Polypeptide concentration device and concentration method thereof

Abstract

The invention belongs to the technical field of concentration equipment, and particularly relates to a polypeptide concentration device and a concentration method.The polypeptide concentration device comprises a hollow concentration box, a supporting frame is fixedly connected to the bottom end of the hollow concentration box, two partition plates are fixedly connected to the inner wall of the hollow concentration box in an inclined mode, and mounting through holes are formed in the outer walls of the two partition plates; first sealing bearings are fixedly connected to the hole walls of the mounting through holes, and the inner walls of the inner rings of the two first sealing bearings are jointly and fixedly connected with a concentration mechanism. The polypeptide concentration device not only has the capability of efficiently concentrating polypeptide, but also has the functions of self-cleaning the concentration channel, controlling the concentration temperature and automatically defoaming the polypeptide liquid, so that the quality of the concentrated polypeptide is improved, and meanwhile, the situation that the polypeptide drills into the concentration channel to cause waste can be avoided; not only can the use reliability and convenience of the polypeptide concentration device be improved, but also the labor intensity of workers is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of concentration equipment, and in particular relates to a polypeptide concentration device and a concentration method thereof. Background Art

[0002] A polypeptide is a compound composed of 2 to 100 amino acids linked by peptide bonds. Its structure is between that of amino acids and proteins, and it combines the activity of small molecules with the functional properties of large molecules. It plays important roles in hormone regulation and immune defense in organisms, such as insulin and antimicrobial peptides. Due to its high specificity and low toxicity, polypeptides are widely used in pharmaceutical research and development, functional foods, and cosmetics. Concentrated polypeptides can significantly improve their stability and reduce the risk of degradation due to low concentrations. At the same time, it can reduce the volume of the solution and facilitate storage and transportation. In addition, high-concentration polypeptides are a necessary condition for downstream experiments and production such as drug preparation and activity determination, and can effectively improve process efficiency and product quality. For example, the patent with authorization announcement number CN211367448U discloses a concentration device for polypeptides.

[0003] Currently, the concentration process of peptide liquid through the device mostly uses centrifugal filtration, but this concentration method has the following problems:

[0004] First, during the concentration process, some peptides tend to clog the concentration channel, slowing down the concentration rate of the peptide liquid. As the peptide concentration time increases, the number of blocked peptide concentration channels in the device increases, seriously affecting the efficiency of peptide concentration. In order to maintain the operation of the equipment, the concentration channel needs to be cleaned frequently, which not only significantly increases labor maintenance costs, but also reduces the long-term operation stability and operational convenience of the device.

[0005] Second, heat-sensitive polypeptides need to be frozen first and then heated to evaporate the water for concentration. This concentration method is not only cumbersome and inefficient, but the evaporation concentration process also easily exposes some polypeptides. In addition, the evaporation concentration process is in a high-temperature environment, and the polypeptides are modified and inactivated, which affects the quality of the polypeptide concentration and the reliability of the polypeptide concentration device.

[0006] Therefore, we propose a polypeptide concentration device and a polypeptide concentration method to solve the above problems. Summary of the Invention

[0007] The object of the present invention is to provide a polypeptide concentration device and a concentration method thereof in order to solve the above problems.

[0008] To achieve the above objectives, the present invention adopts the following technical solutions: a polypeptide concentrating device comprising a hollow concentrating tank, wherein the bottom end of the hollow concentrating tank is fixedly connected to a support frame, and the inner wall of the hollow concentrating tank is fixedly connected to two partitions at an angle, wherein the outer walls of the two partitions are each provided with a mounting through-hole, and the hole walls of the mounting through-holes are fixedly connected to a first sealed bearing, and the inner walls of the inner rings of the two first sealed bearings are fixedly connected to a concentrating mechanism;

[0009] A cleaning mechanism is fixedly connected to the upper surface of the hollow concentrating tank;

[0010] The outer wall of the bottom end of the support frame is fixedly connected with a PLC controller and a concentration acceleration mechanism;

[0011] A through hole is formed on the upper surface of the hollow concentrating tank, and a guide hopper is fixedly connected to the wall of the through hole, and the bottom end of the guide hopper is located inside the feed end of the concentrating mechanism;

[0012] A touch screen control panel is fixedly connected to the outer wall of the hollow concentration box.

