Anti-blood coagulation device for mice

Abstract

The invention relates to the technical field of animal experiment devices, and discloses a mouse blood coagulation preventing device which comprises a plurality of mouse fixing recognition and blood sampling devices, a mouse blood typing instrument, a vortex system, a blood waste liquid collecting device, an anticoagulant premixing system, a micro pump, a plurality of optical sensors and a blood refrigerating box. The mouse fixing recognition and blood sampling device is connected with the mouse blood typing instrument through a pipeline, the mouse blood typing instrument is connected with the vortex system and the blood waste liquid collecting device through pipelines, and the vortex system is connected with the micro pump and the optical sensor through pipelines. The micro pump is connected with the anticoagulant premixing system through a pipeline, and the optical sensor is connected with the blood refrigerating box through a pipeline; the device is high in efficiency, manual operation can be avoided, mechanical damage, pollution in the experiment process and animal stress are reduced, and the possibility of blood coagulation is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of animal experimental devices, in particular to a mouse anti-coagulation device. Background Art

[0002] Currently, mouse blood collection is usually performed manually by experimenters, and human errors are prone to occur during this process, making blood collection difficult.

[0003] During the operation, mechanical damage may be caused by the operator's operation, such as blood oscillation, excessive mixing speed, etc., which may lead to blood coagulation; During the operation, uneven anticoagulant concentrations may cause a decrease in cell membrane stability, leading to coagulation; During the operation, the temperature is difficult to control, which may damage the red blood cell structure and cause coagulation; During the operation, due to the long processing time, complement components are activated and metabolites accumulate, leading to coagulation; Mixing blood from different mice will cause coagulation due to differences in blood types and blood surface antigens. Summary of the Invention

[0004] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a mouse anti-coagulation device that can solve the problems of long time, large error and instability in manual blood collection from mice, and easy coagulation of mixed mouse blood.

[0005] To achieve the above object, the present invention provides the following technical solutions: The present invention provides a mouse anti-coagulation device, comprising a plurality of mouse fixed identification and blood collection devices, a mouse blood typing instrument, a vortex system, a blood waste liquid collection device, an anticoagulant premixing system, a micro pump, a plurality of optical sensors and a blood refrigerator; the mouse fixed identification and blood collection devices are connected to the mouse blood typing instrument via a pipeline, the mouse blood typing instrument is respectively connected to the vortex system and the blood waste liquid collection device via a pipeline, the vortex system is respectively connected to the micro pump and the optical sensor via a pipeline, the micro pump is connected to the anticoagulant premixing system via a pipeline, and the optical sensor is connected to the blood refrigerator via a pipeline.

[0006] Furthermore, the mouse fixation, identification and blood collection device includes a mouse fixator, a mouse blood collection tube, an infrared meter and a robotic arm. The infrared meter is connected to the mouse fixator and the robotic arm. The robotic arm is provided with a blood collection needle. The mouse fixator is provided with four mouse limb fixing clamps. The mouse fixator is also provided with a heating pad. A nanofiber membrane is installed in the mouse blood collection tube. The mouse fixator is used to fix the mouse and collect the collected mouse blood into the mouse blood collection tube. The infrared meter identifies the mouse and the robotic arm drives the blood collection needle into the submandibular position of the mouse.

[0007] Furthermore, the mouse blood typing instrument includes a base, on which are fixed a plurality of support rods, the tops of the plurality of support rods being connected to fixed rods, each of the support rods being provided with a microtube fixing clamp, on which a microtube is fixedly mounted, a plurality of substrates being provided on the base, on which different antibodies are divided by region, a blood typing instrument display screen being also provided on the base for displaying blood analysis results, the mouse fixing identification and blood collection device being connected to the microtube via a pipeline, and an enzyme marker being connected to the base of the blood typing instrument for checking the absorbance value of each substrate.

