Method for cultivating Ningline No.3 line pepper

Abstract

The invention provides a cultivation method of Ning line No.3 line pepper, and belongs to the technical field of crop breeding. The Ning line No.3 line pepper which is high in yield, strong in stress resistance, good in continuous fruit setting property, excellent in fruit quality, strong in peppery taste and obvious in strain advantage is cultivated through cross breeding. The botanical characteristics of the Zhongning line No.3 line pepper are that: the Zhongning line pepper is late-matured, the plant height is 101.30 cm, the plant width is 76.60 cm, and the plant type is upright. The fruit is linear, the length of the fruit is 30.10 cm, the transverse diameter of the fruit is 1.87 cm, the pulp thickness is 0.20 cm, the weight of a single fruit is 24.30 g, the fruit number of a single plant is 40, a commercial fruit is red, and the flowering and fruiting period is 25 days.

Description

Technical Field

[0001] The present invention relates to the field of breeding technology, and in particular to a method for cultivating Ningxian No. 3 pepper. Background Art

[0002] The fruit of bell peppers is slender and linear, hence the name. Bell peppers are rich in nutrients, including a good source of vitamin C, protein, dietary fiber, and minerals. They are often dark green or bright red (depending on variety and maturity), have a moderately spicy flavor, and are delicious, making them popular with consumers and in high demand.

[0003] However, existing pepper germplasm resources have limitations in terms of disease resistance, stress tolerance, yield and quality, making it difficult to meet market demand. Therefore, breeding a batch of new pepper varieties with good resistance and high yield is crucial to promoting the development of the pepper industry. Summary of the Invention

[0004] The object of the present invention is to provide a method for cultivating Ningxian No. 3 pepper, which can be used to cultivate Ningxian No. 3 pepper with high yield, strong stress resistance, good continuous fruit setting, excellent fruit quality, strong spicy taste and obvious variety advantages.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The present invention provides a method for cultivating Ningxian No. 3 pepper, comprising the following steps:

[0007] (1) Breeding of female parent: Pepper seeds from Zunyi, Guizhou Province were hybridized with high-generation homozygous Yinchuan Yangjiao pepper to obtain hybrids. The seeds of the hybrids were self-pollinated for 6 generations using the pedigree method, and field stress screening and self-pollination purification were performed to obtain the female parent 20221.

[0008] (2) Male parent breeding: 20 pepper germplasm resources were introduced from Zunyi, Guizhou. In the same year, they were hybridized with the high-generation homozygous Yinchuan Yangjiao pepper in a solar greenhouse. After screening and self-pollination purification, the male parent 2077 was obtained.

[0009] (3) Cultivation of Ningxian No. 3 pepper: The female parent 20221 obtained in step (1) was hybridized with the male parent 2077 obtained in step (2) to obtain Ningxian No. 3 pepper.

[0010] Furthermore, in step (1), the properties of the female parent 20221 are:

[0011] Medium maturity, full growth cycle of 190 days, plant height 60-70 cm, strong resistance to adversity, single plant with 40-50 fruits, the first female flower node is 7 or 8, the fruit is long and thin horn-shaped with shallow wrinkles, young fruit is green, mature fruit is red, with strong spicy taste, average single fruit weight is 26.6 grams.

[0012] Furthermore, the screening conditions for the hybrids obtained in step (1) are: good resistance, long fruit shape, strong spicy taste and uniform upper and lower fruit shape.

[0013] Furthermore, the characteristics of the male parent 2077 in step (2) are: late maturity, a full growth cycle of 210 days, a plant height of 85-95 cm, strong stress resistance, 50-55 fruits per plant, 11 first female flower nodes, long linear fruits without wrinkles, green young fruits, red mature fruits, strong spicy flavor, and an average single fruit weight of 9.5 grams.

[0014] The beneficial effects of the present invention compared with the prior art are

[0015] (2) The present invention cultivates Ningxian No. 3 pepper through hybrid breeding, which has high yield, strong stress resistance, good continuous fruit setting, excellent fruit quality, strong spicy taste and obvious variety advantages.

[0016] (3) The botanical characteristics of the pepper of Ningxian No. 3 of the present invention are:

[0017] Late-maturing, with an upright plant shape, 101.30 cm tall and 76.60 cm wide. The fruit is linear, 30.10 cm long, 1.87 cm wide, and 0.20 cm thick. Each fruit weighs 24.30 g and produces 40 fruits per plant. The commercial fruit is red, with a flowering and fruiting period of 25 days. DETAILED DESCRIPTION

[0018] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0019] It should be understood that the terms described herein are intended only to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges herein, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between any intermediate value within a stated value or stated range and any other stated value or intermediate value within the stated range is also encompassed by the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded within the scope.

[0020] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0021] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be exemplary only.

[0022] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0023] The pepper germplasm resources used in the present invention are shown in Table 1, which researchers can purchase or apply for from the corresponding sources:

[0024] Table 1 Pepper germplasm resources

[0025]

[0026]

[0027] Example 1

[0028] Example 1 of the present invention provides a method for cultivating Ningxian No. 3 pepper, the specific steps are as follows:

[0029] (1) Female parent selection

[0030] In January 2016, 20 pepper germplasm resources were introduced from Zunyi, Guizhou (see Table 1). In the same year, they were hybridized with the high-generation homozygous Yinchuan Yangjiao pepper in a solar greenhouse, and 13 hybrids with good resistance, long fruit shape, strong spicy flavor and uniform upper and lower fruit shape were selected.

