Preparation method and application of immunity-enhancing fertilizer containing vitamins and glucose

Abstract

The invention relates to the field of fertilizer preparation, and particularly discloses a preparation method of a vitamin and glucose-containing immunity-enhancing fertilizer, which comprises the following steps: S1, premixing a fermented organic carrier and polyglutamic acid at 45-50 DEG C according to a mass ratio of (7-9): 1 for 30-45 minutes to form a porous base material; s2, premixing vitamin C phosphate and a seaweed extract according to a mass ratio of 1: 3, and alternately coating the surface of the vitamin D3 microcapsule with 3-5 layers of protective films by adopting a fluidized bed coating technology; through molecular-level complexation of the vitamin B complex and the glucose derivative, endogenous disease-resistant signal channels of plants can be activated, and systematicness of immune response is improved; a porous structure is constructed through cooperation of a fermented organic carrier and polyglutamic acid, the water and fertilizer retention capacity of soil can be enhanced, and targeted absorption of nutrients by a root system is promoted; through root perfusion and directional release, collaborative delivery of the immune inducer and trace elements is realized, the infection path of pathogenic bacteria is effectively blocked, and the incidence rate of diseases is reduced.

Description

Technical Field

[0001] The invention belongs to the field of fertilizer preparation, in particular to a method for preparing fertilizer containing vitamins and glucose for enhancing immunity and application thereof. Background Art

[0002] Fertilizer preparation refers to the process of converting various raw materials into fertilizer products that provide plant nutrients through specific processes and methods. This process aims to increase the nutrient content, improve its physical properties, enhance its efficiency, and meet the needs of different crops and soils.

[0003] Traditional disease-resistant fertilizers rely on chemical pesticides or single immune inducers, which can easily induce drug resistance in pathogens and lack the ability to synergistically activate endogenous signaling pathways in plants. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a method for preparing a fertilizer containing vitamins and glucose to enhance immunity, so as to solve the problem that traditional disease-resistant fertilizers in the prior art rely on chemical pesticides or single immune inducers, easily induce drug resistance of pathogens, and lack the ability to synergistically activate endogenous signal pathways of plants.

[0005] A method for preparing a fertilizer containing vitamins and glucose for enhancing immunity comprises the following steps:

[0006] S1. Premixing the fermentation organic carrier and polyglutamic acid at a mass ratio of 7 to 9:1 at 45 to 50° C. for 30 to 45 minutes to form a porous base material;

[0007] S2. After premixing vitamin C phosphate and seaweed extract in a mass ratio of 1:3, 3 to 5 layers of protective film are alternately coated on the surface of the vitamin D3 microcapsules using a fluidized bed coating technique. The mass ratio of the vitamin D3 microcapsules to the coating material is 1:0.8-1.2.

[0008] S3, performing a low-temperature chelation reaction of humic acid-chelated trace elements and glucose derivatives at a molar ratio of 1:2-1:3 at pH 5.8-6.3 and 35-40° C. for 2-3 hours;

[0009] S4, placing the coated product obtained in step S2 and the chelated product obtained in step S3 in a mass ratio of 1.5:1-2:1 in an ultrasonic reactor, and coupling at a frequency of 20-40 kHz for 15-30 minutes;

[0010] S5. Place the base material of step S1, the coupling product obtained in step S4, the vitamin B complex and the plant immunity inducer in a mass ratio of 60-70:15-20:8-12:2-3 in a three-dimensional mixer, and mix at a speed of 10-15 rpm for 1-2 hours under a nitrogen atmosphere.

[0011] Preferably, in step S1, the fermentation organic carrier is prepared by the following process:

[0012] S11, mixing soybean meal, yeast fermentation broth, and Trichoderma metabolites in a mass ratio of 3:1:0.5;

[0013] S12, solid-state fermentation at 45°C for 72 hours, turning the compost every 12 hours;

[0014] S13, drying the fermentation product at 60° C. to a moisture content of ≤8%, and crushing the product through an 80-mesh sieve.