[0008] Furthermore, the anticoagulant premix system includes an anticoagulant storage chamber, multiple anticoagulant tubes, a premix container fixing box, multiple premix containers and an oscillator. Multiple anticoagulant tubes are fixed in the anticoagulant storage chamber, multiple premix containers are fixed in the premix container fixing box, the oscillator is connected to the premix container fixing box, a valve is installed on the anticoagulant tube, the anticoagulant tube is connected to the premix container through a pipeline, and the premix container is connected to the micro pump.

[0009] Furthermore, the vortex system includes three vortex instruments, each of which includes a square housing, a power machine, a rotating shaft, a cylindrical container and a display screen. The cylindrical container is installed in the square housing, the power machine is connected to the cylindrical container through the rotating shaft, the display screen is installed on the outside of the square housing, and is used to display the vortex speed, internal temperature and the status of the vortex instrument. The cylindrical container is connected to the micro pump, the mouse blood typing instrument is connected to the cylindrical container, and the cylindrical container in each vortex instrument is respectively connected to one of the optical sensors.

[0010] Furthermore, the number of the optical sensors is the same as the number of the vortex meters.

[0011] Furthermore, the blood cold storage box includes a blood cold storage chamber, a temperature control component, a temperature display screen, a cold storage chamber cover and multiple blood collection tubes. The blood collection tubes are fixedly installed in the blood cold storage chamber, the temperature control component is installed in the blood cold storage chamber, the cold storage chamber cover is located above the blood cold storage chamber, the temperature display screen is installed on the outside of the blood cold storage chamber, and the optical sensor is connected to the blood collection tubes.

[0012] Furthermore, it also includes an intelligent control system and a disinfection system. The disinfection system, multiple mouse fixation identification and blood collection devices, a mouse blood typing instrument, a vortex system, a blood waste collection device, an anticoagulant premixing system, a micropump, multiple optical sensors and a blood refrigerator are all electrically connected to the intelligent control system, and the mouse coagulation device is disinfected by the disinfection system.

[0013] The present invention has the following beneficial effects: 1. The present invention is highly efficient and can trace experimental samples according to the results from the control system. It can also process multiple mice at the same time, which can reduce the experimental time and prevent the accumulation of metabolites.

[0014] 2. The present invention adopts automated processing of the control system, which can avoid manual operation, reduce mechanical damage, experimental process pollution and animal stress.

[0015] 3. The present invention sets a heating pad on the mouse fixator, and can reduce the differences in animal blood types and blood surface antigens through a mouse blood typing instrument, thereby reducing the possibility of coagulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the overall structure of a mouse anti-coagulation device of the present invention; Figure 2 This is a schematic structural diagram of the main part of a mouse anti-coagulation device of the present invention; Figure 3 This is a schematic structural diagram of a mouse anti-coagulation device, a mouse fixation identification and blood collection device according to the present invention; Figure 4 This is a schematic structural diagram of an anticoagulant premixing system of a mouse anti-coagulation device of the present invention; Figure 5 This is a schematic structural diagram of a vortex instrument for preventing blood coagulation in mice according to the present invention; Figure 6 This is a schematic structural diagram of a blood refrigerator for mice anti-coagulation device according to the present invention; Figure 7 This is a schematic structural diagram of a mouse blood typing instrument for a mouse anti-coagulation device according to the present invention; Figure 8 This is a schematic structural diagram of a base in a mouse blood typing instrument for a mouse anti-coagulation device according to the present invention; Legend: 1. Mouse fixation, identification, and blood collection device; 2. Mouse blood typing instrument; 3. Vortex system; 4. Blood waste collection device; 5. Anticoagulant premixing system; 6. Micropump; 7. Optical sensor; 8. Blood refrigerator; 101. Mouse fixator; 102. Mouse limb fixator; 106. Heating pad; 201. Mouse blood collection tube; 202. Nanofiber membrane; 301. Anticoagulant storage chamber; 302. Anticoagulant tube; 309. Valve; 310. Oscillator; 312. Premix Container fixing box; 313, premix container; 401, square shell; 402, power machine; 403, rotating shaft; 404, cylindrical container; 405, display screen; 501, blood cold storage chamber; 502, temperature control component; 503, temperature display screen; 504, cold storage chamber cover; 505, blood collection tube; 601, fixing rod; 602, support rod; 603, micro tube fixing clamp; 604, micro tube; 605, base; 606, blood typing instrument display screen; 607, base. DETAILED DESCRIPTION

