Whitening and anti-wrinkle protein active peptide composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of active peptides, and discloses a whitening and anti-wrinkle protein active peptide composition as well as a preparation method and application thereof. The whitening and anti-wrinkle protein active peptide composition is prepared from the following raw materials in parts by weight: 10 to 15 parts of elastin peptide, 11 to 14 parts of fish collagen peptide, 8 to 10 parts of fish maw small-molecule active peptide, 5 to 10 parts of cubilose peptide, 5 to 10 parts of sea cucumber peptide, 2 to 6 parts of Chinese fevervine herb active peptide, 1 to 3 parts of zingiber corianum active peptide and 1 to 5 parts of lycopene. According to the whitening and anti-wrinkle protein active peptide composition and the preparation method and application thereof, small-molecule active peptide in the prepared protein active peptide composition is beneficial to absorption of dermal cells, and meanwhile, through the synergistic effect of multiple active ingredients, the whitening and anti-wrinkle effects are effectively achieved, the skin quality is improved, and aging is delayed.

Description

Technical Field

[0001] The present invention relates to the technical field of active peptides, in particular to a whitening and anti-wrinkle protein active peptide composition, a preparation method and an application thereof. Background Art

[0002] With rapid economic development and improved living standards, people are paying more attention to skin care. Appearance plays a crucial role in personal interactions, both in personal and professional settings. Skin color is formed through a complex process involving the skin's physiological surface conditions, blood flow, moisture, pigmentation, and other components. Melanin is the primary determinant of skin color. Melanin in the human body is a complex combination of insoluble eumelanin and soluble pheomelanin. Genetic factors, reactive oxygen species, endocrine disorders, UV damage, and abnormal levels of trace elements in the body can accelerate melanin production.

[0003] Melanin deposition may accompany or accompany the development of wrinkles. Wrinkles are the result of a combination of natural aging and photoaging. The root cause of wrinkle formation is changes in the dermis induced by exposure to medium-wavelength ultraviolet light (290-320nm). The dermal extracellular matrix is composed of a network of collagen and elastin fibers, interspersed with matrix substances such as proteoglycans (GAGs). Collagen is the most abundant protein in the skin and its primary structural protein. Studies have shown that exposure to ultraviolet light reduces collagen content, causing the skin to become stiff and lose its elasticity. Elastin is also a key protein in the skin, accounting for 1% to 2% of the skin's dry weight and contributing to its elasticity and suppleness. Ultraviolet light exposure can denature and damage elastin in the skin, depriving it of its original function. Furthermore, ultraviolet light exposure triggers cellular metabolism in the body, generating oxygen free radicals. On the one hand, free radicals can cause lipid peroxidation, DNA damage and protein denaturation. On the other hand, they can upregulate the expression of genes such as elastin and matrix metalloproteinases (MMPs) and downregulate the expression of collagen, causing wrinkles and skin sagging.

[0004] Active peptides refer to peptides that perform special functions in organisms and are substances between amino acids and proteins. Active peptides often act as messengers to transmit chemical signals within or between cells, regulating the actions between cells or organs. Scientific research has found that neurological, immune, and aging effects are all related to active peptides. Active peptides have anti-aging, whitening, antioxidant, anti-glycation, soothing, repairing, and antibacterial effects. Therefore, the present invention provides a whitening and anti-wrinkle protein active peptide composition that can brighten the skin tone and reduce the formation of wrinkles. Summary of the Invention

[0005] The purpose of the present invention is to provide a whitening and anti-wrinkle protein active peptide composition and its preparation method and application. The small molecule active peptides in the prepared protein active peptide composition are conducive to the absorption of dermal cells. At the same time, through the synergistic effect of multiple active ingredients, it effectively whitens and anti-wrinkles, improves skin quality, and delays aging.

