Fingerprint spectrum detection method of Xianqing decoction, fingerprint spectrum of Xianqing decoction and application of fingerprint spectrum

Abstract

The invention belongs to the technical field of quality control of traditional Chinese medicines, and particularly relates to a fingerprint spectrum detection method of Xianqing decoction as well as a fingerprint spectrum and application of the fingerprint spectrum detection method. The fingerprint spectrum detection method of the Xianqing decoction comprises the following steps: preparing a test solution; preparing a mixed reference substance solution; sampling the test solution and the mixed reference solution, and detecting by adopting a high performance liquid chromatography; a standard fingerprint spectrum is established by adopting a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system (2012 edition). The fingerprint spectrum detection method of the Xianqing decoction has the characteristics of short analysis time and high separation efficiency, can simultaneously monitor a plurality of characteristic components of a plurality of medicinal materials in a formula, realizes overall quality control of bitter apricot kernel, licorice root, ginger, rhizoma atractylodis and other medicinal materials in the Xianqing decoction in a classic famous formula, and has a wide application prospect. And a reliable technical means is provided for quality standardization research of classic famous prescriptions.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine quality control, and particularly relates to a fingerprint spectrum detection method for Xianshu decoction, a fingerprint spectrum thereof, and an application thereof. Background Art

[0002] Xianshu Tang, originating from Volume 10 of the Song Dynasty's "Taiping Huimin Hejiju Fang," consists primarily of braised Atractylodes lancea, jujube, honey-roasted licorice, braised almonds, and roasted dried ginger. It boasts multiple benefits, including warding off plague, dispelling cold and dampness, warming the spleen and stomach, and promoting appetite. Atractylodes lancea serves as the main ingredient, clearing dampness and strengthening the spleen, dispelling wind and cold, and improving eyesight. Jujube serves as the auxiliary ingredient, tonifying the middle and replenishing qi, nourishing blood and calming the mind, enhancing its spleen and stomach tonic effects. Licorice serves as the adjuvant, tonifying the spleen and replenishing qi, dispelling phlegm and relieving coughs, and harmonizing the various ingredients. Roasted licorice, with its sweet flavor, enters the spleen and replenishes spleen and stomach qi, making the formula more gentle without damaging vital energy. Dried ginger warms the middle and dispels cold, restores yang and unblocks the meridians, warms the lungs and eliminates dampness. Bitter almonds descend qi, relieve coughs and asthma, and their bitterness helps lower stomach qi. As a seed-based plant, they exert a laxative effect, moistening the intestines and promoting bowel movements.

[0003] While experts and scholars have conducted extensive research on the individual herbs in Xianshu Tang, little research has been conducted on its overall composition and efficacy. Furthermore, methods for fingerprint detection of Xianshu Tang are still lacking. Fingerprinting is one of the most important tools for quality control of traditional Chinese medicine compound preparations. It provides a comprehensive qualitative evaluation of the compound's flavor profile, reflecting the underlying substance of its efficacy and verifying its authenticity. Therefore, a method for fingerprint detection of Xianshu Tang is urgently needed. Summary of the Invention

[0004] To establish a method for detecting Xianshu Decoction fingerprints, the present invention aims to provide a method for establishing the HPLC fingerprint of Xianshu Decoction, thereby ensuring and supporting comprehensive and effective quality control of Xianshu Decoction. The present invention provides a method for detecting Xianshu Decoction fingerprints, a fingerprint, and applications thereof. To achieve the above objectives, the present invention employs the following technical solutions.

[0005] The first object of the present invention is to provide a fingerprint detection method for Xianshu Decoction, comprising the following steps: Preparation of the test solution: Take the corresponding Chinese medicinal material raw materials of Xianshu Decoction, add methanol to extract, and filter to obtain the test solution. Among them, the Chinese medicinal materials include Atractylodes lancea, jujube, licorice, bitter almonds and dried ginger powder. The present invention selects the above raw materials to prepare the test solution. The dosage and ratio used are all based on the "Taiping Huimin Hejijufang". Based on the 5g compound amount, the dosage of each medicinal material is 2.61g Atractylodes lancea, 1.232g jujube, 0.76g licorice, 0.328g bitter almonds and 0.08g dried ginger.

