Preparation method and application of Chinese herbal medicine extract fertilizer for enhancing immunity

Abstract

The invention relates to the technical field of agricultural biology, and particularly discloses a preparation method and application of a Chinese herbal medicine extract fertilizer for enhancing immunity, the preparation method comprises the following steps: S1, performing water extraction and alcohol precipitation extraction on Chinese herbal medicine raw materials to obtain a Chinese herbal medicine extracting solution and medicine residues; s2, crushing the obtained medicine residues, inoculating white-rot fungi or bacillus subtilis, and performing solid-state fermentation at the fermentation temperature of 25-35 DEG C for 5-7 days to obtain a fermentation product; s3, concentrating and drying the obtained Chinese herbal medicine extracting solution to obtain Chinese herbal medicine extract powder; s4, the obtained Chinese herbal medicine extract powder is mixed with humic acid, ferric salt or zinc salt is added, stirring reaction is conducted under the condition that the pH ranges from 5.0 to 6.5, and a Chinese herbal medicine-humic acid metal chelate is formed; chinese herbal medicine compound extraction and microbial solid fermentation are combined, and traditional waste medicine residues are converted into high-activity organic fertilizer carriers, so that efficient utilization of resources is realized, meanwhile, the immune induction function of the fertilizer is enhanced, and the disease resistance of crops is remarkably improved.

Description

Technical Field

[0001] The invention belongs to the field of agricultural biotechnology, and particularly relates to a preparation method and application of a Chinese herbal medicine extract fertilizer for enhancing immunity. Background Art

[0002] In the field of agricultural biotechnology, the research, development, and application of fertilizers have always been the key to improving crop yields and quality and promoting sustainable agricultural development. In recent years, with people's growing concern about food safety and health, traditional chemical fertilizers have been controversial due to the potential environmental pollution and soil degradation problems they may cause. Against this background, new fertilizers based on natural resources and with eco-friendly properties have emerged. Among them, Chinese herbal extract fertilizers have attracted much attention because of their combined nutritional supplementation and biological regulation functions.

[0003] Chinese herbal medicine extract fertilizers extract active ingredients from Chinese herbal medicines, such as polysaccharides, flavonoids, alkaloids, etc., and use these active ingredients to regulate crop growth and enhance immunity, thereby improving crop disease resistance and increasing yield. However, most of the Chinese herbal medicine extract fertilizers currently on the market have problems such as complex preparation process, high cost, low active ingredient content, poor stability and low utilization efficiency. In particular, during the preparation process, the extraction of active ingredients from Chinese herbal raw materials is often not sufficient, and the extracted residues are often treated as waste, which not only causes a waste of resources, but may also burden the environment. In addition, after application, the active ingredients of existing Chinese herbal medicine extract fertilizers are easily affected by environmental factors and degraded, resulting in a short duration of fertilizer effect, which is difficult to meet the needs of the entire crop growth cycle. Therefore, staff need to improve them. Summary of the Invention

[0004] The purpose of the present invention is to provide a preparation method and application of a Chinese herbal medicine extract fertilizer for enhancing immunity, so as to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A method for preparing a Chinese herbal medicine extract fertilizer for enhancing immunity comprises the following steps:

[0007] S1, extracting the Chinese herbal medicine raw materials with water and alcohol to obtain the Chinese herbal medicine extract and medicinal residue;

[0008] S2. Grinding the obtained medicinal residue, inoculating white rot fungi or Bacillus subtilis for solid-state fermentation at a temperature of 25-35° C. for 5-7 days to obtain a fermentation product;

[0009] S3, concentrating and drying the obtained Chinese herbal medicine extract to obtain Chinese herbal medicine extract powder;

[0010] S4, mixing the obtained Chinese herbal medicine extract powder with humic acid, adding iron salt or zinc salt, stirring and reacting at pH 5.0-6.5 to form a Chinese herbal medicine-humic acid metal chelate;

[0011] S5. Mixing the obtained fermentation product, the obtained Chinese herbal medicine-humic acid metal chelate and zein, spray-drying the mixture to form embedded particles, and obtaining a fertilizer.

[0012] Preferably, in step S1, the Chinese herbal medicine raw materials include astragalus, honeysuckle, rhubarb, liquorice and mugwort, with a mass ratio of 30:20:15:20:15.

[0013] Preferably, in step S2, the inoculum amount of the solid-state fermentation is 5% to 10% of the mass of the medicinal residue, and the humidity is maintained at 60% to 70% during the fermentation process.

