Refuge structure simulation device based on fish behavioristics and simulation method thereof

Abstract

The invention discloses a refuge structure simulation device based on fish behavioristics and a simulation method thereof, the refuge structure simulation device comprises a transparent simulation cylinder, an infrared night vision camera and a water circulation device, the interior of the transparent simulation cylinder is sequentially divided into a water inlet area, a monitoring area and a water outlet area from left to right by a plurality of criss-cross partition plates; an escape area is arranged in the monitoring area; the water circulation device is located outside the transparent simulation cylinder, the water outlet end of the water circulation device is communicated and connected with the water inlet area through a water inlet, and the water inlet end of the water circulation device is communicated and connected with the water outlet area through a water outlet; the infrared night vision camera is arranged at the periphery of the transparent simulation cylinder, and a camera head of the infrared night vision camera is over against the detection area; the optimal structure parameters of the artificial shelter are scientifically designed and verified, the size and the material of the optimal shelter are determined, effective survival shelter is provided for endangered fishes, and population recovery is promoted.

Description

Technical Field

[0001] The present invention belongs to the technical field of ecological protection and fish behavior research, and more specifically relates to a shelter structure simulation device and a simulation method thereof based on fish behavior. Background Art

[0002] With the aggravation of water environment pollution, more and more fish are in endangered or semi-endangered status. Existing research has mostly focused on the impact of water quality on fish survival, but has ignored the factors that endangered fish cannot escape predators after the escape shelters they receive are affected by the environment and disappear, resulting in a sharp decline in the number of fish. For example, the massive disappearance of coral reefs in the Iron Wire Reef and Ox Horn Reef waters of Hainan has led to the death of a large number of fish in the sea area because they cannot escape predation in the coral reef escape shelters. Therefore, when establishing nature reserves for endangered fish, artificial structures that endangered fish like to escape should be placed to further promote the recovery of endangered fish populations. Summary of the Invention

[0003] Purpose of the invention: In order to overcome the deficiencies in the prior art, the present invention provides a shelter structure simulation device and a simulation method based on fish behavior; to solve the problem that the prior art ignores the key impact of fish escape shelter structures on the protection of endangered fish, through structural design and verification experiments of artificial shelters, the optimal structural parameters are selected, and the volume and material of the most suitable shelter for endangered fish are determined.

[0004] Technical solution: To achieve the above-mentioned purpose, the present invention provides a fish behavior-based shelter structure simulation device and simulation method thereof, comprising a transparent simulation tank, an infrared night vision camera and a water circulation device, wherein the interior of the transparent simulation tank 1 is divided into a water inlet area, a monitoring area and a water outlet area from left to right by a number of crisscross partitions; an escape area is provided in the monitoring area; a water inlet is provided on the wall of the water inlet area near the bottom of the transparent simulation tank, and a water outlet is provided on the wall of the water outlet area near the top of the transparent simulation tank; the water circulation device is located outside the transparent simulation tank, and the water circulation device is provided outside the transparent simulation tank. The water outlet end of the device is connected to the water inlet area through the water inlet, and the water inlet end of the water circulation device is connected to the water outlet area through the water outlet; the infrared night vision camera is arranged around the transparent simulation tank, and the camera head of the infrared night vision camera is facing the detection area; the infrared night vision camera can monitor and record the average number of times per minute that the experimental fish in the transparent simulation tank enter the escape area, the swimming speed of the experimental fish in the escape area and the swimming speed of the experimental fish in the detection area, the duration of each entry of the experimental fish into the escape area, the total time the experimental fish stays in the escape area, and the time taken by the experimental fish from the application of stimulation to the first entry into the escape area.

[0005] Furthermore, the escape zone is located on one side of the monitoring area close to the water outlet area, and the escape zone is surrounded by a number of barrier columns and partitions. The volume of the escape zone is 1 / 24 to 1 / 3 of the volume of the monitoring area.

[0006] Furthermore, a plurality of lighting devices are provided at the intersection of the escape zone and the monitoring zone, each of the lighting devices including a light source device, a color-changing device and a dimming knob, the color-changing device is arranged along the lighting direction of the light source device, and the light-transmitting area of the color-changing device covers the light-emitting end of the light source device, and the dimming knob is electrically connected between the light source device and the power supply device; the dimming knob can adjust the magnitude of the current input into the light source device, and the light-emitting end of the light source device irradiates light of different colors into the monitoring area through the light-transmitting area of the color-changing device.

[0007] Furthermore, a pollutant supply device is provided in the area of the water inlet area near the top of the transparent simulation cylinder, and the discharge end of the pollutant supply device is connected to the monitoring area through the partition and discharges pollutants into the monitoring area.

[0008] Furthermore, a turbidity detection and filtering unit is provided on one side of the water outlet located in the water outlet area, and the turbidity detection and filtering unit includes a turbidity meter, a filtering device, a first flow pipe, and a second flow pipe. The turbidity meter is arranged around the water outlet, and the filtering device is arranged on the second flow pipe. The inflow end of the first flow pipe and the inflow end of the second flow pipe are respectively provided with a first solenoid valve and a second solenoid valve, and the outflow end of the first flow pipe and the outflow end of the second flow pipe are both connected to the water inlet end of the water circulation device through the water outlet; the turbidity meter can monitor the turbidity in the water body of the transparent water tank in real time, and control the connection or closure of the first solenoid valve and the second solenoid valve respectively, and at the same time, only one of the first solenoid valve and the second solenoid valve is in a connected state.

[0009] Furthermore, a third solenoid valve is provided at the discharge end of the pollutant supply device, and the turbidity meter can control the connection or closing of the third solenoid valve.

