaces the mass of marigold extract with wolfberry extract, Comparative Example 2 replaces the mass of wolfberry extract with marigold extract, and Comparative Example 3 replaces the mass of cassia seed extract with wolfberry extract. The nutritional components prepared are added to functional foods, and their efficacy in alleviating pet tear marks decreases, proving that the mixed ratio of the three plant extracts has a synergistic effect in inhibiting the formation of pet tear marks, and the encapsulation of the plant extracts in carboxymethyl chitosan microspheres can improve the stability of the plant extracts.

[0155] In Comparative Example 4, arginine and other components are replaced with nutritional components prepared by carboxymethyl chitosan and added to functional foods. The efficacy of relieving pet tear marks is reduced, which proves that arginine can also cross-link with carboxymethyl chitosan microspheres to improve the mechanical strength and chemical stability of the microspheres and avoid leakage of active components. Arginine can also promote the construction of the pet's intestinal mucosal immune barrier, maintain the balance of normal intestinal flora and probiotics, reduce food allergy intake, reduce the irritation of eye secretions, and effectively relieve the formation of pet tear marks.

[0156] In Comparative Example 5, the nutrient components prepared by replacing wheat germ agglutinin with polyacrylic acid were added to the functional food. The efficacy of relieving pet tear stains decreased, proving that wheat germ agglutinin synergizes with polyacrylic acid to enhance the binding ability of the nutrient components to intestinal mucosal mucin and improve bioavailability. In addition, wheat germ agglutinin can consume the carboxyl groups of polyacrylic acid, preventing the carboxyl groups of polyacrylic acid from being protonated in the gastric acid environment and losing the ability to bind to mucin.

[0157] In Comparative Example 6, when the nutritional components prepared without zinc chloride were added to the functional food, the efficacy of relieving pet tear marks decreased, proving that polyacrylic acid and wheat germ agglutinin were deposited on the surface of carboxymethyl chitosan microspheres through zinc ions, thereby improving bioavailability and effectively relieving the formation of pet tear marks.

[0158] Throughout the specification, references to terms such as "one embodiment," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0159] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the described specific embodiments or replace them in similar ways. As long as they do not deviate from the invention or exceed the scope defined by the claims, they should all fall within the scope of protection of the present invention.

Claims

1. A functional food for solving pet tear stains, characterized in that: The invention comprises the following raw materials in parts by weight: 40-50 parts of chicken breast, 10-15 parts of fish, 20-25 parts of starch, 30-40 parts of nutritional components, 6-8 parts of vegetable oil, 3-5 parts of carrots and 1-2 parts of salt.

2. The functional food for solving pet tear stains according to claim 1, characterized in that: The nutritional components are specifically prepared by the following steps: A1. Carboxymethyl chitosan and arginine were added to an acetic acid solution and stirred uniformly. The extract was then added and stirred continuously to obtain an aqueous phase. Liquid paraffin and sorbitan monooleate were mixed and stirred uniformly to obtain an oil phase. The aqueous phase was added to the oil phase and emulsified at 35-45°C for 1.5-2.5 hours. Glutaraldehyde was added and the reaction was stirred for 3-5 hours. The mixture was cooled to room temperature, centrifuged, washed, and dried to obtain chitosan microspheres. A2. Add polyacrylic acid and wheat germ agglutinin to deionized water, stir well, place in a water bath, and continue stirring to obtain a mixture; A3. Add chitosan microspheres and zinc chloride to deionized water, stir evenly, add the mixture, continue stirring, cool to room temperature, filter, wash, and dry to obtain the nutritional components.

3. The functional food for solving pet tear stains according to claim 2, characterized in that: In step A1, the mass ratio of the carboxymethyl chitosan, arginine, acetic acid solution and extract is (5-6):(2-2.2):(80-85):(8-10); The volume ratio of the liquid paraffin and sorbitan monooleate is (90-110):(0.5-1); The volume ratio of the water phase to the oil phase is (85-95):(55-60).

4. The functional food for treating pet tear stains according to claim 2, characterized in that: In step A1, the extract is prepared by mixing the plant extract and ethanol in a mass ratio of (2.2-2.4):(10-12).

5. The functional food for treating pet tear stains according to claim 4, characterized in that: The plant extract is prepared by mixing marigold extract, wolfberry fruit extract and cassia seed extract in a mass ratio of 1:1:(1.5-2).

6. The functional food for treating pet tear stains according to claim 2, characterized in that: In step A2, the mass ratio of the polyacrylic acid, wheat germ agglutinin and deionized water is (2.4-2.6):(2-2.2):(45-55).

7. The functional food for treating pet tear stains according to claim 2, characterized in that: In step A3, the mass ratio of the chitosan microspheres, zinc chloride, deionized water and the mixture is (5-5.6):(1-2):(55-65):(28-32).

8. The functional food for treating pet tear stains according to claim 1, characterized in that: The starch is selected from any one of corn starch, rice starch, potato starch and tapioca starch.

9. The functional food for treating pet tear stains according to claim 1, characterized in that: The vegetable oil is selected from any one of olive oil, coconut oil, soybean oil, rapeseed oil, sunflower oil and linseed oil.

10. A method for preparing the functional food for solving pet tear stains according to any one of claims 1 to 9, characterized in that: The method comprises the following preparation steps: S1. Add chicken breast, fish, starch, nutrients, vegetable oil, carrots and salt to a chopper and mix well to obtain a mixture; S2. The mixture is extruded through an extruder, dried at a temperature of 35-45° C. for 6-8 hours, cooled to room temperature, sterilized, and cut to obtain a functional food.

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