[0015] Preferably, in step S2, the protective film comprises a double-layer structure with an inner layer of hydroxypropyl methylcellulose and an outer layer of glyceryl monostearate, and the thickness ratio of the inner layer to the outer layer is 1:0.8; the fluidized bed coating process parameters include: inlet air temperature 45-50°C, atomization pressure 0.3-0.5MPa, and coating weight gain rate 8-12%; the particle size of the vitamin D3 microcapsules is 50-80μm.

[0016] Preferably, in step S3, the low-temperature chelation treatment adopts a three-stage pH adjustment process, specifically comprising:

[0017] Phase 1: Maintain at pH 6.3 for 15 minutes;

[0018] Phase 2: Rapidly adjust to pH 5.8 and maintain for 20 minutes;

[0019] Stage 3: Adjust back to pH 6.1 and maintain for 25 minutes;

[0020] The glucose derivative is a mixture of zinc gluconate and ferrous gluconate in a ratio of 2 to 3.

[0021] Preferably, in step S4, the ultrasonic coupling process is implemented in two stages, including:

[0022] Phase 1: 20kHz frequency treatment for 10 minutes, power density 0.5W / cm 3 ;

[0023] Phase 2: Switch to 40kHz frequency for 10-20 minutes, power density 1.2W / cm 3 ;

[0024] A 0.2-0.3 MPa nitrogen pulse purge was performed between the two-stage treatments, with a duration of 30-60 seconds.

[0025] Preferably, in step S3, the humic acid chelated trace elements include zinc, iron, and manganese, with a molar ratio of Zn:Fe:Mn=1:0.6-0.7:0.4-0.5, a chelation degree ≥92%, and a free ion content ≤0.5ppm.

[0026] Preferably, in step S5, the vitamin B complex consists of thiamine nitrate, riboflavin-5-sodium phosphate, and pyridoxine hydrochloride in a ratio of 40-45%: 25-30%: 20-25%.

[0027] Preferably, the purity of thiamine nitrate, riboflavin-5-sodium phosphate, and pyridoxine hydrochloride in the vitamin B complex is ≥99.5%, and they form a complex with a molecular weight of 1500-3000 Da with the glucose derivative.

[0028] The invention relates to an application of the fertilizer prepared by the above method in improving the disease resistance of crops. The fertilizer is applied by foliar spraying or root irrigation, with an application concentration of 0.5 to 1.5 g / L and an application interval of 15 to 20 days.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] Through the molecular complexation of vitamin B complexes and glucose derivatives, it can activate the plant's endogenous disease resistance signaling pathways and enhance the systemic immune response;

[0031] By synergistically constructing a porous structure through fermentation of organic carriers and polyglutamic acid, the soil's ability to retain water and fertilizer can be enhanced, promoting targeted nutrient absorption by the roots.

[0032] Through targeted release through root irrigation, the immune inducer and trace elements are delivered in a coordinated manner, effectively blocking the infection pathway of pathogens and reducing the incidence of diseases;

[0033] By matching the humic acid chelation system with the plant metabolic rhythm, nutrient waste is avoided and nutrient utilization efficiency is improved;

[0034] By combining alternating coating technology with low-temperature chelation process, the stability of active ingredients is significantly enhanced, avoiding the degradation of vitamins and trace elements caused by environmental factors such as light and soil pH. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a schematic diagram of the preparation process of the present invention. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] like Figure 1 As shown:

[0038] Example 1:

[0039] Step S1: Porous base material preparation

[0040] Raw material ratio: fermentation organic carrier: polyglutamic acid = 7:1

[0041] Process parameters: premix at 45°C for 30 minutes

[0042] Preparation of fermentation organic carrier:

[0043] Soybean meal, yeast fermentation broth, and Trichoderma metabolites were mixed at a ratio of 3:1:0.5.

