Stem cell culture device and method

Abstract

The invention discloses a stem cell culture device and method, and belongs to the technical field of stem cell culture. The stem cell culture device comprises a culture box and further comprises a partition plate fixedly connected in the culture box, the culture box is divided into an upper cavity and a lower cavity through the partition plate, an inlet communicated with the upper cavity is formed in the top of the culture box, and a sealing cover is installed at the inlet; the supporting plate is arranged at the top of the partition plate, a culture dish is arranged at the top of the supporting plate, and a driving part for driving the supporting plate to shake is arranged on the partition plate; according to the stem cell culture device, a culture dish at the upper end of the support plate can be shaken by simulating manual work, so that nutrient substances in the culture dish are uniformly mixed and contacted with stem cells, and the culture effect of the stem cells is effectively improved under the condition that the stem cells are not easily damaged.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture, and in particular to a stem cell culture device and method. Background Art

[0002] Stem cells are multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. Depending on their developmental stage, they are categorized as embryonic stem cells and adult stem cells. Stem cells are immature, underdifferentiated cells with the potential to regenerate various tissues, organs, and the human body. They are known in the medical community as "universal cells." Therefore, the cultivation of stem cells is extremely important.

[0003] Currently, most existing stem cell culture devices are static culture devices, which prevents the stem cells in the culture dish from evenly contacting nutrients and oxygen, resulting in slow growth and reproduction of stem cells and reduced stem cell culture efficiency. If the nutrients are stirred to improve their uniformity, the stem cells are easily damaged, which will also affect the culture effect. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem in the prior art that the growth and reproduction of stem cells are slow, which reduces the culture efficiency of stem cells, and to propose a stem cell culture device and method.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A stem cell culture device includes an incubator, and also includes: a partition fixedly connected to the incubator, wherein the incubator is divided into an upper cavity and a lower cavity by the partition, and an inlet connected to the upper cavity is provided at the top of the incubator, and a sealing cover is installed at the inlet; a support plate arranged on the top of the partition, wherein a culture dish is provided on the top of the support plate, and the partition is provided with a driving unit for driving the support plate to shake.

[0007] In order to facilitate continuous shaking of the culture dish, preferably, the driving part includes a first ring gear rotatably connected to the partition, an active magnet is fixedly connected to the first ring gear, and the lower end of the support plate is fixedly connected to a plurality of driven magnets distributed in a ring, wherein the active magnet absorbs one of the driven magnets, an elastic column is fixedly installed on the bottom of the support plate, the lower end of the elastic column is fixedly connected to the partition, a driving motor is fixedly installed on the partition, and the output shaft of the driving motor is fixedly installed with a driving gear that drives the first ring gear to rotate.

[0008] In order to facilitate uniform heating of the culture environment, preferably, the circumferential outer wall of the first ring gear is fixedly connected to a support plate, a heating column is rotatably connected to the support plate, a support rod is fixedly connected to the heating column, and a linkage part for driving the heating column to rotate is provided on the support plate.

[0009] In order to enable the heating column to rotate, preferably, the linkage part includes a passive gear fixedly mounted on the bottom of the heating column, and the partition is fixedly connected to a second ring gear meshing with the passive gear.

[0010] In order to facilitate the improvement of the oxygen content of nutrients, preferably, the lower end of the culture dish is provided with a plurality of sockets, the inner wall of the upper end of the culture dish is provided with a plurality of groups of exhaust holes distributed circumferentially, the exhaust holes are connected to the sockets, the support plate is fixedly connected with a column inserted into the sockets, and the support plate is provided with an air supply part for supplying air to the sockets.

[0011] In order to automatically supply air to the exhaust holes, preferably, the air supply part includes a plurality of elastic air bags installed on the upper edge of the support plate, and the plurality of elastic air bags are fixedly connected with an intake pipe and an exhaust pipe, wherein a one-way valve is fixedly installed in the intake pipe and the exhaust pipe, and the output end of the exhaust pipe extends to the top of the column, and an annular plate is fixedly connected to the upper cavity through a bracket, and the annular plate is located above the plurality of elastic air bags.

[0012] In order to further improve the heat preservation effect of the incubator, preferably, a heating wire is fixedly installed on the inner bottom of the lower cavity, and the shape of the heating wire is annular.