[0014] Preferably, in step S4, the mass ratio of the Chinese herbal medicine extract powder to humic acid is 1:3-1:5, the iron salt is ferrous sulfate, and the zinc salt is zinc sulfate.

[0015] Preferably, in step S5, the amount of zein used is 10% to 15% of the total mass of the fermentation product and the Chinese herbal medicine-humic acid metal chelate.

[0016] A Chinese herbal medicine extract fertilizer for enhancing immunity, comprising fermented medicinal residues, a Chinese herbal medicine-humic acid metal chelate and a zein embedding layer.

[0017] Preferably, the content of active ingredients of Chinese herbal medicine in the fertilizer is ≥5%, and the content of humic acid is ≥20%.

[0018] The invention discloses an application of a Chinese herbal medicine extract fertilizer for enhancing immunity. The fertilizer is used for enhancing crop immunity, and the application methods include basal application, topdressing or foliar spraying.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) By combining the extraction of Chinese herbal medicine compounds with microbial solid-state fermentation, traditional waste medicinal residues are converted into highly active organic fertilizer carriers, thereby achieving efficient resource utilization. At the same time, the immune induction function of the fertilizer is enhanced, significantly improving the disease resistance of crops.

[0021] (2) Through the metal ion chelation technology of Chinese herbal medicine active ingredients and humic acid, a stable "immune-nutrition" complex is formed, thereby improving the bioavailability of trace elements. It not only supplements the nutrients such as iron and zinc required by crops, but also activates the plant's systemic resistance (SAR) through Chinese herbal medicine ingredients, achieving the dual effects of disease resistance and nutrition.

[0022] (3) By encapsulating active ingredients in natural degradable materials (such as zein and lipid bilayers), pH-responsive sustained release is achieved, thereby extending the duration of action of active ingredients in Chinese herbal medicines, reducing losses caused by rainwater erosion or photolysis, ensuring that fertilizers continue to function during the critical growth period of crops, and improving utilization rates. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a production flow chart of the present invention. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Example 1:

[0026] See also Figure 1 As shown, the preparation and application of the fertilizer of compound Chinese herbal extract of Astragalus and Honeysuckle

[0027] Raw material preparation

[0028] Chinese herbal medicine raw materials: Astragalus (30%), honeysuckle (20%), rhubarb (15%), liquorice (20%), mugwort (15%), all of which were dried and crushed through a 40-mesh sieve.

[0029] Microbial strain: white rot fungus (Phanerochaete chrysosporium CICC 40719), activated and prepared into spore suspension (1×10 6 CFU / mL).

[0030] Other excipients: humic acid (humic acid content ≥ 60%), ferrous sulfate (FeSO4·7H2O), zein (purity ≥ 90%).

[0031] Chinese herbal medicine extraction

[0032] Water extraction: Add deionized water to the mixed Chinese herbal medicine powder at a solid-liquid ratio of 1:10, reflux at 80°C for 2 hours, and filter; add water to the residue again at a ratio of 1:8 for 1 hour, and combine the two filtrates.

[0033] Alcohol precipitation: The filtrate was concentrated to 1 / 5 of the original volume, 95% ethanol was added to a final concentration of 60%, and the mixture was allowed to stand at 4°C for 12 hours. The supernatant was collected by centrifugation (4000 rpm, 15 minutes), and concentrated under reduced pressure to an extract. The extract powder was dried under vacuum at 60°C (yield 18.5%).

[0034] Solid-state fermentation of medicinal residues

[0035] Fermentation matrix: The pH of the extracted medicinal residue (water content 55%) was adjusted to 6.0, and a white rot fungus spore suspension was inoculated at an 8% inoculum rate.

[0036] Fermentation conditions: 28°C, 65% humidity for 6 days, turning the compost every 24 hours. The fermentation product was dried at 60°C and crushed to obtain dark brown baking powder (cellulose degradation rate 42%).

[0037] Preparation of Chinese herbal medicine-humic acid chelated iron

[0038] Chelation reaction: 10 g of the extract powder was mixed with 40 g of humic acid, 15 g of ferrous sulfate was added, the pH was adjusted to 5.5 with citric acid, and the mixture was stirred at 50°C for 2 hours.

[0039] Purification: The reaction solution was centrifuged to remove insoluble matter and spray-dried to obtain a reddish-brown chelate powder (iron content 12.3%).

