Cell culture dish separation device and process

Abstract

The invention discloses a cell culture dish separation device and process, and relates to the technical field of cell culture, the cell culture dish separation device comprises a box body, the box body is rotatably connected with a box door, the middle position of the inner wall of the box body is fixedly connected with a partition plate, the partition plate is internally provided with a culture dish body, and the two sides of the culture dish body are fixedly connected with grips; the device further comprises a butt joint assembly, an adjusting assembly and a dismounting assembly, the butt joint assembly is arranged in the box body and used for installing and using the culture dish body, and the adjusting assembly is arranged in the box body and used in cooperation with the butt joint assembly. The dismounting assembly is arranged in the culture dish body and is used in cooperation with the culture dish body. The culture dish is reasonable in structure, the culture dish body can be conveniently and rapidly mounted through cooperative use of the butt joint assembly and the adjusting assembly, and then a pore plate in the culture dish body can be taken down through the dismounting assembly.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a cell culture dish separation device and process. Background Art

[0002] Cell culture dishes have good transparency, are non-toxic, and sterile, and can promote the growth and reproduction of primary cells such as nerve cells, passaged cells such as tumor cells, and other animal and human cells. Currently, cell culture plates are often designed as one-piece porous structures, with several fixed cell culture dishes set on the cell culture plate. During single cell culture, the culture personnel place the cells in the cell culture dish so that the same cells can grow and reproduce in a balanced environment.

[0003] When existing cell culture dishes are placed inside a closed box for culture work, most of them are placed inside the box by pulling out. This setting method makes it convenient for the staff to quickly take out the cell culture dishes later. Summary of the Invention

[0004] The purpose of this application is to provide a cell culture dish separation device and process, which facilitates the rapid installation of the culture dish body through the coordinated use of the docking component and the adjustment component, and then the well plate inside the culture dish body can be removed by disassembling the component.

[0005] To achieve the above-mentioned objectives, the present application provides the following technical solutions: a cell culture dish separation device and process, comprising a box body, a box door being rotatably connected to the box body, a partition being fixedly connected to the middle position of the inner wall of the box body, a culture dish body being arranged inside the partition, and handles being fixedly connected on both sides of the culture dish body; further comprising a docking assembly, an adjustment assembly and a disassembly assembly, the docking assembly being arranged inside the box body for installing and using the culture dish body, the adjustment assembly being arranged inside the box body for use in conjunction with the docking assembly, and the disassembly assembly being arranged inside the culture dish body for use in conjunction.

[0006] Preferably, the docking assembly includes a support plate fixedly mounted on the inner wall of the box, the other end of the support plate is fixedly connected to the partition, a through hole is provided on the support plate, and a cross bar is fixedly connected to the inner wall of the through hole.

[0007] Preferably, the bottom surface of the support plate is fixedly connected to a base, a sliding rod is fixedly connected between the bases, a sleeve block is slidably connected to the sliding rod, the bottom surface of the sleeve block is rotatably connected to a pulley, the middle end of the sliding rod is fixedly connected to a ring, a spring is sleeved on the sliding rod, and both ends of the spring are fixedly connected to one side of the sleeve block and one side of the ring respectively.

[0008] Preferably, a docking plate is fixedly connected to the top of the sleeve block, the docking plate is slidably connected to the cross bar, a clamping block is fixedly connected to the top of the docking plate, and a clamping slot is provided on one side of the clamping block.

[0009] Preferably, the adjustment assembly includes a column rotatably connected to the bottom wall of the box, an arm plate is fixedly connected to the column, a slide groove is provided at one end of the arm plate, and the pulley is slidably connected inside the slide groove.

[0010] Preferably, a gear is fixedly connected to the column, a tooth plate is meshed on the gear, one end of the tooth plate is fixedly connected to the driven plate, a push rod is fixedly connected to the middle position of one side of the driven plate, one end of the push rod is movably inserted into the box, and one end of the push rod is fixedly connected to a handle.

[0011] Preferably, the disassembly assembly includes a bracket fixedly installed at the bottom position of the culture dish body, a pair of clamping rods are fixedly connected to the bracket, the middle position of the pair of clamping rods is clamped inside the clamping slot, a liquid collecting plate is provided inside the culture dish body, and a liquid collecting trough is provided on the liquid collecting plate.

[0012] Preferably, a perforated plate is provided inside the culture dish body, and a plurality of groups of circular holes are provided on the perforated plate. A diversion block is fixedly installed on the surface of the liquid collecting tank. Side plates are fixedly connected to both sides of the perforated plate, and pins are fixedly connected to the side plates. L-shaped frames are fixedly connected to both sides of the culture dish body, and a docking groove is provided on the L-shaped frame. The pin is clamped inside the docking groove.