[0044] Solid-state fermentation at 45°C for 72 hours (turning the compost every 12 hours)

[0045] Dry at 60℃ to 8% moisture content, crush through 80 mesh sieve

[0046] Step S2: Vitamin D3 coating

[0047] Coating material ratio: Vitamin C phosphate: seaweed extract = 1:3

[0048] Number of coating layers: 3 layers alternating

[0049] Protective film structure:

[0050] Inner layer of hydroxypropyl methylcellulose (55% thickness)

[0051] Outer layer: Glyceryl monostearate (45% thickness)

[0052] Process parameters:

[0053] Inlet air temperature 45℃, atomization pressure 0.3MPa

[0054] Coating weight gain rate 8%, microcapsule particle size 50μm

[0055] Step S3: Low temperature chelation reaction

[0056] Raw material ratio: Humic acid chelated trace elements: glucose derivatives = 1:2 (molar ratio)

[0057] Glucose derivative compound: zinc gluconate: ferrous gluconate = 2:1

[0058] pH adjustment process:

[0059] The first stage is pH 6.3, maintained for 15 minutes

[0060] The second stage is pH 5.8, maintained for 20 minutes

[0061] The third stage is pH 6.1, maintained for 25 minutes

[0062] Trace element ratio: Zn:Fe:Mn=1:0.6:0.4

[0063] Step S4: Ultrasonic coupling

[0064] Raw material ratio: coated product: chelated product = 1.5:1

[0065] Process parameters:

[0066] Stage 1: 20kHz frequency (0.5W / cm 3 ) Process for 10 minutes

[0067] Nitrogen pulse purge 0.2MPa for 30 seconds

[0068] Second stage: 40kHz frequency (1.2W / cm 3 ) Process for 10 minutes

[0069] Step S5: Three-dimensional mixing

[0070] Raw material ratio: base material: coupling product: vitamin B group: immune inducer = 60:15:8:2

[0071] Vitamin B complex composition: Thiamine nitrate 45%, Riboflavin-5-sodium phosphate 30%, Pyridoxine hydrochloride 25%

[0072] Process parameters: nitrogen atmosphere, 10 rpm mixing for 1 hour

[0073] Example 2:

[0074] Step S1: Porous base material preparation

[0075] Raw material ratio: fermentation organic carrier: polyglutamic acid = 8:1

[0076] Process parameters: 47.5℃ premixing for 37.5 minutes

[0077] Preparation of fermentation organic carrier: same as Example 1

[0078] Step S2: Vitamin D3 coating

[0079] Number of coating layers: 4 layers alternating

[0080] Process parameters:

[0081] Inlet air temperature 47.5℃, atomization pressure 0.4MPa

[0082] Coating weight gain rate 10%, microcapsule particle size 65μm

[0083] Step S3: Low temperature chelation reaction

[0084] Raw material ratio: Humic acid chelated trace elements: glucose derivatives = 1:2.5 (molar ratio)

[0085] Glucose derivative compound: zinc gluconate: ferrous gluconate = 2.5:1

[0086] Trace element ratio: Zn:Fe:Mn=1:0.65:0.45

[0087] Step S4: Ultrasonic coupling

[0088] Raw material ratio: coated product: chelated product = 1.75:1

[0089] Process parameters:

[0090] Phase 1: 20kHz frequency treatment for 10 minutes

[0091] Nitrogen pulse purge 0.25MPa for 45 seconds

[0092] Phase 2: 40kHz frequency treatment for 15 minutes

[0093] Step S5: Three-dimensional mixing

[0094] Raw material ratio: base material: coupling product: vitamin B group: immune inducer = 65:17.5:10:2.5

[0095] Vitamin B complex composition: Thiamine nitrate 42.5%, Riboflavin-5-sodium phosphate 27.5%, Pyridoxine hydrochloride 22.5%

[0096] Process parameters: 12.5 rpm mixing for 1.5 hours

[0097] Example 3:

[0098] Step S1: Porous base material preparation

[0099] Raw material ratio: fermentation organic carrier: polyglutamic acid = 9:1

[0100] Process parameters: premixing at 50°C for 45 minutes

[0101] Preparation of fermentation organic carrier: same as Example 1

[0102] Step S2: Vitamin D3 coating

[0103] Number of coating layers: 5 layers alternating

[0104] Process parameters:

[0105] Inlet air temperature 50℃, atomization pressure 0.5MPa

[0106] Coating weight gain rate 12%, microcapsule particle size 80μm

[0107] Step S3: Low temperature chelation reaction

[0108] Raw material ratio: Humic acid chelated trace elements: glucose derivatives = 1:3 (molar ratio)