[0013] In order to humidify the air sucked in by the intake pipe, preferably, the end of the intake pipe extends to the inner top of the lower cavity, the top of the incubator is provided with an air inlet extending into the lower cavity, the sealing cover is provided with an air outlet, and a one-way valve is installed in the air inlet and the air outlet.

[0014] In order to prevent dust from contaminating the air entering the incubator, a vertical pipe is fixedly connected to the bottom of the incubator, the air inlet end of the air inlet hole is located at the upper end of the vertical pipe, and a filter element is installed in the lower end of the vertical pipe.

[0015] A stem cell culture method, comprising the following steps:

[0016] Step 1: Place the stem cells to be cultured and nutrients in a culture dish and allow the incubator to reach the preset culture environment;

[0017] Step 2: Make the support plate simulate manual shaking of the culture dish on top of it;

[0018] Step 3: Make the multiple heating columns and support rods revolve around the culture dish;

[0019] Step 4: The heating column drives the support rod to revolve around the axis of the heating column;

[0020] Step 5: Allow the nutrients in the culture dish to be blown by air.

[0021] Compared with the prior art, the present invention provides a stem cell culture device having the following

[0022] Beneficial effects:

[0023] 1. This stem cell culture device, by allowing the active magnet to pass under multiple driven magnets in sequence, causes the support plate to change its tilt direction in different directions, making the support plate simulate artificial shaking of the culture dish on its upper end, thereby allowing the nutrients in the culture dish to mix and contact with the stem cells evenly, effectively improving the stem cell culture effect without easily damaging the stem cells.

[0024] 2. This stem cell culture device uses a support plate to drive multiple heating columns to revolve around the culture dish. The heating columns will drive the support rods to revolve synchronously, thereby evenly maintaining the temperature in the incubator and achieving better culture effects.

[0025] 3. This stem cell culture device uses a heating column to drive the passive gear to roll along the second ring gear, and the passive gear drives the heating column to rotate. As a result, the heating column can also drive the support rod to revolve around the axis of the heating column, further improving the uniformity of the heated culture environment and achieving better culture effects.

[0026] 4. This stem cell culture device uses a support plate to drive multiple elastic airbags to tilt synchronously, so that the elastic airbag at the highest point will press against the bottom of the annular plate. The elastic airbag will transport air to the jack through the exhaust pipe. The air will eventually blow into the culture dish from the exhaust hole, ensuring the oxygen content in the nutrients and further improving the effect of stem cell culture.

[0027] 5. The stem cell culture device draws air into the incubator through an air intake pipe, and the incubator draws air through the air inlet. The air passes through the pure water and is drawn into the air intake pipe, where it becomes moist. The exhaust hole can then spray air with a high humidity into the nutrients, ensuring the humidity of the nutrients. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the axonometric structure of a stem cell culture device proposed in the present invention;

[0029] Figure 2 This is a schematic diagram of a partial axonometric structure of a stem cell culture device proposed in the present invention;

[0030] Figure 3 A schematic diagram of a partial axonometric cross-section of a stem cell culture device proposed in the present invention Figure 1 ;

[0031] Figure 4 A schematic diagram of a partial axonometric cross-section of a stem cell culture device proposed in the present invention Figure 2 ;

[0032] Figure 5 This is a schematic diagram of the isometric structure of a support plate of a stem cell culture device proposed in the present invention;

[0033] Figure 6 This is a schematic diagram of the axonometric structure of the first ring gear of a stem cell culture device proposed in the present invention;

[0034] Figure 7 This is a schematic diagram of the cutaway axonometric structure of a culture dish of a stem cell culture device proposed in the present invention;

[0035] Figure 8 This is a schematic diagram of the axonometric structure of a ring plate of a stem cell culture device proposed in the present invention.