[0010] Furthermore, the partition and the bottom of the transparent simulation tank together enclose a sealed temperature control area, and a temperature controller is provided in the temperature control area, and the temperature controller can heat the water in the transparent simulation tank.

[0011] Furthermore, a residual chlorine detection and adjustment device is provided on one side of the water inlet located in the water inlet area, and the residual chlorine detection and adjustment device can detect and adjust the residual chlorine content in the water body of the transparent simulation tank.

[0012] Furthermore, it also includes a dissolved oxygen detection and adjustment device. The partition and the top of the transparent simulation tank together enclose an oxygen control area. The dissolved oxygen detection and adjustment device is arranged in the oxygen control area, and the detection end and the oxygen supply end of the dissolved oxygen detection and adjustment device both pass through the partition and extend into the monitoring area. The dissolved oxygen detection and adjustment device can detect and adjust the dissolved oxygen content in the water body of the transparent simulation tank.

[0013] Furthermore, a simulation method of a shelter structure simulation device based on fish behavior includes the following steps:

[0014] Step 1: Build an escape zone within the monitoring area in the transparent simulation tank;

[0015] Step 2: Pour water into the transparent simulation tank;

[0016] Step three: regulating the residual chlorine parameters in the water body through the residual chlorine detection and adjustment device; regulating the water temperature through the thermostat; regulating the dissolved oxygen content in the water body through the dissolved oxygen detection and adjustment device; the turbidity meter controls the third solenoid valve on the discharge end of the pollutant supply device to be in a connected state, so that the pollutant supply device is in a connected state with the monitoring area, and non-toxic endocrine disruptors and sediment are put into the monitoring area; turning on the light source device, the turbidity meter detects the turbidity in the water body under the action of light, and controls the first solenoid valve to be in a connected state or the second solenoid valve to be in a connected state according to the detection result; connecting the water inlet end of the water circulation device to the water outlet of the transparent simulation cylinder, connecting the water outlet end of the water circulation device to the water inlet of the transparent simulation cylinder, and controlling the water flow rate in the transparent simulation cylinder through the water circulation device; thereby completing the preset of the standard test environment;

[0017] Step 4: Based on step 3, place several experimental fish in a transparent simulation tank with a male-female ratio of 1:1;

[0018] Step 5: Start the infrared night vision camera so that it can monitor and record the behavior of the experimental fish in the transparent simulation tank in the water body;

[0019] Step 6: Change the color of the light irradiated by the light source device to the monitoring area through the color changing device; under the same light color, adjust the luminous intensity of the light source device through the dimming knob;

[0020] Step 7: Change the volume of the escape zone;

[0021] Step 8: Change the material of the obstacle pillars that make up the escape zone;

[0022] Step 9: Conduct comparative experimental analysis, including:

[0023] a) Each independent experiment is allowed to change only a single variable condition in step 6, step 7 or step 8;

[0024] b) When executing step 6, step 7 or step 8, it is necessary to add toxic heavy metal pollutants into the transparent simulation tank through the pollutant supply device after the same interval of time as the experimental fish is placed in the transparent simulation tank 1;

[0025] c) When executing step 6, step 7 or step 8, it is necessary to re-execute steps 1 to 6 to re-establish the standard test environment.

[0026] Beneficial effects: The shelter structure simulation device and simulation method based on fish behavior of the present invention, by combining the structural parameters (volume, material) of artificial shelters with fish behavior, breaks through the limitation of existing technology that only focuses on water quality, and provides a quantifiable solution to ecological crises such as coral reef degradation; by reducing the environmental pressure of endangered fish through human intervention, the threat of competition from the same species and predators is reduced; and the structural escape structure finally formed according to the experimental results of the present invention can not only be used for the protection of endangered fish, but also can be used for regional fish farming, which can greatly increase the reproduction efficiency of fish. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a structural schematic diagram of the simulation device of the present invention;

[0028] Figure 2 This is a schematic structural diagram of the transparent simulation tank and the water circulation device of the present invention in a connected state;

[0029] Figure 3 This is a diagram showing the device distribution of the transparent simulation cylinder of the present invention;

[0030] Figure 4 It is a schematic structural diagram of the escape zone and the illumination device of the present invention;

[0031] Figure 5 A half-sectional view of a turbidity detection filter unit of the present invention;

[0032] Figure 6 This is a diagram of the experimental results for determining the scope of the escape zone through experiments;

[0033] Figure 7 This is the experimental result diagram of changing the lighting conditions alone;

[0034] Figure 8 This is the experimental result of changing the escape zone volume alone;

[0035] Figure 9 This is the experimental result of changing the material of the obstruction column alone. DETAILED DESCRIPTION

[0036] The present invention will be further described below with reference to the accompanying drawings.

[0037] like Figures 1 to 3 As shown; a shelter structure simulation device based on fish behavior and a simulation method thereof, comprising a transparent simulation cylinder 1, an infrared night vision camera 2 and a water circulation device 3, wherein the interior of the transparent simulation cylinder 1 is divided into a water inlet area 5, a monitoring area 6 and a water outlet area 7 from left to right by a number of crisscross partitions 4; a water inlet 8 is provided on the cylinder wall of the water inlet area 5 near the bottom area of the transparent simulation cylinder 1, and a water outlet 9 is provided on the cylinder wall of the water outlet area 7 near the top area of the transparent simulation cylinder 1; the water circulation device 3 is located outside the transparent simulation cylinder 1, and the water outlet end of the water circulation device 3 is connected to the water inlet area 5 through the water inlet 8, and the water inlet end of the water circulation device 3 is connected to the water outlet area 7 through the water outlet 9; the infrared night vision camera 2 is arranged in the transparent simulation cylinder 1 surrounding area, and the camera of the infrared night vision camera 2 is facing the detection area 6; when the water circulation device 3 is started to make the water in the transparent simulation tank 1 flow, the infrared night vision camera 2 can monitor and record the behavior of the experimental fish in the water of the transparent simulation tank 1; after the video recorded by the infrared night vision camera 2 is processed at the end of the experiment, the average number of times per minute that the experimental fish in the transparent simulation tank 1 enter the escape zone 10, the ratio of the swimming speed of the experimental fish in the escape zone 10 to the swimming speed of the experimental fish in the detection area 6, the duration of each entry of the experimental fish into the escape zone 10, the percentage of the total time that the experimental fish stays in the escape zone 10 to the total time of the experiment, and the time taken by the experimental fish from the application of the stimulus to the first entry into the escape zone can be obtained.