[0040] Preparation of slow-release embedded fertilizer

[0041] Embedding process: 50g of baking powder, 30g of chelate and 12g of zein were mixed, granulated with 20% ethanol solution, and dried in a fluidized bed at 40°C into 1-2mm particles.

[0042] Sustained release test: In pH 5.0 buffer, the release rate is ≤30% in 24 hours and the cumulative release is ≥80% in 72 hours.

[0043] Field application effect

[0044] Crop: Tomato (variety "Zhongshu No. 5").

[0045] Application method: base application (25kg / mu) + foliar spraying during flowering period (800 times liquid, 2 times).

[0046] Effect:

[0047] The incidence of gray mold was reduced by 63% (the control was conventional fertilizer);

[0048] The vitamin C content of the fruit increases by 22%.

[0049] Example 2:

[0050] See also Figure 1 As shown, the preparation and application of Ginkgo biloba and Rhubarb compound Chinese herbal extract fertilizer

[0051] Raw material preparation

[0052] Chinese herbal medicine raw materials: Ginkgo biloba leaves (40%), rhubarb (30%), liquorice (30%), crushed and passed through a 60-mesh sieve.

[0053] Microbial strain: Bacillus subtilis CGMCC 1.3358, cultured in liquid until OD 600 =1.0.

[0054] Other excipients: humic acid, zinc sulfate (ZnSO4·7H2O), lipid bilayer (soy lecithin: cholesterol = 7:3).

[0055] Chinese herbal medicine extraction

[0056] Enzyme-assisted extraction: Chinese herbal medicine powder was added to 0.1 M citric acid buffer (pH 4.5) at a ratio of 1:12, cellulase (5 U / g) and pectinase (3 U / g) were added, and enzymatic hydrolysis was performed at 50°C for 3 hours. Subsequent water extraction and alcohol precipitation were the same as in Example 1 (total flavonoid yield 2.8%).

[0057] Solid-state fermentation of medicinal residues

[0058] Fermentation medium: The medicinal residue was inoculated with Bacillus subtilis (10% v / w) and fermented at 37°C for 5 days with 10 minutes of ventilation every day. The β-glucan content in the fermentation product increased to 9.7%.

[0059] Preparation of Chinese Herbal Medicine-Humic Acid Chelated Zinc

[0060] Chelation reaction: 15 g of the extract powder was reacted with 50 g of humic acid and 20 g of zinc sulfate at pH 6.0 for 3 hours, and the mixture was freeze-dried to obtain a chelate (zinc content 8.5%).

[0061] Preparation of lipid bilayer-encapsulated fertilizers

[0062] Embedding process: 40g of baking powder, 35g of chelate and 25g of lipid bilayer (dissolved in chloroform and then evaporated into film) were mixed, homogenized under high pressure and then spray-dried.

[0063] Slow-release performance: Under simulated rhizosphere pH 6.5 conditions, the release rate is 45% within 48 hours.

[0064] Application effect (rice)

[0065] Application method: base application (30kg / mu) + spraying during tillering period (600 times liquid).

[0066] Effect:

[0067] Rice blast resistance increased by 57%;

[0068] The zinc content of the grains reached 28.6 mg / kg (the control was 18.2 mg / kg).

[0069] Example 3:

[0070] See also Figure 1 As shown, the preparation and application of compound Chinese herbal extract fertilizer of Artemisia argyi and Tripterygium wilfordii

[0071] Raw materials and special treatments

[0072] Chinese herbal medicine raw materials: mugwort leaf (70%), tripterygium wilfordii (30%), tripterygium wilfordii is detoxified by steaming (100° C. for 1 hour) and then crushed.

[0073] Microorganisms: Aspergillus niger solid-state fermentation produces citric acid to adjust pH.

[0074] Extraction and fermentation

[0075] Extraction: The volatile oil of Artemisia argyi was extracted by supercritical CO2 (yield 1.2%), and the residue was mixed with Tripterygium wilfordii and extracted with water.

[0076] Fermentation: The medicinal residue was inoculated with Aspergillus niger (5%) and fermented at 30°C for 7 days. The citric acid content in the fermentation product reached 11.3%.

[0077] Humic acid chelation and encapsulation

[0078] Chelation: The extract is mixed with humic acid in a ratio of 1:4 to chelate copper ions (CuSO4) and react at pH 4.0.

