Temperature detection device and method applied to recovery in NK cell preparation process

Abstract

The invention discloses a temperature detection device and method applied to recovery in the NK cell preparation process, and relates to the technical field of NK cell preparation, the temperature detection device comprises a servo motor and a temperature detection assembly, the output end of the servo motor is connected with a shaking table, the temperature detection assembly is arranged on one side of a hole site, and the shaking table is connected with the shaking table. A probe is fixed to the bottom of the temperature sensor body, a sleeve plate sleeves the lower portion of the outer wall of the probe, a sleeve box is fixed to the upper portion of the outer wall of the probe, and a rotating ring is connected to the inner wall of a bearing. According to the temperature detection device and method applied to resuscitation in the NK cell preparation process, a water body with stable and lasting temperature is provided for the continuous heat absorption process of resuscitation of a freezing pipe, and when the water body flows, a transmission blade is pushed to enable a rotating ring to rotate through a bearing; therefore, the second detection end can perform temperature detection on different parts of the outer wall of the freezing pipe, and the second detection end can synchronously shake along with the freezing pipe and always keep stable temperature detection.

Description

Technical Field

[0001] The present invention relates to the technical field of NK cell preparation, and in particular to a temperature detection device and method for resuscitation during NK cell preparation. Background Art

[0002] NK cells, or natural killer cells, are an important type of immune cell and part of the innate immune system. They play a role in identifying and eliminating infected cells, tumor cells and certain abnormal cells in the human body without prior contact with antigens or activation of specific immune responses. The preparation process of NK cells usually involves several steps: collection, enrichment, amplification, cryopreservation and recovery. When NK cells are converted from a frozen storage state to a use state, they need to be thawed and recovered through a recovery step. Usually, the frozen reagent tube is placed in a 37-degree Celsius water bath and gently shaken to thaw it quickly. During the recovery process, the water bath temperature and the temperature of the frozen reagent tube need to be monitored in real time by a temperature detection device to strictly control the temperature during the recovery process.

[0003] During the existing NK cell recovery process, the frozen reagent tube needs to be gently shaken. If the detection end of the temperature detection device is inside the reagent tube, it is easy to damage the internal cells due to shaking. If the detection end is outside the reagent tube, it is difficult to detect the temperature of the shaking reagent tube in real time, resulting in discontinuous and inaccurate temperature detection.

[0004] Therefore, in view of this, the existing structure and defects are studied and improved, and a temperature detection device and method for recovery during the NK cell preparation process are proposed. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the present invention provides a temperature detection device and method for resuscitation during the preparation process of NK cells, which solves the problems raised in the above background technology.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: A temperature detection device used for resuscitation during the preparation process of NK cells, comprising a servo motor and a temperature detection component, the output end of the servo motor is connected to a shaker, and holes are equidistantly provided on the surface of the shaker, and the temperature detection component is arranged on one side of the hole, and the temperature detection component comprises a temperature sensor body, a probe, a first detection end, a sleeve, a second detection end, a sleeve, a bearing, a rotating ring and a rubber sleeve, the bottom of the temperature sensor body is fixed with a probe, and the bottom of the probe is provided with a first detection end, the lower part of the outer wall of the probe is sleeved with a sleeve, and the side of the sleeve is provided with a second detection end, the upper part of the outer wall of the probe is fixed with a sleeve, and the top and bottom of the inner wall of the sleeve are provided with bearings, the inner wall of the bearing is connected to a rotating ring, and the inner wall of the rotating ring is fixed with a rubber sleeve.

[0007] Furthermore, the vertical center axis of the rotating ring coincides with the vertical center axis of the hole, and a freezing tube is passed through the hole.

[0008] Furthermore, the temperature detection component also includes an annular groove, and the interior of the sleeve is provided with an annular groove.

[0009] Furthermore, the temperature detection component also includes a water inlet end, and the water inlet end is provided on one side of the outer wall of the sleeve.