[0038] In the embodiment of the present invention, the transparent simulation cylinder 1, the infrared night vision camera 2 and the water circulation device 3 are all arranged in a sealed chamber or a vacuum chamber. The transparent simulation cylinder 1 is a sealed test box spliced by transparent acrylic plates or organic glass, and the size of the transparent simulation cylinder 1 is 510mm×100mm×420mm; a water inlet 8 is opened at the bottom of the left cylinder wall of the transparent simulation cylinder 1, a water outlet 9 is opened at the top of the right cylinder wall of the transparent simulation cylinder 1, and a drain port 30 is opened at the bottom of the right cylinder wall of the transparent simulation cylinder 1. The drain port 30 is opened to facilitate faster replacement of the water in the transparent simulation cylinder 1 when changing a single variable of the experiment; each of the partitions 4 is made of a transparent acrylic plate or organic glass with the same width as that of the transparent simulation cylinder 1. In the embodiment of the present invention, the transparent simulation cylinder 1 is a sealed test box spliced by transparent acrylic plates or organic glass, and the size of the transparent simulation cylinder 1 is 510mm×100mm×420mm; a water inlet 8 is opened at the bottom of the left cylinder wall of the transparent simulation cylinder 1, a water outlet 9 is opened at the top of the right cylinder wall of the transparent simulation cylinder 1, and a drain port 30 is opened at the bottom of the right cylinder wall of the transparent simulation cylinder 1. The drain port 30 is opened to facilitate faster replacement of the water in the transparent simulation cylinder 1 when changing a single variable of the experiment; each of the partitions 4 is made of a transparent acrylic plate or organic glass with the same width as that of the transparent simulation cylinder 1. In the embodiment, the transparent simulation cylinder 1 and the partition 4 are both made of organic glass; sieve holes 31 are provided on each partition 4 corresponding to the water inlet 8, the water outlet 9 and the drain port 30 respectively, and the sieve holes 31 can ensure that the water flowing into the transparent simulation cylinder 1 from the water inlet 8 flows out from the water outlet 9 or the drain port 30; the water circulation device 3 is fixed on the top plate of the transparent simulation cylinder 1, and the water outlet end of the water circulation device 3 is connected to the water inlet area 5 through the water inlet pipe 33, and the water inlet end of the water circulation device 3 is connected to the water outlet area 7 through the water outlet pipe 32; the infrared night vision camera 2 is selected as model RS-217-4mm, and the infrared night vision camera 2 is connected to a computer, and the computer stores the image pictures collected by the infrared night vision camera 2 and performs digital processing.

[0039] An escape zone 10 is provided on one side of the monitoring area 6 close to the water outlet area 7, and the escape zone 10 is surrounded by a number of barrier columns 11 and partitions 4, and the distance between two adjacent barrier columns 11 is 1.0-2.0 cm; the volume of the escape zone 10 is 1 / 24 to 1 / 3 of the volume of the monitoring area 6; in the present invention, in a single standard test, the material of the barrier column 11 is selected from any one of a 0.6 cm diameter straw, a 2 mm diameter wooden stick, a 0.9 mm thick rubber band or a 0.7 mm diameter iron wire; but it should be clear that the materials that can be used for the barrier column 11 are not limited to the above four types, but can be replaced according to specific experimental requirements. Therefore, as long as the method of studying the biological behavior of experimental fish by replacing the material of the barrier column 11 to study the escape zone 10 composed of different materials should fall within the scope of protection of the present invention.

[0040] At the same time, it should be emphasized that the present invention selects straws, wooden sticks, rubber bands and iron wires in the embodiments because these materials have characteristics with strong differences, and they are not the materials for making structural escape shelters in the protection zone after obtaining the experimental results. The purpose of the experiment is to explore the characteristics of the materials of the structural shelters that the experimental fish like; the material of the structural escape shelter selected after the experiment should include all the beneficial characteristics of the experimental results.

[0041] like Figure 4 As shown, a number of lighting devices 12 are provided at the intersection of the escape zone 10 and the monitoring zone 6, each of the lighting devices 12 includes a light source device 22, a color-changing device 23 and a dimming knob 24, the color-changing device 23 is arranged along the lighting direction of the light source device 22, and the light-transmitting area of the color-changing device 23 covers the light-emitting end of the light source device 22, and the dimming knob 24 is electrically connected between the light source device 22 and the power supply device; the dimming knob 24 can adjust the current input into the light source device 22, and the light-emitting end of the light source device 22 irradiates different colors of light into the monitoring zone 6 through the light-transmitting area of the color-changing device 23; a light intensity meter can also be optionally provided, and the light intensity meter is electrically connected to the dimming knob 24, and the light intensity meter can detect the light intensity emitted by the light source device 22, and control the light intensity emitted by the light source device 22 through the dimming knob 24 according to the light intensity value set in advance.