[0013] Preferably, shaft seats are fixedly connected on both sides of the orifice plate, a rod body is fixedly connected to the shaft seat, one end of the rod body is fixedly connected to a limiting plate, several groups of inclined grooves are provided on the limiting plate, one of the inclined grooves is provided with a clamping column inside, and one end of the clamping column is fixedly connected to the culture dish body.

[0014] The present invention also provides a cell culture dish separation process, comprising: The cell culture fluid is added to the orifice plate inside the culture dish body. There are several groups of circular holes on the orifice plate, and the diameters of the circular holes are different. Since the orifice plate is set at an angle, the cell solution will flow on the orifice plate and fall through the circular holes into the liquid collection tank for collection, thereby facilitating the separation of the cell culture fluid.

[0015] In summary, the present invention has the following beneficial effects: 1. The present invention has a reasonable structure. When the handle needs to be taken out, the handle on the adjustment assembly is pushed to make the sleeve slide on the slide rod. A spring is sleeved on the slide rod. The spring is pulled by the movement of the sleeve. A docking plate is fixedly connected to the top of the sleeve, and a clamping block is fixedly connected to the top of the docking plate. The movement of the sleeve drives the clamping block to move through the docking plate. A clamping slot is provided on one side of the clamping block. A clamping rod is clamped inside the clamping slot. The clamping rod is separated from the inside of the clamping slot by the movement of the clamping block, thereby facilitating the removal of the handle. 2. In the present invention, when the sleeve block is moved, the handle is pushed to move the push rod, and the movement of the push rod pushes the driven plate, which is fixedly connected to the driven plate, and the toothed plate and the gear are meshed with each other. The movement of the driven plate drives the gear to rotate through the toothed plate, and the rotation of the gear drives the column to rotate. The column is fixedly connected to an arm plate, and the arm plate is provided with a slide groove. The rotation of the arm plate causes the pulley to slide inside the slide groove, thereby facilitating the movement of the sleeve block; 3. In the present invention, when the orifice plate needs to be installed, side plates are fixedly connected on both sides of the orifice plate, and pins are fixedly connected to the side plates. The pins are clamped into the inside of the docking groove to preliminarily fix the position of the orifice plate, and then the orifice plate is placed as a whole inside the culture dish body. Axle seats are fixedly connected on both sides of the orifice plate, and a rod body is fixedly connected to the axle seat. One end of the rod body is rotatably connected to the limit plate, and the inclined groove on the limit plate is clamped on the clamping column, so as to facilitate limiting the position of the orifice plate. The clamping column is opened in different inclined grooves, and the inclination of the orifice plate will also be adjusted. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 Schematic diagram of the three-dimensional structure of the box; Figure 2 It is a schematic diagram of the three-dimensional structure of the box from the rear; Figure 3 It is a schematic diagram of the three-dimensional structure of a partial section of the box; Figure 4 This is a schematic diagram of the three-dimensional structure of the support plate when viewed from above; Figure 5 This is a schematic diagram of the three-dimensional structure of the culture dish body when viewed from above; Figure 6 Schematic diagram of the three-dimensional structure of the culture dish; Figure 7Schematic diagram of the internal structure of the culture dish; Figure 8 for Figure 6 Enlarged structural diagram at point A in the middle.

[0018] In the figure: 1. Box body; 101. Box door; 102. Partition; 103. Culture dish body; 104. Handle; 2. Support plate; 201. Through hole; 202. Crossbar; 203. Base; 204. Slide bar; 205. Bushing; 206. Pulley; 207. Ring; 208. Spring; 209. Docking plate; 210. Block; 211. Slot; 3. Column; 301. Arm plate; 302. Slot; 303. Gear Wheel; 304, tooth plate; 305, driven plate; 306, push rod; 307, handle; 4, bracket; 401, clamping rod; 402, liquid collecting plate; 403, liquid collecting tank; 404, orifice plate; 405, circular hole; 406, diverter block; 407, side plate; 408, pin; 409, L-shaped frame; 410, docking groove; 411, shaft seat; 412, rod body; 413, limit plate; 414, inclined groove; 415, clamping column. DETAILED DESCRIPTION

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] Example: Reference Figure 1 - Figure 8 The device and process for separating a cell culture dish shown include a box body 1, to which a door 101 is rotatably connected, a partition 102 is fixedly connected to the middle position of the inner wall of the box body 1, a culture dish body 103 is arranged inside the partition 102, and handles 104 are fixedly connected on both sides of the culture dish body 103; it also includes a docking assembly, an adjustment assembly and a disassembly assembly. The docking assembly is arranged inside the box body 1 for installing and using the culture dish body 103, the adjustment assembly is arranged inside the box body 1 for use in conjunction with the docking assembly, and the disassembly assembly is arranged inside the culture dish body 103 for use in conjunction.

