Pseudomonas oryzae and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas oryzae and application thereof. The invention aims to provide more effective biocontrol bacteria for preventing and treating rice blast. The technical scheme of the invention is as follows: the pseudomonas oryzae B35 is provided, and the preservation number of the pseudomonas oryzae B35 is GDMCC (China General Microbiological Culture Collection Center) No: 66230. According to the invention, a strain of pseudomonas oryzae is screened, has an obvious antagonistic effect on pyricularia oryzae, and can obviously reduce the pathogenesis level of rice blast; meanwhile, when the pseudomonas oryzae liquid is applied to root irrigation treatment of the rice, an obvious growth promoting effect on the rice is found.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and particularly relates to a strain of Pseudomonas oryzae and applications thereof. Background Art

[0002] Control measures for rice blast primarily include screening for resistant varieties and the use of fungicides. Screening for resistant varieties is currently the most cost-effective strategy for controlling rice blast. However, the complexity of the rice blast fungus allows it to rapidly mutate and adapt to new resistant rice plants, leading to a vulnerability to resistance loss. Fungicides can rapidly kill plant pathogens, but excessive and repeated application can lead to rapid mutations in pathogen populations, increasing resistance to fungicides. This can also cause residual toxicity in grains, induce pesticide resistance, reduce soil quality, and disrupt natural ecosystems.

[0003] Using beneficial microorganisms and their metabolites or plant-derived bioactive substances to control plant diseases is a sustainable, green approach. Numerous studies have demonstrated that many biocontrol fungi and bacteria can form strong symbiotic relationships with their host plants, promoting their growth and development, enhancing their tolerance to adverse environments, and improving their disease resistance. Biocontrol fungi primarily include yeasts (Saccharomyces), Trichoderma sp., Harpophora oryzae, and Piriform mosporaindica, while biocontrol bacteria primarily include Actinomycetes, Pseudomonas, Bacillus, and Agrobacterium. They all control plant diseases through various mechanisms, such as secreting antimicrobial substances, competing for nutrients and space, and inducing systemic resistance (ISR) in plants. Furthermore, some biocontrol fungi can form symbiotic relationships with plants, promoting their growth and development. Biocontrol fungi and biocontrol bacteria are both important biological control resources with broad application prospects. Summary of the Invention

[0004] The purpose of the invention is to provide more effective biocontrol bacteria for preventing and controlling rice blast.

[0005] The technical solution of the present invention is a Pseudomonas oryzihabitans B35 strain, with a preservation number of GDMCC No: 66230.

[0006] The present invention also provides the use of the Pseudomonas oryzae in preparing phosphate-dissolving, potassium-dissolving or / and nitrogen-fixing products.

[0007] The present invention also provides the use of the Pseudomonas oryzae in producing IAA and / or siderophore.

[0008] The present invention also provides application of the Pseudomonas oryzae in resisting rice blast.

[0009] The present invention also provides a product for dissolving phosphorus, dissolving potassium or / and fixing nitrogen, the main component of which is the Pseudomonas oryzae, the fermentation product of the Pseudomonas oryzae or / and the secretion of the Pseudomonas oryzae.

[0010] The present invention also provides a product containing IAA and / or siderophore, the main component of which is the Pseudomonas oryzae, the fermentation product of the Pseudomonas oryzae and / or the secretion of the Pseudomonas oryzae.

[0011] The present invention also provides a preparation for preventing and controlling rice blast, which contains the Pseudomonas oryzae, a fermentation product of the Pseudomonas oryzae and / or a secretion of the Pseudomonas oryzae.

[0012] Furthermore, the preparation also contains Bacillus altitudinis B08, whose deposit number is GDMCC No: 66286.

[0013] Beneficial effects of the present invention: Among the strains isolated from rice phyllosphere microorganisms, the present invention found a strain of Pseudomonas oryzae that had a significant antagonistic effect on rice blast fungi through plate antagonism experiments, rice detached leaf scratch experiments, and rice pot spraying experiments. It was found that the strain had a significant antagonistic effect on rice blast fungi and could significantly reduce the incidence of rice blast. At the same time, it was found that applying a bacterial solution of Pseudomonas oryzae to irrigate rice roots had a significant growth-promoting effect on rice. In addition, the isolated Pseudomonas oryzae was compounded with Bacillus subtilis and then subjected to rice detached leaf scratch experiments and rice pot spraying experiments. It was found that when the binary compound was used, the bacterial agent could significantly reduce the incidence of rice blast. It can be seen that the Pseudomonas oryzae screened by the present invention has a preventive and therapeutic effect on rice blast as a biocontrol bacterium, and when used as a composite bacterial agent with Bacillus subtilis, the preventive and therapeutic effect on rice blast is even more significant.

