Compost for increasing yield of double-cropping black raspberries, preparation method and application

Abstract

The invention discloses compost for increasing the yield of double-cropping black raspberries, a preparation method and application. The compost is prepared from the following raw materials in percentage by weight: 25%-30% of wheat straw, 20%-30% of rice straw, 5%-20% of fallen leaves and weeds, 5%-15% of livestock manure, 15%-32% of livestock manure and 3%-5% of bacterial liquid. The sum of all the components is 100%. Wherein the poultry manure comprises 5-10% of chicken manure and 0-5% of goose manure; the livestock manure comprises 5-17% of sheep manure, 0-5% of pig manure and 0-10% of cow dung; the bacterial liquid comprises water, brown sugar, milk powder, sodium chloride and strain fermentation liquor; the strain fermentation liquor comprises streptococcus thermophilus liquor, aspergillus oryzae liquor, compost pseudomonas liquor, bacillus megatherium liquor, bacillus thuringiensis subsp. Kenya and bacillus subtilis liquor. According to the technical scheme, the mixed bacterial suspension is prepared by combining various dominant bacteria, so that the effects of improving soil organic matters, improving the nutritional level of fruit trees, increasing the resistance of the fruit trees and inhibiting diseases and insect pests are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological fertilizers, and in particular to a compost for increasing the yield of double-season black raspberries, a preparation method and an application thereof. Background Art

[0002] Black raspberry is a perennial deciduous shrub in the genus Rubus in the Rosaceae family. In Chinese medicinal texts, raspberries are referred to as 'raspberries,' which are categorized by color as black raspberries, yellow raspberries, purple raspberries, and black raspberries. Black raspberries are highly nutritious, containing high levels of ellagic acid, raspberry ketones, and anthocyanins. They not only possess high nutritional value but also enhance insulin secretion, scavenge free radicals, activate skin sensory neurons, promote hair growth, and have a therapeutic effect on diabetes and arteriosclerosis.

[0003] Currently, there is no mature experience in organic double-season black raspberry cultivation in my country. Black raspberries often experience excessive stem sprouting, enlarged leaves, and excessive basal branching during their growth, requiring high soil fertility. By the second year after planting, they experience problems such as yellowing and curling leaves, low fruit set, and small fruit. Management methods such as improved irrigation, manual weeding, and heavy pruning have been ineffective in improving the growth of black raspberries. However, organic black raspberry cultivation utilizes efficient composting, allowing the plants to quickly and effectively absorb minerals, which not only improves growth but also increases fruit quality and yield.

[0004] With the gradual development of composting, a large number of biofertilizers are now available on the market, most of which are composite strains with a wide variety of strains. During use, problems such as insufficient fermentation of the fertilizer substrate, poor absorption by fruit trees, insignificant growth improvement, and improper fertilizer application methods have occurred. These problems can lead to poor growth of fruit trees. In addition, different fruit trees have different microecological environments and soil microbial composition. When using commercially available composite fertilizers, fruit trees may wilt, leaves may turn yellow and sunburned, and suffer from severe pests and diseases. The optimal biofertilizer for different fruit tree varieties also varies. Therefore, how to develop a biofertilizer suitable for double-season black raspberry production that can help increase black raspberry yield and provide pest and disease control is an urgent problem that needs to be solved by those skilled in the art. Summary of the Invention

[0005] The technical solution of the present invention to solve the above technical problems is to provide a compost for increasing the production of double-season black raspberries, which is composed of the following raw materials in percentage by weight:

[0006] Wheat straw 25%-30%, rice straw 20%-30%, fallen leaves and weeds 5%-20%, poultry manure 5%-15%, livestock manure 15%-32%, bacterial solution 3%-5%; the sum of all ingredients is 100%;

[0007] Wherein, the poultry manure includes 5%-10% chicken manure and 0-5% goose manure;

[0008] The livestock manure includes 5%-17% sheep manure, 0-5% pig manure and 0-10% cow manure;

[0009] The bacterial liquid comprises: water, brown sugar, milk powder, sodium chloride, and bacterial fermentation liquid;

[0010] The bacterial fermentation liquid includes: thermophilic streptococcus liquid, aspergillus oryzae liquid, composting pseudomonas liquid, bacillus megaterium liquid, bacillus thuringiensis subspecies kenya, and bacillus subtilis liquid.

[0011] Furthermore, the weight percentage of each bacterial species in the bacterial fermentation liquid is: 20%-25% of thermophilic Streptococcus liquid, 15%-20% of Aspergillus oryzae liquid, 20%-25% of composting Pseudomonas liquid, 10%-12% of Bacillus megaterium liquid, 10%-12% of Bacillus thuringiensis subspecies kenya, and 15%-16% of Bacillus subtilis liquid.