[0031] From 2016 to 2019, these hybrids were planted in the comprehensive experimental base of Ningxia Academy of Agricultural and Forestry Sciences, the base of Zhejiang University Hainan Research Institute and the solar greenhouse, and planted for 6 generations according to the pedigree method, and field stress screening and self-pollination purification were carried out.

[0032] In 2020, 37 stable and excellent inbred lines were selected and retained. The line with the characteristics of mid-ripening, full growth cycle of 190 days, plant height of 60-70 cm, strong stress resistance, 40-50 fruits per plant, 7 or 8 nodes of the first female flower, long and slender horn-shaped fruits with shallow wrinkles, green young fruits, red mature fruits, strong spicy taste, and an average single fruit weight of 26.6 grams was used as the female parent and named 20221.

[0033] (2) Male parent selection

[0034] The breeding process is similar to that of the female parent. In January 2016, 20 pepper germplasm resources were introduced from Zunyi, Guizhou (see Table 1). In the same year, they were hybridized with the high-generation homozygous Yinchuan Yangjiao pepper in a solar greenhouse. After screening, multi-generation planting, adversity screening and self-pollination purification, 37 stable excellent inbred lines were selected in 2020. The line is characterized by late maturity, a full growth cycle of 210 days, a plant height of 85-95 cm, strong stress resistance, 50-55 fruits per plant, the first female flower node is 11, the fruit is long and linear, without wrinkles, the young fruit is green, the mature fruit is red, the spicy taste is strong, and the average single fruit weight is 9.5 grams. It is the male parent and named 2077.

[0035] (3) Hybrid combination preparation and testing

[0036] In June 2020, the hybrid combination 20221×2077 was formulated at the Comprehensive Experimental Base of Ningxia Academy of Agricultural and Forestry Sciences, and the first-generation hybrid seeds were harvested in September 2020.

[0037] In 2021, combination variety comparison tests were carried out in the open fields of Yinchuan and Pengyang, Ningxia, showing the characteristics of high yield, excellent quality, and strong disease resistance (in the Pengyang open field variety comparison test, the average number of fruits per plant reached more than 40, and the yield reached more than 1,800 kilograms. The quality was excellent, the spicy taste was strong, and there was basically no incidence of pepper virus disease and anthracnose).

[0038] In 2022-2023, multi-point regional trials and production trials were carried out in Yinchuan, Zhongwei, Pingluo, Pengyang and other places in Ningxia. The yield increased by 10.02%-29.72% compared with the control variety Screw Long Line. It has strong adaptability in the field, stable traits, strong stress resistance, good continuous fruit setting, excellent fruit quality, strong spicy taste, and obvious variety advantages. It was named Ningxian No. 3 in 2023.

[0039] Botanical characteristics of Ningxian No. 3

[0040] (1) Late-maturing, plant height 101.30 cm, plant width 76.60 cm, upright plant. Fruit is linear, 30.10 cm long, 1.87 cm transverse diameter, 0.20 cm flesh thickness, single fruit weight 24.30 g, 40 fruits per plant, commercial fruit red, flowering and fruiting period 25 days.

[0041] (2)Quality characteristics

[0042] Vitamin C content is 143mg / 100g, and capsaicin content is 0.460%.

[0043] (3) Stress resistance

[0044] It has strong drought resistance, heat resistance and salt-alkali resistance, moderate resistance to viral diseases (CMV, TMV), high resistance to blight and moderate resistance to anthrax.

[0045] (4) Production performance

[0046] The yield per mu in the first growth cycle was 1928.58 kg, an increase of 20.80% over the control long screw line; the yield per mu in the second growth cycle was 1993.76 kg, an increase of 19.12% over the control long screw line.

[0047] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A method for cultivating Ningxian No. 3 pepper, characterized in that: The steps include: (1) Breeding of female parent: Pepper seeds from Zunyi, Guizhou were hybridized with high-generation homozygous Yinchuan Yangjiao pepper to obtain hybrids. The seeds of the hybrids were self-pollinated for 6 generations using the pedigree method, and field stress screening and self-pollination purification were performed to obtain the female parent 20221. (2) Male parent breeding: 20 pepper germplasm resources were introduced from Zunyi, Guizhou. In the same year, they were hybridized with the high-generation homozygous Yinchuan Yangjiao pepper in a solar greenhouse. After screening and self-pollination purification, the male parent 2077 was obtained. (3) Cultivation of Ningxian No. 3 pepper: The female parent 20221 obtained in step (1) was hybridized with the male parent 2077 obtained in step (2) to obtain Ningxian No. 3 pepper.

2. The method for cultivating the Ningxian No. 3 pepper according to claim 1, wherein: In step (1), the properties of the female parent 20221 are: Medium maturity, full growth cycle of 190 days, plant height 60-70 cm, strong resistance to adversity, single plant with 40-50 fruits, the first female flower node is 7 or 8, the fruit is long and thin horn-shaped with shallow wrinkles, young fruit is green, mature fruit is red, with strong spicy taste, average single fruit weight is 26.6 grams.

3. The method for cultivating the Ningxian No. 3 pepper according to claim 1, wherein The screening conditions for the hybrids obtained in step (1) are: good resistance, long fruit shape, strong spicy taste and uniform upper and lower fruit shape.

4. The method for cultivating the Ningxian No. 3 pepper according to claim 1, wherein The characteristics of the male parent 2077 described in step (2) are: late maturity, full growth cycle of 210 days, plant height of 85-95 cm, strong stress resistance, 50-55 fruits per plant, the first female flower node is 11, the fruit is long linear, without wrinkles, the young fruit is green, the mature fruit is red, the spicy flavor is strong, and the average single fruit weight is 9.5 grams.

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