[0014] The Pseudomonas oryzihabitans B35 strain used in the present invention was deposited at the Institute of Microbiology, Guangdong Academy of Sciences on April 27, 2025, with a deposit number of GDMCC No: 66230; the deposit address is: Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou City, Guangdong Province; Postal Code: 510070.

[0015] The Bacillus altitudinis B08 strain used in the present invention was deposited at the Institute of Microbiology, Guangdong Academy of Sciences on June 6, 2025, with a deposit number of GDMCC No: 66286; the deposit address is: Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou City, Guangdong Province; Postal Code: 510070. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 , single-plant microbial resistance to rice blast antagonistic plate experiment.

[0017] Figure 2 , experimental results of single microorganisms and their combination to promote rice blast resistance in detached leaves.

[0018] Figure 3 Results of a potted experiment on promoting rice blast resistance using single microorganisms and their combination. Figure P131 is the blast fungus.

[0019] Figure 4 , Pseudomonas orvzihabitans (B35) has the ability to solubilize phosphorus, solubilize potassium, fix nitrogen and produce iron carriers.

[0020] Figure 5 , Pseudomonas orvzihabitans (B35) has the ability to produce IAA.

[0021] Figure 6 , experimental results of Pseudomonas orvzihabitans (B35) promoting rice seed germination.

[0022] Figure 7 , experimental results of Pseudomonas orvzihabitans (B35) promoting rice growth.

[0023] Figure 8 , sequence alignment of Pseudomonas orvzihabitans (B35).

[0024] Figure 9 , Bacillus altitudinis (B08) sequence alignment.

[0025] Figure 10 , Pseudomonas orvzihabitans (B35) and Bacillusaltitudinis (B08). DETAILED DESCRIPTION

[0026] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in conjunction with the accompanying drawings and preferred embodiments.

[0027] Example 1 Screening and Identification of Bacillus altitudinis (B08) and Pseudomonas orvzihabitans (B35) of the Present Invention Rice was planted in paddy fields where rice blast had been prevalent for many years. When the rice grew to 28 days old, the rice leaves were cut for microbial isolation. The rice leaves were rinsed with tap water, cut into small pieces, and then dried and surface disinfected in a sterile operating table: rinsed with 75% ethanol for 30 seconds and rinsed once with sterile water, then rinsed with 1% sodium hypochlorite for 30 seconds and rinsed three times with sterile water, and the surface moisture was absorbed with filter paper. 100 μL of the supernatant from the last sterile water wash was taken and spread on R2A and TSA culture media as blank controls. The leaves were cultured at 35 ℃ for 5-7 days. If no colony growth was observed, it indicated that the surface of the rice leaves was thoroughly disinfected. 0.2 g of the surface-sterilized rice leaves were placed in a mortar, and 2 mL of 10 mM MgCl2 solution was added to grind them completely. The supernatant was then diluted to 10% by gradient. -1 , 10 -2 , 10 -3 , 10 -4 Spread on R2A or TSA culture medium and culture at 35°C for 5-7 days. Pick out single colonies with different characteristics based on colony size, morphology, color, etc. (When selecting single colonies, try to choose different strains for purification and preservation based on characteristics such as morphology, size, color, and texture, rather than screening for a fixed one or several microorganisms.) After three generations of continuous purification, pick out single colonies and shake culture them in LB for 12 hours. Then, mix the bacterial solution with 50% glycerol in a 1:1 ratio and add it to the bacterial storage tube. Store at -80°C for later use.