[0109] Glucose derivative compound: zinc gluconate: ferrous gluconate = 3:1

[0110] Trace element ratio: Zn:Fe:Mn=1:0.7:0.5

[0111] Step S4: Ultrasonic coupling

[0112] Raw material ratio: coated product: chelated product = 2:1

[0113] Process parameters:

[0114] Phase 1: 20kHz frequency treatment for 10 minutes

[0115] Nitrogen pulse purge 0.3MPa for 60 seconds

[0116] Phase 2: 40kHz frequency treatment for 20 minutes

[0117] Step S5: Three-dimensional mixing

[0118] Raw material ratio: base material: coupling product: vitamin B group: immune inducer = 70:20:12:3

[0119] Vitamin B complex: Thiamine nitrate 40%, Riboflavin-5-phosphate sodium 25%, Pyridoxine hydrochloride 20%

[0120] Process parameters: mixing at 15 rpm for 2 hours.

[0121] Experimental example:

[0122] Experimental purpose: To verify the technical advantages of the present invention in terms of slow-release performance, nutrient synergy and plant immunity enhancement, and to compare the differences in effects under conditions of traditional fertilizers and process deficiencies.

[0123] Experimental period: May 1st to August 31st (a complete crop growth cycle).

[0124] Reference group: Example 2 of the present invention.

[0125] Comparative design:

[0126] Comparative Example A: Commercially available slow-release fertilizer (containing vitamin D3, without glucose derivatives and coating process).

[0127] Comparative Example B: Step S4 (ultrasonic coupling treatment) was omitted, and the rest was the same as in Example 2.

[0128] Comparative Example C: Ordinary humic acid (without chelated trace elements) was used to replace the product of step S3.

[0129] Comparative Example D: No plant immunity inducer was added, and the rest was the same as Example 2.

[0130] Experimental implementation steps

[0131] Material preparation:

[0132] The reference group and comparative example fertilizers were prepared as required, and a certain brand of slow-release fertilizer was selected as the commercially available fertilizer.

[0133] Field trials:

[0134] Crop: Tomato (variety "Ruby"), 3 replicates per treatment group, each plot area 20 m2.

[0135] Fertilization plan: Apply base fertilizer at 150kg / hectare and topdressing at intervals of 30 days.

[0136] Detection indicators:

[0137] Nutrient slow-release performance: Soil samples were collected weekly to test the residual levels of vitamin D3 and trace elements (Zn, Fe, and Mn).

[0138] Plant physiological indicators: plant height, leaf chlorophyll content (SPAD value), and fruit vitamin C content.

[0139] Disease resistance: Artificial inoculation with gray mold fungus (Botrytis cinerea) was performed, and the percentage of diseased area was calculated.

[0140] Soil health: microbial diversity (16SrRNA sequencing), organic matter content.

[0141] Experimental data and result analysis

[0142] Experimental Form

[0143]

[0144] Core advantage verification and mechanism analysis

[0145] Advantages of sustained-release performance (compared with comparative examples A and B)

[0146] Data difference: The sustained-release period of vitamin D3 in Example 2 is extended by 68% compared with that in Comparative Example A and by 29% compared with that in Comparative Example B.

[0147] Mechanism verification:

[0148] Coating process: SEM showed that the surface of the microcapsules of Example 2 was a dense honeycomb structure (pore size ≤ 5 μm), while that of Comparative Example A was a loose layered structure (pore size > 20 μm).

[0149] Ultrasonic coupling: XRD analysis shows that the order of the chelate crystals in Example 2 is increased by 40%, reducing ion loss in the soil.

[0150] Nutrient synergy (compared with comparative examples C and D)

[0151] Data differences: The chlorophyll content of the leaves of Example 2 is increased by 21.9% compared with that of Comparative Example C, and the vitamin C content of the fruit is increased by 5.2% compared with that of Comparative Example D.

[0152] Chemical mechanism:

[0153] Synergy of immune inducers: ELISA test showed that the activation intensity of the jasmonic acid (JA) signaling pathway in the plants of Example 2 was 2.3 times higher than that in the control example D.

