Curcumin-loaded pectin sodium alginate gel as well as preparation method and application thereof

Abstract

The invention relates to curcumin-loaded pectin sodium alginate gel as well as a preparation method and application thereof, and belongs to the technical field of gel preparation. The preparation method of the curcumin-loaded pectin sodium alginate gel comprises the following steps: in a dark environment, stirring and mixing curcumin, arjunic acid, ortho-aminophenol and an organic solvent, adding a polyvinyl alcohol aqueous solution, a sodium alginate solution and a pectin solution, uniformly mixing, performing ultrasonic treatment, adding calcium chloride, stirring and mixing, performing ultrasonic treatment, and performing vacuum drying to obtain the curcumin-loaded pectin sodium alginate gel. And adding oxidized hyaluronic acid, continuously stirring, stirring at normal temperature until the organic solvent is completely volatilized, and cleaning the hydrogel with deionized water to obtain the hydrogel. According to the invention, calcium ions and sodium alginate are subjected to physical crosslinking, pectin and polyvinyl alcohol are combined to form a composite hydrogel layer, the compounding of multiple components can effectively control the dissolution and drug release behaviors of active components, the bioavailability of curcumin is increased, the compactness and stability of hydrogel are increased, and the bioavailability of curcumin is improved. The oxidation resistance of the hydrogel can be effectively improved.

Description

Technical Field

[0001] The invention belongs to the technical field of gel preparation and relates to a curcumin-loaded pectin sodium alginate gel, a preparation method and an application thereof. Background Art

[0002] Curcumin (CCM), a hydrophobic polyphenolic compound extracted from turmeric, is commonly used in the food industry as a spice and dietary supplement. Advances in research have revealed a variety of physiologically active functions of curcumin, including antioxidant, anti-inflammatory, antibacterial, antiviral, and antidiabetic properties. However, due to its low water solubility, poor chemical stability, poor gastrointestinal absorption, and rapid metabolism in the gastrointestinal tract and liver, direct application of curcumin in a variety of commercial foods, supplements, and pharmaceuticals is difficult. Therefore, the development of an efficient drug delivery system (DDS) is crucial. Existing research indicates that nanotechnology can address the limitations of curcumin's application, such as its conversion into liposomes, nanogels, nanoemulsions, polymer micelles, and various polymer nanoparticles. Curcumin-derived nanoparticles, with their high specific surface area and apparent solubility, can help overcome these issues, thereby improving its bioavailability and therapeutic efficacy. Summary of the Invention

[0003] The present invention aims to provide a curcumin-loaded pectin sodium alginate gel, a preparation method, and an application thereof. The present invention forms a composite hydrogel layer by physically cross-linking calcium ions with sodium alginate and combining pectin and polyvinyl alcohol. The composite hydrogel layer can effectively encapsulate o-aminophenol, arjunic acid, and curcumin, effectively controlling the dissolution and drug release of the active ingredients and increasing the bioavailability of curcumin. At the same time, the addition of oxidized hyaluronic acid can increase the density and stability of the hydrogel, thereby effectively improving the antioxidant capacity of the hydrogel.

[0004] The purpose of the present invention can be achieved through the following technical solutions:

[0005] A method for preparing a curcumin-loaded pectin sodium alginate gel comprises the following steps:

[0006] S1. In a light-proof environment, stirring and mixing curcumin, arjunic acid, o-aminophenol and an organic solvent to obtain a mixed solution;

[0007] S2. The mixed solution, polyvinyl alcohol aqueous solution, sodium alginate solution and pectin solution are mixed and then ultrasonically treated. Calcium chloride is added and stirred, and then oxidized hyaluronic acid is added and stirred continuously. The mixture is stirred at room temperature until the organic solvent is completely evaporated. The hydrogel is washed with deionized water to obtain the product.

[0008] As a preferred technical solution of the present invention, in step S1, the stirring and mixing is stirring at a rotation speed of 500-600 rpm for 30-40 minutes.

[0009] As a preferred technical solution of the present invention, in step S1, the mass ratio of curcumin, arjunic acid, o-aminophenol and organic solvent is 2.2-2.5:4.3-4.8:0.8-1.0:35-40.

[0010] As a preferred technical solution of the present invention, in step S1, the organic solvent is prepared by mixing ethyl acetate and methanol in a mass ratio of 9:0.8-1.0.

[0011] As a preferred technical solution of the present invention, in step S2, the ultrasonic treatment is performed at a power of 300-400 W for 4-5 minutes.

[0012] As a preferred technical solution of the present invention, in step S2, the stirring and mixing is carried out at a rotation speed of 400-500 rpm for 40-50 minutes; and the continued stirring is carried out at a temperature of 40-45° C. for 4-5 hours.