[0012] Furthermore, the weight percentages of the components in the bacterial solution are: water 60%-80%, brown sugar 5%-10%, milk powder 3%-10%, sodium chloride 2%-5%, and bacterial fermentation liquid 10%-25%;

[0013] In order to solve the above technical problems, the present invention further proposes a method for preparing the compost as described above, comprising the following steps:

[0014] Step 1, bacterial activation: Pseudomonas composting, Bacillus subtilis subsp. subtilis, Bacillus megaterium, Streptococcus thermophilus, Aspergillus oryzae, and Bacillus thuringiensis subsp. kenya are inoculated into a dedicated culture medium respectively, and cultured in a constant temperature shaking incubator;

[0015] Among them, Bacillus thuringiensis subspecies Kenya, Bacillus megaterium, Pseudomonas composting, and Bacillus subtilis were cultured at 28°C-30°C for 2-3 days, Aspergillus oryzae was cultured at 23°C-28°C for 4-7 days, and Streptococcus thermophilus was cultured at 42°C-45°C for 2-3 days, and all were subcultured for 2-3 cycles;

[0016] Bacteria acclimation: Add the activated bacterial fermentation liquid to the acclimation medium (containing 60%-80% drinking water, 5%-10% brown sugar, 3% milk powder, and 2% sodium chloride) in proportion, and culture at 28°C-32°C for 2-3 days. For thermophilic Streptococcus, culture at 40°C-45°C for 3-5 days. Subculture for 2-3 cycles.

[0017] Step 2, prepare mixed bacterial solution: mix the acclimated bacterial strains according to the proportion, culture at 30°C for 3 days, and dilute the fermentation solution with water at a ratio of 1:20 for later use;

[0018] Among them, the proportion of the domesticated strains is: 20%-25% of thermophilic Streptococcus liquid, 15%-20% of Aspergillus oryzae liquid, 20%-25% of composting Pseudomonas liquid, 10%-12% of Bacillus megaterium liquid, 10%-12% of Bacillus thuringiensis subspecies Kenya, and 15%-16% of Bacillus subtilis liquid.

[0019] Step 3, material processing: 25%-30% wheat straw, 20%-40% rice straw, and 5%-20% fallen leaves and weeds are crushed and mixed evenly;

[0020] Step 4: Layered composting: Stack in 5 layers, with odd-numbered layers consisting of fallen leaves and weeds, and even-numbered layers consisting of poultry manure and livestock manure. Spray the mixed bacterial solution between each layer, and cover the top with a 1-2 cm layer of soil and plastic film. Ferment for 90-100 days in winter and 60-75 days in summer, turning the pile at least 3 times during this period, and complete fermentation when the temperature drops below 38°C.

[0021] Furthermore, the dedicated culture medium comprises:

[0022] Compost Pseudomonas culture medium: tryptone 15.0 g / L, soytone 5.0 g / L, sodium chloride 5.0 g / L;

[0023] Streptococcus thermophilus culture medium: casein peptone 20 g / L, yeast extract powder 5.0 g / L, glucose 5 g / L, sucrose 5 g / L, lactose 5 g / L, Tween 80 1.0 mL / L, sodium chloride 4.0 g / L, sodium acetate 1.5 g / L, ascorbic acid 0.5 g / L;

[0024] Aspergillus oryzae and Bacillus subtilis culture medium: 1.0 L of 20% potato extract, 20.0 g / L of glucose;

[0025] Bacillus megaterium and Bacillus thuringiensis culture medium: peptone 5.0 g / L, beef extract 3.0 g / L, sodium chloride 5.0 g / L.

[0026] Furthermore, the acclimation culture medium is: 60%-80% drinking water, 5%-10% brown sugar, 3% milk powder, and 2% sodium chloride.

[0027] Furthermore, in step 4, the frequency of turning the compost is: once every 20-25 days in winter and once every 15-20 days in summer.

[0028] Furthermore, the number of viable bacteria in the bacterial fermentation liquid after acclimation is ≥1×10*cfu / ml.

[0029] In order to solve the above technical problems, the present invention also proposes an application of the compost as described above, which is used for the budding period, flowering period, fruit development period, primary stem rapid growth period, and primary stem late development period of double-season black raspberries, characterized in that the fertilizer application rate is 1 ton / mu.

[0030] The beneficial effects of the present invention are:

[0031] (1) After the compost of the present invention is applied, the absorption of minerals by black raspberries is improved, the nutrition of leaves is improved, photosynthesis and nutrient accumulation are enhanced, and the growth potential of fruit trees is also enhanced;

[0032] (2) The present invention combines the fermentation advantages of different strains and adopts a strain domestication method to better retain the fermentation performance of the biofertilizer and the functionality of each strain, thereby improving the adaptability of the strain in the composting stage, ensuring full fermentation, and solving the problem of incomplete fermentation of commercially available bacterial fertilizers;

[0033] (3) The present invention applies fertilizers in five stages, namely, the budding stage, the flowering stage, the fruit development stage, the primary stem rapid growth stage, and the primary stem late development stage, by adopting a multiple and small amount method, thereby improving the fruit quality of the second season of the double-season black raspberry, increasing the fruit yield of a single fruit tree, providing sufficient nutrition for the germinating base plants, and laying the foundation for selecting and retaining fruiting branches in the following year;

[0034] (4) The compost obtained by the present invention has a high organic matter content and is quickly absorbed by fruit trees. The growth potential of fruit trees can be improved the next day after fertilization, and it plays a protective role in improving soil microecology and preventing soil compaction.