[0006] To achieve the above objectives, the present invention provides a whitening and anti-wrinkle protein active peptide composition, comprising the following raw materials in parts by weight: 10-15 parts of elastin peptide, 11-14 parts of fish collagen peptide, 8-10 parts of fish maw small molecule active peptide, 5-10 parts of bird's nest peptide, 5-10 parts of sea cucumber peptide, 2-6 parts of scutellaria baicalensis active peptide, 1-3 parts of Zingiber officinale active peptide, and 1-5 parts of lycopene, wherein the molecular weight of the elastin peptide, fish collagen peptide, fish maw small molecule active peptide, bird's nest peptide, sea cucumber peptide, scutellaria baicalensis active peptide, and Zingiber officinale active peptide is 200-2000 Daltons, and the molecular weight of the sea cucumber peptide is 200-500 Daltons.

[0007] Furthermore, a whitening and anti-wrinkle protein active peptide composition includes the following raw materials in parts by weight: 15 parts of elastin peptide, 13 parts of fish collagen peptide, 8 parts of fish maw small molecule active peptide, 6 parts of bird's nest peptide, 6 parts of sea cucumber peptide, 5 parts of Cistanche deserticola active peptide, 1 part of Zingiber officinale active peptide, and 4 parts of lycopene.

[0008] Furthermore, in step 1, the mass volume ratio (m / v) of fish to deionized water is 1:4; in step 2, the amount of glucose added is 3%, the water bath heating temperature is 120° C., and the water bath heating time is 100 min.

[0009] Furthermore, the preparation method of the active peptide of Patella dungareta is as follows:

[0010] Step 1: washing, drying and crushing the roots and stems of the Cistanche deserticola to obtain Cistanche deserticola powder;

[0011] Step 2: Add the Cercidiphyllum dungare powder to deionized water to prepare a suspension with a concentration of 10%, then add protease for enzymatic hydrolysis, inactivate the enzyme after enzymatic hydrolysis, filter, and filter the permeate through an ultrafiltration membrane with a molecular weight cutoff of 1000-2000Da to obtain the Cercidiphyllum dungare active peptide.

[0012] Furthermore, in step 2, during enzymatic hydrolysis, 2% alkaline protease is first added for enzymatic hydrolysis, and 5% flavor protease is added for enzymatic hydrolysis after 5 minutes; the enzymatic hydrolysis conditions are: enzymatic hydrolysis temperature 50° C., pH 6.4-7.7, and time 60 minutes.

[0013] Furthermore, the preparation method of the Zingiber officinale active peptide is as follows:

[0014] Step 1: washing, drying, crushing and detoxifying the leaves and stems of Zingiber officinale to obtain Zingiber officinale powder;

[0015] Step 2: adding Zingiber officinale powder to deionized water to prepare a suspension with a concentration of 8%, then adding protease for enzymatic hydrolysis, performing enzyme inactivation treatment after enzymatic hydrolysis, filtering, and filtering the permeate through an ultrafiltration membrane with a molecular weight cutoff of 1200-2000Da to obtain Zingiber officinale active peptide.

[0016] Furthermore, in step 2, during enzymatic hydrolysis, alkaline protease is first added for enzymatic hydrolysis, and then flavor protease is added for enzymatic hydrolysis; the enzymatic hydrolysis conditions of alkaline protease are: enzyme amount 2%, enzymatic hydrolysis temperature 50°C, pH 7.0, and time 75 minutes; the enzymatic hydrolysis conditions of flavor protease are: enzyme amount 4%, enzymatic hydrolysis temperature 53°C, pH 6.4, and time 50 minutes.

[0017] The present invention also discloses a preparation method of the above-mentioned whitening and anti-wrinkle protein active peptide composition, which comprises the following steps: weighing the freeze-dried powder of each component according to parts by weight, mixing them evenly, and obtaining the protein active peptide composition.

[0018] The present invention also discloses the use of the whitening and anti-wrinkle protein active peptide composition in the preparation of whitening and anti-wrinkle products.

