Method for cultivating cherry seedlings in short period

Abstract

The invention discloses a method for cultivating cherry seedlings in a short period, and relates to the technical field of cherry seedling cultivation. Semi-lignified cherry branches are selected, disinfected and then soaked in composite induction liquid for 2-4 h; peat soil, perlite and humus soil are mixed according to the volume ratio of 3: 1: 1, then naphthylacetic acid powder is added till the content of the naphthylacetic acid powder is 0.1-0.3 g / L, and a cutting medium is obtained; the explants are inserted into a cutting medium to be cultured; an LED light source with the ratio of red light to blue light being 7: 3 is adopted, illumination is conducted for 12 h every day, and the light intensity is 800-1200 lux. Through the synergistic effect of the scheme, the cultivation period is shortened, and the survival rate is remarkably increased.

Description

Technical Field

[0001] The invention relates to the technical field of cherry seedling cultivation, and more particularly to a method for cultivating cherry seedlings in a short period. Background Art

[0002] In the cherry cultivation industry, cultivating high-quality cherry seedlings is a key step in ensuring cherry yield and quality. With the growing market demand for cherries, traditional cultivation methods are no longer able to meet the rapid growth of the industry, requiring both quantity and quality.

[0003] Traditional cherry seedling cultivation methods mostly rely on seedling propagation, cuttings, or grafting. Seedling propagation has a long cycle, often requiring three to five years from sowing to producing transplantable seedlings. Furthermore, the offspring exhibit significant trait segregation, making it difficult to maintain high-quality characteristics. Cutting propagation is significantly affected by seasonal and environmental factors, resulting in an unstable survival rate of only 50% to 70%. Rooting is also slow, and the cultivation cycle typically requires two to three years. Grafting, however, requires stringent selection of rootstock and scion, is complex, with a grafting survival rate of only 60% to 80%, and a similarly long cultivation cycle.

[0004] Therefore, there is an urgent need for a cherry seedling cultivation method that can shorten the cultivation cycle, improve the survival rate and seedling quality. Summary of the Invention

[0005] In view of this, the present invention provides a method for cultivating cherry seedlings in a short cycle.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] An embodiment of the present invention provides a method for cultivating cherry seedlings in a short cycle, comprising the following steps:

[0008] (1) Explant pretreatment: Select semi-lignified cherry branches, disinfect them, and soak them in a composite induction solution for 2-4 hours; wherein the composite induction solution contains the following ingredients: 0.1-0.5 mg / L GA3, 0.05-0.2 mg / L NAA, 0.01-0.05 mg / L BR, and 5-10 g / L sucrose;

[0009] (2) Preparation of cutting medium: peat soil, perlite and humus soil were mixed in a volume ratio of 3:1:1, and then naphthaleneacetic acid powder was added to a content of 0.1-0.3 g / L to obtain a cutting medium;

[0010] (3) Cutting and staged culture: inserting the explants pretreated in step (1) into the cutting medium obtained in step (2) for culture;

[0011] (4) Photoperiod control: Use LED light source with red light: blue light = 7:3, 12 hours of light per day, and light intensity of 800-1200 lux.

[0012] Preferably, the composite induction solution further contains 0.002-0.01 mg / L TDZ.

[0013] Preferably, in step (2), biochar with a volume fraction of 0.5%-1% is added to the cutting medium.

[0014] Preferably, in step (3), the culture is as follows: after inserting into the cutting medium obtained in step (2), controlling the temperature at 18-22°C and the humidity at 80%-85% for 1-15 days, and spraying the basic nutrient solution; spraying a 2g / L aqueous solution of alginic acid extract once on the 10th day after cutting to relieve oxidative stress; raising the temperature to 23-26°C and the humidity to 70%-75% for 16-30 days, spraying a fortified nutrient solution containing 0.1mg / L paclobutrazol, and supplementing CO2 to 800-1000ppm daily for 2 hours.

[0015] Preferably, the basic nutrient solution contains: 0.01% KH2PO4, 0.005% CaCl2, 0.001% Fe-EDTA, 0.0005% ZnSO4, and the spraying amount is 10-15 mL / plant / time, and it is sprayed once a day.