[0021] Specifically, it should be noted that the cell solution is added to the orifice plate 404. Since the orifice plate 404 is set at an angle, a circular hole 405 is opened on the orifice plate 404. The solution on the orifice plate 404 enters the collection tank 403 through the circular hole 405 for separation, and the cell solution is separated and collected through the circular holes 405 with different pore sizes.

[0022] As an implementation method in this embodiment, the docking assembly includes a support plate 2 fixedly mounted on the inner wall of the box body 1, the other end of the support plate 2 is fixedly connected to the partition 102, a through hole 201 is opened on the support plate 2, a cross bar 202 is fixedly connected to the inner wall of the through hole 201, the bottom surface of the support plate 2 is fixedly connected to the base 203, a slide bar 204 is fixedly connected between the bases 203, a sleeve block 205 is slidably connected to the slide bar 204, and the bottom surface of the sleeve block 205 is rotatably connected It is connected to a pulley 206, and a ring 207 is fixedly connected to the middle end of the slide rod 204. A spring 208 is sleeved on the slide rod 204. The two ends of the spring 208 are respectively fixedly connected to one side of the sleeve block 205 and one side of the ring 207. The top position of the sleeve block 205 is fixedly connected to a docking plate 209, and the docking plate 209 is slidably connected to the cross bar 202. The top position of the docking plate 209 is fixedly connected to a clamping block 210, and a clamping slot 211 is provided on one side of the clamping block 210.

[0023] Specifically, when the handle 104 needs to be taken out, the handle 307 on the adjustment component is pushed to make the sleeve block 205 slide on the slide bar 204. A spring 208 is sleeved on the slide bar 204, and the spring 208 is pulled by the movement of the sleeve block 205. A docking plate 209 is fixedly connected to the top position of the sleeve block 205, and a clamping block 210 is fixedly connected to the top of the docking plate 209. The movement of the sleeve block 205 drives the clamping block 210 to move through the docking plate 209. A clamping slot 211 is provided on one side of the clamping block 210, and a clamping rod 401 is clamped inside the clamping slot 211. The clamping rod 401 is disengaged from the inside of the clamping slot 211 by the movement of the clamping block 210, thereby facilitating the taking out of the handle 104.

[0024] As an implementation method in this embodiment, the adjustment component includes a column 3 rotatably connected to the bottom wall of the box body 1, an arm plate 301 is fixedly connected to the column 3, a slide groove 302 is opened at one end of the arm plate 301, and a pulley 206 is slidably connected inside the slide groove 302, a gear 303 is fixedly connected to the column 3, a tooth plate 304 is engaged with the gear 303, one end of the tooth plate 304 is fixedly connected to the driven plate 305, a push rod 306 is fixedly connected to the middle position of one side of the driven plate 305, one end of the push rod 306 is movably inserted into the box body 1, and one end of the push rod 306 is fixedly connected to the handle 307.

[0025] Specifically, when the sleeve block 205 is moved, the handle 307 is pushed to move the push rod 306, and the movement of the push rod 306 pushes the driven plate 305. The driven plate 305 is fixedly connected to the tooth plate 304, and the tooth plate 304 and the gear 303 are engaged with each other. The movement of the driven plate 305 drives the gear 303 to rotate through the tooth plate 304, and the rotation of the gear 303 drives the column 3 to rotate. The column 3 is fixedly connected to the arm plate 301, and the arm plate 301 is provided with a slide groove 302. The rotation of the arm plate 301 causes the pulley 206 to slide inside the slide groove 302, thereby facilitating the movement of the sleeve block 205.