[0006] Preparation of mixed reference solution: Take amygdalin reference substance, rutin reference substance, liquiritin reference substance, 6-gingerol reference substance and atractylodesin reference substance, add methanol to dissolve, filter, and obtain a mixed reference solution.

[0007] The test solution and the mixed reference solution were sampled respectively, and fingerprint detection was performed using high performance liquid chromatography, and the chromatograms were recorded to obtain the test sample fingerprint and the reference chromatogram, respectively. The "Chinese Herbal Chromatographic Fingerprint Similarity Evaluation System" (2012 Edition) was used to establish a standard fingerprint, and the fingerprint of Xianshu Decoction was formulated. Wherein, the chromatographic conditions of the high performance liquid chromatography are as follows: The chromatographic column used was an octadecylsilane bonded silica gel column; the detection wavelength was 206 nm to 208 nm; the injection volume was 4.8 μL to 5.2 μL; acetonitrile was used as mobile phase A, and 0.1% by volume phosphoric acid solution was used as mobile phase B. Gradient elution was performed according to the following table: .

[0008] It should be noted that as the retention time increases, the volume of mobile phase A gradually increases, while the volume of mobile phase B gradually decreases. From 22 to 32 minutes, the volume of mobile phase A gradually increases from 33% to 55%, while the volume of mobile phase B gradually decreases from 67% to 45%. From 32 to 36 minutes, the volume of mobile phase A stabilizes at 55%, while the volume of mobile phase B stabilizes at 45%. From 36 to 44 minutes, the volume of mobile phase A gradually increases from 55% to 64%, while the volume of mobile phase B gradually decreases from 45% to 36%.

[0009] There are 16 common peaks in the fingerprint of the Xianshu Decoction, and the retention times of the peaks are 13.69min, 16.82min, 17.42min, 22.67min, 37.48min, 40.01min, 40.89min, 43.94min, 45.32min, 46.76min, 50.22min, 50.88min, 52.29min, 53.91min, 54.62min and 55.75min, respectively, of which 13.69min is amygdalin, 16.82min is rutin, 17.42min is liquiritin, 37.48min is 6-gingerol, and 55.75min is atractylodesin.

[0010] The present invention aims to establish a method for detecting the fingerprint of Xianshu Decoction. The present invention aims to provide a method for establishing the HPLC fingerprint of Xianshu Decoction, thereby providing assurance and support for comprehensive and effective quality control of Xianshu Decoction. The present invention provides a method for detecting the fingerprint of Xianshu Decoction, which has the characteristics of short analysis time and high separation efficiency. It can simultaneously monitor multiple characteristic components of multiple medicinal materials in the prescription, achieving overall quality control of the bitter almond, licorice, ginger, and atractylodes in the classic prescription Xianshu Decoction. It has a holistic and characteristic qualitative identification function, and can fill the gap in related methods for detecting the fingerprint of Xianshu Decoction.

[0011] Preferably, the chromatographic column is an AQ-C18 chromatographic column with a specification of 4.6×250 mm and 5 μm.

[0012] Preferably, the chromatographic conditions further include: a flow rate of 0.78 mL / min to 0.82 mL / min.

[0013] Preferably, the chromatographic conditions further include: column temperature: 38°C~42°C.

[0014] Preferably, the mixed reference solution is a mixed solution containing 20 μg / mL of atractylodesin, 40 μg / mL of rutin, 20 μg / mL of liquiritin, 200 μg / mL of amygdalin and 100 μg / mL of 6-gingerol.

[0015] Preferably, during the preparation of the test solution, the conditions for adding methanol for extraction are ultrasonic extraction for 58 min to 62 min.

[0016] The second object of the present invention is to provide the fingerprint of Xianshu Soup measured by the fingerprint detection method of Xianshu Soup.

[0017] Preferably, there are 16 common peaks in the fingerprint of the Xianshu Decoction, and the retention times of the peaks are 13.69min, 16.82min, 17.42min, 22.67min, 37.48min, 40.01min, 40.89min, 43.94min, 45.32min, 46.76min, 50.22min, 50.88min, 52.29min, 53.91min, 54.62min and 55.75min, respectively, of which 13.69min is amygdalin, 16.82min is rutin, 17.42min is liquiritin, 37.48min is 6-gingerol, and 55.75min is atractylodesin.

[0018] The third object of the present invention is to provide the application of the fingerprint detection method of Xianshu Soup in the quality control of Xianshu Soup.

