Seedling raising method for improving germination rate of zelkova schneideriana seeds

Abstract

The invention discloses a seedling raising method for improving the germination rate of zelkova schneideriana seeds, and belongs to the field of agricultural planting, the seedling raising method comprises the following steps: S1, seed screening: selecting full zelkova schneideriana seeds without plant diseases and insect pests, rinsing, and removing empty shell seeds floating on the water surface; s2, seed disinfection: disinfecting the seeds with a 10% NaClO solution for 10 min, and continuously shaking the seeds during disinfection; after disinfection, washing the seeds with sterile water for 7-8 times until no NaClO residue is left on the epidermis of the seeds; s3, hormone seed soaking treatment, wherein the disinfected zelkova schneideriana seeds are put into a hormone solution to be soaked for 1 h; s4, cleaning the seeds with sterile water for 5-6 times; s5, seedling raising. According to the method, actual operation steps of zelkova schneideriana seedling raising and hormone types and concentrations suitable for zelkova schneideriana seed development are given, so that the germination rate of zelkova schneideriana seeds in the actual production process is increased. The influence of the hormones with different concentrations on the development of the zelkova schneideriana seeds is given, so that the requirements on zelkova schneideriana seedling culture in actual production are met.

Description

Technical Field

[0001] The invention belongs to the field of agricultural planting, and in particular relates to a seedling raising method for improving the germination rate of big-leaf beech seeds. Background Art

[0002] The large-leafed zelkova, also known as the chicken oil tree and yellow gardenia elm, is an evergreen deciduous tree of the genus Zelkova in the family Ulmaceae. Its wood is dense and hard, corrosion-resistant, and has a beautiful grain. Because old wood often exhibits a reddish color, it is known as "blood beech," making it a high-quality material for the manufacture of ships, bridges, vehicles, furniture, and equipment.

[0003] Due to its high economic value, large-scale logging over the past few decades has led to a gradual decline in its population and distribution. It has been listed on China's National Class II Protected Plant List and the IUCN Red List of Threatened Species. Therefore, increasing its population has become a major research focus for domestic researchers. Because its seeds often have empty shells and a low germination rate, increasing their germination rate and ensuring the healthy growth of seedlings are crucial for increasing their numbers. Summary of the Invention

[0004] The purpose of the present invention is to solve the above technical problems and to improve the germination rate of beech seeds.

[0005] To achieve the above objectives, the specific plan is as follows:

[0006] A seedling raising method for improving the germination rate of big-leaf beech seeds comprises the following steps:

[0007] S1: Seed screening: Select plump, disease-free and insect-free big-leaf beech seeds and rinse them with water to remove empty shelled seeds floating on the water surface;

[0008] S2: Seed disinfection: disinfect with 10% NaClO solution for 10 minutes, shaking continuously; after disinfection, rinse with sterile water 7-8 times until there is no NaClO residue on the seed surface;

[0009] S3: Hormone soaking treatment: the sterilized big-leaf beech seeds were placed in the hormone solution and soaked for 1 hour;

[0010] S4: Wash the seeds 5-6 times with sterile water;

[0011] S5: Place the hormone-treated seeds in a germination box with a size of 10 cm × 10 cm and lined with cotton wool soaked in sterile water; then place the cotton wool germination box in a constant temperature and light box at 24°C, and water it every day to ensure that the cotton wool is moist.

[0012] Further, the hormone solution includes BR, GA, 6-6-BA, or IAA.

[0013] Furthermore, the concentration of BR solution is 8 mg·L -1 ~25mg·L -1 .

[0014] Furthermore, the concentration of GA solution was 120 mg·L -1 ~500mg·L -1 .

[0015] Furthermore, the concentration of 6-BA solution was 18 mg·L -1 ~85mg·L -1 .

[0016] Furthermore, the concentration of the IAA solution was 95 mg·L -1 ~305mg·L -1 .