[0026] As an implementation method in this embodiment, the disassembly component includes a bracket 4 fixedly installed at the bottom position of the culture dish body 103, a pair of clamping rods 401 are fixedly connected to the bracket 4, the middle position of the pair of clamping rods 401 is clamped inside the clamping slot 211, a liquid collecting plate 402 is provided inside the culture dish body 103, a liquid collecting trough 403 is provided on the liquid collecting plate 402, a hole plate 404 is provided inside the culture dish body 103, a plurality of groups of circular holes 405 are provided on the hole plate 404, a diversion block 406 is fixedly installed on the surface of the liquid collecting trough 403, and side plates 4 are fixedly connected on both sides of the hole plate 404. 07, a pin 408 is fixedly connected to the side plate 407, and an L-shaped frame 409 is fixedly connected to both sides of the culture dish body 103. A docking groove 410 is provided on the L-shaped frame 409, and the pin 408 is clamped inside the docking groove 410. A shaft seat 411 is fixedly connected to both sides of the orifice plate 404, and a rod body 412 is fixedly connected to the shaft seat 411. One end of the rod body 412 is fixedly connected to a limit plate 413. A plurality of groups of inclined grooves 414 are provided on the limit plate 413, and a clamping column 415 is clamped inside one of the inclined grooves 414, and one end of the clamping column 415 is fixedly connected to the culture dish body 103.

[0027] Specifically, when the orifice plate 404 needs to be installed, side plates 407 are fixedly connected on both sides of the orifice plate 404, and pins 408 are fixedly connected to the side plates 407. The pins 408 are clamped into the inside of the docking grooves 410 to preliminarily fix the position of the orifice plate 404. Then the orifice plate 404 is placed as a whole inside the culture dish body 103. Shaft seats 411 are fixedly connected on both sides of the orifice plate 404, and a rod body 412 is fixedly connected to the shaft seat 411. One end of the rod body 412 is rotatably connected to the limiting plate 413, and the inclined groove 414 on the limiting plate 413 is clamped on the clamping column 415, so as to facilitate limiting the position of the orifice plate 404. The clamping column 415 is opened in different inclined grooves 414, and the inclination of the orifice plate 404 can also be adjusted.

[0028] Working principle of the present invention: When the grip 104 needs to be taken out, the handle 307 on the adjustment assembly is pushed to make the sleeve block 205 slide on the slide rod 204. The slide rod 204 is sleeved with a spring 208. The spring 208 is pulled by the movement of the sleeve block 205. A docking plate 209 is fixedly connected to the top position of the sleeve block 205, and a clamping block 210 is fixedly connected to the top of the docking plate 209. The movement of the sleeve block 205 drives the clamping block 210 to move through the docking plate 209. A clamping slot 211 is provided on one side of the clamping block 210. A clamping rod 401 is clamped inside the clamping slot 211. The clamping rod 401 is disengaged from the inside of the clamping slot 211 by the movement of the clamping block 210, thereby facilitating the taking out of the grip 104. When the sleeve block 205 is moved, the handle 307 is pushed to move the push rod 306, and the movement of the push rod 306 pushes the driven plate 305. The driven plate 305 is fixedly connected to the toothed plate 304, and the toothed plate 304 and the gear 303 are meshed with each other. The movement of the driven plate 305 drives the gear 303 to rotate through the toothed plate 304, and the rotation of the gear 303 drives the column 3 to rotate. The column 3 is fixedly connected to the arm plate 301, and the arm plate 301 is provided with a slide groove 302. The rotation of the arm plate 301 causes the pulley 206 to slide inside the slide groove 302, thereby facilitating the movement of the sleeve block 205. When the orifice plate 404 needs to be installed, side plates 407 are fixedly connected on both sides of the orifice plate 404, and pins 408 are fixedly connected to the side plates 407. The pins 408 are clamped into the inside of the docking grooves 410 to preliminarily fix the position of the orifice plate 404. Then the orifice plate 404 is placed as a whole inside the culture dish body 103. Axle seats 411 are fixedly connected on both sides of the orifice plate 404, and a rod body 412 is fixedly connected to the shaft seat 411. One end of the rod body 412 is rotatably connected to the limit plate 413, and the inclined groove 414 on the limit plate 413 is clamped on the clamping column 415, so as to facilitate limiting the position of the orifice plate 404. The clamping column 415 is opened in different inclined grooves 414, and the inclination of the orifice plate 404 can also be adjusted.

[0029] The present invention also provides a cell culture dish separation process, comprising: The cell culture fluid is added to the orifice plate 404 inside the culture dish body 103. A plurality of groups of circular holes 405 are provided on the orifice plate 404, and the diameters of the circular holes 405 are different. Since the orifice plate 404 is tilted, the cell solution will flow on the orifice plate 404 and fall through the circular holes 405 into the liquid collecting tank 403 for collection, thereby facilitating the separation of the cell culture fluid.