[0016] Preferably, the fortified nutrient solution is further added with 0.05-0.1 mg / L IBA on the basis of the basic nutrient solution; the spraying amount is 5-8 mL / plant / time, and the spraying is once a day.

[0017] Preferably, the spraying amount of the alginic acid extract aqueous solution is 3-5 mL / plant / time, and it is carried out between 15:00 and 17:00 on the afternoon of the 10th day after cutting.

[0018] Preferably, in step (4), far-red light with a wavelength of 730 nm is applied for 5 minutes every 2 hours to inhibit excessive branch growth.

[0019] Preferably, the seedlings are hardened 7 days before transplanting: the humidity is reduced by 5% and the light intensity is reduced by 20% every day.

[0020] Through the above technical solution, it can be seen that the technical effects achieved by the present invention are:

[0021] 1) The present invention uses GA3 to break dormancy, NAA to promote cell division, and sucrose to provide energy; the three ingredients synergistically shorten the rooting initiation time by 40%-50%, increase the rooting rate to 85%-92% (50%-70% of the control), and shorten the rooting time to 10-15 days (20-30 days of the control).

[0022] 2) The present invention optimizes the cutting medium and nutrient solution system. The peat soil-perlite-humus soil combination ensures air permeability and water retention. Naphthaleneacetic acid powder continuously stimulates callus formation, thereby increasing the fresh weight of seedlings by 35%-45% and the number of lateral roots by 2-3 times.

[0023] The synergistic effect of the above schemes shortens the cultivation period and significantly improves the survival rate. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Example 1

[0026] A method for cultivating cherry seedlings in a short cycle comprises the following steps:

[0027] (1) Explant pretreatment: semi-lignified cherry branches were selected, disinfected, and then immersed in a composite induction solution for 2 h; wherein the composite induction solution contains the following ingredients: 0.1 mg / L GA3, 0.05 mg / L NAA, 0.01 mg / L BR, 5 g / L sucrose, and 0.002 mg / L TDZ;

[0028] (2) Preparation of cutting medium: peat soil, perlite and humus soil were mixed in a volume ratio of 3:1:1, and then naphthaleneacetic acid powder was added to a content of 0.1 g / L, and biochar was added to a total volume fraction of 0.5% to obtain a cutting medium;

[0029] (3) Cutting and staged culture: The explants pretreated in step (1) are inserted into the cutting medium obtained in step (2) for culture, and the temperature is controlled at 18-22°C and the humidity is 80%-85% for 1-15 days, and the basic nutrient solution is sprayed; on the 10th day after cutting, a 2g / L aqueous solution of alginate extract is sprayed once to relieve oxidative stress; on the 16th-30th day, the temperature is raised to 23-26°C and the humidity is 70%-75%, and a fortified nutrient solution containing 0.1mg / L paclobutrazol is sprayed, and CO2 is supplemented to 800-1000ppm daily for 2 hours; the basic nutrient solution contains: 0.01% KH2PO4, 0.005% CaCl2, 0.001% Fe-EDTA, 0.0005% ZnSO4, and the spraying amount is 10mL / plant / time, and spray once a day. The enhanced nutrient solution was further supplemented with 0.05 mg / L IBA on the basis of the basic nutrient solution; the spraying amount was 5 mL / plant / time, sprayed once a day; the spraying amount of the alginic acid extract aqueous solution was 3 mL / plant / time, and it was carried out from 15:00 to 17:00 on the 10th day after cutting.

[0030] (4) Photoperiod regulation: Use an LED light source with a red light: blue light ratio of 7:3, with a light intensity of 800 lux and 12 hours of illumination per day; during this period, apply far-red light with a wavelength of 730 nm for 5 minutes every 2 hours to inhibit excessive branch growth.

[0031] (5) Harden the seedlings 7 days before transplanting: reduce humidity by 5% and light intensity by 20% daily.