[0013] As a preferred technical solution of the present invention, in step S2, the mass ratio of the mixed solution, polyvinyl alcohol aqueous solution, sodium alginate solution, pectin solution, calcium chloride and oxidized hyaluronic acid is 15-18:45-50:22-25:16-18:2.0-2.2:0.25-0.28.

[0014] As a preferred technical solution of the present invention, in step S2, the mass fraction of the polyvinyl alcohol aqueous solution is 1.6-2.0%; the mass fraction of the sodium alginate solution is 2.5%; and the mass fraction of the pectin solution is 2%.

[0015] The invention discloses a curcumin-loaded pectin sodium alginate gel prepared by the above preparation method.

[0016] The invention discloses application of the curcumin-loaded pectin sodium alginate gel in the fields of cosmetics and medicine.

[0017] Beneficial effects of the present invention:

[0018] The present invention forms a composite hydrogel layer by physically cross-linking calcium ions with sodium alginate and combining pectin and polyvinyl alcohol. This effectively encapsulates o-aminophenol, arjunic acid, and curcumin, effectively controlling the dissolution and drug release of the active ingredients and increasing the bioavailability of curcumin. Meanwhile, the addition of oxidized hyaluronic acid increases the density and stability of the hydrogel, effectively improving the antioxidant capacity of the hydrogel. DETAILED DESCRIPTION

[0019] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0020] Example 1

[0021] A method for preparing a curcumin-loaded pectin sodium alginate gel comprises the following steps:

[0022] S1. In a light-proof environment, curcumin, arjunic acid, o-aminophenol, and an organic solvent are stirred and mixed to obtain a mixed solution; wherein the stirring and mixing is stirring at a speed of 500 rpm for 30 minutes; the mass ratio of the curcumin, arjunic acid, o-aminophenol, and organic solvent is 2.2:4.3:0.8:35; and the organic solvent is a mixture of ethyl acetate and methanol in a mass ratio of 9:0.8;

[0023] S2. Mix the mixed liquid, polyvinyl alcohol aqueous solution, sodium alginate solution and pectin solution and then perform ultrasonic treatment. Add calcium chloride and stir to mix, then add oxidized hyaluronic acid and continue stirring. Stir at room temperature until the organic solvent is completely volatilized, and wash the hydrogel with deionized water to obtain; wherein, the ultrasonic treatment is ultrasonication at a power of 300W for 4 minutes; the stirring and mixing is at a speed of 400rpm for 40 minutes; the continued stirring is stirring at a temperature of 40°C for 4 hours; the mass ratio of the mixed liquid, polyvinyl alcohol aqueous solution, sodium alginate solution, pectin solution, calcium chloride and oxidized hyaluronic acid is 15:45:22:16:2.0:0.25; the mass fraction of the polyvinyl alcohol aqueous solution is 1.6%; the mass fraction of the sodium alginate solution is 2.5%; the mass fraction of the pectin solution is 2%.

[0024] Example 2

[0025] A method for preparing a curcumin-loaded pectin sodium alginate gel comprises the following steps:

[0026] S1. In a light-proof environment, curcumin, arjunic acid, o-aminophenol, and an organic solvent are stirred and mixed to obtain a mixed solution; wherein the stirring and mixing is stirring at a speed of 550 rpm for 35 minutes; the mass ratio of the curcumin, arjunic acid, o-aminophenol, and organic solvent is 2.4:4.6:0.9:38; and the organic solvent is a mixture of ethyl acetate and methanol in a mass ratio of 9:0.9;

[0027] S2. Mix the mixed liquid, polyvinyl alcohol aqueous solution, sodium alginate solution and pectin solution, then perform ultrasonic treatment, add calcium chloride and stir to mix, add oxidized hyaluronic acid and continue stirring, stir at room temperature until the organic solvent is completely volatilized, and wash the hydrogel with deionized water to obtain; wherein, the ultrasonic treatment is ultrasonication at a power of 350W for 4.5 minutes; the stirring and mixing is at a speed of 450rpm for 45 minutes; the continued stirring is stirring at a temperature of 42°C for 4.5 hours; the mass ratio of the mixed liquid, polyvinyl alcohol aqueous solution, sodium alginate solution, pectin solution, calcium chloride and oxidized hyaluronic acid is 16:48:24:17:2.1:0.26; the mass fraction of the polyvinyl alcohol aqueous solution is 1.8%; the mass fraction of the sodium alginate solution is 2.5%; the mass fraction of the pectin solution is 2%.