[0036] In the figure: 1. incubator; 2. upper cavity; 3. lower cavity; 4. partition; 5. inlet; 6. culture dish; 7. elastic column; 8. support plate; 9. first ring gear; 10. active magnet; 11. driven magnet; 12. drive motor; 13. active gear; 14. support plate; 15. heating column; 16. driven gear; 17. second ring gear; 18. jack; 19. exhaust hole; 20. column; 21. bracket; 22. ring plate; 23. elastic airbag; 24. suction pipe; 25. support rod; 26. heating wire; 27. vertical pipe; 28. air inlet; 29. filter element; 30. sealing cover; 31. transparent window; 32. exhaust pipe. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0038] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0039] Example 1:

[0040] Reference Figures 1-8A stem cell culture device includes an incubator 1 with heating and heat preservation functions, and also includes: a partition 4, fixedly connected to the incubator 1, wherein the incubator 1 is divided into an upper cavity 2 and a lower cavity 3 by the partition 4, the upper cavity 2 is used for culturing stem cells, and an inlet 5 connected to the upper cavity 2 is provided on the top of the incubator 1, and a sealing cover 30 for sealing the inlet 5 is installed at the inlet 5, and the sealing cover 30 can be provided with air holes and a transparent window 31 as needed; a support plate 8 is arranged on the top of the partition 4, wherein a culture dish 6 for storing stem cells and nutrients is provided on the top of the support plate 8, and a driving part for driving the support plate 8 to shake is provided on the partition 4.

[0041] During use, the stem cells to be cultured and the nutrients are placed in the culture dish 6, and then the inlet 5 is sealed by the sealing cover 30. At this time, the incubator 1 is heated to reach the preset culture environment, and then the support plate 8 is driven to shake by the driving part. The support plate 8 will simulate the artificial shaking of the culture dish 6 on its upper end, thereby making the nutrients in the culture dish 6 and the stem cells evenly mixed and contacted, effectively improving the culture effect of the stem cells without easily damaging the stem cells.

[0042] Example 2:

[0043] Reference Figure 3-Figure 6 , which is basically the same as the first embodiment, furthermore, specifically discloses a specific implementation scheme of the driving part.

[0044] The above-mentioned driving part includes a first ring gear 9 rotatably connected to the partition 4, and an active magnet 10 is fixedly connected to the first ring gear 9. The lower end of the support plate 8 is fixedly connected to a plurality of driven magnets 11 distributed in a ring. The number of driven magnets 11 is 20-40, and the preferred number in this application is 30. The magnetic poles of the opposite surfaces of the active magnet 10 and the driven magnet 11 are opposite, wherein the active magnet 10 adsorbs one of the driven magnets 11, and an elastic column 7 is fixedly installed at the bottom of the support plate 8. The elastic column 7 can be a spring. The lower end of the elastic column 7 is fixedly connected to the partition 4, and a drive motor 12 is fixedly installed on the partition 4. The output shaft of the drive motor 12 is fixedly installed with a driving gear 13 that drives the first ring gear 9 to rotate.

[0045] Specifically, during use, the stem cells to be cultured and the nutrients are placed in the culture dish 6, and then the sealing cover 30 is used to seal the inlet 5. At this time, the incubator 1 is heated to reach the preset culture environment, and then the drive motor 12 is started. The drive motor 12 drives the driving gear 13 to rotate, and the driving gear 13 drives the first ring gear 9 to rotate. The first ring gear 9 drives the driving magnet 10 to revolve around the axis of the first ring gear 9, so that the driving magnet 10 passes under the multiple driven magnets 11 in sequence. When the driving magnet 10 is directly under one of the driven magnets 11, the driven magnet 11 will approach the driving magnet 10 under the action of magnetic force, and the support plate 8 will also tilt toward the driving magnet 10. Therefore, when the active magnet 10 continues to revolve, the support plate 8 will change its tilt direction in different ways, so that the support plate 8 simulates artificial shaking of the culture dish 6 on its upper end, thereby evenly mixing and contacting the nutrients in the culture dish 6 with the stem cells, effectively improving the culture effect of the stem cells without easily damaging the stem cells.

[0046] Example 3:

[0047] Reference Figure 3-Figure 6 , which is basically the same as Example 2, further discloses a specific implementation plan for heating and keeping the environment warm.

[0048] The circumferential outer wall of the above-mentioned first ring gear 9 is fixedly connected with a support plate 14, and a heating column 15 is rotatably connected to the support plate 14. The heating column 15 is fixedly connected with a support rod 25. An electric heating wire is installed in the heating column 15. The support plate 14 is provided with a linkage part for driving the heating column 15 to rotate. The linkage part includes a passive gear 16 fixedly installed at the bottom of the heating column 15, and a second ring gear 17 meshing with the passive gear 16 is fixedly connected to the partition 4.