[0035] (5) The compost obtained by the present invention has the effect of preventing and controlling pests and diseases, especially the killing effect on nematodes and Lepidoptera and the inhibition effect on mold reproduction, and is more suitable for application in the cultivation of organic black raspberries. DETAILED DESCRIPTION

[0036] The present invention provides a compost for increasing the yield of double-season black raspberries, a preparation method and an application thereof, aiming to develop a biofertilizer suitable for double-season black raspberries, which is beneficial to increasing the yield of black raspberries and having the effect of preventing and controlling diseases and pests.

[0037] The compost for increasing the yield of double-season black raspberries proposed by the present invention will be described in detail below in a specific embodiment:

[0038] Example 1:

[0039] A compost for increasing the yield of double-season black raspberries, comprising the following raw materials in the following weight percentages:

[0040] Wheat straw 25%-30%, rice straw 20%-30%, fallen leaves and weeds 5%-20%, poultry manure 5%-15%, livestock manure 15%-32%, bacterial solution 3%-5%; the sum of all ingredients is 100%;

[0041] Wherein, the poultry manure includes 5%-10% chicken manure and 0-5% goose manure;

[0042] The livestock manure includes 5%-17% sheep manure, 0-5% pig manure and 0-10% cow manure;

[0043] The bacterial liquid comprises: water, brown sugar, milk powder, sodium chloride, and bacterial fermentation liquid;

[0044] The bacterial fermentation liquid includes: thermophilic streptococcus liquid, aspergillus oryzae liquid, composting pseudomonas liquid, bacillus megaterium liquid, bacillus thuringiensis subspecies kenya, and bacillus subtilis liquid.

[0045] Furthermore, the weight percentage of each bacterial species in the bacterial fermentation liquid is: 20%-25% of thermophilic Streptococcus liquid, 15%-20% of Aspergillus oryzae liquid, 20%-25% of composting Pseudomonas liquid, 10%-12% of Bacillus megaterium liquid, 10%-12% of Bacillus thuringiensis subspecies kenya, and 15%-16% of Bacillus subtilis liquid.

[0046] Furthermore, the weight percentages of the components in the bacterial liquid are: water 60%-80%, brown sugar 5%-10%, milk powder 3%-10%, sodium chloride 2%-5%, and bacterial fermentation liquid 10%-25%.

[0047] Fallen leaves and weeds include straw, fallen leaves, and grass. Poultry manure includes chicken, duck, pigeon, and goose manure, while livestock manure includes cow, pig, sheep, horse, and rabbit manure.

[0048] The composting Pseudomonas composti, Bacillus subtilis subsp. subtilis and Bacillus megaterium used are from the China Industrial Microbiological Culture Collection Management Center. The composting Pseudomonas composti has the function of rapid composting and decomposing organic matter. Bacillus subtilis subsp. subtilis has the function of inhibiting mold. The cellulase and α-amylase secreted by it can effectively decompose the high-polymerization degree cellulose and starch in the fertilizer, thereby increasing the organic matter and enhancing the nutrient absorption of fruit trees. The Bacillus megaterium liquid has the function of converting organic phosphorus and improving the phosphorus absorption rate. Streptococcus thermophilus, Aspergillus oryzae, and Bacillus thuringiensis subsp. kenyae are from the Institute of Biology, Gansu Academy of Sciences. Streptococcus thermophilus has stable fermentation activity, has the effect of continuous fermentation and high temperature resistance, Aspergillus oryzae can metabolize protease and amylase, has the effect of decomposing protein and starch, improving soil organic matter and preventing soil compaction, and Bacillus thuringiensis subsp. kenyae has the effect of killing nematodes and insects such as Lepidoptera. The present invention combines the above 6 dominant strains to prepare a mixed bacterial suspension, which improves soil organic matter, improves the nutritional level of fruit trees, increases the resistance of fruit trees, and inhibits the occurrence of diseases and insect pests.

[0049] Example 2:

[0050] A method for preparing compost for increasing the production of double-season black raspberries as described in Example 1 comprises the following steps:

[0051] Step 1, bacterial activation: Pseudomonas composting, Bacillus subtilis subsp. subtilis, Bacillus megaterium, Streptococcus thermophilus, Aspergillus oryzae, and Bacillus thuringiensis subsp. kenya are inoculated into a dedicated culture medium respectively, and cultured in a constant temperature shaking incubator;

[0052] Among them, Bacillus thuringiensis subspecies Kenya, Bacillus megaterium, Pseudomonas composting, and Bacillus subtilis were cultured at 28°C-30°C for 2-3 days, Aspergillus oryzae was cultured at 23°C-28°C for 4-7 days, and Streptococcus thermophilus was cultured at 42°C-45°C for 2-3 days, and all were subcultured for 2-3 cycles;

[0053] Bacteria acclimation: Add the activated bacterial fermentation liquid to the acclimation medium (containing 60%-80% drinking water, 5%-10% brown sugar, 3% milk powder, and 2% sodium chloride) in proportion, and culture at 28°C-32°C for 2-3 days. For thermophilic Streptococcus, culture at 40°C-45°C for 3-5 days. Subculture for 2-3 cycles.