[0013] In the above-mentioned polypeptide concentrating device, the concentrating mechanism includes a ceramic filter cartridge fixedly connected to the inner walls of the inner rings of two first sealed bearings, the inner wall of the ceramic filter cartridge is fixedly connected to a water-permeable concentrating membrane sleeve, the bottom end of the ceramic filter cartridge is fixedly sleeved with an outer gear ring, the side wall of the hollow concentrating box is provided with a fixing hole, and the hole wall of the fixing hole is fixedly connected to a discharge hopper, the outer wall of one of the partitions is fixedly connected to a driving motor, the outer wall of the partition is provided with a through hole, and the hole wall of the through hole is fixedly connected to a second sealed bearing, the driving end of the driving motor passes through the second sealed bearing, the outer wall of the driving motor is in sealing contact with the inner wall of the inner ring of the second sealed bearing, the driving end of the driving motor is fixedly connected to a gear meshing with the outer gear ring, the inner wall of the hollow concentrating box is fixedly connected to two horizontal plates, and the opposite sides of the two horizontal plates are commonly fixedly connected to a sealing strip, and the arcuate surface of the sealing strip is in sealing contact with the arcuate outer wall of the ceramic filter cartridge.

[0014] In the above-mentioned polypeptide concentration device, the two partitions divide the inner cavity of the hollow concentration box into a feed area, a processing area and a discharge area, and the two transverse plates divide the processing area into an air intake area and a negative pressure area.

[0015] In the above-mentioned polypeptide concentration device, the cleaning mechanism includes an insulation box fixedly connected to the upper surface of the hollow concentration box, the inner wall of the insulation box is fixedly connected with an insulation board, the insulation board and the outer wall of the insulation box are both provided with circular holes, and the hole walls of the two circular holes are commonly fixedly connected with a metal coil, the upper surface of the insulation box is provided with a through hole, and the hole wall of the through hole is fixedly connected with a metal heat conducting plate, the lower surface of the metal heat conducting plate is in contact with the upper surface of the metal coil, the upper surface of the metal heat conducting plate is fixedly connected with a plurality of semiconductor refrigeration plates, the heat dissipation sides of the plurality of semiconductor refrigeration plates are fixedly connected with a heat dissipation mesh block, the upper surfaces of the plurality of heat dissipation mesh blocks are commonly fixedly connected with a heat dissipation fan, the side of the insulation box away from the metal coil is fixedly connected with an air outlet pipe, the bottom end of the air outlet pipe passes through the top of the hollow concentration box and is connected with the cavity of the air inlet area, and the pipe wall of the air outlet pipe is fixedly connected with a temperature sensor.

[0016] In the above-mentioned polypeptide concentration device, the concentration acceleration mechanism includes a collecting box fixedly connected to the outer wall of the bottom end of the support frame, the upper surface of the collecting box is fixedly connected with a liquid inlet pipe and a curved pipe, the top end of the liquid inlet pipe is fixedly connected to the bottom end of the hollow concentration box, the top end of the liquid inlet pipe is connected to the cavity in the negative pressure zone, the upper surface of the collecting box is fixedly connected with an air pump, the air inlet end of the air pump is fixedly connected to the top end of the curved pipe, the air outlet end of the air pump is fixedly connected with a return pipe, the air outlet end of the return pipe is fixedly connected to the air inlet end of the metal coil, the upper surface of the collecting box is provided with a mounting hole, and the hole wall of the mounting hole is fixedly connected with a liquid level sensor.

[0017] In the above-mentioned polypeptide concentration device, the bottom end of the side wall of the collection box is fixedly connected to an electromagnetic drain valve, and the water outlet end of the electromagnetic drain valve is fixedly connected to a drainage pipe.

[0018] In the above-mentioned polypeptide concentration device, the bottom end of the side wall of the heat-insulating box is fixedly connected to a drainage branch pipe, and the bottom end of the drainage branch pipe is fixedly connected to the pipe wall of the drainage pipe.

[0019] A method for concentrating a polypeptide using a concentrating device, the method comprising the following steps:

[0020] Step S1: First, a start signal is sent to the PLC controller through the touch screen control panel. The PLC controller controls the drive motor and the air pump to start according to the received start signal. The drive motor causes the ceramic filter cartridge to rotate slowly. The air pump puts the negative pressure area into a negative pressure environment. The air sucked by the air pump enters the air intake area through the return pipe and the cleaning mechanism.

[0021] Step S2: The polypeptide liquid is then poured into the concentration mechanism through the guide hopper. The polypeptide liquid is then subjected to the negative pressure suction of the negative pressure zone and quickly passes through the tiny holes of the permeable concentration membrane sleeve and the ceramic filter cartridge into the negative pressure zone of the hollow concentration tank, achieving the purpose of rapid concentration.

[0022] Step S3: In step S2, the driving motor drives the ceramic filter cartridge to rotate slowly, and the cleaning mechanism cooperates, so that the polypeptide liquid will never block the concentration channel during the process of being discharged to the outlet at the bottom end of the ceramic filter cartridge;

[0023] Step S4: Before step S2, the staff presets a warning temperature T0 based on the temperature adaptability of the polypeptide itself, and the PLC controller also controls the cooling fan and multiple semiconductor refrigeration chips to energize and work. The multiple semiconductor refrigeration chips cool the air entering the metal coil through the metal heat conducting plate. While cooling the air, they can also liquefy the moisture carried by the air. The cooled air enters the outlet pipe and is detected by the temperature sensor. Then, the cleaning mechanism adjusts the power of the cooling fan according to the temperature value detected by the temperature sensor to adjust the temperature of the air entering the air intake area.