[0049] Specifically, during use, the stem cells to be cultured and the nutrients are placed in the culture dish 6, and then the sealing cover 30 is used to seal the entrance 5. At this time, the incubator 1 is heated to reach the preset culture environment, and then the driving part drives the support plate 8 to shake. The support plate 8 will simulate the artificial shaking of the culture dish 6 on its upper end, thereby making the nutrients in the culture dish 6 and the stem cells evenly mixed and contacted, effectively improving the culture effect of the stem cells without easily damaging the stem cells. When the first ring gear 9 rotates, the first ring gear 9 will also drive the multiple heating columns 15 to revolve around the culture dish 6 through the support plate 14, and the heating column 15 will drive the support rod 25 to revolve synchronously, so that the temperature in the incubator 1 can be evenly maintained, so that the culture effect is better. When the heating column 15 revolves, the heating column 15 will also drive the driven gear 16 to roll along the second ring gear 17, and the driven gear 16 will drive the heating column 15 to rotate, so that the heating column 15 can also drive the support rod 25 to revolve around the axis of the heating column 15, further improving the uniformity of the heated culture environment and achieving better culture effect.

[0050] Example 4:

[0051] Reference Figure 3-Figure 8 , which is basically the same as Example 3, and furthermore, a specific implementation plan for increasing the oxygen content of nutrients is specifically added.

[0052] The lower end of the above-mentioned culture dish 6 is provided with a plurality of sockets 18, and the inner wall of the upper end of the culture dish 6 is provided with a plurality of groups of exhaust holes 19 distributed circumferentially. The exhaust holes 19 are provided with 15 groups to 30 groups. The present application preferably provides 20 groups, and each group is provided with three exhaust holes 19. The exhaust holes 19 are connected to the sockets 18. A column 20 inserted into the sockets 18 is fixedly connected to the support plate 8, and an air supply part for supplying air to the sockets 18 is provided on the support plate 8; the air supply part includes a plurality of elastic air bags 23 installed on the upper end edge of the support plate 8, and the plurality of elastic air bags 23 are fixedly connected with an intake pipe 24 and an exhaust pipe 32, wherein a one-way valve is fixedly installed in the intake pipe 24 and the exhaust pipe 32, and the output end of the exhaust pipe 32 extends to the top of the column 20, and an annular plate 22 is fixedly connected to the upper cavity 2 through a bracket 21, and the annular plate 22 is located above the plurality of elastic air bags 23.

[0053] Specifically, when the support plate 8 is tilted, the support plate 8 will drive multiple elastic airbags 23 to tilt synchronously, so that the elastic airbag 23 at the highest point will press against the bottom of the annular plate 22, and the elastic airbag 23 will transport air to the jack 18 through the exhaust pipe 32. The air will eventually be blown into the culture dish 6 from the exhaust hole 19. At this time, the nutrients in the tilted state, that is, the nutrients that were not on the surface before, will be blown by the air in time, ensuring the oxygen content in the nutrients and further improving the effect of culturing stem cells. When the elastic airbag 23 is not squeezed, it will elastically reset and absorb air through the intake pipe 24.

[0054] Embodiment 5:

[0055] Reference Figure 2-Figure 4 , which is basically the same as Example 4, and further, a specific implementation plan for ensuring the moisture content of nutrients is specifically added.

[0056] A heating wire 26 is fixedly mounted on the inner bottom of the lower cavity 3 , and the heating wire 26 has a circular ring shape.

[0057] Specifically, during use, pure water is placed in the lower cavity 3 and then heated by the heating wire 26 , thereby improving the heat preservation effect of the incubator 1 .

[0058] The end of the above-mentioned suction pipe 24 extends to the inner top of the lower cavity 3. The top of the incubator 1 is provided with an air inlet 28 extending into the lower cavity 3, and the sealing cover 30 is provided with an air outlet. Both the air inlet 28 and the air outlet are installed with a one-way valve. The bottom of the incubator 1 is fixedly connected to a vertical pipe 27. The air inlet end of the air inlet 28 is located at the upper end of the vertical pipe 27. The lower end of the vertical pipe 27 is installed with a filter element 29 for filtering dust and harmful substances.