[0028] Example 3

[0029] A method for preparing a curcumin-loaded pectin sodium alginate gel comprises the following steps:

[0030] S1. In a light-proof environment, curcumin, arjunic acid, o-aminophenol, and an organic solvent are stirred and mixed to obtain a mixed solution; wherein the stirring and mixing is stirring at a speed of 600 rpm for 40 minutes; the mass ratio of the curcumin, arjunic acid, o-aminophenol, and organic solvent is 2.5:4.8:1.0:40; and the organic solvent is a mixture of ethyl acetate and methanol in a mass ratio of 9:1.0;

[0031] S2. Mix the mixed liquid, polyvinyl alcohol aqueous solution, sodium alginate solution and pectin solution and then perform ultrasonic treatment. Add calcium chloride and stir to mix, then add oxidized hyaluronic acid and continue stirring. Stir at room temperature until the organic solvent is completely volatilized, and wash the hydrogel with deionized water to obtain; wherein, the ultrasonic treatment is ultrasonication at a power of 400W for 5 minutes; the stirring and mixing is at a speed of 500rpm for 50 minutes; the continued stirring is stirring at a temperature of 45°C for 5 hours; the mass ratio of the mixed liquid, polyvinyl alcohol aqueous solution, sodium alginate solution, pectin solution, calcium chloride and oxidized hyaluronic acid is 18:50:25:18:2.2:0.28; the mass fraction of the polyvinyl alcohol aqueous solution is 2.0%; the mass fraction of the sodium alginate solution is 2.5%; the mass fraction of the pectin solution is 2%.

[0032] Comparative Examples 1-3

[0033] Compared with Example 3, the difference between Comparative Examples 1-3 is that the amounts of curcumin, arjunic acid, and o-aminophenol are as shown in Table 1, and the other components, preparation steps, and parameters are the same.

[0034] Table 1 (Unit: parts by weight)

[0035] Curcumin Arjunic acid o-Aminophenol Example 3 2.5 4.8 1.0 Comparative Example 1 0 6.8 2.5 Comparative Example 2 4.9 0 3.4 Comparative Example 3 3.0 5.3 0

[0036] Comparative Example 4

[0037] Compared with Example 3, Comparative Example 4 is different in that calcium chloride is not used, and the remaining components, preparation steps and parameters are the same.

[0038] Comparative Example 5

[0039] Compared with Example 3, Comparative Example 5 is different in that oxidized hyaluronic acid is not used, and the other components, preparation steps and parameters are the same.

[0040] The hydrogels prepared in Examples 1-3 and Comparative Examples 1-5 were subjected to the following performance tests, and the test results are shown in Table 2.

[0041] Antioxidant performance test:

[0042] The ABTS working solution was diluted with PBS (0.1 M, pH 7.4) so that its absorbance at 734 nm reached 0.70 ± 0.02. Subsequently, the sample was thoroughly mixed with the ABTS solution. After incubation at 25°C for 10 minutes, the absorbance at 734 nm was measured using a microplate reader. The scavenging activity of the hydrogel was determined using the following formula. Each sample was measured three times, and the results were expressed as the mean ± standard deviation of the three measurements for each sample.

[0043] ABTS clearance (%) = (1-A1 / A0) × 100%;

[0044] DPPH was prepared with anhydrous ethanol to prepare 2×10 -4 mol / L solution, prepare several 2mL portions of the test solution, anhydrous ethanol, and DPPH solution using the hydrogel sample. Mix them well and place them at room temperature for 30 minutes. Measure the absorbance at a wavelength of 517nm to obtain A2. Measure each sample in parallel three times and take the average value. Use Vc as the positive control, and measure the absorbance value using anhydrous ethanol instead of DPPH as the control A1 blank. Measure the absorbance A0 of a mixture of DPPH solution and anhydrous ethanol. Calculate the scavenging rate of each test sample for DPPH free radicals using the following formula;

[0045] DPPH clearance rate (%) = (1-(A2-A1) / A0) × 100%;

[0046] Table 2

[0047] ABTS clearance rate (%) DPPH clearance rate (%) Example 1 95.6 99.1 Example 2 95.2 98.6 Example 3 95.9 99.3 Comparative Example 1 72.7 75.4 Comparative Example 2 79.2 82.5 Comparative Example 3 82.3 86.7 Comparative Example 4 71.8 75.3 Comparative Example 5 80.5 84.8

[0048] From the test results in Table 2, it can be seen that compared with Comparative Examples 1-5, the hydrogels prepared in Examples 1-3 have excellent antioxidant capacity.