[0042] like Figure 1 As shown, it also includes a backlight device, which is arranged on the outer side of the cylinder wall of the transparent simulation cylinder 1 away from the infrared night vision camera 2, that is, Figure 1 As shown in the A tank wall, the backlight device simulates the lighting conditions in the water under natural light during the experiment. Generally, a plurality of LED lighting lamps are distributed in a rectangular array on the A tank wall.

[0043] According to the experimental requirements, a sound generating device and a sound wave device can be set on the outer side of the cylinder wall of the transparent simulation cylinder 1 away from the infrared night vision camera 2, that is, Figure 1 As shown in the A cylinder wall, the sound-generating device can simulate the sound factors of the living waters of the experimental fish, and the sound wave device simulates ultrasonic or infrasonic waves. The device is mainly used for the establishment of marine fish protection areas. By the influence of the ultrasonic or infrasonic waves generated by the sound wave device on the experimental fish, the sound wave range in which the experimental fish can be unaffected can be determined, so that the material of the barrier column 11 that can meet the absorption requirements can be selected according to the sound wave range. Since they are all existing devices, the present invention will not be repeated here.

[0044] A pollutant supply device 13 is provided in the area of the water inlet area 5 near the top of the transparent simulation cylinder 1. The discharge end of the pollutant supply device 13 is connected to the monitoring area 6 through the partition 4 and discharges pollutants into the monitoring area 6; the pollutants in the pollutant supply device 13 can be selected from one or more of persistent organic pollutants (such as polycyclic aromatic hydrocarbons, pesticides, etc.), endocrine disruptors (such as biological feces, aquatic plant debris), antibiotics, trace sediment, heavy metal pollutants (such as lead, mercury, cadmium, etc.), microplastics, etc.; in the embodiment of the present invention, the pollutants in the pollutant supply device 13 are selected from endocrine disruptors, trace sediment and Cd-containing 2+The three heavy metal pollutants are mixed; among them, endocrine disruptors and trace sediments are used to adjust the turbidity content in the water environment of the transparent simulation tank 1; so that the water environment in the transparent simulation tank 1 is closer to the natural environment where the experimental fish live; Cd 2+ The heavy metal pollutants are separately added as stimulating agents during the experiment; therefore, the raw material storage device of the pollutant supply device 13 selected in the present invention preferably uses a storage device with multiple independent material boxes. Since the above-mentioned equipment exists in the prior art, it will not be described again; so that the selected pollutants can be discharged separately into the transparent simulation cylinder 1.

[0045] like Figure 5 As shown, the water outlet 9 is located on one side of the water outlet area 7 and is provided with a turbidity detection and filtering unit 14. The turbidity detection and filtering unit 14 includes a turbidity meter 15, a filtering device 16, a first flow pipe 17, and a second flow pipe 18. The turbidity meter 15 is arranged around the water outlet 9, and the filtering device 16 is arranged on the second flow pipe 18. The inflow end of the first flow pipe 17 and the inflow end of the second flow pipe 18 are respectively provided with a first solenoid valve 19 and a second solenoid valve 20, and the outflow end of the first flow pipe 17 and the outflow end of the second flow pipe 18 are both connected to the water inlet end of the water circulation device 3 through the water outlet 9; the turbidity meter 15 can monitor the turbidity of the water in the transparent water tank 1 in real time and control the connection or closing of the first solenoid valve 19 and the second solenoid valve 20 respectively, and at the same time, only one of the first solenoid valve 19 and the second solenoid valve 20 is in a connected state; the discharge end of the pollutant supply device 13 is provided with a third solenoid valve 21, and the turbidity meter 15 can control the connection or closing of the third solenoid valve 21.

[0046] In the process of adjusting the turbidity content of the water environment in the transparent simulation cylinder 1, a preset turbidity is first set on the turbidimeter 15, and then the third solenoid valve 21 is in a connected state, so that the endocrine disruptors and trace sediment in the pollutant supply device 13 enter the transparent simulation cylinder 1 to increase the turbidity of the water, and the turbidity meter 15 is started to detect the turbidity of the water in the transparent simulation cylinder 1. If the turbidity of the water in the transparent simulation cylinder 1 is less than the preset value of the turbidity meter 15, the turbidity meter 15 simultaneously controls the second solenoid valve 20 and the third solenoid valve 21 to be in a connected state, and controls the first solenoid valve 19 to be in a closed state. During this process, the filtering device 16 prevents turbid impurities in the water in the transparent simulation cylinder 1 from flowing out of the transparent simulation cylinder 1; if the turbidity of the water in the transparent simulation cylinder 1 is less than the preset value of the turbidity meter 15, the turbidity meter 15 simultaneously controls the second solenoid valve 20 and the third solenoid valve 21 to be in a connected state, and controls the first solenoid valve 19 to be in a closed state. During this process, the filtering device 16 prevents turbid impurities in the water in the transparent simulation cylinder 1 from flowing out of the transparent simulation cylinder 1; The turbidity of the water in the transparent simulation cylinder 1 is equal to the preset value of the turbidity meter 15, and the second solenoid valve 20 and the third solenoid valve 21 are controlled to be in a closed state, and the first solenoid valve 19 is controlled to be in a connected state. During this process, the turbid impurities in the water in the transparent simulation cylinder 1 flow in the transparent simulation cylinder 1 along with the circulating water flow generated by the water circulation device 3; if the turbidity of the water in the transparent simulation cylinder 1 is greater than the preset value of the turbidity meter 15, the second solenoid valve 20 and the third solenoid valve 21 are controlled to be in a closed state, and the first solenoid valve 19 is controlled to be in a connected state. During this process, the excess turbid impurities in the water in the transparent simulation cylinder 1 first enter the water circulation device 3, and then are discharged through the sewage discharge device connected to the water circulation device 3.