[0028] Use an inoculation loop to dip the bacterial solution in the glycerol tube, activate B35 and B08 on the LB plate, and activate them for three generations in a row for later use. The rice blast plate antagonism experiment was used to screen bacterial strains with strong antagonism to rice blast, and the rice blast pathogen strain P131 (provided by Professor Peng Youliang of China Agricultural University) was used as an indicator bacteria to screen antagonistic strains. Use a 5mm diameter puncher to take the indicator bacteria cake and inoculate it in the center of the PDA culture medium plate. Use a toothpick to pick up the activated bacterial single colony, and draw a line about 2cm away from the cake. The plate with the line dipped in sterile water is used as the blank control group (CK), and the treatment is repeated three times. The plate was inverted and cultured at 28°C. When the diameter of the pathogenic fungus in the control group grew to about 3 / 4 of the diameter of the culture dish, the diameter of the pathogenic fungus was measured by the cross method, the antibacterial rate of the strain was calculated, and the strong antagonistic strain was screened. The experiment proved (Table 1 and Figure 1 Among the bacteria isolated from rice leaves, Bacillus altitudinis (B08) and Pseudomonas oryzae habitans (B35) showed significant antagonistic effects against rice blast fungi. B35 colonies were smaller, light yellow, and had a regular shape with regular and neat edges, a smooth surface, and a uniform texture. B08 colonies were relatively larger, milky white, and had irregular shapes with flat, diffuse edges and a raised surface with an uneven texture. Figure 10 ).

[0029] Table 1 Results of the single-plant microbial antagonism test against rice blast Molecular biological identification was performed on the screened bacterial strains B35 and B08, which demonstrated strong antagonism against rice blast fungus. A single colony was cultured in R2B medium at 28°C and 180 rpm for 24 hours. Six microliters of the bacterial suspension was then added to 10 microliters of buffer 1*ROMAN I and incubated at 95°C for 30 minutes in a thermal cycler. 10 microliters of buffer II was then added. This buffer II was used directly as a template in subsequent PCR reactions. The buffer consisted of 1*ROMAN I (25mM NaOH, 0.2mM EDTA, adjusted to pH 12); and buffer II (40mM Tris-HCl, pH 7.5). PCR amplification of the target fragment from the 16S rDNA gene was performed using primers 27F (SEQ ID No. 1, 5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (SEQ ID No. 2, 5'-GGTTACCTTGTTACGACTT-3'). PCR amplification conditions included pre-denaturation at 94°C for 5 min, 23 cycles of denaturation at 94°C for 30 sec, annealing at 55°C for 30 sec, extension at 72°C for 25 sec, and extension at 72°C for 8 min, followed by a stop at 8°C. PCR products were run on an electrophoretic gel and sent to Tianyi Biotechnology for sequencing and verification.

[0030] The 16S rDNA sequence of strain B35 (SEQ ID No. 3) was determined and submitted to the NCBI database for comparison. Fifteen model strains with 16S rDNA sequences showing at least 90% similarity across species were identified, and a phylogenetic tree was constructed using MEGA 7.0 software using the Neighbor-Joining method. The selected type strains were: Pseudomonas oryzihabitans LMG7040 (NR 117269.1), Pseudomonas flavocrustae EP178 (MG778852.2), Pseudomonas aestiva DGS32 (PQ037653.1), Pseudomonas stutzeri DSM 50238 (U26416.1), Pseudomonas paralcaligenes MRCP1333 (MT604974.1), Pseudomonas schmalbachii Milli4 (MW717294.1), Pseudomonas nitroreducens NBRC 12694 (NR 113601.1), Pseudomonas straminea JCM 2783 (LC420056.1), Pseudomonas crudilactis UCMA 17988 (MH016575.2), Pseudomonas putida IAM 1236 (NR 043424.1), Pseudomonas monteiliiCIP 104883 (NR 024910.1), Pseudomonas plecoglossicida NBRC 103162 (MH021884.1), Pseudomonas leptonychotis CCM 8849 (NR 180260.1), Marinobacterium stanieri ATCC27130 (NR 024699.1), Marinobacterium stanieri ATCC 27130 was an outgroup, and the results were shown in Figure 8 The phylogenetic tree showed that the strain B35 was most closely related to Pseudomonas oryzihabitans LMG7040.