[0054] Step 2, prepare mixed bacterial solution: mix the acclimated bacterial strains according to the proportion, culture at 30°C for 3 days, and dilute the fermentation solution with water at a ratio of 1:20 for later use;

[0055] Among them, the proportion of the domesticated strains is: 20%-25% of thermophilic Streptococcus liquid, 15%-20% of Aspergillus oryzae liquid, 20%-25% of composting Pseudomonas liquid, 10%-12% of Bacillus megaterium liquid, 10%-12% of Bacillus thuringiensis subspecies Kenya, and 15%-16% of Bacillus subtilis liquid.

[0056] Step 3, material processing: 25%-30% wheat straw, 20%-40% rice straw, and 5%-20% fallen leaves and weeds are crushed and mixed evenly;

[0057] Step 4: Layered composting: Stack in 5 layers, with odd-numbered layers consisting of fallen leaves and weeds, and even-numbered layers consisting of poultry manure and livestock manure. Spray the mixed bacterial solution between each layer, and cover the top with a 1-2 cm layer of soil and plastic film. Ferment for 90-100 days in winter and 60-75 days in summer, turning the pile at least 3 times during this period, and complete fermentation when the temperature drops below 38°C.

[0058] Furthermore, the dedicated culture medium comprises:

[0059] Compost Pseudomonas culture medium: tryptone 15.0 g / L, soytone 5.0 g / L, sodium chloride 5.0 g / L;

[0060] Streptococcus thermophilus culture medium: casein peptone 20 g / L, yeast extract powder 5.0 g / L, glucose 5 g / L, sucrose 5 g / L, lactose 5 g / L, Tween 80 1.0 mL / L, sodium chloride 4.0 g / L, sodium acetate 1.5 g / L, ascorbic acid 0.5 g / L;

[0061] Aspergillus oryzae and Bacillus subtilis culture medium: 1.0 L of 20% potato extract, 20.0 g / L of glucose;

[0062] Bacillus megaterium and Bacillus thuringiensis culture medium: peptone 5.0 g / L, beef extract 3.0 g / L, sodium chloride 5.0 g / L.

[0063] Furthermore, the acclimation culture medium is: 60%-80% drinking water, 5%-10% brown sugar, 3% milk powder, and 2% sodium chloride.

[0064] Furthermore, in step 4, the frequency of turning the compost is: once every 20-25 days in winter and once every 15-20 days in summer.

[0065] Furthermore, the number of viable bacteria in the bacterial fermentation liquid after acclimation is ≥1×10*cfu / ml.

[0066] Example 3:

[0067] The invention discloses an application of compost, which is used for the budding period, flowering period, fruit development period, primary stem rapid growth period and primary stem late development period of double-season black raspberry, with a fertilizer application rate of 1 ton per mu.

[0068] Example 4:

[0069] A compost for increasing the yield of double-season black raspberries is composed of the following raw materials in parts by weight: 30% wheat straw, 30% rice straw, 10% fallen leaves and weeds, 5% chicken manure, 5% goose manure, 5% sheep manure, 10% cow manure, and 5% bacterial liquid.

[0070] The bacterial liquid is composed of the following ingredients: 60% drinking water, 10% brown sugar, 3% milk powder, 25% bacterial fermentation liquid, and 2% sodium chloride;

[0071] The bacterial fermentation liquid consists of the following bacterial species: 5% thermophilic streptococcus liquid, 5% aspergillus oryzae liquid, 5% composting pseudomonas liquid, 3% bacillus megaterium liquid, 3% bacillus thuringiensis subspecies kenya, and 4% bacillus subtilis liquid.

[0072] The bacterial culture medium is formulated as follows: Pseudomonas composting: 15.0 g tryptone, 5.0 g soytone, 5.0 g sodium chloride, 1.0 L distilled water. Streptococcus thermophilus: 20 g casein peptone, 5.0 g yeast extract, 5 g glucose, 5 g sucrose, 5 g lactose, 1.0 mL Tween 80, 4.0 g sodium chloride, 1.5 g sodium acetate, 0.5 g ascorbic acid, distilled water to 1.0 L. Aspergillus oryzae and Bacillus subtilis subsp. subtilis: 1.0 L 20% potato extract, 20.0 g glucose. Bacillus megaterium and Bacillus thuringiensis subsp. kenya: 5.0 g peptone, 3.0 g beef extract, 5.0 g sodium chloride.