[0017] The following will provide a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0018] Reference Figure 1-8 The present invention provides an embodiment: a mouse anti-coagulation device, including six mouse fixation identification and blood collection devices 1, a mouse blood typing instrument 2, a vortex system 3, a blood waste liquid collection device 4, an anticoagulant premixing system 5, a micro pump 6, three optical sensors 7 and a blood refrigerator 8. The mouse fixation, identification and blood collection device 1 includes a mouse fixer 101 and a mouse blood collection tube 201, and also includes an infrared meter and a robotic arm. The infrared meter is connected to the mouse fixer 101 and the robotic arm. The robotic arm is provided with a blood collection needle. The mouse fixer 101 is provided with four mouse limb fixing clamps 102. The mouse fixer 101 is also installed with a heating pad 106 to prevent coagulation problems caused by temperature during mouse blood collection. A nanofiber membrane 202 is installed in the mouse blood collection tube 201. The mouse fixer 101 is used to fix the mouse. The infrared meter identifies the mouse. The robotic arm drives the blood collection needle into the submandibular position of the mouse. The collected mouse blood is filtered through the nanofiber membrane 202 by the blood collection needle and collected into the mouse blood collection tube 201.

[0019] The mouse blood typing instrument includes a base 607, on which six support rods 602 are fixedly mounted. The tops of the six support rods 602 are connected to fixing rods 601. Each support rod 602 has a corresponding microtube fixing clamp 603, which is used to secure a microtube 604. The mouse blood typing instrument base 607 includes multiple bases 605, each of which is divided into regions containing different antibodies. After mouse blood enters the microtube 603, it drips into the bases 605 at a constant flow rate, reacting with different antibodies. If the bases 605 change color, the mouse's blood type is determined. The mouse blood typing instrument base 607 also includes a blood typing instrument display 606, which displays the blood analysis results for each channel and the operating or rest status of the blood analyzer. The mouse blood typing instrument is also connected to a microplate reader to measure the OD (absorbance) value of the bases 605, which is used to determine the degree of hemolysis in the blood. After the microplate reader detects that there is no hemolysis, the blood enters the vortex system 3.

[0020] The anticoagulant premix system 5 includes an anticoagulant storage chamber 301, multiple anticoagulant tubes 302, a premix container fixing box 312, three premix containers 313 and an oscillator 310. Multiple anticoagulant tubes 302 are fixed in the anticoagulant storage chamber 301, three premix containers 313 are fixed in the premix container fixing box 312, the oscillator 310 is connected to the premix container fixing box 312, each anticoagulant tube 302 is installed with a valve 309, the anticoagulant tube 302 is connected to the premix container 313 through a pipeline, the premix container 313 is connected to the micro pump 6, and different anticoagulants are placed in the multiple anticoagulant tubes 302.

[0021] The vortex system 3 includes three vortex instruments, each of which includes a square housing 401, a power machine 402, a rotating shaft 403, a cylindrical container 404 and a display screen 405. The cylindrical container 404 is installed in the square housing 401, and the power machine 402 is connected to the cylindrical container 403 through the rotating shaft 403. The display screen 405 is installed on the outside of the square housing 401. The display screen 405 displays the vortex speed, internal temperature and normal or abnormal status of the vortex instrument. The cylindrical container 404 is connected to the micro pump 6, and the mouse blood typing instrument 2 is connected to the cylindrical container 404. The cylindrical container 404 in each vortex instrument is respectively connected to one of the optical sensors 7, and is connected to the blood refrigerator 8 through the optical sensor 7. The optical sensor 7 is inserted between the vortex system 3 and the blood refrigerator 8 to detect the fluidity of the blood in real time and whether there is blood coagulation.