[0031] The 16S rDNA sequence of strain B08 (SEQ ID No. 4) was submitted to the NCBI database for comparison. Fifteen model strains with 16S rDNA sequences from different species with at least 94% similarity were identified, and a phylogenetic tree was constructed using MEGA 7.0 software using the Neighbor-Joining method. Selected model strains: Bacillus altitudinis 41KF2b (MZ276300.1), Bacillus pumilus ATCC 7601 (OQ876682.1), Bacillus australimaris MCCC 1A05787 (NR148787.1), Bacillus safensis NBRC 100820 (ON878131.1), Bacillus sonorensis NBRC101234 (KT989851.1), Bacillus haynesii NRRL B-41327 (OQ921694.1), Bacilluslicheniformis ACCT14580 (ON597434.1), Bacillus atrophaeus NBRC 15539(NR112723.1), Bacillus spizizenii NBRC 101239 (NR112686.1), Bacillus stercoris JCM 30051 (NR 180796.1), Bacillus siamensis KCTC13613 (PQ764821.1), Bacillus velezensis BCRC 17467 (EF433407.1), Bacillus firmus JCM 2512 (LC379133.1), Bacillus thuringiensis IAM 12077 (NR043403.1), Enterococcus durans NBRC 100479 (NR113900.1), of which Enterococcus durans NBRC 100479 (NR113900.1) was an outgroup. The results are shown in Figure 9 The phylogenetic tree showed that the strain B08 was most closely related to Bacillus altitudinis 41KF2b (MZ276300.1).

[0032] SEQ ID No. 3, 16S rDNA of B35:

[0033] SEQ ID No. 4, 16S rDNA of B08:

[0034] Example 2 Experiment on promoting rice blast resistance in detached leaves using single microorganisms and their combination After sporulation, the spores of rice blast fungus P131 were eluted with 0.025% (v / v) Tween20 aqueous solution to prepare a suspension, and the concentration was adjusted to 1×10 5 One-month-old rice seedlings of similar growth were selected as test subjects. Detached rice leaves were cut and lightly scratched with a syringe needle for subsequent inoculation. Four treatments, including single and combined bacterial solutions, were set up: B35, B08, and B35 + B08. A spore suspension of rice blast fungus plus sterile water served as the control group.

[0035] Preparation method of each single colony liquid: under sterile conditions, first streak the LB culture plate to activate the endogenous bacteria preserved in glycerol, add 1 mL of liquid LB to a sterile 2 mL test tube, pick the single colony on the activated bacterial plate with a sterile toothpick and place it in the test tube, place it in a 37°C shaker, shake at 180 r / min for 12 h, take 1 mL of the shaken mother liquid and add it to a 100 mL small conical flask containing 50 mL of LB, place it in a 37°C shaker, shake at 180 r / min for 24 h, take the re-shaken bacterial liquid, pour it into a 50 mL centrifuge tube, place it in a centrifuge, centrifuge at 5000 rpm for 10 min, discard the supernatant, resuspend the bacteria with sterile water and dilute it to a bacterial concentration of 10 8 CFU / mL, used as bacterial suspension for future use.

[0036] When studying the function of single microorganism B35 on rice blast resistance, 1 mL of the microorganism was taken at a concentration of 1×10 5 / mL of rice blast fungus spore suspension, and 1mL of 10 8 After evenly mixing a 10 μL suspension of the biocontrol bacterium B35 at a concentration of 500 CFU / mL, inoculate the wound site with 10 μL. For the control group, sterile water was used to replace the suspension volume (CK) to ensure equal and even inoculation. The inoculations were sealed with parafilm and incubated in the dark at 28°C. After 36 hours, the inoculations were exposed to light, ensuring moisture retention. The inoculation results were observed after 7 days. The experiment was repeated three times. The method for single strain B08 was the same as for B35.

[0037] When making binary compound, take 1mL with a concentration of 1×10 5 / mL of rice blast fungus spore suspension, and 0.5mL of 10 8 CFU / mL of B35 and 0.5mL concentration of 10 8 CFU / mL of B08 were mixed evenly, and 10ul was inoculated on the scratched part. The control group used sterile water instead of the bacterial solution volume (CK). Figure 2As shown in the figure, when the mixture of B35, B08 and rice blast fungus spore suspension was inoculated respectively, the lesion area of rice leaves was reduced to a certain extent. When B35 and B08 were used in combination, the lesion area of rice leaves was significantly reduced.

[0038] Example 3 Potted experiment on promoting rice blast resistance using single microorganisms and their combination In the pot spray inoculation test, the spores of rice blast fungus P131 were eluted with 0.025% (v / v) Tween20 aqueous solution to prepare a suspension, and the concentration was adjusted to 1×10 5 Four treatment groups, including single bacterial suspension and compound bacterial suspension, were set up: B35, B08, and B35+B08. A spore suspension of rice blast fungus + sterile water served as the control group (CK).