[0079] Embedment: Sodium alginate-chitosan microencapsulation (particle size 50-100 μm) is used.

[0080] Application (grape downy mildew control)

[0081] Application: Foliar spray (1000 times dilution, 3 times with 7 days interval).

[0082] Effect:

[0083] 81% protection against downy mildew (compared to 65% for the control agent);

[0084] The anthocyanin content of the fruit increases by 35%.

[0085] Comparative Example:

[0086] The following three examples are compared with existing conventional fertilizers and commercially available Chinese herbal fertilizers, and are analyzed in detail from the aspects of immune enhancement effect, fertilizer utilization rate, crop yield and quality.

[0087] Example 1 (Astragalus and Honeysuckle Compound Fertilizer) Comparison with Prior Art

[0088] Comparison objects:

[0089] Conventional fertilizer (NPK compound fertilizer, 15-15-15);

[0090] Commercially available Chinese herbal organic fertilizer (a certain brand of "plant immune activation fertilizer", the main ingredient is astragalus polysaccharide).

[0091] Comparative Experiment Design (Tomato Planting Experiment):

[0092] Treatment group 1: Example 1 fertilizer (basal application of 25 kg / mu + foliar spraying of 800 times dilution, twice);

[0093] Control group 1: conventional chemical fertilizer (basal application 30 kg / mu + topdressing urea 10 kg / mu);

[0094] Control group 2: commercially available Chinese herbal organic fertilizer (recommended dosage: 30 kg / mu).

[0095] Comparison results:

[0096] index Example 1 Conventional fertilizers Commercially available Chinese herbal fertilizer Gray mold incidence (%) 12.3 33.5 21.8 Leaf peroxidase (POD) activity (U / g) 45.6 28.2 36.4 Vitamin C content of fruit (mg / 100g) 32.5 26.7 29.1 Yield per plant (kg) 5.8 5.2 5.5

[0097] in conclusion:

[0098] Immune enhancement effect: The gray mold incidence rate of Example 1 was the lowest (12.3%), which was significantly better than conventional chemical fertilizers (33.5%) and commercially available Chinese herbal fertilizers (21.8%).

[0099] The POD enzyme activity was the highest (45.6 U / g), indicating that the fertilizer of the present invention can more effectively activate the plant disease resistance system.

[0100] Improved quality: Vitamin C content is 22% higher than conventional chemical fertilizers, and better than commercially available Chinese herbal fertilizers (11% increase).

[0101] Yield impact: The yield per plant is 11.5% higher than that of conventional chemical fertilizers, indicating that the present invention does not affect yield increase while resisting diseases.

[0102] Example 2 (Ginkgo biloba and rhubarb compound fertilizer) compared with prior art

[0103] Comparison objects:

[0104] Ordinary zinc fertilizer (zinc sulfate, ZnSO4);

[0105] Humic acid chelated zinc fertilizer (a certain brand of "humic acid zinc fertilizer").

[0106] Comparative Experiment Design (Rice Planting Experiment):

[0107] Treatment group 2: Example 2 fertilizer (basal application of 30 kg / mu + foliar spraying of 600 times dilution);

[0108] Control group 3: ordinary zinc sulfate (basal application 5kg / mu);

[0109] Control group 4: commercially available humic acid zinc fertilizer (recommended dosage: 20 kg / mu).

[0110] Comparison results:

[0111] index Example 2 Ordinary zinc sulfate Commercially available humic acid zinc fertilizer Rice blast incidence (%) 8.7 25.4 15.2 Root zinc content (mg / kg) 42.3 35.6 38.1 Zinc content in grains (mg / kg) 28.6 18.2 22.4 Thousand-grain weight (g) 26.5 24.8 25.3

[0112] in conclusion:

[0113] Comparison of disease resistance: The rice blast incidence rate of Example 2 was the lowest (8.7%), which was significantly better than that of ordinary zinc sulfate (25.4%) and commercially available humic acid zinc fertilizer (15.2%).

[0114] Zinc absorption efficiency: Root zinc content is 18.8% higher than that of ordinary zinc sulfate, indicating that Chinese herbal medicine and humic acid chelated zinc are more easily absorbed. Seed zinc content is 57% higher than that of ordinary zinc fertilizers, meeting the standards for zinc-rich rice.

[0115] Yield-increasing effect: 1000-grain weight is 6.9% higher than that of ordinary zinc fertilizer, indicating that this application promotes grain filling while resisting disease.