[0047] like Figure 2 As shown, the sewage discharge device includes a sewage pipe 34 and a normally closed fourth solenoid valve 35. The turbidity inlet end of the sewage pipe 34 is connected to the water inlet pipe 33. The fourth solenoid valve 35 is arranged at the sewage discharge end of the sewage pipe 34. The turbidity meter 15 can control the connection or closing of the fourth solenoid valve 35. A filter barrel can be arranged below the sewage pipe 34. After the turbidity-containing water discharged from the sewage pipe 34 is filtered by the filter barrel, it re-enters the water circulation device 3 through the connecting pipe and then re-enters the transparent simulation tank 1 through the water inlet pipe 33. This prevents the water in the transparent simulation tank 1 from being excessively reduced due to sewage discharge.

[0048] Since the purpose of the present invention is to explore the escape zone 10 selection of experimental fish in a nature reserve, that is, to explore the choice of endangered fish for the escape zone 10, compared with the existing experiments that use fish evasive biological behavior to detect water quality, when adding agents, a solution containing heavy metal ions cannot be directly added, so as to avoid excessive heavy metal content in the water in a short period of time causing excessive stimulation to the experimental fish, thereby avoiding irreversible damage to the experimental fish due to excessive stimulation. Therefore, the present invention uses a metal material that can precipitate metal ions in water to replace the heavy metal ion solution in the prior art, and the heavy metal pollutants will not move under the action of water flow after being put into the transparent fish tank 1, thereby forming a stable pollution source in the transparent fish tank 1; and since the heavy metals precipitate metal ions in the water, the metal ions The experimental process of the present invention takes a long time, so compared with the existing experimental process of using fish evasive biological behavior to detect water quality, which only takes a few minutes to more than ten minutes, the experimental process of the present invention is as long as dozens or even hundreds of hours; and in the experimental process of the present invention, the several experimental fish located in the transparent simulation tank 1 will inevitably produce endocrine disruptors due to metabolism, thereby causing the turbidity in the transparent simulation tank 1 to increase, so it is necessary to use the turbidity meter 15 to detect the turbidity of the water in the transparent simulation tank 1 at all times during the experiment. If the turbidity of the water in the transparent simulation tank 1 is greater than the preset value of the turbidity meter 15, the turbidity meter 15 controls the fourth solenoid valve 35 to change from a normally closed state to a connected state; and discharges excess turbidity impurities in the water in the transparent simulation tank 1 through the sewage pipe 34.

[0049] The partition 4 and the bottom of the transparent simulation cylinder 1 together enclose a sealed temperature control area 25, and a temperature controller 26 is provided in the temperature control area 25. The temperature controller 26 can heat the water in the transparent simulation cylinder 1; the temperature controller 26 is a heating pad, and the temperature control end of the temperature controller 26 is attached to the partition 4 opposite to the bottom of the monitoring area 6, so that the temperature controller 26 can evenly heat the water in the transparent simulation cylinder 1 to avoid the different water temperatures in different areas of the monitoring area 6 affecting the experimental results.

[0050] The water inlet 8 is located on one side of the water inlet area 5 and is provided with a residual chlorine detection and adjustment device 27 . The residual chlorine detection and adjustment device 27 can detect and adjust the residual chlorine content in the water body of the transparent simulation tank 1 .

[0051] It also includes a dissolved oxygen detection and adjustment device 28. The partition 4 and the top of the transparent simulation tank 1 together enclose an oxygen control area 29. The dissolved oxygen detection and adjustment device 28 is arranged in the oxygen control area 29, and the detection end and the oxygen supply end of the dissolved oxygen detection and adjustment device 28 both pass through the partition 4 and extend into the monitoring area 6. The dissolved oxygen detection and adjustment device 28 can detect and adjust the dissolved oxygen content in the water body of the transparent simulation tank 1.

[0052] The dissolved oxygen detection and regulation device 28 uses fiber optic sensing technology at the detection end to prevent electrode interference with the water, while the oxygen supply end uses nano-aerators to achieve silent oxygenation. This allows for experiments lasting hundreds of hours, preventing vibrations from traditional air pumps that might startle the experimental fish while also achieving a periodic gradient of dissolved oxygen concentration in the water, simulating the natural rhythms of tides or aquatic plant photosynthesis. Combined with the steady-state regulation capabilities of the temperature control zone 25, the entire simulation device simulates an environment that approaches natural observation conditions.

[0053] The residual chlorine detection and adjustment device 27 and the dissolved oxygen detection and adjustment device 28 together form a dynamic balance network for the water within the transparent test chamber 1. The former monitors the residual chlorine concentration in the water inlet area 5 in real time, automatically triggering a neutralization reaction mechanism to effectively eliminate potential damage to fish gill tissue caused by excess residual chlorine in the water. The latter, leveraging the enclosed structure of the transparent simulation tank 1, employs microbubble diffusion technology to precisely control dissolved oxygen levels in the water, simulating both oxygen-rich coral reef environments and the hypoxic characteristics of estuaries. Both systems support pre-set threshold linkage. When water quality parameters in the monitoring area 6 deviate from the set range, a compensation process is automatically initiated, ensuring that the experimental fish remain in a water environment consistent with their natural habits.