[0039] When studying the function of single microorganism B35 on rice blast resistance, 10 mL of the microorganism was taken with a concentration of 1×10 5 / mL of rice blast fungus spore suspension, and 10ml of 10 8 CFU / mL of the biocontrol bacteria B35 solution was evenly mixed and set aside; the method for strain B08 was the same as that for B35. When making a binary compound, take 10mL of a concentration of 1×10 5 / mL of rice blast fungus spore suspension, and 5mL of 10 8 The concentration of B35 in 5 mL was 10 CFU / mL. 8 Mix B08 at a concentration of 100 CFU / mL and use them evenly. For the control group, sterile water was used instead of the bacterial suspension volume (CK). One-month-old rice seedlings with similar growth characteristics were selected as test subjects. The spore suspension was evenly sprayed on the rice leaves using a sprayer. The inoculated rice was incubated at 28°C, high humidity, and in the dark for the first 36 hours, followed by full light conditions. Observe and investigate the disease on day 7 (dpi). Figure 3 ); It was found that when different biocontrol bacteria were used to prepare the rice blast fungus composite solution, the disease incidence of rice leaves was reduced to a certain extent after adding single-plant biocontrol bacteria. When B35 and B08 were used in combination, the area of rice leaf spots was significantly reduced.

[0040] Example 4 Detection of Growth-Promoting Performance of Single Microorganisms In order to clarify whether the isolated rice leaf microorganism B35 is related to rice growth promotion, B35 was tested for phosphorus solubility, potassium solubility, nitrogen fixation, IAA production, and siderophore production.

[0041] Determination of nitrogen fixation ability of strains: The purified strains were inoculated into LB liquid medium at 30°C and 200 r·min. -1After 24 hours of shaking culture, 5 μL of the bacterial solution was inoculated into the center of the Axubei nitrogen-fixing medium. Repeat three times for each strain. Incubate at 30°C upside down for 3 days and observe the growth of the strain. If the strain grows normally, it is considered to have the ability to fix nitrogen.

[0042] Determination of the phosphate-solubilizing ability of a strain: Inoculate the purified strain in the center of an organic or inorganic phosphate medium and incubate inverted at 30°C for 5 days. If a clear zone forms around the colony, the strain is considered phosphate-solubilizing. Measure the diameter of the clear zone (D) and the colony diameter (d). Calculate the ratio of the clear zone diameter to the colony diameter to determine the strain's phosphate-solubilizing ability.

[0043] Determination of potassium-solubilizing ability of strains: Inoculate the purified strain in the center of Alexandrov's culture medium and incubate at 30°C for 5 days. If a clear zone forms around the colony, it is considered to have potassium-solubilizing ability. Measure the diameter of the clear zone (D) and the colony diameter (d), and calculate the ratio of the clear zone diameter to the colony diameter to determine the potassium-solubilizing ability of the strain.

[0044] Determination of the siderophore production capacity of the strain: Inoculate the purified strain in the center of CAS assay medium and incubate inverted at 30°C for 3–5 days. If an orange halo forms around the colony, the strain is considered to have the ability to produce siderophores. Measure the orange siderophore halo (D) and colony diameter (d), and calculate their ratio.

[0045] The ability of strains to secrete indoleacetic acid (IAA) was determined by taking 10 μL of bacterial solution (OD 600 =1.0) was inoculated into 20 mL of a flask containing L-tryptophan (100 mg·L -1 ) in LB liquid medium, 30 ℃, 200 r·min -1 After 3 days of shaking culture, the bacterial suspension was taken at 8000 r·min -1 Centrifuge for 10 minutes. Take 0.5 mL of the supernatant and add an equal volume of Salkowski colorimetric solution. Use blank culture medium as a control. Incubate at room temperature in the dark for 30 minutes and observe the color change. If the solution turns red, IAA is produced.

[0046] like Figure 4 、 Figure 5 As shown in Table 2, Pseudomonas orvzihabitans (B35) has good abilities of solubilizing phosphate, solubilizing potassium, fixing nitrogen, producing IAA and producing siderophores.