[0024] Step S5: When the polypeptide concentration device is working, the ceramic filter cartridge rotates slowly, the polypeptide liquid concentration process does not move violently, and the amount of foam generated on the surface of the polypeptide liquid is small. At the same time, after the air in the air inlet area passes through the concentration channel and enters the inside of the water-permeable concentration membrane sleeve, the air flow can blow away a small amount of foam on the surface of the polypeptide liquid. In addition, the negative pressure suction force of the negative pressure area will further suck the air in the foam on the surface of the polypeptide liquid, thereby achieving the purpose of defoaming.

[0025] Compared with existing technologies, the advantages of a polypeptide concentration device and a polypeptide concentration method are:

[0026] 1. Through the provided concentration mechanism, hollow concentration box and vacuum pump, when the polypeptide needs to be concentrated, the driving motor and the vacuum pump are first controlled by the touch screen control panel and the PLC controller to start, the driving motor causes the ceramic filter cartridge to rotate slowly, the vacuum pump puts the negative pressure area in a negative pressure environment, and the air sucked by the vacuum pump enters the air intake area through the return pipe and the cleaning mechanism, and then the polypeptide liquid is poured into the concentration mechanism through the guide hopper. Under the action of the negative pressure suction force of the negative pressure area, the polypeptide solution will quickly pass through the water-permeable concentration membrane sleeve and the tiny holes of the ceramic filter cartridge into the negative pressure area of the hollow concentration box, and due to the slow rotation of the ceramic filter cartridge and the cooperation of the cleaning mechanism, the polypeptide liquid concentration process can be ensured to be efficient and smooth. This mechanism enables the polypeptide concentration device not only to have the ability to efficiently concentrate polypeptides, but also to have the function of self-cleaning of the concentration channel, which not only improves the reliability and convenience of the use of the polypeptide concentration device, but also reduces the labor intensity of the staff.

[0027] 2. Through the cleaning mechanism, before the polypeptide concentration device is started, the staff presets the warning temperature T0 according to the temperature adaptability characteristics of the polypeptide itself, and the PLC controller also controls the cooling fan and multiple semiconductor refrigeration chips to energize and work. The multiple semiconductor refrigeration chips cool the air entering the metal coil through the metal heat conduction plate. While cooling the air, it can also liquefy the moisture carried by the air. The cooled air enters the outlet pipe and is detected by the temperature sensor. The cleaning mechanism then adjusts the power of the cooling fan according to the temperature value detected by the temperature sensor. The increase in the power of the cooling fan can blow more air to the heat dissipation mesh block and the heat dissipation side of the semiconductor refrigeration chip, thereby accelerating the heat dissipation of the heat dissipation side of the semiconductor refrigeration chip and the heat transfer speed of the semiconductor refrigeration chip, thereby improving the cooling effect of the cooling side of the semiconductor refrigeration chip, so that the temperature of the air entering the air inlet area is lower than the warning temperature T0, thereby avoiding the interference of excessively high temperature air on the activity of the polypeptide and ensuring the quality of the concentrated polypeptide. This mechanism enables the polypeptide concentration device to have the function of concentration temperature control, improves the protection of the concentration process of heat-sensitive polypeptides, and thus improves the quality of the concentrated heat-sensitive polypeptides.

[0028] 3. Through the ceramic filter cartridge and concentration mechanism, when the polypeptide concentration device is working, the ceramic filter cartridge rotates slowly, the polypeptide liquid concentration process does not move violently, and the amount of foam generated on the surface of the polypeptide liquid is small. At the same time, after the air in the air inlet area passes through the concentration channel and enters the inside of the water-permeable concentration membrane sleeve, the airflow can blow away a small amount of foam on the surface of the polypeptide liquid. In addition, the negative pressure suction force in the negative pressure area will further suck the air in the foam on the surface of the polypeptide liquid and achieve the purpose of defoaming. The defoaming process does not require the addition of a defoaming agent. In addition, the ceramic filter cartridge can not only drive the water-permeable concentration membrane sleeve to rotate and clean itself, but also support and protect the water-permeable concentration membrane sleeve to avoid contamination and damage on the back of the water-permeable concentration membrane sleeve. This mechanism enables the polypeptide concentration device to have the function of automatic defoaming of the polypeptide liquid, thereby improving the reliability of the use of the polypeptide concentration device. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic structural diagram of a polypeptide concentration device and a concentration method thereof provided by the present invention;