[0019] The advantages and positive effects of the whitening and anti-wrinkle protein active peptide composition, its preparation method and application described in the present invention are:

[0020] The elastin peptide, fish collagen peptide, fish maw small molecule active peptide, and bird's nest peptide in the present invention can replenish the collagen in the dermis of the skin and fill damaged cells in the human body. At the same time, the epidermal growth factor (EGF) in the bird's nest peptide promotes the proliferation and repair of dermal cells, restores the roundness of shriveled cells, and thus reduces wrinkles; sea cucumber peptide, scutellaria baicalensis active peptide, and ginger active peptide have anti-inflammatory and antioxidant effects, reduce skin inflammation and free radical damage, soothe the skin, reduce wrinkles and spots, and at the same time, ginger active peptide inhibits tyrosinase activity, reduces melanin production, whitens the skin, and lightens spots; lycopene can neutralize free radicals, reduce photoaging, protect the skin from ultraviolet damage, and delay aging; therefore, the present invention effectively whitens and anti-wrinkles, improves skin quality, and delays aging through the synergistic effect of multiple active ingredients.

[0021] The molecular weights of the elastin peptide, fish collagen peptide, fish maw small molecule active peptide, bird's nest peptide, scutellaria baicalensis active peptide, and ginger active peptide in the present invention are 200-2000 Daltons, and the molecular weight of the sea cucumber peptide is 200-500 Daltons. Peptides with small molecular weight are more conducive to absorption and have better effects.

[0022] The technical solution of the present invention is further described in detail below through examples. DETAILED DESCRIPTION

[0023] The technical solutions of the present invention are further described below by way of examples. Unless otherwise defined, technical terms or scientific terms used in the present invention should have the same general meanings as those commonly understood by persons skilled in the art in the field to which the present invention belongs.

[0024] Unless otherwise defined or indicated, all technical and scientific terms used in this invention have the same meanings as those familiar to those skilled in the art. Furthermore, any methods and materials similar or equivalent to those described herein may be applied to the methods of the present invention. It should be noted that, unless there is a conflict, the embodiments and features of the embodiments of the present invention may be combined with each other.

[0025] The preparation method of the active peptide of Cercidiphyllum dungarum is as follows:

[0026] Step 1: washing, drying and crushing the roots and stems of the Cistanche deserticola to obtain Cistanche deserticola powder;

[0027] Step 2: adding the Patella dungaree powder to deionized water to prepare a suspension with a concentration of 10%, then adding protease for enzymatic hydrolysis, performing enzyme inactivation treatment after enzymatic hydrolysis, filtering, and filtering the permeate through an ultrafiltration membrane with a molecular weight cutoff of 1000-2000Da to obtain Patella dungaree active peptide;

[0028] During enzymatic hydrolysis, 2% alkaline protease was first added for enzymatic hydrolysis, and 5% flavor protease was added for enzymatic hydrolysis after 5 minutes. The enzymatic hydrolysis conditions were: enzymatic hydrolysis temperature 50° C., pH 6.5, and time 60 minutes.

[0029] The preparation method of Zingiber officinale active peptide is as follows:

[0030] Step 1: washing, drying, crushing and detoxifying the leaves and stems of Zingiber officinale to obtain Zingiber officinale powder;

[0031] Step 2: adding Zingiber officinale powder to deionized water to prepare a suspension with a concentration of 8%, then adding protease for enzymatic hydrolysis, performing enzyme inactivation treatment after enzymatic hydrolysis, filtering, and filtering the permeate through an ultrafiltration membrane with a molecular weight cutoff of 1200-2000Da to obtain Zingiber officinale active peptide;

[0032] During enzymatic hydrolysis, alkaline protease is first added for enzymatic hydrolysis, and then flavor protease is added for enzymatic hydrolysis; the enzymatic hydrolysis conditions of alkaline protease are: enzyme addition amount 2%, enzymatic hydrolysis temperature 50°C, pH 7.0, and time 75 minutes; the enzymatic hydrolysis conditions of flavor protease are: enzyme addition amount 4%, enzymatic hydrolysis temperature 53°C, pH 6.4, and time 50 minutes.