[0017] In summary, the present invention has the following beneficial effects compared to the prior art:

[0018] This paper presents the practical steps for growing beech seedlings and the types and concentrations of hormones suitable for beech seed development, aiming to improve the germination rate of beech seeds during actual production. The paper also describes the effects of different hormone concentrations on beech seed development, addressing the practical needs of beech seedling cultivation in actual production. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0020] Figure 1 The present invention is a flow chart of a seedling raising method for improving the germination rate of big-leaf beech seeds;

[0021] Figure 2 This is a comparison chart of germination rates between water and 6-BA20, 6-BA40, and 6-BA80 treatments;

[0022] Figure 3 This is a comparison chart of germination rates between water and BR10, BR20, and BR30 treatments;

[0023] Figure 4 This is a comparison chart of germination rates between water and GA125, GA250, and GA500 treatments;

[0024] Figure 5 This is a comparison chart of germination rates between water and IAA100, IAA200, and IAA300 treatments;

[0025] Figure 6This is a comparison chart of the germination rates of water and ABA50, ABA100, and ABA150 treatments. DETAILED DESCRIPTION

[0026] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments of the present invention can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0027] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form can also include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0028] Unless otherwise specifically stated, the relative arrangement of the parts and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to actual proportional relationships. The techniques, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the techniques, methods and equipment should be considered as part of the authorized specification. In all examples shown and discussed herein, any specific values should be interpreted as being merely exemplary and not as limitations. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it does not need to be further discussed in subsequent figures.

[0029] See also Figure 1 As shown, the present invention provides a seedling raising method for improving the germination rate of big-leaf beech seeds, comprising the steps of:

[0030] S1: Seed screening: Select plump, disease-free and insect-free big-leaf beech seeds and rinse them with water to remove empty shelled seeds floating on the water surface;

[0031] S2: Seed disinfection: disinfect with 10% NaClO solution for 10 minutes, shaking continuously; after disinfection, rinse with sterile water 7-8 times until there is no NaClO residue on the seed surface;

[0032] S3: Hormone soaking treatment: the sterilized big-leaf beech seeds were placed in the hormone solution and soaked for 1 hour;

[0033] S4: Wash the seeds 5-6 times with sterile water;

[0034] S5: Place the hormone-treated seeds in a germination box with a size of 10 cm × 10 cm and lined with cotton wool soaked in sterile water; then place the cotton wool germination box in a constant temperature and light box at 24°C, and water it every day to ensure that the cotton wool is moist.

[0035] As a preference, the hormone solution includes brassinolide (BR) solution, gibberellin (GA), 6-benzyladenine (6-BA), or indoleacetic acid (IAA).

[0036] As a preferred embodiment, the concentration of BR solution is 8 mg·L -1 ~25mg·L -1 .

[0037] As a preferred embodiment, the concentration of GA solution is 120 mg·L -1 ~500mg·L -1 .

[0038] As a preferred method, the concentration of 6-BA solution is 18 mg·L -1 ~85mg·L -1 .

[0039] As a preferred embodiment, the concentration of IAA solution is 95 mg·L -1 ~305mg·L -1 .

[0040] In this embodiment, the present invention uses beech seeds as research objects to compare the effects of five exogenous hormones, including 6-BA (6-benzyladenine), GA (gibberellic acid), IAA (indoleacetic acid), BR (brassinosteroids), and ABA (abscisic acid), at different concentrations on the germination of beech seeds.

[0041] Table 1 Hormone concentrations

[0042]

[0043] Sterilize the beakers, tweezers, and germination boxes required for the experiment in an autoclave. Also sterilize the seeds. Then, soak the seeds in five different hormone concentrations, using sterile water as a control. Soak for 1 hour, with 100 seeds per treatment, repeated three times. Place the hormone-treated seeds neatly in a 10 cm x 10 cm germination box lined with sterile water-soaked cotton wool, and label them. Place in a 24°C constant temperature light box and water daily to ensure the cotton wool is moist.