[0054] A method for simulating a shelter structure simulation device based on fish behavior includes the following steps:

[0055] Step 1: Establish an area determination experiment and determine the area where the escape zone 10 is built within the monitoring area 6 in the transparent simulation tank 1;

[0056] Step 2: Based on step 1, an escape zone 10 is constructed in the monitoring zone 6 in the transparent simulation cylinder 1;

[0057] Step 3: Pour water into the transparent simulation tank 1;

[0058] Step 4: Regulate the residual chlorine parameter in the water body through the residual chlorine detection and adjustment device 27; regulate the water temperature through the thermostat 26; and regulate the dissolved oxygen content in the water body through the dissolved oxygen detection and adjustment device 28;

[0059] Step 5: The turbidity meter 15 controls the third solenoid valve 21 on the discharge end of the pollutant supply device 13 to be in a connected state, so that the pollutant supply device 13 is in a connected state with the monitoring area 6, and non-toxic endocrine disruptors and sediment are released into the monitoring area 6;

[0060] Step 6: Turn on the light source device 22, and the turbidity meter 15 detects the turbidity in the water under the action of light, and controls the first solenoid valve 19 to be in a connected state or the second solenoid valve 20 to be in a connected state according to the detection result;

[0061] Step 7: Connect the water inlet of the water circulation device 3 to the water outlet 11 of the transparent simulation tank 1, connect the water outlet of the water circulation device 10 to the water inlet 12 of the transparent simulation tank 1, and control the water flow in the transparent simulation tank 1 through the water circulation device 10; thereby completing the preset standard test water environment;

[0062] Step 8: Based on step 7, place several experimental fish into the transparent simulation tank 1 at a male-to-female ratio of 1:1;

[0063] Step nine: starting the infrared night vision camera 2 so that the infrared night vision camera 2 can monitor and record the behavior of the experimental fish in the transparent simulation tank 1 in the water body;

[0064] Step 10: Use the color changing device 23 to change the color of the light irradiated by the light source device 22 into the monitoring area 7 to explore the biological behavior of the experimental fish under different light colors; under the same light color, use the dimming knob 24 to adjust the luminous intensity of the light source device 22;

[0065] Step 11: Change the volume of the escape zone 10 to explore the biological behavior of the experimental fish under different escape zone volumes.

[0066] Step 12: Change the material of the obstacle column 11 that constitutes the escape zone 10 to explore the biological behavior of the experimental fish under different obstacle column 11 materials

[0067] Step 13: Conduct comparative experimental analysis, including:

[0068] a) Each independent experiment is allowed to change only a single variable condition in step 10, step 11 or step 12;

[0069] b) When executing step 10, step 11, or step 12, heavy metal pollutants need to be added to the transparent simulation tank 1 through the pollutant supply device 13 at the same interval after the experimental fish is placed in the transparent simulation tank 1; thereby stimulating the experimental fish. Of course, the pollutants discharged here do not necessarily have to be heavy metal pollutants, as long as any substance that can stimulate the experimental fish can be added at this time;

[0070] c) When executing step 10, step 11 or step 12, it is necessary to re-execute steps 1 to 8 to re-establish the standard test environment;

[0071] Among them, during the experimental process of determining the area where the escape zone 10 is located in step one, it is necessary to complete the entire process from step three to step nine, and after a period of time after the experimental fish is placed in the transparent simulation tank 1, heavy metal pollutants are added to the transparent simulation tank 1 through the pollutant supply device 13; the interval time of step one is the same as the interval time in step thirteen in the subsequent formal test.

[0072] Among them, the various devices in steps four to seven cooperate with each other to form a standard test water environment that should be the same as the most suitable environment for the experimental fish to live in nature; the specific values require sampling the water where the experimental fish live in nature before the experiment, determining the flow rate of the water where the experimental fish live in nature, and the light intensity in the water during sampling, and analyzing the element types and contents of the sample water after sampling.

[0073] Among them, by changing the single variable in step 10, the favorite light color and appropriate light intensity of the experimental fish can be explored, so that when the escape structure is subsequently manufactured, a light-emitting device can be installed on the escape structure, so that the experimental fish can gather near the escape structure in the protection zone, thereby increasing the survival rate of the experimental fish in the protection zone and making it easier for staff to feed them adaptively according to the light;

[0074] In the theoretical verification test, zebrafish was selected as the experimental fish, and the standard test water environment was as follows: the water flow in the transparent simulation tank 1 was circulated at a speed of 1 L / min through the water circulation device 3; the residual chlorine parameter in the water in the transparent simulation tank 1 was made less than 0.03 mg / L through the residual chlorine detection and adjustment device 27; the water temperature in the fish tank 1 was maintained at 22-26°C through the thermostat 26, with 25°C being optimal; the turbidity in the water in the fish tank 1 was made less than 10 NTU through the turbidity detection and filtration unit 14; the dissolved oxygen in the water in the fish tank 1 was made greater than 6 mg / L through the dissolved oxygen detection and adjustment device 28; the backlight device was adjusted to continuously emit light at a light intensity of 50 lx; it should be emphasized that the parameters of the standard test water environment are only specific values after testing the water in which the experimental fish live in nature, but in the actual environment, the water environment in which the experimental fish live is not static, so during the experiment, all parameters should undergo periodic gradient changes within a certain range.

[0075] After establishing the standard test water environment, the monitoring area 6 is first divided into four areas: upper left, lower left, upper right and lower right. Then, the data is processed through step 1 and the following is obtained. Figure 6 The experimental results shown in the figure show that zebrafish prefer to escape in the lower right area of the monitoring area 6. The reason is that the lower right area is located below and downstream of the flowing water in the transparent simulation tank 1 and is also the farthest from the pollutant supply device 13 (i.e., the pollution source). Therefore, the zebrafish will swim to the lower right area autonomously after being stimulated. Therefore, in subsequent experiments, the escape area 10 is chosen to be built in the lower right area of the monitoring area 6.