[0047] Table 2 Growth-promoting performance test Example 5 Experiment on promoting rice seed germination by a single microorganism Soak rice seeds in 70% alcohol for 1 minute, then in 1% sodium hypochlorite solution for 10 minutes, and finally rinse with sterile water 5 times for surface disinfection. Under sterile conditions, first streak the LB culture plate to activate the endophytic bacteria stored in glycerol. Add 1 mL of liquid LB to a sterile 2 mL test tube. Use a sterile toothpick to pick a single colony on the activated bacterial plate and place it in the test tube. Place it in a 37°C shaker at 180 r / min for 12 hours. Then, take 1 mL of the shaken mother liquid and add it to a 100 mL small conical flask containing 50 mL of LB. Place it in a 37°C shaker at 180 r / min for 24 hours. Take the re-shaken bacterial liquid and pour it into a 50 mL centrifuge tube. Place it in a centrifuge and centrifuge at 5000 rpm for 10 minutes. Discard the supernatant and resuspend the bacteria in sterile water and dilute it to a bacterial concentration of 10 8 CFU / mL was used as a bacterial suspension for future use. Seeds were soaked in different bacterial solutions. After 24 hours, the treated seeds were placed in a Petri dish lined with filter paper and an appropriate amount of sterile water was added to moisten the filter paper. Culture was performed at 28°C under 14 hours of light / 10 hours of darkness. After 7 days, seed germination was analyzed, including germination rate, shoot length, and root length.

[0048] The results are as follows Figure 6 and as shown in Table 3. The results showed that the germination rate and root length of rice seeds were significantly improved after B35 treatment, and there were significant differences compared with CK (sterile water), indicating that B35 treatment can promote the germination of rice seeds.

[0049] Table 3 Effects of growth-promoting strains on rice seed germination rate, root length and sprout length Example 6 Experiment on promoting rice growth by a single microorganism Select a matrix mixed with vermiculite, perlite, peat soil, etc. in a certain proportion and put it into the pot. Soak the rice seeds in 70% alcohol for 1 minute, then soak in 1% sodium hypochlorite solution for 10 minutes, and finally rinse with sterile water 5 times before soaking. Under sterile conditions, first streak the LB culture dish to activate the endophytic bacteria preserved in glycerol, add 1 mL of liquid LB to a sterilized 2 mL test tube, pick a single colony on the activated bacterial plate with a sterilized toothpick and put it in the test tube, place it in a 37°C shaker, shake at 180 r / min for 12 hours, take 1 mL of the shaking mother liquid and add it to a 100 mL small conical flask containing 50 mL of LB, place it in a 37°C shaker, shake at 180 r / min for 24 hours, take the re-shaken bacterial solution, pour it into a 50 mL centrifuge tube, put it into a centrifuge, centrifuge at 5000 rpm for 10 minutes, discard the supernatant, resuspend the bacteria with sterile water and dilute it to a bacterial concentration of 10 8CFU / mL, used as a bacterial suspension. Soak seeds in different bacterial solutions for 24 hours. Sow the treated seeds in the prepared substrate, sowing four seeds per pot with three replicates. Place the pots in a greenhouse at a controlled temperature of 25-30°C and a relative humidity of 60-70%. Water daily to keep the substrate moist. After 30 days, assess the rice's plant height, stem base width, and root length.

[0050] The results are as follows Figure 7 The results are shown in Table 4. The results showed that the root length and stem base width of rice were significantly increased after B35 treatment, which was significantly different from CK (sterile water), indicating that B35 treatment can promote rice growth.

[0051] Table 4 Effects of growth-promoting strains on plant height, root length and stem base width of rice seedlings The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A strain of Pseudomonas orvzihabitans B35, deposited with GDMCC No: 66230.

2. Use of the Pseudomonas oryzae according to claim 1 in the preparation of phosphate-dissolving, potassium-dissolving and / or nitrogen-fixing products.

3. Use of the Pseudomonas oryzae according to claim 1 in producing IAA and / or siderophore.

4. Use of the Pseudomonas oryzae according to claim 1 in resisting rice blast.

5. A product for dissolving phosphorus, potassium and / or fixing nitrogen, characterized in that: The main components thereof are the Pseudomonas oryzae according to claim 1, the fermentation product of the Pseudomonas oryzae according to claim 1 or / and the secretion of the Pseudomonas oryzae according to claim 1.

6. A product containing IAA and / or siderophore, characterized in that: The main components thereof are the Pseudomonas oryzae according to claim 1, the fermentation product of the Pseudomonas oryzae according to claim 1 or / and the secretion of the Pseudomonas oryzae according to claim 1.

7. A preparation for preventing and controlling rice blast, characterized in that: Containing the Pseudomonas oryzae according to claim 1, a fermentation product of the Pseudomonas oryzae according to claim 1 or / and a secretion of the Pseudomonas oryzae according to claim 1.

8. The preparation according to claim 7, characterized in that: The preparation also contains Bacillus saltitudinis B08, whose deposit number is GDMCC No: 66286.

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