[0032] Example 2

[0033] A method for cultivating cherry seedlings in a short cycle comprises the following steps:

[0034] (1) Explant pretreatment: semi-lignified cherry branches were selected, disinfected, and then immersed in a composite induction solution for 4 h; wherein the composite induction solution contains the following ingredients: 0.5 mg / L GA3, 0.2 mg / L NAA, 0.05 mg / L BR, 10 g / L sucrose, and 0.01 mg / L TDZ;

[0035] (2) Preparation of cutting medium: peat soil, perlite and humus soil were mixed in a volume ratio of 3:1:1, and then naphthaleneacetic acid powder was added to a content of 0.3 g / L, and biochar was added to a total volume fraction of 1% to obtain a cutting medium;

[0036] (3) Cutting and staged culture: The explants pretreated in step (1) are inserted into the cutting medium obtained in step (2) for culture, and the temperature is controlled at 18-22°C and the humidity is 80%-85% for 1-15 days, and a basic nutrient solution is sprayed; on the 10th day after cutting, a 2g / L aqueous solution of alginate extract is sprayed once to relieve oxidative stress; on the 16th-30th day, the temperature is raised to 23-26°C and the humidity is 70%-75%, and a fortified nutrient solution containing 0.1mg / L paclobutrazol is sprayed, and CO2 is supplemented to 800-1000ppm daily for 2 hours; the basic nutrient solution contains: 0.01% KH2PO4, 0.005% CaCl2, 0.001% Fe-EDTA, and 0.0005% ZnSO4, and the spraying amount is 15mL / plant / time, and sprayed once a day. The fortified nutrient solution was supplemented with 0.1 mg / L IBA on top of the basic nutrient solution; the spray rate was 8 mL / plant / time, once daily. The alginic acid extract aqueous solution was sprayed at a rate of 5 mL / plant / time, between 3:00 PM and 5:00 PM on the 10th day after cutting.

[0037] (4) Photoperiod regulation: Use an LED light source with a red light: blue light ratio of 7:3, with a light intensity of 1200 lux for 12 hours per day; during this period, apply 5 minutes of far-red light with a wavelength of 730nm every 2 hours to inhibit excessive branch growth.

[0038] (5) Harden the seedlings 7 days before transplanting: reduce humidity by 5% and light intensity by 20% daily.

[0039] Example 3

[0040] A method for cultivating cherry seedlings in a short cycle comprises the following steps:

[0041] (1) Explant pretreatment: semi-lignified cherry branches were selected, disinfected, and then immersed in a composite induction solution for 2-4 hours; wherein the composite induction solution contains the following ingredients: 0.25 mg / L GA3, 0.1 mg / L NAA, 0.03 mg / L BR, 7 g / L sucrose, and 0.008 mg / L TDZ;

[0042] (2) Preparation of cutting medium: peat soil, perlite and humus soil were mixed in a volume ratio of 3:1:1, and then naphthaleneacetic acid powder was added to a content of 0.2 g / L, and biochar was added to a total volume fraction of 0.75% to obtain a cutting medium;

[0043] (3) Cutting and staged culture: The explants pretreated in step (1) are inserted into the cutting medium obtained in step (2) for culture, and the temperature is controlled at 18-22°C and the humidity is 80%-85% for 1-15 days, and a basic nutrient solution is sprayed; on the 10th day after cutting, a 2g / L aqueous solution of alginate extract is sprayed once to relieve oxidative stress; on the 16th-30th day, the temperature is raised to 23-26°C and the humidity is 70%-75%, and a fortified nutrient solution containing 0.1mg / L paclobutrazol is sprayed, and CO2 is supplemented to 800-1000ppm daily for 2 hours; the basic nutrient solution contains: 0.01% KH2PO4, 0.005% CaCl2, 0.001% Fe-EDTA, and 0.0005% ZnSO4, and the spraying amount is 10-15mL / plant / time, and sprayed once a day. The fortified nutrient solution was supplemented with 0.07 mg / L IBA on top of the basic nutrient solution; the spray rate was 6 mL / plant / time, once daily. The alginic acid extract aqueous solution was sprayed at a rate of 4 mL / plant / time, between 3:00 PM and 5:00 PM on the 10th day after cutting.

[0044] (4) Photoperiod regulation: Use an LED light source with a red light: blue light ratio of 7:3, with a light intensity of 900 lux and 12 hours of illumination per day; during this period, apply far-red light with a wavelength of 730 nm for 5 minutes every 2 hours to inhibit excessive branch growth.

[0045] (5) Harden the seedlings 7 days before transplanting: reduce humidity by 5% and light intensity by 20% daily.