[0059] Specifically, when the air intake pipe 24 draws air, the air intake pipe 24 will draw air into the incubator 1, and the incubator 1 will draw air through the air inlet 28. The air will pass through the pure water and be drawn into the air intake pipe 24, and the air will be moistened. Then, the exhaust hole 19 can spray air with high humidity into the nutrients, ensuring the humidity of the nutrients. When the air passes through the vertical pipe 27, the filter element 29 in the vertical pipe 27 will filter the dust in the air, reducing the impact of dust on the stem cell culture process.

[0060] A stem cell culture method, comprising the following steps:

[0061] Step 1: Place the stem cells to be cultured and nutrients in the culture dish 6 and allow the incubator 1 to reach a preset culture environment;

[0062] Step 2: Make the support plate 8 simulate manual shaking of the culture dish 6 on top of it;

[0063] Step 3: Make the multiple heating columns 15 and the support rods 25 revolve around the culture dish 6;

[0064] Step 4: The heating column 15 drives the support rod 25 to revolve around the axis of the heating column 15;

[0065] Step 5: Allow the nutrients in the culture dish 6 to be blown by air.

[0066] When the present stem cell culture device is in use, the stem cells to be cultured and nutrients are placed in the culture dish 6, which is then sealed at the inlet 5 by the sealing cover 30. At this time, the incubator 1 is heated to reach a preset culture environment, and then the drive motor 12 is started. The drive motor 12 drives the driving gear 13 to rotate, which in turn drives the first ring gear 9 to rotate. The first ring gear 9 drives the driving magnet 10 to revolve around the axis of the first ring gear 9. The driving magnet 10 then passes under the multiple driven magnets 11 in sequence. When the driving magnet 10 is directly under one of the driven magnets 11, the driven magnet 11 approaches the driving magnet 10 under the action of the magnetic force, and the support plate 8 also tilts toward the driving magnet 10. As the active magnet 10 continues to revolve, the support plate 8 changes its tilt direction, simulating artificial shaking of the culture dish 6 on its upper end, thereby evenly mixing and contacting the nutrients in the culture dish 6 with the stem cells, effectively improving the stem cell culture effect without damaging the stem cells.

[0067] When the first ring gear 9 rotates, the first ring gear 9 will also drive multiple heating columns 15 to revolve around the culture dish 6 through the support plate 14, and the heating columns 15 will drive the support rods 25 to revolve synchronously, so that the temperature in the incubator 1 can be evenly maintained, so that the culture effect is better. When the heating columns 15 revolve, the heating columns 15 will also drive the passive gear 16 to roll along the second ring gear 17, and the passive gear 16 will drive the heating columns 15 to rotate. Then, the heating columns 15 can also drive the support rods 25 to revolve around the axis of the heating columns 15, further improving the uniformity of the heated culture environment and achieving better culture effects.

[0068] When the support plate 8 is tilted, the support plate 8 will drive the multiple elastic airbags 23 to tilt synchronously, so that the elastic airbag 23 at the highest point will press against the bottom of the annular plate 22, and the elastic airbag 23 will transport air to the jack 18 through the exhaust pipe 32. The air will eventually be blown into the culture dish 6 from the exhaust hole 19. At this time, the nutrients in the tilted state, that is, the nutrients that were not on the surface before, will be blown by the air in time, ensuring the oxygen content in the nutrients and further improving the effect of culturing stem cells. When the elastic airbag 23 is not squeezed, it will elastically reset and absorb air through the suction pipe 24.

[0069] During use, pure water is placed in the lower cavity 3, and then the water is heated by the heating wire 26, thereby improving the insulation effect of the incubator 1. When the air is sucked in by the suction pipe 24, the suction pipe 24 will suck air into the incubator 1, and the incubator 1 will suck air through the air inlet 28. The air will pass through the pure water and be sucked in by the suction pipe 24. The air will be moistened, so the exhaust hole 19 can spray air with high humidity into the nutrients, ensuring the humidity of the nutrients. When the air passes through the vertical pipe 27, the filter element 29 in the vertical pipe 27 will filter the dust in the air, reducing the impact of dust on the stem cell culture process.