[0030] Figure 2 yes Figure 1 Schematic diagram of the structure in cross-section;

[0031] Figure 3 yes Figure 2 Schematic diagram of the structure of the cross section of the ceramic filter cartridge;

[0032] Figure 4 yes Figure 2 A schematic diagram of the enlarged structure of the intermediate concentration mechanism;

[0033] Figure 5 yes Figure 2Schematic diagram of the three-dimensional structure of the middle insulation box;

[0034] Figure 6 yes Figure 2 A schematic diagram of the three-dimensional structure of the collection box;

[0035] Figure 7 yes Figure 2 Schematic diagram of the structure of the cleaning mechanism;

[0036] Figure 8 yes Figure 2 Schematic diagram of the structure of the intermediate concentration acceleration mechanism.

[0037] In the figure: 1 hollow concentration box, 2 support frame, 3 partition, 4 first sealing bearing, 5 concentration mechanism, 51 ceramic filter cartridge, 52 permeable concentration membrane sleeve, 53 external gear ring, 54 discharge hopper, 55 drive motor, 56 second sealing bearing, 57 gear, 58 cross plate, 59 sealing strip, 6 cleaning mechanism, 61 heat insulation box, 62 heat insulation board, 63 metal coil, 64 metal heat conducting plate, 65 semiconductor refrigeration plate, 66 heat dissipation mesh block, 67 heat dissipation fan, 68 outlet pipe, 69 temperature sensor, 7 PLC controller, 8 concentration acceleration mechanism, 81 collection box, 82 liquid inlet pipe, 83 elbow, 84 vacuum pump, 85 return pipe, 86 liquid level sensor, 9 guide hopper, 10 touch screen control panel, 11 feed area, 12 discharge area, 13 air inlet area, 14 negative pressure area, 15 electromagnetic drain valve, 16 drain pipe, 17 drain branch pipe. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0039] like Figures 1-8As shown, a polypeptide concentration device comprises a hollow concentration box 1, the bottom end of the hollow concentration box 1 is fixedly connected to a support frame 2, the inner wall of the hollow concentration box 1 is obliquely fixedly connected to two partitions 3, the outer walls of the two partitions 3 are provided with mounting through holes, and the hole walls of the mounting through holes are fixedly connected to first sealed bearings 4, the inner walls of the inner rings of the two first sealed bearings 4 are jointly fixedly connected to a concentrating mechanism 5, the concentrating mechanism 5 comprises a ceramic filter cartridge 51 fixedly connected to the inner walls of the inner rings of the two first sealed bearings 4, the inner wall of the ceramic filter cartridge 51 is fixedly connected to a water-permeable concentrating membrane sleeve 52, the bottom end of the ceramic filter cartridge 51 is fixedly sleeved with an outer gear ring 53, the side wall of the hollow concentration box 1 is provided with a fixing hole, and the hole wall of the fixing hole is fixedly connected to a discharge hopper 54, the outer wall of one of the partitions 3 is fixed It is connected to a drive motor 55, and a through hole is opened on the outer wall of the partition 3, and a second sealed bearing 56 is fixedly connected to the wall of the through hole. The driving end of the drive motor 55 passes through the second sealed bearing 56, and the outer wall of the drive motor 55 is in sealing contact with the inner wall of the inner ring of the second sealed bearing 56. The driving end of the drive motor 55 is fixedly connected to a gear 57 meshing with the outer gear ring 53. The inner wall of the hollow concentrating tank 1 is fixedly connected to two horizontal plates 58, and the opposite sides of the two horizontal plates 58 are fixedly connected with a sealing strip 59. The arcuate surface of the sealing strip 59 is in sealing contact with the arcuate outer wall of the ceramic filter cartridge 51. The two partitions 3 divide the internal cavity of the hollow concentrating tank 1 into a feeding area 11, a processing area and a discharging area 12. The two horizontal plates 58 divide the processing area into an air intake area 13 and a negative pressure area 14.