[0154] Improved soil health

[0155] Microbial diversity: In Example 2, the Actinobacteria accounted for 28.5% of the soil, an increase of 87% compared to Comparative Example A (15.2%), promoting the degradation of organic matter.

[0156] Organic matter accumulation: The organic matter content of the soil in Example 2 was 52% higher than that in Comparative Example A, which was attributed to the nutrient adsorption by the porous structure of the fermented organic carrier.

[0157] Application Example 1 (Tomato Planting: Medium Concentration + Standard Interval)

[0158] Application Scenario

[0159] Crop: Tomato (variety "Ruby"), greenhouse cultivation, soil pH 6.5, 3 years of continuous cropping, high incidence of gray mold.

[0160] Application parameters:

[0161] Root drip irrigation

[0162] Concentration: 1.0 g / L (fertilizer prepared in Example 2)

[0163] Application amount: 500mL / plant

[0164] Nodes: Seedling stage, flowering stage, fruit setting stage, fruit expansion stage (15-day interval)

[0165] Foliar spray (staggered with root application for 7 days)

[0166] Concentration: 0.3g / L+0.2% lecithin enhancer

[0167] Spraying time: early flowering stage, young fruit stage

[0168] Key points:

[0169] Use atomizing nozzle (droplet size 80-100μm)

[0170] Focus on spraying the back of leaves (coverage ≥ 90%)

[0171] Method: Root drip irrigation, 500 mL per plant each time, for a total of 4 times (seedling stage, flowering stage, fruit setting stage, and fruit swelling stage).

[0172] Effect evaluation

[0173] index Administration group Control group (ordinary fertilizer) Gray mold incidence 8.2％ 36.5％ Single fruit weight (g) 185±12 142±15 Vitamin C content (mg / 100g) 32.1±1.8 24.6±2.1 Soil actinomycete abundance 28.5％ 15.2％

[0174] Conclusion: The disease resistance rate increased by 77.5%, which was attributed to the activation of tomato JA signaling pathway by the immune inducer in the fertilizer (JA content increased by 2.1 times as detected by ELISA).

[0175] The fruit quality was significantly improved, which was related to the synergistic effect of the vitamin B complex and the glucose derivative in Example 2.

[0176] Application Example 2 (Strawberry Planting: High Concentration + Long Interval)

[0177] Application Scenario

[0178] Crop: Strawberry (variety "Akira"), grown outdoors, with heavy clay soil and frequent powdery mildew.

[0179] Application parameters:

[0180] Root irrigation

[0181] Concentration: 1.5g / L

[0182] Application amount: 50kg / mu

[0183] Nodes: after planting, budding period, before harvesting

[0184] Foliar spray

[0185] Concentration: 0.4g / L+5mmol / L potassium silicate

[0186] Spraying time: budding stage, young fruit stage, 10 days before harvest

[0187] Effect evaluation

[0188] index Administration group Control group (commercially available slow-release fertilizer) Powdery mildew disease index 12.3 47.8 Soluble solids (%) 9.8±0.5 7.2±0.6 Yield (kg / mu) 2850±120 2100±150 Soil organic matter (%) 3.6±0.2 2.3±0.3

[0189] Conclusion: High concentration application (1.5 g / L) can prolong the nutrient release period (20 days) and reduce the infection window period of powdery mildew spores.

[0190] Soil organic matter increased by 56.5%, verifying the contribution of the porous structure of the fermented organic carrier to soil improvement.

[0191] Application Example 3 (Cucumber Planting: Low Concentration + Short Interval)

[0192] Application Scenario

[0193] Crop: Cucumber (variety "Jinyou 35"), grown in greenhouse, soil salinization, severe target spot disease.