[0033] Example 1

[0034] A whitening and anti-wrinkle protein active peptide composition comprises the following raw materials in parts by weight:

[0035] 10 parts of elastin peptide, 11 parts of fish collagen peptide, 8 parts of fish maw small molecule active peptide, 5 parts of bird's nest peptide, 5 parts of sea cucumber peptide, 2 parts of Cistanche deserticola active peptide, 1 part of Zingiber officinale active peptide, and 1 part of lycopene.

[0036] Example 2

[0037] A whitening and anti-wrinkle protein active peptide composition comprises the following raw materials in parts by weight:

[0038] 15 parts of elastin peptide, 13 parts of fish collagen peptide, 8 parts of fish maw small molecule active peptide, 6 parts of bird's nest peptide, 6 parts of sea cucumber peptide, 5 parts of scutellaria baicalensis active peptide, 1 part of ginger active peptide, and 4 parts of lycopene.

[0039] Example 3

[0040] A whitening and anti-wrinkle protein active peptide composition comprises the following raw materials in parts by weight:

[0041] 15 parts of elastin peptide, 14 parts of fish collagen peptide, 10 parts of fish maw small molecule active peptide, 10 parts of bird's nest peptide, 10 parts of sea cucumber peptide, 6 parts of Cistanche deserticola active peptide, 3 parts of Zingiber officinale active peptide, and 5 parts of lycopene.

[0042] Comparative Example 1

[0043] A whitening and anti-wrinkle protein active peptide composition comprises the following raw materials in parts by weight:

[0044] 15 parts of elastin peptide, 13 parts of fish collagen peptide, 8 parts of fish maw small molecule active peptide, 6 parts of bird's nest peptide, 6 parts of sea cucumber peptide, 6 parts of corn active peptide, 4 parts of lycopene.

[0045] Comparative Example 2

[0046] A whitening and anti-wrinkle protein active peptide composition comprises the following raw materials in parts by weight:

[0047] 15 parts of elastin peptide, 19 parts of fish collagen peptide, 8 parts of fish maw small molecule active peptide, 6 parts of bird's nest peptide, 5 parts of Cistanche deserticola active peptide, 1 part of Zingiber officinale active peptide, and 4 parts of lycopene.

[0048] Performance measurement

[0049] Scavenging effect of DPPH free radicals:

[0050] Prepare a 2.0 μg / mL sample solution in anhydrous ethanol and a 0.04 mg / mL DPPH solution in anhydrous ethanol. Mix the DPPH solution and sample solution in a 3:1 volume ratio. Incubate at room temperature in the dark for 30 minutes and measure the absorbance at 517 nm. Three replicates were performed for each group. The DPPH free radical scavenging rate was calculated as follows:

[0051] DPPH free radical scavenging rate = [1-(A1-A2) / A0] × 100%.

[0052] Where: A0 is the absorbance of the blank group (DPPH solution + distilled water); A1 is the absorbance of the sample group (DPPH solution + sample solution); A2 is the absorbance of the control group (anhydrous ethanol + sample solution).

[0053] Scavenging effect of hydroxyl free radicals:

[0054] To 1 mL of sample solution, add 1 mL of 9 mmol / L FeSO4, 1 mL of 9 mmol / L salicylic acid-ethanol solution, and 1 mL of 8.8 mmol / L H2O2 in sequence. After oscillation and mixing, place in a water bath at 37°C for 30 min. Measure the absorbance at a wavelength of 510 nm. Set up 3 parallels for each group.

[0055] The hydroxyl radical scavenging rate was calculated as follows:

[0056] Hydroxyl radical scavenging rate = [1-(A1-A2) / A0] × 100%.