[0040] The upper surface of the hollow concentration box 1 is fixedly connected to a cleaning mechanism 6, which includes a heat-insulating box 61 fixedly connected to the upper surface of the hollow concentration box 1, and a heat-insulating plate 62 fixedly connected to the inner wall of the heat-insulating box 61. The heat-insulating plate 62 and the outer wall of the heat-insulating box 61 are both provided with circular holes, and the hole walls of the two circular holes are fixedly connected to a metal coil 63. The upper surface of the heat-insulating box 61 is provided with a through hole, and the hole wall of the through hole is fixedly connected to a metal heat-conducting plate 64. The lower surface of the metal heat-conducting plate 64 contacts the upper surface of the metal coil 63, and the upper surface of the metal heat-conducting plate 64 is fixedly connected to multiple The semiconductor refrigeration plate 65, the heat dissipation side of multiple semiconductor refrigeration plates 65 are fixedly connected to a heat dissipation mesh block 66, the upper surfaces of multiple heat dissipation mesh blocks 66 are commonly fixedly connected to a heat dissipation fan 67, the side of the heat insulation box 61 away from the metal coil 63 is fixedly connected to an air outlet pipe 68, the bottom end of the air outlet pipe 68 passes through the top of the hollow concentration box 1 and is connected to the cavity of the air inlet area 13, and the wall of the air outlet pipe 68 is fixedly connected to a temperature sensor 69. This mechanism enables the polypeptide concentration device to have the function of self-cleaning the concentration channel and avoids the situation where the polypeptide drills into the concentration channel and causes waste.

[0041] The outer wall of the bottom end of the support frame 2 is fixedly connected to the PLC controller 7 and the concentration acceleration mechanism 8. The concentration acceleration mechanism 8 includes a collection box 81 fixedly connected to the outer wall of the bottom end of the support frame 2. The bottom end of the side wall of the collection box 81 is fixedly connected to the electromagnetic drain valve 15. The water outlet end of the electromagnetic drain valve 15 is fixedly connected to the drain pipe 16. The upper surface of the collection box 81 is fixedly connected to the liquid inlet pipe 82 and the elbow 83. The top end of the liquid inlet pipe 82 is fixedly connected to the bottom end of the hollow concentration box 1. The top end of the liquid inlet pipe 82 is connected to the cavity of the negative pressure area 14. The collection box 8 1 is fixedly connected to the upper surface of the air pump 84. The air inlet end of the air pump 84 is fixedly connected to the top end of the curved pipe 83. The air outlet end of the air pump 84 is fixedly connected to the return pipe 85. The air outlet end of the return pipe 85 is fixedly connected to the air inlet end of the metal coil 63. The upper surface of the collection box 81 is provided with a mounting hole, and the hole wall of the mounting hole is fixedly connected to a liquid level sensor 86. This mechanism enables the polypeptide concentrator to have the ability to efficiently concentrate polypeptides, which not only improves the reliability and convenience of the polypeptide concentrator, but also reduces the labor intensity of the staff.

[0042] A through hole is provided on the upper surface of the hollow concentrating tank 1, and a guide hopper 9 is fixedly connected to the wall of the through hole. The bottom end of the guide hopper 9 is located inside the feed end of the concentrating mechanism 5. The outer wall of the hollow concentrating tank 1 is fixedly connected to a touch screen control panel 10. The bottom end of the side wall of the insulation box 61 is fixedly connected to a drainage branch pipe 17. The bottom end of the drainage branch pipe 17 is fixedly connected to the wall of the drainage pipe 16. The drainage branch pipe 17 can discharge the water accumulated in the insulation box 61 in time.

[0043] The drive motor 55, semiconductor refrigeration plate 65, vacuum pump 84, cooling fan 67 and electromagnetic drain valve 15 are all electrically connected to the output end of the PLC controller 7 through wires, and the temperature sensor 69, liquid level sensor 86 and touch screen control panel 10 are all electrically connected to the input end of the PLC controller 7 through wires. The above-mentioned channel equipment and electrical connections are all existing technologies and will not be repeated here.

[0044] The operating principle of the present invention is described as follows: when the polypeptide liquid needs to be concentrated, a start signal is first sent to the PLC controller 7 through the touch screen control panel 10. The PLC controller 7 controls the drive motor 55 and the air pump 84 to start according to the received start signal. The drive motor 55 causes the ceramic filter cartridge 51 to rotate slowly through the gear 57 and the outer gear ring 53. The air pump 84 sucks the air in the collection box 81 through the bend 83. The collection box 81 sucks the air from the negative pressure area 14 through the liquid inlet pipe 82, so that the negative pressure area 14 is in a negative pressure environment. The negative pressure environment is replenished by the air sucked by the ceramic filter cartridge 51 and the water permeable concentration membrane sleeve 52 located inside the negative pressure area 14. The air passes through the concentration channel and enters the negative pressure area 14 to achieve the purpose of maintaining air pressure balance. The air sucked by the air pump 84 enters the air inlet area 13 through the return pipe 85 and the cleaning mechanism 6. Finally, the air passes through the ceramic filter cartridge 51 and the water permeable concentration membrane sleeve 52 located in the air inlet area 13 and finally enters the interior of the water permeable concentration membrane sleeve 52.