[0010] Furthermore, the temperature detection component also includes a micro pump, and the end of the water inlet is connected to the micro pump.

[0011] Furthermore, the temperature detection component also includes a water inlet pipe, the end of the micro pump is connected to the water inlet pipe, and the end of the water inlet pipe is located on the side of the inner wall of the water bath.

[0012] Furthermore, the temperature detection component also includes a transmission blade, and the transmission blade is fixed to the middle part of the outer wall of the rotating ring.

[0013] Furthermore, the temperature detection component also includes a water outlet, and the outer wall of the box is provided with a water outlet on the opposite side of the water inlet.

[0014] Furthermore, the temperature detection component also includes a spiral water distribution pipe, the end of the water outlet is connected to the spiral water distribution pipe, and the outer wall of the spiral water distribution pipe is a microporous structure.

[0015] A temperature detection method for resuscitation during NK cell preparation is provided, which uses the above-mentioned temperature detection device for resuscitation during NK cell preparation. The temperature detection method for resuscitation during NK cell preparation comprises the following steps:

[0016] Step 1: Before resuscitation, the temperature sensor body uses the first detection end to collect the water temperature around the rotating ring. Because the temperature of the water bath is often higher at the sides than in the middle when the water bath heats its internal water, the micro pump and water inlet pipe are used to extract water from the sides of the tank. The relatively high-temperature water passes through the water inlet end, the annular groove, and the water outlet end in sequence, and is discharged from the micropores on the surface of the spiral water distribution pipe. In this way, the relatively high-temperature water at the sides of the tank is transferred to the periphery of the rotating ring to ensure uniform water temperature.

[0017] Step 2: The first detection end is synchronously detected with the temperature sensor on the inner side of the water bath. When the temperature of each area reaches the preset temperature, the frozen tube that needs to be revived is inserted into the hole and the inside of the rotating ring from top to bottom, and the rubber sleeve is used to strengthen the clamping firmness of the frozen tube. The servo motor rotates at a constant speed in both directions to drive the shaker to shake at a constant speed. The frozen tube is revived with the shaker in the water bath. At this time, the outer wall of the frozen tube is tightly attached to the curved surface of the sleeve plate, and the second detection end is used to monitor the temperature of the outer wall of the frozen tube in real time, and the second detection end shakes synchronously with the frozen tube;

[0018] Step 3: During the recovery process of the frozen tube, the micro pump continues to transport the problem on the inner side of the groove to the periphery of the rotating ring to ensure that the water temperature outside the frozen tube is moderate and within the temperature threshold required for recovery. At the same time, when the water flows along the inside of the annular groove, it will push the transmission blades to make the rotating ring rotate through the bearing, thereby carrying the frozen tube to rotate on the curved surface of the sleeve plate, so that the second detection end can detect the temperature of different parts of the outer wall of the frozen tube.

[0019] The present invention provides a temperature detection device and method for resuscitation during NK cell preparation, which has the following beneficial effects:

[0020] 1. The temperature detection device and method for resuscitation in the NK cell preparation process are applied. Since the temperature sensor of the water bath is usually set at the side, the temperature detection of the water at the side of the tank is more accurate. By using a micro pump to extract water with relatively accurate temperature at the side of the tank and make it reach the periphery of the freezing tube, a stable and long-lasting temperature of water is provided for the continuous heat absorption process of the freezing tube during resuscitation, so as to ensure that the resuscitation conditions of the freezing tube meet the standards and are more accurate. Secondly, when the water flows along the inside of the annular groove, it pushes the transmission blades to make the rotating ring rotate through the bearing, thereby carrying the freezing tube to rotate on the curved surface of the sleeve plate, so that the second detection end can detect the temperature of different parts of the outer wall of the freezing tube, and the second detection end can shake synchronously with the freezing tube and always maintain stable temperature detection.