[0057] Where: A0 is the absorbance of the blank group (salicylic acid + distilled water); A1 is the absorbance of the sample group (salicylic acid + sample solution); A2 is the absorbance of the control group (distilled water + sample solution).

[0058] The results are shown in Table 1:

[0059] Table 1 Free radical scavenging rate

[0060]

[0061]

[0062] As shown in Table 1, the protein active peptide compositions prepared in Examples 1, 2, and 3 can effectively scavenge free radicals, reduce free radical damage, and reduce wrinkles. Compared with Example 2, the free radical scavenging rates of Comparative Examples 1 and 2 decreased, indicating that after adding sea cucumber peptides, scutellaria baicalensis active peptides, and ginger active peptides, the multiple ingredients synergize with each other, not only having anti-inflammatory and antioxidant effects, but also reducing skin inflammation and free radical damage, soothing the skin, and reducing wrinkles and spots.

[0063] Tyrosinase inhibition:

[0064] Using L-tyrosine as a substrate, tyrosinase oxidizes L-tyrosine to DOPA, which has a maximum absorption at 475 nm. The change in absorbance is used to determine the tyrosinase inhibition rate of the sample solution. The specific steps are as follows: Prepare a 0.1 mg / mL sample solution using 20% DMSO. The reaction system is shown in Table 2. Add 10 mmol / L L-tyrosine, the sample solution, and 0.067 mol / L phosphate buffer (pH 6.8) to a test tube in sequence. Preheat the solution in a 37°C water bath for 10 minutes. Then, add 200 U / mL of the enzyme solution, mix thoroughly, and maintain in a 37°C water bath for 15 minutes. Measure the absorbance at 475 nm and calculate the inhibition rate using the following formula:

[0065] Inhibition rate = [1-(A1-A2 / B1-B2)] x 100%.

[0066] Table 2 Reaction system

[0067]

[0068]

[0069] The results are shown in Table 3:

[0070] Table 3 Tyrosinase inhibition rate

[0071] Tyrosinase inhibition rate / % Example 1 81.1 Example 2 82.3 Example 3 80.4 Comparative Example 1 66.9 Comparative Example 2 71.8

[0072] As shown in Table 3, the protein active peptide compositions prepared in Examples 1, 2, and 3 can effectively inhibit the activity of tyrosinase, inhibit the formation of melanin, whiten the skin, and reduce spots. Compared with the examples, the inhibition rates of Comparative Examples 1 and 2 are lower, indicating that the various small molecule peptides in the present invention work synergistically with each other, not only having an antioxidant effect, but also inhibiting melanin production, brightening the skin tone, and whitening and anti-wrinkle.

[0073] The elastin peptide, fish collagen peptide, fish maw small molecule active peptide, and bird's nest peptide in the present invention can replenish the collagen in the dermis of the skin and fill damaged cells in the human body. At the same time, the epidermal growth factor (EGF) in the bird's nest peptide promotes the proliferation and repair of dermal cells, restores the roundness of shriveled cells, and thus reduces wrinkles; sea cucumber peptide, scutellaria baicalensis active peptide, and ginger active peptide have anti-inflammatory and antioxidant effects, reduce skin inflammation and free radical damage, soothe the skin, reduce wrinkles and spots, and at the same time, ginger active peptide inhibits tyrosinase activity, reduces melanin production, whitens the skin, and reduces spots; lycopene can neutralize free radicals, reduce photoaging, protect the skin from ultraviolet damage, and delay aging.