[0045] Then the polypeptide liquid is poured into the concentration mechanism 5 through the guide hopper 9. At this time, the polypeptide liquid solution is affected by the negative pressure suction of the negative pressure area 14 and will quickly pass through the tiny holes (i.e., the concentration channel) of the water-permeable concentration membrane sleeve 52 and the ceramic filter cartridge 51 to enter the negative pressure area 14 of the hollow concentration tank 1. Since the driving motor 55 drives the ceramic filter cartridge 51 to rotate slowly through the gear 57 and the outer gear ring 53, the polypeptide liquid will never encounter the situation of polypeptide blocking the concentration channel during the process of being discharged to the outlet at the bottom end of the ceramic filter cartridge 51. Specifically, the air sucked by the vacuum pump 84 will be injected into the metal coil 63 through the return pipe 85. The metal coil The air in 63 then enters the air inlet area 13 of the hollow concentration box 1 through the air outlet pipe 68, and the air in the air inlet area 13 passes through the ceramic filter cartridge 51 and the top of the water-permeable concentration membrane sleeve 52. The process of the air entering the water-permeable concentration membrane sleeve 52 will backwash and clean the concentration channel blocked by the polypeptide, so that the polypeptide liquid will not encounter the problem of the concentration channel being blocked during the discharge process to the bottom outlet of the ceramic filter cartridge 51, and at the same time, it can avoid the situation where the polypeptide drills into the concentration channel and is wasted. After concentration, the polypeptide is discharged from the bottom outlet of the ceramic filter cartridge 51 into the discharge hopper 54 along with a small amount of solution, and is discharged through the discharge hopper 54, thereby achieving the purpose of concentrating the polypeptide liquid;

[0046] During this process, the solution in the negative pressure area 14 enters the collection box 81 through the liquid inlet pipe 82 for temporary storage. Eventually, the solution in the collection box 81 will submerge the bottom end of the liquid inlet pipe 82. At the same time, the liquid level sensor 86 will monitor the liquid level of the solution in the collection box 81 in real time, and convert the liquid level data into an electrical signal and transmit it to the PLC controller 7. If the liquid level data monitored by the liquid level sensor 86 reaches the warning liquid level range preset by the PLC controller 7, the PLC controller 7 controls the electromagnetic drain valve 15 to be energized and opened, and the solution in the collection box 81 is discharged through the drain pipe 16. If the liquid level in the collection box 81 does not reach the warning range preset by the PLC controller 7, the PLC controller 7 does not control the electromagnetic drain valve 15 to open. This mechanism enables the polypeptide concentrator to not only have the ability to efficiently concentrate polypeptides, but also have the function of self-cleaning the concentration channel, and avoid the situation where polypeptides drill into the concentration channel and cause waste. It can not only improve the reliability and convenience of the polypeptide concentrator, but also reduce the labor intensity of the staff.

[0047] Before the polypeptide concentration device is started, the staff presets the warning temperature T0 according to the temperature adaptability of the polypeptide itself. Specifically, the staff sets the warning temperature T0 in the PLC controller 7 through the touch screen control panel 10. At the same time, the PLC controller 7 also controls the cooling fan 67 and multiple semiconductor refrigeration plates 65 to be powered on. The multiple semiconductor refrigeration plates 65 cool the air entering the metal coil 63 through the metal heat conducting plate 64. While cooling the air, they can also liquefy the moisture carried by the air. After the moisture is liquefied into water droplets, it is carried by the air in the metal coil 63 and discharged into the heat insulation box 61. Finally, the water liquid gathered by the water droplets is discharged into the drain pipe 16 through the drain branch pipe 17. The cooled air enters the outlet pipe 68 and is detected by the temperature sensor 69. The temperature sensor 69 converts the detected temperature value into an electrical signal and transmits it to the PLC controller 7. If the temperature is lower than the warning temperature T0 preset by the PLC controller 7, the PLC controller 7 can reduce the power of the cooling fan 67. The power consumption of the cooling fan 67 is reduced to achieve the purpose of energy saving. If the temperature detected by the temperature sensor 69 exceeds the warning temperature T0 preset by the PLC controller 7, the PLC controller 7 immediately increases the power of the cooling fan 67. The increased power of the cooling fan 67 can blow more air to the heat dissipation mesh block 66 and the heat dissipation side of the semiconductor refrigeration plate 65, thereby accelerating the heat dissipation of the heat dissipation side of the semiconductor refrigeration plate 65 and the heat transfer speed of the semiconductor refrigeration plate 65, thereby improving the cooling effect of the cooling side of the semiconductor refrigeration plate 65, and also improving the efficiency of cooling the air in the metal coil 63, so that the temperature of the air entering the air inlet area 13 is lower than the warning temperature T0, thereby avoiding the interference of excessively high temperature air on the activity of the polypeptide and ensuring the quality of the concentrated polypeptide. This mechanism enables the polypeptide concentration device to have the function of concentration temperature control, improves the protection of the heat-sensitive polypeptide concentration process, thereby improving the quality of the heat-sensitive polypeptide after concentration, and at the same time improves the convenience and reliability of the use of the polypeptide concentration device;