[0019] Preferably, the fingerprint detection method of Xianshu Soup is applied to the quality control of Xianshu Soup, with the fingerprint similarity being greater than 0.9 as the inspection quality control standard.

[0020] Among them, the similarity is calculated according to the "Similarity Evaluation System of Chinese Medicine Chromatographic Fingerprints" (2012 edition).

[0021] The "similarity" in the TCM chromatographic fingerprint similarity evaluation system is a quantitative indicator. The closer the value is to 1, the more similar the chemical composition characteristics of the two are. It is used to evaluate the degree of similarity between the overall chemical feature profiles of the chromatographic fingerprint of the tested TCM sample and the reference fingerprint.

[0022] Compared with the prior art, the present invention has the following beneficial effects: 1. In order to establish a method for fingerprint detection of Xianshu Decoction, the present invention provides a fingerprint detection method for Xianshu Decoction, which comprises the following steps: preparing a test solution, preparing a mixed reference solution, sampling the test solution and the mixed reference solution, and performing fingerprint detection by high performance liquid chromatography. The fingerprint detection method for Xianshu Decoction provided by the present invention has the characteristics of short analysis time and high separation efficiency, can simultaneously monitor multiple characteristic components of multiple medicinal materials in the prescription, and realizes the overall quality control of bitter almonds, licorice, ginger, atractylodes, and other medicinal flavors in the classic Xianshu Decoction. It has an overall and characteristic qualitative identification function, and can fill the problem of the vacancy of related methods for fingerprint detection of Xianshu Decoction.

[0023] 2. The fingerprint detection method for Xianshu Decoction provided by the present invention has the characteristics of short analysis time and high separation efficiency. It can simultaneously monitor multiple characteristic components of multiple medicinal materials in the prescription, and realize the overall quality control of bitter almonds, licorice, ginger, atractylodes and other medicinal ingredients in the classic prescription Xianshu Decoction, providing a reliable technical means for the quality standardization research of classic prescriptions. 3. The fingerprint spectrum detection method of the Xianshu Soup provided by the present invention also has the advantages of high precision, good repeatability, good stability, and simple sample processing, and can be used for the quality control of Xianshu Soup. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is an overlay of HPLC fingerprints of 18 batches of Xianshu Decoction ethanol extract samples from different origins in the present invention.

[0025] Figure 2 This is the characteristic spectrum of 18 batches of Xianshu Decoction ethanol extract samples from different origins in the present invention.

[0026] Figure 3 This is a diagram of the mixed standard in the present invention; wherein, 1 is amygdalin; 2 is rutin; 3 is liquiritin; 4 is gingerol; and 5 is atractylodesin. DETAILED DESCRIPTION

[0027] The present invention will be described in detail below with reference to the accompanying drawings and specific examples, but they should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0028] The instruments, reagents and medicinal materials used in the following examples are as follows: (1) Instruments 1260 high performance liquid chromatograph (Agilent, USA), BP211D 1 / 100,000 electronic analytical balance (Sartorius, Germany).

[0029] (2) Reagents Reference substances: atractylodesin reference substance (purity ≥98%, batch number PS013486), rutin reference substance (purity ≥98%, batch number AD1520), liquiritin reference substance (purity ≥98%, batch number AB0619), amygdalin (reference substance purity ≥98%, batch number AB1129), 6-gingerol reference substance (purity ≥98%, batch number ABL1253).

[0030] Atractylodesin was purchased from Shanghai Yuanye Biotechnology Co., Ltd., and other reference substances were purchased from Chengdu Alfa Biotechnology Co., Ltd.

[0031] Methanol, acetonitrile, and phosphoric acid were all of chromatographic grade.

[0032] (3) Preparation of medicinal pieces The preparation and processing methods of each medicinal material refer to the 2020 edition of the Chinese Pharmacopoeia Volume 1 and the Taiping Huimin Hejijufang. The specific methods are as follows: Atractylodes: Remove impurities, wash, soak, cut into thick slices, dry, heat the frying container until smoke comes out when bran is sprinkled in, then add the Atractylodes, stir quickly, and fry until the surface turns yellow or dark yellow, take out, sieve out the bran, let cool, grind into powder, and set aside.