[0116] Example 3 (Artemisia argyi and Tripterygium wilfordii compound fertilizer) compared with prior art

[0117] Comparison objects:

[0118] Chemical fungicide (azoxystrobin, 25% SC);

[0119] Biopesticide (Bacillus subtilis preparation).

[0120] Comparative test design (grape downy mildew control test):

[0121] Treatment group 3: Example 3 fertilizer (1000 times dilution sprayed on leaves, 3 times);

[0122] Control group 5: chemical fungicide (azoxystrobin, sprayed at the recommended concentration);

[0123] Control group 6: Bacillus subtilis preparation (1×10 8 CFU / mL, sprayed 3 times).

[0124] Comparison results:

[0125] index Example 3 Chemical fungicides biological pesticides Downy mildew control effect (%) 81.2 85.3 62.7 Fruit anthocyanin content (mg / kg) 356 298 312 Pesticide residue testing Not detected Detection (0.02ppm) Not detected

[0126] in conclusion:

[0127] Disease prevention effect: The disease prevention effect of Example 3 (81.2%) is close to that of chemical fungicides (85.3%) and much higher than that of biological pesticides (62.7%).

[0128] Safety and quality: There is no pesticide residue, while trace amounts of chemical fungicide residues were detected. The anthocyanin content is 19.5% higher than that of chemical treatment, indicating that Chinese herbal extracts can improve fruit quality.

[0129] Comprehensive comparison conclusion

[0130] Immune-enhancing effect: The fertilizer of the present application is superior to conventional fertilizers and commercially available Chinese herbal fertilizers in preventing and controlling diseases such as gray mold, rice blast, and downy mildew, with the prevention effect increased by 30% to 60%.

[0131] Nutrition and quality: Chinese herbal medicine-humic acid chelation technology significantly improves the absorption of trace elements (such as zinc and iron), and increases nutrients such as vitamin C and anthocyanins by 15% to 20%, which is better than chemical fertilizers.

[0132] Environmental friendliness: No chemical pesticide residues, in line with organic farming standards, while chemical treatments have residual risks.

[0133] Final Recommendations

[0134] Example 1 is suitable for nightshade vegetables (such as tomatoes and peppers), taking into account both disease resistance and quality.

[0135] Example 2 is suitable for food crops such as rice and wheat, and can increase zinc content and resist rice blast.

[0136] Example 3 is suitable for economic crops such as grapes and strawberries, and can replace chemical fungicides.

[0137] Example 4:

[0138] Preparation and application of Chinese herbal medicine extract fertilizer for root application via drip irrigation

[0139] Raw material preparation

[0140] Chinese herbal medicine raw materials: Astragalus (35%), honeysuckle (20%), rhubarb (15%), liquorice (20%), mugwort (10%), all of which were dried and crushed through a 60-mesh sieve.

[0141] Microbial strain: Bacillus subtilis CGMCC 1.3358, cultured in liquid until OD 600 =1.0.

[0142] Other auxiliary materials: humic acid (humic acid content ≥ 60%), ferrous sulfate (FeSO4·7H2O), sodium alginate (viscosity ≥ 500mPa·s).

[0143] Extraction of Chinese herbal medicine and fermentation of medicinal residues

[0144] Water extraction and alcohol precipitation: The Chinese herbal medicine powder was extracted with water at a solid-liquid ratio of 1:10 (80°C, 2 hours), and the residue was extracted a second time (1:8, 1 hour). The combined filtrate was concentrated to 1 / 5 volume, precipitated with 60% ethanol, centrifuged, and the supernatant was dried to obtain the extract powder (yield 20.1%).

[0145] Solid-state fermentation: The medicinal residue was inoculated with Bacillus subtilis (8% inoculation amount) and fermented at 35°C and 65% humidity for 5 days. The β-glucan content in the fermentation product reached 8.9%.

[0146] Preparation of Chinese herbal medicine-humic acid chelated iron

[0147] 12 g of the extract powder was mixed with 45 g of humic acid, and 18 g of ferrous sulfate was added. The mixture was reacted at pH 5.5 for 2 hours, and spray-dried to obtain a chelate (iron content 11.8%).

[0148] Preparation of special fertilizer for drip irrigation

[0149] Recipe: Baking powder 40g, chelate 35g, sodium alginate 25g.

[0150] Process: After mixing, dissolve in water to form a viscous suspension (solid content 15%), and then granulate into 1-3mm sustained-release granules through extrusion.