[0046] In order to verify the effects of each treatment in the embodiment of the present invention, the following comparative experiments are designed. The comparative experiment groups and treatment methods are shown in Table 1:

[0047] Table 1 Treatment methods of each control group

[0048]

[0049] Experiments were conducted according to the methods of Example 3 and Comparative Examples 1 to 6. Each group of experiments included 100 explants, each consisting of newly grown stem segments of the "Meizao" cherry variety, approximately 2-3 cm in length. The experiments were conducted according to the aforementioned methods, and the seedling cycle, survival rate, rooting time, number of lateral roots, plant height, and dry matter accumulation were statistically analyzed. See Table 2.

[0050] Table 2

[0051]

[0052] As can be seen from Table 2, the hormones in the present invention have a synergistic effect, and their combined use can significantly promote rooting and reduce the seedling cycle; moreover, the addition of naphthaleneacetic acid powder and biochar to the cutting medium can significantly promote the number and rooting rate of lateral roots, which indicates that there is also a certain synergistic effect between naphthaleneacetic acid powder and biochar.

[0053] At the same time, comparative examples 7 and 8 were set up; the grouping treatment conditions and the final root activity (μg TTC / g·h) and chlorophyll SPAD values are shown in Table 3.

[0054] Table 3

[0055]

[0056]

[0057] It can be seen from Table 3 that the spraying amount of basic nutrient solution and enhanced nutrient solution has an important impact on the final root activity and leaf photosynthesis.

[0058] The various embodiments in the specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to in detail.

[0059] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for cultivating cherry seedlings in a short cycle, characterized in that: The steps include: (1) Explant pretreatment: semi-lignified cherry branches were selected, disinfected, and then immersed in a composite induction solution for 2-4 hours; wherein the composite induction solution comprises the following components: 0.1-0.5 mg / L GA3, 0.05-0.2 mg / L NAA, 0.01-0.05 mg / L BR, and 5-10 g / L sucrose; (2) Preparation of cutting medium: peat soil, perlite and humus soil were mixed in a volume ratio of 3:1:1, and then naphthaleneacetic acid powder was added to a content of 0.1-0.3 g / L to obtain a cutting medium; (3) Cutting and staged culture: inserting the explants pretreated in step (1) into the cutting medium obtained in step (2) for culture; (4) Photoperiod control: Use LED light source with red light: blue light = 7:3, 12 hours of light per day, and light intensity of 800-1200 lux.

2. The method according to claim 1, characterized in that The composite induction solution further contains 0.002-0.01 mg / L TDZ.

3. The method according to claim 1, characterized in that In step (2), biochar with a volume fraction of 0.5%-1% is added to the cutting medium.

4. The method according to claim 1, wherein In step (3), the culture is as follows: after inserting into the cutting medium obtained in step (2), the temperature is controlled at 18-22° C. and the humidity is 80%-85% for 1-15 days, and a basic nutrient solution is sprayed; on the 10th day after cutting, a 2 g / L aqueous solution of alginic acid extract is sprayed once to relieve oxidative stress; on the 16th-30th day, the temperature is raised to 23-26° C. and the humidity is 70%-75%, and a fortified nutrient solution containing 0.1 mg / L paclobutrazol is sprayed, and CO2 is supplemented to 800-1000 ppm daily for 2 hours.

5. The method according to claim 4, characterized in that The basic nutrient solution contains: 0.01% KH2PO4, 0.005% CaCl2, 0.001% Fe-EDTA, 0.0005% ZnSO4, and the spraying amount is 10-15 mL / plant / time, and it is sprayed once a day.

6. The method according to claim 5, characterized in that The fortified nutrient solution is further added with 0.05-0.1 mg / L IBA on the basis of the basic nutrient solution; the spraying amount is 5-8 mL / plant / time, and the spraying is once a day.

7. The method according to claim 1, characterized in that The spraying amount of the alginic acid extract aqueous solution is 3-5 mL / plant / time, and it is carried out between 15:00 and 17:00 in the afternoon of the 10th day after cutting.

8. The method according to claim 1, characterized in that In step (4), far-red light with a wavelength of 730 nm is applied for 5 minutes every 2 hours to inhibit excessive branch growth.

9. The method according to claim 1, characterized in that Harden the seedlings 7 days before transplanting: reduce humidity by 5% and light intensity by 20% every day.

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