[0073] The method for preparing compost for increasing the production of double-season black raspberries comprises the following steps:

[0074] (1) Activation of bacterial strains: Pseudomonas composting, Bacillus subtilis subsp. subtilis, Bacillus megaterium liquid, Streptococcus thermophilus, Aspergillus oryzae, and Bacillus thuringiensis kenya subsp. are cultured using special culture media. The prepared culture media are poured into a triangular flask, and the freeze-dried powder of the bacterial strains is picked into the culture media using an inoculation needle. The culture is placed in a constant temperature shaking incubator for culture at a speed of 50 rpm. The culture temperature of Bacillus thuringiensis kenya subsp. subtilis, Bacillus megaterium, Pseudomonas composting, and Bacillus subtilis liquid are all 30°C and the culture time is 2 days. The culture temperature of Aspergillus oryzae is 28°C and the culture time is 4 days. The culture temperature of Streptococcus thermophilus is 42°C and the culture time is 3 days. The above bacterial strains are all cultured for 2 cycles and set aside.

[0075] (2) Acclimation of the Bacteria: Prepare an acclimation culture medium. Add the activated fermentation broth to the following acclimation medium in fixed proportions: drinking water 60%, brown sugar 10%, milk powder 3%, fermentation broth 25%, sodium chloride 2%. The culture temperature for Bacillus thuringiensis subsp. kenya, Bacillus megaterium, Pseudomonas composting, Bacillus subtilis, and Aspergillus oryzae is 32°C for 2 days, and the culture temperature for Streptococcus thermophilus is 45°C for 3 days. All the above bacteria are cultured for 2 cycles and then set aside.

[0076] (3) Preparation of mixed bacterial solution: The fermentation liquid of the six domesticated bacterial species was prepared in the following proportions: 20% of Streptococcus thermophilus liquid, 20% of Aspergillus oryzae liquid, 20% of Pseudomonas composting liquid, 12% of Bacillus megaterium liquid, 12% of Bacillus thuringiensis subsp. kenya liquid, and 16% of Bacillus subtilis liquid. The culture temperature was 30° C. for 3 days. After the culture, the fermentation liquid was mixed with water in a ratio of 1:20 and placed in a cool and ventilated place at an ambient temperature of 20° C.-30° C. for use.

[0077] (4) Material crushing: Use a crusher to crush rice straw, wheat straw, fallen leaves and weeds, mix them together in the following proportions: 40% wheat straw, 35% rice straw, and 25% fallen leaves and weeds, stir evenly, and set aside;

[0078] (5) Composting and fermentation: The compost is divided into 5 layers. The odd-numbered layers are made of straw, fallen leaves, grass and other materials, which are stacked evenly. The even-numbered layers are made of livestock and poultry manure. Mixed bacterial liquid is sprayed evenly between every two layers. After the 5 layers of materials are stacked, a 1cm-2cm layer of soil is covered on the top of the compost and covered with plastic film. Ferment for 100 days in winter and turn the compost once every 25 days. Ferment for 75 days in summer and turn the compost once every 15 days. It can be used after the fermentation temperature is below 38 degrees Celsius and no longer rises.

[0079] (6) Fertilization: Fertilization should be applied during the five stages of organic double-season black raspberry: budding stage, flowering stage, fruit development stage, primary stem rapid growth stage, and primary stem late development stage, with an application rate of 1 ton / mu.

[0080] Table 1 Mineral content of black raspberry leaves before and after fertilization (mg / kg)

[0081]

[0082] Example 5:

[0083] A compost for increasing the yield of double-season black raspberries, comprising the following raw materials in parts by weight: 25% wheat straw, 20% rice straw, 5% fallen leaves and weeds, 3% bacterial solution, 10% chicken manure, 5% goose manure, 17% sheep manure, 5% pig manure, and 10% cow manure;

[0084] The bacterial liquid is composed of the following ingredients: 80% drinking water, 5% brown sugar, 3% milk powder, 10% bacterial fermentation liquid, and 2% sodium chloride;

[0085] The bacterial fermentation liquid consists of the following bacterial species: 2% of thermophilic streptococcus liquid, 2% of aspergillus oryzae liquid, 3% of composting pseudomonas liquid, 1% of bacillus megaterium liquid, 1% of bacillus thuringiensis subspecies kenya, and 1% of bacillus subtilis liquid.

[0086] The bacterial culture medium is formulated as follows: Pseudomonas composting: 15.0 g tryptone, 5.0 g soytone, 5.0 g sodium chloride, 1.0 L distilled water. Streptococcus thermophilus: 20 g casein peptone, 5.0 g yeast extract, 5 g glucose, 5 g sucrose, 5 g lactose, 1.0 mL Tween 80, 4.0 g sodium chloride, 1.5 g sodium acetate, 0.5 g ascorbic acid, and distilled water to 1.0 L. Aspergillus oryzae and Bacillus subtilis subsp. subtilis: 1.0 L 20% potato extract, 20.0 g glucose. Bacillus megaterium and Bacillus thuringiensis subsp. kenya: 5.0 g peptone, 3.0 g beef extract, and 5.0 g sodium chloride.