[0022] Due to the varying requirements of later blood experiments, the choice, ratio, and concentration of anticoagulants vary. Furthermore, attention should be paid to the synergistic and cross-influence effects of different anticoagulants. When blood is drawn from mice, the anticoagulant selection and concentration requirements are input into the control system. Multiple solutions are drawn into the anticoagulant premixing system 5 via a micropump 6, mixed evenly, and then fed into the different vortexers in the vortex system.

[0023] The number of the optical sensors 7 is the same as the number of the vortex meters.

[0024] The blood cold storage box 8 includes a blood cold storage chamber 501, a temperature control component 502, a temperature display screen 503, a cold storage chamber cover 504, multiple blood collection tubes 505 and an alarm system. The blood collection tubes 505 are fixedly installed in the blood cold storage chamber 501, the temperature control component 502 and the alarm system are installed in the blood cold storage chamber 501, the cold storage chamber cover 504 covers the top of the blood cold storage chamber 501, the temperature display screen 503 is installed on the outside of the blood cold storage chamber 501, the optical sensor 7 is connected to the blood collection tubes 505, and the temperature changes in real time will appear on the temperature display screen 503. When the temperature exceeds 4°C±0.5°C, the alarm system is triggered to ensure the storage temperature of the blood.

[0025] The mouse anti-coagulation device also includes an intelligent control system and a disinfection system. The disinfection system, multiple mouse fixed identification and blood collection devices 1, a mouse blood typing instrument 2, a vortex system 3, a blood waste liquid collection device 4, an anticoagulant premixing system 5, a micro pump 6, multiple optical sensors 7 and a blood refrigerator 8 are all electrically connected to the intelligent control system. The mouse coagulation device is disinfected by the disinfection system. The disinfection system runs through each instrument and equipment of the mouse anti-coagulation device, and the disinfection system will be automatically turned on before and after the entire process starts and ends.

[0026] Working principle: The whole process is automatically monitored by the intelligent control system. After the device is turned on, the disinfection system is automatically started, and each pipe is flushed with PBS for wetting. The mouse blood collection volume is set in the intelligent control system, the selection and concentration ratio of different anticoagulants are set, and the vortex parameters of the vortex system are set. After the preparation is completed, the mouse fixation, identification and blood collection device 1 and the anticoagulant premixing system 5 are started at the same time. The mouse is fixed in the mouse fixator 101, and the limbs are fixed by the mouse limb fixing clamps 102. The infrared device identifies the mouse, and the robotic arm drives the blood collection needle into the submandibular position of the mouse. The blood is filtered through the nanofiber membrane 202 and flows into the mouse blood collection tube 201. At the same time, the pipeline connecting the blood collection tube and the mouse blood typing instrument automatically draws blood into the mouse blood typing instrument 2 for blood testing. Blood with the same blood type / surface antigen enters the same vortex instrument in the vortex system 3. If the blood is detected to have a blood disease, it enters the blood waste liquid collection device 4. Multiple anticoagulant tubes 302 open valves 309 according to pre-set parameters and enter different premix containers 313. An oscillator 310 begins to vibrate to evenly mix the anticoagulant. After uniform mixing, the blood is drawn into the pre-set vortex system 3 via a micropump 6, where it is thoroughly mixed with the blood. The blood and anticoagulant pipes flow directly into the cylindrical container 404 inside the vortex vortexer. When the blood stops flowing, the motor 402 drives the rotating shaft 403 to initiate vortexing. The display screen 405 displays the vortex parameters and operating status. After thorough mixing, the blood enters the blood refrigerator 8 via an optical sensor 7. The optical sensor 7 monitors the fluidity of the blood and triggers an alarm system if blood is not flowing. If the blood is flowing normally, it enters the individual blood collection tubes 505 in the blood refrigerator 501. A temperature control unit 502 is also installed inside the blood refrigerator 501. The temperature display screen 503 displays the temperature in real time. If the temperature exceeds 4°C ± 0.5°C, an alarm system is triggered to ensure the blood is stored at the correct temperature. Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A mouse anti-coagulation device, characterized in that: The device comprises a plurality of mouse fixed identification and blood collection devices, a mouse blood typing instrument, a vortex system, a blood waste liquid collection device, an anticoagulant premixing system, a micro pump, a plurality of optical sensors and a blood refrigerator; the mouse fixed identification and blood collection devices are connected to the mouse blood typing instrument via a pipeline, the mouse blood typing instrument is respectively connected to the vortex system and the blood waste liquid collection device via a pipeline, the vortex system is respectively connected to the micro pump and the optical sensor via a pipeline, the micro pump is connected to the anticoagulant premixing system via a pipeline, and the optical sensor is connected to the blood refrigerator via a pipeline.