[0021] 2. The temperature detection device and method for resuscitation during the NK cell preparation process, before resuscitation, the temperature sensor body uses the first detection end to collect the water temperature around the rotating ring. Since the temperature of the water bath is often higher at the side of the tank than in the middle when the water bath heats the water inside, the water in the side area of the tank is extracted through a micro pump and a water inlet pipe, so that the relatively high-temperature water passes through the water inlet end, the annular groove, and the water outlet end in turn and is discharged from the micropores on the surface of the spiral water distribution pipe. As a result, the relatively high-temperature water at the side of the tank is transmitted to the periphery of the rotating ring to ensure uniform water temperature, which helps to shorten the heating time required for uniform water temperature and allows the water bath to reach the preset temperature faster for water bath resuscitation operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the structure of the shaking table of the present invention when clamping the freezing tube;

[0023] Figure 2 This is a structural schematic diagram of the shaking table of the present invention when no freezing tube is clamped;

[0024] Figure 3 This is a schematic diagram of the structure of the box of the present invention before the freezing tube is clamped inside;

[0025] Figure 4 This is a schematic diagram of the structure of the box of the present invention after the freezing tube is clamped inside;

[0026] Figure 5 It is a schematic diagram of the internal structure of the box of the present invention.

[0027] In the figure: 1. Servo motor; 2. Shaking table; 3. Hole position; 4. Temperature detection component; 401. Temperature sensor body; 402. Probe; 403. First detection end; 404. Bushing; 405. Second detection end; 406. Bushing; 407. Bearing; 408. Rotating ring; 409. Rubber sleeve; 410. Annular groove; 411. Water inlet end; 412. Micro pump; 413. Water inlet pipe; 414. Transmission blade; 415. Water outlet end; 416. Spiral water distribution pipe; 5. Freezing tube. DETAILED DESCRIPTION

[0028] The following embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0029] like Figure 1-Figure 5As shown, the present invention provides a technical solution: a temperature detection device for resuscitation during NK cell preparation, comprising a servo motor 1 and a temperature detection component 4, wherein the output end of the servo motor 1 is connected to a shaker 2, and holes 3 are equidistantly opened on the surface of the shaker 2, and the temperature detection component 4 is arranged on one side of the hole 3, and the temperature detection component 4 includes a temperature sensor body 401, a probe 402, a first detection end 403, a sleeve plate 404, a second detection end 405, a sleeve box 406, a bearing 407, a rotating ring 408 and a rubber sleeve 409, a probe 402 is fixed to the bottom of the temperature sensor body 401, and a first detection end 403 is provided at the bottom of the probe 402, a sleeve 404 is provided on the lower part of the outer wall of the probe 402, and a second detection end 405 is provided on the side of the sleeve 404, a sleeve box 406 is fixed to the upper part of the outer wall of the probe 402, and bearings 407 are provided on the top and bottom of the inner wall of the sleeve box 406, a rotating ring 408 is connected to the inner wall of the bearing 407, and a rubber sleeve 409 is fixed to the inner wall of the rotating ring 408, and the rotating ring 408 is provided with a rubber sleeve 409. The vertical center axis of 8 coincides with the vertical center axis of the hole 3, and the hole 3 is penetrated by a freezing tube 5, the temperature detection component 4 also includes an annular groove 410, the interior of the sleeve 406 is provided with an annular groove 410, the temperature detection component 4 also includes a water inlet end 411, and the outer wall of the sleeve 406 is provided with a water inlet end 411. The temperature detection component 4 also includes a micro pump 412, and the end of the water inlet end 411 is connected to the micro pump 412. The temperature detection component 4 also includes a water inlet pipe 413, and the end of the micro pump 412 is connected to the inlet The water pipe 413 is provided, and the end of the water inlet pipe 413 is located at the side of the inner wall of the water bath. The temperature detection component 4 also includes a transmission blade 414. The transmission blade 414 is fixed to the middle of the outer wall of the rotating ring 408. The temperature detection component 4 also includes a water outlet 415. The outer wall of the sleeve 406 is provided with a water outlet 415 on the opposite side of the water inlet 411. The temperature detection component 4 also includes a spiral water distribution pipe 416. The end of the water outlet 415 is connected to the spiral water distribution pipe 416, and the outer wall of the spiral water distribution pipe 416 has a microporous structure.