[0049] The hydrogels prepared in Examples 1-3 and Comparative Examples 1-5 were placed at room temperature for 15 days and then tested for their antioxidant properties according to the above method to evaluate their storage stability. The results are shown in Table 3.

[0050] Table 3

[0051] ABTS clearance rate (%) DPPH clearance rate (%) Example 1 93.4 97.0 Example 2 93.3 96.5 Example 3 93.7 97.2 Comparative Example 1 67.7 70.4 Comparative Example 2 73.2 76.3 Comparative Example 3 78.2 82.4 Comparative Example 4 73.4 67.8 Comparative Example 5 74.9 79.5

[0052] From the test results in Table 3, it can be seen that compared with Comparative Examples 1-5, the hydrogels prepared in Examples 1-3 have excellent storage stability.

[0053] Through comparative analysis of Table 1 and Table 2 in combination with Examples 1-3 and Comparative Examples 1-5, it can be seen that this is because the present invention forms a composite hydrogel layer by physically cross-linking calcium ions with sodium alginate and combining pectin and polyvinyl alcohol, which can effectively encapsulate o-aminophenol, arjunalic acid and curcumin, effectively control the dissolution and drug release behavior of the active ingredients, and increase the bioavailability of curcumin; arjunalic acid belongs to a type of pentacyclic triterpenoid compound and has good free radical scavenging activity. It contains a hydrophobic cyclic skeleton and hydrophilic side chains, and can form self-assembled supramolecules in a variety of solvents. Its highly rigid skeleton can help maintain its nanostructure to encapsulate curcumin, resulting in enhanced protective effect and further increasing the stability and free radical scavenging ability of curcumin; o-aminophenol contains amino groups that can form an interactive force with curcumin and arjunalic acid, while increasing its own stability, and can also form a chemical bond with oxidized hyaluronic acid. Combined with its own benzene ring structure, it can increase the density and stability of the hydrogel, and can effectively improve the antioxidant capacity of the hydrogel.

[0054] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as a preferred embodiment as above, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments using the technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any indirect modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A method for preparing a curcumin-loaded pectin sodium alginate gel, characterized in that: The preparation method comprises the following steps: S1. In a light-proof environment, stirring and mixing curcumin, arjunic acid, o-aminophenol and an organic solvent to obtain a mixed solution; S2. The mixed solution, polyvinyl alcohol aqueous solution, sodium alginate solution and pectin solution are mixed and then ultrasonically treated. Calcium chloride is added and stirred, and then oxidized hyaluronic acid is added and stirred continuously. The mixture is stirred at room temperature until the organic solvent is completely evaporated. The hydrogel is washed with deionized water to obtain the product.

2. The method for preparing a curcumin-loaded pectin sodium alginate gel according to claim 1, wherein: In step S1, the stirring and mixing is carried out at a rotation speed of 500-600 rpm for 30-40 minutes.

3. The method for preparing a curcumin-loaded pectin sodium alginate gel according to claim 1, wherein: In step S1, the mass ratio of curcumin, arjunic acid, o-aminophenol and organic solvent is 2.2-2.5:4.3-4.8:0.8-1.0:35-40.

4. The method for preparing a curcumin-loaded pectin sodium alginate gel according to claim 1, wherein: In step S1, the organic solvent is prepared by mixing ethyl acetate and methanol in a mass ratio of 9:0.8-1.

0.

5. The method for preparing a curcumin-loaded pectin sodium alginate gel according to claim 1, wherein: In step S2, the ultrasonic treatment is performed at a power of 300-400 W for 4-5 minutes.

6. The method for preparing a curcumin-loaded pectin sodium alginate gel according to claim 1, wherein: In step S2, the stirring and mixing is carried out at a rotation speed of 400-500 rpm for 40-50 minutes; and the continued stirring is carried out at a temperature of 40-45° C. for 4-5 hours.

7. The method for preparing a curcumin-loaded pectin sodium alginate gel according to claim 1, wherein: In step S2, the mass ratio of the mixed solution, polyvinyl alcohol aqueous solution, sodium alginate solution, pectin solution, calcium chloride and oxidized hyaluronic acid is 15-18:45-50:22-25:16-18:2.0-2.2:0.25-0.

28.

8. The method for preparing a curcumin-loaded pectin sodium alginate gel according to claim 1, wherein: In step S2, the mass fraction of the polyvinyl alcohol aqueous solution is 1.6-2.0%; the mass fraction of the sodium alginate solution is 2.5%; and the mass fraction of the pectin solution is 2%.

9. A curcumin-loaded pectin sodium alginate gel prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the curcumin-loaded pectin sodium alginate gel according to claim 9 in the fields of cosmetics and medicine.