[0074] Therefore, the present invention adopts the above-mentioned whitening and anti-wrinkle protein active peptide composition and its preparation method and application. The small molecule active peptides in the prepared protein active peptide composition are conducive to the absorption of dermal cells. At the same time, through the synergistic effect of multiple active ingredients, it effectively whitens and anti-wrinkles, improves skin quality, and delays aging.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solutions of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A whitening and anti-wrinkle protein active peptide composition, characterized in that: The invention comprises the following raw materials in parts by weight: 10-15 parts of elastin peptide, 11-14 parts of fish collagen peptide, 8-10 parts of fish maw small molecule active peptide, 5-10 parts of bird's nest peptide, 5-10 parts of sea cucumber peptide, 2-6 parts of scutellaria baicalensis active peptide, 1-3 parts of zingiber officinale active peptide, and 1-5 parts of lycopene, wherein the molecular weight of the elastin peptide, fish collagen peptide, fish maw small molecule active peptide, bird's nest peptide, scutellaria baicalensis active peptide, and zingiber officinale active peptide is 200-2000 Daltons, and the molecular weight of the sea cucumber peptide is 200-500 Daltons.

2. A whitening and anti-wrinkle protein active peptide composition according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 15 parts of elastin peptide, 13 parts of fish collagen peptide, 8 parts of fish maw small molecule active peptide, 6 parts of bird's nest peptide, 6 parts of sea cucumber peptide, 5 parts of Cistanche deserticola active peptide, 1 part of Zingiber officinale active peptide and 4 parts of lycopene.

3. The whitening and anti-wrinkle protein active peptide composition and its preparation method and use according to claim 1, characterized in that: The preparation method of the active peptide of Cercidiphyllum dungarum is as follows: Step 1: washing, drying and crushing the roots and stems of the Cistanche deserticola to obtain Cistanche deserticola powder; Step 2: Add the Cercidiphyllum dungare powder to deionized water to prepare a suspension with a concentration of 10%, then add protease for enzymatic hydrolysis, inactivate the enzyme after enzymatic hydrolysis, filter, and filter the permeate through an ultrafiltration membrane with a molecular weight cutoff of 1000-2000Da to obtain the Cercidiphyllum dungare active peptide.

4. A whitening and anti-wrinkle protein active peptide composition according to claim 3, characterized in that: In step 2, during enzymatic hydrolysis, 2% alkaline protease is first added for enzymatic hydrolysis, and then 5% flavor protease is added for enzymatic hydrolysis after 5 minutes.

5. A whitening and anti-wrinkle protein active peptide composition according to claim 4, characterized in that: The enzymatic hydrolysis conditions are: enzymatic hydrolysis temperature 50°C, pH 6.4-7.7, and time 60 min.

6. The whitening and anti-wrinkle protein active peptide composition according to claim 1, characterized in that: The preparation method of Zingiber officinale active peptide is as follows: Step 1: washing, drying, crushing and detoxifying the leaves and stems of Zingiber officinale to obtain Zingiber officinale powder; Step 2: adding Zingiber officinale powder to deionized water to prepare a suspension with a concentration of 8%, then adding protease for enzymatic hydrolysis, performing enzyme inactivation treatment after enzymatic hydrolysis, filtering, and filtering the permeate through an ultrafiltration membrane with a molecular weight cutoff of 1200-2000Da to obtain Zingiber officinale active peptide.

7. The whitening and anti-wrinkle protein active peptide composition according to claim 6, characterized in that: In step 2, during enzymatic hydrolysis, alkaline protease is first added for enzymatic hydrolysis, and then flavor protease is added for enzymatic hydrolysis.

8. The whitening and anti-wrinkle protein active peptide composition according to claim 7, characterized in that: The enzymatic hydrolysis conditions of alkaline protease were as follows: 2% enzyme addition, 50°C enzymatic hydrolysis temperature, pH 7.0, and 75 min; the enzymatic hydrolysis conditions of flavor protease were as follows: 4% enzyme addition, 53°C enzymatic hydrolysis temperature, pH 6.4, and 50 min.

9. The method for preparing a whitening and anti-wrinkle protein active peptide composition according to any one of claims 1 to 8, characterized in that: The steps are: weighing the freeze-dried powder of each component according to weight, mixing them evenly, and obtaining a protein active peptide composition.

10. Use of the whitening and anti-wrinkle protein active peptide composition according to any one of claims 1 to 8 in the preparation of whitening and anti-wrinkle products.

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