[0030] The specific operation is as follows: Since the heating components of the water bath are closer to the sides of the water, the water is heated from the outside to the inside. As a result, the temperature of the water outside is more likely to be higher than that of the middle part of the water when the water is heated. In order to maintain the overall temperature of the water, it is necessary to extend the heating time of the water to ensure uniform water temperature.

[0031] Before resuscitation, the temperature sensor body 401 uses the first detection end 403 to collect the water temperature around the rotating ring 408. Since the temperature of the side of the tank is often higher than the temperature in the middle when the water bath is heating the water inside, the water in the side area of the tank is extracted through the micro pump 412 and the water inlet pipe 413, so that the relatively high-temperature water passes through the water inlet end 411, the annular groove 410, and the water outlet end 415 in sequence and is discharged from the micropores on the surface of the spiral water distribution pipe 416. In this way, the relatively high-temperature water at the side of the tank is transmitted to the surrounding area of the rotating ring 408 to ensure uniform water temperature, which is conducive to shortening the heating time required for uniform water temperature and allowing the water bath to reach the preset temperature more quickly for water bath resuscitation operation.

[0032] The first detection end 403 is synchronously detected with the temperature sensor on the inner side of the water bath. When the temperature of each area reaches the preset temperature, the freezing tube 5 that needs to be revived is inserted into the hole 3 and the rotating ring 408 from top to bottom, and the rubber sleeve 409 is used to strengthen the clamping strength of the freezing tube 5. The servo motor 1 rotates at a constant speed in both directions to drive the shaking table 2 to shake at a constant speed. The freezing tube 5 is revived along with the shaking table 2 in the water bath. At this time, the outer wall of the freezing tube 5 is tightly attached to the curved surface of the sleeve 404, and the second detection end 405 is used to monitor the outer wall temperature of the freezing tube 5 in real time, and the second detection end 405 is used to monitor the outer wall temperature of the freezing tube 5 in real time. The end 405 shakes synchronously with the freezing tube 5. During the recovery process of the freezing tube 5, the micro pump 412 still keeps sending the problem on the inner side of the groove to the periphery of the rotating ring 408 to ensure that the temperature of the water outside the freezing tube 5 is moderate and within the temperature threshold required for recovery. At the same time, when the water flows along the inside of the annular groove 410, it pushes the transmission blade 414 to make the rotating ring 408 rotate through the bearing 407, thereby carrying the freezing tube 5 to rotate on the curved surface of the sleeve 404, so that the second detection end 405 can detect the temperature of different parts of the outer wall of the freezing tube 5;

[0033] Based on the above description, since the temperature sensor of the water bath is usually set at the side, the temperature detection of the water at the side of the tank is more accurate. By using the micro pump 412 to extract water with relatively accurate temperature at the side of the tank to reach the periphery of the freezing tube 5, a stable and lasting temperature of water is provided for the freezing tube 5 to continue to absorb heat during its recovery, so as to ensure that the recovery conditions of the freezing tube 5 meet the standards and are more accurate. Secondly, when the water flows along the annular groove 410, it will push the transmission blade 414 to make the rotating ring 408 rotate through the bearing 407, thereby carrying the freezing tube 5 to rotate on the curved surface of the sleeve plate 404, so that the second detection end 405 can perform temperature detection on different parts of the outer wall of the freezing tube 5, and the second detection end 405 can shake synchronously with the freezing tube 5 and always maintain stable temperature detection.