2. The mouse anti-coagulation device according to claim 1, characterized in that: The mouse fixation, identification and blood collection device includes a mouse fixator, a mouse blood collection tube, an infrared meter and a robotic arm. The infrared meter is connected to the mouse fixator and the robotic arm. The robotic arm is provided with a blood collection needle. The mouse fixator is provided with four mouse limb fixing clamps. The mouse fixator is also installed with a heating pad. A nanofiber membrane is installed in the mouse blood collection tube. The mouse fixator is used to fix the mouse and collect the collected mouse blood into the mouse blood collection tube. The infrared meter identifies the mouse and the robotic arm drives the blood collection needle into the submandibular position of the mouse.

3. The mouse anti-coagulation device according to claim 1, characterized in that: The mouse blood typing instrument includes a base, on which a plurality of support rods are fixed, and the tops of the plurality of support rods are connected to fixed rods. Each of the support rods is provided with a microtube fixing clamp, and the microtube is fixedly installed on the microtube fixing clamp. The base is provided with a plurality of substrates, and different antibodies are divided into regions on the substrates. The base is also provided with a blood typing instrument display screen for displaying blood analysis results. The mouse fixation identification and blood collection device is connected to the microtubes via a pipeline. The base of the blood typing instrument is connected to an enzyme marker for checking the absorbance value of each substrate.

4. The mouse anti-coagulation device according to claim 1, characterized in that: The anticoagulant premix system includes an anticoagulant storage chamber, multiple anticoagulant tubes, a premix container fixing box, multiple premix containers and an oscillator. Multiple anticoagulant tubes are fixed in the anticoagulant storage chamber, multiple premix containers are fixed in the premix container fixing box, the oscillator is connected to the premix container fixing box, a valve is installed on the anticoagulant tube, the anticoagulant tube is connected to the premix container through a pipeline, and the premix container is connected to the micro pump.

5. The mouse anti-coagulation device according to claim 1, characterized in that: The vortex system includes three vortex instruments, each of which includes a square shell, a power machine, a rotating shaft, a cylindrical container and a display screen. The cylindrical container is installed in the square shell, the power machine is connected to the cylindrical container through the rotating shaft, the display screen is installed on the outside of the square shell, and is used to display the vortex speed, internal temperature and the status of the vortex instrument. The cylindrical container is connected to the micro pump, and the mouse blood typing instrument is connected to the cylindrical container. The cylindrical container in each vortex instrument is respectively connected to one of the optical sensors.

6. The mouse anti-coagulation device according to claim 5, characterized in that: The number of the optical sensors is the same as the number of the vortex meters.

7. The mouse anti-coagulation device according to claim 1, characterized in that: The blood cold storage box includes a blood cold storage chamber, a temperature control component, a temperature display screen, a cold storage chamber cover, and multiple blood collection tubes. The blood collection tubes are fixedly installed in the blood cold storage chamber, the temperature control component is installed in the blood cold storage chamber, the cold storage chamber cover is located above the blood cold storage chamber, the temperature display screen is installed on the outside of the blood cold storage chamber, and the optical sensor is connected to the blood collection tubes.

8. The mouse anti-coagulation device according to claim 1, characterized in that: It also includes an intelligent control system and a disinfection system. The disinfection system, multiple mouse fixation identification and blood collection devices, a mouse blood typing instrument, a vortex system, a blood waste collection device, an anticoagulant premixing system, a micropump, multiple optical sensors and a blood refrigerator are all electrically connected to the intelligent control system, and the mouse coagulation device is disinfected by the disinfection system.

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