[0033] Jujube: Remove impurities, wash, and dry in the sun. Crack or remove the core before use, grind into powder, and set aside.

[0034] Licorice: Remove impurities from the licorice slices, wash, soak thoroughly, cut into thick slices, and dry. To prepare the honey roast, dilute the refined honey with an appropriate amount of boiling water, add the honey to the licorice, mix thoroughly, and let it simmer thoroughly. Place the licorice in a stir-fry container and stir-fry over low heat until the desired consistency is achieved. Remove the licorice slices, let them cool, and grind into a powder for later use.

[0035] Bitter almonds: Heat the frying container until smoke starts to rise as soon as bran is sprinkled in. Then add the bitter almonds and stir quickly. Fry until the surface turns yellow or dark yellow. Remove from heat, sieve out the bran, let cool, grind into powder, and set aside.

[0036] Dried ginger: Remove impurities, soak briefly, wash, moisten thoroughly, cut into thick slices or blocks, and dry. Place clean river sand in a stir-fry container and heat over high heat until smooth. Add the dried ginger and stir-fry constantly until the surface is puffy and crispy, or until it reaches the desired consistency. Remove, sieve out the river sand, let cool, and grind into a powder for later use.

[0037] Example 1: Establishment and application of the fingerprint detection method of Xianshu Decoction 1. Solution preparation (1) Preparation of test solution Accurately weigh 2.61 g of Atractylodes lancea, 1.232 g of jujube, 0.76 g of liquorice, 0.328 g of bitter almond and 0.08 g of dried ginger powder and place them together in the same stoppered conical flask, add 13 mL of methanol, weigh the weight, and extract by ultrasonic for 60 min. Take out, cool to room temperature, make up the lost mass with methanol, shake well, filter with 0.22 μm microporous membrane, and take the filtrate to obtain the fingerprint test solution.

[0038] Among them, Atractylodes lancea, jujube, licorice, bitter almonds and dried ginger powder are the raw materials of Chinese medicinal materials.

[0039] (2) Preparation of mixed reference solution Accurately weigh appropriate amounts of atractylodesin reference substance, rutin reference substance, liquiritin reference substance, amygdalin reference substance, and 6-gingerol into a 10 mL volumetric flask, and add methanol to prepare a mixed reference solution containing 20 μg / mL of atractylodesin, 40 μg / mL of rutin, 20 μg / mL of liquiritin, 200 μg / mL of amygdalin, and 100 μg / mL of 6-gingerol.

[0040] Among them, the mixed reference solution is also called the mixed standard solution.

[0041] 2. Chromatographic conditions (1) Chromatographic column: Octadecylsilane bonded silica gel as filler, AQ-C18 chromatographic column, 4.6×250mm, 5μm.

[0042] (2) Flow rate: 0.8 mL / min.

[0043] (3) Column temperature: 40°C.

[0044] (4) Detection wavelength: 207nm.

[0045] (5) Injection volume: 5 μL.

[0046] (6) Mobile phase: Acetonitrile was used as mobile phase A and 0.1% by volume formic acid solution was used as mobile phase B for gradient elution. The gradient elution conditions are shown in Table 1. The percentages in the mobile phase are by volume.

[0047] Table 1 Elution conditions It should be noted that as the retention time increases, the volume of mobile phase A gradually increases, while the volume of mobile phase B gradually decreases. From 22 to 32 minutes, the volume of mobile phase A gradually increases from 33% to 55%, while the volume of mobile phase B gradually decreases from 67% to 45%. From 32 to 36 minutes, the volume of mobile phase A stabilizes at 55%, while the volume of mobile phase B stabilizes at 45%. From 36 to 44 minutes, the volume of mobile phase A gradually increases from 55% to 64%, while the volume of mobile phase B gradually decreases from 45% to 36%.

[0048] 3. Establishment of HPLC fingerprint Take samples from each batch of Xianshu Soup and treat them according to the method for preparing the test solution in step 1 above to obtain the test solution of each batch of Xianshu Soup. Detailed information on each batch of Xianshu Soup samples is shown in Table 2.