[0087] The method for preparing compost for increasing the production of double-season black raspberries comprises the following steps:

[0088] (1) Activation of bacterial strains: Pseudomonas composting, Bacillus subtilis subsp. subtilis, Bacillus megaterium liquid, Streptococcus thermophilus, Aspergillus oryzae, and Bacillus thuringiensis kenya subsp. are cultured using special culture media. The prepared culture media are poured into a triangular flask, and the freeze-dried powder of the bacterial strains is picked into the culture media using an inoculation needle. The culture is placed in a constant temperature shaking incubator for culture at a speed of 80 rpm. The culture temperature of Bacillus thuringiensis kenya subsp. kenya subsp., Bacillus megaterium, Pseudomonas composting, and Bacillus subtilis liquid are all 28°C for 3 days, the culture temperature of Aspergillus oryzae is 23°C for 7 days, and the culture temperature of Streptococcus thermophilus is 45°C for 2 days. The above bacterial strains are all cultured for 3 cycles and set aside;

[0089] (2) Acclimation of the Bacteria: Prepare an acclimation culture medium. Add the activated fermentation broth to the following acclimation medium in fixed proportions: 80% drinking water, 5% brown sugar, 3% milk powder, 10% fermentation broth, and 2% sodium chloride. The culture temperature for Bacillus thuringiensis subsp. kenya, Bacillus megaterium, Pseudomonas composting, Bacillus subtilis, and Aspergillus oryzae is 28°C for 3 days, and the culture temperature for Streptococcus thermophilus is 40°C for 5 days. All the above strains are cultured for 3 cycles and then set aside.

[0090] (3) Preparation of mixed bacterial solution: The fermentation liquid of the six domesticated bacterial species was prepared in the following proportions: 25% of thermophilic Streptococcus liquid, 15% of Aspergillus oryzae liquid, 25% of composting Pseudomonas liquid, 10% of Bacillus megaterium liquid, 10% of Bacillus thuringiensis subsp. kenya liquid, and 15% of Bacillus subtilis liquid. The culture temperature was 30° C. for 3 days. After the culture, the fermentation liquid was mixed with water in a ratio of 1:20 and placed in a cool and ventilated place at an ambient temperature of 20° C.-30° C. for use.

[0091] (4) Material crushing: Use a crusher to crush rice straw, wheat straw, fallen leaves and weeds, mix them together in the following proportions: 25% wheat straw, 20% rice straw, and 5% fallen leaves and weeds, stir well, and set aside;

[0092] (5) Composting and fermentation: The compost is divided into 5 layers. The odd-numbered layers are made of straw, fallen leaves, grass and other materials, which are evenly stacked. The even-numbered layers are made of livestock and poultry manure. The mixed bacterial solution is sprayed evenly between every two layers. After the 5 layers of materials are stacked, a 1cm-2cm layer of soil is covered on the top of the compost and covered with plastic film. The compost is fermented for 90 days in winter and turned over once every 30 days. The compost is fermented for 60 days in summer and turned over once every 20 days. It can be used when the fermentation temperature is below 38 degrees Celsius and no longer rises.

[0093] (6) Fertilization: Fertilization should be applied during the five stages of organic double-season black raspberry: budding stage, flowering stage, fruit development stage, primary stem rapid growth stage, and primary stem late development stage, with an application rate of 1 ton / mu.

[0094] Example 6:

[0095] A compost for increasing the yield of double-season black raspberries is composed of the following raw materials in parts by weight: 25% wheat straw, 25% rice straw, 20% fallen leaves and weeds, 5% chicken manure, 20% sheep manure, and 5% bacterial liquid.

[0096] The bacterial liquid is composed of the following ingredients: 70% drinking water, 5% brown sugar, 10% milk powder, 10% bacterial fermentation liquid, and 5% sodium chloride;

[0097] The bacterial fermentation liquid consists of the following bacterial species: 2% of thermophilic streptococcus liquid, 2% of aspergillus oryzae liquid, 3% of composting pseudomonas liquid, 1% of bacillus megaterium liquid, 1% of bacillus thuringiensis subspecies kenya, and 1% of bacillus subtilis liquid.

[0098] The bacterial culture medium is formulated as follows: Pseudomonas composting: 15.0 g tryptone, 5.0 g soytone, 5.0 g sodium chloride, 1.0 L distilled water. Streptococcus thermophilus: 20 g casein peptone, 5.0 g yeast extract, 5 g glucose, 5 g sucrose, 5 g lactose, 1.0 mL Tween 80, 4.0 g sodium chloride, 1.5 g sodium acetate, 0.5 g ascorbic acid, and distilled water to 1.0 L. Aspergillus oryzae and Bacillus subtilis subsp. subtilis: 1.0 L 20% potato extract, 20.0 g glucose. Bacillus megaterium and Bacillus thuringiensis subsp. kenya: 5.0 g peptone, 3.0 g beef extract, and 5.0 g sodium chloride.

