Combined metabolic marker for judging degranulation inhibition effect of tea-derived polyphenol substance based on rat basophilic leukemia cells and detection kit thereof

By combining fatty acids (FA22:5), phosphatidylcholine (PC 38:2) and phosphatidylcholine (PC O-30:0) as metabolic markers, combined with chromatography-mass spectrometry technology and bioinformatics analysis, the detection problem of tea polyphenols on degranulation of basophilic leukemia cells in rats was solved, and high sensitivity and efficient discrimination effect was achieved.

CN120446505APending Publication Date: 2025-08-08JIMEI UNIV
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Patent Information

Application Number
CN202510490965.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-18
Publication Date
2025-08-08

AI Technical Summary

Technical Problem

The prior art has not yet effectively used the combined detection of lipid metabolites to determine the inhibitory effect of tea-derived polyphenols on rat basophilic leukemia cells, resulting in insufficient detection sensitivity and specificity.

Method used

The combined use of combined metabolic markers fatty acids (FA22:5), phosphatidylcholine (PC 38:2) and phosphatidylcholine (PC O-30:0), combined with ultra-high performance liquid chromatography-mass spectrometry combined technology and bioinformatics analysis, was used to determine the degranulation inhibitory effect of polyphenols from tea from the Binary Logistic Regression formula P value.

Benefits of technology

It has achieved high sensitivity and efficient detection, and has low cost and good repeatability to determine the inhibitory effect of degranulation of polyphenols from tea-derived sources, and has good application prospects.

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Abstract

The invention relates to a combined metabolic marker for judging the degranulation inhibition effect of tea-derived polyphenol substances based on rat basophilic leukemia cells and a detection kit thereof. The method comprises the following steps: detecting relative concentrations of lipid metabolites fatty acid (FA22: 5), phosphatidylcholine (PC 38: 2) and phosphatidylcholine (PCO-30: 0) in a rat basophilic leukemia cell sample, calculating a combined marker variable P and a determined intercept point value based on a binary logistic regression equation, and judging the degranulation inhibition effect of the tea-derived polyphenol substance. The kit can realize high-sensitivity and high-efficiency detection of the three small molecule metabolites involved in the invention, and has the characteristics of low detection cost and good repeatability. The combined use of the three small molecule metabolites has a good application prospect.
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