[0049] Table 2 Detailed information of each batch of Xianshu Decoction samples The test solution was injected into the high performance liquid chromatograph and measured according to the chromatographic conditions in step 2, and the HPLC chromatogram was recorded. The measured spectrum was imported into the "Chinese Herbal Chromatographic Fingerprint Similarity Evaluation System 2012 Edition" for chromatographic peak matching analysis. The chromatogram of sample No. 1 was used as the reference spectrum, the time window was set to 0.1, and multi-point calibration automatic matching and Mark peak matching were used to generate a common peak pattern. The median method was used to generate a reference spectrum. The superposition diagram and characteristic spectrum of the HPLC fingerprint spectrum of 18 batches of Xianshu Decoction were established, as shown in the figure. Figure 1 and Figure 2 shown.

[0050] Inject the mixed standard solution into the high performance liquid chromatograph, measure according to the chromatographic conditions in step 2, and record the HPLC chromatogram, such as Figure 3 shown.

[0051] Depend on Figure 1 and Figure 2 It can be seen that the composition of Xianshutang samples between different batches is generally similar, with small stability and fluctuation range.

[0052] Depend on Figure 3 The peak positions of amygdalin, rutin, liquiritin, 6-gingerol, and atractylodesin can be seen.

[0053] 4. Establishment of fingerprint spectrum and similarity evaluation Prepare the test sample solutions from each batch of Xianshu Decoction and inject them according to the chromatographic conditions in step 2 above. Record the chromatograms. Analyze the HPLC chromatograms of each batch of Xianshu Decoction using the Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2012 Edition).

[0054] The spectrum of the S1 sample was used as a reference spectrum, the time window was 0.1 min, the median number method was used, and peak matching was performed after multi-point calibration to generate a reference fingerprint spectrum (R). The results are shown in Figure 1 , calculate the similarity, and the results are shown in Table 3.

[0055] Table 3 Similarity results of fingerprint spectra of 18 batches of Xianshutang samples As shown in Table 3, the similarities of the fingerprints of the 18 batches of Xianshutang samples ranged from 0.900 to 0.998, all greater than 0.90. The results showed that the quality of the 18 batches of Xianshutang samples was basically consistent.

[0056] Example 2: Methodological Investigation 1. Precision experiment: Prepare one portion of the test solution (S1), and inject it continuously six times according to the chromatographic conditions in Example 1 above. Record the chromatogram, and calculate the similarity according to the "Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine" (2012 edition). At the same time, calculate the RSD values of the relative retention time and relative peak area of each common peak.

[0057] The similarity results are shown in Table 4.

[0058] Table 4 Fingerprint instrument precision results As shown in Table 4, the similarities between the fingerprint of the test solution (S1) and the control fingerprint were greater than 0.99, and the RSD values of the relative peak areas of amygdalin, rutin, liquiritin, 6-gingerol and atractylodesin were 1.82, 1.5, 1.45, 1.24 and 1.21, respectively, indicating that the instrument had good precision.

[0059] 2. Stability test: Prepare one portion of the test solution (S1) and measure the solution according to the chromatographic conditions in Example 1 at 0 h, 2 h, 4 h, 8 h, 12 h, and 24 h, respectively. Calculate the similarity according to the "Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine" (2012 edition). At the same time, calculate the RSD values of the relative retention time and relative peak area of each common peak.

[0060] The similarity results are shown in Table 5.

[0061] Table 5 Fingerprint instrument stability results As shown in Table 5, the similarities between the fingerprint of the test solution (S1) and the control fingerprint were greater than 0.98, and the RSD values of the relative peak areas of amygdalin, rutin, liquiritin, 6-gingerol and atractylodesin were 1.8, 1.67, 1.93, 3.94 and 1.64, respectively, indicating that the test solution (S1) had good stability within 24 h.

[0062] 3. Repeatability experiment: Take S1 sample to prepare 6 test sample solutions in parallel, measure according to the chromatographic conditions in Example 1 above, record the chromatogram, calculate the similarity according to the "Similarity Evaluation System of Chinese Medicine Chromatographic Fingerprint" (2012 edition), and calculate the RSD values of the relative retention time and relative peak area of each common peak.

[0063] The similarity results are shown in Table 6.

[0064] Table 6 Fingerprint instrument repeatability results As shown in Table 6, the similarities between the fingerprints of the test sample and the control were greater than 0.99, and the RSD values of the relative peak areas of amygdalin, rutin, liquiritin, 6-gingerol and atractylodesin were 2.6, 3.31, 3.32, 2.94 and 2.08, respectively, indicating that the method had good repeatability.