[0044] like Figures 2 to 6 As shown in the figure, the seeds of big-leaf beech were cultured for 40 days. The number of germinations of each treatment was counted at 30 days, 35 days, and 40 days, and the germination rate was calculated. When the seeds of big-leaf beech were germinated for 30 days, the concentration of BR group was 10 mg·L-1 The germination rate of the treatment with ABA was the highest, reaching 45.33%. The lowest germination rate was in the ABA group with a concentration of 150 mg·L -1 The germination rate of the control group CK was 29.33%. When the seeds of big-leaf beech germinated for 35 days, the concentration of BR group was 10 mg·L -1 The germination rate of the treatment with ABA was the highest, reaching 55.33%. The lowest germination rate was in the ABA group with a concentration of 150 mg·L -1 The germination rate of the control group CK was 38.67%. When the seeds of big leaf beech germinated for 40 days, the concentration of BR group was 10mg·L -1 The germination rate of the treatment with ABA was the highest, reaching 67.67%. The lowest germination rate was in the ABA group with a concentration of 150 mg·L -1 treatment, was 30.00%; the germination rate of the control group CK was 49.00%.

[0045] The BR hormone treatment group was treated with 10 mg·L -1 , 20mg·L -1 When the concentration was 30 mg·L -1 The 6-BA hormone treatment group has an inhibitory effect on the germination of big-leaf beech seeds. The GA3 hormone treatment group has an inhibitory effect on the germination of big-leaf beech seeds. -1 , 500mg·L -1 When the concentration was 250 mg·L -1 The overall concentration of IAA hormone treatment group has a promoting effect on the germination of big-leaf beech seeds. The overall concentration of ABA hormone treatment group has an inhibitory effect on the germination of big-leaf beech seeds. The concentration of ABA treatment group is 150mg·L -1 The inhibitory effect on the germination of Zelkova spp. seeds was the most serious when the concentration of BR treatment group was 10 mg·L -1 The effect of promoting the germination of big-leaf beech seeds is most obvious.

[0046] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A seedling raising method for improving the germination rate of Zelkova truncatula seeds, characterized in that: Including steps: S1: Seed screening: Select plump, disease-free and insect-free big-leaf beech seeds and rinse them with water to remove empty shelled seeds floating on the water surface; S2: Seed disinfection: disinfect with 10% NaClO solution for 10 minutes, shaking continuously; after disinfection, rinse with sterile water 7-8 times until there is no NaClO residue on the seed surface; S3: Hormone soaking treatment: the sterilized big-leaf beech seeds were placed in the hormone solution and soaked for 1 hour; S4: Wash the seeds 5-6 times with sterile water; S5: Place the hormone-treated seeds in a germination box with a size of 10 cm × 10 cm and lined with absorbent cotton soaked in sterile water; Then place the cotton germination box in a constant temperature and light box at 24℃ and water it every day to ensure that the cotton is moist.

2. The method for raising seedlings for improving the germination rate of Zelkova spp. seeds according to claim 1, wherein: Hormone solutions include BR, GA, 6-6-BA, or IAA.

3. The method for raising seedlings for improving the germination rate of Zelkova spp. seeds according to claim 2, wherein: The concentration of BR solution is 8 mg·L -1 ~25mg·L -1 .

4. The method for raising seedlings for improving the germination rate of Zelkova truncatula seeds according to claim 2, wherein: The concentration of GA solution is 120 mg·L -1 ~500mg·L -1 .

5. The method for raising seedlings for improving the germination rate of Zelkova truncatula seeds according to claim 2, wherein: The concentration of 6-BA solution is 18 mg·L -1 ~85mg·L -1 .

6. The method for raising seedlings for improving the germination rate of Zelkova truncatula seeds according to claim 2, wherein: The concentration of IAA solution is 95 mg·L -1 ~305mg·L -1 .

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