[0034] like Figure 1-Figure 5As shown, a temperature detection method for resuscitation during NK cell preparation is applied with the above-mentioned temperature detection device for resuscitation during NK cell preparation. The temperature detection method for resuscitation during NK cell preparation includes the following steps:

[0035] Step 1: Before resuscitation, the temperature sensor body 401 uses the first detection end 403 to collect the water temperature around the rotating ring 408. Since the temperature of the water bath is often higher at the sides than in the middle when the water bath heats its internal water, the water at the sides of the bath is extracted through the micro pump 412 and the water inlet pipe 413. The relatively high-temperature water passes through the water inlet end 411, the annular groove 410, and the water outlet end 415 in sequence and is discharged from the micropores on the surface of the spiral water distribution pipe 416. In this way, the relatively high-temperature water at the sides of the bath is transmitted to the surrounding area of the rotating ring 408 to ensure uniform water temperature.

[0036] Step 2: The first detection end 403 is synchronously detected with the temperature sensor on the inner side of the water bath. When the temperature of each area reaches the preset temperature, the freezing tube 5 that needs to be revived is inserted into the hole 3 and the rotating ring 408 from top to bottom, and the rubber sleeve 409 is used to strengthen the clamping strength of the freezing tube 5. The servo motor 1 rotates at a constant speed in both directions to drive the shaking table 2 to shake at a constant speed. The freezing tube 5 is revived along with the shaking table 2 in the water bath. At this time, the outer wall of the freezing tube 5 is tightly attached to the curved surface of the sleeve plate 404, and the second detection end 405 is used to monitor the outer wall temperature of the freezing tube 5 in real time, and the second detection end 405 shakes synchronously with the freezing tube 5;

[0037] Step 3: During the resuscitation of the freezing tube 5, the micro pump 412 continues to transport the problem on the inner side of the groove to the periphery of the rotating ring 408 to ensure that the water temperature outside the freezing tube 5 is moderate and within the temperature threshold required for resuscitation. At the same time, when the water flows along the annular groove 410, it will push the transmission blades 414 to make the rotating ring 408 rotate through the bearing 407, thereby carrying the freezing tube 5 to rotate on the curved surface of the sleeve plate 404, so that the second detection end 405 can perform temperature detection on different parts of the outer wall of the freezing tube 5.

[0038] The embodiments of the present invention are presented for purposes of illustration and description and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those skilled in the art. The embodiments are chosen and described in order to better illustrate the principles of the invention and its practical application and to enable those skilled in the art to understand the invention and design various embodiments with various modifications as suited for specific applications.

Claims

1. A temperature detection device for resuscitation during NK cell preparation, comprising a servo motor (1) and a temperature detection component (4), characterized in that: The output end of the servo motor (1) is connected to a shaking table (2), and holes (3) are equidistantly provided on the surface of the shaking table (2). The temperature detection component (4) is arranged on one side of the hole (3). The temperature detection component (4) comprises a temperature sensor body (401), a probe (402), a first detection end (403), a sleeve (404), a second detection end (405), a sleeve (406), a bearing (407), a rotating ring (408) and a rubber sleeve (409). The bottom of the temperature sensor body (401) is fixed with a probe. The probe (402) is provided with a first detection end (403) at the bottom of the probe (402), a sleeve (404) is provided on the lower part of the outer wall of the probe (402), and a second detection end (405) is provided on the side of the sleeve (404), a sleeve box (406) is fixed on the upper part of the outer wall of the probe (402), and bearings (407) are provided on the top and bottom of the inner wall of the sleeve box (406), the inner wall of the bearing (407) is connected to a rotating ring (408), and a rubber sleeve (409) is fixed on the inner wall of the rotating ring (408).

2. A temperature detection device for resuscitation during NK cell preparation according to claim 1, characterized in that: The vertical center axis of the rotating ring (408) coincides with the vertical center axis of the hole (3), and a freezing tube (5) is passed through the hole (3).

3. The temperature detection device for resuscitation during NK cell preparation according to claim 1, characterized in that: The temperature detection component (4) further comprises an annular groove (410), and the interior of the sleeve (406) is provided with an annular groove (410).