[0076] By changing the single variable experiment in step 10 and processing the data, we can obtain the following Figure 7The experimental results shown are due to the fact that red light stimulation significantly improves the sensitivity of zebrafish by specifically activating the L-cone pathway of zebrafish; thus, it enables the zebrafish to pass through the gaps between the barrier pillars 11 and enter the escape zone 10;

[0077] By changing the single variable experiment in step 11 and processing the data, we can obtain the following Figure 8 The experimental results shown in the figure show that when the volume of the escape zone 10 is 1 / 3 of the volume of the monitoring zone 6, the escape zone 10 can no longer represent the escape area of fish in nature. Therefore, the 1 / 3 curve needs to be removed when analyzing the results. After removing the 1 / 3 curve, the optimal volume of the escape zone 10 is 1 / 12 of the volume of the monitoring zone 6.

[0078] By changing the single variable experiment in step 12 and processing the data, we can obtain the following Figure 9 The experimental results shown in the figure show that the curve shown by the elastic material represented by the rubber band is formed because, initially, without the action of external force, the zebrafish quickly swims to the escape zone 10 after being stimulated. However, during the escape process, if the zebrafish hits the rubber band, the rubber band will cause continuous vibration or swinging due to its own elasticity, which will panic the zebrafish and cause it to escape from the escape zone 10. The curve shown by the absorptive material represented by the wooden stick is formed because wooden products placed directly into the water without professional treatment will emit special chemicals in the surrounding area, causing the zebrafish to be reluctant to swim to the escape zone 10 even without the addition of external stimulation. However, when the external stimulation of the pollution source is added, the pollutants in the water are absorbed around the wooden stick due to its own adsorption effect, causing the zebrafish to escape from the escape zone 10. The curve shown by the corrosive material represented by the iron wire is formed because when the iron wire is left in water for a long time, its surface will oxidize and rust, causing the rust to spread into the water, reducing the water quality in the escape zone 10, and causing the zebrafish to escape from the escape zone 10.

[0079] Moreover, the present invention also found that the surface of the straw and the surface of the rubber band in the taut state are smoother than the surface of the steel wire and the surface of the wooden stick, and the reason why zebrafish prefer the rubber band before the administration of the drug is that the material of the rubber band is softer than the straw and closer to the aquatic plants in nature; therefore, when finally choosing the material for manufacturing the structural escape shelter of the protected area, a material that is relatively soft and can swing with the water flow, has a relatively smooth surface, has no adsorption and corrosiveness, and is not easy to deform when subjected to external force should be selected; of course, this result is only limited to the relatively few material choices proposed by the present invention. In the actual simulation process, the types of materials available for experiment can be further increased, so that the final structural shelter can be liked by endangered fish.

[0080] The above is the content of the embodiment of the present invention. The content recorded in the specification does not represent all the effects that can be achieved by the simulation device of the present invention. For the convenience of analysis, the specification only records the impact of changing a single variable on the behavior of the experimental fish. The experimental results also only show the result combination after the comparison of a single variable. However, in actual application, the escape behavior of fish after being stimulated is affected by multiple factors, not just a single factor. The water environment also has multiple parameters that change at the same time at all times. Therefore, in actual simulation experiments, multiple variables should be changed at the same time to make the research results more accurate.

[0081] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A shelter structure simulation device based on fish behavior, characterized by: The invention comprises a transparent simulation cylinder (1), an infrared night vision camera (2) and a water circulation device (3). The interior of the transparent simulation cylinder (1) is divided into a water inlet area (5), a monitoring area (6) and a water outlet area (7) from left to right by a plurality of crisscross partitions (4); an escape area (10) is provided in the monitoring area (6); a water inlet (8) is provided on the cylinder wall of the water inlet area (5) near the bottom area of the transparent simulation cylinder (1); a water outlet (9) is provided on the cylinder wall of the water outlet area (7) near the top area of the transparent simulation cylinder (1); the water circulation device (3) is located outside the transparent simulation cylinder (1), and the water outlet end of the water circulation device (3) is connected to the water inlet area (5) through the water inlet (8). ) is connected, and the water inlet end of the water circulation device (3) is connected to the water outlet area (7) through the water outlet (9); the infrared night vision camera (2) is arranged on the periphery of the transparent simulation tank (1), and the camera head of the infrared night vision camera (2) is facing the detection area (6); the infrared night vision camera (2) can monitor and record the average number of times per minute that the experimental fish in the transparent simulation tank (1) enter the escape area (10), the swimming speed of the experimental fish in the escape area (10) and the swimming speed of the experimental fish in the detection area (6), the duration of each entry of the experimental fish into the escape area (10), the total time the experimental fish stays in the escape area (10) and the time taken by the experimental fish from the application of stimulation to the first entry into the escape area.

2. The fish behavior-based shelter structure simulation device according to claim 1, characterized in that: The escape zone (10) is located on one side of the monitoring zone (6) close to the water outlet zone (7). The escape zone (10) is surrounded by a plurality of barrier columns (11) and partitions (4). The volume of the escape zone (10) is 1 / 24 to 1 / 3 of the volume of the monitoring zone (6).

3. The fish behavior-based shelter structure simulation device according to claim 2, characterized in that: A plurality of illumination devices (12) are provided at the intersection of the escape zone (10) and the monitoring zone (6), each illumination device (12) comprising a light source device (22), a color changing device (23) and a dimming knob (24), the color changing device (23) being provided along the illumination direction of the light source device (22), and the light-transmitting area of the color changing device (23) covering the light-emitting end of the light source device (22), the dimming knob (24) being electrically connected between the light source device (22) and the power supply device; the dimming knob (24) being capable of adjusting the magnitude of the current input into the light source device (22), and the light-emitting end of the light source device (22) irradiating light of different colors into the monitoring zone (6) through the light-transmitting area of the color changing device (23).