[0030] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cell culture dish separation device, characterized in that: include A box body (1), wherein a box door (101) is rotatably connected to the box body (1), a partition (102) is fixedly connected to the middle position of the inner wall of the box body (1), a culture dish body (103) is provided inside the partition (102), and handles (104) are fixedly connected to both sides of the culture dish body (103); It also includes a docking assembly, an adjustment assembly, and a disassembly assembly. The docking assembly is arranged inside the box (1) for installing and using the culture dish body (103). The adjustment assembly is arranged inside the box (1) for use in conjunction with the docking assembly. The disassembly assembly is arranged inside the culture dish body (103) for use in conjunction with the docking assembly.

2. A cell culture dish separation device according to claim 1, characterized in that: The docking assembly comprises a support plate (2) fixedly mounted on the inner wall of the box body (1), the other end of the support plate (2) being fixedly connected to the partition plate (102), a through hole (201) being provided on the support plate (2), and a cross bar (202) being fixedly connected to the inner wall of the through hole (201).

3. The cell culture dish separation device according to claim 2, characterized in that: The bottom surface of the support plate (2) is fixedly connected to a base (203), a slide bar (204) is fixedly connected between the bases (203), a sleeve block (205) is slidably connected to the slide bar (204), the bottom surface of the sleeve block (205) is rotatably connected to a pulley (206), a sleeve ring (207) is fixedly connected to the middle end of the slide bar (204), a spring (208) is sleeved on the slide bar (204), and two ends of the spring (208) are fixedly connected to one side of the sleeve block (205) and one side of the sleeve ring (207), respectively.

4. The cell culture dish separation device according to claim 3, characterized in that: A docking plate (209) is fixedly connected to the top of the sleeve block (205), and the docking plate (209) is slidably connected to the crossbar (202). A clamping block (210) is fixedly connected to the top of the docking plate (209), and a clamping slot (211) is provided on one side of the clamping block (210).

5. The cell culture dish separation device according to claim 3, characterized in that: The adjustment assembly comprises a column (3) rotatably connected to the bottom wall of the box (1), an arm plate (301) being fixedly connected to the column (3), a sliding groove (302) being provided at one end of the arm plate (301), and the pulley (206) being slidably connected inside the sliding groove (302).

6. The cell culture dish separation device according to claim 5, characterized in that: A gear (303) is fixedly connected to the column (3), a toothed plate (304) is meshed with the gear (303), one end of the toothed plate (304) is fixedly connected to a driven plate (305), a push rod (306) is fixedly connected to the middle position of one side of the driven plate (305), one end of the push rod (306) is movably inserted into the box (1), and one end of the push rod (306) is fixedly connected to a handle (307).

7. The cell culture dish separation device according to claim 4, characterized in that: The disassembly assembly comprises a bracket (4) fixedly mounted at the bottom of the culture dish body (103); a pair of clamping rods (401) are fixedly connected to the bracket (4); the middle positions of the pair of clamping rods (401) are clamped inside the clamping slot (211); a liquid collecting plate (402) is provided inside the culture dish body (103); and a liquid collecting trough (403) is provided on the liquid collecting plate (402).

8. The cell culture dish separation device according to claim 7, characterized in that: A hole plate (404) is provided inside the culture dish body (103), and a plurality of groups of circular holes (405) are provided on the hole plate (404). A diversion block (406) is fixedly installed on the surface of the liquid collecting tank (403). Side plates (407) are fixedly connected on both sides of the hole plate (404), and pins (408) are fixedly connected on the side plates (407). L-shaped frames (409) are fixedly connected on both sides of the culture dish body (103), and a docking groove (410) is provided on the L-shaped frame (409). The pins (408) are clamped inside the docking groove (410).

9. The cell culture dish separation device according to claim 8, characterized in that: Both sides of the orifice plate (404) are fixedly connected to shaft seats (411), and a rod body (412) is fixedly connected to the shaft seat (411). One end of the rod body (412) is fixedly connected to a limiting plate (413), and the limiting plate (413) is provided with a plurality of groups of inclined grooves (414), wherein a clamping column (415) is provided inside one of the inclined grooves (414), and one end of the clamping column (415) is fixedly connected to the culture dish body (103).

10. A cell culture dish separation process, based on any one of claims 1 to 9, comprising: The cell culture fluid is added to the orifice plate (404) inside the culture dish body (103). A plurality of groups of circular holes (405) are provided on the orifice plate (404), and the diameters of the circular holes (405) are different. Since the orifice plate (404) is tilted, the cell solution will flow on the orifice plate (404) and fall through the circular holes (405) into the interior of the liquid collecting tank (403) for collection, thereby facilitating the separation of the cell culture fluid.

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