4. The temperature detection device for resuscitation during NK cell preparation according to claim 1, characterized in that: The temperature detection component (4) further comprises a water inlet end (411), and the water inlet end (411) is provided on one side of the outer wall of the sleeve box (406).

5. The temperature detection device for resuscitation during NK cell preparation according to claim 4, characterized in that: The temperature detection component (4) further comprises a micro pump (412), and the end of the water inlet end (411) is connected to the micro pump (412).

6. The temperature detection device for resuscitation during NK cell preparation according to claim 5, characterized in that: The temperature detection component (4) further comprises a water inlet pipe (413), the end of the micro pump (412) is connected to the water inlet pipe (413), and the end of the water inlet pipe (413) is located at the side of the inner wall of the water bath.

7. The temperature detection device for resuscitation during NK cell preparation according to claim 1, characterized in that: The temperature detection component (4) further includes a transmission blade (414), and the transmission blade (414) is fixed to the middle portion of the outer wall of the rotating ring (408).

8. The temperature detection device for resuscitation during NK cell preparation according to claim 4, characterized in that: The temperature detection component (4) further comprises a water outlet (415), and the outer wall of the casing (406) is provided with a water outlet (415) on the side opposite to the water inlet (411).

9. A temperature detection device for resuscitation during NK cell preparation according to claim 8, characterized in that: The temperature detection component (4) further comprises a spiral water distribution pipe (416), the end of the water outlet (415) is connected to the spiral water distribution pipe (416), and the outer wall of the spiral water distribution pipe (416) has a microporous structure.

10. A temperature detection method for resuscitation during NK cell preparation, comprising the temperature detection device for resuscitation during NK cell preparation according to any one of claims 1 to 9, characterized in that: The temperature detection method for recovery during NK cell preparation comprises the following steps: Step 1: Before resuscitation, the temperature sensor body (401) uses the first detection end (403) to collect the water temperature around the rotating ring (408). Since the temperature of the water bath is often higher at the edge of the tank than in the middle when the water bath heats the water inside, the water in the edge area of the tank is extracted through the micro pump (412) and the water inlet pipe (413), so that the relatively high-temperature water passes through the water inlet end (411), the annular groove (410), and the water outlet end (415) in sequence and is discharged from the micropores on the surface of the spiral water distribution pipe (416). In this way, the relatively high-temperature water at the edge of the tank is transmitted to the periphery of the rotating ring (408) to ensure uniform water temperature. Step 2: The first detection end (403) is synchronously detected with the temperature sensor on the inner side of the water bath. When the temperature of each area reaches the preset temperature, the freezing tube (5) that needs to be revived is inserted into the hole (3) and the rotating ring (408) from top to bottom, and the rubber sleeve (409) is used to strengthen the clamping strength of the freezing tube (5). The servo motor (1) rotates at a constant speed in both directions to drive the shaking table (2) to shake at a constant speed. The freezing tube (5) is revived along with the shaking table (2) in the water bath. At this time, the outer wall of the freezing tube (5) is tightly attached to the arc surface of the sleeve plate (404), and the second detection end (405) is used to monitor the outer wall temperature of the freezing tube (5) in real time, and the second detection end (405) shakes synchronously with the freezing tube (5); Step 3: During the recovery process of the freezing tube (5), the micro pump (412) still keeps transporting the problem on the inner side of the groove to the periphery of the rotating ring (408) to ensure that the temperature of the water outside the freezing tube (5) is moderate and within the temperature threshold required for recovery. At the same time, when the water flows along the inside of the annular groove (410), it will push the transmission blade (414) to make the rotating ring (408) rotate through the bearing (407), thereby carrying the freezing tube (5) to rotate on the curved surface of the sleeve (404), so that the second detection end (405) can detect the temperature of different parts of the outer wall of the freezing tube (5).

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