[0099] The method for preparing compost for increasing the production of double-season black raspberries comprises the following steps:

[0100] (1) Activation of bacterial strains: Pseudomonas composting, Bacillus subtilis subsp. subtilis, Bacillus megaterium liquid, Streptococcus thermophilus, Aspergillus oryzae, and Bacillus thuringiensis kenya subsp. are cultured using special culture media. The prepared culture media are poured into a triangular flask, and the freeze-dried powder of the bacterial strains is picked into the culture media using an inoculation needle. The culture is placed in a constant temperature shaking incubator for culture at a speed of 40-100 rpm. The culture temperature of Bacillus thuringiensis kenya subsp. subtilis, Bacillus megaterium, Pseudomonas composting, and Bacillus subtilis liquid are all 30°C and the culture time is 3 days. The culture temperature of Aspergillus oryzae is 28°C and the culture time is 5 days. The culture temperature of Streptococcus thermophilus is 43°C and the culture time is 3 days. The above bacterial strains are all subcultured for 3 cycles and set aside.

[0101] (2) Acclimation of bacteria: Prepare an acclimation culture medium. Add the activated bacterial fermentation liquid to the following acclimation medium in fixed proportions: drinking water 70%, brown sugar 5%, milk powder 10%, bacterial fermentation liquid 10%, sodium chloride 5%. The culture temperature for Bacillus thuringiensis subsp. kenya, Bacillus megaterium, Pseudomonas composting, Bacillus subtilis, and Aspergillus oryzae is 30°C for 3 days, and the culture temperature for Streptococcus thermophilus is 43°C for 4 days. All the above bacteria are subcultured for 3 cycles and set aside.

[0102] (3) Preparation of mixed bacterial solution: The fermentation liquid of the six domesticated bacterial species was prepared in the following proportions: 20% of Streptococcus thermophilus liquid, 20% of Aspergillus oryzae liquid, 30% of Pseudomonas composting liquid, 10% of Bacillus megaterium liquid, 10% of Bacillus thuringiensis subsp. kenya liquid, and 10% of Bacillus subtilis liquid. The culture temperature was 30° C. for 3 days. After the culture, the fermentation liquid was mixed with water in a ratio of 1:20 and placed in a cool and ventilated place at an ambient temperature of 20° C.-30° C. for use.

[0103] (4) Material crushing: Use a crusher to crush rice straw, wheat straw, fallen leaves and weeds, mix them together in the following proportions: 25% wheat straw, 25% rice straw, and 20% fallen leaves and weeds, stir evenly, and set aside;

[0104] (5) Composting and fermentation: The compost is divided into 5 layers. The odd-numbered layers are materials such as straw, fallen leaves, and grass, which are stacked evenly. The even-numbered layers are livestock and poultry manure. The mixed bacterial solution is sprayed evenly between every two layers. After the 5 layers of materials are stacked, a 1cm-2cm layer of soil is covered on the top of the compost and covered with plastic film. Ferment for 100 days in winter and turn the compost once every 25 days. Ferment for 60 days in summer and turn the compost once every 15 days. It can be used when the fermentation temperature is below 38 degrees Celsius and no longer rises.

[0105] (6) Fertilization: Fertilization should be applied during the five stages of organic double-season black raspberry: budding stage, flowering stage, fruit development stage, primary stem rapid growth stage, and primary stem late development stage, with an application rate of 1 ton / mu.

[0106] Table 2 Mineral content of organic fertilizer after fermentation (mg / kg)

[0107]

[0108] The substance detection method is microwave digestion-inductively coupled plasma mass spectrometry

[0109] Weigh 0.2g-0.3g of sample into a 50mL small beaker, add 5mL of concentrated nitric acid, cover with a watch glass, and soak overnight for later use. Then add 10mL of concentrated nitric acid, place the beaker on a constant temperature electric hot plate, control the temperature to 60℃, hold for 30min, then raise the temperature to 80℃. When no yellow smoke comes out of the sample, the pre-digestion is complete. Pour the pre-digested sample into a microwave digestion tank, set the temperature control program to 120℃ for 5min, increase to 150℃ at 5℃ / min, hold for 10min, then increase to 190℃ at 5℃ / min, hold for 20min, wait until the instrument temperature drops below 60℃, and remove the digestion tank. Place the digested sample on an electric heating plate, raise the temperature to 135℃ to drive out the acid, and when the digestion solution is 1-2mL, transfer the digestion solution to a 25mL volumetric flask with ultrapure water several times and make up to volume for testing. The instrument parameters are as follows: RF power: 1550 W, cooling gas flow rate 14 L / min, auxiliary gas flow rate 0.8 L / min, nebulizing gas flow rate 1.0 L / min, helium flow rate 4.9 mL / min, peristaltic pump speed 40 r / min, nebulization chamber temperature 2.6 °C, sampling depth 5 mm.