[0151] Slow-release performance: In soil simulation liquid, the release rate is ≤50% within 48 hours.

[0152] Effect of drip irrigation (cucumber planting experiment)

[0153] Application method: drip irrigation after planting (diluted 500 times, once every 10 days, for a total of 3 times), with a dosage of 15 kg per mu each time.

[0154] Effect:

[0155] The incidence of root rot was reduced by 58% (the control was conventional compound fertilizer);

[0156] Root activity increased by 35% (measured by TTC method);

[0157] The yield per plant increased by 18% (6.2 kg / plant in the control group and 7.3 kg / plant in the treated group).

[0158] Comparative Analysis

[0159] index Example 4 (drip irrigation) Conventional drip irrigation fertilizer Root rot incidence (%) 9.5 22.7 Root activity (μgTTF / g / h) 45.3 33.6 Yield (kg / plant) 7.3 6.2

[0160] in conclusion:

[0161] Resistance to soil-borne diseases: Drip irrigation can significantly reduce the incidence of root rot, thanks to the root-directed release of active ingredients of Chinese herbal medicines.

[0162] Root-promoting effect: Sodium alginate slow-release particles promote root absorption and improve root activity.

[0163] Applicability: Suitable for facility agriculture (such as greenhouse cucumbers), solving the problem that traditional foliar spraying is not effective enough against root diseases.

[0164] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a Chinese herbal medicine extract fertilizer for enhancing immunity, characterized in that: The following steps are involved: S1, extracting the Chinese herbal medicine raw materials with water and alcohol to obtain the Chinese herbal medicine extract and medicinal residue; S2. crushing the obtained medicinal residue, inoculating one of white rot fungi and Bacillus subtilis to carry out solid-state fermentation at a fermentation temperature of 25-35° C. for 5-7 days to obtain a fermentation product; S3, concentrating and drying the obtained Chinese herbal medicine extract to obtain Chinese herbal medicine extract powder; S4, mixing the obtained Chinese herbal medicine extract powder with humic acid, adding one of an iron salt and a zinc salt, and stirring to react at a pH of 5.0-6.5 to form a Chinese herbal medicine-humic acid metal chelate; S5. Mixing the obtained fermentation product, the obtained Chinese herbal medicine-humic acid metal chelate and zein, spray-drying the mixture to form embedded particles, and obtaining a fertilizer.

2. The method for preparing the immunity-enhancing Chinese herbal medicine extract fertilizer according to claim 1, characterized in that: In step S1, the Chinese herbal medicine raw materials include astragalus, honeysuckle, rhubarb, liquorice and mugwort, with a mass ratio of 30:20:15:20:

15.

3. The method for preparing the immunity-enhancing Chinese herbal medicine extract fertilizer according to claim 1, characterized in that: In step S2, the inoculation amount of the solid-state fermentation is 5% to 10% of the mass of the medicinal residue, and the humidity is maintained at 60% to 70% during the fermentation process.

4. The method for preparing the immunity-enhancing Chinese herbal medicine extract fertilizer according to claim 1, characterized in that: In step S4, the mass ratio of the Chinese herbal medicine extract powder to humic acid is 1:3-1:5, the iron salt is ferrous sulfate, and the zinc salt is zinc sulfate.

5. The method for preparing the immunity-enhancing Chinese herbal medicine extract fertilizer according to claim 1, characterized in that: In step S5, the amount of zein used is 10% to 15% of the total mass of the fermentation product and the Chinese herbal medicine-humic acid metal chelate.

6. A Chinese herbal medicine extract fertilizer for enhancing immunity, suitable for the preparation method of a Chinese herbal medicine extract fertilizer for enhancing immunity according to any one of claims 1 to 5, characterized in that: The fertilizer comprises fermented medicinal residues, Chinese herbal medicine-humic acid metal chelate and a zein embedding layer.

7. The Chinese herbal medicine extract fertilizer for enhancing immunity according to claim 6, characterized in that: The content of active ingredients of Chinese herbal medicine in the fertilizer is ≥5%, and the content of humic acid is ≥20%.

8. An application of a Chinese herbal medicine extract fertilizer for enhancing immunity, applicable to the Chinese herbal medicine extract fertilizer for enhancing immunity according to any one of claims 6 to 7, characterized in that: The fertilizer is used to enhance crop immunity, and the application method includes one of base application, topdressing and foliar spraying.

Images