[0194] Application parameters:

[0195] Root micro-sprinkler irrigation

[0196] Concentration: 0.5g / L

[0197] Application amount: 300mL / plant

[0198] Node: Seedling stage to harvest stage (every 15 days)

[0199] Foliar emergency spray

[0200] Concentration: 0.25g / L+0.1% polyglycerol fatty acid ester

[0201] Triggering conditions: within 24 hours of the onset of illness

[0202] Effect evaluation

[0203] index Administration group Control group (no fertilizer) Target spot disease lesion area ratio 5.7％ 41.2％ Melon strip straightness (qualified rate) 92％ 68％ Zinc absorption (mg / kg) 18.3±1.5 9.6±1.2 Soil EC value (mS / cm) 1.8±0.1 3.5±0.3

[0204] Conclusion: Low-concentration and high-frequency application significantly reduced soil salinity (EC value decreased by 48.6%), and humic acid-chelated zinc promoted the thickening of cucumber cell walls (microscopic observation thickness +23%), thus resisting the invasion of pathogens.

[0205] The straightness of the melon strips increased by 35.3%, which was related to the improvement of the nutrient transport efficiency of the vessels by humic acid-ferrous gluconate in Example 2.

[0206] Application Example 4 (Grape Planting: Foliar Spraying + Root Coordination)

[0207] Application Scenario

[0208] Crop: Grapes (variety "Xiahei"), cultivated in a rain-sheltered environment, for 5 consecutive years, soil pH 5.8, magnesium deficiency and high incidence of downy mildew.

[0209] Core issue: Soil acidification leads to obstructed root absorption, and traditional foliar fertilizers have a short effective period (<7 days), making it difficult to prevent and control systemic infection of downy mildew.

[0210] Application parameters

[0211] Foliar spray:

[0212] Concentration: 0.3 g / L (Example 2 fertilizer + 0.2% Tween 20 surfactant)

[0213] Spraying time: new shoot growth period, flower spike separation period, early color change period, spray twice in each period (5 days apart)

[0214] Operation requirements: droplet size 50-80μm, leaf back coverage rate of more than 90%

[0215] Root Synergy:

[0216] Drip irrigation supplement: 0.5g / L concentration, 1L per plant (staggered with foliar spraying for 3 days)

[0217] Effect evaluation

[0218] index Administration group Control group (conventional foliar fertilizer) Downy mildew inhibition rate 91.5％ 52.3％ Soluble sugar (%) 18.7±0.6 14.2±0.8 Single ear weight (g) 650±25 520±30 Leaf magnesium content (mg / kg) 2850±150 1800±200 Soil pH recovery 6.2±0.1 (after 6 months) 5.7±0.2

[0219] Conclusion and mechanism

[0220] Disease prevention and control breakthroughs:

[0221] Foliar spraying is quickly absorbed through the stomata, activating the salicylic acid (SA) signaling pathway within 6 hours (qPCR detection shows a 3.8-fold increase in PR1 gene expression), and forming local immune memory.

[0222] In conjunction with root drip irrigation, it induced systemic resistance (SAR), reducing the expansion rate of downy mildew hyphae by 72%.

[0223] Quality and soil improvement:

[0224] Magnesium gluconate complex is directly absorbed through the leaves, with a magnesium utilization rate of 85% (traditional soil application is only 35%), promoting the transport of sugar to the fruit.

[0225] Polyglutamic acid carrier adsorbs soil H + , the pH increased from 5.8 to 6.2 within 6 months, alleviating acidification disorder.

[0226] Technical advantages:

[0227] The foliage-root dual-channel control system reduces pesticide usage by 60%, and optimizes the sugar-acid ratio to 25:1 (the industry standard for high-quality fruit is ≥20:1).

[0228] Vitamin C phosphate delays the photolysis of foliar fertilizers, extending the effective period to 10-12 days (conventional products 5-7 days).

[0229] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0230] In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0231] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for preparing a fertilizer containing vitamins and glucose for enhancing immunity, characterized in that: The following steps are involved: S1. Premixing the fermentation organic carrier and polyglutamic acid at a mass ratio of 7 to 9:1 at 45 to 50° C. for 30 to 45 minutes to form a porous base material; S2. After premixing vitamin C phosphate and seaweed extract in a mass ratio of 1:3, 3 to 5 layers of protective film are alternately coated on the surface of the vitamin D3 microcapsules using a fluidized bed coating technique. The mass ratio of the vitamin D3 microcapsules to the coating material is 1:0.8-1.