4. The fish behavior-based shelter structure simulation device according to claim 1, characterized in that: A pollutant supply device (13) is provided in the area of the water inlet area (5) near the top of the transparent simulation cylinder (1), and the discharge end of the pollutant supply device (13) passes through the partition (4) and is connected to the monitoring area (6), and discharges pollutants into the monitoring area (6).

5. The fish behavior-based shelter structure simulation device according to claim 1, characterized in that: The water outlet (9) is located on one side of the water outlet area (7) and is provided with a turbidity detection and filtering unit (14). The turbidity detection and filtering unit (14) comprises a turbidity meter (15), a filtering device (16), a first flow pipe (17), and a second flow pipe (18). The turbidity meter (15) is arranged around the water outlet (9), the filtering device (16) is arranged on the second flow pipe (18), and the inflow end of the first flow pipe (17) and the inflow end of the second flow pipe (18) are respectively provided with a first electromagnetic The first solenoid valve (19) and the second solenoid valve (20) are connected, and the outflow end of the first circulation pipe (17) and the outflow end of the second circulation pipe (18) are both connected to the water inlet end of the water circulation device (3) through the water outlet (9); the turbidity meter (15) can monitor the turbidity in the water body of the transparent water tank (1) in real time, and respectively control the connection or closing of the first solenoid valve (19) and the second solenoid valve (20), and at the same time, only one of the first solenoid valve (19) and the second solenoid valve (20) is in a connected state.

6. The fish behavior-based shelter structure simulation device according to claim 5, characterized in that: A third electromagnetic valve (21) is provided at the discharge end of the pollutant supply device (13), and the turbidity meter (15) can control the connection or closing of the third electromagnetic valve (21).

7. The fish behavior-based shelter structure simulation device according to claim 1, characterized in that: The partition (4) and the bottom of the transparent simulation tank (1) together enclose a sealed temperature control area (25), and a temperature controller (26) is provided in the temperature control area (25). The temperature controller (26) can heat the water in the transparent simulation tank (1).

8. The fish behavior-based shelter structure simulation device according to claim 1, characterized in that: A residual chlorine detection and adjustment device (27) is provided on one side of the water inlet (8) located in the water inlet area (5). The residual chlorine detection and adjustment device (27) can detect and adjust the residual chlorine content in the water body of the transparent simulation tank (1).

9. The fish behavior-based shelter structure simulation device according to claim 1, characterized in that: The invention also includes a dissolved oxygen detection and adjustment device (28). The partition (4) and the top of the transparent simulation tank (1) together enclose an oxygen control area (29). The dissolved oxygen detection and adjustment device (28) is arranged in the oxygen control area (29), and the detection end and the oxygen supply end of the dissolved oxygen detection and adjustment device (28) both pass through the partition (4) and extend into the monitoring area (6). The dissolved oxygen detection and adjustment device (28) can detect and adjust the dissolved oxygen content in the water body of the transparent simulation tank (1).

10. The method for simulating a shelter structure simulation device based on fish behavior according to claim 9, characterized in that: The following steps are involved: Step 1: Building an escape zone (10) within the monitoring zone (6) in the transparent simulation cylinder (1); Step 2: injecting water into the transparent simulation tank (1); Step 3: regulating the residual chlorine parameter in the water body through the residual chlorine detection and adjustment device (27); regulating the water temperature through the thermostat (26); regulating the dissolved oxygen content in the water body through the dissolved oxygen detection and adjustment device (28); the turbidity meter (15) controls the third electromagnetic valve (21) on the discharge end of the pollutant supply device (13) to be in a connected state, so that the pollutant supply device (13) is in a connected state with the monitoring area (6), and non-toxic endocrine disruptors and sediment are added into the monitoring area (6); turning on the light source device (22), the The turbidity meter (15) detects the turbidity in the water body under the action of light, and controls the first electromagnetic valve (19) to be in a connected state or the second electromagnetic valve (20) to be in a connected state according to the detection result; the water inlet end of the water circulation device (3) is connected to the water outlet (11) of the transparent simulation cylinder (1), the water outlet end of the water circulation device (10) is connected to the water inlet (12) of the transparent simulation cylinder (1), and the water flow rate in the transparent simulation cylinder (1) is controlled by the water circulation device (10); thereby completing the preset of the standard test environment; Step 4: Based on step 3, several experimental fish are placed into the transparent simulation tank (1) at a male-to-female ratio of 1:1; Step 5: starting the infrared night vision camera (2) so that the infrared night vision camera (2) can monitor and record the behavior of the experimental fish in the transparent simulation tank (1) in the water body; Step 6: changing the color of the light emitted by the light source device (22) to the monitoring area (7) through the color changing device (23); adjusting the luminous intensity of the light source device (22) through the dimming knob (24) under the same light color; Step 7: changing the volume of the escape zone (10); Step 8: Changing the material of the obstruction column (11) constituting the escape zone (10); Step 9: Conduct comparative experimental analysis, including: a) Each independent experiment is allowed to change only a single variable condition in step 6, step 7 or step 8; b) When executing step 6, step 7 or step 8, it is necessary to add toxic heavy metal pollutants into the transparent simulation tank (1) through the pollutant supply device (13) at the same interval as after the experimental fish is placed into the transparent simulation tank (1); c) When executing step 6, step 7 or step 8, it is necessary to re-execute steps 1 to 4 to re-establish the standard test environment.

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