[0065] From the above experimental results, it can be seen that the fingerprint detection method of Xianshu Decoction has the advantages of high precision, good repeatability, good stability, and simple sample processing, and can be used for the quality control of Xianshu Decoction.

[0066] It should be noted that when the present invention involves a numerical range, it should be understood that both endpoints of each numerical range and any value between the two endpoints can be selected. To avoid redundancy, the present invention describes a preferred embodiment.

[0067] Although preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once the basic inventive concepts become known, and all such changes and modifications fall within the scope of the present invention.

Claims

1. A fingerprint detection method for Xianshu Decoction, characterized in that: The steps include: Preparation of test solution: Take the corresponding Chinese medicinal material of Xianshu Decoction, add methanol to extract, filter, and obtain the test solution; Preparation of mixed reference solution: dissolve amygdalin reference substance, rutin reference substance, liquiritin reference substance, 6-gingerol reference substance and atractylodesin reference substance in methanol, filter and obtain mixed reference solution; The test solution and the mixed reference solution are sampled respectively, and fingerprint detection is performed using high performance liquid chromatography, and chromatograms are recorded to obtain fingerprints of the test sample and chromatograms of the reference solution, respectively, and formulate the fingerprint of Xianshu Decoction; Wherein, the chromatographic conditions of the high performance liquid chromatography are as follows: The chromatographic column used was an octadecylsilane bonded silica gel column; the detection wavelength was 206 nm to 208 nm; the injection volume was 4.8 μL to 5.2 μL; acetonitrile was used as mobile phase A, and 0.1% by volume phosphoric acid solution was used as mobile phase B. Gradient elution was performed according to the following table: ； There are 16 common peaks in the fingerprint of the Xianshu Decoction, and the retention times of the peaks are 13.69min, 16.82min, 17.42min, 22.67min, 37.48min, 40.01min, 40.89min, 43.94min, 45.32min, 46.76min, 50.22min, 50.88min, 52.29min, 53.91min, 54.62min and 55.75min, respectively, of which 13.69min is amygdalin, 16.82min is rutin, 17.42min is liquiritin, 37.48min is 6-gingerol, and 55.75min is atractylodesin.

2. The fingerprint detection method of Xianshu Decoction according to claim 1, characterized in that: The chromatographic column is an AQ-C18 chromatographic column with a specification of 4.6×250 mm and 5 μm.

3. The fingerprint detection method of Xianshu Decoction according to claim 1, characterized in that: The chromatographic conditions also include: a flow rate of 0.78 mL / min to 0.82 mL / min.

4. The fingerprint detection method of Xianshu Decoction according to claim 1, characterized in that: The chromatographic conditions also include: column temperature: 38°C~42°C.

5. The fingerprint detection method of Xianshu Decoction according to claim 1, characterized in that: The mixed reference solution is a mixed solution containing 20 μg / mL of atractylodesin, 40 μg / mL of rutin, 20 μg / mL of liquiritin, 200 μg / mL of amygdalin, and 100 μg / mL of 6-gingerol.

6. The fingerprint detection method of Xianshu Decoction according to claim 1, characterized in that: During the preparation of the test solution, the conditions for adding methanol for extraction are ultrasonic extraction for 58 min to 62 min.

7. The fingerprint of Xianshu Soup measured by the fingerprint detection method of Xianshu Soup according to claim 1.

8. The fingerprint of the Xianshu Decoction according to claim 7, characterized in that: There are 16 common peaks in the fingerprint of the Xianshu Decoction, and the retention times of the peaks are 13.69min, 16.82min, 17.42min, 22.67min, 37.48min, 40.01min, 40.89min, 43.94min, 45.32min, 46.76min, 50.22min, 50.88min, 52.29min, 53.91min, 54.62min and 55.75min, respectively, of which 13.69min is amygdalin, 16.82min is rutin, 17.42min is liquiritin, 37.48min is 6-gingerol, and 55.75min is atractylodesin.

9. Application of the fingerprint detection method of Xianshu Soup according to claim 1 in the quality control of Xianshu Soup.

10. The use according to claim 9, characterized in that The fingerprint detection method of Xianshu Decoction was applied to the quality control of Xianshu Decoction, and the fingerprint similarity greater than 0.9 was used as the inspection quality control standard.

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