[0110]

[0111] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

Claims

1. A compost for increasing the production of double-season black raspberries, characterized in that: It is composed of the following raw materials in percentage by weight: Wheat straw 25%-30%, rice straw 20%-30%, fallen leaves and weeds 5%-20%, poultry manure 5%-15%, livestock manure 15%-32%, bacterial solution 3%-5%; the sum of all ingredients is 100%; Wherein, the poultry manure includes 5%-10% chicken manure and 0-5% goose manure; The livestock manure includes 5%-17% sheep manure, 0-5% pig manure and 0-10% cow manure; The bacterial liquid comprises: water, brown sugar, milk powder, sodium chloride, and bacterial fermentation liquid; The bacterial fermentation liquid includes: thermophilic streptococcus liquid, aspergillus oryzae liquid, composting pseudomonas liquid, bacillus megaterium liquid, bacillus thuringiensis subspecies kenya, and bacillus subtilis liquid.

2. The compost according to claim 1, characterized in that The weight percentages of the bacterial species in the bacterial fermentation liquid are as follows: 20%-25% of thermophilic streptococcus liquid, 15%-20% of aspergillus oryzae liquid, 20%-25% of composting pseudomonas liquid, 10%-12% of bacillus megaterium liquid, 10%-12% of bacillus thuringiensis subspecies kenya, and 15%-16% of bacillus subtilis liquid; the total of all the components is 100%.

3. The compost according to claim 1, characterized in that The weight percentages of the components in the bacterial liquid are: 60%-80% water, 5%-10% brown sugar, 3%-10% milk powder, 2%-5% sodium chloride, and 10%-25% bacterial fermentation liquid; the total of all the components is 100%.

4. A method for preparing compost according to any one of claims 1 to 3, characterized in that: The following steps are involved: Step 1, bacterial activation: Pseudomonas composting, Bacillus subtilis subsp. subtilis, Bacillus megaterium, Streptococcus thermophilus, Aspergillus oryzae, and Bacillus thuringiensis subsp. kenya are inoculated into a dedicated culture medium respectively, and cultured in a constant temperature shaking incubator; Among them, Bacillus thuringiensis subspecies Kenya, Bacillus megaterium, Pseudomonas composting, and Bacillus subtilis were cultured at 28°C-30°C for 2-3 days, Aspergillus oryzae was cultured at 23°C-28°C for 4-7 days, and Streptococcus thermophilus was cultured at 42°C-45°C for 2-3 days, and all were subcultured for 2-3 cycles; Strain acclimation: Add the activated strain fermentation liquid to the acclimation medium in proportion, culture at 28℃-32℃ for 2-3 days, culture thermophilic Streptococcus at 40℃-45℃ for 3-5 days, and subculture for 2-3 cycles; Step 2, prepare mixed bacterial solution: mix the acclimated bacterial strains according to the proportion, culture at 30°C for 3 days, and dilute the fermentation solution with water at a ratio of 1:20 for later use; The proportions of the domesticated strains are as follows: 20%-25% of thermophilic Streptococcus liquid, 15%-20% of Aspergillus oryzae liquid, 20%-25% of composting Pseudomonas liquid, 10%-12% of Bacillus megaterium liquid, 10%-12% of Bacillus thuringiensis subspecies Kenya, and 15%-16% of Bacillus subtilis liquid; Step 3, material processing: 25%-30% wheat straw, 20%-40% rice straw, and 5%-20% fallen leaves and weeds are crushed and mixed evenly; Step 4: Layered composting: Stack in 5 layers, with odd-numbered layers consisting of fallen leaves and weeds, and even-numbered layers consisting of poultry manure and livestock manure. Spray the mixed bacterial solution between each layer, and cover the top with a 1-2 cm layer of soil and plastic film. Ferment for 90-100 days in winter and 60-75 days in summer, turning the pile at least 3 times during this period, and complete fermentation when the temperature drops below 38°C.

5. The preparation method according to claim 4, characterized in that The dedicated culture medium comprises: Compost Pseudomonas culture medium: tryptone 15.0 g / L, soytone 5.0 g / L, sodium chloride 5.0 g / L; Streptococcus thermophilus culture medium: casein peptone 20 g / L, yeast extract powder 5.0 g / L, glucose 5 g / L, sucrose 5 g / L, lactose 5 g / L, Tween 80 1.0 mL / L, sodium chloride 4.0 g / L, sodium acetate 1.5 g / L, ascorbic acid 0.5 g / L; Aspergillus oryzae and Bacillus subtilis culture medium: 1.0 L of 20% potato extract, 20.0 g / L of glucose; Bacillus megaterium and Bacillus thuringiensis culture medium: peptone 5.0 g / L, beef extract 3.0 g / L, sodium chloride 5.0 g / L.

6. The preparation method according to claim 4, characterized in that The acclimation culture medium is: drinking water 60%-80%, brown sugar 5%-10%, milk powder 3%, and sodium chloride 2%.

7. The preparation method according to claim 4, characterized in that In step 4, the frequency of turning the compost is: once every 20-25 days in winter and once every 15-20 days in summer.

8. The preparation method according to claim 7, characterized in that The number of viable bacteria in the fermentation liquid of the strain after acclimation is ≥1×10 9 cfu / ml.

9. An application of the compost according to any one of claims 1 to 3 for use in the budding, flowering, fruit development, primary stem rapid growth, and primary stem late development stages of double-season black raspberries, characterized in that: The amount of fertilizer applied is 1 ton / mu.

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