[0070] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A stem cell culture device, comprising an incubator (1), characterized in that: Also includes: A partition (4) is fixedly connected in the incubator (1), The incubator (1) is divided into an upper cavity (2) and a lower cavity (3) by a partition (4); an inlet (5) communicating with the upper cavity (2) is provided at the top of the incubator (1); and a sealing cover (30) is installed at the inlet (5); A support plate (8) is provided on top of the partition (4), A culture dish (6) is provided on the top of the support plate (8), and a driving unit for driving the support plate (8) to shake is provided on the partition plate (4).

2. A stem cell culture device according to claim 1, characterized in that: The driving part comprises a first ring gear (9) rotatably connected to the partition (4), a driving magnet (10) being fixedly connected to the first ring gear (9), and a plurality of driven magnets (11) distributed in an annular manner being fixedly connected to the lower end of the support plate (8). The active magnet (10) absorbs one of the driven magnets (11), an elastic column (7) is fixedly mounted on the bottom of the support plate (8), the lower end of the elastic column (7) is fixedly connected to the partition (4), a driving motor (12) is fixedly mounted on the partition (4), and an output shaft of the driving motor (12) is fixedly mounted with a driving gear (13) that drives the first ring gear (9) to rotate.

3. A stem cell culture device according to claim 2, characterized in that: A support plate (14) is fixedly connected to the circumferential outer wall of the first ring gear (9), a heating column (15) is rotatably connected to the support plate (14), a support rod (25) is fixedly connected to the heating column (15), and a linkage portion for driving the heating column (15) to rotate is provided on the support plate (14).

4. The stem cell culture device according to claim 3, characterized in that: The linkage part comprises a passive gear (16) fixedly mounted on the bottom of the heating column (15), and a second ring gear (17) meshingly connected with the passive gear (16) is fixedly connected to the partition plate (4).

5. The stem cell culture device according to claim 1, characterized in that: The lower end of the culture dish (6) is provided with a plurality of insertion holes (18), the inner wall of the upper end of the culture dish (6) is provided with a plurality of groups of exhaust holes (19) distributed circumferentially, the exhaust holes (19) are connected to the insertion holes (18), the support plate (8) is fixedly connected with a column (20) inserted into the insertion hole (18), and the support plate (8) is provided with an air supply portion for supplying air to the insertion hole (18).

6. The stem cell culture device according to claim 5, characterized in that: The air supply portion includes a plurality of elastic air bags (23) mounted on the upper edge of the support plate (8), and the plurality of elastic air bags (23) are fixedly connected with an air intake pipe (24) and an air exhaust pipe (32). A one-way valve is fixedly installed in each of the intake pipe (24) and the exhaust pipe (32), the output end of the exhaust pipe (32) extends to the top of the column (20), and an annular plate (22) is fixedly connected to the upper cavity (2) via a bracket (21), and the annular plate (22) is located above the multiple elastic airbags (23).

7. The stem cell culture device according to claim 6, characterized in that: A heating wire (26) is fixedly mounted on the inner bottom of the lower cavity (3), and the heating wire (26) has a circular ring shape.

8. The stem cell culture device according to claim 7, characterized in that: The end of the air suction pipe (24) extends to the inner top of the lower cavity (3); the top of the incubator (1) is provided with an air inlet (28) extending into the lower cavity (3); the sealing cover (30) is provided with an air outlet; and one-way valves are installed in both the air inlet (28) and the air outlet.

9. The stem cell culture device according to claim 8, characterized in that: The bottom of the incubator (1) is fixedly connected to a vertical pipe (27), the air inlet end of the air inlet hole (28) is located at the upper end of the vertical pipe (27), and a filter element (29) is installed in the lower end of the vertical pipe (27).

10. A stem cell culture method, comprising a stem cell culture device according to any one of claims 1 to 9, characterized in that: The steps are as follows: Step 1: Place the stem cells to be cultured and nutrients in a culture dish (6), and allow the incubator (1) to reach a preset culture environment; Step 2: Make the support plate (8) simulate manual shaking of the culture dish (6) on its upper end; Step 3: causing the plurality of heating columns (15) and the support rods (25) to revolve around the culture dish (6); Step 4: The heating column (15) drives the support rod (25) to revolve around the axis of the heating column (15); Step 5: Allow the nutrients in the culture dish (6) to be blown by air.

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