[0048] When the polypeptide concentrating device is working, the ceramic filter cartridge 51 rotates slowly, the polypeptide liquid concentration process does not move violently, and the amount of foam generated on the surface of the polypeptide liquid is small. At the same time, after the air in the air inlet area 13 passes through the concentration channel and enters the water-permeable concentration membrane sleeve 52, the airflow can blow away a small amount of foam on the surface of the polypeptide liquid. In addition, the negative pressure suction force of the negative pressure area 14 will further suck the air in the foam on the surface of the polypeptide liquid and achieve the purpose of defoaming. The defoaming process does not require the addition of a defoaming agent. In addition, the ceramic filter cartridge 51 can not only drive the water-permeable concentration membrane sleeve 52 to rotate and clean itself, but also support and protect the water-permeable concentration membrane sleeve 52 to avoid contamination and damage on the back of the water-permeable concentration membrane sleeve 52. This mechanism enables the polypeptide concentrating device to have the function of automatic defoaming of the polypeptide liquid, thereby improving the reliability of the use of the polypeptide concentrating device.

[0049] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A polypeptide concentration device, comprising a hollow concentration tank (1), characterized in that: The bottom end of the hollow concentrating box (1) is fixedly connected to a support frame (2), and the inner wall of the hollow concentrating box (1) is fixedly connected to two partitions (3) at an angle, and the outer walls of the two partitions (3) are each provided with a mounting through hole, and the hole wall of the mounting through hole is fixedly connected to a first sealing bearing (4), and the inner walls of the inner rings of the two first sealing bearings (4) are fixedly connected to a concentrating mechanism (5); A cleaning mechanism (6) is fixedly connected to the upper surface of the hollow concentration box (1); The outer wall of the bottom end of the support frame (2) is fixedly connected with a PLC controller (7) and a concentration acceleration mechanism (8); A through hole is formed on the upper surface of the hollow concentrating box (1), and a guide hopper (9) is fixedly connected to the hole wall of the through hole, and the bottom end of the guide hopper (9) is located inside the feed end of the concentrating mechanism (5); A touch screen control panel (10) is fixedly connected to the outer wall of the hollow concentration tank (1).

2. A polypeptide concentration device according to claim 1, characterized in that: The concentrating mechanism (5) includes a ceramic filter cartridge (51) fixedly connected to the inner wall of the inner ring of the two first sealed bearings (4), the inner wall of the ceramic filter cartridge (51) is fixedly connected to a water-permeable concentrating membrane sleeve (52), the bottom end of the ceramic filter cartridge (51) is fixedly sleeved with an outer gear ring (53), the side wall of the hollow concentrating box (1) is provided with a fixing hole, and the hole wall of the fixing hole is fixedly connected to a discharge hopper (54), the outer wall of one of the partitions (3) is fixedly connected to a driving motor (55), the outer wall of the partition (3) is provided with a through hole, and the hole wall of the through hole is fixedly connected to a second sealing The driving end of the driving motor (55) passes through the second sealed bearing (56), the outer wall of the driving motor (55) is in sealed contact with the inner wall of the inner ring of the second sealed bearing (56), the driving end of the driving motor (55) is fixedly connected with a gear (57) meshing with the outer gear ring (53), the inner wall of the hollow concentration box (1) is fixedly connected with two transverse plates (58), the opposite sides of the two transverse plates (58) are fixedly connected with a sealing strip (59), and the arc surface of the sealing strip (59) is in sealed contact with the arc outer wall of the ceramic filter cartridge (51).

3. A polypeptide concentration device according to claim 2, characterized in that: The two partitions (3) divide the internal cavity of the hollow concentrating tank (1) into a feed area (11), a processing area and a discharge area (12), and the two transverse plates (58) divide the processing area into an air intake area (13) and a negative pressure area (14).

4. A polypeptide concentration device according to claim 3, characterized in that: The cleaning mechanism (6) includes a heat-insulating box (61) fixedly connected to the upper surface of the hollow concentration box (1), the inner wall of the heat-insulating box (61) is fixedly connected to a heat-insulating plate (62), the outer wall of the heat-insulating plate (62) and the outer wall of the heat-insulating box (61) are both provided with circular holes, and the hole walls of the two circular holes are fixedly connected to a metal coil (63), the upper surface of the heat-insulating box (61) is provided with a through hole, and the hole wall of the through hole is fixedly connected to a metal heat-conducting plate (64), the lower surface of the metal heat-conducting plate (64) is in contact with the upper surface of the metal coil (63), and the metal heat-conducting plate (64) is in contact with the upper surface of the metal coil (63). ) is fixedly connected to the upper surface of a plurality of semiconductor refrigeration sheets (65), the heat dissipation sides of the plurality of semiconductor refrigeration sheets (65) are fixedly connected to a heat dissipation mesh block (66), and the upper surfaces of the plurality of heat dissipation mesh blocks (66) are fixedly connected to a heat dissipation fan (67). The side of the heat insulation box (61) away from the metal coil (63) is fixedly connected to an air outlet pipe (68), the bottom end of the air outlet pipe (68) passes through the top end of the hollow concentration box (1) and is connected to the cavity of the air inlet area (13), and the pipe wall of the air outlet pipe (68) is fixedly connected to a temperature sensor (69).