2. S3, performing a low-temperature chelation reaction of humic acid-chelated trace elements and glucose derivatives at a molar ratio of 1:2-1:3 at pH 5.8-6.3 and 35-40° C. for 2-3 hours; S4, placing the coated product obtained in step S2 and the chelated product obtained in step S3 in a mass ratio of 1.5:1-2:1 in an ultrasonic reactor, and coupling at a frequency of 20-40 kHz for 15-30 minutes; S5. Place the base material of step S1, the coupling product obtained in step S4, the vitamin B complex and the plant immunity inducer in a mass ratio of 60-70:15-20:8-12:2-3 in a three-dimensional mixer, and mix at a speed of 10-15 rpm for 1-2 hours under a nitrogen atmosphere.

2. The method for preparing a fertilizer containing vitamins and glucose for enhancing immunity according to claim 1, wherein: In step S1, the fermentation organic carrier is prepared by the following process: S11, mixing soybean meal, yeast fermentation broth, and Trichoderma metabolites in a mass ratio of 3:1:0.5; S12, solid-state fermentation at 45°C for 72 hours, turning the compost every 12 hours; S13, drying the fermentation product at 60° C. to a moisture content of ≤8%, and crushing the product through an 80-mesh sieve.

3. The method for preparing a fertilizer containing vitamins and glucose for enhancing immunity as claimed in claim 2, characterized in that: In step S2, the protective film comprises a double-layer structure with an inner layer of hydroxypropyl methylcellulose and an outer layer of glyceryl monostearate, and the thickness ratio of the inner layer to the outer layer is 1:0.8; the fluidized bed coating process parameters include: inlet air temperature 45-50°C, atomization pressure 0.3-0.5MPa, and coating weight gain rate 8-12%; the particle size of the vitamin D3 microcapsules is 50-80μm.

4. The method for preparing a fertilizer containing vitamins and glucose for enhancing immunity according to claim 1, wherein: In step S3, the low-temperature chelation treatment adopts a three-stage pH adjustment process, specifically comprising: Phase 1: Maintain at pH 6.3 for 15 minutes; Phase 2: Rapidly adjust to pH 5.8 and maintain for 20 minutes; Stage 3: Adjust back to pH 6.1 and maintain for 25 minutes; The glucose derivative is a mixture of zinc gluconate and ferrous gluconate in a ratio of 2 to 3.

5. The method for preparing a fertilizer containing vitamins and glucose for enhancing immunity according to claim 1, characterized in that: In step S4, the ultrasonic coupling process is implemented in two stages, including: Phase 1: 20kHz frequency treatment for 10 minutes, power density 0.5W / cm 3 ; Phase 2: Switch to 40kHz frequency for 10-20 minutes, power density 1.2W / cm 3 ; A 0.2-0.3 MPa nitrogen pulse purge was performed between the two-stage treatments, with a duration of 30-60 seconds.

6. The method for preparing a fertilizer containing vitamins and glucose for enhancing immunity according to claim 1, characterized in that: In step S3, the humic acid chelated trace elements include zinc, iron, and manganese, with a molar ratio of Zn:Fe:Mn=1:0.6-0.7:0.4-0.5, a chelation degree ≥92%, and a free ion content ≤0.5ppm.

7. The method for preparing a fertilizer containing vitamins and glucose for enhancing immunity according to claim 1, characterized in that: In step S5, the vitamin B complex is composed of thiamine nitrate, riboflavin-5-sodium phosphate, and pyridoxine hydrochloride in a ratio of 40-45%: 25-30%: 20-25%.

8. The method for preparing a fertilizer containing vitamins and glucose for enhancing immunity according to claim 7, characterized in that: The purity of thiamine nitrate, riboflavin-5-sodium phosphate and pyridoxine hydrochloride in the vitamin B complex is greater than or equal to 99.5%, and the complex with the glucose derivative forms a complex with a molecular weight of 1500-3000Da.

9. Use of a fertilizer prepared by the method according to any one of claims 1 to 8 in improving the disease resistance of crops, characterized in that: The fertilizer is applied by foliar spraying or root irrigation, with an application concentration of 0.5-1.5 g / L and an application interval of 15-20 days.

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