5. The polypeptide concentration device according to claim 4, characterized in that: The concentration acceleration mechanism (8) includes a collection box (81) fixedly connected to the outer wall of the bottom end of the support frame (2); the upper surface of the collection box (81) is fixedly connected to a liquid inlet pipe (82) and a curved pipe (83); the top end of the liquid inlet pipe (82) is fixedly connected to the bottom end of the hollow concentration box (1); the top end of the liquid inlet pipe (82) is connected to the cavity of the negative pressure zone (14); the upper surface of the collection box (81) is fixedly connected to an air pump (84); the air inlet end of the air pump (84) is fixedly connected to the top end of the curved pipe (83); the air outlet end of the air pump (84) is fixedly connected to a return pipe (85); the air outlet end of the return pipe (85) is fixedly connected to the air inlet end of the metal coil (63); the upper surface of the collection box (81) is provided with a mounting hole, and the hole wall of the mounting hole is fixedly connected to a liquid level sensor (86).

6. The polypeptide concentration device according to claim 5, characterized in that: The bottom end of the side wall of the collection box (81) is fixedly connected to an electromagnetic drain valve (15), and the water outlet end of the electromagnetic drain valve (15) is fixedly connected to a drainage pipe (16).

7. The polypeptide concentration device according to claim 6, characterized in that: The bottom end of the side wall of the heat insulation box (61) is fixedly connected to a drainage branch pipe (17), and the bottom end of the drainage branch pipe (17) is fixedly connected to the pipe wall of the drainage pipe (16).

8. A method for concentrating the polypeptide using the concentrating device according to claim 7, characterized in that: The method comprises the following steps: Step S1: First, a start signal is sent to the PLC controller (7) through the touch screen control panel (10). The PLC controller (7) controls the driving motor (55) and the air pump (84) to start according to the received start signal. The driving motor (55) causes the ceramic filter cartridge (51) to rotate slowly. The air pump (84) causes the negative pressure zone (14) to be in a negative pressure environment. The air sucked by the air pump (84) enters the air inlet zone (13) through the return pipe (85) and the cleaning mechanism (6). Step S2: The polypeptide liquid is then poured into the concentration mechanism (5) through the guide hopper (9). Under the negative pressure suction of the negative pressure zone (14), the polypeptide liquid solution quickly passes through the micro-pores of the water-permeable concentration membrane sleeve (52) and the ceramic filter cartridge (51) and enters the negative pressure zone (14) of the hollow concentration box (1), thereby achieving the purpose of rapid concentration. Step S3: In step S2, the driving motor (55) drives the ceramic filter cartridge (51) to rotate slowly, and the cleaning mechanism (6) cooperates, so that the polypeptide liquid will never block the concentration channel during the process of being discharged to the bottom outlet of the ceramic filter cartridge (51); Step S4, before step S2, the staff presets the warning temperature T0 according to the temperature adaptability of the polypeptide itself, and the PLC controller (7) also controls the cooling fan (67) and the plurality of semiconductor refrigeration sheets (65) to be powered on and work. The plurality of semiconductor refrigeration sheets (65) cool the air entering the metal coil (63) through the metal heat conducting plate (64). While cooling the air, the air can also liquefy the moisture carried by the air. The cooled air enters the air outlet pipe (68) and is detected by the temperature sensor (69). Afterwards, the cleaning mechanism (6) regulates the power of the cooling fan (67) according to the temperature value detected by the temperature sensor (69), thereby adjusting the temperature of the air entering the air inlet area (13); Step S5, when the polypeptide concentration device is working, the ceramic filter cartridge (51) rotates slowly, the polypeptide liquid concentration process does not move violently, and the amount of foam generated on the surface of the polypeptide liquid is small. At the same time, after the air inlet area (13) passes through the concentration channel and enters the inside of the water-permeable concentration membrane sleeve (52), the air flow can blow away a small amount of foam on the surface of the polypeptide liquid. In addition, the negative pressure suction force of the negative pressure area (14) will further suck the air in the foam on the surface of the polypeptide liquid, thereby achieving the purpose of defoaming.

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